Abstract
A cpk mutant of Neurospora crassa with morphological alteration was obtained spontaneously during the cross between the wild-type and a glycerol utilizing cr-l strain. The growth rate of cpk was intermediate between the wild-type and cr-1 mutant strains. The cpk conidia contained a reduced level of carotenoid pigments as compared to the wild-type conidia. The cpk mutant had no detectable amount of cyclic adenosine 3′,5′-monophosphate (cAMP)-binding protein at all stages of growth tested. On a DEAE-Sephacel column chromatogram, protein kinase activity of the wild type was eluted at two peaks; the first peak was cAMP-dependent, and the second one was not. In contrast, the cpk strain had two peaks of cAMP-independent enzymes. It is suggested that cAMP-dependent protein kinase may be altered in the cpk mutant into a cAMP-independent type by an alteration of the regulatory subunit of this enzyme.
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Abbreviations
- cAMP:
-
Cyclic adenosine 3′,5′-monophosphate
- 8-N3-[3H] cAMP:
-
8-azido-[3H]cyclic adenosine 3′,5′-monophosphate
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Murayama, T., Uno, I., Hamamoto, K. et al. A cyclic adenosine 3′,5′-monophosphate-dependent protein kinase mutant of Neurospora crassa . Arch. Microbiol. 142, 109–112 (1985). https://doi.org/10.1007/BF00447052
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DOI: https://doi.org/10.1007/BF00447052