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Double-stranded regions in denatured DNA from mouse cells (1973)

Church, Robert B. ; Georgiev, Georgii P.

Springer

Molecular biology reports 1 (1973), S. 21-25

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ISSN: 1573-4978

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Abstract Approximately 2% of the DNA of the mouse genome reassociates at infinitely low C 0 t values, 10-7 to 10-6 moles 1-1 s. The melting profile of the reassociation product, which is resistant to nuclease S1 digestion, has been characterized by hydroxyapatite column chromatography. The properties of these nuclease resistant sequences suggest that they exist as ‘DNA-hairpins’ and that they originate from reverted base sequences within the genome.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF00357401

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