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Double-stranded regions in denatured DNA from mouse cells

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Abstract

Approximately 2% of the DNA of the mouse genome reassociates at infinitely low C 0 t values, 10-7 to 10-6 moles 1-1 s. The melting profile of the reassociation product, which is resistant to nuclease S1 digestion, has been characterized by hydroxyapatite column chromatography. The properties of these nuclease resistant sequences suggest that they exist as ‘DNA-hairpins’ and that they originate from reverted base sequences within the genome.

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Author notes

  1. Robert B. Church

    Present address: Division of Medical Biochemistry and Biology, University of Calgary, Calgary, Alberta, Canada

Authors and Affiliations

  1. Institute of Molecular Biology, Academy of Sciences of the U.S.S.R., Moscow, U.S.S.R.

    Robert B. Church & Georgii P. Georgiev

Authors
  1. Robert B. Church
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  2. Georgii P. Georgiev
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Church, R.B., Georgiev, G.P. Double-stranded regions in denatured DNA from mouse cells. Molecular Biology Reports 1, 21–25 (1973). https://doi.org/10.1007/BF00357401

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  • DOI: https://doi.org/10.1007/BF00357401

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