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A simplified method for in situ embedding of monolayer cultures for electron microscopy (1983)

Shea, Thomas B. ; Berry, Eugene S.

Springer

Methods in cell science 8 (1983), S. 41-44

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ISSN: 1573-0603

Keywords: cell culture ; in situ embedding ; electron microscopy

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary A simple method for the in situ embedding of cell cultures for electron microscopy has been developed. The procedure is carried out in its entirety within the culture flask, using only standard fixing and embedding reagents. Precoating of the supporting surface of the flask with alternative substrates is not required. This method permits precise orientation of samples with respect to the phase-contrast image of living cultures.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01834634

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