Summary
A simple method for the in situ embedding of cell cultures for electron microscopy has been developed. The procedure is carried out in its entirety within the culture flask, using only standard fixing and embedding reagents. Precoating of the supporting surface of the flask with alternative substrates is not required. This method permits precise orientation of samples with respect to the phase-contrast image of living cultures.
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Shea, T.B., Berry, E.S. A simplified method for in situ embedding of monolayer cultures for electron microscopy. Journal of Tissue Culture Methods 8, 41–44 (1983). https://doi.org/10.1007/BF01834634
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DOI: https://doi.org/10.1007/BF01834634