ISSN:
1570-7458
Keywords:
Cardiochiles nigriceps
;
Microgasterinae
;
Heliothis virescens
;
artificial diets
;
in vitro rearing
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The composition of an artificial medium and technical procedures used for in vitro rearing of the endophagous larval parasitoid Cardiochiles nigriceps Viereck (Hymenoptera, Braconidae), from post-germ band egg to the 2nd instar larva, are described. Amino acids, carbohydrates, salts, and vitamins were supplied in defined amounts as an aqueous solution which, when supplemented with 20 mg/ml of bovine albumin, 5 mg/ml of lactalbumin (enzymatic hydrolysate), 20% (v/v) fetal bovine serum, 20% (v/v) milk and 10% (v/v) chicken egg yolk, allowed for parasitoid growth and molting to the 2nd instar. Molting to the final instar was never observed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00341084
