Publication Date:
2021-07-15
Description:
In order to introduce genetic markers of four species of fishes, 80 samples of each species, i.e. Parastromateus niger, Scomberomorus comersoniannus, Trachionotus mookalee and Caranx para were collected. DNA was extracted using phenol- chloroform method. The target gene (cytochrome b) was amplified by Thermal cycle (PCR) and the PCR product size estimated 1105 bp. In this research out of 27 DNAase enzymes which were used for PCR product enzyme digesting 8 enzymes (Bam HI, Alw 261, Rsa I, Mbo I, Alu I, Hinf I, Dpn I, Dde I) have cut side on target DNA and three enzymes of them Alu I, Hinf I and Mbo I showed polymorphism genetic differences while other enzymes displayed similar patterns. Variation of haplotypes from four species are as follows: BAA for P. niger, AAB for T. mookalee, ABA for C. para, and ACA for S. comersonianus. So it is possible to claim that each of the above Haplotypes may be used as genetic markers for each of the species.
Keywords:
Biology
;
Genetics
;
DNA
;
Enzymes
;
Biomarkers
;
Identification
;
Phenotypes
;
Chloroform
;
Haplotypes
;
Gene polymorphism
;
Genetic markers
;
Caranx
;
Marine
;
Separation
;
Polymerase chain reaction
;
Phenotypic variations
;
Cytochrome b
;
DDE
;
Trachionotus mookalee
;
Parastromateus niger
;
Caranx para
;
Carangidae
;
Scomberomorus comersoniannus
;
Scomberomorus
;
ISW
;
Arabian Sea
;
Oman Gulf ISW
;
Persian Gulf
;
Iran
Repository Name:
AquaDocs
Type:
article
,
TRUE
Format:
application/pdf
Format:
application/pdf
Format:
1-14
