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  • paleolimnology  (54)
  • Immunocytochemistry  (35)
  • Springer  (89)
  • 1980-1984  (89)
  • 1925-1929
  • 1983  (89)
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  • Springer  (89)
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  • 1980-1984  (89)
  • 1925-1929
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  • 1
    ISSN: 1432-041X
    Keywords: Vitellin ; Yolk granule ; Yolk protein ; Silkworm ; Embryogenesis ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Vitellin was purified from eggs of the silkworm,Bombyx mori, by a new method in which vitellin was extracted from isolated yolk granules. The purified vitellin had a molecular weight of 540,000. An antibody against purified vitellin was prepared in rabbits. It reacted with the hemolymph vitellogenin as well as with purified vitellin, but not with other proteins in the hemolymph or in the extract from yolk granules. The anti-vitellin IgG was used to immunocytochemically locate vitellin in theBombyx non-diapause egg during early developmental stages. In the egg, just after oviposition, vitellin was located in internal yolk granules and in small yolk granules of the periplasm. During the early developmental stages studied, vitellin was not metabolized uniformly throughout the egg. The vitellin of the internal yolk granules located at the posterior-dorsal part and of the small peripheral yolk granules was utilized in 16 h and 2 days, respectively, after oviposition. A thin, very vitellin-poor layer was located between the periplasm and the vitellin-rich interior in the newly laid egg. it was always in close contact with the periphery where blastoderm and germ-band cells developed.
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  • 2
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    Hydrobiologia 98 (1983), S. 223-235 
    ISSN: 1573-5117
    Keywords: diatoms ; paleolimnology ; lakes ; Minnesota ; sediments ; microfossils
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Fossil diatom assemblages from the sediment/water interface in 105 Minnesota lakes were compared with measurements of alkalinity, sulphate, total phosphorus, transparency, and water depth at the sample site. Similar assemblages were placed together using cluster analysis and comparisons of environmental variables between diatom clusters were made using an analysis of variance. Total alkalinity and transparency showed the greatest difference among clusters. Samples from shallow eutrophic prairie lakes were dominated by Melosira granulata, Stephanodiscus niagarae and, occasionally, by Stephanodiscus hantzschii. Deep oligotrophic lakes had modest percentages of Cyclotella comta. Dilute acid lakes were dominated either by Melosira distans and M. italica or by Tabellaria fenestrata, Cyclotella stelligera, and in some cases C. glomerata. Assemblages with Cyclotella glomerata and Synedra nana were found in naturally meromictic lakes. Stephanodiscus hantzschii showed a preference for extremely eutrophic lakes. The relationships between recently deposited diatom assemblages and the lake environmental conditions studied here can be used to evaluate the extent of past environmental change in lakes.
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  • 3
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    Hydrobiologia 103 (1983), S. 65-69 
    ISSN: 1573-5117
    Keywords: paleolimnology ; X-ray radiography of sediment cores ; sedimentary properties ; sediment accumulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract X-ray radiographs of unextruded sediment cores are used for the documentation and the interpretation of primary and secondary sedimentary structures, and for the correlation of synchronous laminations between different cores. The vertical variation in bulk density, water content and void ratio, and the increase of solids with increasing sediment depth are calculated from the recorded film density along the radiographs. The X-ray radiographic technique for the study of sedimentary properties is fast and non-destructive. The technique is especially valuable when studying the uppermost part of the sediment cover and the processes of sediment redistribution and sediment accumulation. Studies are being undertaken in several Swedish lakes and coastal bays, where mono- and stereoradiographs are being used to classify bottom and sediment types, to interpret changes in the depositional environment, and to calculate past and present rates of sediment accumulation.
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  • 4
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    Hydrobiologia 103 (1983), S. 75-79 
    ISSN: 1573-5117
    Keywords: paleolimnology ; Dead Sea climate ; pore-waters ; diffusion ; sediments ; salinity ; porosity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The salinity of lakes is subject to variations imposed by climatic changes. These variations are recorded in the salinity profile of pore waters. Meromictic lakes, such as the Dead Sea, are a special case where waters which underlie the mixolimnion reflect salinity variations. In a sediment core from Dead Sea shallow waters, the salinity profile exhibited a minimum at about 1.9 m depth. It is shown by a diffusion model that this minimum can be attributed to lower salinities which prevailed at the sediment water interface for several decades around the turn of this century. No such minimum was observed in a sediment core from the deepest part of the lake where, during the last two centuries, the overlying brines had a constant salinity.
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  • 5
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    Hydrobiologia 103 (1983), S. 81-84 
    ISSN: 1573-5117
    Keywords: paleolimnology ; geochemistry ; trace elements
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A geochemical study of lacustrine sediments was undertaken as part of a major paleolimnological project concerned with the impact of man on lakes. Factor analysis was applied to the geochemical data obtained from a core from one lake. Three principal factors which explained most of the variance of the initial data were identified. Factor I is related to the organic:mineral component ratio of the sediment, and can be considered as an indirect index of change in lake trophic status through time. Factor II is related to the granulometric composition of the sediment and matches evidence for changes in the hydrological regime of the lake. Factor III reflects changes in redox potential and is more closely related to processes occurring within the lake than other factors. Specific geochemical associations of elements are connected with each factor and are the factor indicators. The correlation of these associations are analyzed as geochemical indices of the variability of sediment accumulation conditions in time.
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  • 6
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    Hydrobiologia 103 (1983), S. 85-90 
    ISSN: 1573-5117
    Keywords: paleolimnology ; diatoms ; East Africa ; transfer functions ; pH-indicators
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Our purpose is to establish the quantitative relationship between recent diatom floras and ecological parameters, in order to extrapolate the results to the past. The parameter pH is here considered as an example. This work is based on the study of about I60 diatom samples from East Africa and of their corresponding biotopes. We propose some statistical methods to interpret the data. Correspondence analysis allows us to define the pH-indicator species. The regression calculations allow pH values to be calculated using the percentage of the diatom species in a sample.
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  • 7
    ISSN: 1573-5117
    Keywords: paleolimnology ; caesium-137 ; perched lake ; meromictic ; diffusion ; bioconcentration ; sediment accumulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Hidden Lake is a perched, brown-water lake located in the centre of Great Sandy Island (Fraser Is.), S.E. Queensland. It is highly acid (pH 4.0), oligotrophic and is thermally and chemically stratified for most of the year. The sediments revealed a 137Cs profile which departed from the temporal pattern of 137Cs fallout in Brisbane and was represented by an exponential increase of 137Cs towards the surface sediments from ca. 32 cm depth. The possible causes of the divergent profile are discussed, including physical and biological mixing, lag in the transport of catchment material to the sedimentary basin, diffusion, recycling and biological concentration. It is hypothesised that a combination of the last four processes, with diffusion facilitated by the highly acid conditions, are the major causes of the observed 137Cs profile. Possible recycling and bioconcentration of 137Cs raises questions as to the validity of this method of dating in similar environmental conditions, and as to the interpretation of other palaeochemical data. These hypotheses are to be tested against profiles obtained from 14C, 210Pb, 239/240Pu analyses of the sediment, and the measurement of 137Cs activity in the water and biota of the lake.
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  • 8
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    Hydrobiologia 103 (1983), S. 45-51 
    ISSN: 1573-5117
    Keywords: paleolimnology ; magnetism ; dating ; Australia ; North America ; Japan ; Europe ; Near East
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Paleolimnomagnetic records from five regions of the world have been combined with historical magnetic field observations in order to produce regional geomagnetic master curves.
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  • 9
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    Hydrobiologia 103 (1983), S. 37-44 
    ISSN: 1573-5117
    Keywords: paleolimnology ; magnetic minerals ; sediment sources
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This review outlines the origin and environmental significance of magnetic minerals in lake sediments. Attention is drawn to situations where the patterns of mineral magnetic variation is a reflection of processes other than changing erosion rates and fire incidence. The use of mineral magnetic techniques in sediment source tracing, palaeoclimatic studies and the reconstruction of particulate pollution history is illustrated by means of case studies from Britain and N. America. The value of magnetic susceptibility as an on-site core logging technique is shown by reference to data from Lake Washington.
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  • 10
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    Hydrobiologia 103 (1983), S. 53-58 
    ISSN: 1573-5117
    Keywords: paleolimnology ; secondary ferrimagnetic oxides ; S.I.R.M. ; magnetic susceptibility ; laminated sediments
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The magnetic parameters S.I.R.M. and magnetic susceptibility have been used to try and establish the fire histories of lake drainage basins. The technique is demonstrated using sediments from three lakes: Llyn Bychan (N. Wales), a lake with a recently burnt catchment, Lake Biscarrosse (S. W. France), a lake with a well documented fire history, and Lake Laukunlampi (E. Finland), a lake with laminated sediments and a long, but unknown fire history.
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  • 11
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    Hydrobiologia 103 (1983), S. 141-146 
    ISSN: 1573-5117
    Keywords: paleolimnology ; lakes ; sediments ; eutrophication ; Cladocera ; Switzerland
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Species composition and density of cladoceran populations changed in Lakes Zürich, St. Mortiz and Baldegg as human populations increased in these watersheds. Lake Zürich sediments became annually laminated in the 1890's as a result of increased organic input as the size of the cities surrounding the lake grew. At the same time, the Bosmina species changed from a oligotrophic form (longispina) to a eutrophic form (longirostris). An increase in Daphnia spp. populations also occurred at this time in the lake's history. Bosmina longispina reappeared in the lake in 1965 as the lake's trophic status changed from eutrophic to mesotrophic due to effective sewage treatment facilities. Annual laminations appear in the Lake St. Moritz sediments about 1910. Shortly thereafter, a shift from B. longispina to B. longirostris occurred. This change in trophic status is associated with increased tourism in the area. Lake Baldegg sediments also show annual laminations beginning in 1885 and a similar shift in the Bosmina species. Other cladoceran remains were too scarce to be useful in interpreting the histories of these lakes.
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  • 12
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    Hydrobiologia 103 (1983), S. 59-64 
    ISSN: 1573-5117
    Keywords: paleolimnology ; sedimentation pattern ; magnetic susceptibility ; Scanian lakes ; palaeomagnetism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Magnetic susceptibility measurements have been used to correlate synchronous depths in ca. 50 l m. cores of recent sediment from a small lake (area ca. 55 ha) in Scania, southern Sweden. The three-dimensional picture of sediment accumulation which emerges provides a basis for studying sediment deposition patterns through time. Using a palaeomagnetic chronology, the results show that the pattern and rate of accumulation have dramatically altered during the past 350 years, thus making semiquantitative studies of downcore sediment properties in a single core problematical. Possible reasons for these phenomena are briefly discussed.
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  • 13
    ISSN: 1573-5117
    Keywords: paleolimnology ; sediment core positioning ; hydrographic survey ; computer contouring ; morphometric calculation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Automated techniques for sediment core site positioning, hydrographic survey, and contour mapping are described. The computer contouring system can be extended to calculate values for morphometric variables such as maximum length (1max), maximum width (wmax), lake perimeter (10), lake area (A) and lake volume (V). The techniques are exemplified using results from Augher Lough, Northern Ireland.
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  • 14
    ISSN: 1573-5117
    Keywords: paleolimnology ; diatoms ; salinity ecology ; annual lamination ; sulphide mud ; meromixis ; Baltic
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Diatoms have been studied in an annually laminated sulphide mud which was deposited after the impoundment of the sea-bay of Gennarbyviken. It was possible to follow how the measured decrease in salinity in the basin was reflected by the diatom flora of the sediment which had been deposited concurrently
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  • 15
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    Hydrobiologia 103 (1983), S. 153-158 
    ISSN: 1573-5117
    Keywords: paleolimnology ; eutrophication ; reservoirs ; Bosmina ; Chironomidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Eutrophication of reservoirs can be detected by changes in the abundance of insect and crustacean remains in the sediments. In recently constructed reservoirs, the time of impoundment can be determined through the presence of chironomid head capsules. The initial phase is characterised by highly enriched water as can be seen by the abundance of Bosmina remains. If further nutrient input into the reservoir remains low, the upper part of the sediment column is almost completely lacking in invertebrate remains.
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  • 16
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    Hydrobiologia 103 (1983), S. 159-163 
    ISSN: 1573-5117
    Keywords: paleolimnology ; marine/brackish-freshwater ; transition basin ; wildfowl ; gregarious waterfowl ; allochthonous nutrients ; seasonal eutrophication
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A new and very special course of events starts in a freshwater basin, just isolated from the sea. In NW Europe this is mainly caused by land uplift in Holocene times or by regression of the sea. It has been thoroughly documented by the gradual change in the composition of the fossil diatom floras. Attention is drawn to the possible impact of migrating birds, especially wildfowl, breeding, resting and moulting in those often shallow, sheltered lakes along the Scandinavian coastlines. The gregarious wildfowl species might seasonally increase the allochthonous input of nutrients in the lake ecosystems and cause marked changes in biotic interactions. A working hypothesis is suggested, that the wildfowl species in question were appreciably more numerous before the arrival of man. Consequently, their effect on seasonal eutrophication cannot be neglected in the paleolimnological context.
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  • 17
    ISSN: 1573-5117
    Keywords: paleolimnology ; lakes ; eutrophication
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Problems relating to the disappearance and eutrophication of lakes as a result of human activity are presented. A study area was selected located in an agricultural/forest region where human influence on lakes used to be minimal. In the past 25 years a number of lakes have completely disappeared and others have become rapidly enriched. Human interference has included: the pulling down of water mills, drainage and river canalisation, construction of holiday resorts, development of intensive farming, increased mineral fertilization, and the extension of roads and road traffic.
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  • 18
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    Hydrobiologia 103 (1983), S. 177-179 
    ISSN: 1573-5117
    Keywords: paleolimnology ; sediment chemistry ; heavy metals ; ATP
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This investigation is concerned with the impact of industrial and municipal waste discharge on lakes near the city of Tampere, Finland. The record of P, Zn and ATP in the recent sediments of Vanajavesi I, a polluted lake, and Mallasvesi, a largely unpolluted lake, are compared.
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  • 19
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    Hydrobiologia 103 (1983), S. 247-250 
    ISSN: 1573-5117
    Keywords: paleolimnology ; Pannonian lakes ; infilling process ; environmental change ; hydrosere
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Data on the stratigraphy and development of a former lake near the town of Vracov, SE Moravia, Czechoslovakia, are presented. The infilling process and vegetation succession are described. An attempt to reconstruct changes in habitat conditions during the last ca. 13 000 years has been made.
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  • 20
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    Hydrobiologia 103 (1983), S. 241-245 
    ISSN: 1573-5117
    Keywords: paleolimnology ; diatom succession ; lake development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Late-glacial and Postglacial sediments of the former Komořany Lake in North-West Bohemia were studied by means of diatom analysis to trace the pattern of past environmental change in the lake. Several phases of lake development could be distinguished. Originally the Komořany Lake was a eutrophic shallow water basin. The diatom succession in the early Postglacial sediment indicates alternating alkaliphilic, epiphytic and planktonic diatom communities associated with changes in water level in the basin. At the beginning of the Subatlantic period the water level began to rise and the development of planktonic diatoms, typical of small eutrophic lakes, occurred. Later, the basin became shallow again and the number of indifferent and acidophilous diatoms increased. This is typical for the final phase of the infilling process of a lake before its transformation to peatland
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  • 21
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    Hydrobiologia 103 (1983), S. 257-260 
    ISSN: 1573-5117
    Keywords: paleolimnology ; palynology ; bacteriology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract There are many lake basins in the Soviet Union, both in the humid and the arid regions, including lowland and alpine, and shallow and deep-water lakes. Lithological, geochemical, radiological and paleobiological methods have been utilized in studying the history of these lakes. Lake Zaisan in Kazakhstan is described as an example of lake development in the arid zone, whilst the lake systems of the Pribaltica, where human influence on the environment is clearly recorded, exemplifies the humid zone.
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  • 22
    ISSN: 1573-5117
    Keywords: paleolimnology ; diatom flora ; sediments ; Vistula Lagoon ; Poland
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The variability of Actinocyclus kuetzingii (A.S.) Sim. in the Littorina deposits of the Vistula Lagoon and Lake Druzno were studied. During the time of the Littorina Lake Druzno was a part of the Vistula Lagoon. In the different parts of the Lagoon both the abundance of A. kuetzingii and the diameter of its valves change in a manner associated with phases of the Littorina transgression. Coscinodiscus (Actinocyclus) kuetzingii fo. subsalsa Brockmann is probably an ecotype of A. kuetzingii (A.S.) Sim.
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  • 23
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    Hydrobiologia 103 (1983), S. 281-285 
    ISSN: 1573-5117
    Keywords: paleolimnology ; Lake Baikal ; Pliocene ; Pleistocene ; pollen ; diatoms
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The following stages in the formation of sediments in Lake Baikal have been distinguished. In the Middle Miocene lacustrine deposits accumulated in the southern depression. In Early and Middle Pliocene, as well as in Early-Middle Pleistocene the bottom of the southern and middle Baikal depressions was occupied by large lakes, but the northern basin was mainly dry with a system of small lakes, streams and rivers. This accounts for the great facies variety and the genetic heterogeneity of sediments in the northern depression. At the end of Middle and in the Late Pleistocene an abrupt warping of the depression took place, and a shore-line similar to the present one was formed. Sands in the upper part of the sediments are considered to be the product of intensive erosion which probably occurred in the pluvial epoch in the Late Pleistocene period.
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  • 24
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    Hydrobiologia 103 (1983), S. 287-293 
    ISSN: 1573-5117
    Keywords: paleolimnology ; sedimentation ; sediment laminations ; diatom microstratigraphy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Laminated sediment is deposited in the deep areas (〉 30 m) of Lake Pääjärvi. In the diatom microstratigraphy a regular alteration of planktonic and littoral floras, which goes in pace with the visual structure, reflects the yearly cycle of sedimentation. Considerable variation in sedimentation was found to have occurred during the past two decades. A sequence, dated to the early 1960's, in which the organic content was exceptionally high, could be identified in the sediment at 6–14 cm depth even in shallower areas where laminations are not formed. This level was used as a marker horizon when determining the recent distribution of sediment by means of 19 cores taken from different parts of the lake. The average annual sediment accumulation in the profundal zone of the lake is 340 g m−2a−1 dry weight, of which 25 g m−2 a−1 (1100 kJ m−2 a−1) is organic carbon.
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  • 25
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    Hydrobiologia 103 (1983), S. 303-308 
    ISSN: 1573-5117
    Keywords: paleolimnology ; acid waste-water ; liming ; diatoms ; heavy metals
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Lake Sysmäjärvi is located inside the municipal boundary of Outokumpu in North Karelia, Finland. Since 1928 metalliferous, and at a later stage highly acid waste-water, has been discharged into the lake. Neutralization of the lake water by liming was introduced, and by the end of 1966 the pH of the water had become neutral to alkaline. Two sediment cores (0.5 m and 5 m long, respectively) were analyzed for pollen, diatoms, loss-on-ignition, organic carbon, Fe, Cu, Ni, Pb, Zn and Hg. The maximum concentrations of organic matter and heavy metal content occur 30 cm below the sediment surface. Evidence of the liming and of accelerated eutrophication appear close to the surface of the sediment (0–10 cm), indicated by an increase in Nitzschia plana.
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  • 26
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    Hydrobiologia 103 (1983), S. 295-301 
    ISSN: 1573-5117
    Keywords: paleolimnology ; Flandrian biostratigraphy ; Tabellaria binalis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Early and Middle Flandrian geological development and paleolimnology of Lake Spitaalijärvi was studied using pollen, diatom and cladoceran analysis and 14C dating. Spitaalijärvi was isolated from the Ancylus Lake about 9000 B.P., at which time birch and pine and plants typical of open habitat communities grew on the solitary island. The rational limits for Alnus and Picea were ca. 8300 B.P. and 3700 B.P., respectively. During the first few hundred years after isolation Spitaalijärvi was probably fairly eutrophic, with a low water level. Water level began to rise before the Alnus rise (A°) and the lake became oligotrophic. After another transgression, which started before the Picea rise (P°), Tabellaria binalis and Semiorbis hemicyclus appear in the diatom stratigraphy indicating ultra-oligotrophic conditions. The main reason for the ultra-oligotrophy of Lake Spitaalijärvi is the character of the parent material in the catchment, consisting of glacial and littoral deposits derived from the local quartz sandstone of highly siliceous nature.
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  • 27
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    Hydrobiologia 103 (1983), S. 309-318 
    ISSN: 1573-5117
    Keywords: paleolimnology ; heavy metals ; polluted lake ; sedimentary chemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Macro nutrients N, P, K, Ca, Mg, Na and S as well as heavy metals Pb, Cu, Zn, Co, Cr, Ni, Fe and Mn were analysed from a sediment core representing a 7000-year long history of a lake including the preceding brackish (Litorina Sea) and freshwater (Ancylus Lake) stages. Sediments were also analysed for sedimentary pigments, algal growth potential (AGP) of the interstitial water, phosphorus fractions, pH, specific conductivity and redox potential. The annual accumulation values of several chemical elements were interpreted in light of other paleolimnological data from the same core. In the uppermost sediment stratum the influence of sewage pollution is clearly reflected by increases in total electrolyte content, pH, AGP and sedimentary pigments including myxoxanthophyll. Nickel, copper and manganese only slightly increase in the recent sediment, whilst other heavy metals (especially Cr and Zn) and sulphur, phosphorus, nitrogen and total organic matter are heavily concentrasted in the polluted surface sediment. The interpretation of the chemostratigraphy of the lake accords with evidence of lake development based on biological analyses.
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  • 28
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    Hydrobiologia 103 (1983), S. 15-19 
    ISSN: 1573-5117
    Keywords: paleolimnology ; dating ; radioactive fallout ; sediment ; 137Cs ; 239,240Pu ; varves
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The distribution of 137Cs and 239,240Pu in sediment core samples of the Finnish lakes Laukunlampi, Lovojärvi and Pääjärvi were determined. The sediment samples were collected using dry ice and liquid nitrogen freezing methods. The sediments of these lakes are annually laminated. A clear maximum concentration of 137Cs and 239,240Pu was found in sediment layers formed during 1962–1964, the years of maximum fallout, and the middle of the 1950's can be estimated from the 137Cs and 239,240Pu profiles. The highest concentrations, 11 500 and 820 pCi kg−1 dry wt for 137Cs and 239,240Pu, respectively, were found in the sediment of Laukunlampi. The vertical distribution was similar for 137Cs and 239,240Pu in the lakes investigated. A slight migration of 239,240Pu and 137Cs was found and the migration of 137Cs seems to be higher than that of 239,240Pu. The advantages of 137Cs dating method are rapidity and simplicity. 239,240Pu is preferable when the sample size is small. The agreement found between 137Cs and 239,240Pu dates and the annual laminae show that these fallout radio isotopes can be used for dating sediments formed during the past 25 years.
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  • 29
    ISSN: 1573-5117
    Keywords: Chrysophycea ; cultural eutrophication ; paleolimnology ; resting cyst ; statospore ; stratigraphy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The recent sediments of Frains Lake, Michigan contain a rich and well preserved association of chrysophycean cysts. Forty one forms are revealed by scanning electron microscopy (SEM) and light microscopy (LM). Taxonomic descriptions. and SEM micrographs are provided for the dominant forms. The three dominant taxa throughout the sediments, Cysta minima, C. modica and C. subbavaricum, do not show significant shifts in proportional abundance associated with European settlement and the onset of cultural eutrophication. However, certain subdominant taxa do show clear trends. Density counts indicate a dramatic decline in cyst concentration (by volume and by dry mass) and a sharp increase in absolute accumulation (net annual influx) following settlement. The Frains Lake profile of chrysophycean cysts is compared to sequences of other North American and European temperate lakes. The utility of chrysophycean cysts as paleoenvironmental indicators is considered on the basis of these results.
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    Hydrobiologia 103 (1983), S. 91-97 
    ISSN: 1573-5117
    Keywords: paleolimnology ; diatoms ; assemblage indicators ; Finland
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    Topics: Biology
    Notes: Abstract Diatom assemblages from the topmost sediment of 49 lakes were ordinated by means of a program for detrended correspondence analysis and reciprocal averaging (Decorana). Five lake groups were separated, each having more or less characteristic diatom assemblages and water quality. Shifts from one group to another caused by factors such as acidification, artificial drainage, diffuse nutrient loading and sewage are discussed.
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    Hydrobiologia 103 (1983), S. 99-102 
    ISSN: 1573-5117
    Keywords: paleolimnology ; organic matter ; bacteria ; aerobic decomposition ; oxygen consumption
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    Notes: Abstract The investigations were carried out in lakes situated in the forest zone (Karelian Isthmus, Baltic zone, South Ural) and in the forest-steppe (South Ural) of the USSR. The lakes differ in their bioproductivity and in the intensity of their human influence. The amount of organic matter accumulating in the sediments is closely related to production and decomposition processes in the trophogenic layer. Processes of organic matter transformation were found to be most active in the uppermost sediment. The quantity of bacteria shows no correlation with the organic matter content of the sediment. Increase in organic matter, up to 70–80% of dry weight, is often accompanied by a decrease in bottom bacteria. The intensity of aerobic decomposition of the labile organic matter can be judged from the oxygen demand of the sediments. However, it is important to differentiate between chemical and biological oxidation processes. The quantity of bottom bacteria correlates closely with the value of oxygen consumption only in cases of high sediment redox potentials.
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    ISSN: 1573-5117
    Keywords: paleolimnology ; sedimentary pigments ; primary production ; core analyses
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    Notes: Abstract A statistically significant correlation of the type y = axb between sedimentary plant pigments and contemporary algal primary production has been found in a study of the recent trophic evolution of twelve Italian lakes. The equation is used to assess baseline production levels in periods (50–70 years ago) when human influence was low.
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    Hydrobiologia 103 (1983), S. 107-111 
    ISSN: 1573-5117
    Keywords: paleolimnology ; lake ontogeny ; lake succession ; eutrophication
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    Topics: Biology
    Notes: Abstract Paleolimnologists frequently invoke the term ‘eutrophication’ for describing apparent enrichment phases in the history of a lake. I argue that this term is often used incorrectly and that alternative explanations can serve as more accurate descriptions. Increased organic content in the sedimentary record may result from increased nutrient availability (eutrophication), but it can also reflect decreased residence time of water, or changes in biotic interactions, or changes in lake morphometry. Additionally, I argue that ‘eutrophication’ is an inappropriate term for describing the aging process of lakes. Lake ontogeny is the preferred term, as it does not imply directional changes in nutrients, nor in community structure.
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    Hydrobiologia 103 (1983), S. 125-130 
    ISSN: 1573-5117
    Keywords: paleolimnology ; pollen ; diatoms ; Synuraceae ; Chrysophyta ; meromixis ; eutrophication
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The recent history of Little Round Lake, a small meromictic lake in southeastern Ontario, is considered. Pollen analyses were used to identify past changes in terrestrial vegetation, whilst limnological conditions were interpreted on the basis of diatom and chrysophyte microfossils. Contemporaneous with the arrival of European settlers (ca. A. D. 1850), the predisturbance assemblage of oligotrophic Cyclotella diatoms was replaced by Synedra spp., which then succeeded to a eutrophic flora dominated by Stephanodiscus hantzschii. Meanwhile, synuracean algae were almost completely excluded. Over the last 30 years, the algal microfossils indicate that the lake underwent a marked return to oligotrophy. Evidence is presented which suggests that this shift was related to the cultural enhancement of meromixis by the seepage of road salt.
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  • 35
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    Keywords: paleolimnology ; acidic precipitation ; lake acidification ; heavy metal pollution ; diatoms ; cladocerans
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    Notes: Abstract Sediment cores from nine lakes in southern Norway (N) and six in northern New England (NE) were dated by 137Cs, 210Pb and in NE also by pollen, and were analyzed geochemically and for diatoms. Cores from two N and three NE lakes were analyzed for cladocerans. 137Cs dating is unreliable in these lakes, probably due to mobility of Cs in the sediment. In Holmvatn sediment, an up-core increase in Fe, starting ca. 1900, correlates with geochemical indications of decreasing mechanical erosion of soils. Diatoms indicate a lake acidification starting in the 1920's. We propose that soil Fe was mobilized and runoff acidified by acidic precipitation and/or by soil acidification resulting from vegetational succession following reduced grazing. Even minor land use changes or disturbances in lake watersheds introduce ambiguity to the sedimentary evidence relating to atmospheric influences. Diatom counts from surface sediments in 36 N and 31 NE lakes were regressed against contemporary water pH to obtain coefficients for computing past pH from subsurface counts. Computed decreases of 0.3–0.8 pH units start between I890 and I930 in N lakes already acidic (pH 5.0–5.5) before the decrease. These and lesser decreases in other lakes start decades to over a century after the first sedimentary indications of atmospheric heavy metal pollution. It is proposed that the acidification of precipitation accompanied the metal pollution. The delays in lake acidification may be due to buffering by the lakes and watersheds. The magnitude of acidification and heavy metal loading of the lakes parallels air pollution gradients. Shift in cladoceran remains are contemporary with acidification, preceding elimination of fishes.
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    Hydrobiologia 103 (1983), S. 131-133 
    ISSN: 1573-5117
    Keywords: paleolimnology ; recent sediments ; diatoms ; chrysophytes ; 210lead
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    Notes: Abstract Diatom and chrysophyte remains in recent sediments have been examined. The upper stratigraphy is dated by reference to phytoplankton records whilst the lower depends on dates from 210Pb analysis, which shows some unexplained variation. Recent changes, due to farming and domestic drainage, form part of the long term response of the lake to events upon the catchment.
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    Hydrobiologia 103 (1983), S. 135-139 
    ISSN: 1573-5117
    Keywords: paleolimnology ; Traunsee ; artificial meromixis ; ostracods
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    Topics: Biology
    Notes: Abstract Traunsee is the deepest (189 m) lake in Austria and has been holomictic until the beginning of industrial discharges from the alkali works in Ebensee. The input of soluble compounds such as CaCl2 and NaCl have produced meromictic conditions. The relatively high oxygen content of the monimolimnion is due to water movements promoted by the large inflow and wind-action. The industrial discharges have caused both meromictic conditions and the deposition of highly alkaline sediment in the southern portion of the profundal zone. The effect of the discharges on the benthic fauna was evaluated by comparing the subfossil benthic fauna of four cores taken inside and outside the affected area. The ostracod fauna resembles that of other large lakes in the Salzkammergut, but also includes groundwater species such as Kovalevskiella and Cypridopsis subterranea. Leucocythere mirabilis has been found for the first time in an Austrian lake. Ostracods and other benthic organisms such as Testacea were not apparently influenced by the meromictic condition. They are, however, missing in all areas covered by the alkaline sludge.
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    Hydrobiologia 103 (1983), S. 169-175 
    ISSN: 1573-5117
    Keywords: paleolimnology ; Chironomidae ; subfossil remains ; shallow lake ; eutrophication ; Lake Balaton
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    Notes: Abstract Lake Balaton, the largest shallow lake in Central Europe, is about 20 000 years old. An enormous increase in tourism and the disproportionate building development of the last few decades has resulted in the acceleration of eutrophication in the lake. Widespread research to reveal the causes of water-quality deterioration and possible ways of protection against it have recently started. The investigation of the larvae of non-biting midges (Diptera: Chironomidae) in the sediment of the open-water zone has also begun. The contemporary faunal composition strongly correlates with the trophic gradient along the longitudinal axis of the lake. We therefore supposed that the eutrophication process should be identifiable from the analysis of subfossil chironomid head capsules from the upper (15 cm thick) layer of the sediment. We found that quantitative results could only be obtained when fragments as well as relatively intact head capsules are considered. Our data verify that the originally oligo-mesotrophic community has been gradually replaced by eutrophic species in a west to east direction. Large-bodied larvae belonging to the Chironomus plumosus group mix the sediment down to 15 cm as they build their tubes and consequently alter the original proportions of head capsules at the different levels. So the sequence of communities through the sediment-layers is not quite reliable.
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    Hydrobiologia 103 (1983), S. 181-184 
    ISSN: 1573-5117
    Keywords: paleolimnology ; Clostridium perfringens ; sewage pollution ; eutrophication
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    Notes: Abstract The horizontal and vertical distribution of the gram-positive, non-motile, spore-forming and rod-shaped bacterium Clostridium perfringens Holland was studied. The aim of the study was to estimate the quantity of C. perfringens at different depths of the sediment and evaluate the effect of human effluent which the lake received between 1940 and 1956. C. perfringens lives in the colon of man. Because it is spore forming and cannot multiply under a temperature of 20 °C and, according to the studies of Seppänen et al. (1979) it can be at least 300 years old, it may be a suitable paleolimnological indicator of pollution by human effluent. The results showed that the amounts of Clostridium increased at the same level where redox potential decreased in the sediment due to the beginning of effluent disposal at a depth of 40 mm. The maximum number of Clostridium colonies occurred between 0–30 mm depth.
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    Hydrobiologia 103 (1983), S. 193-198 
    ISSN: 1573-5117
    Keywords: paleolimnology ; Lake Michigan ; geochemistry ; stratigraphy ; Quaternary ; trace elements
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    Topics: Biology
    Notes: Abstract The stresses placed on Lake Michigan since the advent of industrialization require knowledge of the sedimentology of the whole lake in order to make informed decisions for environmental planning. Sediment accumulation rates are low: areas of the lake receiving the most sediment average only 1 mm a−1; deep-water basins average 0.1 to 0.5 mm a−1; and large areas are not receiving any sediment. Sediment was deposited rapidly (typically 5 mm a−1), in the form of rock flour, during the deglaciation of both Lake Michigan and Lake Superior Basins. Then the rate of accumulation decreased by 80–90% and has remained relatively constant since final deglaciation. Because active sedimentation occurs mostly in the deep water areas of the lake, the sediment remains undisturbed and contains a record of the chemical history of the lake.
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  • 41
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    Keywords: paleolimnology ; annual laminations ; magnetism ; sediment influx ; sediment chemistry ; eutrophication
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    Notes: Abstract The sediments of Loe Pool, a eutrophic coastal lake in south west England, consist largely of laminated clays and clay-gyttjas. Studies of the diatom microstratigraphy of frozen sediment cores from the Pool indicate that the laminations are annual, and that they contain pairs of light and dark bands formed by seasonal variations in the supply of sediment to the Pool from its catchment. Analysis of the magnetic properties of individual laminations demonstrates the presence of physical and mineralogical microstratigraphic variations, which may also be related to seasonality. A varve chronology, which is confirmed by 137Cs analysis and historical records, has been used to provide a timescale for the interpretation of data from other paleolimnological studies. A close agreement between variations in the abundance of sedimentary Sn, and the history of mining in the catchment, has been found. Similarly, analysis of total organic matter, total phosphorus, sedimentary chlorophyll a, sterols, diatoms and Cladocera in the uppermost sediments all indicate eutrophication of the Pool in the period AD 1940 to the present.
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    Hydrobiologia 103 (1983), S. 199-203 
    ISSN: 1573-5117
    Keywords: paleolimnology ; clay ; cultural disturbance ; erosion ; Guatemala ; Mayan archaeology ; silt ; particle-size analysis ; tropical lakes
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    Topics: Biology
    Notes: Abstract Long-term changes of sedimentary particle-size distribution in two tropical lowland lakes were compared with changes of human population sizes, estimated archaeologically, in the drainage basins. Mean particle size of silt and clay fractions (〈64 µm) varied between 3 and 15 µm. High positive skewness and kurtosis of the distributions were associated with smaller particle sizes; hence small mean size resulted from greater influx of small particles while influx of larger particles was probably constant. An inverse correlation between mean particle size and human population size is interpreted to mean that disturbance-induced erosion results in delivery of very fine inorganic particles at higher rates. Within any one basin, particle-size stratigraphy is more precisely related to archaeological time periods than is pollen stratigraphy. An absolute chronology still eludes us, owing to the failure of 14C dating of calcareous, colluvial sediments, but our relative chronology is now more precise than before. If certain assumptions about past hydrologic relations can be met, particle-size analysis is a way of comparing the histories of geographically very different lakes, including lakes from tropical, temperate, and arctic regions.
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    Hydrobiologia 103 (1983), S. 217-224 
    ISSN: 1573-5117
    Keywords: paleolimnology ; Greenland ; Holocene ; oligotrophication ; pollen analysis ; macrofossils ; arctic
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    Notes: Abstract During the Holocene most West Greenland lakes passed from an early eutrophic stage, rich in both flora and fauna, through a mesotrophic to an oligotrophic stage with very low productivity. Temperature conditions were limiting factors only in the very beginning, whereas chemical factors alone were decisive later on.
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    Hydrobiologia 103 (1983), S. 205-210 
    ISSN: 1573-5117
    Keywords: paleolimnology ; colluvium ; soil chemistry ; clay ; phosphorus loading ; tropical lakes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The long-term impact of Maya culture on a lowland tropical watershed is assessed, using data from a 9.2 m sediment core taken from deep water (28 m) in Lake Quexil. Human population growth, estimated by the 1980 archaeological survey, is associated with a shift in the composition of the sediment to a dominance by inorganic material, the Maya clay formation, beginning ca. 3500 B.P. Increasing settlement densities are correlated with accelerated influxes of phosphorus, carbonates, and siliceous sediment. However, chemical data do not track short-term population fluctuations closely. Because much of the sediment is delivered as colluvium, and not by running water, there is a lag between terrestrial disturbance and impact on the aquatic system. As an indication of this lag, contemporary high sedimentation rates are a residual of Maya activity that virtually ceased some 300–400 years B.P. Comparison of the deep-water core with a shallow-water (7 m) section, based on palynological correlation, reveals only minor differences in proximate chemical composition. Chemical influxes are much higher at the deep-water site, however, as a consequence of sediment focusing in this hyperconical basin. Chemical analyses of soil samples from 21 test pits in the Quexil basin support the principal conclusion that bulk soil movement was the mode of nutrient transfer to the lake, following forest clearance by the Maya.
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    Hydrobiologia 103 (1983), S. 211-216 
    ISSN: 1573-5117
    Keywords: paleolimnology ; Pleistocene aridity ; calcite ; dolomite ; gypsum ; laminated gyttja
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    Topics: Biology
    Notes: Abstract The transition from an arid, glacial Late Pleistocene to an early Holocene (Gamblian ‘pluvial’) moist period has not been documented in Central America. Finding accessible volcanic lakes too youthful, and knowing that most Florida and Yucatan lakes were dry during glacial ages, we sought appropriate deposits lying deeper than 40 m in the deeper karst lakes of the Peten, in northern Guatemala. The bottom half of a 19.7 m core from Lake Quexil, and the bottom third of a 15 m core from Lake Salpeten, appear to be of Pleistocene age. The sediments contain lacustrine shells, sponge spicules, and Pinus pollen, and include several bands of humified gyttja with fragments of wood, but are dominantly montmorillonitic and mixed-layer clays and may be in part colluvial, like the later Holocene Maya clay. Calcite, gypsum, and (in presently saline Lake Salpeten only) dolomite indicate shallow, closed, moderately saline lakes 30–40 m lower than at present. In both cores a layer of inorganic sediment with gypsum dominant, perhaps recording the most arid phase of the glacial Late Pleistocene, overlies a similar clay layer with calcite dominant. As calcite and dolomite occur throughout the section(s), both minerals are believed to be detrital, but one source of calcite is algal crusts, formed and exposed today in the littoral and supralittoral zones. The early Holocene rise of lake levels formed several meters of fossiliferous gyttja with pollen of mesic tropical forest, now assigned to the pan-tropical Gamblian moist episode. Where deposited in oligomictic or meromictic lakes 〉 30 m deep, Gamblian gyttja of pollen zone Pl is finely laminated, the dark layers being richer in Ptot and Stot and poorer in Fe, Mn, Mg, and K than the light (clay) layers, but we cannot yet say that the laminae are annual.
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    Hydrobiologia 103 (1983), S. 225-230 
    ISSN: 1573-5117
    Keywords: paleolimnology ; pollen analysis ; sediment accumulation rate ; vegetation history ; Holstein ; Postglacial
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    Notes: Abstract Pollen analysis was carried out on the sediments of 10 lakes in eastern Holstein. Sediment accumulation rates varied considerably both from lake to lake and also between respective periods. Increases in sediment accumulation rate often coincide with the Neolithic settlement periods. Faunal remains have been examined from two of these lakes (cf. Günther 1983; Hofmann 1983).
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    Hydrobiologia 103 (1983), S. 235-239 
    ISSN: 1573-5117
    Keywords: paleolimnology ; cladoceran analysis ; chironomid analysis ; successions ; lake development
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    Topics: Biology
    Notes: Abstract In the subfossil assemblages of chironomids and cladocerans significant changes were found during the late-glacial and postglacial development of a small, shallow North German lake. The successions observed were obviously not related to eutrophication but to factors such as climatic conditions and lake siltation. The absence of species of the subgenus Eubosmina during postglacial times as well as the predominance of Alonella species among the chydorids was due to the small dimensions of the water body.
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    Hydrobiologia 103 (1983), S. 231-234 
    ISSN: 1573-5117
    Keywords: paleolimnology ; lake development ; trophic status ; Chironomidae ; Cladocera ; Ostracoda
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Microfossil analysis was carried out on 90 samples of a sediment core from Grossensee. The diversity of subfossil Cladocera, Chironomidae, Chaoborus, and Ostracoda reflects at least five different stages of development. Three extended cycles correspond to different climatic periods in the first 10 000 years of the lake's history, while during the last 2 000 years, two shorter cycles can be identified which correlate with proofs or even historical events for human activity in the catchment area. During this time the profundal fauna changed from stenoxibiontic to euryoxibiontic species. In the planktonic fauna a succession of three Eubosmina types, B. longispina, B. coregoni kessleri, and B. coregoni coregoni, was observed. The chydorid and ostracod assembly of the littoral showed no clear change.
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    Hydrobiologia 103 (1983), S. 261-263 
    ISSN: 1573-5117
    Keywords: paleolimnology ; diatoms ; eutrophication ; Ladoga
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    Topics: Biology
    Notes: Abstract Combined paleolimnological investigations of a 1.8 m sediment core from the deepest north-western part of Lake Ladoga show stratigraphic changes in granulometric and chemical composition, organic matter content, diatom species composition and chlorophyll ‘a’ concentration. The sediment accumulation rate was calculated and 5 stages of lake history over the last 4 000 years were described. Changes in lake environment were mainly caused by changes in climate and lake water balance. Human impact on the lake was also traced.
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    Hydrobiologia 103 (1983), S. 265-268 
    ISSN: 1573-5117
    Keywords: paleolimnology ; diatoms ; plankton ; eutrophication ; sediment accumulation rate
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    Topics: Biology
    Notes: Abstract Species composition, quantity and distribution of diatoms in both the plankton and the surface sediments (0–30 cm) of mesotrophic Lake Krasnoye and eutrophic Lake Vishnevskoye (Karelian Isthmus) were studied. In the mesotrophic lake the composition of dominant diatoms (mainly Melosira) corresponded to those in the plankton. The Araphidineae/Centrales (A/C) ratio was 0.2–0.3%, increasing in the upper layers to 7%. Alkaliphilous and alkalibiontic forms constituted 60% of the total. In the eutrophic lake diatoms with thin valves (mainly Synedra) predominated in the plankton but their quantity in the sediments was insignificant in comparison with other plankton. Nevertheless, the A/C ratio was much higher, 17–35%. Alkaliphilous and alkalibiontic forms accounted for 78–90% of the total number of valves. In both lakes the highest number of diatom valves was registered in the upper layer of the sediments. From the ratio of the total number of diatoms in the upper 5 cm layer to their annual flux to the sediment from the plankton the approximate sediment accumulation rate was calculated to be 1.9 mm a−1 for the mesotrophic lake and 2.5 mm a−1 for the eutrophic one.
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    Hydrobiologia 103 (1983), S. 275-279 
    ISSN: 1573-5117
    Keywords: paleolimnology ; freshwater diatoms ; Middle-Valdaj
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This paper presents results of diatom analyses of dated Upper-Pleistocene lacustrine deposits from the Shapkina river in the Bolshezemelskaja tundra of the USSR. Both diatom systematics and palaeoecology have been studied. A marine diatom assemblage was found in the lower part of the sedimentary sequence and a rich freshwater assemblage, including more than 200 taxa, was found in the upper part. Some of the characteristic diatoms are typical of standing or slowly-moving waters of more southern and western districts but are not found in the Bolshezemelskaja tundra lakes today. According to 14C dating, the age of the deposit is about 40 000 years, and represents the Middle-Valdaj (Middle-Weichselian). Palynological and diatom analysis suggest that the climate was similar to the present, that of an interglacial.
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    Hydrobiologia 103 (1983), S. 269-273 
    ISSN: 1573-5117
    Keywords: paleolimnology ; palynology ; pollen diagram ; Holocene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In the last decade palynological investigations of the sediments of large lakes in the USSR (Ladoga, Onega, Kubenskoye, Vozhe, Lacha, Beloye) and the Baltic Sea have been carried out. Limnic and marine sediments are notable for their high pollen and spore concentrations and pollen diagrams from these deposits have a regional significance. The diagrams from Lakes Onega and Ladoga belong to the Kola-Karelian type while the sediments of Lacha, Vozhe, Kubenskoye and Beloye belong to the North-Russian type (Neustadt 1957). Three types of Holocene pollen diagrams are registered for the Baltic Sea. Modern and fossil pollen spectra reflect the composition of the zonal vegetation. Late-glacial sediments are found in several profiles of lake and sea deposits. Holocene deposits are divided into eight stratigraphic zones: Late-glacial sediments (DR2-Al-DR3) and Post-glacial sediments (PB, BO, AT, SB, SA). The zonations of lake and sea sediments can be clearly correlated.
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  • 53
    ISSN: 1573-5117
    Keywords: paleolimnology ; pollen analysis ; land drainage ; sediment accumulation ; 210Pb dating ; 14C reservoir effect
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Previous measurements of the 14C activity of lake sediments and Potamogeton plants from Säynäjälampi, collected in 1970, indicated a severe hard-water effect (Donner et al. 1971). Samples from new cores, collected in 1977, were sliced into thin sections down to 18 cm. The 14C concentration in samples down to 7 cm was similar to that obtained by the Helsinki Laboratory. The corresponding apparent 14C age is almost 2 000 years. Light-coloured layers, apparently due to catchment drainage, were used together with density diagrams to correlate the cores. The drainage history gives a time-scale showing that the lowest samples dated to correlate the cores. The drainage history gives a time-scale showing that the lowest samples dated were just old enough to be pre-bomb sediments. Three samples of submerged plants were also examined, indicating some exchange with the air and an appreciable reservoir age. The activity of the plants was significantly lower in 1978 than in 1970. One sample, well below the lowest drainage stratum, indicates a reservoir age of ca. 1 100 years. This sample yielded an apparent age of the INS fraction which was significantly different from that of the SOL fraction. The difference was determined to ca. 600 years. Total 210Pb was determined via 210Po using isotope dilution while 226Ra was measured with the help of radon emanation. The 226Ra was used to estimate the supported 210Pb. The unsupported 210Pb showed a higher sediment accumulation rate for the top nine cm of one core (the length investigated was 16.5 cm). The varying accumulation rate was explained by human activities, as could be seen from the heterogenous sediment composition of the cores. Recent vegetational change is described from direct observations since 1957. Pollen analysis does not give any reliable indication of agriculture.
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    Hydrobiologia 103 (1983), S. 29-35 
    ISSN: 1573-5117
    Keywords: paleolimnology ; 210Pb ; sediment accumulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The last few years have seen a dramatic growth in the use of 210Pb sediment dating. Despite this, considerable doubt still surrounds the nature of the processes by which 210Pb is deposited in lake sediments, and this has lead to a situation where there is a choice of dating models offering different interpretations of 210Pb data. In assessing 210Pb data it is therefore essential to first of all determine whether data is consistent with the assumptions of the dating model, and to then compare the 210Pb chronology with independent dating evidence. We have tested 210Pb data from a wide variety of sites, and our calculations indicate that the crs (constant rate of 210Pb supply) model provides a reasonably accurate chronology when the total 210Pb contents of cores from neighbouring locations are comparable.
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  • 55
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    Hydrobiologia 106 (1983), S. 43-57 
    ISSN: 1573-5117
    Keywords: eutrophication ; paleolimnology ; diatoms ; sediment chemistry ; peatland management
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Stratigraphy of diatoms and chemistry in the surface sediment deposited at 35 m depth in Lake Polvijärvi was studied. The existence of annual laminations or varves in the sediment allowed a precise dating of the profile. Diatoms were analysed in 0.5 cm sequences; from 0 to 16.0 cm continuously and then intermittently every fourth 0.5 cm down to 44.0 cm. Sediment chemistry (loss-on-ignition, C, N, Fe, Mn, Mg, P, chlorophyll and carotenoids) was analysed from sediment surface down to 10.5 cm of altogether 33 subsamples, each containing 1–3 varves, and spanning the period 1921–1980. From 4.5 cm depth upwards the diatom concentration strongly increases, and the plankton diatom succession from Tabellaria flocculosa through Asterionella formosa to Melosira ambigua and Fragilaria crotonensis reflects a marked eutrophication of the lake. This algal succession occurs in pace with an increase in sediment accumulation rate and changes in sediment chemistry, which indicate increased allochthonous inputs and enhanced algal production in the lake. The change of the lake ecosystem is contemporaneous with extensive peatland draining and fertilizing that was carried out on its watershed during the past two decades. Existing chemical data from a number of lakes situated within the drainage area prove that at present the treated peatlands are the main source of nutrient loading of Lake Polvijärvi. A former period with indications of slightly increased productivity of the lake was dated by varve counting to AD 1690–1910 (35–12 cm). This period (characterised by Asterionella formosa) may coincide with that of the slash-and-burn cultivation in the area.
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  • 56
    ISSN: 1432-0878
    Keywords: Nucleus lateralis tuberis ; Hypothalamo-hypophysial relationship ; Immunocytochemistry ; Prolactin cells ; Teleosts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the rainbow trout the pars lateralis is the most prominent part of the nucleus lateralis tuberis (NLT). To demonstrate a morphological relationship between this lateral part of the NLT and the pituitary, immunocytochemistry was applied as a staining method. Experiments were carried out on glutaraldehyde-picric acid-acetic acidfixed brain sections of mature male and female rainbow trout using the peroxidase-anti-peroxidase immune technique with an antiserum against 27-S-methylglucagon as the first antibody. Most of the cells in the NLT/pars lateralis reacted with the antiserum. Axons from these cells enter the pituitary, extending exclusively in the numerous neurohypophysial digitations in the pars intermedia. No immunoreactive neurohypophysial protrusions were found in those parts of the adenohypophysis where the gonadotropic cells are located, indicating that the lateral part of the NLT is not directly involved in the control of gonadotropin secretion. In addition to cells of the NLT/pars lateralis only prolactin cells in the rostral pars distalis of the adenohypophysis reacted with the antiserum used.
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  • 57
    ISSN: 1432-0878
    Keywords: Villin ; Intestinal brush border ; Contractile proteins ; Immunocytochemistry ; Immunostaining of blotted gels
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Villin, a 95,000 dalton polypeptide of intestinal brush border which is known to bundle or sever actin filaments in a Ca++-dependent manner, was localized in rat and chicken intestinal epithelium by means of immunocytochemistry at the light- and electron-microscopic levels. Specific antibodies to villin were raised in rabbits immunized with villin purified from chicken intestinal epithelium. Anti-villin bound selectively to the microvillus filament bundle from its tip down to the rootlets. These findings indicate that the well-known stability of rootlet filaments towards elevated Ca++ ion concentrations cannot be explained by the absence of villin. Therefore additional factors must exist which prevent the rootlets from Ca++-villin mediated disassembly.
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  • 58
    ISSN: 1432-0878
    Keywords: TRH ; Immunocytochemistry ; Autoradiography ; Arcuate nucleus ; Median eminence ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Immunocytochemical and autoradiographic localization of thyrotropin-releasing hormone (TRH)-and 3H-TRH-binding sites was studied in the arcuate nucleus-median eminence region of the rat. TRH-like immunoreactivity was found in dense granular vesicles (90–140 nm in diameter) in TRH-like immunoreactive nerve fibers and terminals. In the median eminence, the immunoreactive terminals were observed to be in direct contact with the perivascular basal lamina of the portal vessel and to form synaptoid contacts with tanycytes. In the arcuate nucleus, the immunoreactive terminals were often found to form axosomatic and axo-axonic, and/or axo-dendritic synapses. The uptake of tritiated TRH into the nerve fibers and terminals of the median eminence was also observed by autoradiography and the distribution and localization of silver grains in them were analyzed quantitatively by circle analysis. Thirty minutes after intraventricular infusion of 3H-TRH, radioactive labeling occurred in type-2 and 3-nerve fibers and terminals containing dense granular vesicles in the median eminence. It is therefore suggested that the neurons labeled after 3H-TRH infusion possess certain functions as physiological recognition sites or receptors for TRH.
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  • 59
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    Cell & tissue research 228 (1983), S. 525-534 
    ISSN: 1432-0878
    Keywords: Immunocytochemistry ; Vasopressin ; Oxytocin ; Bed nucleus, stria terminalis ; Lateral septum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the dorsal and ventral portions of the bed nucleus of the stria terminalis of the rat numerous cell bodies immunoreactive for vasopressin and neurophysin II were found after colchicin pretreatment. These cells are predominantly multipolar but sometimes also bipolar, and have a width and length of approximately 9 and 16 μm, respectively. In the homozygous Brattleboro rat, which is deficient in vasopressin, no immunoreactive vasopressin was found in these cells. Following incubation with anti-oxytocin and anti-bovine neurophysin I, only magnocellular immunoreactive cell bodies were found in the septal region. The consequences of these results concerning the vasopressin fiber pathways in the brain are discussed.
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  • 60
    ISSN: 1432-0878
    Keywords: Immunocytochemistry ; Uteroglobin ; Rabbit ; Epididymis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Using the immunoperoxidase technique and specific goat antiserum to uteroglobin, selective immunochemical staining products are localized in the epididymal epithelium of the caput and proximal corpus region, at the adluminal border of the cauda epididymidis and, as well known, in epithelial cells of the endometrium of pregnant and progesterone-treated rabbits. Specific staining is also seen on spermatozoa. A uteroglobin-like antigen has been similarly localized in alveolar and bronchial epithelial cells of the lung. Testis, prostate, seminal vesicle and ductus deferens do not seem to contain in their tissues immunoreactive uteroglobin-like antigens. Similarly, the uterus and ductus epididymidis of immature rabbits are devoid of immunoreactivity. The presence of uteroglobin-like antigens in tissues other than the endometrium, particularly the ductus epididymidis, stimulates new discussions on the function of this protein in reproductive physiology and fertility research.
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  • 61
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    Cell & tissue research 228 (1983), S. 265-276 
    ISSN: 1432-0878
    Keywords: Met- and leu-enkephalin ; Immunocytochemistry ; Ultrastructure ; Lateral septum ; Guinea-pig brain
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary By the use of antisera to met-enkephalin and leu-enkephalin, enkephalin-containing structures were visualized in the lateral septum of the guinea-pig brain. The present results do not reveal immunoreactive perikarya in this area. The immunostaining is exclusively located in numerous nerve fibers and endings mostly encompassing neuronal perikarya, which accounts for the fact that at the light-microscopic level cellular somata appear to be immunostained. The immunoreactive terminals and fibers contain granules approximately 110nm in diameter and synaptic vesicles. The origin and the functional role of these numerous enkephalin terminals remain to be established. This work was supported by a grant from INSERM N∘ 804012
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  • 62
    ISSN: 1432-0878
    Keywords: Thyroid ; Pituitary ; Thyrotrops ; Gonadotrops ; Immunocytochemistry ; Teleosts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Alcian blue and periodic acid-Schiff-positive “granular basophils” with electron-dense granules ±160 nm in diameter, and weakly developed irregular cisternae of the granular endoplasmic reticulum (GER) are present in the rostral pars distalis (RPD) and the proximal pars distalis (PPD) of immature rainbow trout. They all react with antisalmon αβ-gonadotropin. However, only granular basophils in the caudal RPD and the rostro-dorsal PPD, often bordering on and sometimes surrounded by neurohypophysial tissue, react with anti-human β-TSH. These cells, considered as the source of thyrotropin, show degranulation and dilatation of the GER-cisternae in fish treated with potassium perchlorate. The thyroids of the goitrogen-treated animals had relatively numerous small follicles with a high epithelium. The remaining granular basophils are gonadotrops.
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  • 63
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    Cell & tissue research 231 (1983), S. 205-213 
    ISSN: 1432-0878
    Keywords: Immunocytochemistry ; Insulin ; Glucagon ; Pancreatic polypeptide ; Diabetes mellitus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Rats rendered diabetic by streptozotocin were subjected to pancreas transplantation. After twenty weeks, the duct-ligated pancreas transplant was studied morphometrically to determine the effect of duct occlusion on the various cell populations of the islets. Concomitantly, the streptozotocin-treated host pancreas was examined for a possible influence of the graft on the diabetic pattern of islet cell population. Twenty weeks after pancreas transplantation, the volume fractions of insulin, glucagon, somatostatin and pancreatic polypeptide cells in the graft islets did not differ from those of the normal control pancreas. In the pancreas of nontransplanted diabetic rats, insulin-positive B cells were reduced from 60–65% to less than 10% of the islet volume, whereas non-B cells were significantly increased in volume density. The changes in fractional volume of the various islet cells correlated fairly well with changes in plasma concentration of the corresponding pancreas hormones. In the recipient's own pancreas, the relative volumes of glucagon and somatostatin cells were unaffected by the pancreas transplant. However, the insulin cell mass was significantly increased, and comprised about 20% of the islet volume, while cells containing pancreatic polypeptide were found only sporadically.
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  • 64
    ISSN: 1432-0878
    Keywords: Somatotrops ; Oncorhynchus ; Immunocytochemistry ; Immunodiffusion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Pituitary somatotrops were localized in four species from the genus Oncorhynchus using an antiserum to chum salmon (Oncorhynchus keta) growth hormone. The antiserum cross-reacted specifically with the acidophils in the caudal pars distalis of all species tested. Corroborative immunodiffusion studies were also conducted with pituitary homogenates from the same species. Immunological identity of the GH molecule was observed for all species tested.
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  • 65
    ISSN: 1432-0878
    Keywords: Immunocytochemistry ; Human TSH β subunit ; Teleosts ; TSH cells
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A rabbit antiserum raised against human thyrotropin (TSH) β subunit (anti-human β-TSH) was used to identify TSH cells in the pituitaries of five species of teleosts by means of the unlabeled antibody peroxidase-antiperoxidase (PAP) technique. The anti-human β-TSH reacted only with polygonal basophils located in the rostral pars distalis (RPD) in the pituitaries of sexually immature eel (Anguilla japonica). In the pituitaries of medaka (Oryzias latipes) and white-edged rockfish (Sebastes taczanowskii), this serum reacted specifically with only one cell-type located in the antero-dorsal proximal pars distalis (PPD) and antero-ventral PPD, respectively. In the pituitaries of two species of salmonids, sexually immature rainbow trout (Salmo gairdneri) and sexually mature chum salmon (Oncorhynchus keta), the anti-human β-TSH bound to the polygonal basophils in the ventral PPD adjacent to the RPD. Thus, the present immunocytochemical study identified polygonal basophils located in the RPD or PPD as TSH cells in five species of teleosts.
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  • 66
    ISSN: 1432-0878
    Keywords: Pituitary gland ; S-100 protein ; Castration ; Thyroidectomy ; Postnatal development ; Immunocytochemistry ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Pituitary folliculo-stellate cells and associated cells were studied immunohistochemically throughout the life of male and female rats. The marginal layer cells of the pars distalis and intermedia as well as the folliculo-stellate cells were immunostained with anti-S 100 protein serum. The immunostained folliculo-stellate cells in one section were identified as themselves by their ultrastructural properties on the adjacent section. Corticotrophs, thyrotrophs and prolactin cells, characterized by the stellate shape, were not immunostained with anti-S 100 protein serum. Reactivity for S-100 protein appeared on postnatal Day 6 in the marginal layer cells of the pars intermedia, and appeared on Day 10 in those cells of the pars distalis. No immunostained folliculostellate cells appeared before Day 6, but a few of them appeared on Day 10. Thereafter they increased in number, having more intense immunostaining with advancing age. In castrated rats, the immunostained folliculo-stellate cells appeared quite numerously, with branched cytoplasmic processes surrounding the gonadotrophs. In the thyroidectomized rats, however, folliculo-stellate cells lacked ramified cytoplasmic processes, and their topographic affinity for thyrotrophs was negligible.
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    Cell & tissue research 234 (1983), S. 427-437 
    ISSN: 1432-0878
    Keywords: Corticotropes ; Rat fetus ; Ultrastructure ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Corticotropes of rat fetuses aged 16, 18 and 21 days were localized by the indirect antibody-enzyme method on semithin sections of the pituitary. The development of the ultrastructure of these cells was observed on consecutive ultrathin sections. In comparison with previous data our present results show that identification of a fetal cell type cannot be based entirely on morphological criteria. The structural peculiarities of corticotropes obtained from studies in vivo are compared with those observed in cells maintained in vitro.
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  • 68
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    Cell & tissue research 234 (1983), S. 125-134 
    ISSN: 1432-0878
    Keywords: Vasopressin ; Immunocytochemistry ; Electron microscopy ; Hypothalamus ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Vasopressin-containing neurons, identified by immunocytochemistry, are located predominantly in the posterior magnocellular division of the paraventricular nucleus of the rat hypothalamus. By electron microscopy, the immunoreaction product is seen within the cell bodies and neuronal processes. In the perikarya and dendritic processes, the immunoreactive material is associated primarily with neurosecretory granules. Axonal processes, identified by their content of microtubules and accumulation of neurosecretory granules, show the immunoreaction product in association with both of these organelles. Afferent axo-dendritic, axo-somatic and putative axo-axonic synapses with immunostained vasopressinergic neurons can be identified. The presynaptic profiles do not contain immunoreactive material. This study contributes to the ultrastructural characterization of vasopressinergic neurons in the paraventricular nucleus and of their afferent synaptic input.
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  • 69
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    Cell & tissue research 234 (1983), S. 319-333 
    ISSN: 1432-0878
    Keywords: Serotonin ; Goldfish ; Immunocytochemistry ; Central nervous system
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The distribution of immunoreactive serotonin (5-HT) was investigated in the brain and pituitary gland of the goldfish with the use of indirect immunofluorescence technique. Immunoreactive cerebrospinal fluid-contacting neurons were found in the nucleus recessus lateralis and in the nucleus recessus posterions of the hypothalamus. Additional immunoreactive neurons were observed ventro-lateral to the posterior commissure in the nucleus dorsolateralis thalami. This group of cell bodies extends posteriorly as far as the rostral midbrain tegmentum. At the level of the isthmus, numerous immunoreactive cell bodies were located medially between the fiber bundles of the fasciculus longitudinalis medialis. Finally, a few isolated immunoreactive cells were observed in the medulla oblongata. In the pituitary gland, immunoreactive fibers and cells were found primarily in the pars distalis. The origin of the numerous fibers of the telencephalon is not clear, however, they may arise from the perikarya located in the raphe region. The general organization of the ascending and descending 5-HT-systems of the teleost brain appears to be similar to that described in mammals.
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  • 70
    ISSN: 1432-0878
    Keywords: Immunocytochemistry ; Ontogeny ; LHRH ; Brain ; Teleost fish
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary We have traced the development from birth to puberty of the ir-LHRH producing centers, the nucleus olfactoretinalis (NOR), nucleus praeopticus periventricularis (NPP) and nucleus lateralis tuberis pars posterior (NLT), in male sibling platyfish genetically determined by differing P alleles to mature at two different ages. In sexually immature fish of both genotypes ir-LHRH is first localized in the NOR in perikarya and neuronal fibers and in the NPP where it is limited to the fibers. At the onset of puberty in early maturing fish (11 weeks), ir-LHRH accumulates in perikarya and fibers of the NPP and NLT. Late maturing siblings first have ir-perikarya in the NPP and a few ir-fibers in the NLT at the same developmental stage but at a later age (25–26 weeks). Ir-perikarya are still not seen in the NLT in late-maturing fish even when maturation is complete. This pattern of a sequential development of the three ir-LHRH containing areas of the brain, which we refer to as a “cascade effect”, is similar in early and late genotypes. It is directly correlated to stage of development and not to chronological age. It is clear that the appearance of LHRH producing brain centers precedes, and is presumably essential for, the completion of the development of pituitary gonadotrops and the subsequent maturation of the gonads. The functional significance of the NOR in puberty and reproduction and as a possible site of action for the P gene is discussed.
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  • 71
    ISSN: 1432-0878
    Keywords: Adipokinetic hormone ; Locusta migratoria ; Neurohormone ; Peptidergic neuron ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary An antiserum was raised against tyrosine-adipokinetic hormone ([Tyr1]-AKH). In immunohistochemical procedures, it revealed the AKH cells in the glandular lobes of the corpora cardiaca (CC) of Locusta migratoria with high specificity. In addition, an immunologically related peptide was detected in certain neurons of the central nervous system which suggests that this AKH-like peptide may have a neurotransmitter function. The glandular lobes contain immunoreactive AKH cells in all postembryonic stages, and no essential differences in morphology and distribution of the cells in nymphs and adults were seen. The amount of AKH, stored predominantly in the cell projections, differs widely among cells and individuals. The brain of adults and nymphs contains several small populations of intensely stained neurons. In last-instar and adult specimens, each half contains 10–12 “normal”-sized neurons in the protocerebrum (including the optic lobe) and deutocerebrum, and in addition 15–18 small reactive neurons. Their axons and numerous branchings traverse the neuropile of proto-, deuto-, and tritocerebrum, except for the pedunculate bodies and antennal lobes. Some of the axons run into the storage lobe of the CC; it is unknown if their content is released into the haemolymph. Other axons run into the ganglia of the stomatogastric nervous system and into the circumoesophageal connectives. The suboesophageal ganglion also contains 8 immunoreactive neurons. It is unknown to which extent the immunoreactive substances in glandular and nervous tissue are chemically and physiologically related.
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  • 72
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    Cell & tissue research 233 (1983), S. 69-80 
    ISSN: 1432-0878
    Keywords: Somatostatin ; Hypothalamus ; Immunocytochemistry ; Human ; Rhesus monkey
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Immunocytochemical methods were used to compare the localization of somatostatin (SRIF) in the human and rhesus monkey hypothalamus. The distribution of SRIF-containing cell bodies and fibers is similar in the two species. Perikarya are located predominantly in the periventricular region and to a lesser extent in the ventromedial nucleus. Fibers occur in dense clusters within the periventricular region, ventromedial nucleus, arcuate nucleus, median eminence, and pericommissural area of both species. Analysis of serial sections suggests that fibers originate from cells in the periventricular region, extend ventrally through the ventromedial and arcuate nuclei to terminate around the portal vessels of the infundibular stalk, and thereby participate in the regulation of anterior pituitary function. Somatostatinergic fibers are also found surrounding non-immunoreactive perikarya in the ventromedial nucleus and periventricular region of both primates. This arrangement may support somatostatin's postulated role as a neurotransmitter or neuromodulator. The strong similarity between the localization of hypothalamic SRIF in the human and rhesus monkey supports the use of the rhesus monkey as a model for the study of somatostatin as a neuroendocrine regulator in the human.
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  • 73
    ISSN: 1432-0878
    Keywords: Immunocytochemistry ; Vasopressin ; Dorsomedial hypothalamus ; Amygdala ; Locus coeruleus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Recently, the existence of a vasopressin-immunoreactive cell group was described in the bed nucleus of the stria terminalis (van Leeuwen and Caffé 1983). In the present investigation additional nuclei containing vasopressin-immunoreactive cells were found, after colchicine pretreatment, in the dorsomedial hypothalamus, medial amygdaloid nucleus and the locus coeruleus. Vasopressin-immunoreactive cells in the dorsomedial hypothalamus and medial amygdaloid nucleus are small (8–14 μm and 10–14 μm, respectively), while those in the locus coeruleus are medium-sized (20–25 μm). Incubation with anti-bovine neurophysin II and anti-rat neurophysin revealed staining of the same cell group in the above-mentioned areas. None of these cell groups show stained cells after incubation with anti-oxytocin and anti-bovine neurophysin I. When sections of the homozygous Brattleboro rat, which shows a deficiency in vasopressin synthesis, are incubated with anti-vasopressin, anti-bovine neurophysin II, or anti-rat neurophysin, no immunoreactivity can be observed in these brain regions. The above-mentioned cell groups may contribute to the vasopressinergic innervation of brain sites that have been reported to persist after lesioning of the suprachiasmatic, paraventricular and bed nuclei of the stria terminalis.
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    Cell & tissue research 233 (1983), S. 233-236 
    ISSN: 1432-0878
    Keywords: Y-organ ; Steroid secretion ; Ecdysone ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Ecdysone was demonstrated by ultrastructural immunocytochemistry to be present in the mitochondria of the Y-organs of the crayfish Orconectes limosus. This is in remarkable contrast to the prothoracic glands of insects and suggests substantial differences in the biosynthesis of the same hormone, ecdysone, in crustaceans and insects.
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  • 75
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    Cell & tissue research 233 (1983), S. 99-111 
    ISSN: 1432-0878
    Keywords: Neurosecretion ; Vasopression ; Osmotic stress ; RIA ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A correlative radioimmunoassay (RIA) and immunocytochemical (ICC) study was carried out on vasopressin (VP) distribution and content in brains of normal and 3-day water-deprived rats. By RIA there were statistically significant differences in brain VP per pg/mg between normal and osmotically stressed specimens in hypothalamus (338.4 versus 134.4), thalamus (4.8 versus 0.9), septum (18.0 versus 3.4), striatum (1.6 versus 0.7) and amygdala (17.3 versus 1.3), but not in other brain regions measured. Pituitary VP decreased from 71.1 to 8.7 ng/mg, and plasma VP rose from 3.6 to 19.3 pg/ml during water deprivation. Application of the peroxidase-anti-peroxidase ICC method of Sternberger to vibratome sections showed that VP-immunoreactivity in dehydrated specimens decreased in perikarya of paraventricular nucleus and suprachiasmatic nucleus, while intrahypothalamic immunoreactive magnocellular fibers appeared more conspicuous due to proliferation of large Herring bodies. In extrahypothalamic sites VP-immunoreactivity in water-deprived rats was visibly reduced in periventricular thalamus and septum. Thus it is apparent that both intra- and extrahypothalamic VP are affected by osmotic stress, and these results are discussed within the context of current ideas relating to co-activation of neurosecretory cells that project to different sites.
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  • 76
    ISSN: 1432-0878
    Keywords: Lung development ; Fibronectin ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The development of the rat lung is a process of continuing morphological change. Indications from work in other mammalian systems suggest that fibronectin may be important in the control of this process. The present study has examined embryonic, neonatal, and adult lung tissue of the rat by means of the peroxidase-antiperoxidase (PAP) technique to demonstrate fibronectin at the light-microscopic level. Positive reaction was observed with anti-fibronectin serum in all stages examined. Control sections treated with pre-immune serum or no primary serum gave negative results in each case. Fibronectin in adult tissue was localized to the alveolar surface and alveolar basal lamina. Neonatal tissue showed fibronectin on pulmonary tubule walls and in basal lamina while embryonic tissue revealed localization of the protein in the basal lamina and in association with small groups of cells at the base of septal buds. These findings suggest a role for fibronectin in the control of rat lung development. The results are discussed in terms of the known functions of fibronectin as a preliminary matrix for the subsequent deposition of collagenous connective tissue, as a cellular adhesion protein, and as surface-bound material for cellular migration.
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    Cell & tissue research 233 (1983), S. 389-402 
    ISSN: 1432-0878
    Keywords: Calcium-binding protein (CaBP) ; Immunocytochemistry ; Rat cerebellum ; Development ; Vitamin D
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Specific antibodies raised against a human 28 000 dalton cerebellar calcium-binding protein (CaBP) were used in an immunocytochemical study during development of the rat cerebellum. Both light and electron microscopy showed (1) that labelling was entirely restricted to the Purkinje cells, (2) that it appeared very early in Purkinje cell development, (3) that the entire cell was labelled from the tip of the smallest dendrites to the axonal terminals, and (4) that with increasing age, the immunoreaction appeared to be progressively restricted to the cell and organelle membranes.
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  • 78
    ISSN: 1432-0878
    Keywords: α-Endorphin ; Immunocytochemistry ; Median neurosecretory cells ; Peptidergic neurones ; Calliphora vomitoria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A group of the 24–26 paraldehyde fuchsin-positive median neurosecretory cells (MNC) in the pars intercerebralis of the brain of the blowfly, Calliphora vomitoria, has shown immunoreactivity towards three different antibodies to α-endorphin, a peptide that corresponds to the amino acid sequence present between residues 61 and 76 of the precursor molecule, β-lipotropin (β-LPH). The immunoreactive material could be followed in axons within the median bundle, the tract through which neurosecretory material from the MNC is passed down to the corpus cardiacum (CC). The α-endorphin-immunoreactive material was observed leaving the CC in the cardiac-recurrent nerve, dorsal to the proventriculus, in the direction of the abdomen. The cells that contain the α-endorphin-like material are different from those of the MNC that contain insulin-, pancreatic polypeptide-, and gastrin/CCK-like peptides. This finding demonstrates the considerable complexity and peptidergic nature of the MNC and constitutes further evidence that morphinomimetic-like peptides are present in the nervous system of invertebrates.
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  • 79
    ISSN: 1432-0878
    Keywords: Pars intermedia ; Oxytocin ; Dopamine ; Serotonin ; Immunocytochemistry ; Rabbit, hare
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The pars intermedia of the pituitary in the rabbit and hare is abundantly innervated by axons reacting selectively with antibodies against oxytocin. These axons contain dense secretory vesicles about 140 nm in diameter, i.e., smaller than those in the neurosecretory axons of the neural lobe. No fiber elements staining for other peptides (vasopressin, somatostatin, substance P) were observed in the pars intermedia, except rare leu-enkephalin axons restricted to the rostral zone of the gland. Dopaminergic innervation appears to be completely absent from the intermediate lobe. This was shown by the lack of reaction with an antibody against tyrosine-hydroxylase, which did reveal a well-developed tubero-infundibular system of nerve fibers. Axons reacting with an antibody against serotonin were irregularly distributed in the pars intermedia. In the absence of dopaminergic axons, the extensive oxytocin-like innervation may play a major role in regulating the melanotrophic cell activity in the Leporidae.
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  • 80
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    Cell & tissue research 229 (1983), S. 541-550 
    ISSN: 1432-0878
    Keywords: Hypothalamus ; Hagfish ; Somatostatin neurons ; Vasotocin neurons ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The brain of the Pacific hagfish, Eptatretus stouti, was studied immunocytochemically using antisera against somatostatin (SRIH), arginine vasopressin (AVP), and adrenocorticotropic hormone (ACTH). SRIH-immunoreactive perikarya were distributed bilaterally in the postoptic nucleus and in the hypothalamic nucleus. Although several short, stained fibers were observed in the vicinity of the perikarya, SRIH-immunoreactivity was not found in the neurohypophysis, nor in other parts of the brain. On the other hand, presumed arginine vasotocin (AVT) perikarya were distributed in an arc-shaped region extending from the posterior part of the preoptic nucleus to the anterior-most end of the hypothalamic nucleus and projected their fibers to the neurohypophysis. Most presumptive AVT perikarya were located close to the paired prehypophysial arteries near the anterior end of the postoptic nucleus. In the neurohypophysis, abundant presumptive AVT-fibers terminated in the posterior dorsal wall, although some fibers terminated in the anterior dorsal wall and only a few fiber endings were found in the ventral wall. No ACTH-positive cells were detected in the hagfish brain or in the pituitary gland.
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  • 81
    ISSN: 1432-0878
    Keywords: Baboon ; Pituitary gland ; Gonadotrophs ; Mammotrophs ; Somatotrophs ; Thyrotrophs ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The identification and regionalization of four pituitary parenchymal cell types, gonadotrophs, mammotrophs, somatotrophs and thyrotrophs, were studied in the baboon (Papio cynocephalus) hypophysis using immunocytochemistry. The gonadotrophs were homogeneously distributed throughout the entire pars distalis. Both mammotrophs and somatotrophs predominate at the superior and inferior poles of the organ. The medial and anteromedial regions are populated by mammotrophs and thyrotrophs, while the lateral and posterior portions of the pars distalis contain large numbers of somatotrophs.
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  • 82
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    Cell & tissue research 232 (1983), S. 129-140 
    ISSN: 1432-0878
    Keywords: Serotonin ; Optic lobes ; Insecta ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The cellular localization of 5-HT in the optic lobes of three insect species was assayed with the use of antibodies raised against 5-HT. In Schistocerca, Periplaneta, and Calliphora all neuropil regions of the optic lobe, the lamina, medulla and lobula, contain 5-HT-immunoreactive varicose fibres in different patterns, like columns and layers. Such fibres also connect the lobula to neuropil in the lateral protocerebrum. In Calliphora also 5-HT-positive fibres of the medulla and lobula plate have projections to the lateral protocerebrum, whereas the origin of the lamina fibres is not certain. In all species the processes displaying 5-HT-like immunoreactivity appear to be derived from a relatively small number of cell bodies, each neuron thus having processes over a large volume of the neuropil of the optic lobe in different layers.
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  • 83
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    Cell & tissue research 234 (1983), S. 439-450 
    ISSN: 1432-0878
    Keywords: Avidin ; Avidin-biotin interaction ; Biotin affinity histochemistry ; Biotin hydrazide ; Immunocytochemistry ; Magnum gland ; Secretion ; Oviduct
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The location of endogenous avidin was studied cytochemically in the magnum tissue of the oviduct of laying hens. Two methods, based on an interaction of avidin-biotin with biotin hydrazide-peroxidase (B-HRP) as an affinity reagent, and on an immunoperoxidase technique, were tested by morphological analysis. The data obtained by both methods showed that in the magnum B-HRP is a strictly substitutive reagent for endogenous avidin. Avidin was clearly demonstrated in large amounts in the secretory granules of some epithelial cells and tubular gland cells, but was absent from mucous cells, the goblet cells, which had been believed to be the location of avidin production, and from ciliated cells. These granules had previously been demonstrated by both electron-microscopic cytochemical techniques. Especially in acinar cells, they were nonhomogeneous with a speckled core and a dense peripheral part. They ranged in size from 500 to 2200nm in diameter in the gland and 180 to 720 nm in the epithelium. Columnar epithelial cells containing avidin granules had a strong resemblance to those of the protodifferentiated tubular gland cells in the magnum of chicks pretreated with daily estrogen or estrogen plus progesterone, and might have migrated towards the acinus as substitutional secretory cells. Therefore, the acinar cells of the magnum, considered to be composed of several secretory protein-producing systems, are dependent on estrogen and/or progesterone in the oviduct of the laying hen.
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  • 84
    ISSN: 1432-0878
    Keywords: Pituitary ; Adenohypophysis ; Luteinizing hormone (LH) ; Immunocytochemistry ; Lamprey
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A mammalian-like immunoreactive luteinizing hormone (LH) has been localized in the cytoplasm of cells in the meso-adenohypophysis of the sea lamprey, Petromyzon marinus L. Specific staining for LH was absent in other regions of the lamprey pituitary.
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  • 85
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    Cell & tissue research 231 (1983), S. 527-532 
    ISSN: 1432-0878
    Keywords: S-100 ; Müller cells ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The localization of S-100 protein in the retina was studied by immuno-electron microscopy. The immunoreaction for S-100 is specifically confined to Müller cells and astrocytes. Long slender cytoplasmic processes of Müller cells and their participation in the formation of the inner and outer limiting membrane were clearly demonstrated with this immunoreaction. The cytoplasmic processes were also revealed to form continuous sheets surrounding blood vessels. Some somata of Müller cells were found in the outer nuclear layer. The results show that immunostaining for S-100 is useful for selective visualization of Müller cells in the mature retina.
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  • 86
    ISSN: 1432-0878
    Keywords: Angiotensinogen ; Liver ; Kidney ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The renin substrate, angiotensinogen, was localized by immunocytochemistry in liver and kidney of normal rats by the use of an antiserum directed against pure rat angiotensinogen. This substrate was also examined in rats after bilateral nephrectomy, which is known to increase plasma angiotensinogen, and in rats treated with colchicine, which inhibits serum protein secretion. In normal rat liver, light microscopy showed the presence of immunoreactive material in a very few cells. The number of stained hepatocytes rose in rats treated with colchicine or after bilateral nephrectomy. Immuno-staining increased further when rats were both nephrectomized and colchicine treated. In the kidney, angiotensinogen was specifically located as granular formations in nephrocytes of the proximal tubule but never in the granular cells of the juxtaglomerular apparatus. The localization of these granular formations under the brush border suggests that angiotensinogen is reabsorbed from the glomerular ultrafiltrate rather than synthesized in the kidney.
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  • 87
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    Cell & tissue research 233 (1983), S. 471-474 
    ISSN: 1432-0878
    Keywords: Prolactinergic neurons ; Immunocytochemistry ; Cerebral ganglion ; Styela plicata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Peptidergic neurons have been detected in the cerebral ganglion of the ascidian Styela plicata by means of cytochemical methods. After incubation with a mammalian antibody to human prolactin the perikarya show a strong immunoreactivity. The possible function of prolactin-like peptides in the nervous system of protochordates is discussed.
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  • 88
    ISSN: 1432-0878
    Keywords: Fibronectin ; Lung ; Development ; Ultrastructure ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In a previous study changes in the macrodistribution of fibronectin during rat-lung development were examined. Using the peroxidase-antiperoxidase immunocytochemical technique, we have demonstrated the presence of fibronectin in embryonic, neonatal, and adult rat lung at the ultrastructural level. In the embryo, fibronectin is found both in an intra-and extracellular association with isolated pneumoblasts, and in a periodic distribution along the basal lamina. The neonate displays fibronectin in an intracellular association with early type-I cells and on their basal and luminal surfaces, but not in association with type-II cells. Neonatal basal lamina is diffusely labeled by anti-fibronectin antiserum. Fibronectin in adult tissue is found both intracellularly and on the basal and luminal surfaces of type-I cells but not in type-II cells. The basal lamina and interstitial connective tissue are slightly or non-reactive. These observations confirm and extend our initial suggestion that fibronectin is involved in rat-lung development.
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  • 89
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    Cell & tissue research 234 (1983), S. 547-559 
    ISSN: 1432-0878
    Keywords: Myelin proteolipids ; Oligodendrocytes ; Golgi apparatus ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Purified antibodies directed against myelin proteolipids were isolated by affinity chromatography of whole serum obtained from rabbits inoculated with myelin. These antibodies were specific for light, medium and dark oligodendrocytes. Astrocytes, neurons and their processes were not reactive. Immunocytochemical investigations showed that the membranes of the Golgi complex are highly labeled by these antibodies. Diffuse cytoplasmic labeling was only observed on the light and medium oligodendrocytes and was absent from the dark types. Vesicles possessing a punctate staining were detected in the vicinity of the Golgi complex and the oligodendroglial membrane. A discontinuous labeling of the plasmalemma appears to be characteristic of the actively myelinating light and medium oligodendrocytes. In compact myelin sheaths positive immunostaining was only detected at the dense line. The immunocytochemical localization of the myelin proteolipids in the oligodendrocytes is in accordance with previously published biochemical data.
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