ALBERT

Description: Since mineral supplements for horses commonly contain macro minerals, although the requirement for such is usually covered by roughage-based diets, the aim of this study was to investigate the effects of different dietary calcium levels on mineral serum concentrations and bone metabolism. The trial was conducted in 30 young warmblood stallions (2–3 years) that were divided into two groups for a five-month feeding trial. The groups were fed a hay- and oat-based diet and were either supplied with high (Ca-High) or moderate (Ca-Moderate) calcium excess. While in Ca-High calcium supply was about 2–2.5-fold of the requirement, in Ca-Moderate calcium requirements were slightly surpassed (1.5–1.6-fold). In order to monitor the effects of the different calcium supply, blood samples were taken during the trial and analysed for levels of macro and trace elements as well as concentrations of two bone markers. In Ca-Moderate a trend towards higher phosphorus serum levels compared to Ca-High was observed which was significant at the end of the trial (p = 0.0002). Furthermore, results showed no influence of the diet on bone markers. Results support the idea that forage-based rations for horses do not necessarily have to be supplemented with macro minerals but with trace elements.

Description: Transport infrastructure (TI) plays a crucial role in socioeconomic development. The increase of TI inequality, an all-pervading phenomenon in both developed and developing countries, has been an obstacle to sustainable economic growth. The relationship between TI inequality and economic growth has attracted considerable interest over the past three decades. However, the relationship remains obscure, and people find it impossible to utilize to develop economies. This study collected a panel of empirical data from 1982 to 2015 from China to calculate the Gini coefficient and conduct the Granger causality test. The data analysis results show that TI inequality is not always conducive to economic growth. A softening TI inequality helps address the issues of uneven economic growth across regions in the long term. The short-term effects of improving TI inequality at the national level are reflected in the network effect. In addition, the “social filters” facilitate the region to absorb the economic benefits brought by the improvement of TI inequality. These findings offer a way to address the increase of TI inequality and shed light on the ways to improve transport investment from the perspective of economic growth.

Description: This case series study aimed to evaluate the safety, feasibility, and positive outcome of the neurorehabilitation multimodal protocol (NRMP) in 16 chronic post-surgical IVDD Hansen type I dogs, with OFS 0/DPP− (n = 9) and OFS 1/DPP+ (n = 7). All were enrolled in the NRMP for a maximum of 90 days and were clinically discharged after achieving ambulation. The NRMP was based on locomotor training, functional electrical stimulation, transcutaneous electrical spinal cord stimulation, and 4-aminopyridine (4-AP) pharmacological management. In the Deep Pain Perception (DPP)+ dogs, 100% recovered ambulation within a mean period of 47 days, reaching OFS ≥11, which suggests that a longer period of time is needed for recovery. At follow-up, all dogs presented a positive evolution with voluntary micturition. Of the DPP− dogs admitted, all achieved a flexion/extension locomotor pattern within 30 days, and after starting the 4-AP, two dogs were discharged at outcome day 45, with 78% obtaining Spinal Reflex Locomotion (SRL) and automatic micturition within a mean period of 62 days. At follow-up, all dogs maintained their neurological status. After the NRMP, ambulatory status was achieved in 88% (14/16) of dogs, without concurrent events. Thus, an NRMP may be an important therapeutic option to reduce the need for euthanasia in the clinical setting.

Description: The threshing of frozen corn is accompanied by breakage and adherence, which influence the cleaning performance when the corn-cleaning mixture is separated and cleaned. In order to reduce the impurity ratio and loss ratio during frozen corn cleaning and provide theoretical support for frozen corn combine harvesting, this study employed a self-made air-screen cleaning system with adjustable parameters. The optimal process parameters of frozen corn cleaning were determined by using the response surface method (RSM). The influences of the fan speed (FS), vibrational frequency (VF), and screen opening (SO) on the cleaning performance were explored. The results showed that all three process parameters had significant effects on the impurity ratio (IR) and loss ratio (LR). The fan speed had the most significant impact. The cleaning performance was optimal when the fan speed was 102.7 rad/s, the vibration frequency was 6.42 Hz, and the screen opening was 21.9 mm, corresponding to a 0.80% impurity ratio and a 0.61% loss ratio. The predicted values of the regression models were consistent with the experimental results with a relative error of less than 5%. The reliability and accuracy of regression models were established and confirmed.

Description: The muskrat is a neozoon species that has occupied many countries of continental North Europe after its introduction from north America as fur animals. Due to its burrowing activity it damages river and canal banks and structures of flood control. For this reason, the eradication of this alien species is recommended. Muskrats are also of parasitological interest since they can act as suitable intermediate hosts for Echinococcus multilocularis. On the other hand, little is known on the other helminths that infect muskrats. A total of 130 muskrats of different age groups trapped in different habitats in the Barnim district of the Brandenburg state by a professional hunter were examined for parasites and seven trematodes (Echinostoma sp., Notocotylus noyeri, Plagiorchis elegans, Plagiorchis arvicolae, Psilosostoma simillimum, P. spiculigerum, Opisthorchis felineus and four larval cestode species (Hydatigera taeniaeformis, Taenia martis, Taenia polyacantha, Taenia crassiceps) were detected. Larval stages of E. multilocularis were not found. O. felineus was found for the first time in muskrats in Germany. All the named parasites were present in Europe prior to the introduction of muskrats. With a prevalence of 48.9%, Strobilocercus fasciolaris, the larval stage of the cat tapeworm, H. taeniaeformis, was the most frequent parasite found in adult muskrats.

Description: Water uptake is a seminal process in seed germination. Salt and polyethylene glycol (PEG) are known to retard seed germination rates and percentages, which is often attributed to osmotic effects. Here, we quantified water uptake in wheat seeds killed with a hot needle, finding evidence of three distinct water uptake pools. The fast pool was unaffected by salt, and likely represents cell walls and other apoplastic material. Water uptake into the medium and slow pools was slowed by salt addition, with the medium pool thought to be cellular, while the slow pool is presumably related to endosperm hydration. Salt caused a minor decrease in the water uptake rates and maximum seed water content, while PEG strongly suppressed both parameters. Seeds transferred between water and salt solutions followed the water uptake trajectories of the solution into which they were transferred. Seeds transferred from PEG to water achieved final seed water contents similar to water control seeds, while seeds transferred from water to PEG achieved significantly higher final water contents than PEG controls. This work confirms that salt and PEG have distinct effects on water uptake by wheat seeds.

Description: The south of Portugal is one of the regions that will be most affected by the impacts of climate change (CC), with an expected increase in water scarcity. Irrigated super-intensive olive orchards occupy a large area of the used agricultural surface in the Alentejo region, south of Portugal, making it necessary to adapt this crop to the effects of CC. This study assessed the impacts of CC and defined adaptation measures concerning irrigation management of the super-intensive olive orchard. To compute the crop irrigation requirement (CIR), the soil water balance model ISAREG was combined with climate data relative to the reference period 1971–2000 and to the representative concentration pathways RCP4.5 and RCP8.5 for the periods 2011–2040, 2041–2070, and 2071–2100. The growing degree-days (GDD) approach was used to estimate olive phenology for these CC scenarios. Unchanged irrigation management with an average CIR increase up to 16% in RCP4.5 and 31% in RCP8.5 is expected. By adopting higher levels of water deficit, water savings of up to 22% can be realized. The results showed that the anticipated CIR increase for the CC scenarios can be mitigated through regulated deficit irrigation strategies.

Description: 5-aminolevulinic acid (ALA), a key precursor in the biosynthesis of porphyrins, can improve plant tolerance to various environmental stresses. However, it is unclear whether ALA can improve tolerance in poplar. Here, we investigated the effects of ALA on poplars under drought and salt stresses. ALA pretreatment exhibited less morphological damage, reduced leaf malonaldehyde content (MDA) and electrolyte leakage (EL), and increased leaf relative water content (RWC), proline (PRO), superoxide dismutase (SOD), and peroxidase (POD) content under stresses. Furthermore, exogenous ALA mitigated the decrease in photosynthetic capacity, and restored the chlorophyll content (Chl), net CO2 assimilation rate, stomatal conductance (Gs), transpiration rate (Tr), maximal photochemical quantum yield of PSII (Fv/Fm), actual quantum yield of photosynthesis (YII), and electron transfer rate (ETR) of poplar under various stresses. qRT-PCR showed that ALA up-regulated the expression of antiporters and aquaporins genes, which are associated with Na+ exclusion in the leaf cells and the transport activity of aquaporins. In summary, ALA pretreatment significantly improved the stress tolerance of poplar, decreasing the degree of membrane lipid peroxidation and promoting the photosynthesis and antioxidant capacity of leaves. In addition, our results showed that ALA might mediate Na+ transporter and aquaporins activity, thereby increasing the salt tolerance of poplar.

Description: A field experiment was conducted to investigate the effectiveness of different herbicides for controlling wild onion (Asphodelus tenuifolius) in cumin (Cuminum cyminum L.) during the rabi seasons (2018–2019 and 2019–2020) at Agricultural Research Station, Agriculture University, Jodhpur, Rajasthan. The experiment comprised eight herbicidal weed management treatments for wild onion applied to cumin in a three-replication randomized block design. Among the herbicidal weed management treatments, early post-emergence (8 DAS) application of oxyfluorfen 200 g/ha resulted in the lowest weed density and dry matter of Asphodilus tenuifolius, with maximum weed (Asphodilus tenuifolius) control efficiency at 40 days after sowing (DAS) during both experimental years. Likewise, the highest total efficiency of weed control was recorded with the application of oxyfluorfen 200 g/ha at 8 DAS. Oxyflourfen 200 g/ha used early post emergence (8 DAS) reduced the weed index more effectively than the other herbicides. It also recorded the highest number of branches/plant, plant height, umbels/plant, umbellates/umbel, seeds/umbellates, and seed yield. However, application of oxyflourfen @ 200 g/ha 8 DAS–early POE and pendimethalin 38.7 CS 500 g/ha + oxyfluorfen @ 150 g/ha 8 DAS–early POE were statistically similar in terms of plant growth, yield, and yield attributes. The net returns (366.49 USD/ha in 2018–2019 and 175.72 USD/ha in 2019–2020) and B:C ratio (1.70 and 1.33 in 2018–2019 and 2019–2020, respectively) were also superior, with oxyfluorfen 200 g/ha applied early post emergence.

Description: This study evaluated the effects of the duration of ZH supplementation and days on feed (DOF) on performance, carcass characteristics, and saleable meat yield of Nellore young bulls. The fixed effects included the duration (0, 20, 30, or 40 d before slaughter plus a 3 d ZH withdrawal period—8.33 mg of ZH/kg of DM) and DOF (90 and 117 d). Feed efficiency (G:F) linearly increased when the duration of ZH supplementation increased (p 〈 0.01). Nellore bulls fed ZH had greater HCW (p 〈 0.01), dressing percentage (p 〈 0.01) and Longissimus muscle area (LMA) (p 〈 0.01), but less 12th-rib fat (p = 0.04) than the control group. The hot carcass weight (HCW) (p 〈 0.01), and dressing percentage increased linearly (p 〈 0.01) with the increase of duration of ZH supplementation. The HCW, ossification, and 12th-rib fat increased with DOF (p 〈 0.01). The ZH supplemented group had most of the individual cuts of hindquarters and total saleable meat increased compared with the control. Zilpaterol hydrochloride was effective in improving hot carcass weight, hindquarter, and saleable meat yields of Nellore bulls when fed for at least 20 d before slaughter, independently of days on feed.

Description: Healthy, weaned, coccidial-free male rabbits from two breeds (New Zealand white (NZ) and V-line (VL)) were divided into 10 equal groups (5 groups each for NZ and VL) (3 replicates/group, 6 rabbits/replicate, 18 rabbits/group). All rabbits were inoculated with 5 × 104 Eimeria spp. oocysts (E. intestinalis (67%), E. magna (22%), and E. media (11%)) except for the rabbits in the first group (G1), which were inoculated with a sterile solution and served as a negative control. The remaining four groups were treated as follows: G2, no treatment/positive control, G3, treated with neem leaf extract, G4, treated with pomegranate peel extract (PPE), and G5, treated with a combination of neem leaf extract and PPE. For both breeds, our results showed that the use of neem leaf and/or pomegranate peel extract resulted in improved growth performance, with a significant improvement in relative feed conversion ratio (FCR) compared to the positive control groups, which recorded the worst values, as well as a significant (p ≤ 0.05) reduction in mean oocyst count compared to the positive control groups. We also observed downregulation of mRNA levels of IL-1βα, IL6, and TNF-α in the herbal treatment groups compared with the mRNA levels of these genes in the positive control groups. Herbal treatment with neem leaf and/or pomegranate peel extracts had positive effects on the NZ and VL rabbits experimentally infected with mixed Eimeria species, as evidenced by their healthy appearance, good appetite, no mortalities, an anticoccidial index 〉 120, and a significantly higher total return and net profit when compared to the positive control groups of both breeds. In NZ rabbits, the treatment with neem leaf extract alone (G3) or in combination with PPE (G5) recorded the most efficient economic anticoccidial activity.

Description: The short form of the leptin receptor (LRa) plays a key role in the transport of leptin to the central nervous system (CNS). Here, the resistin (RSTN)-mediated expression of LRa in the preoptic area (POA), ventromedial and dorsomedial nuclei (VMH/DMH),arcuate nucleus (ARC) and the anterior pituitary gland (AP)was analyzed considering the photoperiodic (experiment 1) and nutritional status (experiment 2) of ewes. In experiment 1, 30 sheep were fed normally and received one injection of saline or two doses of RSTN one hour prior to euthanasia. RSTN increased LRa expression mainly in the ARC and AP during long days (LD) and only in the AP during short days (SD). In experiment 2, an altered diet for 5 months created lean or fat sheep. Twenty sheep were divided into four groups: the lean and fat groups were given saline, while the lean-R and fat-R groups received RSTN one hour prior to euthanasia. Changes in adiposity influenced the effect of RSTN on LRa mRNA transcript levels in the POA, ARC and AP and without detection of LRa in the VMH/DMH. Overall, both photoperiodic and nutritional signals influence the effects of RSTN on leptin transport to the CNS and are involved in the adaptive/pathological phenomenon of leptin resistance in sheep.

Description: One important type of animal welfare concern is “natural living” (i.e., that animals are able to express natural behaviours that are important to them, and to engage with aspects of the natural world that they find important). The aims of this narrative review were to describe the behavioural development of calves (Bos taurus) in natural settings and use this to identify characteristics of natural systems that may be important to consider relative to this natural living conception of animal welfare. At birth, calves are licked by their mothers and soon stand to suckle for colostrum, and during the milk-feeding period, calves spend much of their time lying down. In natural systems, calves perform a variety of social behaviours with herd-mates, and slowly transition from their mother’s milk to eating solid food, by gradually increasing time spent grazing and ruminating. In contrast, on most commercial dairy systems, dairy calves are removed from their mothers at birth, housed individually, fed restricted amounts of milk and weaned abruptly at a young age. The results of this review suggest that accommodating key natural behaviours, for example through the use of teat feeding of milk, social housing, and gradual weaning, can help address welfare concerns.

Description: The aim of the present research was to analyze the farmers’ intention towards participation in the management and conservation of wetlands through the lens of the extended theory of planned behavior (TPB). To do this, a cross-sectional survey of Iranian farmers was carried out. To select the samples, a multi-stage random sampling process with a proportional assignment was employed. The research instrument was a researcher-made questionnaire whose validity and reliability were verified using various quantitative and qualitative indicators. The results of the extended TPB using structural equation modeling showed that four variables, namely moral norms of participation in management and conservation (MNPMC), attitude towards participation in management and conservation (APMC), subjective norms towards participation in management and conservation (SNPMC), and self-concept about participation in management and conservation (SCPMC) had positive and significant impacts on intention towards participation in management and conservation (IPMC). The results also revealed that that entering MNPMC and SCPMC into TPB could increase its explanatory power. Also, the fit indicators supported the extended TPB. From a practical point of view, the present study provides justifications and insights for the use of MNPMC, APMC, SNPMC, and SCPMC in policies and programs intended to encourage farmers and local communities to participate in wetlands management and conservation.

Description: A previous study revealed low survival rates for Japanese larch (Larix kaempferi) summer-planted seedlings grown in Hiko-V-120 containers. This study examines nursery practices that could potentially prevent deterioration of the seedling water balance after planting to improve the survival rate of this species, which has a low drought tolerance. During summer planting, we tested (1) drought hardening or high-potassium fertilization for two months before planting, (2) antitranspirant or topping treatment at planting, and (3) the use of the JFA-150 container with a larger capacity and lower growing density than the Hiko-V-120 container. Drought hardening increased seedling drought tolerance because of the low leaf:root ratio, due to lower leaf mass production, resulting in increased survival from 74% to 93% in Hiko-V-120 containers. When JFA-150 containers were used, the leaf:root ratio was lower because of higher root mass, resulting in an increase in survival to 87%, with the highest survival of 97% when combined with drought hardening. The application of antitranspirant increased survival to over 90%, whereas topping did not, probably because of severer competition from weeds. High-potassium fertilization did not affect seedling traits or survival. For better survival of summer-planted container-grown Japanese larch seedlings, it is recommended that they be grown in containers providing sufficient cell volume and density for root growth while the seedlings are in the nursery and that irrigation be withheld for two months before planting. In addition, to obtain higher survival, an antitranspirant can be applied at planting at a cost.

Description: Contagious agalactia is associated with mastitis, keratoconjunctivitis, arthritis, pneumonia, and septicemia in small ruminants in countries with large dairy industries worldwide. The causative agents belong to four (sub)species of the Mycoplasma genus that have remained essentially susceptible to antimicrobials, including to the widely-used tetracycline family. However, some clinical isolates have been detected that show increased minimum inhibitory concentrations of tetracyclines, although they do not harbor the mutation in the 16SrRNA gene usually associated with resistance. The present work aimed to assess whether efflux pumps, infrequently described in mycoplasmas, could participate in the observed moderate loss of susceptibility. General efflux mechanisms were measured (i) using the fluorescence property of ethidium bromide when accumulated intracellularly and intercalated in the mycoplasma genomes, its active extrusion resulting in a temperature-dependent decrease in fluorescence and (ii) monitoring the growth inhibition of mycoplasmas by subinhibitory concentrations of tetracycline with or without reserpine, a known inhibitor of efflux in other bacteria. Both methods revealed non-specific efflux phenomena in most of the isolates tested, although their efficacy was difficult to quantify. This property could contribute to the acquisition of mutations conferring resistance by maintaining intracellular concentrations of tetracyclines at subinhibitory levels.

Description: Reliable estimations of parameter values and associated uncertainties are crucial for crop model applications in agro-environmental research. However, estimating many parameters simultaneously for different types of response variables is difficult. This becomes more complicated for grapevines with different phenotypes between varieties and training systems. Our study aims to evaluate how a standard least square approach can be used to calibrate a complex grapevine model for simulating both the phenology (flowering and harvest date) and yield of four different variety–training systems in the Douro Demarcated Region, northern Portugal. An objective function is defined to search for the best-fit parameters that result in the minimum value of the unweighted sum of the normalized Root Mean Squared Error (nRMSE) of the studied variables. Parameter uncertainties are estimated as how a given parameter value can determine the total prediction variability caused by variations in the other parameter combinations. The results indicate that the best-estimated parameters show a satisfactory predictive performance, with a mean bias of −2 to 4 days for phenology and −232 to 159 kg/ha for yield. The corresponding variance in the observed data was generally well reproduced, except for one occasion. These parameters are a good trade-off to achieve results close to the best possible fit of each response variable. No parameter combinations can achieve minimum errors simultaneously for phenology and yield, where the best fit to one variable can lead to a poor fit to another. The proposed parameter uncertainty analysis is particularly useful to select the best-fit parameter values when several choices with equal performance occur. A global sensitivity analysis is applied where the fruit-setting parameters are identified as key determinants for yield simulations. Overall, the approach (including uncertainty analysis) is relatively simple and straightforward without specific pre-conditions (e.g., model continuity), which can be easily applied for other models and crops. However, a challenge has been identified, which is associated with the appropriate assumption of the model errors, where a combination of various calibration approaches might be essential to have a more robust parameter estimation.

Description: Urban greenery is widely acknowledged as a key element for creating livable urban environments and improving residents’ quality of life. However, only a few current studies on the subject of urban greenery focus on a human visual perspective and take street greenery into consideration. Street greenery is an indispensable component of urban vegetation to which residents have a higher frequency of access. Additionally, few studies focused on the disparity of the green view at a micro-level, such as at a county or community level. This study explored the spatial distribution of street greenery and its influential factors using the green view index (GVI) as the main evaluation indicator. Compared to other traditional indicators of greenery, such as the normalized difference vegetation index (NDVI) and accessibility, GVI is recognized as a human-oriented indicator to evaluate the quantity of greenery viewed by human eyes in daily life. The downtown area of Shanghai was chosen as the case study, as it reflects the common phenomenon of street greenery in many megacities globally. In addition, county/jiedao (the same administrative area as county in China) level was selected as the minimum geographical unit to evaluate the disparity of GVI and its influential factors to fill the knowledge gap. We analyzed 233,000 pieces of street-view images from Baidu Map and other correlated data. The results showed (1) the street greenery of 70% of the downtown area of Shanghai is less than the recommended comforFogre visual environment; (2) street greenery is spatially clustered in Huangpu district, Xuhui district, college town, and the Century Park of Shanghai; (3) street-greenery distribution is positively correlated with housing price and street network density, and negatively correlated with the ratio of society vulnerability; however, it is uncorrelated to population density. According to these findings, local municipalities could improve urban planning and design by introducing a more human-oriented green-space policy that improves social equity.

Description: Climate change has inherent multidisciplinary characteristics, and predicting the future of a single field of work has a limit. Therefore, this study proposes a water-centric nexus approach for the agriculture and forest sectors for improving the response to climate change in the Korean Peninsula. Two spatial models, i.e., Environmental Policy Integrated Climate and Integrated Valuation of Ecosystem Services and Tradeoffs, were used to assess the extent of changes in agricultural water demand, forest water supply, and their balance at the watershed level in the current and future climatic conditions. Climate changed has increased the agricultural water demand and forest water supply significantly in all future scenarios and periods. Comparing the results with RCP8.5 2070s and the baseline, the agricultural water demand and forest water supply increased by 35% and 28%, respectively. Water balance assessment at the main watershed level in the Korean Peninsula revealed that although most scenarios of the future water supply increases offset the demand growth, a risk to water balance exists in case of a low forest ratio or smaller watershed. For instance, the western plains, which are the granary regions of South and North Korea, indicate a higher risk than other areas. These results show that the land-use balance can be an essential factor in a water-centric adaptation to climate change. Ultimately, the water-centric nexus approach can make synergies by overcoming increasing water demands attributable to climate change.

Description: Sex differences in a variety of cognitive traits have long been reported in various species, including dogs. However, only a few canine studies have taken the possible effect of reproductive hormones into account. The aim of this study was to investigate the effects of sex and reproductive status of pet dogs (N = 1032) on two cognitive traits: inhibitory control and social cognition. Inhibitory control was assessed using the cylinder test, and the dogs’ tendency to initiate social contact with a human during a problem-solving situation was assessed using the unsolvable task. Female dogs had a significantly higher success rate in the cylinder test compared to males, and they spent significantly more time in human-directed behavior during the unsolvable task. In contrast, males spent significantly more time in independent behavior during the unsolvable task. Reproductive status had no significant effect on the results of the cylinder test or the unsolvable task. Our results showed that female dogs asked for more help/used a more cooperative strategy during a problem-solving situation and had greater inhibitory control compared to males. According to our results, it seems likely that these sex differences were not influenced to a large extent by reproductive hormones.

Description: Heritabilities of workability (WT) traits—milking speed (MS) and temperament (MT)—as well as genetic and phenotypic correlations between these traits in the population of Polish Holstein-Friesian (PHF) cows were estimated. The estimation of genetic parameters was performed twice: first with the use of pedigree data; and second with the use of pedigree and genomic data. Phenotypic data from routinely conducted MS and MT evaluations for 1,045,511 cows born from 2004 to 2013 were available; the cows were evaluated from 2011 to 2015. The main dataset was reduced based on imposed restrictions (e.g., on age of calving, stage of lactation and day of first trial milking). The dataset prepared in this manner comprised 391,615 cows. It was then reduced to daughters of 10% randomly selected sires for computational reasons. Finally, for genetic parameter estimation, 13,280 records of cows were used. The linear observation model included additive random effects of animal, fixed effects of herd-year-season of calving subclass (HYS) and lactation phase, fixed regressions on cow age at calving and the percent of HF breed genes in the cow genotype. Heritabilities estimated based on pedigree data were 0.12 (±0.0067) for MS and 0.08 (±0.0063) for MT, the genetic correlation between MS and MT was estimated at 0.05 (±0.0002) and the phenotypic correlation coefficient was estimated at 0.14 (±0.0004). The inclusion of genomic information of sire bulls had no clear effect on the size of the estimated WT genetic parameters. The heritabilities of MS and MT were 0.11 (±0.0065) and 0.09 (±0.0012), respectively. The genetic and phenotypic correlation coefficients were 0.07 (±0.0003) and 0.12 (±0.0005), respectively. The sizes of the obtained heritabilities of WT and of the genetic and phenotypic correlation between these traits indicate the possibility of effective population improvement for both WT traits.

Description: This work aimed to (i) determine the reliability of a portable Bluetooth colourimeter for fruit colour measurements; (ii) characterise the changes in quantitative skin colour attributes in a nectarine cultivar in response to time from harvest; and (iii) determine the influence of row orientation and training system on nectarine skin colour. The skin colour attributes measured with the colourimeter, namely L*, a* and b*, were calibrated and validated against a reference spectrophotometer. C* and h° were obtained from a* and b*. Skin colour was measured in situ from 42 days before to 6 days after harvest on ‘Majestic Pearl’ nectarines subjected to different row orientations and training systems. Validation models showed high reliability of colour estimations. The trends of colour attributes over time were characterised by cubic regression models, with h° proving to be the best parameter to describe changes of colour over time, with a clear link to the maturation process. No significant effects of row orientation and training system on skin colour were observed at harvest. Overall, the device proved reliable for fruit colour detection. Results of this study highlight the potential of h° as a quantitative index to monitor ripening prior to harvest in ‘Majestic Pearl’ nectarines.

Description: Cucumber, a widely cultivated vegetable, is mostly grown under greenhouse conditions. In recent years, the overuse of inorganic fertilizers for higher yield attainment adversely has affected human health and the environment. Therefore, a greenhouse experiment was designed to evaluate the effects of different nutrient sources (poultry manure (PM) and mineral fertilizer (MF)) on productivity-enhancing parameters of cucumber via univariate and multivariate analyses. Amounts of PM and MF (NPK15:15:15) were added to coco-peat per cubic meter by weight/volume (w/v) ratios as follows: T1 (control), 60 kg PM; T2, 30 kg PM + 3 kg MF; T3, 30 kg PM + 5 kg MF, and T4, 30 kg PM + 7 kg MF. The univariate analysis performed on the collected data illustrated the significant enhancement in growth and productivity for the integrated use of PM and MF. Multivariate analyses (correlation, clustering, and Principal Component Analysis) validated the results of univariate analysis by differentiating treatments into two groups. The three treatments obtained a distinguished group from T1 (Control) and did not show significant differences among each other, with a maximum yield increase by T2 (74.6%). According to these results, T2 could improve cucumber productivity under greenhouse conditions. It can be taken as recommendations for better quality and yield enhancement in future improvement programs and cucumber-related farming communities.

Description: Italian dry-cured ham production requires pigs to be slaughtered at 160 ± 16 kg at 9 months of age (control, C). The study explored three alternatives, based on different feeding conditions: (1) allowing pigs to express their growth potential by letting them reach 160 ± 16 kg slaughter weight (SW) at younger slaughter age (SA) (younger Age, YA); (2) allowing pigs to express their growth potential by maximizing their SW at 9 months SA (greater weight, GW); (3) increasing the SA required to reach 160 ± 16 kg SW (older age, OA). Pigs (336 C21 Goland, 95 kg initial body weight) were slaughtered on average at 257, 230, 257, and 273 d SA and 172.7, 172.3, 192.9, and 169.3 SW kg for the four treatments, respectively. C pigs had an average daily gain (ADG) of 715 g/d and feed efficiency (FE) of 0.265 (gain to feed). Compared to C, YA pigs had higher ADG (+32%), FE (+7.5%), and better ham adiposity; GW pigs had higher carcass weight (+12%), ADG (+25%), trimmed ham weight (+10.9%), and better ham adiposity. OA treatment affected ADG (−16.4%), FE (−16.6%), and trimmed ham weight (−3.6%). YA and GW could be promising alternatives to C as they improved FE and ham quality traits.

Description: Many factors govern the flow of deposited nitrogen (N) through forest ecosystems and into stream water. At the Fernow Experimental Forest in WV, stream water nitrate (NO3−) export from a long-term reference watershed (WS 4) increased in approximately 1980 and has remained elevated despite more recent reductions in chronic N deposition. Long-term changes in species composition may have altered forest N demand and the retention of deposited N. In particular, the abundance and importance value of Acer saccharum have increased since the 1950s, and this species is thought to have a low affinity for NO3−. We measured the relative uptake of NO3− and ammonium (NH4+) by six important temperate broadleaf tree species and estimated stand uptake of total N, NO3−, and NH4+. We then used records of stream water NO3− and stand composition to evaluate the potential impact of changes in species composition on NO3− export. Surprisingly, the tree species we examined all used both mineral N forms approximately equally. Overall, the total N taken up by the stand into aboveground tissues increased from 1959 through 2001 (30.9 to 35.2 kg N ha−1 yr−1). However, changes in species composition may have altered the net supply of NO3− in the soil since A. saccharum is associated with high nitrification rates. Increases in A. saccharum importance value could result in an increase of 3.9 kg NO3−-N ha−1 yr−1 produced via nitrification. Thus, shifting forest species composition resulted in partially offsetting changes in NO3− supply and demand, with a small net increase of 1.2 kg N ha−1 yr−1 in NO3− available for leaching. Given the persistence of high stream water NO3− export and relatively abrupt (~9 year) change in stream water NO3− concentration circa 1980, patterns of NO3− export appear to be driven by long-term deposition with a lag in the recovery of stream water NO3− after more recent declines in atmospheric N input.

Description: Biochar, a pyrogenic carbon, has been receiving incremental attention for potential contribution to soil health, agricultural productivity enhancement while mitigating climate change by sequestering carbon and reducing greenhouse gas (GHG) emissions. However, it is not well-known to us how far rice husk biochar (RHB) application rates could increase phosphorus (P) bioavailability and plant performance when co-applied with P and lime. Here, we present data of a pot experiment consisting of eleven treatments to evaluate RHB, lime, and phosphorus effect on soil phosphorus availability, CO2 emission, nutrient uptake, and yield performance of maize. Co-application of RHB (10 and 15 t ha−1) and lime (100% and 75%) was made with different rates of P (100%, 75%, and 50%). Our result revealed that, at harvest, the combined application of RHB, lime, and phosphorus fertilizer significantly increased soil pH, P availability and decreased Al and Fe toxicity relative to the control while increasing maize yield. The maximum soil pH increased by 36.75%, the highest available P increased by 158.75%, whilst, the exchangeable Al content reduced by 96.84% compared to the control treatment. However, the difference in biomass production and yield among different lime, RHB, and P were minimal, with the largest grain yield (15.50 t ha−1) was recorded in the T6 treatments (75% lime + 10 t ha−1 RHB + 100% Triple superphosphate). The increment in biomass and grain yield could have occurred due to lime and RHB mediated changes in soil properties, including enhancement of soil pH, availability of P, and other nutrients. This increased availability then increased nutrient uptake and biomass production. Our results suggest that the combined application of lime and RHB could bring favorable changes in soil properties while sacrificing some carbon from soils.

Description: The introduction of new species in forest management must be undertaken with a degree of care, to help prevent the spread of invasive species. However, new species with higher profitability are needed to increase forest products value and the resilience of rural populations. Paulownia tomentosa has an extremely fast growth. The objective and novelty of this work was to study the potential use of young Paulownia trees grown in Portugal by using heat treatment to improve its properties, thereby allowing higher value applications of the wood. The average chemical composition of untreated and heat-treated wood was determined. The extractive content was determined by successive Soxhlet extraction with dichloromethane (DCM), ethanol and water as solvents. The composition of lipophilic extracts was performed by injection in GC-MS with mass detection. Insoluble and soluble lignin, holocellulose and α-cellulose were also determined. Physical (density and water absorption and dimensional stability) and mechanical properties (bending strength and bending stiffness) and termite resistance was also determined. Results showed that extractive content increased in all solvents, lignin and α-cellulose also increased and hemicelluloses decreased. Compounds derived from the thermal degradation of lignin were found in heat-treated wood extractions. Dimensional stability improved but there was a decrease in mechanical properties. Resistance against termites was better for untreated wood than for heat-treated wood, possibly due to the thermal degradation of some toxic extractives.

Description: This paper describes the physical and tensile properties of corn bracts during a whole harvest period by using two corn cultivars, aiming to realize efficient peeling with minimum energy performance and decrease the incidence of ear damage. The value range and change rule of tensile properties were obtained by combining mechanical experiments and numerical statistics. Meanwhile, mathematical models were established for tensile properties depending on bract moisture content and bract thickness. The experimental results show that the tensile properties of leaf blade were affected by the orientation between pulling force and longitudinal vein, and that parallel orientation was greater than perpendicular. Further, the tensile properties of leaf sheaths depended on the angle between pulling force and natural growth direction of the bract in the following order: 0° 〉 90° 〉 180°. A larger pulling force angle can improve the probability of bract fracturing at the root of leaf sheaths, which helps reduce bract residue on the peduncle. In addition, the fracture mechanisms of leaf blades and sheaths were expressed from physiological and morphological perspectives. The experimental results are believed to be able to provide theoretical guidance by which to design and optimize corn-peeling devices.

Description: The accurate and timely assessment of pasture quantity and quality (i.e., nutritive characteristics) is vital for effective pasture management. Remotely sensed data can be used to predict pasture quantity and quality. This study investigated the ability of Sentinel-2 multispectral bands, convolved from proximal hyperspectral data, in predicting various pasture quality and quantity parameters. Field data (quantitative and spectral) were gathered for experimental plots representing four pasture types—perennial ryegrass monoculture and three mixtures of swards representing increasing species diversity. Spectral reflectance data at the canopy level were used to generate Sentinel-2 bands and calculate normalised difference indices with each possible band pair. The suitability of these indices for prediction of pasture parameters was assessed. Pasture quantity parameters (biomass and Leaf Area Index) had a stronger influence on overall reflectance than the quality parameters. Indices involving the 1610 nm band were optimal for acid detergent fibre, crude protein, organic matter and water-soluble carbohydrate concentration, while being less affected by biomass or LAI. The study emphasises the importance of accounting for the quantity parameters in the spectral data-based models for pasture quality predictions. These explorative findings inform the development of future pasture quantity and quality models, particularly focusing on diverse swards.

Description: The physical-geographical features of the mountainous area of Montenegro cause difficulties in farmers’ life and work. The organization of the agricultural production faces a number of problems that limit the overall development of rural areas. Some agricultural crops, such as raspberry, have found optimal growth conditions and produce appropriate yields associated with good fruit quality in such mountainous area. The Willamette variety dominates the production and has broadly expanded, as some new varieties, most notably, Tulameen, Fertȍdi Zamatos, and Glen Ample. The aim of this paper was to examine the biological and production characteristics of two raspberry varieties—one floricane (Tulameen) and one primocane (Polka)—grown in two localities. We confirmed the general rule that at lower altitudes, these varieties (570 m a.s.l, Bijelo Polje) are characterized by earlier vegetation, flowering, and fruit ripening in comparison to plants at higher altitude (1040 m a.s.l, Mojkovac). The Tulameen variety started flowering on 15 May in Bijelo Polje (the flowering phenophase lasted for 29 days, until 12 June) and on 25 May in Mojkovac (the flowering phenophase lasted for 27 days, until 20 June). That is a delay of 11 days in relation to different locations. The Polka variety started flowering on 25 June in Bijelo Polje (57 days, until 20 August) and on 1 July in Mojkovac (flowering for 67 days, until 5 September). That is a delay of 7 days. The Tulameen variety started maturation on 14 June in Bijelo Polje (maturation lasted for 27 days, until 10 July) and on 22 June in Mojkovac (26 days, until 17 July), with a delay of 9 days. The Polka variety started maturation on 23 July in Bijelo Polje (the maturation phenophase lasted for 55 days, until 15 September) and on 5 August in Mojkovac (52 days, until 25 September), with a delay of 14 days. The results showed that the Polka variety had significantly higher total phenol content than the Tulameen variety (4.43 and 4.03 mg, respectively). In terms of locality, the Mojkovac raspberries had higher total phenol content than the Bijelo Polje raspberries. Polka raspberries also had a higher content of total flavonoids than Tulameen, whereas differences between localities in regard to the content of total flavonoids in the fruit were not significant. The content of condensed tannins and gallotannins in the raspberry fruit was similar in relation to both varieties and localities. Finally, the total antioxidant capacity was significantly higher in the Polka compared to the Tulameen variety, whereas the differences between localities were not statistically significant.

Description: The black soil of Northeast China has a strong adhesion ability because of its unique physical properties, and the soil often adheres to the surface of the grouser shoes of tracked vehicles during the operation. The adhesion performance depends largely on structure parameters of the grouser shoes. The grouser height, the grouser thickness, and the grouser splayed angle were selected as structure parameters. The adhesion force and adhesive soil mass were selected as indicators of adhesion performance. Therefore, the mathematical model between structure parameters and response indicators was established by the response surface method (RSM). The optimal parameters combination was that the grouser height was 20 mm, grouser thickness was 6.34 mm, and grouser splayed angle was 40.45°. The average data of verification experiments occurred when the adhesion force reached 1.11 kPa and adhesive soil mass reached 22.68 g. Compared with the average value of un-optimized experiment results, the adhesion force and adhesive soil mass reduced by 12.84% and 4.63%, respectively. The relative error of the predicted values and measured values was less than 5%, proving the reliability of the regression models. This study could provide a reference method for parameters optimization, and a new structure of the grouser shoes of tracked vehicles will be designed to reduce adhesion.

Description: Gram negative organisms are frequently isolated from Caretta caretta turtles, which can act as reservoir species for resistant microorganisms in the aquatic environment. C. caretta, which have no history of treatment with antimicrobials, are useful sentinel species for resistant microbes. In this culture-based study, commensal bacteria isolated from oral and cloacal samples of 98 healthy C. caretta were compared to clinical isolates from the wounds of 102 injured animals, in order to investigate the presence of AMR bacteria in free-living loggerheads from the Adriatic Sea. A total of 410 isolates were cultured. Escherichia coli and genera such as Serratia, Moraxella, Kluyvera, Salmonella were isolated only in healthy animals, while Acinetobacter, Enterobacter, Klebsiella and Morganella were isolated only from the wounds of the injured animals. When tested for susceptibility to ampicillin, amoxicillin + clavulanic acid, ceftazidime, cefuroxime, gentamicin, doxycycline, ciprofloxacin and enrofloxacin, the clinical isolates showed highly significant differences in AMR rates vs. commensal isolates for all the drugs tested, except for doxycycline. The detection of high AMR rates in loggerheads is of clinical and microbiological significance since it impacts both the choice of a proper antibiotic therapy and the implementation of conservation programs.

Description: The chemical composition of plant components of three maize hybrids harvested at the beginning of six reproductive stages of maturity was compared. The hybrids evaluated included Maximus VIP3, Defender VIP and Feroz VIP, which were evaluated at each of following stages: R1 (grain formation), R2 (milky grain), R3 (pasty grain), R4 (floury grain), R5 (hard grain) and R6 (ripe grain). The advancement in maturation was linearly related to the crude protein (CP) content of the stem, whole plant, and leaves, and there was a difference among the hybrids. Between R4 and R5 stages, Maximus and Defender presented the highest CP contents for husk (6.58 and 5.42% for Maximus; 5.54 and 5.17% for Defender). The neutral detergent fiber (NDF) of the leaves showed a quadratic relationship with the advancement of maturation but did not differ among the hybrids. For all the hybrids, the NDF content in the husk and cobs increased linearly during the reproductive stages (〉77 and 78%, respectively, for the three hybrids in R6). Defender had the lowest NDF content of the cob in R3. The acid detergent lignin contents did not differ among stages in the stems, and showed a linear decrease throughout the whole plant, though the contents did not differ among the hybrids. Due to the differences observed, recommendations for harvest based on the maturity stage for each hybrid should be taken into consideration. There seems to be no important distinction among hybrids for harvesting and use of straw. Despite the reduction in grain yield, an early harvest for earlage or snaplage can provide lower lignin content in husk and cob, as well as higher protein content in the husk, favoring the nutritional value of the vegetative fraction (husk and/or cob).

Description: Rice production in Tanzania, with 67% of its territory considered semi-dry and having average annual rainfall of 300 mm, must be increased to feed an ever-growing population. Water for irrigation and low soil fertility are among the main challenges. One way to decrease water consumption in paddy fields is to change the irrigation regime for rice production, replacing continuous flooding with alternate wetting and drying. In order to assess the impact of different irrigation regimes and nitrogen fertilizer applications on growth, yield, and water productivity of rice, a greenhouse pot experiment with soil from lowland rice ecology was conducted at Sokoine University of Agriculture, Tanzania during the 2019 cropping season. The experiment was split-plot based on randomized complete block design with 12 treatments and 3 replications. Water regimes were the main factors comparing continuous flooding (CF) and alternate wetting and drying (AWD) with nitrogen fertilizer levels as the subfactor, comparing absolute control (no fertilizer) with 0 (P and K fertilizers), 60, 90, 120, and 150 kg Nha−1. Alternate wetting and drying (AWD) significantly improved water productivity by 8.3% over CF (p 〈 0.05). Water productivity (WP) ranged from 0.6 to 1.5 kg of rice per m3 of water. Average water use ranged from 36 to 82 L per season, and water saving was up to 34.3%. Alternate wetting and drying significantly improved yields (p 〈 0.05) by 13.3%, and the yield ranged from 21.8 to 118.2 g pot−1. The combination of AWD water management and 60 kg N ha−1 nitrogen fertilization application was found to be the optimal management, however there was no significant difference between 60 and 90 kg N ha−1, in which case 60 kg N ha−1 is recommended because it lowers costs and raises net income. Nitrogen levels significantly affected water productivity, water use, and number of irrigations. Nitrogen levels had significant effect (p 〈 0.05) on plant height, number of tillers, flag leaf area, chlorophyll content, total tillers, number of productive tillers, panicle weight, panicle length, 1000-grain weight, straw yield, grain yield, and grain harvest index. The results showed that less water can be used to produce more crops under alternative wetting and drying irrigation practices. The results are important for water-scarce areas, providing useful information to policy makers, farmers, agricultural departments, and water management boards in devising future climate-smart adaptation and mitigation strategies.

Description: The knowledge of genetic variability and breeding techniques is crucial in crop improvement programs. This information is especially important in underutilized crops such as Bambara groundnut, which have limited breeding systems and genetic diversity information. Hence, this study evaluated the genetic variability and established the relationship between the yield and its components in Bambara groundnut based on seed weight using multivariate analysis. A field trial was conducted in a randomized complete block design with three replications on 28 lines. Data were collected on 12 agro-morphological traits, and a statistical analysis was conducted using SAS version 9.4 software, while the variance component, genotypic and phenotypic coefficient variation, heritability, and genetic advance values were estimated. A cluster analysis was performed using NT-SYS software to estimate the genetic relations among the accessions. The results showed significant variability among the accessions based on the yield and yield component characteristics. The evaluated lines were grouped into seven primary clusters based on the assessed traits using the UPGMA dendrogram. Based on the overall results, G5LR1P3, G1LR1P3, G4LR1P1, G2SR1P1 and G3SR1P4 performed the best for the yield and yield components. These improved lines are recommended for large-scale evaluation and utilization in future breeding programs to develop high-yield Bambara groundnut varieties.

Description: Root-knot nematode (RKN) is a serious threat to crops worldwide due to the difficulty in controlling it and the limited eco-friendly alternatives to deal with the biotic stress it causes. In the present work, water-extractable fractions obtained from vermicompost (WSFv), vermicompost enriched with Trichoderma asperellum (WSFta) and T. virens (WSFtv) were tested as biotechnological tools to reduce the impacts of RKN on gas exchange, water use efficiency (WUE) and nutrient concentration in tomato and bell pepper plants. The plants were infected with 5000 eggs and eventual J2 of RKN and then treated with the water-extractable fractions for seven weeks. It was observed that the addition of WSFta, WSFtv and WSFv increased the CO2 assimilation, stomatal conductance and WUE in the tomato plants. In the bell pepper plants, WSFta, WSFtv, WSFv increased the stomatal conductance, while WUE was higher in the treatment with WSFtv. In fact, the parameters associated with the gas exchange were usually higher in the bell pepper than in the tomato plants. Overall, higher contents of N, Mg, B and Mn were detected when the extracts were applied in both bell pepper and tomato plants. The application of the water-extractable fractions, inoculated or not with Trichoderma, attenuates the RKN damage on the gas exchange parameters and successfully enhanced the nutrient concentration in the infected tomato and bell pepper plants, showing that it could be an important and promising tool for reducing the damage caused by this pathogen. We suggest that both the tomato and pepper plants can cope with the dilemma between growth and stress response via stomata regulation that are modulated by the WSF and Trichoderma.

Description: Animals vary on intrinsic characteristics such as temperament and stress responsiveness, and this information can be useful to experimentalists for identifying more homogeneous subsets of animals that show consistency in risk for a particular research outcome. Such information can also be useful for balancing experimental groups, ensuring animals within an experiment have similar characteristics. In this review, we describe the BioBehavioral Assessment Program at the California National Primate Research Center, which, since its inception in 2001, has been providing quantitative information on intrinsic characteristics to scientists for subject selection and balancing, and to colony management staff for management purposes. We describe the program and review studies relating to asthma, autism, behavioral inhibition, etc., where the BBA Program was used to select animals. We also review our work, showing that factors such as rearing, ketamine exposure, and prenatal experience can affect biobehavioral organization in ways that some investigators might want to control for in their studies. Attention to intrinsic characteristics of subject populations is consistent with the growing interest in precision medicine and can lead to a reduction in animal numbers, savings in time and money for investigators, and reduced distress for the animals.

Description: Research was conducted in connection with the pressure exerted by man on the environment through the use of pesticides. The aim of the study was to assess the impact of pesticides on soil and to evaluate the effect of these changes on greenhouse gas emissions into the atmosphere. The research was carried out on soil sown with oilseed rape. The activity of protease and urease, ammonification, nitrification in soil, as well as CO2 (carbon dioxide) and N2O (nitrous oxide) gas emissions from soil were assessed. The analyses were carried out directly after harvest and 2 months after. Pesticides most frequently negatively affected the tested parameters, in particular enzymatic activities. Of the two herbicides used, Roundup had a stronger negative impact on microbial activity. The application of pesticides, especially the fungicide, resulted in an increase in gas emissions to the atmosphere over time. Pesticides disturbed soil environmental balance, probably interfering with qualitative and quantitative relationships of soil microorganism populations and their metabolic processes. This led to the accumulation of microbial activity products in the form of, among others, gases which contribute to the greenhouse effect by escaping from the soil into the atmosphere.

Description: Land suitability assessment is fundamental in space control planning and land development because of its effects on land use and urban layout. Rainstorms and waterlogging have become one of the most common natural disasters in the coastal areas of China. As a result, the concept of an ecological sponge city was incorporated into the construction of cities in the future. Taking Shenzhen–Shantou special cooperation zone (SSCZ), we constructed a storm flooding model based on the SCS flow generation model and GIS to explore the spatial distribution characteristics of the flooding risk in a rainstorm of 100-year lasting 1 h. Combined with population and economic indicators, a radial basis function (RBF) network was utilized to evaluate the environmental risk, the vulnerability of disaster-bearing bodies, and the rain–flood resilience of sponge cities. The self-organizing feature mapping (SOFM) model was used for cluster analysis. Spatial differences were found in the construction suitability of the study area. A suitable construction area (73.59% of the entire area) was located downtown. The construction of the artificial spongy body in the highest vulnerable area (3.25%) needs to be strengthened. The control construction area (3.3%) is located along the banks of the river, with relatively high risk and low resilience of flood control engineering. Ecological construction (19.85%) serves as the sponge body of ecological buffer. The factors of waterlogging, ecology, population, and economy could be integrated comprehensively by applying neural network methods for urban planning and construction.

Description: The silvopastoral system has the potential to alleviate the negative impacts of heat stress on livestock. Through a preliminary study, we assessed the thermal environment experienced by hair coat lambs, as well as the impacts on their bio-thermal and behavioural responses, when either kept in either the silvopastoral system, or exposed to full sun. Twelve hair coat lambs (Dorper × Santa Ines) were randomly assigned to a silvopastoral system or full sun exposure during the summer (from January to February 2017). Parameters, including air temperature, black globe temperature, relative humidity, wind speed, and ground surface temperature, were measured daily for both thermal environments. From 14:00 to 16:00, lambs kept in silvopastoral areas experienced lower levels of air temperature, radiant heat load, and ground surface temperature. Consequently, they had a lower hair coat surface and lower body rectal temperatures. Lambs exposed to a shaded environment spent more time grazing and walking, and less time standing at rest. In conclusion, lambs kept in a silvopastoral system experienced lower levels of radiant heat load and ground surface temperature. In addition, the animals showed a reduced requirement for evaporative cooling and expressed behaviours that indicated a comfortable thermal environment.

Description: Fusarium head blight (FHB) is one of the major threats for wheat production worldwide. It reduces yield, quality, and feeding value of wheat grains. In addition, mycotoxins produced by Fusarium pathogens can have a negative effect on livestock and human health. The aim of this study was to assess changes in technological quality traits and end-use quality of winter wheat varieties after artificial inoculation with Fusarium spp. over three years. Differences in dough development duration and extensibility were measured as the means of relative reductions due to different environments and varieties’ characteristics. Differences in dough softening during kneading were determined as the means of relative increases due to FHB inoculation. In addition, dough had reduced strength, was stickier, and therefore was more difficult to handle, due to a decrease of the average energy value and resistance to extension in FHB-inoculated wheat, compared to naturally infected plants. Dough development time, stability, and resistance usually varied in a similar way, with FHB-resistant varieties showing a good response to FHB inoculation and maintaining good quality. Increasing the level of Fusarium spp. contamination in more FHB-susceptible wheat varieties worsened their technological quality, primarily, the sedimentation value and the gluten index, and hence had a negative effect on the rheological properties.

Description: Molecular genetic techniques (FISH, RT-PCR, and metagenomic analysis) were used to investigate the comparative functional biodiversity in the prokaryotic complex in grassland and forests’ sod-podzol under polycyclic aromatic hydrocarbon influence. The polluted samples showed a decrease in the biomass of the prokaryotic community representatives and a change in the metabolically active dominants–representatives of the Bacteria and Archaea domains compared to the control samples. The suppression of the metabolic activity of prokaryote cells under the influence of PAHs in sod-podzolic soil under meadow vegetation was more pronounced compared to soils under forest vegetation. The representatives of prokaryotes that are sensitive and resistant to the studied PAHs were identified. The representatives of the phylogenetic groups from the bacterial complex resistant to PAH pollution were Proteobacteria (Alphaproteobacteria), Bacteroidetes, Firmicutes, Chloroflexi, and Thaumarhaeota in the archaeal complex. Representatives of the phylum Acidobacteria and Actinobacteria (Streptosporangiales) are noted among those sensitive to PAH contamination. The presence and expression of the functional alkane monooxygenase (alkB) gene were established in all the experimental variants studied. In the plant variants, the number of copies of alkB genes increased by an order of magnitude and the biomass of metabolically active prokaryotic representatives with the functional alkB gene doubled compared to the unpolluted territories. The copy number index of the alkB gene can be used as one of the parameters when characterizing an ecosystem for the presence of PAH pollutants.

Description: Elevated or unnatural levels of arsenic (As) and phosphorus (P) concentrations in soils and waterbodies from anthropogenic sources can present significant hazards for both natural ecosystems and human food production. Effective, environmentally friendly, and inexpensive materials, such as biochar, are needed to reduce mobility and bioavailability of As and P. While biochar features several physicochemical properties that make it an ideal contaminant sorbent, certain modifications such as mineral-impregnation can improve sorption efficiencies for targeted compounds. Here, we conducted sorption experiments to investigate and quantify the potential utility of magnesium (Mg) for improving biochar sorption efficiency of P and As. We synthesized a Mg-modified walnut shells-derived biochar and characterized its ability to remove As and P from aqueous solutions, thereby mitigating losses of valuable P when needed while, at the same time, immobilizing hazardous As in ecosystems. SEM-EDX, FTIR and elemental analysis showed morphological and functional changes of biochar and the formation of new Mg-based composites (MgO, MgOHCl) responsible for improved sorption potential capacity by 10 times for As and 20 times for P. Sorption efficiency was attributed to improved AEC, higher SSA, chemical forms of sorbates and new sorption site formations. Synthetized Mg-composite/walnut shell-derived biochar also removed 〉90% of P from real samples of wastewater, indicating its potential suitability for contaminated waterbody remediation.

Description: Understanding the factors that result in people becoming dog owners is key to developing messaging around responsible acquisition and providing appropriate support for prospective owners to ensure a strong dog–owner bond and optimise dog welfare. This qualitative study investigated factors that influence pet dog acquisition. Semi-structured interviews were conducted with 142 sets of dog owners/caretakers at 23 Dogs Trust community events. Interviews focused on the motivations and influences that impacted how people acquired their dogs. Transcribed interviews and notes were thematically analysed. Two acquisition types were reported, that each accounted for half of our interviewees’ experiences: planned and unplanned. Whilst planned acquisitions involved an intentional search for a dog, unplanned acquisitions occurred following an unexpected and unsought opportunity to acquire one. Unplanned acquisitions frequently involved a participant’s family or friends, people happening upon a dog in need, or dogs received as gifts. Motivations for deciding to take the dog included emotional attachments and a desire to help a vulnerable animal. Many reported making the decision to acquire the dog without hesitation and without conducting any pre-acquisition research. These findings present valuable insights for designers of interventions promoting responsible acquisition and ownership, because there is minimal opportunity to deliver messaging with these unplanned acquisitions. Additionally, these findings may guide future research to develop more complete understandings of the acquisition process. Further studies are required to understand the prevalence of unplanned acquisitions.

Description: It is important to understand price premiums related to certified raw wood to predict the future of forest certifications from the perspective of forestry enterprises. We focused on identifying the trading roundwood market data in the economic center of power in Tokyo. This study aimed to clarify Tama-certified raw wood prices under the local area certification scheme, forest-certified raw wood prices, and the handling volumes at the Tama Roundwood Market Center in Tokyo. Sales details of the Tama Roundwood Market Center were used to identify the handling volumes, Tama-certified raw wood prices, and FSC (Forest Stewardship Council) or SGEC (Japan-specific Sustainable Green Ecosystem Council)-certified raw wood prices. The FSC and SGEC have operated from the center since 2016. Data were collected from the 2006–2018 fiscal years. Our results showed that the volume of raw wood handled increased due to the regeneration-cutting project conducted by the Tokyo Metropolitan Government. On the other hand, there was no price premium for Tama-certified raw wood under the local certification scheme or for FSC- or SGEC-certified raw wood. Price premiums for forest certifications are necessary for ongoing sustainable forest management. There is a need to increase consumer awareness of forest certifications and to differentiate quality certifications further, and these would likely create price premiums.

Description: Environmental pollution is an important issue in metropolitan areas, and roadside trees are directly affected by various sources of pollution to which they exhibit numerous responses. The aim of the present study was to identify morpho-physio-biochemical attributes of maidenhair tree (Ginkgo biloba L.) and American sycamore (Platanus occidentalis L.) growing under two different air quality conditions (roadside with high air pollution, RH and roadside with low air pollution, RL) and to assess the possibility of using their physiological and biochemical parameters as biomonitoring tools in urban areas. The results showed that the photosynthetic rate, photosynthetic nitrogen-use efficiencies, and photochromic contents were generally low in RH in both G. biloba and P. occidentalis. However, water-use efficiency and leaf temperature showed high values in RH trees. Among biochemical parameters, in G. biloba, the lipid peroxide content was higher in RH than in RL trees, but in P. occidentalis, this content was lower in RH than in RL trees. In both species, physiological activities were low in trees planted in areas with high levels of air pollution, whereas their biochemical and morphological variables showed different responses to air pollution. Thus, we concluded that it is possible to determine species-specific physiological variables affected by regional differences of air pollution in urban areas, and these findings may be helpful for monitoring air quality and environmental health using trees.

Description: Suburban rural landscape multifunction has received increasing attention from scholars due to its high demand and impact on main urban areas. However, few studies have been focused on suburban rural landscape multifunction because of data constraints. The present study quantified the four landscape services based on ecological service system, i.e., regulating function (RF), provision function (PF), culture function (CF), and support function (SF), determined the interaction through the Spearman correlation coefficient, and ultimately identified the landscape multifunction hotspots and dominant functions through overlay analysis. The result indicated that suburban rural communities have exhibited the characteristics of regional multifunction, and the landscape multifunction hotspots accounted for 64.2%; it should be particularly noted that, among single-function, dual-function, and multifunction hotspots, both support function, and culture function was dominant, while only one case was found in which the regulating function was dominant. Furthermore, all landscape functions other than SF-CF exhibited certain correlations. The study suggests that planning and management should be performed in future in combination with landscape multifunction to ensure the sustainable development of suburban rural communities.

Description: Bare soil is a critical element in the urban landscape and plays an essential role in urban environments. Yet, the separation of bare soil and other land cover types using remote sensing techniques remains a significant challenge. There are several remote sensing-based spectral indices for barren detection, but their effectiveness varies depending on land cover patterns and climate conditions. Within this research, we introduced a modified bare soil index (MBI) using shortwave infrared (SWIR) and near-infrared (NIR) wavelengths derived from Landsat 8 (OLI – Operational Land Imager). The proposed bare soil index was tested in two different bare soil patterns in Thailand and Vietnam, where there are large areas of bare soil during the agricultural fallow period, obstructing the separation between bare soil and urban areas. Bare soil extracted from the MBI achieved higher overall accuracy of about 98% and a kappa coefficient over 0.96, compared to bare soil index (BSI), normalized different bare soil index (NDBaI), and dry bare soil index (DBSI). The results also revealed that MBI considerably contributes to the accuracy of land cover classification. We suggest using the MBI for bare soil detection in tropical climatic regions.

Description: Background: The use of wheat and flaxseed to produce omega-3 (ω-3) enriched poultry meat and eggs is very popular in the world. However, wheat and flaxseed contain some anti-nutritional factors (ANFs), and enzymes are usually used to alleviate the deleterious influence of ANFs. Method: A 2 × 3 two factors design was used in the experiment. A total of 540 twenty-week-old Nongda-3 laying hens were randomly allocated to six dietary treatments, two diets (corn/flaxseed and wheat/flaxseed), and three enzymes (enzyme-a contains neutral protease 10,000, xylanase 35,000, β-mannanase 1500, β-glucanase 2000, cellulose 500, amylase 100, and pectinase 10,000 (U g−1); enzyme-b contains alkaline protease 40,000 and neutral protease 10,000 (U g−1); enzyme-c contains alkaline protease 40,000, neutral protease 10,000, and cellulase 4000 (U g−1). Results: There was an interaction between dietary treatment and supplemental enzymes for liver weight and liver inflammatory cytokines of broilers. A significant increase was observed in the fat weight of birds fed a corn diet as compared with a wheat diet. A corn diet and wheat diet with the addition of enzyme-a (p 〈 0.001) showed the highest level of liver fat followed by enzyme-c (p 〈 0.01) and enzyme-b. Moreover, a high level of secretory IL-1β, IL-6, and IL-10 and comparatively higher inflammatory changes in the liver tissue were found in birds fed a corn diet as compared with a wheat diet, and enzyme-b showed more beneficial effects as compared with enzyme-a and -c. The gut microbial composition of hens fed a corn diet was significantly different than that of birds fed a wheat diet. Bacteroides were significantly (p 〈 0.05) abundant in the corn-fed birds as compared with wheat-fed birds. However, Firmicutes were less abundant in the wheat-fed birds than the corn-fed birds (16.99 vs. 31.80%, respectively). The microbial community at the genus level differed significantly in the dietary groups and we observed that Bacteroides are the predominant cecal microbiota. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways of co-factors, carbohydrates, vitamins, protein, and energy were expressed at slightly higher levels in the microbiota of the wheat-fed birds, whereas, metabolic pathways for nucleotides, lipids, and glycine were expressed at higher levels in the wheat-fed birds. Furthermore, expression of the growth and cellular processes pathway and endocrine system pathway levels were predicted to be higher for the wheat-fed group as compared with the corn-fed group. Conclusions: In conclusion, our findings suggest that inflammatory changes in laying birds were mediated by a corn diet with flaxseed and enzymes instead of a wheat diet. Additionally, in the wheat-fed group, enzyme-b and -c showed more encouraging results as compared to enzyme-a.

Description: The purpose of this study was to assess the suitability of the Romanian Albanus hybrid of Sorghum bicolor as a potential functional food ingredient. Ultrasound-assisted extraction in different solvents, together with spectrophotometric and chromatographic methods, was used to monitor the variation in total phenolic and flavonoid content and the antioxidant activity of raw sorghum grains before and during short germination periods (24, 36 and 48 h). The High-Performance Liquid Chromatography with Diode-Array Detection (HPLC-DAD) investigation of the extracts revealed that the ungerminated sorghum grains extracted with methanol had the highest diversity of phenolic compounds, while the total phenolic content (TPC) was the highest after 36 h of germination in both extract types: 1853 mg GAE/100 g for the methanolic extract and 1726 mg GAE/100 g for the ethanolic extract. The findings of this study showed that the TPC of sorghum extracts is strongly correlated with their antioxidant activity and, overall, that the studied extracts presented a good radical scavenger activity, which supports the benefits of alimentary uses of Sorghum bicolor grains.

Description: Genomics comprises a set of current and valuable technologies implemented as selection tools in dairy cattle commercial breeding programs. The intensive progeny testing for production and reproductive traits based on genomic breeding values (GEBVs) has been crucial to increasing dairy cattle productivity. The knowledge of key genes and haplotypes, including their regulation mechanisms, as markers for productivity traits, may improve the strategies on the present and future for dairy cattle selection. Genome-wide association studies (GWAS) such as quantitative trait loci (QTL), single nucleotide polymorphisms (SNPs), or single-step genomic best linear unbiased prediction (ssGBLUP) methods have already been included in global dairy programs for the estimation of marker-assisted selection-derived effects. The increase in genetic progress based on genomic predicting accuracy has also contributed to the understanding of genetic effects in dairy cattle offspring. However, the crossing within inbred-lines critically increased homozygosis with accumulated negative effects of inbreeding like a decline in reproductive performance. Thus, inaccurate-biased estimations based on empirical-conventional models of dairy production systems face an increased risk of providing suboptimal results derived from errors in the selection of candidates of high genetic merit-based just on low-heritability phenotypic traits. This extends the generation intervals and increases costs due to the significant reduction of genetic gains. The remarkable progress of genomic prediction increases the accurate selection of superior candidates. The scope of the present review is to summarize and discuss the advances and challenges of genomic tools for dairy cattle selection for optimizing breeding programs and controlling negative inbreeding depression effects on productivity and consequently, achieving economic-effective advances in food production efficiency. Particular attention is given to the potential genomic selection-derived results to facilitate precision management on modern dairy farms, including an overview of novel genome editing methodologies as perspectives toward the future.

Description: There is a growing demand by the modern consumer for meat containing less fat and lower levels of saturated fatty acids, which are considered to increase the risk of coronary heart disease. In southern Italy, the Gentile di Puglia breed is one of the most common on farms, and the light lambs are often consumed. The study evaluates the effect of a diet containing extruded linseed (Linum usitatissimum) on growth performances, carcass traits, and meat quality in Gentile di Puglia light lambs. Thirty-six male lambs are weaned at about 20 days of age, and divided into three groups—each group is either fed a control diet (C), a diet containing 3% extruded linseed (L), or a diet containing 3% extruded linseed and 0.6% oregano (Origanum vulgare) (L + O). The lambs’ growth performances and the slaughtering and dissection data did not differ between groups. Dietary treatments have no significant effect on the quality and chemical composition of Longissimus lumborum (Ll). The mount of linoleic acid in Ll meat is significantly higher in the L group, and this positively affected the total content of n-3 Polyunsaturated Fatty Acid, as well as the n-6/n-3 ratio. The good results obtained concerning the sensory traits meet the requirements of the market’s consumer.

Description: The onset of COVID has impacted the field of veterinary medicine and the lives of cat guardians in numerous ways, yet the subject remains largely unexplored. The purpose of this study was to better understand cat guardians’ fears and concerns pertaining to veterinary care and obtainment of pet care products and food during the initial lock down phase of the COVID pandemic to better address these concerns now and in the future. To this end, an anonymous online survey was distributed to cat guardians during the first two months of the pandemic. The survey included questions pertaining to guardians’ relationship with their cat and their veterinary related concerns and experiences as a result of the pandemic. Quantitative and qualitative data were collected from 956 participants. The results revealed that the increased amount of time guardians spent with their cat had a positive impact on their bond. Participants’ veterinary related concerns, particularly for participants between 18-29 years of age, centered around availability of their veterinarian for both emergency and non-emergency care. Other concerns included fears about the ability to afford emergency veterinary care and obtain cat food and supplies. Awareness of these concerns can help veterinarians better support cat guardians by directly communicating their continued availability and presence in the face of a pandemic as well as other challenging times.

Description: Laser scanning via LiDAR is a powerful technique for collecting data necessary for Digital Terrain Model (DTM) generation, even in densely forested areas. LiDAR observations located at the ground level can be separated from the initial point cloud and used as input for the generation of a Digital Terrain Model (DTM) via interpolation. This paper proposes a quantitative analysis of the accuracy of DTMs (and derived slope maps) obtained from LiDAR data and is focused on conditions common to most forestry activities (rough, steep terrain with forest cover). Three interpolation algorithms were tested: Inverse Distance Weighted (IDW), Natural Neighbour (NN) and Thin-Plate Spline (TPS). Research was mainly focused on the issue of point data density. To analyze its impact on the quality of ground surface modelling, the density of the filtered data set was artificially lowered (from 0.89 to 0.09 points/m2) by randomly removing point observations in 10% increments. This provides a comprehensive method of evaluating the impact of LiDAR ground point density on DTM accuracy. While the reduction of point density leads to a less accurate DTM in all cases (as expected), the exact pattern varies by algorithm. The accuracy of the LiDAR-derived DTMs is relatively good even when LiDAR sampling density is reduced to 0.40–0.50 points/m2 (50–60 % of the initial point density), as long as a suitable interpolation algorithm is used (as IDW proved to be less resilient to density reductions below approximately 0.60 points/m2). In the case of slope estimation, the pattern is relatively similar, except the difference in accuracy between IDW and the other two algorithms is even more pronounced than in the case of DTM accuracy. Based on this research, we conclude that LiDAR is an adequate method for collecting morphological data necessary for modelling the ground surface, even when the sampling density is significantly reduced.

Description: The following document is The Aquaponic Association’s response to a recent publication on E. coli in Aquaponic and Hydroponic systems. [...]

Description: Emerald ash borer (EAB), (Agrilus planipennis Fairmaire), first identified in 2002 in southeast Michigan, has caused catastrophic ash (Fraxinus spp.) mortality in forests within the core of the invasion and has spread to 35 states and five Canadian provinces. Little is known about persistence and densities of EAB populations in post-invasion sites after most ash trees have died. We monitored EAB populations from 2014 to 2016 using double decker (DD) traps set in the midst of white ash (F. americana) trees in 30 post-invasion sites in southeast and south-central Michigan. Two DD traps were deployed at each site. One trap had a dark green upper prism and light purple lower prism, both baited with cis-3-hexenol lures. The other had two dark purple prisms baited with cis-3-hexenol on the upper prism and Manuka oil on the lower prism. In 2014 and 2016, size and condition of ash trees were recorded and area of live white ash phloem was estimated in an 18-m-radius plot centered around each of the DD traps. Area of live white ash phloem per site ranged from approximately 24 to 421 m2 in 2014 and from 24 to 411 m2 in 2016. Canopy condition of live white ash trees generally improved; 65% and 89% of the trees had healthy canopies (

Description: The perceptions and values that local communities have towards protected areas are of great value for the improvement of these territories’ management. Such perceptions and values are often absent in the conservation planning process, particularly in those privately protected areas that are established in areas where the land tenure system is based not only on ownership but also on customary uses. Drawing on qualitative and quantitative data obtained through semi-structured interviews with key stakeholders and members of communities surrounding a privately protected area in southern Ecuador, we identify that the level of collaboration with the managers, the distance to the protected area, the percentage of untitled land, and the dependence on the resources (customary uses) are among the variables affecting these perceptions and values. Positive perceptions towards protected areas and naturalistic values are developed among those who collaborate with the protected area managers, whereas negative perceptions, and a mix of naturalistic and biospheric values are developed among those who have a sense of a lack of attention to social needs although supporting nature conservation at the same time. The evidence presented shows the importance of matching local peoples’ expectations with conservation goals during the establishment of a protected area.

Description: The purpose of this study was to examine the combined effects of milk intake and physical activity on bone mineral density in adolescents. This study was conducted using data from the 2009–2011 Korea National Health and Nutrition Examination Survey (KNHANES), which provided measurements of bone mineral density (BMD) in addition to basic health-related data. This study included 1061 adolescents aged 13 to 18 years (557 males and 504 females) whose data on milk intake and participation time in moderate to vigorous physical activity were available. BMD was measured by dual-energy X-ray absorptiometry (DXA). Milk intake was assessed using the 24-h recall method, and the levels of physical activity were examined using a questionnaire. The physical activity questions of 2009–2011 KNHANES were based on the Korean version of the International Physical Activity Questionnaire (IPAQ) short form. The subjects were classified into four groups according to milk intake and physical activity level: no milk intake + low-level physical activity group (MnoPlow), no milk intake + high-level physical activity group (MnoPhigh), milk intake + low-level physical activity group (MyesPlow), and milk intake + high-level physical activity group (MyesPhigh). The results of partial correlation controlling for age, body mass index (BMI), and energy intake showed that the BMD variables were associated significantly with physical activity in both males and females. Among males, the MnoPlow group had the lowest BMD in all BMD variables, showing a significant difference from the high-level physical activity groups (MnoPhigh, MyesPhigh) by multiple logistic regression analysis. Among females, the MyesPhigh group showed a significantly higher lumbar BMD value than the other groups. The MnoPlow group had approximately 0.3 to 0.5 times lower odds ratio for median or higher BMD values, compared to MyesPhigh group. These results show that milk intake and physical activity have a combined effect on BMD, and suggest that to achieve healthy bone growth, it is important to encourage both moderate to vigorous physical activity and milk intake during adolescence.

Description: Varicocele is an age-related disease with no current medical treatments positively impacting infertility. Toll-like receptor 4 (TLR4) expression is present in normal testis with an involvement in the immunological reactions. The role of peroxisome proliferator-activated receptor-α (PPAR-α), a nuclear receptor, in fertility is still unclear. N-Palmitoylethanolamide (PEA), an emerging nutraceutical compound present in plants and animal foods, is an endogenous PPAR-α agonist with well-demonstrated anti-inflammatory and analgesics characteristics. In this model of mice varicocele, PPAR-α and TLR4 receptors’ roles were investigated through the administration of ultra-micronized PEA (PEA-um). Male wild-type (WT), PPAR-α knockout (KO), and TLR4 KO mice were used. A group underwent sham operation and administration of vehicle or PEA-um (10 mg/kg i.p.) for 21 days. Another group (WT, PPAR-α KO, and TLR4 KO) underwent surgical varicocele and was treated with vehicle or PEA-um (10 mg/kg i.p.) for 21 days. At the end of treatments, all animals were euthanized. Both operated and contralateral testes were processed for histological and morphometric assessment, for PPAR-α, TLR4, occludin, and claudin-11 immunohistochemistry and for PPAR-α, TLR4, transforming growth factor-beta3 (TGF-β3), phospho-extracellular signal-Regulated-Kinase (p-ERK) 1/2, and nucleotide-binding oligomerization domain-like receptor (NLR) family pyrin domain-containing 3 (NLRP3) Western blot analysis. Collectively, our data showed that administration of PEA-um revealed a key role of PPAR-α and TLR4 in varicocele pathophysiology, unmasking new nutraceutical therapeutic targets for future varicocele research and supporting surgical management of male infertility.

Description: Triclosan is a chlorinated phenolic, used in many personal and home care products for its powerful antimicrobial effect. Several studies have shown triclosan toxicity and the American Food and Drug Administration (FDA) in 2016 has limited its use. It has been recently included in endocrine-disrupting chemicals (EDCs), a list of chemicals known for their ability to interfere with hormonal signaling with particular critical effects on reproduction both in animals and humans. In order to deepen the knowledge in this specific field, the present study was undertaken to explore the effect of different concentrations of triclosan (1, 10, and 50 µM) on cultured luteal cells, isolated from swine ovaries, evaluating effects on growth Bromodeoxyuridine (BrDU) incorporation and Adenosine TriPhosphate (ATP) production, steroidogenesis (progesterone secretion) and redox status (superoxide and nitric oxide production, enzymatic and non-enzymatic scavenging activity). A biphasic effect was exerted by triclosan on P4 production. In fact, the highest concentration inhibited, while the others stimulated P4 production (p 〈 0.05). Triclosan significantly inhibited cell proliferation, metabolic activity, and enzymatic scavenger activity (p 〈 0.05). On the contrary, nitric oxide production was significantly increased by triclosan (p 〈 0.01), while superoxide anion generation and non-enzymatic scavenging activity were unaffected.

Description: The main reason for the development of the yield gap in crop production is the inefficient management of nitrogen (N). The nitrogen gap (NG) cannot be ameliorated without an indication and quantification of soil characteristics that limit N uptake by a crop plant. The insufficient supply of N to a plant during its cardinal stages of yield formation is a result of two major-variabilities. The first is spatial variability in the soil characteristics responsible for water supply to a plant, also serving as a nutrient carrier. The second is a vertical variability in soil factors, decisive for pools of available nutrients, and their in-season accessibility to the grown crop. The long-term strategy for NG cover should focus first on soil characteristics (humus stock, pH, nutrient content) responsible for water storage and its availability to the currently grown plant. Diagnostics of plant nutrient availability should deliver data on their contents both in the topsoil and subsoil. The combined use of both classical diagnostic tools and spectral imagery is a way to divide a single field into units, differing in productivity. Remote-sensing techniques offer a broad number of tools to define the in-season crop canopy requirement for fertilizer N in homogenous field units.

Description: Turtles are suspected to be involved in the epidemiology of Leptospira; however, data about the dissemination of this zoonotic pathogen among chelonians are scant. In the present study, the serum samples collected from 49 Trachemys scripta elegans living in a natural park of northern Italy were tested by a microagglutination test to measure detectable antibodies against different Leptospira serovars. Three (6.12%) turtles had agglutinins to the serovar Tarassovi, suggesting that they were exposed to the spirochaetes. Currently, it is not clear if Leptospira can cause disease in chelonians or if these animals can serve as reservoirs of leptospirae. Considering that chelonians often share the same environment with other animals and humans, and considering the One Health perspective, investigations to better understand the role of chelonians as a source of Leptospira infection are necessary.

Description: Control of seed germination and dormancy is important in seed plant adaptation and evolution. When studying seed dormancy of Quercus species, we observed a substantially delayed shoot emergence following a fast root emergence in Quercus chungii F.P.Metcalf. Since epicotyl physiological dormancy (PD) has not been reported in Quercus section Cyclobalanopsis, we examined seed morphology and germination in Q. chungii and aimed to document epicotyl PD in the seeds. The embryo was fully developed in fresh ripe seeds. The elongating cotyledonary petiole pushed the embryo axis out of the seed during germination, which differed from observations in other Quercus species. Shoots emerged from seeds with developing roots after 3 months of warm stratification (35/25 °C), reaching the highest percentage of shoot emergence in seeds after 5 months. Seeds were recalcitrant and displayed a yet unreported epicotyl PD type, for which we propose the formula Cnd(root) ‒ Cp’’ 2b(shoot). Early emergence and development of the root system in Q. chungii seeds with epicotyl PD appears to be a mechanism to maintain a constant water supply to the shoot during plumule development and emergence. Our documentation of seed germination will provide guidance for the conservation and restoration of this species from seeds.

Description: Recently, the Aquaponic Association (AA) published a statement through multiple outlets in response to our article entitled “The Occurrence of Shiga Toxin-Producing E. coli in Aquaponic and Hydroponic Systems.” In this paper, we reported that Shiga toxin-producing Escherichia coli (STEC) was found in fish feces, in the water of both aquaponic and hydroponic systems, and on the surface of the roots of lettuce, basil, and tomato regardless of the system, but not in the edible portions of the plants. Our results demonstrated that the presence of STEC in aquaponics was due to the introduction of contaminated fish that were brought into the system and that cross-contamination might have occurred in the adjacent hydroponic systems during handling events. [...]

Description: This study investigated whether the interaction of protein level and grain type can affect milk production, nutrient digestibility and rumen fermentation in primiparous Holstein cows. Four dietary treatments were used: high-protein with barley as the only grain source, HP-B; (2) high-protein with an equal mix of barley and maize, HP-BM; (3) low-protein with barley as the only grain source, LP-B and (4) low-protein with equal proportions of barley and maize, LP-BM. High-protein diets showed no improvement in milk or protein yield compared with low-protein, but barley and maize mix diets increased energy-corrected milk yield and fat yield compared with barley-only diets. The highest total apparent digestibility of dry matter, organic matter and neutral detergent fibre was observed for LP-BM whereas HP-BM showed the greatest crude protein digestibility. Treatment had no effect on total volatile fatty acid concentrations, molar proportion of acetate and propionate and acetate to propionate ratio. The lowest ruminal pH was observed for LP-B. High-protein diets resulted in greater concentrations of ammonia nitrogen (N), urinary N, blood and milk urea nitrogen compared with low-protein diets, whereas low-protein diets showed better nitrogen utilization efficiency. This study showed that primiparous lactating cows do not benefit from high-protein diets with different fermentation rates of grain sources, but barley and maize diets may improve milk production performance, ruminal fermentation and pH under the present dietary conditions. The current results on milk production performance should be interpreted with caution due to the small number of cows used (eight in each treatment).

Description: Most urban greening interventions involve soil de-sealing and management to enhance fertility. Management typically requires translocating fertile topsoil to the site, which comes at great environmental costs. We hypothesized that de-sealed urban soils would undergo an increase of their fertility without exogenous topsoil application. We assessed experimental plots with de-sealed soil with topsoil, and de-sealed soil without topsoil. Both treatments were vegetated with two ornamental shrub species and irrigated. Soil fertility was analyzed by chemical (total and organic carbon) and biological indicators of soils (biological quality index and microbial activities). Since metal contamination is related to urban de-sealed soil, we also monitored the concentration of Zn, Cu and Pb in soil and detected it in plant leaves. The results demonstrate that de-sealed urban soils rapidly restore their biological quality and fertility. Restoration of de-sealing soils can contribute to the recent growing interest reclamation of urban soils for improving the urban environment quality through the restoration of soil functions and related ecosystem services. Overall, the results of this study demonstrate that de-sealed soils can improve their functionality and can contribute to the recent growing interest in reclamation of urban soils for improving the urban environment quality.

Description: Earthworm activity is a key factor in creating soil aggregates, but introduced organic matter and abiotic factors are also equally important. The purpose of this study was to investigate the stability of aggregates made by earthworms in soils with organic additives. Additionally, the two aggregate stability measurement methods were compared: (i) the wet-sieve method and (ii) the laser diffraction method. A six-month container experiment containing sixteen treatments and controls were made. Each treatment included one of four types of soil texture: sand, loam, silty loam and clay, and one of four additives: straw, peat, compost and compost with added microorganisms. To each treatment, six earthworms were added, two each of species commonly occurring in Polish soils: Dendrodrilus rubidus, Aporrectodea caliginosa and A. rosea. This study confirmed that earthworm activity was the factor favoring aggregate formation. In terms of the investigated organic additives, the efficiency of aggregate creation was as follows: compost with active bacteria, compost, peat and straw. Nevertheless, earthworms alone, without the addition of an organic additive, did not form permanent aggregates. The wet sieving and laser diffractometry methods of measuring aggregate stability were comparable for silty, clayey and loamy soils.

Description: Wilson’s Disease is a rare autosomal recessive disorder in humans, often presenting with hepatic copper overload. Finding the genetic cause of a rare disease, especially if it is related to food constituents like the trace element copper, is a Herculean task. This review describes examples of how the unique population structure of in-bred dog strains led to the discovery of a novel gene and two modifier genes involved in inherited copper toxicosis. COMMD1, after the discovery in 2002, was shown to be a highly promiscuous protein involved in copper transport, protein trafficking/degradation, regulation of virus replication, and inflammation. Mutations in the ATP7A and ATP7B proteins in Labrador retrievers and Dobermann dogs resulted in a wide variation in hepatic copper levels in these breeds. To our knowledge, numerous dog breeds with inherited copper toxicosis of unknown genetic origin exist. Therefore, the possibility that men’s best friend will provide new leads in rare copper storage diseases seems realistic.

Description: Peripheral regions exist in most European countries, and in the countries of the European Union they have been supported for many years by the Community’s Cohesion Policy, which aims at reducing development disparities between the Member States and regions within individual countries. In Poland, five out of sixteen voivodeships in the country are considered to be peripheral regions. They are located on Poland’s eastern border, which is also the eastern border of the European Union. Support programmes for these regions have attained the status of separate operational programmes, focusing on generating convergence effects, which can be achieved by increasing the level of innovation and implementing intelligent ways of operating the economy. The aim of this paper is to demonstrate the essence of peripherality and the innovative-development potential of peripheral regions in Poland, as well as the potential of smart rural development. The paper is based on an analysis of research results drawn from the literature on the subject, as well as from official information and statistics. Own research on the smart village concept in three regions of Eastern Poland was also used. This research showed a reduction in disproportions in the development of regions in Poland, and a link between the level of innovation and the economic growth of the regions, which occurred with a certain lag in relation to the transfer of innovation.

Description: Cassava mosaic disease (CMD) is a major viral disease adversely affecting cassava production in Africa and Asia. Genomic regions conferring resistance to the disease have been mapped in African cassava germplasm through biparental quantitative trait loci (QTL) mapping and genome-wide association studies. To facilitate the utilization of these markers in breeding pipelines to support selections, proof-of-concept technical and biological validation research was carried out using independent pre-breeding and breeding populations. Kompetitive Allele-Specific Polymerase Chain Reaction (KASP) assays were designed from three single nucleotide polymorphism (SNP) markers linked to a major resistance locus on chromosome 12 (S12_7926132, S12_7926163) and a minor locus on chromosome 14 (S14_4626854). The designed assays were robust and easy to score with 〉99% genotype call rate. The overall predictive accuracy (proportion of true positives and true negatives) of the markers (S12_7926132 and S14_4626854) was 0.80 and 0.78 in the pre-breeding and breeding population, respectively. On average, genotypes that carried at least one copy of the resistant allele at the major CMD2 locus had a significantly higher yield advantage. Nevertheless, variation was observed in prediction accuracies for the major locus (S12_7926132) among sub-families from the two populations, suggesting the need for context-specific utilization, for example, by screening for co-segregation of favorable SNP alleles with resistance in the parents being used for crosses. Availability of these validated SNP markers on the uniplex KASP genotyping platform represents an important step in translational genetics toward marker-assisted selection to accelerate introgression of favorable resistant alleles in breeding populations.

Description: Nitrogen (N) management remains a global challenge for the sustainability of diversified farming systems. Field crops are often over-supplied with nitrogen by farmers aiming to high productivity. Although the increase of nitrogen rates leads in many instances to high yields, degree of effectiveness for nitrogen use remains low. Urease and nitrification inhibitors are technologies which have been present in the fertilizers market at least 50 years. Inhibitors exploitation ensures long-term nitrogen release and improved N-uptake by plants and N-storage in seeds and silage. Avail of inhibitors, such as the decline of nitrogen leaching in form of NO3−, reduction of emissions in NH3 form, and rise of yield, are some of the desirable attributes that are derived from their integration in fertilization schedules. This review reports the evaluation of applied nitrogen, with inhibitors, and field crops based on nitrogen indices. The examined N-indicators include Nitrogen use efficiency (NUE), Nitrogen Utilization Efficiency (NUtE,) Nitrogen Agronomic Efficiency (NAE), Nitrogen Harvest Index (NHI), and N uptake. This review gathered all, to the best of our knowledge, available data regarding the utilization of nitrification and urease inhibitors under an exclusively agronomic perspective. Either dual or single use of nitrification and urease inhibitors has been reported to significantly increase yield components and promote nitrogen uptake. To conclude, the assessment of N-related indices is vital to promoting sustainability in diversified farming systems, while the integration of inhibitors in national N fertilizations schemes may contribute to system profitability through enhancement of N-supply to crops.

Description: This study provides data on the environmental sustainability, economic profitability, and gastrointestinal tract development of Siberian sturgeon diets containing black soldier fly full-fat larvae meal (BSFL) for a fish meal (FM) and fish oil (FO) replacement. BSFL was used at 5%, 10%, 15%, 20%, 25%, and 30% of the diet, replacing by up to 61.3% of FM and 95.4% of FO. BSFL positively affected the feed efficiency ratio, and lowered FM and FO usage per kg of fish gain. All the BSFL diets showed a sustainable fish-in fish-out (FIFO) ratio, which was lowered by up to 75% in comparison to the control. Economic assessment per kg of fish gain showed that the most lucrative variants were variants with 10% and 15% BSFL, it finds a mode of action in improvements of the gastrointestinal tract development, including increased pyloric caeca and proximal intestine shares and enhanced villus height and area. Thus, in Siberian sturgeon, BSFL may be used not only as an FM and FO replacer but also as a functional material due to its feed utilization and beneficial health effects, which are reflected in its high sustainability and favourable economics.

Description: The spatial distribution of potentially toxic elements in land near a heavily loaded highway in the West Attica region (Greece) is discussed. This study aimed to investigate the extent of soil contamination with trace elements on land in the margin of highways. The concentration of thirty-five elements in topsoil, car ash, and road sediment bulk samples was determined. Statistical and spatial analysis was applied for evaluating the geochemical dispersion of the examined elements. Geo-accumulation index was estimated. Elements content were compared to criteria and screening values established by the literature. The concentration of As, Cu, Mn, Ni, P, Pb, and Zn in topsoil appeared to be influenced by traffic emissions. It is found that engine exhaust, tyre wear, and brake lining contribute to the As, Ba, Cu, Mn, Ni, P, Pb, and Zn content recorded in topsoil in the margin of highways. The bulk sample of road sediment is moderately contaminated to extremely contaminated with Cu, Mn, Ni, P, Pb, and Zn. Comparison of elements content in topsoil with screening values revealed that Al, As, B, Ba, Cd, Co, Cr, Cu, Fe, Mn, Ni, Sb, V, and Zn pose a potential risk for plants.

Description: Semi-natural grasslands are particularly important in mountainous areas of Romania, being the only source of forage for many farmers. The aim of this study was to investigate the changes in forage quantity and quality as a result of Urea Ammonium nitrate (UAN) liquid fertilization. The experiment was carried out in the eastern part of Apuseni Mountains, Romania on a Festuca rubra L.-Agrostis capillaris L. grassland located at 1240 m altitude. Studies were made over three years of experimental trial (2014–2016) and covered four experimental plots in three replicates, as follows: V1–control plot, unfertilized; V2–plot fertilized with 50 kg UAN ha−1 year−1; V3–plot fertilized with 75 kg UAN ha−1 year−1, and V4–plot fertilized with 100 kg UAN ha−1 year−1. The experimental plots were harvested once per year and the botanical composition, dry matter yield and forage quality were assessed. Our results showed important changes in forage quantity, quality and diversity as a result of UAN fertilization. Starting from the second experimental year the dominance/co-dominance ratio changed favoring the species from Poaceae family. Dry matter increased as a result of UAN fertilization but forage quality was negatively affected by the higher percentage of participation of species from other botanical families which have higher crude fiber content and lower crude protein. Based on our results we recommend moderate fertilization with UAN up to 50 kg UAN ha−1 year−1 for semi-natural grasslands located in soil-climatic conditions similar to those in our experiment.

Description: The Polish agricultural economy has a chance to dynamically develop and influence the innovation policy in the EU model of bioeconomy. The research aims to assess the spatial diversification of the level and structure of spending funds for two Rural Development Program (RDP) measures: agri-environment-climate measures (AECM) and organic farming scheme (OFS) aimed at supporting proenvironmental forms of agricultural management in the context of bioeconomy development. The EU financial perspective determined the time range for 2014–2020. The study was conducted on the example of Poland in two spatial scales: regional (province) and local (community). The analysis was based on partial indicators, which were then subjected to the standardisation procedure and included in the total as a synthetic indicator of the utilisation of RDP 2014–2020 funds aimed at supporting proenvironmental forms of farming. The following information was included in the evaluation: the number of farms, the size of utilised agricultural area (UAA) covered by support and the amounts of payments made under the two analysed RDP measures. In the research, the size and distribution of farms benefiting from AECM and OFS were determined. Besides, the relationship between funds absorption and socioeconomic development, as well as natural and non-natural conditions, were identified. The synthetic indicator of AECM/OFS usage showed a strong spatial differentiation, determined by the impact of several conditions: the level of socioeconomic development, the level of agriculture development, natural conditions of agriculture, land with significant natural and ecological values, and proenvironmental forms of land use on farms. Spatial diversification is more often the result of the impact of proenvironmental or natural-ecological factors than of socioeconomic conditions, or the level of agricultural development.

Description: Forest classifications by disturbance permit designation of multiple types of both old growth forests and shorter-lived forests, which auto-replace under severe disturbance, and also identification of loss of the disturbance type and associated forest. Historically, fire and flooding disturbance regimes, or conversely, infrequent disturbance, produced unique forests such as disturbance-independent forests of American beech (Fagus grandifolia), eastern hemlock (Tsuga canadensis), and sugar maple (Acer saccharum) in the Eastern United States. However, disturbance has changed to primarily frequent mechanical overstory disturbance, resulting in novel forests. To demonstrate the transition to no-analog forests after disturbance change, I compared historical tree surveys (ca. 1837 to 1857) to current surveys in the Northern Lower Peninsula of Michigan. To establish widespread disturbance change effects, I also located where beech and hemlock are currently most abundant throughout the Eastern US compared to historical distribution of beech–hemlock forests. In the Northern Lower Peninsula of Michigan, beech and hemlock historically were about 30% of all trees, but currently, beech and hemlock are 2% of all trees. Red maple (Acer rubrum) increased from 1% to 11% of all trees and aspen (Populus) increased from 2% to 13% of all trees. The squared-chord difference between historical and current surveys was 0.40, or dissimilar forests. Areas with ≥20% beech and hemlock or ≥15% of either species decreased from about 52 million to 6 million hectares, with current distribution restricted to the Northeastern US. Current forests are dissimilar to historical forests, and this transition appears to be driven by disturbance regimes without historical analogs. Disturbance change may provide perspective in forest management for climate change.

Description: According to the United Nations (UN) Refugee Agency, there were 79.5 million forcibly displaced people worldwide by the end of 2019. Evictions from homes and land are often linked to protracted violent conflict. Land administration (LA) can be a small part of UN peace-building programs addressing these conflicts. Through the lens of the UN and seven country cases, the problem being addressed is: what are the key features of fit-for-purpose land administration (FFP LA) in violent conflict contexts? FFP LA involves the same LA elements found in conventional LA and FFP LA, and LA in post conflict contexts, as it supports peace building and conflict resolution. However, in the contexts being examined, FFP LA also has novel features as well, such as extra-legal transitional justice mechanisms to protect people and their land rights and to address historical injustices and the politics of exclusion that are the root causes of conflict. In addition, there are land governance and power relations’ implications, as FFP LA is part of larger UN peace-building programs. This impacts the FFP LA design. The cases discussed are from Darfur/Sudan, Democratic Republic of Congo, Honduras, Iraq, Jubaland/Somalia, Peru and South Sudan.

Description: Assessing transport CO2 emissions is important in the development of low-carbon strategies, but studies based on mixed land use are rare. This study assessed CO2 emissions from passenger transport in traffic analysis zones (TAZs) at the community level, based on a combination of the mixed-use development model and the vehicle emission calculation model. Based on mixed land use and transport accessibility, the mixed-use development model was adopted to estimate travel demand, including travel modes and distances. As a leading low-carbon city project of international cooperation in China, Shenzhen International Low-Carbon City Core Area was chosen as a case study. The results clearly illustrate travel demand and CO2 emissions of different travel modes between communities and show that car trips account for the vast majority of emissions in all types of travel modes in each community. Spatial emission differences are prominently associated with inadequately mixed land use layouts and unbalanced transport accessibility. The findings demonstrate the significance of the mixed land use and associated job-housing balance in reducing passenger CO2 emissions from passenger transport, especially in per capita emissions. Policy implications are given based on the results to facilitate sophisticated transport emission control at a finer spatial scale. This new framework can be used for assessing the impacts of urban planning on transport emissions to promote sustainable urbanization in developing countries.

Description: To solve the water use conflicts between agriculture and ecosystems in arid and semiarid areas, a multi-objective trade-off analysis method was applied to determine the environmental flows (e-flows) for the Yellow River Estuary, by considering the temporal and spatial discrepancies in water allocation. The results showed that during average years, a loss of 3.7 × 108 yuan was caused with every 1 × 108 m3 of e-flows under the baseline scenario. The crop growth stages of April–July are sensitive periods for water requirements, and over 5000 yuan/ha production losses were caused by prioritizing e-flows during this time in dry years. The stages from July–October require more water by ecosystems than other stages, and the recommended e-flows during this time accounted for 57% of the e-flows during the total year. Under scenarios 1–3, which represent the short-term, medium-term and long-term scenarios, more water resources were supplied by underground water and water diversion projects; however, alleviating the water use contradiction remained difficult in dry years. During average years, e-flows between 148 and 168 × 108 m3 are recommended to meet the ecological objectives of survival, reproduction and biological integrity of species for the Yellow River Estuary. The recommended e-flows in wet years could meet higher ecological objectives but still barely achieve the targets of sediment transport and ecosystem dynamic balance. In dry years, the economic losses may be beyond the acceptance of irrigation stakeholders if more water is allocated to improve e-flows. In this case, 71 × 108 yuan would be paid to them to compensate for their losses. This study proposes an e-flow recommendation framework that is economically and ecologically optimal in areas with irreconcilable water-use contradictions.

Description: Under high organic matter content, even under low extractable soil P concentrations, sufficient or over-sufficient foliar P levels may be found. This multi-year study aimed at examining the effects of organic matter content and irrigation management on (a) soil fertility, (b) P-cycle related soil enzymes (acid and alkaline phosphatase, pyrophosphatase) and (c) foliar nutrient concentrations. Irrigated and non-irrigated groves of fully productive trees of the cultivar “Chondrolia Chalkidikis” with low organic matter (LOM 〈 1.5%), medium organic matter (1.5% 〈 MOM 〈 2.5%) and high organic matter (HOM 〉 2.5%) were selected for the experimentation. It was hypothesized that olive groves receiving high inorganic fertilization and irrigation inputs (usually with medium to relatively low organic matter content) would show higher soil and foliar P concentrations compared to the non-irrigated groves with higher organic matter content receiving manure applications. Most of the soil variables (including the three enzymes’ activities) were affected by differences in organic matter content. However, organic matter content did not show a significant influence on foliar nutrient concentrations. Olive trees, especially those cultivated in soils with high organic matter content (receiving organic fertilization), can over-satisfy their P nutritional needs, even though soil analyses show low soil extractable P concentrations, probably due to the high enzymatic activity of acid and alkaline phosphatases. The practical conclusion of this study is that P fertilizer recommendations should be primarily based on foliar P rather than on extractable soil P.

Description: The paper reviews the challenges about haemonchosis—a significant and common parasitic infection of small ruminants. Haemonchus contortus is a highly pathogenic parasite that localises in the abomasum of affected animals and exerts its pathogenicity by blood-sucking activity, adversely affecting the health and productivity of animals. The first challenge is the uneven distribution of the infection globally, this being more prevalent in tropical and subtropical and warm temperate and summer rainfall regions than in cool and cold temperate and arid regions; hence, this leads in differences in the approaches required for its control. Another challenge is the widespread presence of Haemonchus strains resistant to the various anthelmintics available: Benzimidazoles, imidazothiazoles, macrocyclic lactones, closantel and monepantel, which makes the control of the infection difficult. The third challenge refers to the difficulty of diagnosing the disease, given that field evidence can provide suspicion about the infection, which needs to be subsequently confirmed by laboratory tests through parasitological or molecular techniques. The final challenge relates to the difficulties in the control of the infection and the necessity to use pharmaceutical products cautiously and with a planned approach, to avoid further development of anthelmintic resistance, also given that use of a recently licenced vaccine is not widespread. In conclusion, at the moment, we should be concerned, but not worried, about this infection, and apply correctly the appropriate health management plans.

Description: The use of robots is increasing in agriculture, but there is a lack of suitable robotic technology for weed management in orchards. A robotic lawnmower (RLM) was installed, and its performance was studied between 2017 and 2019 in a pear orchard (1318 m2) at Ibaraki University, Ami. We found that the RLM could control the weeds in an orchard throughout a year at a minimum height (average weed height, WH: 44 ± 15 mm, ± standard deviation (SD) and dry weed biomass, DWB: 103 ± 25 g m−2). However, the RLM experiences vibration problems while running over small pears (33 ± 8 mm dia.) during fruit thinning periods, which can stop blade mobility. During pear harvesting, fallen fruits (80 ± 12 mm dia.) strike the blade and become stuck within the chassis of the RLM; consequently, the machine stops frequently. We estimated the working performance of a riding mower (RM), brush cutter (BC), and a walking mower (WM) in a pear orchard and compared the mowing cost (annual ownership, repair and maintenance, energy, oil, and labor) with the RLM. The study reveals that the RLM performs better than other conventional mowers in a small orchard (0.33 ha). For a medium (0.66 ha) and larger (1 ha) orchard, the RLM is not more cost-effective than RM and WM. However, the existing RLM performed weed control well and showed promise for profitability in our research field. We believe that, if field challenges like fallen fruit and tree striking problems can be properly addressed, the RLM could be successfully used in many small orchards.

Description: Antimicrobial resistance (AMR) represents one of the most critical challenges that humanity will face in the following years. In this context, a “One Health” approach with an integrated multidisciplinary effort involving humans, animals and their surrounding environment is needed to tackle the spread of AMR. One of the most common ways for bacteria to live is to adhere to surfaces and form biofilms. Staphylococcus aureus (S. aureus) can form biofilm on most surfaces and in a wide heterogeneity of environmental conditions. The biofilm guarantees the survival of the S. aureus in harsh environmental conditions and represents an issue for the food industry and animal production. The identification and characterization of biofilm-related proteins may provide interesting insights into biofilm formation mechanisms in S. aureus. In this regard, the aims of this study were: (i) to use proteomics to compare proteomes of S. aureus growing in planktonic and biofilm forms in order to investigate the common features of biofilm formation properties of different strains; (ii) to identify specific biofilm mechanisms that may be involved in AMR. The proteomic analysis showed 14 differentially expressed proteins among biofilm and planktonic forms of S. aureus. Moreover, three proteins, such as alcohol dehydrogenase, ATP-dependent 6-phosphofructokinase, and fructose-bisphosphate aldolase, were only differentially expressed in strains classified as high biofilm producers. Differentially regulated catabolites metabolisms and the switch to lower oxygen-related metabolisms were related to the sessile conformation analyzed.

Description: Objective: The health effects of a supplemented Mediterranean diet (SMD) with extra-virgin olive oil (EVOO) and nuts are well documented in non-HIV-infected individuals. We hypothesised that the benefits of an SMD could be mediated by changes in the gut microbiota, even in those with an altered intestinal microbiota such as people living with HIV. Design: Individuals living with HIV (n = 102) were randomised to receive an SMD with 50 g/day of EVOO and 30 g/day of walnuts (SMD group) or continue with their regular diet (control group) for 12 weeks. Methods: Adherence to the Mediterranean diet was assessed using the validated 14-item MD-Adherence-Screener (MEDAS) from the PREDIMED study. A sub-study classifying the participants according to their MEDAS scores was performed. Results: The lipid profile was improved in the SMD group vs. that in the control group (delta-total cholesterol and delta-B-lipoprotein). The immune activation (CD4+HLADR+CD38+ and CD8+HLADR+CD38+ cells) and IFN-γ-producing T-cells significantly decreased at week 12 compared to the baseline in the SMD group but not in the control group. The gut microbiota in those from the high-adherence group presented significantly high diversity and richness at the end of the intervention. Succinivibrio and Bifidobacterium abundances were influenced by the adherence to the MD and significantly correlated with Treg cells. Conclusion: The Mediterranean diet improved metabolic parameters, immune activation, Treg function, and the gut microbiota composition in HIV-1-infected individuals. Further, Mediterranean diet increased the Bifidobacterium abundances after the intervention, and it was associated to a beneficial profile.

Description: One hundred and eighty Anglo-Arabian horses running 1239 races were sampled for the present study. DNA was extracted from the blood and myostatin gene, MSTN, was genotyped. Moreover, prizes won and places were achieved for the 1239 races to perform association analyses between the different genotypes and sport traits. Two SNPs already reported in previous studies regarding the Thoroughbred breed, rs69472472 and rs397152648, were revealed as polymorphic. The linkage disequilibrium analysis investigating the haplotype structure of MSTN did not evidence any association block. Polymorphism at SNP rs397152648, previously known as g.66493737 T〉C, significantly influenced sport traits, with heterozygous horses TC showing better results than homozygotes TT. The portion of variance due to the random effect of the individual animal, and the other phenotypic effects of sex, percentage of Arabian blood and race distance, computed together with the genotype at MSTN in the statistical models, exerted a significant influence. Hence, this information is useful to improve knowledge of the genetic profile of Anglo-Arabian horses and a possible selection for better sport performance.

Description: Contraception is an important population control method for the colony management of primates housed in captivity. Etonogestrel (ENG) implants (i.e., Implanon®) are a widely used progestin-based contraceptive in common marmosets (Callithrix jacchus) with the theoretical advantages of being reversible and long-acting. However, no dose and efficacy data are available yet. Therefore, data from 52 adult female marmosets contracepted with ENG (one-fourth or one-third of an implant) housed at the Biomedical Primate Research Centre (BPRC, Rijswijk, The Netherlands) over the past 18 years were analyzed. Using an electronic database, a retrospective longitudinal cohort study was conducted to calculate the reproductive data before, during and after ENG use. The data show an effectiveness in preventing pregnancy of 99%. The implant was effective within one week after insertion. Unintended pregnancies did occur, but in 60% of these cases, the animals were already pregnant at the time of implant insertion. In these cases, healthy offspring were born despite the use of the implant. No stillbirths, neonatal deaths or maternal deaths could be linked to ENG use. After implant removal, 83% of the animals delivered healthy offspring. No difference in contraception efficacy was observed between the use of one-fourth or one-third of an implant. ENG achieved a contraceptive protection exceeding 99% and was shown to be reversible concerning fertility. To our knowledge, this is the first detailed analysis on the use of ENG in marmosets.

Description: The rheological properties of wood–plastic composites (WPCs) with different wood fiber contents were investigated using a rotational rheometer under low shear rates. The flow field information was analyzed and simulated by Ansys Polyflow software. The results showed that the WPCs with different wood fiber contents behaved as typical power-law fluids. A higher wood fiber content increased the shear thinning ability and pseudoplasticity of the WPCs. The pressure, velocity, shear rate, and viscosity distributions of the WPC during extrusion could be predicted by computational fluid dynamics (CFD) Ansys Polyflow software to explore the effects of different components on the flow field of WPCs.

Description: Background: Pasteurized donor milk (PDM) is typically prescribed to preterm or low birthweight newborns when their mother’s own milk is unavailable. In surplus, PDM is prescribed to meet the nutritional needs of healthy newborns in the first few days of life. However, its overuse can undermine efforts to promote and support breastfeeding, waste resources, and reduce the availability of PDM for at-risk newborns. We conducted this study to examine factors associated with the prescription and prolonged use (〉48 h) of privately purchased PDM to healthy newborns. Methods: Prospective observational study of 2440 mothers of healthy, term, and normal birthweight newborns born at Da Nang Hospital for Women and Children between April and August 2019. In addition to the descriptive analysis, we performed multiple logistic regressions to examine factors associated with the prescription of PDM (n = 2440) and prolonged PDM use among those who used PDM (n = 566). Results: Twenty-three percent (566/2440) of healthy, term, and birthweight ≥2500 g newborns received PDM and were included in the study. The prevalence of PDM use was higher for cesarean births (OR: 2.05; 95% CI: 1.66, 2.55) and among male newborns (OR: 1.33; 95% CI: 1.09, 1.62), but lower for farmers or workers (vs. other jobs; OR: 0.71; 95% CI: 0.54, 0.93), family income

Description: Vitamin D has been hypothesized to play an important role in preventing the development and progression of periodontal disease, but the underlying immune modulatory mechanisms remain understudied. We examined the cross-sectional association between biomarkers of vitamin D status and C-reactive protein (CRP) among postmenopausal women aged 53–81 years. Linear regression was used to examine the association between plasma 25-hydroxyvitamin D (25[OH]D) concentrations, a biomarker of vitamin D status, and both salivary and serum CRP concentrations in 567 women from the Buffalo Osteoporosis and Periodontal Disease (OsteoPerio) Study (1997–2000). CRP concentrations were measured with multiplex arrays and transformed for normality using the natural log. Concentrations above and below the limit of detection were included in analysis as right- and left-censored observations. An inverse association was observed between 25(OH)D and salivary CRP in a model adjusted for age, smoking status, frequency of tooth brushing and flossing, and hormone therapy use (−7.56% difference in salivary CRP concentrations per 10 nmol/L increase in 25(OH)D, 95% CI: −12.78 to −2.03). Further adjustment for percent body fat attenuated this association (−2.48%, 95% CI: −7.88 to 3.24). No significant associations were found between 25(OH)D and serum CRP. Plasma vitamin D concentrations were not associated with salivary or serum CRP concentrations in this cohort of postmenopausal women.

Description: The gold-spotted pond frog (Pelophylax chosenicus) is an endangered amphibian species in South Korea. In order to obtain ecological information regarding the gold-spotted pond frog’s habitat environment and biological interactions, we applied stable isotope analysis to quantify the ecological niche space (ENS) of frogs including black-spotted pond frogs (P. nigromaculatus) and bullfrogs (Lithobates catesbeianus) within the food web of two different habitats—an ecological wetland park and a rice paddy. The gold-spotted pond frog population exhibited a broader ENS in the ecological wetland park than in the rice paddy. According to the carbon stable isotope ratios, gold-spotted pond frogs mainly fed on insects, regardless of habitat type. However, the results comparing the range of both carbon and nitrogen stable isotopes showed that gold-spotted pond frogs living in the rice paddy showed limited feeding behavior, while those living in the ecological wetland park fed on various food sources located in more varied trophic positions. Although the ENS of the gold-spotted pond frog was generally less likely to be overlapped by that of other frog species, it was predicted to overlap with a high probability of 87.3% in the ecological wetland park. Nevertheless, gold-spotted pond frogs in the ecological wetland park were not significantly affected by the prey competition with competitive species by feeding on other prey for which other species’ preference was low. Since these results show that a habitats’ food diversity has an effect on securing the ENS of gold-spotted pond frogs and prey competition, we recommend that the establishment of a food environment that considers the feeding behavior of gold-spotted pond frogs is important for the sustainable preservation of gold-spotted pond frogs and their settlement in alternative habitats.

Description: The development of social production and the agglomeration of the urban population have brought tremendous pressure to transportation infrastructure. However, the impacts of transportation development on urban land use systems have not been well investigated. Under the pressure of limited land resources, the impact of transportation infrastructure on urban land use efficiency (ULUE) is receiving increasing attention from scholars and needs to be explored. By collecting panel data from 30 regions in China from 2003 to 2018, in this study we constructed a spatial Durbin model and a panel threshold regression model to explore the spatial spillover effects and threshold effects of transportation infrastructure on ULUE. The most obvious findings emerging from this study are that (1) ULUE is not randomly distributed over different regions in China, but has an obvious positive spatial correlation; (2) transportation infrastructure has significant positive direct and spatial spillover effects on ULUE and the direct effects of transportation infrastructure (0.823) are significantly stronger than the spatial spillover effects (0.263); (3) the impact of transportation infrastructure on ULUE has a significant double threshold effect, and the threshold values are 4.520 and 6.429 respectively, and with the improvement of transportation infrastructure, its marginal effects on ULUE show a downward trend. This paper provides theoretical support for policymakers to achieve cross-regional cooperation on land use and transportation infrastructure construction and inspirations for sustainable development.

Description: Planetary boundaries are an important sustainability concept, defining absolute limits for resource use and emissions that need to be respected to avoid major and potentially irreversible earth system change. To remain within the safe operating space for humanity, there is a need for urgent adoption of climate-neutral diets, which make no additional contribution to warming. In the first study of its kind, a new climate metric, the Global Warming Potential Star (GWP*), was used to assess greenhouse gas (GHG) emissions associated with 9341 Australian adult diets obtained from the Australian Health Survey. Dietary climate footprints averaged 3.4 kg CO2-equivelent per person per day, with total energy intake explaining around one quarter of the variation. Energy-dense and nutrient-poor discretionary foods contributed around one third. With lower climate footprint food choices, a diet consistent with current Australian dietary guidelines had a 42% lower climate footprint. Currently, it is not possible to define a climate-neutral dietary strategy in Australia because there are very few climate-neutral foods in the Australian food system. To bring Australian diets into line with the climate stabilization goals of the Paris Agreement, the most important need is for innovation across the agricultural and food processing industries to expand the range of climate-neutral foods available.

Description: A few prospective studies have suggested that tea, alcohol, and fruit consumption may reduce the risk of kidney stones. However, little is known whether such associations and their combined effect persist in Chinese adults, for whom the popular tea and alcohol drinks are different from those investigated in the aforementioned studies. The present study included 502,621 participants from the China Kadoorie Biobank (CKB). Information about tea, alcohol, and fruit consumption was self-reported at baseline. The first documented cases of kidney stones during follow-up were collected through linkage with the national health insurance system. Cox regression was used to calculate the hazard ratio (HR) and 95% confidence interval (CI). During a median of 11.1 years of follow-up, we collected 12,407 cases of kidney stones. After multivariable adjustment, tea, alcohol, and fruit consumption were found to be negatively associated with kidney stone risk, but the linear trend was only found in tea and fruit consumption. Compared with non-tea consumers, the HR (95% CI) for participants who drank ≥7 cups of tea per day was 0.73 (0.65–0.83). Compared with non-alcohol consumers, the HR (95% CI) was 0.79 (0.72–0.87) for participants who drank pure alcohol of 30.0–59.9 g per day but had no further decrease with a higher intake of alcohol. Compared with less-than-weekly consumers, the HR (95% CI) for daily fruit consumers was 0.81 (0.75–0.87). Even for those who did not drink alcohol excessively, increasing tea and fruit consumption could also independently reduce the stone risk. Among Chinese adults, tea, alcohol, and fruit consumption was associated with a lower risk of kidney stones.

Description: There is growing evidence for a global transition to a more highly processed diet. While the dietary share of ultra-processed foods depends on a country’s economic status, food choice and consumption are also influenced by the socioeconomic situation of individuals. This study investigated whether ultra-processed food consumption differed across socioeconomic subgroups and over time (2010–2018) in Korea. Cross-sectional data from the Korea National Health and Nutrition Examination Survey 2010–2018 were analyzed. Food and beverages reported in a one-day 24 h recall were classified according to the NOVA food classification criteria. The dietary energy contribution of ultra-processed foods was high among men and urban residents, and increased with education and income level; additionally, it reached its peak in adolescents and thereafter decreased with increasing age. After adjusting the socioeconomic variables, such associations remained significant, except for income level. The overall contribution of ultra-processed foods increased from 23.1% (2010–2012) to 26.1% (2016–2018), and the same trend over time was observed in all age groups and socioeconomic strata. In the Korean population, ultra-processed food consumption differed by individual socioeconomic characteristics, but gradually increased over time, and this trend was consistently found in all socioeconomic subgroups. Future strategies to promote healthy food choices are needed for the Korean population.

Description: Soil spatial variability mapping allows the delimitation of the number of soil samples investigated to describe agricultural areas; it is crucial in precision agriculture. Electrical soil parameters are promising factors for the delimitation of management zones. One of the soil parameters that affects yield is soil compaction. The objective of this work was to indicate electrical parameters useful for the delimitation of management zones connected with soil compaction. For this purpose, the measurement of apparent soil electrical conductivity and magnetic susceptibility was conducted at two depths: 0.5 and 1 m. Soil compaction was measured for a soil layer at 0–0.5 m. Relationships between electrical soil parameters and soil compaction were modelled with the use of two types of neural networks—multilayer perceptron (MLP) and radial basis function (RBF). Better prediction quality was observed for RBF models. It can be stated that in the mathematical model, the apparent soil electrical conductivity affects soil compaction significantly more than magnetic susceptibility. However, magnetic susceptibility gives additional information about soil properties, and therefore, both electrical parameters should be used simultaneously for the delimitation of management zones.

Description: Enterospora nucleophila is a microsporidian enteroparasite that infects mainly the intestine of gilthead sea bream (Sparus aurata), leading to an emaciative syndrome. Thus far, the only available information about this infection comes from natural outbreaks in farmed fish. The aim of the present study was to determine whether E. nucleophila could be transmitted horizontally using naturally infected fish as donors, and to establish an experimental in vivo procedure to study this host–parasite model without depending on natural infections. Naïve fish were exposed to the infection by cohabitation, effluent, or intubated either orally or anally with intestinal scrapings of donor fish in four different trials. We succeeded in detecting parasite in naïve fish in all the challenges, but the infection level and the disease signs were always milder than in donor fish. The parasite was found in peripheral blood of naïve fish at 4 weeks post-challenge (wpc) in oral and effluent routes, and up to 12 wpc in the anal transmission trial. Molecular diagnosis detected E. nucleophila in other organs besides intestine, such as gills, liver, stomach or heart, although the intensity was not as high as in the target tissue. The infection tended to disappear through time in all the challenge routes assayed, except in the anal infection route.