Publication Date:
2002-06-22
Description:
Positive-strand RNA viruses such as poliovirus replicate their genomes on intracellular membranes of their eukaryotic hosts. Electron microscopy has revealed that purified poliovirus RNA-dependent RNA polymerase forms planar and tubular oligomeric arrays. The structural integrity of these arrays correlates with cooperative RNA binding and RNA elongation and is sensitive to mutations that disrupt intermolecular contacts predicted by the polymerase structure. Membranous vesicles isolated from poliovirus-infected cells contain structures consistent with the presence of two-dimensional polymerase arrays on their surfaces during infection. Therefore, host cytoplasmic membranes may function as physical foundations for two-dimensional polymerase arrays, conferring the advantages of surface catalysis to viral RNA replication.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lyle, John M -- Bullitt, Esther -- Bienz, Kurt -- Kirkegaard, Karla -- AI-42119/AI/NIAID NIH HHS/ -- New York, N.Y. -- Science. 2002 Jun 21;296(5576):2218-22.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Microbiology and Immunology, Stanford University School of Medicine, Stanford, CA 94305, USA.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/12077417" target="_blank"〉PubMed〈/a〉
Keywords:
Base Sequence
;
Binding Sites
;
Catalysis
;
Crystallography, X-Ray
;
HeLa Cells
;
Humans
;
Hydrogen-Ion Concentration
;
Inclusion Bodies, Viral/metabolism/ultrastructure
;
Microscopy, Electron
;
Models, Molecular
;
Molecular Sequence Data
;
Mutation
;
Nucleic Acid Conformation
;
Poliovirus/*enzymology/physiology
;
Protein Conformation
;
Protein Structure, Quaternary
;
Protein Structure, Tertiary
;
RNA Replicase/*chemistry/isolation & purification/*metabolism/ultrastructure
;
RNA, Viral/biosynthesis/*metabolism
;
Viral Core Proteins/metabolism
;
Virus Replication
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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