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  • American Institute of Physics  (27,145)
  • Blackwell Publishing Ltd  (9,321)
  • Public Library of Science
  • 1965-1969  (36,466)
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  • 101
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    Metroeconomica 21 (1969), S. 0 
    ISSN: 1467-999X
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Economics
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  • 102
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    Metroeconomica 21 (1969), S. 0 
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  • 103
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    Metroeconomica 21 (1969), S. 0 
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  • 104
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    Metroeconomica 21 (1969), S. 0 
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  • 105
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    Metroeconomica 21 (1969), S. 0 
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  • 106
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    Metroeconomica 21 (1969), S. 0 
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  • 107
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    Metroeconomica 21 (1969), S. 0 
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  • 108
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    Metroeconomica 21 (1969), S. 0 
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  • 109
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    Metroeconomica 21 (1969), S. 0 
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  • 110
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    Metroeconomica 21 (1969), S. 0 
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  • 111
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    Metroeconomica 21 (1969), S. 0 
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  • 112
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  • 113
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    Metroeconomica 21 (1969), S. 0 
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  • 114
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    Metroeconomica 21 (1969), S. 0 
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  • 115
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    Metroeconomica 21 (1969), S. 0 
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  • 116
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    Metroeconomica 21 (1969), S. 0 
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  • 117
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    Metroeconomica 21 (1969), S. 0 
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  • 118
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    Metroeconomica 21 (1969), S. 0 
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  • 119
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    Metroeconomica 21 (1969), S. 0 
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  • 120
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    Metroeconomica 21 (1969), S. 0 
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  • 121
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    Metroeconomica 21 (1969), S. 0 
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  • 122
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    Metroeconomica 21 (1969), S. 0 
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  • 123
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    Ground water 7 (1969), S. 0 
    ISSN: 1745-6584
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geosciences
    Notes: New sanitary landfills are being opened each day to accommodate the rising volume of solid wastes. Selection of proper sites is a very major part of the disposal problem, particularly as they might affect the surrounding surface and ground water.Leachate production is inevitable in the humid East and throughout most of the country. Several alternatives are suggested that would result in the assimilation of migrating leachate into, the environment at tolerable limits or for its renovation prior to final discharge: Alternative 1—knowledge of existing hydrogeologic conditions which would favorably control the rate and direction of leachate migration; Alternative 2—engineering the landfill by construction of low-cost facilities to collect and treat leachate; and Alternative 3—construction of limited collection facilities to supplement natural conditions.It is suggested that more emphasis be given to hydrogeologic factors in the selection of refuse disposal sites to insure protection of surrounding surface and ground water. It is time to approach the waste disposal problem on a more sophisticated level by requiring test drilling, monitoring, and by giving consideration to the engineering of, landfills so that man's health and' environment are not jeopardized.
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  • 124
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    Ground water 7 (1969), S. 0 
    ISSN: 1745-6584
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geosciences
    Notes: A simple and rapid method of determining casing length and permeable zones in wells tapping bedrock can be useful to well drillers and hydrologists. A device consisting of a galvanometer, a reel of insulated wire, and a copper electrode locates the casing depth, changes of lithology, and permeable zones. The small-diameter electrode permits measurement through well-seal access ports avoiding the expensive and time-consuming procedure of removing the seal and drop pipes to measure casing depth with a magnet. The measured electromotive force changes rapidly when the electrode passes the end of casing. Thus, the depth of casing is easily determined from the length of wire payed out. Changes in electromotive force measured within the uncased part of a well frequently indicate permeable zones, thereby aiding in choice of the most efficient drop-pipe length, and also yielding useful information for hydrogeologic studies.
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  • 125
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    Ground water 7 (1969), S. 0 
    ISSN: 1745-6584
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geosciences
    Notes: The best features of analog and digital computers were combined to make a management model of a stream-aquifer system. The analog model provides a means for synthesizing, verifying, and summarizing aquifer properties; the digital model permits rapid calculation of the effects of water-management practices. Given specific management alternatives, a digital program can be written that will optimize operation plans of stream-aquifer systems. The techniques are demonstrated by application to a study of the Arkansas River valley in southeastern Colorado.
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  • 126
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    Ground water 7 (1969), S. 0 
    ISSN: 1745-6584
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geosciences
    Notes: Underground detonations may produce observable effects in surrounding aquifers and wells. The nature and the duration of the effect at any observation point seem to depend on several factors such as the amount of energy released by the detonation, the geologic environment, the position of the buried explosive device in relation to the saturated zone, aquifer characteristics, and the distance from point of detonation. Precise measurement of these effects in wells presented numerous technical problems and resulted in the development of specialized techniques. Initially, these effects were observed by measuring the fluctuation of the free water surface in wells. The current technique employs high-resolution pressure transducers deep in the water column. Pneumatic packers may be used to restrict the movement of water into the well. Data are recorded on high-speed oscillographs.
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  • 127
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    Ground water 7 (1969), S. 0 
    ISSN: 1745-6584
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geosciences
    Notes: Present ground-water use in Ohio, approximately 650mgd (million gallons per day) amounts to about 5 percent of the water that enters the ground-water reservoirs. The largest ground-water supplies are developed where natural concentrations of water occur, chiefly in the watercourse aquifers, which consist of sand and gravel of glacial origin (outwash) in the valleys of the major streams. Other important aquifers are glacial outwash in upland areas and in the buried Teays Valley system, the limestone and dolomite aquifers in western Ohio, and sandstone and shale aquifers in the eastern half of the State.Future outlook is that more of the increasing water demand will be met from ground-water sources. Ground-water supplies will be developed at many new sites, and aquifers in areas already heavily pumped will be made to yield more water by the drilling of additional wells and recharging the aquifers artificially. Large quantities of ground water in storage, virtually unexploited, could be used for temporary low-flow augmentation of streams. Management of ground-water resources will be needed to help solve supply and distribution problems, and to resolve conflicts between users. Among future problems will be those arising from underground disposal of wastes, a practice which is expected to grow substantially from enforcement of water-quality standards for streams, set under the Federal Water Quality Act of 1965.
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  • 128
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    Ground water 7 (1969), S. 0 
    ISSN: 1745-6584
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geosciences
    Notes: Pumping test data from a single well were used to estimate aquifer volume affecting drawdown. After four days of pumping from a 240 feet thick welded tuff aquifer in southern Nevada, the volume of the wedge-shaped mass tested was estimated to be about 15.6 billion cubic feet (440 million cubic meters). The field coefficient of permeability of the aquifer is 275 gallons per day per square foot.
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  • 129
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    Ground water 7 (1969), S. 0 
    ISSN: 1745-6584
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geosciences
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  • 130
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    Ground water 7 (1969), S. 0 
    ISSN: 1745-6584
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geosciences
    Notes: In Southern California's San Bernardino Valley a unique experiment is being performed by a local water district, the State government, and a private industry. Both the experiment and the organizational arrangements for getting the job done are the subject of this paper. The experiment is the application of systems analysis technology to water resource management. The organization is formed by a combination of contracts and cooperative agreement between private and government agencies. The result is a favorable environment for the development of effective water resource management strategies.The San Bernardino Valley is similar Co many areas in the world where water resource management reduces to the allocation of locally available ground water and potential imported water resources. This paper outlines in detail the analysis tools and long-range planning needs of effective ground-water management strategies.
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  • 131
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    Ground water 7 (1969), S. 0 
    ISSN: 1745-6584
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geosciences
    Notes: Coal mining in Appalachia has degraded both the surface and ground water. During mining, ground water is drained from the rocks and the pyrite associated with the coal beds is exposed to air. Oxidation of the pyrite produces high iron ana sulfate concentration and a low pH in the water. Some of this polluted water flows directly into nearby streams and some moves into the ground-water system. When the latter occurs, the iron concentration can increase up to several hundred mg/1 and the sulfates to over one thousand mg/1. Unfortunately, in most cases the cessation of mining does not stop the ground-water pollution, and it can take many decades before the ground water again becomes usable.A detailed study of the effects of coal mining on ground water was conducted in the Toms Run drainage basin in northwestern Pennsylvania where coal mining and oil and gas well drilling have occurred for almost 100 years. The rocks of Mississippian and Pennsylvanian Age produce a multiaquifer system–three major aquifers separated by siltstone and shale beds (aquitatdes). The oil and gas wells act as a conduit system permitting acid mine drainage to move downward from the strip mines to underlying aquifers. It then moves laterally down dip and discharges as springs. The acid mine drainage adversely affects the ground-water quality by increasing the iron and sulfate content of the water especially in the vicinity of the strip mines.
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  • 132
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    Ground water 7 (1969), S. 0 
    ISSN: 1745-6584
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geosciences
    Notes: The measure of earth resistivity is a possible means of detecting and outlining zones of ground-water contamination where a resistivity contrast exists between contaminated and unconcaminated ground water.As a preliminary evaluation of the use of electrical resistivity for defining zones of contaminated ground water, five sites on Long Island and three sites in western Texas were examined. The surveys at three of the Long Island sites and at one of the western Texas sites were at least partially successful in their objectives. The lack of success at the other sites is attributed to the particular physical conditions that existed.Further effort toward developing electrical resistivity as a method for evaluating variations in ground-water quality is encouraged.
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  • 133
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    Ground water 7 (1969), S. 0 
    ISSN: 1745-6584
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geosciences
    Notes: Water-supply problems in Spain have become acute during the past decade owing to expansion of industry, construction of modern houses and apartments, and development of a tourist industry. A training program in ground-water hydrology and geology was established three years ago in Barcelona to help supply personnel who would be able to cope with these problems. Training is also given to foreign nationals, a total of nine countries having been represented to date. Part of the success of the school rests on the fact that a large variety of ground-water problems exist in the vicinity of Barcelona. Students obtain a firsthand knowledge of sea-water intrusion, aquifer-testing techniques, agricultural and municipal degradation of water quality, and drilling and exploration techniques within a wide variety of rock types.
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  • 134
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    Ground water 7 (1969), S. 0 
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    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geosciences
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  • 135
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    Ground water 7 (1969), S. 0 
    ISSN: 1745-6584
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geosciences
    Notes: A well-designed and calibrated orifice meter is an accurate and inexpensive measuring device for flow. Endline orifices can be calibrated at work sites by solving an equation that interrelates easily measured dimensions of the orifice and outflow.
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  • 136
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    Ground water 7 (1969), S. 0 
    ISSN: 1745-6584
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geosciences
    Notes: Expanding water requirements during recent years have intensified development of the productive sedimentary aquifers of the North Carolina Coastal Plain. The effects of this development emphasize the local and regional limitations of the aquifer system and the need for an effective management program.Withdrawal of about 60 million gallons per day from the Castle Hayne limestone, the most productive unit of the principal artesian aquifer of the area, began in July 1965 at a phosphate mine adjacent to the Pamlico River in Beaufort County. Within a few months, the artesian head was lowered below sea level in an area of about 800 square miles, and to more than 100 feet below sea level in the immediate vicinity of pumping. Under these conditions the aquifer became vulnerable to encroachment of saline water from (1) areas of natural occurrence of brackish water in the limestone member of the aquifer; (2) leakage of brackish surface water through the confining beds; and (3) vertical movement of brackish water from the underlying sand member of the aquifer.The dramatic and “overnight” effects of the large withdrawals at the mine site and the threat of damage to the aquifer created sufficient public concern so that legislation was passed by the 1967 General Assembly, giving the Board of Water and Air Resources authority to regulate ground-water withdrawals as needed to protect the aquifers of the State. An adequate management program can be accomplished that will not only protect the aquifer but will improve the development potential. The development of such a management program requires a thorough knowledge of the hydrology of the aquifer system, the objective of studies in progress. The implementation of the program will require the support of an informed public.
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  • 137
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    Ground water 7 (1969), S. 0 
    ISSN: 1745-6584
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geosciences
    Notes: The planned development of an individual water supply from a group of wells must take into consideration several controllable and uncontrollable factors. The uncontrollable factors may include aquifer coefficients of transmissibility and storage, aquifer boundaries, static water level, aquifer depth, recharge capabilities, and competing users. The controllable factors often include the acreage allocated to ground-water development, well locations, and pumping rates. Well-field design is oriented mainly toward proper selection of the controllable factors.Water requirements, the cost of water and the life of the well field are related to limitations of acreage, draw-down of water level, and investment in pumping and transmission facilities. All of these factors, in turn, are influenced by the location of individual wells with respect to one another.Well-field design calculations involve the combined interference of water level drawdown among the various wells in the field. Repeated computational trials for various numbers of wells and well spacings may be required. Calculations using conventional methods are, in most cases, rather time-consuming.The final design decision can be approached quickly if it is assumed that the wells have regular spacing. Through the use of a simple formula and table developed in this paper, a given well-field problem can be calculated in a few minutes with a slide rule. When well spacing deviates appreciably from the regular spacing assumed, the final design calculations should be made with computer techniques.
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  • 138
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    Ground water 7 (1969), S. 0 
    ISSN: 1745-6584
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geosciences
    Notes: This article contains a summary of a few disease out-breaks caused by ground-water contamination, and the difficulties in designing and monitoring for effective quality control. There is a discussion of problems associated with large basin recharge with treated sewage. For instance, do we have adequate microbial indicators? Or will the nitrate concentration build up if there is a semiclosed circuit involved? Chlorination of well water withdrawn for domestic use is advocated as good insurance for microbial control.
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  • 139
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    Ground water 7 (1969), S. 0 
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    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geosciences
    Notes: A new design method for deep well dewatering systems is given. The method analyses three dimensional flow to the multiwell systems by considering the two end points to the initial fast phase of water table fall. The method is applied to a dewatering case history and proves to be successful.
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  • 140
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    Ground water 7 (1969), S. 0 
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  • 141
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    Ground water 7 (1969), S. 0 
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    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Geosciences
    Notes: A new method of determining aquifer storage coefficients has been developed for use in operations involving ground-water recharge through wells. This work evolved from a research project dealing with the fate of DDT and nitrate in the Ogallala Aquifer in the High Plains of Texas.A tritium tracer was used to determine the detention time of the recharge water passing through a saturated volume of the aquifer. Tracer behavior was formulated ‘mathematically and verified by model simulation. The formulations provide a graphical solution for the detention time from which the storage coefficient may be calculated.The method will have immediate value in establishing storage coefficients of aquifers, such as the Ogallala, where plans for importation and distribution of supplemental water involve utilization of underground storage. Regarding water-table conditions, this method promises to produce more reliable storage values than have been obtained from pumping tests.
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  • 142
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    Journal of food science 34 (1969), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: —The activities of tyrosinase, invertase, trypsin and chymotrypsin were studied after exposure to one or more of the following high-pressure inert gases: N2O at 600 psig or N2 or Ar each at 5000 psig. Exposure to high-pressure nitrogen or argon failed to significantly inhibit the rate of tyrosinase activity in fluid systems. However, the rates of tyrosinase-catalyzed reactions in shell-cast gelatin gels were significantly depressed by exposure to high-pressure nitrogen, and even more so by high-pressure nitrous oxide. This inhibition proved to be oxygen dependent and reversible. Pressurization experiments with invertase, trypsin and chymotrypsin indicated that high-pressure N2O did not significantly inhibit these enzymes. This lends support to the hypothesis that high-pressure inert gases inhibited tyrosinase activity in nonfluid systems by decreasing the availability of oxygen, rather than by physically altering the enzyme. It must be concluded that there is little hope that the enzymes in food systems can be effectively inhibited by brief exposure to inert gases at pressures of 5000 psig or less.
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  • 143
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: SUMMARY– Proteolysis and its relationship to tenderness were studied by measuring nonprotein nitrogen (NPN), free amino groups, and shear resistance during post-mortem aging of bovine muscle. Both NPN and free amino groups increased during post-mortem aging, indicating some degradation of proteins and/or peptides. However, neither the increase in NPN nor free amino groups was related to post-mortem tenderization since these quantities increased only after most of the improvement in tenderness had occurred. Much of the increase in NPN or free amino groups may originate from degradation of sarcoplasmic proteins or peptides. It is suggested that weakening or breaks at crucial points in the sarcomere, such as at the junction of the Z-line with the thin filaments, occur within the first 48-72 hr post-mortem and that this weakening or cleavage is responsible for tenderization. Cathepsin D may be responsible for this weakening but most of the available evidence is against proteolysis as the primary cause of post-mortem tenderization.
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  • 144
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    Journal of food science 34 (1969), S. 0 
    ISSN: 1750-3841
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: SUMMARY– The principal tannin of sorghum is a leuco-anthocyanin yielding luteolinidin (3′,4′,5,7-tetrahydroxy-flavylium) when heated with mineral acid. The precursor, luteoforol, has most of the properties of 3′,4,4′,5,7-penta-hydroxyflavan prepared by reduction of eriodictyol. Luteoforol, when treated with concentrated mineral acid in the cold, gives a purple color with Λmax550nm. A method for the determination of luteoforol in sorghum, based on this property, is described. The results with a number of varieties of sorghum are compared with those obtained by the AOAC Folin-Denis method. The contribution of luteoforol to the “tannin” so determined varies from 1 to 〈 25%. Except for one sample of Kaffir corn, which contained leucocyanidin as well as luteoforol, no other tannins were detected. The “tannin” content varied widely, (from 0.05 to 0.67% as tannic acid), a white-skinned variety having the least. The uniformity of commercial samples can be rapidly evaluated by single-grain determinations of luteoforol.
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  • 145
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: SUMMARY— A method that uses SPS agar and incorporates an improved egg yolk agar and nitrate motility medium has been developed for the enumeration and confirmation of vegetative cells and/or spores of Clostridium perfringens in foods. The method is based upon several diagnostic criteria and can be completed within 48 hr.
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  • 146
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: –The relative sweetness of sugars and sugar mixtures was studied. In addition to the simple sugars (sucrose, dextrose and fructose), the amino acids, glycine and D, L-alanine, and the synthetic sweeteners, calcium cyclamate and sodium saccharin, were studied. Using the method of magnitude estimation, considerable data were obtained about relative sweetness over a reasonably wide concentration range. Only two sessions per subject were required to obtain meaningful results. Relative sweetness of the sugars was found to increase with increasing concentration—a pattern quite similar for all the sugars. Changing the reference or reference concentration resulted in shifts in the relative sweetness values for a particular sugar; however, these changes were consistent at all concentrations tested. Slope values for the individual sugars were in good agreement with previously reported results. The individual subjects responses showed a consistent pattern throughout the 10-month period.Synergistic effects, as much as 20 to 30%, were noted in several sugar mixture combinations but not all concentrations. The data support the concept that there are optimal mixture combinations. The potential applications of these observations are discussed.
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  • 147
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: SUMMARY— Organoleptic and chemical deterioration of freshwater whitefish muscle frozen at −10°C for periods up to 16 weeks was assessed. As frozen storage of muscle progressed, the toughness and rancidity of baked muscle increased. The solubility of the myofibrillar protein fraction, “actomyosin,” dropped from about 72 to 22% over the 16 week storage period of whitefish muscle. No change in the solubility of sarcoplasmic protein in frozen stored muscle was observed. However, with polyacrylamide disc electrophoresis, two new sarcoplasmic protein bands were detected after 16 weeks of storage. With storage of frozen muscle, water-binding capacity diminished. Although the total lipid and cholesterol contents of muscle remained constant throughout frozen storage, the phospholipid content decreased as the free fatty acid content increased. Oxidative deterioration of lipid in frozen muscle was estimated.
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  • 148
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: SUMMARY— Experiments with cooked, freeze-dried beef support previous evidence that changes in its physical and chemical properties are reflected in its water sorption characteristics. As a new approach, sorption isobars and their hysteresis were studied. In the reported temperature and pressure range, a decrease in the sorption capacity and in the area of the hysteresis loop was observed after 1 yr of storage.
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  • 149
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: SUMMARY: A completely randomized design was followed to evaluate 48 roasts posterior third of the loin) from 12 Duroc and 12 Poland China barrows. Antemortem treatment of pigs produced “normal”, pale-soft-exudative (PSE) and dark-firm-dry (DFD) longissimus dorsi (LD) muscle. Meat was roasted at 350°F to an internal temperature of 167°F and evaluated by organoleptic and selected objective measurements. There was no significant organoleptic preference for one type (“normal”, PSE, DFD) of LD. Also, differences among types of muscle were not significant for roasting time, volume of press fluid or total moisture (press method); whereas roasting losses, total moisture (Brabender) and pH of LD were affected significantly by type of muscle. In general, there were significant (P 〈 0.05) differences between PSE and DFD muscle, and between “normal” and DFD muscle. DFD muscle exhibited the smallest roasting loss and greatest total moisture, whereas PSE muscle had the greatest roasting loss and least total moisture. DFD muscle rated highest in pH and lowest in Warner-Bratzler shear value. LD from Durocs was more tender (P 〈 0.05), had a higher (P 〈 0.05) pH, and contained less (P 〈 0.01) total moisture (Brabender) than LD from Poland Chinas. Cooking losses were greater (P 〈 0.05) for roasts from Durocs than for roasts from Poland Chinas.
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  • 150
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: SUMMARY: Bisulfite retarded the inactivaton of horseradish peroxidase by weak acids. Spectral analysis indicated that the retardation was accomplished by the stabilization of the linkage between the iron containing prosthetic group and the protein. Cyanide, azide and fluoride, which form reversible complexes with peroxidase iron, exerted a similar effect; thus, it is inferred that bisulfite also forms a complex with peroxidase iron. A kinetic method was used to calculate a dissociation constant of 0.2M for the bisulfite peroxidase complex.
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  • 151
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: SUMMARY: Direct gas chromatographic vapor analyses were utilized to determine whether banana slices at different stages of ripeness in an in vitro system produced iso-amyl acetate and iso-amyl alcohol, known banana aroma constituents; and whether a precursor-product relationship could be observed between these compounds and L-leucine. Production of iso-amyl acetate by unripe slices was demonstrated based on experiments with metabolic inhibitors. The behavior of ripe and overripe slices was inconclusive since the vapor concentration of the acetate remained constant. The vapor concentration of iso-amyl alcohol was essentially unchanged at all ripeness stages. Investigations with L-leucine-U-14C showed conclusively that leucine was a precursor and that both compounds were continually produced at all ripeness stages. The interpretation of direct vapor analyses over respiring fruit is considered.
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  • 152
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: SUMMARY: Survivor curves for spores of Bacillus subtilis were determined in wet and dry heat over a wide range of temperatures. Wet heat tests were determined using a thermoresistometer and thermal death time cans. Dry heat tests were conducted in a closed system using thermal death time cans. There were major differences in the shape of the wet vs. dry heat survivor curves. Wet heat resulted in convex curves at low temperatures, but a straight line at higher temperature. Dry heat resulted in concave curves at all temperatures. These results suggest that physiological differences exist between wet and dry heat destruction of bacteria. Several possible explanations for the difference in the shape of the survivor curves were discussed.
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  • 153
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    Journal of food science 34 (1969), S. 0 
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: SUMMARY: Steaks from 24 bulls 20 months old, 89 bulls 14 months old and 109 steers 20 months old were used to relate thickness of epimysial tissue to the amount of intra-muscular connective tissue, and to correlate these factors with Warner-Bratzler shear values in muscles cooked to 140 or 160°F. The thickest portion of the LD epimysium was greater in lighter weight bulls than it was in heavier steers. Epimysial extension and thickness varied according to muscle location but at the same location it was not significantly different in steaks from tough versus tender steers. Differences in hydroxyproline content and connective tissue of steers and young bulls were small but older bulls had slightly greater amounts of both. The LD epimysium was tougher in older bulls than in younger bulls.Shear values of epimysial tissue varied considerably within animals of the same age, especially when roasted to 140°F. Most connective tissue differences due to line of cattle were small. Correlations within groups of cattle between thickness of epimysial tissues of different muscles or between epimysial thickness and intramuscular connective tissues were low. With few exceptions neither hydroxyproline, epimysial thickness nor connective tissue scores were significantly related to shear force of muscle or shear force of epimysial tissue itself. Correlations between shear force of the LD and BF muscles were not significant (P 〈 0.05) in animals of the same age. There was a tenderness interaction between age of animal and type of muscle which suggested that collagen gelatinization occurred at a faster rate in the LD than in the BF as age increased.
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  • 154
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: SUMMARY: The changes in polygalacturonase (PG) activity during ripening and the relationship between its activity and the quality of Deglet Noor dates were studied. PG activity, which was virtually absent at the green stage, began to develop as maturity progressed. The greatest part of the development occurred at the late red stage and activity reached its maximum when the fruits commenced softening. Among the four grades of dates used in this study the activity was highest with natural, followed by waxy, number 1 dry and number 2 dry grades. The dates contained 0.9-1.3 units of PG per g of fresh tissue. The results of chemical and physical analyses related to the texture are also reported.
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  • 155
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: SUMMARY: 28 pigs weighing approximately 100 lb were dosed with 10,000 to 15,000 excysted trichina larvae, grown to 285 lb and sacrificed. Fresh lean samples from one ham and shoulder per carcass were digested in a pepsine-HCI solution and microscopically examined to determine infection rates. 24 of the more heavily infected hams and shoulders then were selected for further observations. The hams and shoulders were dry-cured for 2 days per lb, using an 8% cure applied in 3 equal applications at 5-day intervals. The cure contained salt, sugar, potassium nitrate and sodium nitrite. After curing, the meat was placed in salt equalization for 30 days, smoked for 24 hr at 100°F and aged at 75°F until termination of the study. Cored lean samples were taken from 8 hams and shoulders at weekly intervals after salt equalization and analyzed for salt concentrations and viability. Viable trichinae persisted throughout curing, salt equalization, smoking and 2 weeks of aging. Samples taken from hams and shoulders after 3 weeks of aging were found to be free of any live trichinae. Similar lean samples were taken at this time and force fed to rats for 5 days. After 8 weeks on a commercial ration the rats were sacrificed, artificially digested and examined. No trichina larvae could be recovered.
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  • 156
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: SUMMARY: This research was undertaken in order to determine the relationship between the phenomena of meat swelling (SW), viscosity (η), extract-release volume (ERV), and wafer-holding capacity (WHC) as simple and rapid techniques of determining beef microbiol qualify. These techniques were compared by analyzing beef samples held from freshness to spoilage at refrigerator temperatures, fresh beef homogenized with urea, and beef adjusted to different pH values. In addition, irradiated fresh ground beef samples were inoculated with the normal flora of spoiled beef and analyzed similarly.With the microbially spoiled meats the highest degree of correlation occurred between SW and ERV, followed by ERV & WHC, SW & η, η & ERV and SW & WHC. These findings indicate that ERV and SW are quite similar and equally reliable in determining meat microbiol qualify while the relationships between η and ERV, and η & WHC were of lower orders of significance.When fresh beef was homogenized with urea at levels between 0.5 and 5M, η correlated best with SW followed by ERV & η, and ERV & SW. Over the pH range 3-11, SW and ERV correlated best followed rather closely by SW and η, and ERV & η.
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  • 157
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: SUMMARY: Autoxidation of methyl linoleate dispersed on filter paper was studied in the dry state in the presence and absence of additives. Oxidation was followed volumetrically and by measuring the increase in conjugated dienes. Evaluation of the rate data indicates that added histidine is antioxidant in the very early stages of the oxidation and becomes prooxidant in the later stages. Increasing the concentration of histidine promoted an earlier appearance of its prooxidant effect. Cobalt chloride and manganese sulfate had a pronounced catalytic effect on the oxidation reaction in the model systems buffered to a high (8.0 and 9.0) but not to a low (4.0) pH. Histidine increased the catalytic activity of manganese but eliminated that of cobalt. Thin-layer chromatography showed that conditions under which histidine increased the prooxidant effect of manganese also resulted in the appearance of some decomposition products of histidine. These decomposition products may have catalytic activity.
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  • 158
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: SUMMARY: Volatile components of commercial Montmorency cherry essence boiling above ethanol were extracted from the essence by ether and concentrated by distillation. This concentrate was fractionated by gas chromatography. Individual components were identified using the methods of functional group analysis, gas co-chromatography, infrared analysis and mass spectrometry. The concentrations of the components in the original essence were estimated. The major components identified were n-propyl alcohol, isobutyl alcohol, isoamyl alcohol, and benzaldehyde. Minor components identified include n-butanol, n-hexanol, a hexenol, benzyl alcohol, α-terpineol, furfural, isoprene, myrcene and numerous higher terpenes, methyl benzoate, ethyl benzoate, benzyl acetate, ethyl caprylate, ethyl caprate, n-propyl benzoate, isobutyl benzoate, isoamyl benzoate and di-butyl phthalate.
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  • 159
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: SUMMARY: The physical properties of the major muscles of the round from 30 crossbred beef and 20 straightbred dairy carcasses were studied. Sex, sire and body type were related to these properties. Generally, the steers had longer, heavier and lighter-colored muscles than the heifers. A negative relationship was noted between the specific gravity and reflectance values for the crossbred muscles. The semimembranosus muscle was heaviest and accounted for the highest percent of the rough round in certain beef and dairy sire groups. In the dairy cattle, the weight of the biceps femoris muscle was also affected by sire. Heritability estimates indicated that the weight of the semimembranosus was highly heritable and its percent of the rough round moderately heritable in both beef and dairy cattle. The dairy b. femoris weight and circumference were also moderately heritable. Color reflectance, specific gravity, pH, length and circumference/length ratio were not observed to be heritable physical properties.
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  • 160
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: SUMMARY: Death kinetics of spores of Clostridium botulinum, Type A, strain 62, were studied at sterilant gas temperatures in the range of 40°C (104°F) to 70°C (158°F). Hygroscopic carriers in the form of small discs of filter paper were used. The sterilant gas used was a mixture of dichlorodifluoromethane and ethylene oxide (88 and 12% by weight respectively). Pressures in the exposure atmosphere were adjusted to obtain an ethylene oxide concentration of 700 mg per liter at all temperatures. It was shown by gas-chromatographic analysis that an actual concentration of 700 ± 20 mg per liter was maintained at all exposure temperatures during all exposure periods. The effect of various moisture levels on spore death kinetics was also studied. The relative humidities employed in both the preconditioning and exposure of spores were 3, 23, 33, 53 and 73%, respectively. Thermochemical resistance parameters, D and z, were calculated as the reciprocals of the slopes of the survivor curves and thermochemical destruction time curves, respectively. Of the various environmental moisture levels studied, a preconditioning and exposure relative humidity of 3% for destruction of C. botulinum spores taken from an aqueous suspension, was found to be most effective. This effect held for all temperatures studied. Over the range studied, temperature was seen to have the greatest effect at 3% relative humidity (RH), second greatest at 33% and least effect at 73%.
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  • 161
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: SUMMARY: DDT incorporated into chicken tissues during the growing period was reduced in concentration during cooking by either baking, frying, or steaming and during heating of tissues in closed containers for varying lengths of time. DDT was converted to DDD in each of the treatments, but the concentration of DDE was not altered significantly. Total losses of residue were greater when tissues were fried or steamed than when the samples were either baked or heated in closed containers. Losses of residue from chicken tissue occurred primarily through leaching of fat during the cooking process.
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  • 162
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    Notes: SUMMARY: Pig sarcoplasmic reticulum fragments obtained from the longissimus dorsi muscle at 0- and 24-hours post-mortem were purified by salt extraction and density gradient centrifugation. The calcium uptake activity of 0-hour purified preparations was more than 20-fold higher than that from 24-hr old muscles, but there was no significant difference between fractions for calcium activated ATPase activities. When observed electron microscopically after negative staining, the ultrastructures of the 0. and 24-hour membrane fragments were found to be essentially identical. Incubation of isolated sacroplasmic reticulum fragments at pH 7.2 and 37°C or pH 5.6 and 0°C caused negligible inhibitoin of their calcium accumulating ability. However, treatment at pH 5.6 and 37°C for 1 hr almost completely abolished the sarcoplasmic reticulum calcium uptake. Thus it appears that low muscle pH and high temperature may be responsible for the inactivation of the calcium accumulating ability of the sarcoplasmic reticulum that occurs in situ.
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  • 165
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: SUMMARY: The effects of muscle tenderness classification and of aging muscle postmortem on ATPase activity and superprecipitation of natural actomyosin were studied. Actomyosin from muscle 12- and 24-hr postmortem had higher ATPase activity than that from 0-hr, 5-day aged or 10-day aged muscle. However, ATPase activity did not usually return to the 0-hr level. No consistent differences were found in actomyosin ATPase activity after the various periods of aging for actomyosins from tough and tender muscle. Superprecipitation of actomyosin was used as a measure of contractility. Actmyosin from 12- and 24-hr postmortem muscle superprecipitated faster than that from 0-hr muscle. However, actomyosin from 5 and 10-day aged muscle superprecipitated less rapidly than that from 12- and 24-hr postmortem muscle. Superprecipitation was more rapid in actomyosin from tough muscle than tender muscle at low KCI concentrations, but this was not true at high KCI concentrations. This observation suggested that actomyosin from tough muscle had a stronger interaction or higher amounts of some protein factor such as α-actinin than did tender muscle.
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  • 166
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: SUMMARY: Effects on body nitrogen gains of supplying nonessential nitrogen as glutamic or aspartic acids, as glutamine or asparagine or as wheat were investigated. It was found that nitrogen gains of rats fed diets containing only purified amino acids as the nitrogen source and relatively high levels of amide nitrogen were significantly lower than those of rats fed the same amounts of total nitrogen, all as α-amino nitrogen. However, they were significanty higher than those of rats fed the same amounts of α-amino nitrogen, but no additional amide nitrogen, indicating some effect of amide nitrogen in body nitrogen storage. Rats fed ad libitum consumed more of the glutamine-containing diet than of one containing isonitrogenous amounts of glutamic acid; analysis of covariante indicated that ad libitum-fed rats also utilized the nitrogen of glutamic acid more efficiently than that of glutamine. Addition of enough sodium bicarbonate to neutralize the hydrochlorides of dietary amino acids had no effect on utilization of amide nitrogen. No differences in nitrogen utilization were found between two groups of rats fed different levels of amide nitrogen as wheat.
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  • 167
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: SUMMARY: Fat of raw poultry, separated from solid tissue and washed with water at temperatures not exceeding 40°C does not contain cooked poultry aroma and does not develop it when heated. Hence, cooked poultry aroma cannot be derived from the fat alone. Washed and filtered fat from cooked poultry contains characteristic cooked poultry aroma dissolved in it. The aroma of this fat is caused by compounds which dissolve in it during cooking and which apparently stem from nonfat or lean portions of the meat. The ability of fat to dissolve or acquire substances during cooking was demonstrated by showing that fat of cooked poultry contains more sulfur than does fat of raw poultry. Less than 2% of this sulfur build-up occurs as hydrogen sulfide. The magnitude of the sulfur build-up was 8 to 14 times greater in the fat of roasted poultry than it was in the fat of simmered chicken. Authentic amino acids in contact with poultry fat at a typical roasting temperature readily underwent Strecker type degradation. Furthermore, aroma components representing typical amino acid degradation products were found in fat from roasted turkey. These analytical results indicate protein, amino acids and probably also sugars and other water soluble components are invoved in aroma formation. Hence fat contributes to cooked poultry aroma indirectly and passively through its ability to dissolve and retain aroma components formed during cooking. Consequently, the characteristic cooked poultry aroma in fat of cooked poultry is not derived from the fat itself but comes from and is thus dependent on the “lean”.
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: SUMMARY: The activity of glutamic-oxaloacetic transaminase (GOT) and glutamicpyruvic transaminase (GPT) of bovine and porcine muscle tissue and muscle press juice was determined. The total GPT activity of muscle tissue is about one tenth of the GOT activity. There are no remarkable differences in the activities of GOT and GPT between these slaughter animals and other species (rat, rabbit and man). The GOT activity of the longissimus dorsi muscle of pigs is significantly higher than that of the same bovine muscle. The mitochondrial (GOTM) and sarcoplasmic isozymes (GOTB) of GOT in skeletal muscles of cattle and pigs were determined after electrophoretic separation. The ratio GOTM:GOTS in skeletal muscle was found to be about 1:1. There is only a small decrease in GOT activty during storage of muscle tissue at 0 or +4°C for several weeks postmortem. The small activity of GOTM in the muscle press juice does not substantially change during storage of muscle tissue under these conditions, indicating that there is no drastic change of the mitochondrial structure during aging of meat. Bacterial spoilage of meat, however, results in the release of GOTM from the mitochondria.
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: SUMMARY: Freezing and thawing of bovine and porcine muscle cause a remarkable release of the mitochondrial isozyme of the glutamic-oxaloacetic transaminase (GOTM) from the mitochondrial structures resulting in an increase of GOTM activity in the muscle press juice. The lower the freezing temperature the stronger is this effect. Repeated freezing and thawing increases the release of GOTM. It is considered that the level of GOTM activity in the muscle press juice indicates the extent of mitochondrial damage. On the basis of these results a simple and rapid routine method was developed which allows a reliable differentiation between nonfrozen and frozen and thawed meat.
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: SUMMARY: Mitochondrial and lysosomal preparations from rat livers were examined for the presence of a number of particulate bound enzymes, previously described as being present in mitochondria. The study sought to determine whether these enzymes were truly mitochondrial or partly associated with lysosomes which are contaminants of most mitochondrial preparations. The amino transferases were of particular interest because of their possible involvement in the metabolism of amino acids which are concentrated within lysosomes. It was shown that particulate aspartate amino transferase and alanine amino transferase are truly mitochondrial enzymes. Particulate glutathione reductase was shown to be distributed between lysosomes and mitochondria, the specific activities in the two particles being almost equal. Possible activation of catheptic protein hydrolysis is discussed. Lysosomes were shown not to contain the following enzymes: isocitrate dehydrogenase, glycerol-3-phosphate dehydrogenase, serine dehydratase, glucose-dphosphate dehydrogenase, xanthine oxidase, aldehyde oxidase and fumarate hydratase. Lysosomes were unable to incorporate amino acids into proteins but were able to incorporate acetate 14C into fatty acids. The importance of this process as a possible mechanism for controlling or altering the structure of the lysosomal membrane is discussed. Relationships of these findings to some properties of meats are discussed.
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
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  • 172
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Muscle from stress-susceptible and stress-resistant pigs was studied for capillary distribution and fiber characteristics. More capillaries were associated with red fibers than white fibers and there was a greater capillary to fiber ratio in red than white muscle. There was no difference in capillary fiber ratio between normal (from stress-resistant pigs) and PSE (from stress-susceptible pigs) muscle. Muscle from stress-susceptible animals had more intermediate fibers than did muscle from stress-resistant animals. Certain intermediate fibers from stress-susceptible animals had a high amylophosphorylase and ATPase activity. It was postulated that such fibers are important in the drastic post-mortem muscle behavior in stress-susceptible animals and may represent the characteristic which causes muscle to become PSE.
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  • 173
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The epimysial connective tissues from normal and PSE longissimus dorsi muscles were subjected to physical characterization. No differences in conventionally determined shrinkage temperatures were observed. However, differential thermal analysis (DTA) studies revealed that epimysium from PSE tissues had lower onset and recovery temperatures and contained a higher percentage of components melting at low temperatures than that from normal tissues. The epimysium from PSE muscles also had a higher initial moisture and lower dry matter content. Epimysial connective tissues underwent osmotic swelling in neutral solutions, with those from PSE muscles imbibing significantly more water than that from normal muscles. Dry matter content decreased during neutral swelling, probably due to a loss of ground substance. Collagen from PSE muscles had a molecular weight between cross-links (Mc) of 6.37 ± 104 and a resultant cross-link density of 5.23/ molecule, while normal tissues had corresponding values of 4.67 ± 104 and 7.73. These differences were only significant at the 10% level.
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  • 174
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: SUMMARY– An exhaustive chloroform extraction of maple syrup removed the maple flavorants. The extract was analyzed in part by a gas chromatograph-mass spectrometer tandem procedure. Several previously undetected flavor-related compounds were found in trace amounts. Among these were the aromatic compounds acetovanillone, guaiacyl acetone and vanilloyl methyl ketone. These aromatics could have resulted from the ethanolysis of ligneous material previously reported in maple sap. Sugar degradation products found were furfural, hydroxymethylfurfural, lactic acid and levulinic acid. These indicate that the products of caramelization also are part of the maple flavorants.Acids found, in addition to those above, were the C5 to C9 aliphatic acids and oxalic, fumaric and malic acids. All of the acid occurred as ethyl esters resulting from unintentional esterification during extraction. The C, to C, acids may be artifacts perhaps derived from the vegetable oil used as antifoaming agent in syrup processing.
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  • 175
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: SUMMARY– The epimysial connective tissue from normal and PSE (pale, soft and exudative) porcine muscle was chemically characterized. Samples from PSE muscles exhibited markedly lower turbidity values (P 〈 0.01) than those from normal muscles. No significant differences were found in ultimate pH values. The epimysium from PSE muscles contained significantly higher (P 〈 0.05) quantities of salt soluble tropocollagen and a greater amount of heat labile collagen (P 〈 0.01) than that from normal tissues. No significant differences in α- and β-subunit composition, plasma hydroxy-proline levels or amino acid composition were evident between the two sources of epimysial connective tissues.
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  • 176
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    Notes: SUMMARY– Myofibrillar, mitochondrial, heavy sarcoplasmic reticulum and light sarcoplasmic reticulum fractions were isolated by differential centrifugation of homogenates from normal and pale, soft, exudative (PSE) porcine muscle at various times post-mortem. Calcium uptake was measured using a solution containing45Ca++. The oxalate-stimulated calcium accumulating ability of the subcellular fractions declined 5-10 fold between 0 and 24 hr post-mortem. The major portion of this decline occurred in the first hour after death in fractions from PSE muscle but was more gradual in the normal fractions. The ATPase activities of normal and PSE fractions obtained at death did not differ significantly. These activities increased with time post-mortem in most normal fractions but decreased in those from PSE muscle. The subcellular site of ATP hydrolysis post-mortem was discussed. The results obtained point to the potential importance of the relaxing, factor in muscle post-mortem.
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  • 177
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: SUMMARY— The comparative rates of IMP degradation between fresh and frozen-and-thawed (slacked) fish were compared on six different species of fish. Several factors that could contribute to a rate change of IMP degradation were evaluated. These included freezing temperatures, time in frozen storage, pre- and post-rigor freezing, and method of killing the fish.English sole and rainbow trout showed slight increases in the rate of IMP degradation when they were frozen and then thawed within 48 hr. Silver salmon and halibut that were frozen and then thawed within 48 hr showed no change in the rate of IMP degradation. Halibut, however, that was frozen and stored at −20°F for 3 months showed a slight decrease in the rate of IMP degradation after it was thawed; but king salmon handled under the same conditions did not.The method of kill or freezing the fish either pre- or post-rigor did not alter the rate of IMP degradation after the fish was thawed.No loss of IMP occurred in fish (halibut) stored at −20°F. Over one-third of the original IMP content was lost in halibut stored at +15°F after 3 months of storage.These results show that there is no significant difference in the rate of IMP degradation between fresh and slacked fish. The flavor-contributing effect of IMP in slacked fish therefore should be the same as in fresh fish, provided the fish was frozen and stored at or near a temperature of −20°F.
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  • 178
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: SUMMARY– Analysis was made of the fatty acid composition of liver, adipose fat, thigh and breast muscles of broilers fed corn-soy commercial-type of diets containing one of two levels of fish oil (2.5 or 5.0%). The oil was subsequently continued, withdrawn or replaced with yellow grease 2, 3 or 4 weeks before termination of the experiment at the 8th week. The tissue contents of four w-3-type fatty acids (20:4, 20:5, 22:5 and 22:6) were increased in relation to the number of weeks menhaden fish oil was included in the diet. (The first number indicates the chain length of carbons, the second indicates the number of double bonds. The number following w (omega) indicates the carbon position of the first double bond as counted from the terminal methyl group.)Of the four tissues studied, the liver had the highest total content of the w3 fatty acids; the adipose fat, the least. The muscles had intermediate values; the breast containing more than the thigh. Withdrawal of fish oil at the 4th week resulted in favorable organoleptic evaluation. A more favorable evaluation was obtained if yellow grease was substituted in place of fish oil for the last 4 weeks. The organoleptic scores were highly significantly correlated with the content of 20:5 03, 22:5 w3 and 22:6 w3 but it was less significantly correlated with the content of 20:4 w3. An inverse relation existed between the content of the w3 with that of the w6 fatty acids.
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  • 179
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    Notes: SUMMARY– We studied oxidation of a freeze-dried model system consisting of methyl linoleate and gelatin by incubating the model system in air at 50°C for up to 10 days in the dry state or at controlled relative humidities. Incubation for 5-10 days caused a drop in the viscosity of gelatin solutions, an increase in the solubility of gelatin in ethanol-rich solvent mixtures, an increase in the retention time of gelatin on a Sephadex G-150 column, and a reduction in the melting point of a standard gelatin gel. There were no such changes in the viscosity and solubility properties of gelatin when incubation was at a relative humidity of approximately 60%. In some instances, incubation at high relative humidity led to partial insolubilization of gelatin in water or in acetate buffer. The oxidation effects in the dry state were consistent with the hypothesis that gelatin undergoes oxidative degradation. The effects of oxidation showed similarities to effects of ionizing radiations.
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: SUMMARY– Sarcolemmae are usually identified solely by morphological characteristics. We have determined several chemical and enzymic properties of sarcolemmae from chicken breast muscle prepared by homogenization of aged muscle in dilute CaCl2 solution, washing 4 times in NaCl-histidine at pH 7.4, extraction with water buffered to pH 7 and isolation by differential centrifugation on a discontinuous sucrose gradient. The phospholipid content of the sarcolemmae was low, representing only 2 to 3% by weight compared with the 20 to 35% usually found in membraneous systems. This discrepancy may be due to the relatively small proportion of plasma membrane in the sarcolemma.Analyses indicate little contamination by nuclei or mitochondria. The sarcolemmae have, like the microsomal fraction, high contents of RNA and glucose-6-phosphatase activity. The sarcolemma is either rich in these elements or is contaminated by other subcellular elements, such as the transverse tubules (T system), which are. The sarcolemmae display a Mg+2- activated ATPase activity which is typical for membraneous systems. Lactate dehydrogenase was shown to be associated with the sarcolemmae. Whether this represents the situation in vivo or is an artifact of preparation is not clear. The sarcolemmae are capable of binding soluble LDH.
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  • 182
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: SUMMARY— The lipid composition of Baltic herring (Clupea harengus var. membranus) muscle and the hydrolysis during freeze storage was studied with thin-layer chromatography. The concentrations of the various lipid classes were initially 2–5 times as high in dark muscle as in white muscle. Storage for up to 12 weeks at –15°C resulted in an increase of the free fatty acid content from 50 to 1000 mg/100 g in the dark muscle, and from 17 to 280 mg/100 g in white muscle. The increase was due to hydrolysis of lecithin, cephalin and to a varying extent also of triglycerides. According to indirect estimations, roughly 45 and 75% of the free fatty acid formed in dark and white muscle respectively was a result of phospholipid hydrolysis; the remainder of triglyceride hydrolysis. The hydrolysis of lecithin was faster than that of cephalin in both types of muscles. The enzymatic attack showed no preference for anyone of the different fatty acids in the phospholipids.
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: SUMMARY— Radiopasteurization is an effective means of preserving haddock fillets of varying marketable quality. While results show that the postirradiation shelf life was a function of the preirradiation quality, they also indicate that there was an economic benefit from irradiating the fillets cut from fish as old as 13 days after catching.
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  • 184
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: SUMMARY— Chromatographic estimations of tyramine and histamine in “Marmite” and four other yeast extracts showed a tyramine content from 0.1 to 1.6 mg/g and a histamine content of 0.2 to 2.8 mg/g. “Marmite” contained the largest amounts of both amines, but’ three samples of this extract showed a wide range in amine content. These variations in amine content of yeast extracts are discussed in terms of their manufacture. Pharmacological activity in one of the yeast extracts (“Marmite”) on the rat's blood pressure and on the intact bronchioles and isolated ileum of the guinea pig was due to the presence of tyramine and histamine. The clinical relevance of a high histamine content is discussed.
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Process Engineering, Biotechnology, Nutrition Technology
    Notes: SUMMARY— Volatile components of three varieties of tomatoes processed in plain tin and enamel can linings at 0, 1, 4 and 8 months storage time were determined. A recently developed extraction method was used for separation and collection of volatiles free of moisture. This method allowed the study of small quantities of materials in a reasonable time. Multiple sampling of the vapor aliquots permitted qualitative and quantitative analysis.The greatest qualitative and quantitative differences in volatile composition were noted between the raw and heat processed samples. Methyl sulfide which was absent in the fresh fruit was formed during the heat process. The concentrations of acetaldehyde, acetone, methanol, and hexanal were significantly altered by processing. Differences between varieties were due to varying quantities of individual constituents but quantitative differences between can lining were less apparent.
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    Notes: SUMMARY— The effect of 28 microorganisms on the accumulation of hydroperoxides and monocarbonyls in fresh fat has been determined. Pseudomonas ovalis, Micrococcus freudenreichii and two strains of Streptomyces increased the concentration of peroxides; M. freudenreichii also produced an increase in 2-enals, 2, 4-dienals, and alkanals. Pseudomonas fragi, Geotrichum candidum, and Candida lipolytica produced an increase in alkanals and methyl ketones. All other cultures either removed the small amount of peroxides and monocarbonyls in fresh fat and prevented any build-up or had no effect. The ability of microorganisms to oxidatively attack fats was not related to their oxidase reactions or their ability to produce lipase. Differences in oxidative activity on fresh and rancid lard are discussed.
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    Annals of the New York Academy of Sciences 157 (1969), S. 0 
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    Topics: Natural Sciences in General
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