ISSN:
1750-3841
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
SUMMARY– The outer sheath, or sarcolemma, of the muscle cell plays an important role in the contractile process and may, therefore, be significant in the changes which occur in muscle post-mortem. It has been suggested that a breakdown in a cytoskeleton of the cell is necessary before the contractile proteins become accessible to water and extractable.One procedure for preparing sarcolemmae involves homogenization of the muscle in CaCl2 solution, washing with NaCl-histidine, and an incubation for 30 min at 37°C. Several factors which govern the ability to prepare sarcolemmae from chicken breast muscle largely free of other cellular components have been studied in this report. These include CaCl2 concentrations during homogenization, NaCl and histidine concentrations during the washes, the pH of the wash solutions, the number of washes required, the necessity of the incubation, and the pH of the extracting water.The purpose of these experiments was to find a procedure which would allow variation of the preparatory conditions so that the association of enzymes with the sarcolemma could be studied. It is known that the association of enzymes with particulate fractions of the cell are sensitive to environmental conditions. A necessary step to allow restrictive conditions of preparation, such as low pH and ionic strengths, to be used was aging of the whole excised muscle for 4 hr at 0–4°C. The function of this aging process is not known. It does not appear to involve solubilization of protein nor major changes in several classes of phosphorus compounds.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2621.1969.tb00911.x
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