Publication Date:
1980-09-19
Description:
Transfection of cultured monkey kidney cells with recombinant DNA constructed with a cloned Escherichia coli gene that codes for xanthine-guanine phosphoribosyltransferase and several different SV40 DNA-based vectors, results in the synthesis of readily measurable quantities of the bacterial enzyme. Moreover, the physiological defect in purine nucleotide synthesis characteristic of human Lesch-Nyhan cells can be overcome by the introduction of the bacterial gene into these cells.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Mulligan, R C -- Berg, P -- New York, N.Y. -- Science. 1980 Sep 19;209(4463):1422-7.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6251549" target="_blank"〉PubMed〈/a〉
Keywords:
Animals
;
Cells, Cultured
;
Cloning, Molecular/methods
;
DNA, Bacterial/*genetics
;
*DNA, Recombinant
;
Escherichia coli
;
*Genes
;
Haplorhini
;
Humans
;
Hypoxanthine Phosphoribosyltransferase/genetics
;
Lesch-Nyhan Syndrome/*genetics
;
Pentosyltransferases/*genetics
;
Simian virus 40/genetics
;
Transduction, Genetic
;
Transformation, Genetic
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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