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  • Triticum aestivum  (105)
  • Inversion
  • Saccharomyces cerevisiae
  • Springer  (171)
  • 3
  • Nature Publishing Group (NPG)
  • Wiley
  • 1980-1984  (171)
  • 1
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    Springer
    Cellular and molecular life sciences 40 (1984), S. 1159-1161 
    ISSN: 1420-9071
    Keywords: Saccharomyces cerevisiae ; 5-trifluoromethyl-6-àzauracil ; yeast cell cultures ; cell division ; inhibition of
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Cell division, as studied in asynchronous cultures of yeast cells, is sensitive to 5-trifluoromethyl-6-azauracil (F3CAzU). Under defined conditions (10 mmoles l−1 F3CAzU) this compound blocks immediately and completely the process of cell division. Using synchronized cells, the time-point at which division process of yeast cell can be inhibited by F3CAzU has been determined. The inhibitor effect of this compound is completely reversed by thymine, thymidine and uracil.
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  • 2
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    Current genetics 8 (1984), S. 559-566 
    ISSN: 1432-0983
    Keywords: DNA repair ; Saccharomyces cerevisiae ; Cloning
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Three overlapping plasmids were isolated from a YEp24 library, which restore Rad+ functions to rad6-1 and rad6-3 mutants. Different subclones were made and shown to integrate by homologous recombination at the RAD6 site on chromosome VII, thus verifying the cloned DNA segments to be the RAD6 gene and not a suppressor. The gene resides in a 1.15 kb fragment, which restores Rad+ levels of resistance to U.V., MMS and γ-rays to both rad6-1 and rad6-3 strains. It also restores sporulation ability to rad6-1 diploids. Integrative deletion of the RAD6 gene was shown not to be completely lethal to the yeast. Our results suggest that the RAD6 gene has some cell cycle-specific function(s), probably during late S phase.
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  • 3
    ISSN: 1432-0983
    Keywords: α-Pheromone-inactivating glycoproteins ; bar1-1 ; Barrier proteins ; Purification ; Saccharomyces cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two kinds of a-mating-type-specific proteins inactivating α pheromone (α factor) were purified from heat shock extract of MATa cells. Their molecular weights were estimated to be 400,000 and 200,000 by gel filtration. Both proteins were detected in MATa SST1 cells but not in MATα SST1, MATa sst1-1 and MATa/MATα SST1/SST1 cells. In addition, the proteins were detected in matα2-1 SST1 cells but not in matα1-2 SST1 cells. From these results, it is concluded that these proteins are synthesized under the control of the SST1 gene and responsible for the Barrier action of MATa cells. The relationship of these proteins to the secreted Barrier protein having a higher molecular weight is discussed.
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  • 4
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; TRP3 gene ; Deletion analysis ; Enzyme function
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two sets of deletions, entering the TRP3 gene of Saccharomyces cerevisiae from the 3′- and the 5′-end were constructed. Complementation analysis with chromosomal trp3A, trp3B and trp3C mutations was done by introducing the 3′- and 5′-truncated gene on a multicopy 2 μm-vector. The N-terminal glutamine amido transferase function is encoded by a DNA fragment of 600–700 bp, and the C-terminal indole-3-glycerol-phosphate synthase function by a DNA fragment of about 900 bp, whereas both functions together are encoded by a contiguous DNA fragment of about 1,500 bp. The bi functional TRP3-peptide thus could be dissected into two catalytically independent peptides in vivo. For the indole-3-glycerol-phosphate synthase activity, independent catalytic activity was also demonstrated in vitro: deletions entering the TRP3 gene from the 5′-end, and lacking large parts of the sequence coding for the glutamine amidotransferase function, still are able to ex press a peptide exhibiting functional indole-3-glycerol phosphate synthase activity in vitro. Deletion plasmids pME505·De1C102·2μm and DelC10·2μm exhibited shorter TRP3 transcripts according to the deleted DNA-fragments (150 and 426 by respectively) but yielded peptides of invariable Mr of 35,000 d. Transcription and translation of these peptides, which probably represent the independently folding indole-3-glycerol-phosphate synthase core are discussed.
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  • 5
    ISSN: 1432-0983
    Keywords: Cephalosporium acremonium ; Mitochondrial DNA ; Autonomous replication sequence ; Saccharomyces cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A fragment of DNA which functions as an autonomous replication sequence in yeast was cloned from Cephalosporium acremonium. Mitochondrial DNA (mtDNA) was isolated from an industrial strain of C. acremonium (08G-250-21) highly developed for the production of the antibiotic, cephalosporin C. Size, 27 kb, and restriction pattern indicated this DNA was identical to mtDNA previously isolated (Minuth et al. 1982) from an ancestral strain (ATTC 14553) which produces very low amounts of cephalosporin C. A 1.9 kb Pst1 fragment of the Cephalosporium mtDNA was inserted into a Pst1 site of the yeast integrative plasmid, Ylp5, to produce a 7.5 kb plasmid, designated pPS1. The structure of pPS1 was verified by restriction analysis and hybridization. PS1 transformed Saccharomyces cerevisiae (DBY-746) to uracil prototrophy at a frequency of 272 transformants/μg DNA. Transformation frequencies of 715 transformants/μg DNA and zero were obtained for the replicative plasmid, YRp7, and the integrative plasmid YIp5, respectively. Southern hybridization and transformation of E. coli by DNA from yeast transformed by pPS1 verified that pPS1 replicates autonomously in yeast. The uracil-independent pPS1-yeast transformants were mitotically unstable. The average retention of pPS1 after three days growth in selective and non-selective medium was 4.5% and 0.4%, respectively, compared to retentions of 4.6% and 0.5% for YRp7. The properties of pPS1 were compared to those of a related plasmid, pCP2. pCP2 was constructed (Tudzynski et al. 1982) by inserting the C. acremonium 1.9 kb Pst1 fragment into the yeast integrative plasmid, pDAM1.
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  • 6
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; 5-aminolevulinate synthase ; Cloned gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have cloned the structural gene HEM1 for 5-aminolevulinate (ALA) synthase from Saccharomyces cerevisiae by transformation and complementation of a yeast hem1–5 mutant which was previously shown to lack ALA synthase activity (Urban-Grimal and Labbe Bois 1981) and had no immunodetectable ALA synthase protein when tested with yeast ALA synthase antiserum. The gene was selected from a recombinant cosmid pool which contained wild-type yeast genomic DNA fragments of an average size of 40 kb. The cloned gene was identified by the restauration.of growth on a non fermentable carbon source without addition of exogenous ALA. Sub cloning of partial Sau3A digests and functional analysis by transformation allowed us to isolate three independent plasmids, each carrying a 6 kb yeast DNA fragment inserted in either orientation into the single BamHI site of the vector pHCG3 and able to complement hem1–5 mutation. Analysis of the three plasmids by restriction endonucleases showed that HEM1 is contained within a 2.9 kb fragment. The three corresponding yeast trans formants present a 1, 2.5 and 16 fold increase in ALA synthase activity as compared to the wild-type strain. The gene product immunodetected in the transformant yeast cells has identical size as the wild-type yeast ALA synthase and its amount correlates well with the increase in ALA synthase activity.
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  • 7
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    Current genetics 9 (1984), S. 107-111 
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; 2 μm minichromosomes ; Metrizamide gradients
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two micrometer minichromosomes from Saccharomyces cerevisiae were isolated without detergent using metrizamide gradients. 2 μm minichromosomes showed a lower density in metrizamide gradients relative to genomic chromatin. Our results suggest a lower ratio of proteins to DNA in 2-μm minichromosomes as compared with genomic chromatin. The procedure described herein yields minichromosomes free of cellular chromatin and ribosomal protein contamination. This method may be useful for the isolation and characterization of other yeast minichromosomes.
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  • 8
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    Current genetics 8 (1984), S. 81-84 
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; Mitochondrial genes ; Vegetative segregation ; Uniparental inheritance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Zygotes of Saccharomyces cerevisiae that are heteroplasmic for mitochondrial alleles produce diploid progeny that are homoplasmic for one allele or the other, judged by the criterion that upon further subcloning they produce daughter cells of only one phenotype or the other. Here we show that when such cells are subjected to strong selection for the missing allele, it cannot be detected, so that it is probably not present in even a single copy.
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  • 9
    ISSN: 1432-0983
    Keywords: Iso-1-cytochrome c ; Saccharomyces cerevisiae ; Heme ; Transcription
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A Saccharomyces cerevisiae mutant (hem1 cycl-1) was transformed with plasmids bearing a chromosomal centromer (CEN3) and a 2 μm DNA replication origin. In one of the plasmids a functional CYC1 gene was present, in a second plasmid an XhoI fragment located between bases -245 and -678 upstream from the translation initiation codon had been deleted, in a third plasmid this region had been inverted. Results of hybridization experiments carried out with mRNA isolated from heme-deficient and heme-containing transformants indicated that heme controls transcription of the CYC1 gene and that DNA sequences located within the upstream XhoI fragment are involved in activation of the gene by heme.
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  • 10
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; TRP3 gene ; Sequence analysis ; Enzyme function
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The structure and function of the TRP3 gene of Saccharomyces cerevisiae were analyzed. Subcloning of an original 4.8 kb BamHI DNA fragment, carrying the yeast TRP3 gene, allowed for a localization of the gene on a 2.5 kb ClaI/BamHI fragment. Transcription was found to proceed from the ClaI site towards the BamHI site. Three major transcription start sites were determined at positions −92, −87, and −81 by S1-mapping. The synthesis of the TRP3 gene is regulated by the general control, and was found to take place- at the transcriptional level. The sequence of the 5′-noncoding region up to position −400 and part of the coding region to position 840 were determined. The 5′-noncoding region contains sequences common to most amino acid biosynthetic genes known so far, namely a presumptive ribosome binding site, “Goldberg-Hogness boxes”, and a consensus sequence, possibly involved in the general control. For the coding region a single open reading frame was found. The deduced amino acid sequence was aligned with homologous amino acid sequences of Neurospora crassa, Pseudomonas putida and Escherichia coli. The exceptionally high homology (40–60%) between these sequences led us to postulate that the TRP3 gene product is of the structure NH2-glutamine amidotransferase-indole-3-glycerol-phosphate synthase-COOH.
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  • 11
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; Cloning ; Suppressor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A plasmid, pYsup1-1, containing a DNA fragment able to suppress the recessive mutant phenotype of the suppressor locus sup1 (allele sup1-ts36) of Saccharomyces cerevisiae was isolated from a bank of yeast chromosomal DNA cloned in cosmid p3030. The complementing gene was localized on a 2.6 kb DNA fragment by further subcloning. Evidence is presented that the cloned DNA segment codes for the sup1 structural gene (chromosome IIR).
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  • 12
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    Current genetics 8 (1984), S. 575-580 
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; Candida utilis ; Protoplast fusion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Auxotrophic mutants of Saccharomyces cerevisiae and Candida utilis were hybridized through protoplast fusion. Spontaneous, UV- and FPA-induced mitotic segregation indicated that after cell fusion, exclusion of the S. cerevisiae nucleus or nuclear fusion followed by preferential loss of S. cerevisiae chromosomes can take place. Some of the hybrids were stable. One of them, expressed mating and sporulation functions of the S. cerevisiae parent. Thus, markers from both parents could be recovered as mitotic and meiotic segregants.
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  • 13
    ISSN: 1432-072X
    Keywords: Agglutination substance ; α Pheromone ; Cell cycle ; Ethyl N-phenylcarbamate ; Mating reaction ; Microtubules ; Saccharomyces cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Effects of ethyl N-phenylcarbamate (EPC) on the mating reaction of Saccharomyces cerevisiae were studied, with special attention on the effect on the α pheromone action. EPC inhibited zygote formation at a concentration which promoted induction of sexual agglutinability. EPC enhanced agglutinability induction by α pheromone, but inhibited α-pheromone-induced formation of large pearshaped cells in a mating type. The enhancement of agglutinability induction was accompanied with increased production of a agglutination substance and inhibition of α pheromone inactivation. EPC arrested the cell cycle of a cells probably in the step controlled by CDC19, CDC35, cAMP etc., just before the step controlled by CDC28, α pheromone etc.
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  • 14
    ISSN: 1432-072X
    Keywords: Saccharomyces cerevisiae ; Glucan synthetase ; EDTA ; Magnesium ; Sucrose ; Fluoride
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Yeast β(1–3) glucan synthetase is stimulated and stabilized by EDTA. Sucrose protects the enzyme from selfinactivaton. Preincubation of cell free extracts at low sucrose concentrations indicates a slow transition of the enzyme towards dissociation. Transition kinetics at 30° C and 0° C in the presence and in the absence of sucrose are interpreted assuming that a subunit is thermolabile in the free state and that sucrose increases its stability. Magnesium is deletereous for glucan synthetase in cell-free extracts. Chaotropic agents inactivate glucan synthetase according to their capacity to solubilize and depolymerize biological compounds. Fluoride plays a special role in the activation of glucan synthetase. Its action appears to be dependent on the presence of GTP (or other nucleotides). The role of all these agents on the activity and stability of the enzyme is interpreted in a unified scheme.
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  • 15
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    Archives of microbiology 137 (1984), S. 357-361 
    ISSN: 1432-072X
    Keywords: Yeast ; Saccharomyces cerevisiae ; Killer toxin ; Extracellular glycoprotein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A total of 13 killer toxin producing strains belonging to the genera Saccharomyces, Candida and Pichia were tested against each other and against a sensitive yeast strain. Based on the activity of the toxins 4 different toxins of Saccharomyces cerevisiae, 2 different toxins of Pichia and one toxin of Candida were recognized. The culture filtrate of Pichia and Candida showed a much smaller activity than the strains of Saccharomyces. Extracellular killer toxins of 3 types of Saccharomyces were concentrated and partially purified. The pH optimum and the isoelectric point were determined. The killer toxins of S. cerevisiae strain NCYC 738, strain 399 and strain 28 were glycoproteins and had a molecular weight of Mr=16,000. The amino acid composition of the toxin type K2 of S. cerevisiae strain 399 was determined and compared with the composition of two other toxins.
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  • 16
    ISSN: 1432-072X
    Keywords: Agglutination substance ; Cell-cell recognition ; Glycoprotein ; Mating ; Saccharomyces cerevisiae ; Sexual agglutinability ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An a-mating-type-specific substance responsible for sexual agglutination was purified to 397-times in specific activity (units/mg protein) from the cytoplasm of a-mating type cells. The purified substance gave a single band stained with PAS reagent but not with both Coomassie brilliant blue and silver staining reagent by polyacrylamide gel electrophoresis in the presence of 8 M urea. However, incorporation of [35S]methionine and Lowry reaction clearly indicate that the substance is a glycoprotein. The substance specifically masked sexual agglutinability of cells of the opposite mating type α, indicating univalent action. The substance is a glycoprotein with a carbohydrate content of 90%, a pI of 4.5, and a molecular weight of 130,000. The substance was inactivated by 2-mercaptoethanol and proteolytic enzymes but not by glycolytic enzymes. The substance formed a complementary complex having no biological activity when mixed with α-agglutination substance from the wall or cytoplasm of α-cells in vitro.
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  • 17
    ISSN: 1432-072X
    Keywords: Glycoprotein ; Inducible strains ; Saccharomyces cerevisiae ; Sexual agglutinability ; Tunicamycin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Effects of tunicamycin (TM) on the sexual agglutinability and zygote formation of Saccharomyces cerevisiae were studied using the two kinds of haploid strains, inducible and constitutive for sexual agglutinability. Induction of sexual agglutinability by opposite mating type sex pheromone of inducible strains was inhibited by TM in α mating type but not in a mating type. The recovery by temperature-shift-down from the temperature-suppressed sexual agglutinability of constitutive strains was enhanced by TM in a mating type but rather inhibited in α mating type. Pretreatment with TM of constitutive strains enhanced sexual agglutinability in a mating type but not in α mating type. The above-mentioned a-mating-type-specific agglutinability-enhancing actions of TM were discussed in relation to the action mechanism of α pheromone which induces or enhances the sexual agglutinability of a cells. Zygote formation was inhibited by TM in both constitutive and inducible strains at concentrations which showed only partially inhibitory effect on sexual agglutinability.
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  • 18
    ISSN: 1573-1561
    Keywords: Leptopilinaheterotoma ; Hymenoptera ; Eucoilidae ; Saccharomyces cerevisiae ; host-habitat searching ; chemoreception ; fermentation products ; ethanol ; ethyl acetate ; acetaldehyde
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Chemical stimuli play an important role in the process of searching for a host habitat by parasitic wasps. Volatile compounds originating from host habitats and/or hosts are the cues that enable such a location.Leptopilina heterotoma, a larval parasite ofDrosophila, is attracted to the food of its host, baker's yeast. Analysis of the fermentation products of baker's yeast, using a mass spectrometer, and olfactometer studies indicate that three fermentation products of this yeast, the main component of the host habitat in our laboratory, attractL. heterotoma: ethanol (5%), ethyl acetate (10−2, 10−3%), and acetaldehyde (1%). A combination of these three compounds, however, cannot compete with baker's yeast in attracting the parasites. Thus other factors, such as different compounds, concentrations, and/or combinations, also, play a role and remain to be tested.Leptopilina heterotoma does not use host-related olfactory cues in long-distance habitat location as it cannot distinguish between host habitat and host habitat with hosts.
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  • 19
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    Theoretical and applied genetics 68 (1984), S. 335-345 
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; 2D electrophoresis ; Developmental genetics ; Cytoplasmic inheritance ; Genetic regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two-dimensional gel electrophoresis of denaturated proteins were performed at five developmental stages or organs (hereafter referred to as stage-organs) on two wheat lines with four different cytoplasms. Five hundred and fifty to 712 reproducible spots were scored depending on the stage-organ. Each stage-organ is unambiguously characterized and several types of control of protein quantity are recorded. Post-translational modifications are hypothetized and may sometimes be stagespecific. Two cytoplasmic patterns are found: one for the euplasmic lines with Triticum aestivum cytoplasm and one for the alloplasmic lines with Aegilops juvenalis, Ae. ventricosa and Ae. kotschyi cytoplasms. Cytoplasmic variation is observed for 28 spots showing position difference, all of which are probably products of the LS gene, and for four spots showing differences for regulation of protein quantity. Nuclear variation between ‘Chinese Spring’ and ‘Selkirk’ is found for 20 allelic differences and for 20 regulatory systems, the latter number being probably underestimated.
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  • 20
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Gliadins ; Electrophoresis ; Hybridological analysis ; Genetic nomenclature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Inheritance of gliadin components in winter wheat has been studied by one-dimensional polyacrylamide gel electrophoresis. Single F2 grains from 36 intervarietal hybrid combinations have been analysed. The genetic analysis has revealed blocks, including 1–6 gliadin components, which are inherited as individual mendelian traits. About 80 variants of blocks have been detected. On the basis of the allelism test they are grouped into 6 series in accordance with the number of known gliadin-coding loci located on chromosomes of the homoeologous groups 1 and 6. Each series includes 8–18 blocks controlled by different alleles of one gliadin-coding locus. Blocks of components have been confirmed to be inherited codominantly in accordance to the gene dose in the triploid endosperm. The highest similarity between members of one series is observed in groups of blocks controlled by chromosomes ID and 6D. On the contrary, many blocks controlled by chromosomes 1A and 1B have no bands in common. The presented catalogue of blocks of components may be used to make up gliadin genetic formulae and to compare electrophoregrams obtained by different authors. Blocks of gliadin components are suitable genetic markers for use in revealing and studying heterogeneity of wheat varieties, in tracing their origin, in identifying recombinations, translocations and substitutions of the genetic material and in solving many other problems of the origin, evolution and selection of hexaploid wheat.
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  • 21
    ISSN: 1432-2242
    Keywords: Amphiplasty ; Ag-NOR ; Addition lines ; Nucleolar organizer ; Agropyron elongatum ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The nucleolar organizer activity of the Agropyron elongatum, its amphiploid with hexaploid wheat (Triticum aestivum) and the chromosome addition lines is analyzed by the silver-staining procedure. Four Ag-NORs are observed in A. elongatum corresponding to the chromosomes 6E and 7E. In the amphiploid T. aestivum — A. elongatum, eight Ag-NORs are observed which corresponds the wheat chromosomes 1B and 6B and to the elongatum chromosomes 6E and 7E. Thus, there is codominance in the nucleolar organizer activity of the chromosomes of the two species. However, a partial amphiplasty is detected since less than 8 Ag-NORs (7 up to 4) are observed in some metaphase cells; the chromosomes 6E and 7E are occasionally suppressed by wheat chromosomes. This conclusion is confirmed by the behaviour of the addition lines since only in those corresponding to the chromosomes 6E and 7E are the elongatum chromosomes nucleolar active although occasionally they can be suppressed by wheat chromosomes.
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  • 22
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    Theoretical and applied genetics 69 (1984), S. 193-198 
    ISSN: 1432-2242
    Keywords: C-banding ; Heterochromatin ; Meiosis ; Common wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The meiotic identification of nine pairs of chromosomes at metaphase I of meiosis of Triticum aestivum (B genome, 4A and 7A) has been achieved using a Giemsa C-banding technique. As a result, the analysis of the pairing of each chromosome arm in disomic and monosomic intervarietal hybrids between ‘Chinese Spring’ and the Spanish cultivar ‘Pané 247’ could be carried out. Differences in the chiasmata frequencies per chromosome arm cannot be explained on the basis of relative arm lengths only. Possible effects of arm-to-arm heterochromatic differences on meiotic pairing are discussed.
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  • 23
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Gliadins ; Two-dimensional electrophoresis ; Hybridological analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Blocks of gliadin components found both in a number of varieties and in single F2 grains of winter wheat intervarietal hybrids have been studied by two-dimensional electrophoresis combining electrophoresis in acidic aluminium-lactate buffer (pH3.1) and SDS-electrophoresis. Gliadin components (spots) have been shown to be inherited as linked groups (blocks), codominantly and in accordance with a gene dosage in triploid endosperm. Blocks include components differing in their electrophoretic mobility and molecular weight. Some allelic variants of blocks differ only in presence of few additional components or in the electrophoretic mobility of components with similar molecular weights; other variants may contain no similar components. Apparently, in the course of evolution, mutations in individual genes of gliadin-coding loci and processes changing the number of expressing genes and the sizes of their structural part occurred.
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  • 24
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    Plant systematics and evolution 145 (1984), S. 1-13 
    ISSN: 1615-6110
    Keywords: Angiosperms ; Poaceae ; Triticum aestivum ; Aegilops longissima ; Cytogenetics ; homoeologous relationships ; chromosome substitutions ; translocations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The relationships of three wheat-Aegilops longissima chromosome addition lines A, C, and D with homoeologous wheat chromosomes were studied in PMC meiosis. Substitutions of alien chromosome A for wheat chromosome 6 B, chromosome C for 1 B and chromosome D for 4 B were obtained. The production of 4 BS/C and 7 BS/D chromosome translocations indicated cytogenetic relationships of C partially to homoeologous wheat chromosomes of group 1 and 4, and D partially to homoeologous wheat chromosomes of group 4 and 7.
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  • 25
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; grain yield ; phosphate response ; height ; harvest index
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The response to phosphate fertilizer by modern wheat genotypes was examined in the field under natural rainfall in three seasons. Models were developed which show that grain yield was positively correlated with biological yield and harvest index. In one of the seasons, which was relatively dry, shorter statured wheats gave higher yields at each level of applied phosphate. Higher levels of phosphate tended to offset the reduction in yield associated with late heading and the importance of biological yield on grain yield. The genotypes which produced the largest number of grains m-2 produced the highest yields. Implications for plant breeding programs are discussed.
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  • 26
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    Euphytica 33 (1984), S. 107-112 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; male sterility ; meiotic analyses ; gene mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Genetic and cytogenetic studies were done on a male sterile mutant of the wheat variety ‘Probus’. Association of the 4A chromosome carrying the ms gene was studied in the F1 of the male sterile ‘Probus’ with ‘Chinese Spring’ ditelo 4AS, with ‘Transec’ and with line ‘T4AS-DRS’ respectively. The presumption that the genetic male sterility of the mutant was due to a terminal deletion of the short arm of chromosome 4A could be confirmed. Linkage studies showed that the ms gene was at 17 map units from the dwarfing gene (Rht3) of ‘Minister dwarf’. This allows selection of short male sterile plants at the seedling stage.
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  • 27
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; glutenin ; high molecular-weight subunits of glutenin ; SDS-PAGE ; 1R(1B) substitution identification ; 1BL/1RS translocation identification ; bread-making quality
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The high-molecular-weight subunits of glutenin from wheat 1R(1B) substitution and 1BL/1RS translocation lines were fractionated by SDS-PAGE. Two new subunits denoted R1 and R2 were characterized in 1R(1B) substitution, but not in 1BL/1RS translocation lines. R1 and R2 were proved to be rye proteins by 2d electrophoresis (NEPHGE x SDS-PAGE). In contrast to literature citations it was demonstrated that the cultivar Winnetou is a 1R(1B) substitution line and the cultivars Clement and Mildress both are 1BL/1RS translocation lines.
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  • 28
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; common wheat ; Puccinia striiformis ; yellow rust ; stripe rust ; resistance ; genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Three lines derived from the old ‘dirty’ Dutch land variety Gelderse Ris were resistant against race 66(70)EO(16) of yellow rust. It was found that this resistance was conditioned by one recessive gene provisionally coded yrGR.
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  • 29
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    Euphytica 33 (1984), S. 757-767 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; bread wheat ; Triticum durum ; durum wheat ; water stress ; photosynthesis ; stomatal resistance ; leaf water potential ; water efficiency ; breeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The responses of six wheat genotypes to water stress were analysed. Soil moisture (H), leaf water potential (Ψw), photosynthesis (PN), stomatal resistance (rs) and transpiration (T) were measured during a water stress. The genotypes investigated differed in their stress avoidance (Ψw-H relationship) and their stress tolerance (PN-Ψw and rs-Ψw relationships). The most important differences observed concern the mechanisms of tolerance at low leaf water potential: two varieties, Haurani 27 and Baalback, can then maintain a high photosynthetic activity. These observations are in agreement with the drought resistance characteristics already known for these genotypes. Possible applications to wheat breeding are considered
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  • 30
    ISSN: 1573-5060
    Keywords: Triticum dicoccoides ; wild emmer ; Triticum aestivum ; bread wheat ; nitrogen uptake ; plant nitrogen distribution ; translocation efficiency ; harvest index ; domestication ; evolution ; yield components ; grain protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Differences were found in total nitrogen uptake and its pattern of distribution in the main tiller amongst five lines of wild tetraploid wheat (Triticum turgidum dicoccoides) and between it and two hexaploid wheats (Triticum aestivum) under low (48 ppm) and higher (240 ppm) levels of soil nitrogen. Under the low soil nitrogen level the hexaploids had higher amounts of total nitrogen in the main tiller than the dicoccoides lines, but under the higher soil nitrogen level, three of the dicoccoides lines had significantly (P〈0.01) higher, and the other two lines, similar amounts as the hexaploids. The total amount of grain nitrogen in the hexaploids was significantly (P〈0.01) higher than the five dicoccoides under the low nitrogen soil level but under the higher level, two of the dicoccoides lines had similar amounts as one of the hexaploids (cv. Bencubbin) but significantly (P〈0.01) lower than the other (cv. Argentine IX). The efficiency of nitrogen translocation to the grain was significantly (P〈0.01) lower in a primitive, compared with four cereal forms of dicoccoides under both low and high levels of soil nitrogen. The cereal forms of dicoccoides, while similar in nitrogen translocation efficiency under low soil nitrogen as the lower translocation efficiency hexaploid (cv. Bencubbin), were significantly (P〈0.01) and substantially lower than it under the higher soil nitrogen level.
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  • 31
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    Plant and soil 78 (1984), S. 441-444 
    ISSN: 1573-5036
    Keywords: Gaeumannomyces graminis vartritici ; Hay-die ; Manganese deficiency ; Take-all ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The hypothesis that wheat plants deficient in managenese are predisposed to infection byGaeumannomyces graminis is outlined, and a test of the hypothesis in a soil system is reported. The results supported the hypothesis: wheat plants growing in managanese-deficient soil, although not showing foliar symptoms, were markedly more susceptible to infection; plant analysis confirmed the nutrient status of the plants. A review of the literature on take-all in wheat coupled with the results of our experiments suggests a reinterpretation of the etiology of this disease, since those edaphic factors which promote infection by this organism are those which also render managese unavailable to the host. Managenese nutrition is proposed as a common factor in many of the environmental conditions which influence the host-pathogen balance.
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  • 32
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    Euphytica 33 (1984), S. 91-98 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; near-isogenic lines ; chromosome substitution lines ; vernalization ; photoperiod, ear emergence ; basic development rate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Studies were made of days to ear emergence under the constant temperatures of 9, 14, 19 and 25°C and 16 h photoperiod in three sets of wheat lines each possessing genotypes differing for developmental responses. Days to ear emergence in three near-isogenic lines of the wheat cultivar Triple Dirk, which differed for vernalization response, increased as the strength of the response increased. At the four temperatures Triple Dirk D (Vrn 1 vrn 2) was not significantly different from normal Triple Dirk (Vrn 1 Vrn 2) but Triple Dirk B (vrn 1 Vrn 2) was significantly (P=0.01) later than normal Triple Dirk at each temperature. This indicates that the vrn 1 allele confers stronger vernalization response than vrn 2 over the range of temperatures (9–24°C). However, Triple Dirk C (vrn 1 vrn 2) failed to head after 120 days at each temperature indicating strong interaction between vrn 1 and vrn 2 with each other (and possibly the Triple Dirk back-ground) to give a much stronger vernalization response than predictions from additivity of their individual effects. The second set comprised the four Chinese Spring/Thatcher chromosome substitution lines CS/T 3B, 6B, 7B and 5D, plus Chinese Spring and Thatcher, and were grown in the unvernalized condition. CS/T 5D was similar in days to ear emergence as Chinese Spring at all four temperatures but the other three lines were earlier to ear emergence, particularly as the temperature increased. Days to ear emergence was fastest at 14°C in all lines, except CS/T 3B, in which it decreased progressively from 9 to 24°C. The third set of Chinese Spring and Thatcher and the homoeologous group 2 chromosomes of Thatcher substituted in Chinese Spring, the group which is considered to be involved in the control of photoperiod sensitivity. The three substitution lines responded differently to temperature compared with Chinese Spring and with each other, with chromosome 2D being the least, and chromosome 2B the most, responsive to temperature.
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  • 33
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    Euphytica 33 (1984), S. 123-132 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; durable resistance ; Puccinia striiformis ; yellow rust of wheat ; race-specific resistance ; race-non-specific resistance ; trangressive segregation ; stripe rust
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Crosses were made between wheat varieties Joss Cambier, Nord Desprez and Maris Bilbo, all classified as susceptible to yellow rust in field tests, and between Cappelle Desprez and Maris Huntsman, both classified as moderately and durably resistant. Selection for resistance to yellow rust among the progeny was carried out using races of Puccinia striiformis able to overcome all the known race-specific components of resistance in both parents of each cross. Lines with greater resistance than in both parents were obtained from each cross, those with greatest resistance being obtained from the cross between the moderately resistant parents. Three lines selected for resistance from the cross of Joss Cambier with Nord Desprez and one from the cross of Cappelle Desprez with Maris Huntsman, together with the parents, were tested in the field with 12 races of P. striiformis. Nord Desprez possessed a previously undetected race-specific component. The selected lines also displayed race-specific resistance, some of which was clearly related to race-specificity of the parents, and a component of resistance, greater than in both parents, that was effective against all 12 races. The possible origin and potential durability of this transgressive level of resistance is discussed. It is suggested that such transgressive resistance is more likely to be durable if it is derived from parents that have shown durable resistance.
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  • 34
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    Euphytica 33 (1984), S. 227-232 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; selection ; inbreeding ; maternal inheritance ; kernel color
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Data are presented which support early generation selection for white kernel color in the progeny of red/white kernel wheat crosses which are segregating at 12 or more loci for yield (or any other trait of interest). The optimum generation for selecting white kernels is determined by the frequency of seeds produced with the potential to produce plants having desired quantitative alleles from both parents, and by the frequency of white kernels produced. The F2-produced seed (F3 embryo) is shown to be the optimum generation for selecting white kernels, given that 12 or more loci are segregating for a quantitative character such as yield. When the red parent is a 2 or 3 loci red genotype, selection among F4-produced seed for white kernel color may be desirable when 5 or fewer genes are segregating for the second trait. The results have direct application for all highly heritable, recessive, sporophytic traits.
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  • 35
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    Euphytica 33 (1984), S. 233-239 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; read wheat ; Triticum durum ; durum wheat ; Secale cereale ; rye ; inbred lines ; intergeneric crossability ; embryo differentiation ; plantlet recovery
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary One hexaploid wheat cultivar (Triticum aestivem) and two tetraploid wheat lines (T. durum) were crossed with seventeen inbred lines of rye (Secale cereale). Seed set, degree of hybrid embryo differentiation at the time of excision for in vitro culture and recovery of amphihaploid plantlets from various embryo categories were studied. Degree of embryo differentiation was predominantly determined by maternal wheats, paternal rye genotypes appearing to be of minor importance. T. aestivum x rye hybrid embryos were superior to those produced from T. durum for degree of differentiation. The proportion of plantlets developing from differentiated embryos was high for all wheat parents, whereas undifferentiated embryos were mostly unsuitable for plantlet production. The results revealed that cross-incompatibility in hexaploid wheat x rye crosses was due to failure of fertilization, while in tetraploid wheat x rye crosses it was caused by lack of embryo differentiation. Correlation analyses showed that seed set provided a criterion to predict the amphihaploid plantlets to be expected from a particular wheat x rye combination.
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  • 36
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; bread wheat ; Triticum turgidum dicoccoides ; wild emmer ; homologous recombination ; homoeologous recombination ; Ph-mutants ; high protein ; kernel weight
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Wheat pentaploids were produced by hybridizing a high kernel weight (1000 grain wt=56 g), high protein (25.4%) line of wild tetraploid wheat (Triticum turgidum dicoccoides) as male parent, with the three hexaploids (T. aestivum) — normal Chinese Spring and its two homoeologous pairing mutants, ph 1b and ph 2. The pentaploids were crossed as female parents to the two commercial hexaploid cultivars Warigal and Barkaee and 42-chromosome stable plants selected from the F1 of the pentaploid x hexaploid crosses. Mean protein content of certain F3 lines from all six pentaploid x hexaploid crosses was significantly higher than Chinese Spring and the respective commercial hexaploid parent (p〈0.005) indicating high protein had been transferred from the tetraploid to the hexaploid level. Kernel weight amongst certain F3 lines of the three pentaploids x Barkaee was significantly (p〈0.0005) higher than either Chinese Spring or Barkaee, indicating the transfer also of high kernel weight from the tetraploid to the hexaploid level. However kernel weight was not significantly increased over Warigal in any F3 lines of its crosses with the three pentaploids. High levels of homoeologous chromosome pairing in the ph-mutant pentaploids, plus evidence for significant modification of the composition of high-molecular weight (HMW) glutenin subunits of grain protein in certain F3 derivatives of the ph-mutant pentaploid x hexaploid, crosses indicates that the ph-mutant-derived lines may possess novel (intergenome) genetic recombination, at least for high protein, and possibly kernel weight.
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  • 37
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    Euphytica 33 (1984), S. 489-495 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; multilines ; recurrent parents ; stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Three multilines each of Kalyansona and PV 18 varieties of wheat were compared for their stability of yield and agronomic characters and disease resistance against the respective recurrent parents. The experiments were conducted for four years at nine locations. The multilines had more tillers and bolder seeds than the recurrent parents. There were, however, no differences for plant height, ear length and number of spikelets per spike. The genotypes x years x locations interaction was much more important than genotypes x years or genotypes x locations interaction as well as the main effects for genotypes. It is suggested that the number of test locations should be increased while decreasing the number of years. The stability parameters indicated that the multilines had a higher mean yield in the case of Kalyansona multilines whereas the yield of multilines of PV 18 was not significantly inferior to that of the recurrent parent. The regression coefficients were very close to unity except for KSML 3 (b=1.132). The deviations from regression were much larger for the multilines than for the recurrent parents. For rust resistance the multilines were superior over the varieties Kalyansona and PV 18.
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  • 38
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; breeding ; grain yield ; grain quality ; preharvest sprouting
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Hard white winter wheat (HWWW) occupies a very limited area of the USA, but its purported advantages suggest that its production in the major hard red winter wheat (HRWW) region may be feasible. Objectives of our investigations were to develop experimental HWWW lines that combined desirable attributes-grain yield, functional grain quality, and resistance to preharvest sprouting-in single genotypes for comparison with popular cultivars in the major US RHWW region. Forty-four lines from seven parental combinations were tested in randomized complete block designs at three Kansas locations during the 1981–82 and 1982–83 seasons. Agronomic traits, grain yield, grain quality, and preharvest sprouting were measured. Plant characteristics and grain yield were similar in the HWWW experimental lines and the HRWW check cultivar, Newton. Mean grain SDS-sedimentation value and grain protein content of most experimental lines equaled or exceeded that of the check. Dough mixing times frequently were shorter for the experimental lines than for the check cultivar, whereas loaf volumes were greater. Falling number usually was similar in all geneotypes, but α-amylase was higher in field-harvested grain of white lines than the check; both measures were more favorable than grain trade standards. We concluded that production of high yielding, high quality hard white winter wheat genotypes is feasible in the US ‘breakbasket’.
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  • 39
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    Plant and soil 80 (1984), S. 261-266 
    ISSN: 1573-5036
    Keywords: Azospirillum brasillense ; Wheat yield ; Triticum aestivum ; Responding genotypes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Twenty different wheat genotypes representing a wide genetic variability, were tested for their yield and yield components response to inoculation withAzospirillum brasilense, at two levels of N fertilization. Only two cultivars responded by a significant yield increase of 7.4 and 8.0 per cent — both at the higher N level. The response reflected an increase in the number of grains per plant added as additional spikes. The importance of the host plant genotype for a successful wheat-bacteria association is discussed.
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  • 40
    ISSN: 1573-5036
    Keywords: Lignin ; Manganese ; NO 3 − Phenols ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Managanese deficiency (〈 18 μg g−1 Mn) resulted in decreased levels of phenols in wheat shoots and decreased levels of lignins in both roots and shoots. These observed reductions in phenol contents was due largely to a decrease in the alkaline labile phenol component. Levels of nitrate supplied in solution influenced both phenol and lignin production; high nitrate levels (15 mM) resulted in a reduction in phenol and lignin in the shoot but stimulated lignin production in root tissue.
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  • 41
    ISSN: 1573-5036
    Keywords: Azospirillum lipoferum ; Grain yield ; N-content ; Nitrogen fixation ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Spring wheat (Triticum aestivum var. Arkas) was associated withAzospirillum lipoferum under greenhouse and field conditions of a temperate region. Controls were treated with autoclaved bacteria. The soils used were: sand, sandy loam, and a peat-clay mixture. In experiments run over a period of three years, there were increases in grain yield, N-yield of the grains, and 1000 grain weight. Depending from environmental conditions, increase changed from year to year, and within one given year. There was, however, no experiment without positive response to the inoculation. Highest grain yield increase (70%) was found on sand supplemented with P and K only, but up to 32% were also obtained on peat-clay soil containing 0.28% total N. Under greenhouse conditions, one third of technical N-fertilizer could be saved by bacterial activities. With high probability the effects observed have been at least partly due to bacterial N2-fixation, because the N-yield of the grains was increased (up to 33%), and the most pronounced response was found on sand without any N-fertilizer added.
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  • 42
    ISSN: 1573-6881
    Keywords: H+-ATPase complex ; assembly defect ; Saccharomyces cerevisiae ; mitochondrial biogenesis ; membrane association
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Physics
    Notes: Abstract We have investigated the extent to which the assembly of the cytoplasmically synthesized subunits of the H+-ATPase can proceed in a mtDNA-less (rho°) strain of yeast, which is not capable of mitochondrial protein synthesis. Three of the membrane sector proteins of the yeast H+-ATPase are synthesized in the mitochondria, and it is important to determine whether the presence of these subunits is essential for the assembly of the imported subunits to the inner mitochondrial membrane. A monoclonal antibody against the cytoplasmically synthesized β-subunit of the H+-ATPase was used to immunoprecipitate the assembled subunits of the enzyme complex. Our results indicate that the imported subunits of the H+-ATPase can be assembled in this mutant, into a defective complex which could be shown to be associated with the mitochondrial membrane by the analysis of the Arrhenius kinetics of the mutant mitochondrial ATPase activity.
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  • 43
    ISSN: 1573-5044
    Keywords: Triticum aestivum ; wide crosses ; tissue culture ; somaclonal variation ; Agropyron
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Segments of young inflorescences of Triticum aestivum cv. Chinese Spring (CS), its F1 hybrids with Agropyron trachycaulum and A. scirpeum and backcross derivatives with A. yezoense, A. intermedium and A. junceum, and of a A. yezoense x T. aestivum cv. Wichita hybrid were cultured. Different parts of young spikelets of A. trachycaulum x CS F1 and A. yezoense x Wichita F1 's were also cultured. Percent callus induction was lower in wheat than in the wheat-Agropyron hybrids or backcross derivatives. Percent callus induction from different organs in both hybrids was in the descending order of whole spikelet, spikelet without glumes, rachis, and glumes. No plants could be regenerated from calli of wheat and backcross derivatives except those of CS x A. intermedium combination. Callus induction in hybrids varied from 54 to 84% and plant regeneration from 14 to 31%. The regenerants required no vernalization. Variants including one with top-dense spikes and another with elongated spikelets were recovered. Out of eight A. trachycaulm x CS hybrid regenerants, one had anthers and stigma as opposed to neutral flowers of the original hybrid.
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  • 44
    ISSN: 1573-5036
    Keywords: Azospirillum brasilense ; Wheat ; Triticum aestivum ; N-fertilizer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The yield response of a common spring wheat cultivar,Triticum aestivum, to inoculation withAzospirillum brasilense was studied at four levels of N fertilization. Plant yield increased due to the inoculation treatment only at medium and high levels of N fertilization, with a maximum yield increase of about 8.0 per cent at the highest level (approximately 1.0 g of pure N per plant). Yield increase was mostly due to an increase in the number of grains per spike, and at the highest level of fertilization, also due to a higher number of spikes per plant. At all N levels, the inoculation caused an increase of 0.5–1.4 per cent in the number of fertile spikelets per main spike. Grain protein percentage was unaffected by the inoculation, though significantly increase due to the fertilization treatments. The occurrence of maximum yield response at the highest N level, the response by early-determined yield components, i.e. spikelet number, and the unaffected grain protein content are in accord with the suggestion that the contribution ofAzospirillum brasilense to wheat yield is not through N2-fixation.
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  • 45
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    In:  Berlin, Springer, vol. 6, no. 1, pp. 1-40, (ISBN: 0-444-51340-X)
    Publication Date: 1984
    Keywords: Textbook of mathematics ; lineare ; Algebra ; Inversion ; Eigen-value ; MINV ; Zurmuhl
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  • 46
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    European journal of nutrition 22 (1983), S. 205-212 
    ISSN: 1436-6215
    Keywords: Schwermetallwirkung ; Malatdehydrogenase ; Glutamatdehydrogenase ; Glycerinaldehyd-3-phosphatdehydrogenase ; Saccharomyces cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Medicine
    Description / Table of Contents: Summary The difference between cadmium, zinc, lead, and mercury in regard of their effects on the activity of the enzymes tested is very slight. Concentrations higher than 10−5 M reduce significantly the activity of the enzymes, and concentrations of approximately 10−3 M inhibit it completely. An increase of the activity cannot be detected. The addition of combinations of cadmium, zinc, and lead results in a summing up of the toxic effects, whereas the interaction between mercury and the other three heavy metals shows a cumulative effect, which is appointed nearly completely by the heavy metal more toxic. The findings suggest that under in-vitro conditions there exists a direct interaction between the heavy metals and the enzymes.
    Notes: Zusammenfassung Die vier Schwermetalle Cadmium, Zink, Blei und Quecksilber unterscheiden sich in ihrer Wirkung auf die Aktivität der untersuchten Enzyme nur sehr wenig. Konzentrationen über 10−5 M vermindern die Enzymaktivität signifikant, und Konzentrationen von etwa 10−3 M unterbinden sie völlig. Eine Steigerung der Enzymaktivität läßt sich nicht feststellen. Die Zugabe von Cadmium-, Zink- und Bleikombinationen führt zu einer Addition der toxischen Effekte, während bei der Interaktion zwischen Quecksilber und den anderen drei Schwermetallen die Gesamtwirkung fast ausschließlich durch das stärker hemmende Schwermetall allein bestimmt wird. Die erhaltenen Ergebnisse lassen vermuten, daß es unter Invitro-Bedingungen zu einer direkten Wechselwirkung zwischen den Schwermetallen und den Enzymen kommt.
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  • 47
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    Current genetics 7 (1983), S. 165-166 
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; Cephalosporium acremonium ; Mitochondrial hybrid vector ; Nuclear association
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The hybrid vector pCP2, consisting of the bacterial plasmid pBR325, the nuclear gene Leu-2 of Saccharomyces cerevisiae and a fragment of mitochondrial DNA from Cephalosporium acremonium, was found to associate with the nucleus in a transformed strain of Saccharomyces cerevisiae. This was inducted by (1) efficient expression of the Leu-2 gene as evidenced by a short generation time on selective medium; (2) independence of Leu-2 gene expression from mitochondrial protein synthesis, since pCP2 was shown to replicate and to be expressed in petite mutants; (3) association of pCP2 with isolated DNA from nuclei as proved by transformation experiments with E. coli.
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  • 48
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; G1 cdc mutants ; tα-factor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mutants in four G1 cdc strains of Saccharomyces cerevisiae were isolated which failed to show division arrest in the presence of α-factor. The cell cycle properties, terminal arrest morphology and mating competence of these mutants at the restrictive temperature were examined. The G1 specific arrest of the cdc 36 and cdc39 mutants is dependent upon the availability of an intact mating factor response system in Mat a cells. Cdc28 and cdc37 mutants exert their cell cycle blocks independently of the mating factor pathway. It is likely that the nature of the primary growth defect in cdc36 and cdc39 mutants is such that the α-factor pathway is activated in the absence of the pheromone at the restrictive temperature and that G1 arrest is a secondary consequence of a non-cycle specific event in such mutants.
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  • 49
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    Current genetics 7 (1983), S. 235-237 
    ISSN: 1432-0983
    Keywords: DNA replication ; Shuttle vectors ; Saccharomyces cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mitotic segregation of three 2 μm-pBR322 chimaeric plasmids (YEp6, YEp21, and YEp24) was studied in yeast. Each displayed a characteristic rate of loss: YEp6 was lost at approximately twice the rate of YEp21 and YEp24. The loss rates were not significantly increased when two chimaeric plasmids were coresident, nor was the endogenous 2 μm plasmid itself displaced. Therefore these plasmids appear to be compatible in yeast.
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  • 50
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; DEL1 ; rad ; ste7
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In DEL1 strains of the yeast, Saccharomyces cerevisiae, the iso-1-cytochrome c (CYC1) region is flanked on either side by Tyl elements in direct orientation which promote cyc1 deletions of the bracketed DNA in the haploid cell. In this study, we asked which genes might control this event by testing the possibility that the DEL1 mutation mechanism requires an enzyme (or enzymes) that is also utilized in the repair of damaged DNA. To this end, we independently coupled eight repair mutations, rad3–2, rad4–4, rad6–1, rad6–3, rad9–1, rev3–1, rad50–1, and rad51-1, toDEL1 and asked whether DEL1 was still functional. We found that none of these rad mutations significantly affects the mutation frequency of 10−6-10−5 established in DEL1 strains for the CYC1 locus. Furthermore, we determined that ste7, a temperature-sensitive sterile allele known to alter gene regulation in Ty-mediated mutations, is not required for DEL1 function. Finally, DEL1 is not temperature-sensitive at 23° or 37 °C.
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  • 51
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    Current genetics 7 (1983), S. 369-377 
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; Isoenzymes ; Induction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Tetrad analysis indicates that α-isopropylmalate synthase activity of yeast is determined by two separate genes, designated LEU4 and LEU5. LEU4 is identified as a structural gene. LEU5 either encodes another α-isopropylmalate synthase activity by itself or provides some function needed for the expression of a second structural gene. The properties of mutants affecting the biosynthesis of leucine and its regulation suggest that the expression of LEU1 and LEU2 (structural genes encoding isopropylmalate isomerase and β-isopropylmalate dehydrogenase, respectively) is controlled by a complex of a-isopropylmalate and a regulatory element (the LEU3 gene product). Similarities and differences between yeast and Neurospora crassa with respect to leucine biosynthesis are discussed.
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  • 52
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    Current genetics 7 (1983), S. 393-397 
    ISSN: 1432-0983
    Keywords: Trehalose ; Glycogen ; Sporulation ; Germination ; Saccharomyces cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mutants with specific lesions were used to differentiate between the functions of glycogen and trehalose in S. cerevisiae. Diploids which harbor the glc1/glc1 mutation depend upon the phosphorylated, less active form of glycogen synthase and show a more active, phosphorylated form, of the enzyme trehalase. These conditions are due to a lesion in the regulating subunit of the cAMP-dependent protein kinase. Such cells are unable to sporulate. Diploids which contain the sst1/sst1 mutation have normal glycogen metabolism but their trehalose-6-phosphate synthase is not active. Such strains sporulate but germination is poor and only one-spore tetrads are formed. These results confirm that glycogen is needed to trigger sporulation while trehalose plays a role in the germination process. Different systems, I and II, of trehalose accumulation were proposed. System I would require the UDPG-linked trehalose synthase, whereas system II would constitute an alternative pathway, specifically induced or activated by the expression of a MAL gene. The presence of system II in its constitutive form in the constructed diploids would favour trehalose synthesis during growth on glucose, however, it did not overcome the glycogen deficiency during sporulation nor the lack of trehalose for germination. It seems that only system I, namely trehalose 6-P-synthase, plays a role in the germination process.
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  • 53
    ISSN: 1432-0983
    Keywords: Oversecretion mutants ; Protease defect ; Wall glucan defect ; Saccharomyces cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two chromosomal mutations in yeast that result in oversecretion of the K1 killer toxin protein were examined. A recessive mutation in gene ski5 appears to lead to toxin oversecretion through a defect in a cell surface, PMSF-inhibited protease. A wild type killer strain degraded toxin following synthesis, and degradation could be partially prevented by addition of PMSF to the growth medium. The ski5 mutation caused an approximate ten fold oversecretion of toxin, similar to that seen in a PMSF-treated wild type culture, and no increased oversecretion in the presence of PMSF. The ski5 mutation caused oversecretion of other low molecular weight secreted proteins and appeared to oversecrete the α-factor pheromone, as judged by activity tests. The ski5 mutation was complemented by mutations in ski genes 1–4, and the mutant was not supersensitive to mating pheromones or K2 killer toxin. We also examined killer strains with a mutation in the nuclear gene krel which results in a defective (1→6)-β-D-glucan cell wall receptor for killer toxin. Such strains oversecrete toxin into the growth medium, but also, unexpectedly, oversecrete most other secreted proteins. The defect in (1→6)-β-D-glucan in these mutants appears to perturb the partitioning of secreted proteins between the cell wall and the medium.
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  • 54
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    Current genetics 7 (1983), S. 427-431 
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; DNA ; Alkaline elution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The method of analysis of DNA in mammalian cells by alkaline elution from filters (Kohn et al. 1974) was adapted for studies on yeast DNA. By this technique spheroplasts obtained from yeast cells are lysed on filters and single-stranded DNA fragments selectively eluted by alkaline solutions. The procedure was applied to monitor the occurrence of replication intermediates and production of DNA single-strand breakage by MMS, and its repair in growth medium.
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  • 55
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    Current genetics 7 (1983), S. 433-438 
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; Yeast transformation ; Yeast autonomously replicating sequences ; Ribosomal RNA genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have previously demonstrated that the loss of Rcp-CEN3, a centromeric plasmid containing yeast rDNA autonomously replicating sequences (ARS) is as high as around 50% per generation for most yeast strains. In this study we have attempted to elucidate mechanisms underlying the high mitotic instability of Rcp-CEN3. For this purpose a tandem duplication of a rDNA ARS was constructed in Rcp-CEN3. The new plasmid having two ARSs possesses a markedly higher mitotic stability as compared to a monoARS Rcp-CEN3. The mitotic stability of this centromere-containing plasmid which has two replicators corresponds to the calculated value for the mitotic stability of two monoARS plasmids Rcp-CEN3 in given cells. Genetic analysis has demonstrated that both plasmids having one or two ARSs are maintained in the single copy state. These results demonstrate that the mitotic instability of centromeric plasmid Rcp-CEN3 carrying a rDNA ARS is associated with the absence of stringent control of replication from the rDNA ARS. A possible mechanism of replication of the chromosomal rDNA repeats in yeast is discussed in the light of this data.
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  • 56
    ISSN: 1432-072X
    Keywords: α Pheromone ; Cell cycle ; G1 arrest ; Hansenula wingei ; Saccharomyces cerevisiae ; Shmoo
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The cell cycle of a (5) mating type cells of Hansenula wingei was arrested in the G1 phase by α pheromone of Saccharomyces cerevisiae but not by α(21) pheromone of H. wingei, although both the α pheromones are known to induce sexual agglutination ability of a mating type cells of H. wingei. Cells of α mating type of H. wingei became shmooed or arrested in the G1 phase in response to neither a pheromone of H. wingei nor α pheromone of S. cerevisiae.
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  • 57
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    Archives of microbiology 134 (1983), S. 64-67 
    ISSN: 1432-072X
    Keywords: Saccharomyces cerevisiae ; Anaerobic growth ; Hungate technique ; Tween 80 ; Ergosterol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Saccharomyces cerevisiae, Nontrachet strain 522 was successfully grown anaerobically on various glucose concentrations in Yeast Nitrogen Base (YNB) medium (pH 3.5) prepared under an atmosphere of carbon dioxide (CO2). This growth occurred in the absence of Tween 80 and ergosterol. The medium, prepared using the Hungate technique for cultivation of strictly anaerobic bacteria, contained the reducing agent cysteine·HCl·H2O (0.03%). Anaerobic growth was stimulated by the addition of Tween 80 and ergosterol to the anaerobic medium.
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  • 58
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    Archives of microbiology 134 (1983), S. 171-174 
    ISSN: 1432-072X
    Keywords: Acetate growth medium ; Anti-microtubule agent ; Bud initiation ; Ethyl N-phenylcarbamate ; Meiosis ; Mitotic cell cycle ; Saccharomyces cerevisiae ; Sporulation induction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract When diploid cells of Saccharomyces cerevisiae were incubated in acetate growth media containing 2.5 mM ethyl N-phenylcarbamate (EPC), bud initiation was inhibited preferentially, and eventually overgrown, unbudded cells accumulated. During subsequent incubation, meiosis and ascospore formation occurred at high frequencies. The behavior of EPC-treated cells was essentially the same as that of cells transferred to a starvation sporulation medium. EPC thus has a pronounced effect on the mitotic growth of yeast cells, which leads to meiotic development. Our observations indicate that EPC has a decisive function in the initiation of meiosis in rich growth media.
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  • 59
    ISSN: 1432-072X
    Keywords: Nucleotide pools ; Continuous cultivation ; Synchronized growth ; Saccharomyces cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract High pressure liquidd chromatography has been used to study the acid soluble nucleotide pool of Saccharomyces cerevisiae under different conditions of growth. ATP, ADP, AMP, NAD, GTP, UTP, UDP, CTP, CDP, and UDP-sugars plus UMP could be separated and were found in concentrations higher than 0.1 μmol per g yeast cell dry weight (=detection limit). During glucose-limited continuous culture the levels of individual nucleotides depended on the growth rate, which was most pronounced with pyrimidine (uridine, cytidine) nucleotides. The energy charge (E.C.) remained high (0.9) at all growth rates (0.07–0.3 h-1). During synchronized growth at a constant growth rate (0.11 h-1) almost all nucleotide levels and the E.C. remained at constant values with the only exception of UDP-sugars and UMP of which increased levels were found during the phase of budding. Under conditions of metabolic stress (addition of antimycin A, deoxyglucose plus iodoacetate) pronounced changes in the levels of purine (adenine and guanine) nucleotides and the E.C. were observed. All other nucleotides were less influenced by these conditions. Only under these conditions IMP accumulation was observed. The results strongly argue against the significance of purine nucleotide or E.C. measurements under viable conditions. In contrast, changes in the levels of pyrimidine nucleotides seem to be indicative of changes in the flux through the metabolic pathways where they act as coenzymes.
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  • 60
    ISSN: 1432-1424
    Keywords: electron probe X-ray microanalysis ; Saccharomyces cerevisiae ; ethidium ; brontophenol blue ; cationic dye ; cytolysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Summary K+ efflux provoked by ethidium proceeds partially as an all-or-none effect by which the diffusion barrier for K+ is disrupted and partially from still intact cells, presumably by exchange against ethidium. This is shown by the application of an electron probe microanalysis X-ray technique by which the K+ content of a number of individual cells is analyzed.
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    Theoretical and applied genetics 66 (1983), S. 101-109 
    ISSN: 1432-2242
    Keywords: Anther culture ; Culture temperature ; Induction frequency ; Pollen callus (plantlet) ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The response of anther culture to culture temperature was studied in detail using many varieties, F1 hybrids and pollen-derived lines of wheat (Triticum aestivum) as materials. The suitable culture temperature for inducing pollen callus (or embryoids) in wheat anther culture ranged from 26 °C to 30 °C, varying with genotypes. But for the great majority of wheat genotypes the suitable culture temperatures lay between 28 °C and 30°C. The most significant genotypic variation in the response to culture temperature was observed in the comparison between the culture at 33 °C for eight days followed by culture at 25 °C (or 26 °C) and the continuous culture at 25 °C (or 26 °C). This genotypic variation in the response to culture temperature is a heritable character which may be controlled by multiple genes. The effect of culture at 30 °C for eight days followed by culture at 26 °C was similar to, or in some cases, better than that of continuous culture at 28 °C, and the effect of culture at 32 °C for eight days followed by culture at 28 °C was similar to that of continuous culture at 30 °C. In the range from 26 °C to 32 °C, the overwhelming majority of pollen calli emerged before the 40th day after anther inoculation, and the higher the culture temperature, the earlier and more concentrated the emerging period of the pollen callus. The pollen callus obtained at high temperatures above 28 °C should be transferred in time onto the regeneration medium at 25°–27°C to induce shoots.
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  • 62
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    Theoretical and applied genetics 64 (1983), S. 255-258 
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Wheat ; Malate dehydrogenase ; Chromosomal location
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The malate dehydrogenase (E.C. no 1.1.1. 37) of Triticum aestivum L. cv. Chinese Spring, shows two activity zones. The results obtained support the hypothesis that the malate dehydrogenase isozymes of zone II are dimers composed of the six possible combinations of subunits coded by triplicate genes located in the long arms of chromosomes of the homoeologous group 1.
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  • 63
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    Theoretical and applied genetics 66 (1983), S. 111-121 
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Agropyron ; Intergeneric hybrids ; Embryo culture ; Chromosome pairing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Intergeneric hybrids of Triticum aestivum (2n=42,AABBDD) with Agropyron ciliare (2n= 28,SSYY), A. trachycaulum (2n=28,SSHH), A. yezoense (2n=28,SSYY) and A. scirpeum (2n=28) are reported for the first time. F1 hybrids of T. aestivum were also produced with A. intermedium (2n=42,E1E1E2E2Z1Z1) and A. junceum (2n=14,JuJu). All wheat-Agropyron hybrids were obtained by embryo rescue technique. Cultivars and reciprocal crosses differed for seed set, seed development and F1 plant production. The F1 hybrids were sterile. Attempts to obtain amphiploids were unsuccessful. However, backcross derivatives were obtained with wheat as the recurrent parent. The level of chromosome pairing in A. trachycaulum x wheat, A. yezoense x wheat and wheat x A. junceum hybrids provided no evidence of homologous or homoeologous pairing. Mean pairing frequencies in A. ciliare x wheat, wheat x A. scirpeum and wheat x A. intermedium hybrids indicated homoeologous or autosyndetic pairing. Ph gene was more effective in regulating homoeologous pairing in A. yezoense x wheat hybrids than in A. ciliare x wheat hybrid. Chromosome pairing data of BC1 derivatives indicated that either some of the wheat chromosomes were eliminated or Agropyron chromosomes caused reduced pairing of wheat homologues.
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  • 64
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    Theoretical and applied genetics 65 (1983), S. 41-46 
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Wheat ; Protein ; Mutation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Poor adaptability or functional quality of much germplasm used for breeding high-protein hard red winter wheats prompted mutagenesis as an alternative means of increasing grain protein content. Four hard red winter wheat genotypes — KS644 (‘Triumph// Concho/Triumph’), ‘Kaw’, ‘Parker’, and ‘Shawnee’ — were treated with 0.40 M ethyl methanesulfonate (EMS). Advanced lines (M8-M10) were selected that had a 3-year mean grain protein advantage of 0.7% to 2.0% over controls. Increased grain protein content was generally associated with decreased grain yield and kernel weight, but some high-protein mutant lines had yields or kernel weights similar to those of original genotypes. Changes in height and lodging induced by EMS were generally favorable, most mutants being shorter and lodging less than controls, but blooming date was generally delayed, a deleterious change. One line also changed from resistant to segregating for wheat soil-borne mosaic virus. Mutant lines might be utilized in cross-breeding programs, particularly if negative pleiotropic effects and linkages are absent.
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  • 65
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    Theoretical and applied genetics 64 (1983), S. 97-101 
    ISSN: 1432-2242
    Keywords: Common wheat ; Triticum aestivum ; Electrophoresis ; Endosperm proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Total endosperm protein subunits, extracted from the common wheat cultivar Chinese Spring and from some of its aneuploid lines, were fractionated according to their molecular weight (MW) in an improved high resolution one-dimensional sodium dodecyl sulphate (SDS) polyacrylamide gel electrophoresis (PAGE). The resolution obtained by this method and, in particular, that of the high molecular weight (HMW) glutenin and gliadin subunits approached that of a previous report in which two-dimensional fractionation system based on charge and MW was used. In the cultivar Chinese Spring, 21 discrete protein bands were resolved and the chromosomes controlling many of them were either reconfirmed, or, in some cases, established. The advantages of this high resolution SDS PAGE technique are discussed.
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  • 66
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    Theoretical and applied genetics 67 (1983), S. 53-58 
    ISSN: 1432-2242
    Keywords: Aegilops umbellulata ; Genetics ; Lectin ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Each of the three genomes in hexaploid wheat controls the expression of a specific lectin in the embryo. The chromosomes which control their synthesis were determined using nullisomic-tetrasomic and inter-varietal chromosome substitution lines of ‘Chinese Spring’. All three wheat lectins were shown to be controlled by the homoeologous group 1 chromosomes. Using ditelosomic lines of ‘Chinese Spring’ the lectin genes could be localized on the long arms of chromosomes 1A and 1D. Inter-specific addition and substitution lines of Aegilops umbellulata chromosomes to ‘Chinese Spring’ indicated that chromosome 1U, which is homoeologous to the group 1 chromosomes of wheat, controls lectin synthesis.
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  • 67
    ISSN: 1432-2242
    Keywords: HMW glutenin subunit genes ; cDNA clones ; Tandem DNA repeats ; Chromosomal location ; Gene copy number ; Wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary cDNA clones encoding wheat HMW glutenin subunits have been isolated from a cDNA bank made to poly A+ RNA from developing wheat endosperm var. Chinese Spring. One such clone, pTag 1290, has enabled us to identify the HMW glutenin mRNA species. The DNA sequence of this clone has been partially determined and it contains several tandem DNA repeats. The sequence is discussed in relation to the generation of the HMW glutenin subunit gene family. Analysis of the organization of the HMW glutenin sequences in the wheat genome revealed that the genes encoding HMW glutenin subunits exist in low copy number and are located on the long arm of each of the homoeologous group 1 chromosomes.
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  • 68
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; T. timopheevii ; Wheat ; Photoperiod ; Vernalization ; Male sterility ; Alloplasmic hybrids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Studies were conducted to determine the influence of the male sterility-inducing cytoplasm of Triticum timopheevii (Zhuk.) Zhuk. on response of several common winter wheat (T. aestivum L.) nuclear genotypes to photoperiod and vernalization. Comparative studies of cytoplasmic substitution lines provide information on the role of the cytoplasmic genetic mechanism in growth and development. In the case of cytoplasmic male sterility-based hybrid production systems, ubiquity of sterility-inducing cytoplasm in derived hybrids warrants thorough characterization of its influence on plant phenotype. Factorial combinations of cytoplasm (T. timopheevii and T. aestivum), nuclear genotype, and photoperiod or vernalization treatments were evaluated under hydroponic conditions in controlled environment chambers. Interaction of cytoplasm, photoperiod, and nuclear genotype was significant in one or more experiments for days to anthesis and potential spikelet number, and interaction of cytoplasm, vernalization, and nuclear genotype was significant for days to spike emergence. Long day length was associated with increased percentage seed set in one study, but interactions of photoperiod and cytoplasm were not detected for percentage seed set. Interactions involving cytoplasm and photoperiod or vernalization were interpreted as evidence of the existence of genetic factors in cytoplsam of T. timopheevii which alter photoperiod or vernalization responses of alloplasmic plants relative to responses exhibited by euplasmic plants. Since photoperiod and vernalization responses are critical to adaptation, T. timopheevii cytoplasm can alter adaptability of T. aestivum. The specific effect would be nuclear genotype dependent, and does not appear to be of a magnitude greater than that induced by nuclear genetic variability at loci conditioning photoperiod or vernalization responses or other adaptation-determining characteristics. Normal multilocation/year testing of alloplasmic hybrids should therefore adequately identify zones of adaptation.
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  • 69
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    Theoretical and applied genetics 66 (1983), S. 77-86 
    ISSN: 1432-2242
    Keywords: Common wheat ; Triticum aestivum ; Electrophoresis ; Endosperm proteins ; Glutenins ; Gliadins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Endosperm protein subunits of 109 primitive and modern lines of hexaploid wheat, Triticum aestivum L. em. Thell., were fractionated by one-dimensional, high resolution, sodium dodecyl sulphate (SDS) polyacrylamide gel electrophoresis (PAGE). A wide range of both qualitative and quantitative variation was observed in the fractions of the high molecular weight (HMW) glutenin and gliadin subunits of the different lines. The qualitative variation was expressed in the number of subunits per fraction and in their molecular weight, as determined by the differential rate of migration. The quantitative variation was expressed in the differential staining intensity of several subunits. The widest variation was detected in the HMW glutenin and gliadin subunits controlled by chromosome 1B while a much smaller variation was observed in those subunits controlled by chromosome 1A and further smaller variation in the subunits controlled by 1D. Only a small number of subunits in both fractions was found to be controlled by chromosome 1A indicating that diploidization of endosperm protein genes in common wheat has been non-random. The genetic and evolutionary implications of these findings are discussed.
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    Theoretical and applied genetics 66 (1983), S. 1-7 
    ISSN: 1432-2242
    Keywords: Triticum aestivum ; Aegilops species ; Alloplasmic lines ; 2D gel electrophoresis ; Cytoplasmic inheritance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In this first analysis the protein patterns obtained by two-dimensional gel electrophoresis of 8 day-old leaves from 18 alloplasmic wheat lines are compared. From 440 spots retained on the basis of their reproducibility, 36 proteins were observed to vary in different cytoplasms, allowing us to distinguish the T. aestivum cytoplasm from 5 Aegilops cytoplasms. Twenty-four of the 36 variable proteins could be structurally related to the large subunit of RuBPCase. Nuclear variation between 3 wheat varieties was observed for 14 proteins.
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  • 71
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    Protoplasma 115 (1983), S. 104-113 
    ISSN: 1615-6102
    Keywords: Cereals ; Embryo culture ; Triticum aestivum ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Cell proliferation from the mature embryo of wheat occurs on a defined medium in the presence of 2,4-D. Unorganized growth is observed when the 2,4-D concentration is equal to or greater than 2 mg/l, but increasing levels of 2,4-D inhibit cell division. Cell divisions begin after 4 days in culture from parenchyma cells within and near the procambial tissues of the embryo axis. By day 8 continuous meristematic zones are formed in association with vascular tissues, and DNA synthesis and cell divisions are distributed throughout these zones. No morphological evidence exists to show that these zones consist of proliferating root primordia, which are formed only after the level of 2,4-D falls below some critical concentration. When the concentration of 2,4-D is lowered, the meristematic zones first become dissected and then give rise to many root meristems.
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  • 72
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    Euphytica 32 (1983), S. 217-223 
    ISSN: 1573-5060
    Keywords: Hexaploid triticale ; Secale cereale ; rye ; Triticum aestivum ; wheat ; heterochromatin ; chromosome association
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In the hexaploid triticale (× Triticosecale Wittmack) cultivar Rosner chromosome 2R lacks the prominent heterochromatic bands of both telomeres. This modified 2R chromosome is capable of pairing in a high frequency with wheat chromosomes. It is hypothesized that the accumulation of heterochromatin at the telomeres of rye chromosomes may have contributed to the isolation of the wheat and rye genera by inhibiting pairing between wheat and rye chromosomes.
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  • 73
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    Euphytica 32 (1983), S. 431-438 
    ISSN: 1573-5060
    Keywords: Hordeum vulgare ; barley ; Triticum aestivum ; wheat ; T. turgidum ; durum wheat ; X Triticosecale ; triticale ; salt tolerance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Saline soils are typically very patchy in their salinity. The yield of crops growing on them is similarly patchy. This paper argues that because most of the yield from such soils comes from the least saline areas, the best breeding strategy for improving the overall yield of crops growing on them is to select for high yield on non-saline soils. This conclusion derives from comparing the effects that four different breeding goals, namely: (1) a 10% increase in yield on non-saline soils, (ii) a 20% increase in the threshold salinity that first reduces yield, (iii) a doubling of yield at an electrical conductivity of the saturation extract (ECe) of 20 dS/m and (iv) a combination of (i) and (iii), would have on total yield. The effects of achieving these goals in barley, common wheat, durum wheat and triticale in fields exhibiting different salinities are predicted from actual yields of these species grown on different salinities in the field.
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  • 74
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; kernel color ; protein content ; inheritance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Utilization of high-protein hard red wheat germplasm in breeding high-protein hard white winter wheats for the U.S. Great Plains raised concern regarding possible genetic relationships between kernel color and protein content. Segregating F3 and F4 populations from reciprocal crosses and backcrosses involving high-protein hard red winter wheat cultivar Plainsman V and normal-protein hard white winter wheat line KS75216 were examined. Nonsignificant regression and correlation coefficients in the F3 generations of KS75216/Plainsman V, KS75216//KS75216/Plainsman V and Plainsman V//KS75216/Plainsman V indicated the absence of genetic relationships between kernel color and protein content. Therefore, despite the presence of genes for protein content and kernel color on the same chromosomes (3A, 3B and 3D), kernel color and protein content appeared as independent traits. A small but significant negative relationship between white kernel color and high protein in Plainsman V/KS75216 was attributable to the possible presence of alien genetic material in the parentage of Plainsman V. Chi-square tests indicated that Plainsman V is a mixture of genotypes for kernel color; most genotypes carry two dominant genes for red color and a few carry one or three. Genetic control of grain protein appeared to be complex. Partial dominance for high protein was indicated in the F3 generation but a generally continuous distribution and transgressive segregation also suggested other genes functioned additively. Heritability estimates by parent-offspring (F3-F4) regression were sufficiently high to ensure genetic progress in the selection of high-protein lines in the red x white wheat crosses. We concluded that development of high-protein cultivars is as feasible for white wheats as for red wheats.
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    Euphytica 32 (1983), S. 299-310 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; bread wheat ; ear colour ; glume colour ; genetics ; linkage ; chromosomal location ; geographical distribution ; homeoallelic genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A summary of the brown ear character of bread wheat is presented. In most varieties this character is conditioned by a (semi)dominant gene identified as Rg on 1BS. This gene seems to be widespread, from the viewpoint of evolution, probably because it is an ‘old’ gene. There are no indications that the presence of the gene and hence the presence of a brown ear is advantageous or disadvantageous to the carrier wheat plant. Some linkage relations are described. More research is needed to establish whether all varieties with one gene for brown ear carry Rg, to investigate the varieties with a non-monogenic genetic system and to identify more associations between brown ear and other characters. It should also further be investigated whether within T. spelta another gene for brown ear is present and, if so, whether this gene is on chromosome 1AS and linked to Hgl, the gene for hairy glume.
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    Euphytica 32 (1983), S. 743-748 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; plant physiology ; plant breeding ; Australia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Major changes in the behaviour of the Australian wheat crop over the last one hundred years have been associated with three major gene groupings. The significance of major genes in monitoring response to vernalization, photoperiod and gibberellin, reveals a more optimistic future for breeding programmes where simple genetic and physiologic studies are integrated within those programmes. A revised classification of growth habit is presented in the appendix.
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  • 77
    ISSN: 1573-5036
    Keywords: Acetic acid ; Inhibition ; Wheat growth ; Tillering ; Root growth ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Studies were conducted to determine the effect of and duration of the effect of alliphatic acids on winter wheat (Triticum aestivum L.) seedling root growth, shoot growth, and tillering. Winter wheat seedlings grown in contact with unbuffered solutions of 2 mM or greater acetic or 0.5 mM or greater propionic or butyric acid for 3 days showed decreased root and, in general, shoot growth. Buffering the medium partially alleviated the problem. Removing the seedling from the acid medium and growing it in a nutrient medium resulted in accelerated root growth, compared with the control, while shoot growth was permanently inhibited during this study. Seedling wheat, grown with one root in contact with concentrations of acetic acid ranging from 0–16 mM and the other roots in aliphatic acid-free medium, grew at the same rate as the control. Seedling wheat grown for 3 days in 2 and 4 mM acetic acid medium showed a more rapid formation of the first stem tiller (T1) than did the control. Concentrations of 6 and 8 mM acetic acid appeared to delay T1 tiller formation through the first 18 days after germination, while only 10 mM acetic acid reduced T1 tiller formation by 30% 20 days after germination. The second stem tiller (T2) was not affected by previous exposure to acetic acid. The results of these laboratory studies indicate that short-term exposure of seedling winter wheat to short-chain aliphatic acids can result in permanent shoot and tiller damage and not in permanent root damage as previously thought. These results could explain the poor performance of no-till seeded winter wheat when growing through heavy crop residues that are producing shortchain aliphatic acids during decomposition.
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  • 78
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    Plant and soil 70 (1983), S. 391-402 
    ISSN: 1573-5036
    Keywords: Intercropping Lupins ; Lupinus albus ; Manganese ; Nitrogen ; Phosphorus ; Triticum aestivum ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Significant interactions between wheat and lupins occur below ground and wheat intercropped with lupins has access to a larger pool of available P, Mn and N than has wheat grown in monoculture. This suggests that the wheat is able to take up nutrients produced or made available by lupins grown in association with it.
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  • 79
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    Plant and soil 71 (1983), S. 463-467 
    ISSN: 1573-5036
    Keywords: Anion uptake ; Barley ; Hordeum vulgare ; Mycorrhiza ; Phosphorus ; pH Rhizosphere ; Triticum aestivum ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In two field experiments sown in 1982 to test the effect of vesicular-arbuscular mycorrhizas (VAM) on growth and phosphorus nutrition of (i) spring wheat and spring barley, (ii) winter wheat and winter barley, we measured the concentrations of the major cation (K+, Ca2+, Mg2+ and Na+) and anions (Cl−, SO4 2−, H2PO4 − and NO3 −) in shoot tissue. In all cases the sum of the anion concentrations (ΣA) was increased strongly by mycorrhizal infection but not by P additions, confirming earlier observations2 on spring wheat. The concentration of total cations (ΣA) was generally reduced by P additions, hence P and VAM both reduced the cation excess (ΣC−ΣA) but by different mechanisms. These results suggest that increased uptake of anions by plants with VAM may be a general phenomenom which would have important implications for the elemental composition of crops. The effect may also be manifested by other types of mycorrhizal association.
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  • 80
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    Plant and soil 75 (1983), S. 51-61 
    ISSN: 1573-5036
    Keywords: Aggregate fractions ; Fatty acids ; Long-term rotation ; Triticum aestivum ; Water-stable aggregates
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Three non-replicated, unfertilized, dryland grain rotations—continuous wheat, wheat-fallow, and wheat-wheat-fallow—were established in 1912 on a Dark Brown Chernozemic (Typic Haploboroll) soil. The effect of long-term cropping on the chemical constituents of total water-stable aggregates was assessed. There was a loss in percentage of total water-stable aggregates and a shift in aggregate size distribution with time. Together with an increase in the 100 μm diameter fraction, there was an increase in the sand component of this fraction. These sand particles are probably held together by alkaline-soluble, acid-insoluble organic matter. Organic carbon, polysaccharides, polyuronides, phenols, and chloroform/methanol-extractable organic matter were all associated with the 〉250 μm diameter fractions. Although the aggregates had generally the same suite of aliphatic carboxylic acids, the relative proportions changed with cultivation.
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  • 81
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    Euphytica 32 (1983), S. 593-600 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; bread wheat ; Triticum durum ; durum wheat ; Secale cereale ; inbred lines ; intergeneric crossability ; embryo development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Crossability and embryo development were studied in the crosses of one Triticum aestivum and three T. durum genotypes with nineteen rye inbred lines. Parental wheat and rye genotypes exerted a significant influence on the characters seed set, number of seeds containing embryos and viable plantlets obtained from embryo culture. It was established that the common winter wheat cultivar Götz is of intermediate crossability. The rye inbred lines varied substantially in their capacity to fertilize several wheat genotypes. Interactions between wheats of different crossability classes and their seed set with rye lines were detected. Significant correlations were obtained between seed set and viable plantlets recovered in vitro.
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  • 82
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; protein content ; grain yield ; mixing time ; soil-borne mosaic virus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The wheat (Triticum aestivum L.) cultivar Lancota has genetic potential to produce grain with higher protein content than most other cultivars grown in the hard winter wheat region. It has not consistently expressed full potential for grain protein content outside its area of development. Experiments were conducted to determine genetic variability for grain protein content in Lancota and to utilize that variability to select genotypes with high grain protein content. Approximately 1600 lines were screened to 37 high-protein selections that varied in yield, test weight, flour mixing time, blooming date, height, and reaction to wheat soil-borne mosaic virus (WSBM). Nine promising selections (KS80476, KS80478, KS80480, KS80488, KS80490, KS80491, KS80497, KS80499, and KS80500) had grain protein advantage over Lancota of 0.5 to 1.0% and equalled or exceeded Lancota in yield or test weight. Those selections were resistant to WSBM and satisfactory or better in mixing properties than Lancota. The highest protein selection (KS80496) had a mean protein advantage of 1.5% over Lancota but exhibited a short mixing time of 1 7/8 min. The absence of correlation between some years indicated strong environmental influence on protein content. We concluded that adequate genetic variability existed in the high-grain protein cultivar Lancota to select lines that express the high protein potential better than the original cultivar outside its area of development.
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  • 83
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; linkage drag ; seedling resistance ; Puccinia graminis tritici ; stem rust ; Puccinia recondita ; leaf rust ; Puccinia striiformis ; yellow rust ; stripe rust
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary To determine whether linkage drag had occurred during the breeding of near isogenic lines (NILs) of wheat, 176 lines involving 11 sets of NILs, their recurrent parents and some of their donors were tested for seedling reaction to stem rust (4 races), leaf rust (3 races) and yellow rust (3 races). From the results, six cases were identified in which linkage drag may have played a role. More research is needed to prove clearly that linkage is involved. Nevertheless, the results suggest that linkage drag is a fairly common phenomenon.
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  • 84
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat, glutenin ; high-molecular-weight subunits ; SDS-PAGE ; SDS-sedimentation test ; baking quality selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The high-molecular-weight subunit composition of glutenin is regulated by genes on the long arm of the homoeologous group 1 chromosomes. Evidence is presented that in general the bread-making quality of wheat cultivars containing the subunits 3+10 coded for by chromosome 1D or the subunit 2* coded for by chromosome 1A is higher than that of cultivars containing their allelic counterparts the subunits 2+11 or subunit 1 and the null form respectively. Besides it is shown that the positive effects of the subunits 3+10 and subunit 2* are additive.
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  • 85
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; bread wheat ; Triticum turgidum ; Hordeum vulgare ; barley ; chemotypes ; electrophoresis ; variation ; prolamines ; gliadins ; hordeins ; electrophoregram ; genetic resources
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Polyacrylamide gel electrophoresis (PAGE) of storage proteins (prolamines) was used to screen 64 landraces of wheat and barley from Nepal and the YemenArab Republic and two cultivars for comparison. Altogether 3168 single seeds were examined and the advantages gained by using the vertical slab gel method were recognised. The extent of variation present within populations of landraces could be assessed easily and rapidly using the methods described. Differences in ploidy levels of wheats were detected by PAGE and investigated. Suggestions are made for improvements in sampling strategies in hilly terrain.
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  • 86
    ISSN: 1573-1561
    Keywords: allelopathy ; ferulic acid ; no-tillage ; prickly sida ; common ragweed ; weed control ; 2-methoxy-4-ethenylphenol ; carboxylic acid ; phenol ; morning glory ; Ipomoea lacunosa ; wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract This study was conducted to determine if well-known phytotoxic effects of plant residues on crop growth could also be responsible for observed reductions of certain weed species in no-till cropping systems. An aqueous extract of field-grown wheat (Triticum aestivum L.) reduced the germination and root length of pitted morning glory (Ipomoea lacunosa L.) and common ragweed (Ambrosia artemisiifolia L.). Phytotoxicity was increased by about 70% when bioassays with the wheat extract on morning glory and ragweed were conducted in the presence of light. Phytotoxic substances were extracted from wheat with 2 N NaOH. The hydrolyzed extract was fractionated by thin-layer chromatography (TLC). The compound isolated by TLC having the greatest inhibitory effects on morning glory germination was identified using mass spectrometry and determined to be ferulic acid (4-hydroxy-3-methoxycinnamic acid). Ferulic acid at 5 × 103 M inhibited the germination and root length of morning glory 23 and 82%, respectively, and prickly sida (Sida spinosa L.) with carpels 85 and 82%, respectively. Crabgrass (Digitaria sanguinalis L.) germination was inhibited 100%. Ferulic acid had no effect on ragweed or prickly sida without carpels. Morning glory root and shoot biomass were reduced 52 and 26%, respectively, when morning glory was grown in sand and watered with a 5 × 103 M solution of ferulic acid. Ferulic acid in the presence of prickly sida seed carpels was found to undergo decarboxylation, forming a styrene derivative, 2-methoxy-4-ethenylphenol. The more phytotoxic styrene compound was produced by a bacterium isolated from the carpels of prickly sida seed. The study showed that ferulic acid and other compounds may indeed play a role in reducing the growth of certain weeds in no-tillage cropping systems.
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  • 87
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    Plant and soil 74 (1983), S. 101-109 
    ISSN: 1573-5036
    Keywords: Crop residue ; Root growth ; Shoot growth ; Soil types ; Temperature ; Triticum aestivum ; Wheat germination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Two controlled environment experiments were conducted to examine the germination and early growth of wheat (Triticum aestivum L. cv. Songlen) growing under crop residues of rape, sorghum, field pea and wheat. Additional treamments also included were soil type (Lithic Vertic Ustochrept and Plinthustalf) and temperature (8°C and 24°C to simulate winter and autumn sowing conditions). At low temperature, wheat and sorghum residues produced the most adverse effects on germination with all residues reducing emergence at high temperatures. Shoot lengths were also reduced by most residues at high temperatures whilst root lengths and shoot and root dry weights were unaffected by residue treatments. These results suggest major phytotoxic effects of residues during early growth (up to 14 days after sowing) with, in general, few interactions with soil type or temperature.
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  • 88
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    Plant and soil 74 (1983), S. 229-235 
    ISSN: 1573-5036
    Keywords: Barley ; Hordeum vulgare ; Mineral composition ; Na−K interaction ; Solonetzic soil ; Solonetz-Solod sequence ; Triticum aestivum ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The growth and mineral composition of barley and wheat was measured across sequences of Solonetz and Solod soils. Growth of both species, along with root penetration was reduced on the Solonetz compared to the Solod soil. Mineral composition of the foliage and roots indicated that a Na−K interaction was present for the barley across the Solonetz-Solod sequences. Such factors were considered to be characteristics of soil—plant relationships on Solonetzic soils.
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  • 89
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; chromosome substitution lines ; vernalization ; ear emergence ; basic development rate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The influence of vernalization on days to ear emergence in hexaploid wheat (Triticum aestivum L. em. Thell.) was examined in five Chinese Spring/Thatcher (CS/T) chromosome substitution lines. CS/T 5A and 5D were similar to normal Chinese Spring in days to ear emergence after all periods of vernalization while CS/T 3B and 5B were similar after some, but significantly earlier after other periods of vernalization When compared with Chinese Spring. In both the unvernalized condition and when vernalization did not limit development rate CS/T 7B was faster to ear emergence than Chinese Spring. These results are discussed in relation to the known chromosomal and genetical control of vernalization response.
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  • 90
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    Euphytica 32 (1983), S. 225-230 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; shattering ; awns ; height
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Temporal changes in shattering losses of 14 wheat (Triticum aestivum L.) genotypes were studied in an irrigated environment. The lines ranged in height from 68 to 98 cm; eight lines were fully awned while five were not. Shattered kernels were gathered at weekly intervals for 6 wk; samping commenced at 30 to 40% kernel moisture and continued for 3 wk beyond harvest ripeness (14.5% moisture). Shattering loss, expressed as a percentage of yield, ranged from 3.25 to 17.3% over the 6 wk period. Awnedness was not a factor in shattering susceptibility of the genotypes studied. Both the most and least shattering resistant lines in this study were awnless. There was a nonsignificant correlation between shattering losses and plant height. It was concluded that a single quantitative measurement of shattering loss at or after harvest ripeness (14.5% moisture) would provide an adequate measure of shattering susceptibility.
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  • 91
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    Euphytica 32 (1983), S. 241-255 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; physiology ; grain yield ; inheritance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Post anthesis physiological characters were examined in four genotypes and a diallel set of their progenies. Variation in total carbon uptake by photosynthetic tissue above the flag leaf node was primarily related to leaf area and ear size differences during the early grain filling period. Flag leaf apparent photosynthetic rates during the late grain filling period were closely correlated with flag leaf chorophyl levels. During the period of rapid growth, genotypes differed in the proportion and total quantity of current assimilate translocated to the grain. Total rather than proportional translocation was correlated with grain number and grain yield. Differences in total grain protein were primarily related to total plant nitrogen at anthesis and secondarily to the proportion of this nitrogen translocated to the grain. The level of post anthesis nitrate reductase activity decreased with increasing flag leaf age, but genotypic differences were not closely related to differences in total grain protein contents. Significant general combining ability effects were found for flag leaf chlorophyll levels, flag leaf photosynthetic rates at higher illuminances during the late grain filling period, total plant carbon uptake, total ear carbon uptake, and proportional carbon translocation to the grain. A preponderance of significant general combining ability variances suggests that additive gene action is of particular importance in the inheritance of these physiological characters.
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  • 92
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    Euphytica 32 (1983), S. 257-271 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; bread wheat ; Triticum durum ; durum wheat ; Triticum compactum ; collection ; germplasm ; land-races ; local varieties
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Wheat has traditionally been grown by the Beduin population in the semi-arid (150 to 200 mm, mean total annual rainfall) northern Negev region of Israel. A collection was made in this area (the size of which is 150 km2) from small (0.1 to 0.5ha) fields of mixed wheat, resulting in 1553 collected spikes. Each spike was planted in a 1 m row at Bet Dagan, and grown under favorable conditions. Qualitative and quantitative data were collected from each row. Qualitative data were submitted to hierarchial clustering and the results were compared with published information on the identification, classification and distribution of the land-races of wheat in the Middle East. Triticum durum was represented in 84% of the collection. It was clustered into 22 populations, identified as 11 known varietas of T. durum. They were aggregated into five groups, similar to groups of old varieties recognized by Jacubziner (1932). While 38.5% of the collection consisted of T. durum groups villosa and sinaica, aboriginal to the northern Negev, it included also forms similar to several land-races found in the past in other parts of the Middle East. Each of the populations, and the durum collection as a whole, was very diverse for the quantitatively measured plant attributes. Triticum aestivum was represented in 15.6% of the collection, clustered into six populations. Most of the common wheat accessions were analogous to the old locally grown variety Hirbawi. Triticum compactum was represented in only eight accessions. The collection is now being evaluated as a potential genetic resource for durum wheat breeding.
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  • 93
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; bread wheat ; grains ; dry matter and nitrogen ; distribution within ear ; plant height
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Distribution and accumulation of dry matter (d.m.) and nitrogen (N) were investigated within ears of a semidwarf, a gigas, and a normal wheat genotype grown under controlled environments. A high harvest index was linked with a small pre-anthesis N-storage (and vice versa) but this did not affect N amounts in grains because of compensation by prolonged N uptake during grain filling. N distribution within and between spikelets roughly paralleled that of d.m. Nevertheless, the two processes appeared to be rather independent as became evident from comparisons of the time courses in and between the genotypes. Final distribution of d.m. and N within and between spikelets mainly depended on different rates during the linear phases of accumulation and less on the duration. Maximum of d.m. and N weights per grain as well as maximum of grain number were observed in spikelets below the middle of the ear axis. This ‘submedian dominance’ suggested a pre-anthesis determination of the accumulation potential of grains in different spikelets. N percentage within spikelets disclosed that N accumulation was hampered more than that of d.m. in those positions unfavourable to the latter process. This principal pattern was not affected by genotypes in spite of considerable differences in numbers and weights of grains.
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  • 94
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    Euphytica 32 (1983), S. 575-584 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat seedlings ; Triticum sp. ; Septoria nodorum ; glume blotch disease ; components of partial resistance ; multivariate analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Components of partial resistance of wheat seedlings to Septoria nodorum were measured in a glasshouse. Incubation period, infection frequency, latent period, lesion size, lesion cover, necrosis and unit spore production were recorded on 41 Triticum genotypes. There was no clear evidence of associated variation in components and multivariate analyses were used to elucidate such relationships. Factor analysis indicated that pathogen-induced necrosis, related possiby to toxin susceptibility, and unit spore production were major components of partial resistance to S. nodorum. Principal component analysis was used to characterise genotypes: associated with a continuous general resistance could be a high level of pathogen-induced necrosis or a high unit spore production, but rarely both in the same genotype.
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    Applied microbiology and biotechnology 16 (1982), S. 75-80 
    ISSN: 1432-0614
    Keywords: Immobilization of yeast ; Saccharomyces cerevisiae ; Ethanol production
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary Baker's yeast (Saccharomyces cerevisiae) was immobilized in gels made of prepolymerized, linear, water soluble polyacrylamide, partially substituted with acylhydrazide groups. Gelation was effected by the addition of controlled amounts of dialdehydes (e.g. glyoxal). The immobilized yeasts retained full glycolytic activity. Moreover, the entrapped cells were able to grow inside the chemically corsslinked gel during continuous alcohol production. Glyoxal was found to be the most favourable crosslinking agent for this system. the system employed allowed for the free exchange of substrate and products. The gel surrounding the entrapped cells had no effect on temperature stability profile. On the other hand, substantial enhancement in survival of cells in presence of high ethanol concentrations was recorded for the entrapped yeast. The capability of the immobilized yeast to carry out continuous conversion of glucose to ethanol was demonstrated.
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  • 96
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; Multiple mutants of DNA repair ; Sensitivity to nitrogen mustard and to radiation ; Thermoconditional DNA repair
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Three haploid yeast mutants (snm) sensitive or thermoconditionally sensitive to the DNA cross-linking agent nitrogen mustard (HN2) were crossed with four rad strains representing mutations in the three pathways of DNA dark repair. The resulting haploid double and triple mutant strains were tested for their sensitivity to UV, HN2 and HN1. From the observed epistatic or synergistic interactions of the combinations of mutant alleles we could derive the relation of the SNM1 and SNM2 genes to the postulated repair pathways. Alleles snm1-1 and snml-2 ts were found epistatic to genes of the rad3 group, whereas snm2-1 ts was epistatic to rad6. The snm1 and snm2 mutant alleles interacted synergistically. From these data it is concluded that the SNM1 gene product plays a cross-link specific role in excision repair while the SNM2 gene product may be involved in a system of error-prone repair.
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    Current genetics 6 (1982), S. 163-165 
    ISSN: 1432-0983
    Keywords: Hybridization ; Polyethylene glycol ; Nuclear transfer ; Saccharomyces cerevisiae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Viable hybrids of Saccharomyces cerevisiae were obtained by transfer of isolated diploid nuclei into haploid protoplasts using a polyethylene glycol (PEG) fusion procedure.
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    Current genetics 6 (1982), S. 159-162 
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; Transformation ; Gene subcloning
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have developped a procedure to delimit the boundaries of a cloned gene carried on a DNA fragment as large as 4 to 5 kilobases. The method consists in the following. Two series of limit digest products generated with a tetranucleotide recognition sequence endonuclease and originating from either of the two ends of this DNA segment are tested for their complementing capacity by yeast transformation. The gene is then delimited by the overlap of the two shortest complementing fragments.
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  • 99
    ISSN: 1432-0983
    Keywords: Saccharomyces cerevisiae ; TRP2 gene ; TRP3 gene ; Cloning in yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary This paper describes the isolation of the TRP2 and the TRP3 genes of Saccharomyces cerevisiae. Two pools of plasmids consisting of BamHI and Sa1GI yeast DNA inserts into the bifunctional yeast — Escherichia coli vector pLC544 (Kingsman et al. 1979) were constructed in E. coli and used for the isolation of the two genes by selection for functional complementation of trp2 and trp3 mutations, respectively, in yeast. The TRP2 gene was isolated on a 6.2 kb BamHl and a 5.8 kb Sa1GI yeast DNA fragment which shared an identical 4.5 kb BamHI-SaIGI fragment. The TRP3 gene was located on a 5.2 kb BamHl fragment. By physical, genetic and physiological experiments it could be shown that the cloned yeast DNA fragments contained the whole structural sequences as well as the regulatory regions of the TRP2 and the TRP3 genes.
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    Current genetics 6 (1982), S. 55-61 
    ISSN: 1432-0983
    Keywords: 8 Sequences ; Transposition ; Inversion ; Recombination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A solo δ sequence flanking the 5′ end of the ADHII structural gene, ADR2, can promote a number of DNA rearrangements some of which were investigated in detail. In a selective system haploid mutants were screened in which a solo S sequence flanking ADR2 had been joined to a Ty element. Three different types of events can create such a structure: Reintegration of a Ty sequence at the δ-ADR2 site, inversion of ADR2 and flanking material, and transposition of ADR2 along with 3′ flanking material. The involvement of reciprocal or non-reciprocal exchange mechanisms in creating such events are discussed.
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