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  • Column liquid chromatography  (349)
  • Ultrastructure  (190)
  • evolution
  • stability
  • Springer  (720)
  • Wiley
  • 1985-1989  (720)
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Year
  • 1
    Electronic Resource
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    Springer
    Computing 35 (1985), S. 85-91 
    ISSN: 1436-5057
    Keywords: 65M05 ; 65M10 ; 65M25 ; Second order ; characteristic difference schemes ; quasilinear hyperbolic systems ; stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Description / Table of Contents: Zusammenfassung Wir stellen ein Charakteristikenverfahren zweiter Ordnung für die numerische Lösung der Anfangswertaufgabe von quasilinearen hyperbolischen Systemen vor und beweisen die Stabilität des Verfahrens für Systeme mit konstanten Koeffizienten.
    Notes: Abstract We present two-step, second-order explicit characteristic difference schemes for the numerical solution of initialvalue problems for quasilinear hyperbolic system and show that the method is stable for systems with constant coefficients.
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  • 2
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    Computing 35 (1985), S. 325-344 
    ISSN: 1436-5057
    Keywords: 65L05 ; Numerical analysis ; Nyström methods ; stiff problems ; stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Description / Table of Contents: Abstract The stability of adaptive Nyström-Runge-Kutta procedures is studied for a wide class of nonlinear stiff systems of second order differential equations. We show that for a large class of semi-discrete hyperbolic and parabolic problems the restriction of the stepsize is not due to the stiffness of the differential equation. Furthermore we use the scalar test equation $$y'' = - \omega ^2 y + q \cdot e^{iv(t - t_0 )} $$ to derive conditions which ensure that the numerical forced oscillation is in phase with the analytical forced oscillation. The order of adaptive Nyström-Runge-Kutta methods (with a stability-matrix based on a diagonal Padéapproximation) for which the forced oscillation is in phase with its analytical counterpart cannot be greater than two. This barrier of order is not true forr-stage implicit Nyström methods of orderp=2r.
    Notes: Zusammenfassung Für eine umfangreiche Klasse nichtlinearer steifer Differentialgleichungssysteme zweiter Ordnung wird die Stabilität adaptiver Nyström-Runge-Kutta-Verfahren untersucht. Wir zeigen, daß für eine große Klasse semidiskretisierter hyperbolischer und parabolischer Probleme die Restriktion der Schrittweite unabhängig von der Steifheit des Differentialgleichungssystems ist. Weiterhin verwenden wir die skalare Testgleichung $$y'' = - \omega ^2 y + q \cdot e^{iv(t - t_0 )} $$ und geben Bedingungen dafür an, daß die numerische erzwungene Schwingung mit der analytischen erzwungenen Schwingung in Phase ist. Die Konsistenzordnung adaptiver Nyström-Runge-Kutta-Verfahren (mit einer Stabilitätsmatrix, die auf einer diagnolen Padé-Approximation beruht), für die die erzwungene Schwingung mit ihrem analytischen Gegenstück in Phase ist, kann nicht größer als zwei sein. Diese Ordnungsbarriere gilt nicht fürr-stufige implizite Nyström-Methoden der Ordnungp=2r.
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  • 3
    ISSN: 1435-1536
    Keywords: Emulsions ; stability ; interfacial properties of emulsions ; soybean protein ; glycerides ; stabilised O/W emulsions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The influence of chemically modified 7S fraction of soybean protein (7MSPF), and its partial replacement by mono- and di-glycerides in various ratios, on the rate of drop coalescence in concentrated corn oil-in-water emulsions has been investigated. A total emulsifier concentration of 2.0 % (wt/wt) was used. The minimum drop coalescence rate was achieved when using 1.0% (wt/wt) 7MSPF in conjunction with 0.5% (wt/wt) monoglyceride and 0.5 % (wt/wt) di-glyceride at pH 5.5. At other mono-/di-glycerides and protein/glycerides ratios, and at other pHs, the rate of drop coalescence was higher than when 2.0% (wt/wt) 7MSPF was used. The reduction in drop coalescence rate under these conditions is attributed to association of 7MSPF with the glycerides at the oil-water interface. The influence of protein/glycerides ratio on the viscoelastic properties of mixed interfacial films supports this view.
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  • 4
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    Rheologica acta 26 (1987), S. 119-126 
    ISSN: 1435-1528
    Keywords: Maxwell fluid ; planeCouette flow ; stability ; criticalWeissenberg number
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Physics
    Description / Table of Contents: Abstract The stability behaviour of a Maxwell fluid in a simple plane shear flow for a class of special perturbations is investigated. Necessary and sufficient stability criteria, especially a critical Weissenberg number for the stability (We k ≈ 4) are given. The results of the analysis are in qualitative agreement with experimental observations.
    Notes: Zusammenfassung Es wird das Stabilitätsverhalten eines Maxwell-Fluids in einer einfachen ebenen Scherströmung für eine spezielle Störungsklasse untersucht. Notwendige und hinreichende Stabilitätskriterien sowie eine kritische Weissenbergzahl (We k ≈ 4) werden angegeben. Die Ergebnisse der Analyse stehen mit experimentellen Befunden in qualitativer Übereinstimmung.
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  • 5
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    Fire technology 23 (1987), S. 175-185 
    ISSN: 1572-8099
    Keywords: Fire evaluation ; expert ; consensus ; stability ; reliability ; validity ; scaling measurement
    Source: Springer Online Journal Archives 1860-2000
    Topics: Architecture, Civil Engineering, Surveying
    Notes: Abstract In this article some of the methodological problems associated with using the Delphi technique for the development of a Firesafety Evaluation (Points) Scheme are examined. These problems relate to: questionnaire design, the merits and demerits of Delphi groups versus panel groups, the definition of key terms (such as expert, consensus and stability), the reliability, validity and level of measurement achieved by Likert-type scaling techniques, and the differences in the decisionmaking processes of expert and nonexpert groups.
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  • 6
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    Acta biotheoretica 34 (1985), S. 3-51 
    ISSN: 1572-8358
    Keywords: anthropic principle ; creativity ; evolution ; insight ; manifestation ; mental creativity ; organismal creativity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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  • 7
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    Journal of insect behavior 2 (1989), S. 649-661 
    ISSN: 1572-8889
    Keywords: sociality ; evolution ; Vespidae ; Stenogastrinae ; brood rearing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Stenogastrinae are a subfamily of the Vespidae. The main difference between these and other social wasps (Polistinae and Vespinae) is a jelly-like substance that the Stenogastrinae secrete from the Dufour 's gland and use in many functions of their biology. It is suggested that this substance greatly contributed to the evolution of social life in these wasps by making it possible to nourish the brood with liquid food and store it in the nest, thus favoring also the evolution of the behavioral mechanisms which facilitated interactions between adults. Social organization of the colonies may have been kept at a low level through a basic system of continuous temporary helper replacement, while the evolution of large colonies was restrained, as well as by the poor quality of construction material, low egg-laying capacity and limited production of abdominal substance, imperfect social regulatory mechanisms, and the absence of defensive mechanisms of the colonies against large predators.
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  • 8
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    Journal of dynamics and differential equations 1 (1989), S. 269-298 
    ISSN: 1572-9222
    Keywords: Geometric mechanics ; reduction ; stability ; chaos ; rigid body dynamics ; periodic orbits ; 58F
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract We give a complete bifurcation and stability analysis for the relative equilibria of the dynamics of three coupled planar rigid bodies. We also use the equivariant Weinstein-Moser theorem to show the existence of two periodic orbits distinguished by symmetry type near the stable equilibrium. Finally we prove that the dynamics is chaotic in the sense of Poincaré-Birkhoff-Smale horseshoes using the version of Melnikov's method suitable for systems with symmetry due to Holmes and Marsden.
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  • 9
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    Journal of dynamics and differential equations 1 (1989), S. 299-325 
    ISSN: 1572-9222
    Keywords: Commodity markets ; time delays ; stability ; Hopf bifurcation ; 34K15 ; 45J05 ; 90A16
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract A model for the dynamics of price adjustment in a single commodity market is developed. Nonlinearities in both supply and demand functions are considered explicitly, as are delays due to production lags and storage policies, to yield a nonlinear integrodifferential equation. Conditions for the local stability of the equilibrium price are derived in terms of the elasticities of supply and demand, the supply and demand relaxation times, and the equilibrium production-storage delay. The destabilizing effect of consumer memory on the equilibrium price is analyzed, and the ensuing Hopf bifurcations are described.
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  • 10
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    BIT 26 (1986), S. 93-99 
    ISSN: 1572-9125
    Keywords: Primary 65HO5 ; nonlinear equation ; multiple roots ; multipoint iterative methods ; error constant ; stability ; efficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract A one-parameter family of derivative free multipoint iterative methods of orders three and four are derived for finding the simple and multiple roots off(x)=0. For simple roots, the third order methods require three function evaluations while the fourth order methods require four function evaluations. For multiple roots, the third order methods require six function evaluations while the fourth order methods require eight function evaluations. Numerical results show the robustness of these methods.
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  • 11
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    BIT 27 (1987), S. 424-437 
    ISSN: 1572-9125
    Keywords: 65 L 05 ; 65 L 20 ; stability ; contractivity ; numerical solution of stiff initial value problems in ordinary differential equations ; Runge-Kutta methods ; Rosenbrock methods ; rational Runge-Kutta methods
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract This paper concerns the stability analysis of numerical methods for approximating the solutions to (stiff) initial value problems. Our analysis includes the case of (nonlinear) systems of differential equations that are essentially more general than the classical test equationU′=λU, with λ a complex constant. We explore the relation between two stability concepts, viz. the concepts of contractivity and weak contractivity. General Runge-Kutta methods, one-stage Rosenbrock methods and a notable rational Runge-Kutta method are analysed in some detail.
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  • 12
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    Acta applicandae mathematicae 9 (1987), S. 219-237 
    ISSN: 1572-9036
    Keywords: 34A34 ; 34D99 ; 90A16 ; Nonlinear differential equations ; stability ; growth ; economic dynamics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract This paper analyses the implications of persistent growth upon the stability properties of dynamic models. Besides the traditional concept of asymptotic stability, new stability criteria-strong/weak absolute, strong/weak relative, strong/weak logarithmic stability-are introduced, and global stability conditions for satisfying these criteria are stated for general first-order autonomous differential equations. The conflict between rapidity of growth and the degree of stability is demonstrated. Economic applications of the stability theorems are illustrated within the growth models of Harrod and Solow.
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  • 13
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    Celestial mechanics and dynamical astronomy 47 (1989), S. 333-359 
    ISSN: 1572-9478
    Keywords: symplectic maps ; stability ; normal forms
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract We prove that non resonant isochronous symplectic maps in a neighborhood of an elliptic fixed point are stable for exponentially long times with the inverse of the distance from the fixed point. In the proof we make use of the majorant series method together with an idea for optimizing remainder estimates first applied to Hamiltonian problems by Nekhoroshev.
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  • 14
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    Cellular and molecular life sciences 45 (1989), S. 284-295 
    ISSN: 1420-9071
    Keywords: Annual cycle ; Asclepias ; cardenolide ; Danaidae ; Danaus plexippus ; defense ; ecological chemistry ; evolution ; herbivory ; host plant ; life history ; migration ; milkweed ; monarch butterfly ; overwintering ; predation ; repellent allomone ; sequestration ; storage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Monarch butterflies sequester cardenolides from their larval host plants in the milkweed genusAsclepias for use in defense against predation. Of 108Asclepias species in North America, monarchs are known to feed as larvae on 27. Research on 11 of these has shown that monarchs sequester cardenolides most effectively, to an asymptote of approximately 350 μg/0.1 g dry butterfly, from plants with intermediate cardenolide contents rather than from those with very high or very low cardenolide contents. SinceAsclepias host plant species are distributed widely in space and time across the continent, monarchs exploit them by migration between breeding and overwintering areas. After overwintering in central Mexico, spring migrants east of the Rocky Mountains exploit three predominantAsclepias species in the southern USA that have moderately high cardenolide contents. Monarchs sequester cardenolides very effectively from these species. First generation butterflies are thus well protected against predators and continue the migration north. Across the northern USA and southern Canada most summer breeding occurs on a fourthAsclepias species and in autumn most of these monarchs migrate back to Mexican overwintering sites. The ecological implications of this cycle of cardenolide sequestration for the evolution of monarch migration are discussed.
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  • 15
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    Cellular and molecular life sciences 44 (1988), S. 919-930 
    ISSN: 1420-9071
    Keywords: Heart ; circulation ; blood ; lower vertebrate ; embryology ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In very few instances can the cardiovascular systems of adult ‘lower’ vertebrates serve asdirect models for development in ‘higher’ vertebrates, primarily because numerous evolutionary specializations for preferential distribution of cardiac output between systemic tissues and gas exchange, organs occur in the highly derived circulation of most extant lower vertebrates. Yet, the extensive literature on the cardiovascular anatomy and physiology of aquatic and air breathing fishes, amphibians and reptiles offers important conceptual insights into both patterns and mechanisms of development in birds and mammals. The primary contribution of such studies to the student of developing bird and mammal circulations is the clear demonstration that surprisingly complex hemodynamic function can develop from supposedly ‘simple’ cardiovascular systems typified by incompletely divided heart chambers. Thus, the hemodynamics of embryonic bird and mammal circulations should be determined by measurement, rather than inferred from structure.
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  • 16
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    Cellular and molecular life sciences 41 (1985), S. 24-29 
    ISSN: 1420-9071
    Keywords: Pieridae ; Lepidoptera ; systematics ; evolution ; enzyme electrophoresis ; genetic differentiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Enzyme electrophoretic data show a remarkably high degree of genetic similarity within the European group ofnapi s.l. whereas genetic differences exist at several loci between the European and the North American taxa ofnapi s.l. It is concluded that the European taxa did not differentiate to the species level and form a phylogenetically young group. The North American taxa included in this study are specifically distinct from Europeannapi and separated much earlier. Within these North American taxamarginalis, oleracea andvirginiensis did undergo speciation. The data show a splitting of the genusPieris into three species groups, each genetically differentiated to the same level. The splitting ofPieris into two genera, as suggested by earlier investigators, is not supported here.
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  • 17
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    Cellular and molecular life sciences 41 (1985), S. 1231-1235 
    ISSN: 1420-9071
    Keywords: Sex ; recombination ; evolution ; parasites ; the Tangled Bank ; the Red Queen ; sex-allocation ; sexual selection ; sex determination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
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  • 18
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    Cellular and molecular life sciences 41 (1985), S. 1235-1245 
    ISSN: 1420-9071
    Keywords: Sex ; evolution ; the Tangled Bank ; the Red Queen ; recombination experiments ; genotype × environment interactions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
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  • 19
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    Cellular and molecular life sciences 44 (1988), S. 297-303 
    ISSN: 1420-9071
    Keywords: Biology ; creation science ; creationism ; evolution ; hundredth monkey phenomenon ; paranormal ; pseudoscience
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Public suspicion of science stems from science's challenging of perceptions and myths about reality, and a public fear of new technology. The result is a susceptibility to pseudoscience. In claiming that creation ‘science’ is as valid as evolution the creationists misquote scientists and seek to spread their own ‘scientific’ myths concerning a young age for the earth, an act of creation based on a particular literalist interpretation of the Christian Bible and a single worldwide flood. They use methods of debate and politics, rather than scientific research. A selection of their arguments is examined and the nature of the evidence for evolution is discussed. Problems with the creation ‘science’ model are noted. In the myth of the hundredth monkey phenomenon, original research is misquoted to denigrate scientific research and support sentimental ideas of paranormal events. The misuse of science is seen as damaging to society because it reduces the effective gathering and application of scientific information. However, pseudoscience provides a valuable guide to gaps in public scientific education.
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  • 20
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    Cellular and molecular life sciences 43 (1987), S. 851-860 
    ISSN: 1420-9071
    Keywords: Social insects ; Isoptera ; caste differentiation ; reproductive biology ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The hemimetabolous termites have a very different caste system from social Hymenoptera in which only true imagoes participate in all social tasks. In termites, the imagoes are restricted to reproduction. The termite solidiers have no equivalent in other social insects. They are unique both in their post-embryonic development and their exclusively defensive function. At maturity they keep their molting gland and therefore should be considered as stabilized, differentiated immatures. They appeared monophyletically, early in the evolution of termites. The definition of the worker caste and the current theories explaining its evolution are reexamined after a critical reappraisal of data on post-embryonic development. On the basis of ontogenic, morphologic and functional criteria, we define termite workers as individuals diverging early and irreversibly from the imaginal development, with a morphology typical of their caste but largely of larval appearance, and taking part in most of the social tasks. Such a truly eusocial worker caste is observed in all advanced phyla: Termitidae, Hodotermitidae, andSchedorhinotermes (Rhinotermitidae), but apparently also in one morphologically primitive termite,Mastotermes darwiniensis. This distribution can only be explained by postulating a polyphyletic origin of the worker caste in termites. In all other primitive termites, most of the work is done by temporary helpers (late larvae and nymphs). In these societies, pseudergates are redefined as individuals separating late from the imaginal line, functioning as workers, but not constituting the main working force in the natural society. The particularity of the caste system in termites results from two fundamental peculiarities of their post-embryonic development: -A certain dissociation of molt, growth, and differentiation. -A relative independence in the ontogeny of their different organs.
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  • 21
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    Cellular and molecular life sciences 45 (1989), S. 248-262 
    ISSN: 1420-9071
    Keywords: Sex pheromone ; biosynthesis ; neurobiology ; sensory biology ; orientation behavior ; flight control ; anemotaxis ; genetics ; communication ; olfaction ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Significant progress has been made recently in research on lepidopterous sex pheromones. Advances in understanding the biochemical, neurobiological, and behavioral events that results in both successful and unsuccessful pheromone communication have allowed researchers to gain new insights into the genetic control and evolution of phermone systems.
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  • 22
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    Entomologia experimentalis et applicata 49 (1988), S. 131-140 
    ISSN: 1570-7458
    Keywords: evolution ; monophagy ; polyphagy ; host range ; Vespidae ; caterpillars ; predation ; Mischocyttarus flavitarsus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The narrow host range of insect herbivores is noted, and some of the reasons why specificity has evolved are reviewed. Works indicating the need for new approaches are pointed out including the possibility that generalist predators provide a suitable pressure. Experiments to test the hypothesis that generalists are more vulnerable than specialists to predators are described. They involved a vespid wasp and over thirty species pairs of caterpillars, matched for size and density. Overall, generalists were taken more readily than specialists: some but not all reasons could be detected. The results are discussed in ecological and evolutionary terms.
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  • 23
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    Entomologia experimentalis et applicata 42 (1986), S. 201-212 
    ISSN: 1570-7458
    Keywords: Callosobruchus maculatus ; Bruchidae ; Coleoptera ; behavior ; evolution ; genetic variation ; insect-plant interactions ; oviposition preference
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Vingt-deux lignées deC. maculatus Fab., liées à 6 plantes-hôtes, ont été examinées quant à leurs préférences — sans choix—parmi les graines de 9 légumineuses. Ces préférences se sont révél'ees semblables à celles observées lors de choix binaires; 3 paramètres ont donné les mêmes résultats: nombre moyen d'oeufs pondus par l'ensemble des femelles, nombre moyen d'oeufs émis par l'ensemble de celles acceptant la plante, fréquence des femelles acceptant la plante. L'analyse en composantes principales des préférences pour les 9 plantes a révélé que la majorité des mesures se situe sur les 2 premiers axes, qui ont rendu compte de 89% de la variation. Les performances des lignées sur le premier axe suggèrent qu'il s'agit d'un critère général de préférence. La classification automatique des plantes a montré un aspect général semblable à celui des composantes principales. Par ces 2 méthodes, les plantes ne se sont pas classées nettement selon leurs affinités taxonomiques. Bien que la classification automatique n'ait pas mis en évidence une similarité de comportement chez les insectes liés au même hôte, ceci était apparemment le cas. Les lignées liées à des hôtes présentant de fortes — ou à l'opoosé — de faibles préférences, ont montré différents seuils d'acceptation pour les 9 substrats de ponte. A l'opposé, il n'y a aucune preuve d'altération évolutive de la hiérarchie de préférences. On en a déduit que ces insectes étaient motivés par un petit nombre de stimulus, tels que les allélochimiques et al texture de tégument, pour déceler l'adéquation des substrats potentiels. (La microscopie électronique a suggéré une corrélation entre la texture du tégument et la hiérarchie des préférences de ces populations). La confiance dans les stimulus recueillis contribue à limiter les stratégies évolutives disponibles pour la population. A partir de là, la sélection en faveur —ou contre — des plantes, à l'origine, faiblement préférées, peut conduire, dans la niche de ponte, à l'incorporation — ou à l'exclusion — d'un groupe d'espèces taxonomiquemment éloignées, mais phénétiquement semblables. La probabilité d'altérations importantes de la hiérarchie des préférences paraît extrémement faible.
    Notes: Abstract A survey of 22 strains ofCallosobruchus maculatus associated with six different leguminous hosts indicates extensive genetic differentiation for oviposition preference. Female behavior suggests the existence of two distinct host preference groups among the nine oviposition substrates tested. Strains associated with plants in these two groups show distinct preference profiles. Genetic differentiation of oviposition preference is due to evolutionary modification of the threshold of acceptance of host plants, not to alteration of the preference hierarchy. From principal components analysis of these results, it is likely that very few independent plant traits govern oviposition preference.
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  • 24
    ISSN: 1432-2285
    Keywords: Cambial activity ; Frost hardiness ; Phenology ; Salix ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The ultrastructure of cells in the cambial region of Salix dasyclados Wim. (clone 78056) was studied during the development of winter hardiness and the onset of cambial activity in spring. Plants were grown at relative growth rates (RG) of 8% and 12% respectively, resulting in different nitrogen content in the stems. Frost hardiness of the plants was estimated by standardized freezing tests. Plants with a higher nitrogen status ceased growth later and started re-growth earlier in spring than plants with lower nitrogen content. Differences in ability to withstand low temperatures during autumn and spring were found between plants grown in the two nutrient treatments. During the development of frost hardiness in the autumn, the number of meristematic cells in the cambial region decreased. The cessation of meristematic activity was accompanied by cell wall thickening and ultrastructural changes in the cells. Frost hardiness increased from the ability to survive -6° C in October to survival of -80° C at the beginning of December. From November to February the cambial region comprised a layer of 2–3 thick-walled cells with conspicuous ultrastructural features. Starch accumulated in plastids in September, decreased during November to March and then increased again in accordance with changes of frost hardiness. Onset of cambial activity began between the end of March and the beginning of April, as shown by increased vacuolization of meristematic cells and mitotic activity. By April, the starch content had increased and lipolysis was observed. Frost hardiness had decreased, and plants with low and high nitrogen content were able to survive -15° C and -10° C, respectively. After budburst, all axillary shoot parts were damaged at temperatures below-3° C.
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  • 25
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    Microchimica acta 92 (1987), S. 121-131 
    ISSN: 1436-5073
    Keywords: stability ; molybdenum(VI) ; chelates ; adsorption polarography
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The formation of complexes between Mo(VI) and 8-hydroxy-quinoline (oxine) and four oxine derivatives were investigated by multiwavelength molecular absorption spectrometry, potentiometry, and polarography. The following pKOH- and pKNH- values of the ligands and logK 211-values of the complexes MoO2(OH)2L x− (x=1 or 2) were obtained at 25° C and an ionic strength of 1M(NaClO4): 5,7-dinitro8-hydroxyquinoline 4.59, 〈0, 14.50; 7-nitro-8-hydroxyquinoline-5-sulfonic acid 5.34, 0.41, 15.70; 7-iodo-8-hydroxyquinoline-5-sulfonic acid 6.98, 2.62, 17.65; 8-hydroxyquinoline-5-sulfonic acid 8.33, 4.13, 18.71; and 8-hydroxyquinoline 9.62, 5.28, 19.69. A good linearity was found between logK 211 and the sum of the pK-values of the OH- and NH+-groups. The dependence of the peak current of Mo(VI)-determinations by adsorption polarography of the 7-nitro-8-hydroxyquinoline-5-sulfonate complex of Mo(VI) MoO(OH)3L− can quantitatively be described at pH 0.8–2 using the corresponding pK-values and the log K311 of 18.54±0.03, determined by polarography.
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    K-Theory 1 (1987), S. 185-196 
    ISSN: 1573-0514
    Keywords: Quadratic space ; patching diagram ; projective module ; stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract We prove that every quadratic space of sufficiently large index contains a hyperbolic orthogonal summand.
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    K-Theory 2 (1988), S. 1-355 
    ISSN: 1573-0514
    Keywords: Pseudoisotopy ; stability ; Morse theory
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    Topics: Mathematics
    Notes: Abstract The stability theorem states that the suspension map C(M) → C(M X I) defined on the pseudoisotopy space C(M)=Diff(M X I rel M X O U ∂M X I) of a compact smooth n-manifold M is ∼ n/3-connected. This implies that C(M) has the R~ n/3-homotopy type of the stable pseudoisotopy space P(M) which is related to Waldhausen's algebraic K-theory of spaces by Waldhausen's formula A(X) Ω∞S∞(X+) X B2P(X). This paper gives a detailed proof of the smooth stability theorem following ideas by Hatcher for the proof of a PL stability theorem.
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    Journal of statistical physics 54 (1989), S. 1427-1427 
    ISSN: 1572-9613
    Keywords: Kink propagation ; kink width ; characteristic functions ; stability ; continuum of modes
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    Topics: Physics
    Notes: Abstract We consider the propagation of kinks in an elastic chain in a bistable or multistable potential under the action of a driving force [M. Büttiker and H. Thomas,Phys. Rev. A 37:235 (1988)]. Each element of the chain is subject to a damping force proportional to its velocity. We show that both the propagation velocity of the kinks as a function of the driving field, and the kink width as a function of propagation velocity, are determined by characteristic functions which depend only on the form of the potential. These functions can be found by considering a single particle moving in the upside-down potential of the chain. The general properties of these functions are studied and illustrated by several examples. The stability of these driven kinks is discussed. Interestingly, we find in addition to the expected discrete localized eigenmodes a two-dimensional continuum of oscillatory modes with a localized envelope.
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    Journal of statistical physics 55 (1989), S. 141-156 
    ISSN: 1572-9613
    Keywords: Kosterlitz-Thouless ; Coulomb gas ; hierarchical model ; renormalization group ; screening ; stability ; bifurcation
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    Topics: Physics
    Notes: Abstract A hierarchical version of two-dimensional lattice Coulomb gases is investigated. Forβ〉β c=8π there is a locally stable line of fixed points for the renormalization group (“block charges”) transformations. For $$\beta 〉 \bar \beta _c (\beta _c \leqslant \bar \beta _c \leqslant \tfrac{3}{2}\pi \beta _c )$$ , these fixed points are globally stable. As a consequence we show that there is no screening of external charges for any activity if $$\beta 〉 \bar \beta _c $$ . Atβ c a supercritical bifurcation takes place and we investigate the behavior of the model forβ≲β c to show a weak form of screening.
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    Landscape ecology 3 (1989), S. 43-51 
    ISSN: 1572-9761
    Keywords: Czechoslovakia ; CSSR ; Slovakia ; ecological model ; planning ; landscape management ; stability ; disturbance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Development of the general ecological model (EM) of the CSSR has been included in the state program for environmental policy - the Ecoprogramme of the CSSR — at a scale of 1:1 000 000 for the entire Czechoslovak territory and at a scale of 1:500 000 for the Czech Socialist Republic (CSR) and the Slovak Socialist Republic (SSR). The objective of the first EM stage was to make a survey of spatial differentiation of the major ecological problems of the country. The EM consists of four parts, three analytical and one synthetic. These parts are: a. The ecological state (value) of the current spatial structure of the landscape. b. Ecological stress factors in the landscape. c. Protection of nature and natural resources. From the spatial synthesis of these three groups (from their spatial encounters), the following synthetic group of conditions was obtained: d. Regional ecological problems, a system of ecologically stable areas, environmental stress factors and factors endangering the ecological stability of the landscape, the natural resources and the human environment.
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    Acta mechanica solida Sinica 2 (1989), S. 285-294 
    ISSN: 0894-9166
    Keywords: Liapunov function ; stability ; discrete time system ; decision matrix
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    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract The purpose of this paper is to present a new method for constructing Liapunov function and determining the stability of discrete time systems with a computer on the basis of the similarity transformation theory by directly applying the system matrix of the system under discussion instead of solving the discrete Liapunov's matrix equation.
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    Pure and applied geophysics 130 (1989), S. 743-749 
    ISSN: 1420-9136
    Keywords: Dynamic systems ; multiple equilibrium ; stability
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    Topics: Geosciences , Physics
    Notes: Abstract A set of ordinary differential equations describing a mechanical system subject to forcing and dissipation is considered. A topological argument is employed to show that if all time-dependent solutions of the governing equations are bounded, the equations admitN steady solutions, whereN is a positive odd integer and where at least (N−1)/2 of the steady solutions are unstable. The results are discussed in the context of atmospheric flows, and it is shown that truncated forms of the quasigeostrophic equations of dynamic meteorology and of Budyko-Sellers climate models satisfy the hypotheses of the theorem.
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  • 33
    ISSN: 1420-9071
    Keywords: Spleen ; red pulp ; storage ; release ; morphology ; evolution ; structural characteristics
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    Topics: Biology , Medicine
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    Cellular and molecular life sciences 41 (1985), S. 145-152 
    ISSN: 1420-9071
    Keywords: Spleen ; evolution ; phylogeny ; ontogeny ; non-mammalian spleen ; mammalian spleen ; comparative macromorphology ; comparative micromorphology ; intralienal vessels ; extralienal vessels ; differentiation
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  • 35
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    Cellular and molecular life sciences 42 (1986), S. 624-624 
    ISSN: 1420-9071
    Keywords: Egg pedicel ; evolution ; lacewings ; Hemerobiidae
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    Topics: Biology , Medicine
    Notes: Summary Brown lacewing eggs (Planipennia: Hemerobiidae) glued to the top of hairs of maize leaves can be seen as an intermediary evolutionary step between depositing the egg directly on the substrate (as in most neuropteroid families) and the protective behavioral adaptation of depositing the egg on a long thin pedicel (as e.g. in the Chrysopidae).
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    Cellular and molecular life sciences 44 (1988), S. 735-738 
    ISSN: 1420-9071
    Keywords: Insect ; flight ; evolution
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    Topics: Biology , Medicine
    Notes: Summary The activity patterns of interneurones in the flight systems of dragonflies and mayflies were investigated using standard intracellular recording and staining techniques, and were compared with those of crickets and locusts. The results show several basic similarities in the operation of a central motor pattern generator for flight in all four groups of insects. These similarities can be explained as resulting from conservative evolution of flight pattern generating circuitry within the central nervous system.
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    Cellular and molecular life sciences 44 (1988), S. 633-637 
    ISSN: 1420-9071
    Keywords: Morphogenesis ; evolution ; development
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    Cellular and molecular life sciences 44 (1988), S. 697-700 
    ISSN: 1420-9071
    Keywords: Lizard ; Chamaeleontidae ; Agamidae ; tongue ; feeding ; evolution
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    Topics: Biology , Medicine
    Notes: Summary An incipient form of tongue projection occurs inPhrynocephalus helioscopus, a generalized agamid lizard. We argue that this condition represents a functional intermediate between typical lingual prehension and chamaeleontid tongue projection, and that tongue projection evolved in chameleons by augmentation of ancestral mechanisms still operating in related, generalized lizards.
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    Development genes and evolution 196 (1987), S. 367-371 
    ISSN: 1432-041X
    Keywords: Vitellogenesis ; Bufo marinus oocyte ; Yolk-platelet membrane ; Ultrastructure
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    Topics: Biology
    Notes: Summary Oocytes of the toad Bufo marinus have been studied by means of thin section and particularly freeze-fracture electron microscopy to characterize the cytoplasmic membranes around the yolk organelle, and the storage of yolk material in precursors and platelets. This appears to be a previously unknown type of yolk-platelet formation. During yolk-organelle development from the primordial precursor to the bi-partite fully grown yolk platelet, numerous lipoid droplets are attached to the periphery of the platelet, indicating an intense uptake of lipids. As is typical for amphibians, the fully grown yolk platelet has a crystalline internum covered by a dense osmiophilic externum, and the whole organelle is enveloped by a plasma membrane that shows no direct connection or fusion with endocytotic vesicles. The yolk membrane exhibits few intramembraneous particles (IMPs) at the core areas and some more where it borders fields of lipoid droplets. Here the IMPs show a net-like arrangement in the furrows between adjacent droplets.
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    Development genes and evolution 198 (1989), S. 92-102 
    ISSN: 1432-041X
    Keywords: Vitellogenesis ; Xenopus oocyte ; Yolk-platelet membrane ; Ultrastructure
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    Topics: Biology
    Notes: Summary The yolk platelets ofXenopus laevis have been studied by thin-section and freeze-fracture electron microscopy to characterize the boundary membrane during yolk formation. Throughout vitellogenesis, large yolk platelets are in close contact with smaller nascent yolk organelles. Two types of primordial yolk platelets (I and II) have been discriminated. After membrane fusion these precursors can be completely incorporated into the main body of existing platelets, numerous yolk crystals then merge and form one uniformly stratified core. Lipid droplets are tightly attached to the membrane at all developmental stages of yolk platelets. A direct connection of endoplasmic reticulum to the membranes of yolk platelets was not observed. On freezeetching replicas, yolk-platelet membranes present fracture faces with intramembranous particles (IMP) of various sizes and a heterogeneous distribution of approximately 200–600 IMP/μm2 at the E face, and 1200–2100 IMP/μm2 at the P face. Again, this presentation of the membrane exhibits neither anastomoses to the endoplasmic reticulum, nor caveolae that exclude the uptake of yolk-containing vesicles into these yolk organelles. Proteinaceous yolk platelets tend to fracture along their periphery through the superficial layers.
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    Cellular and molecular life sciences 41 (1985), S. 1285-1296 
    ISSN: 1420-9071
    Keywords: Sex ; sex determination ; sex ratio ; evolution ; animals ; genetics
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    Topics: Biology , Medicine
    Notes: Summary Theories on the evolution of sex determining mechanisms are reviewed for male and female heterogamety, environmental sex determination, and briefly, haplo-diploidy and hermaphroditism. Because of their discrete and well-defined nature, sex determining mechanisms lend themselves to three types of evolutionary questions:what variety occurs and might be expected but does not occur,how do changes occur from one mechanism to another, andwhy do certain changes occur? All three approaches were illustrated for these different sex determining mechanisms. A generality emerging from these studies is that, at the level of selection on the sex ratio, there are no intrinsic problems in evolving from one sex determining mechanism to another: straightforward transitions between different mechanisms exist under various conditions.
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    Cellular and molecular life sciences 41 (1985), S. 1471-1472 
    ISSN: 1420-9071
    Keywords: Phosphoglycerate mutase isozymes ; evolution ; gene duplication
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    Topics: Biology , Medicine
    Notes: Summary The phosphoglycerate mutase isozyme patterns from cyclostomata, cartilaginous and bony fish were analyzed. The observed patterns indicate that a duplication of a single ancestral gene coding for phosphoglycerate mutase took place during the evolution of the gnathostome vertebrates.
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    Cellular and molecular life sciences 42 (1986), S. 788-794 
    ISSN: 1420-9071
    Keywords: Electrostatic complementation ; evolution ; genetic code ; hydropathic complementation ; receptor imprinting
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    Cellular and molecular life sciences 43 (1987), S. 1094-1099 
    ISSN: 1420-9071
    Keywords: Cognitive processes ; metabolism ; evolution ; biomathematical models ; cell proliferation ; cell growth
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    Topics: Biology , Medicine
    Notes: Summary A model for cellular proliferation is described according to which proliferation ensues when metabolism evolves towards commitment to DNA synthesis, and inhibition of proliferation occurs when enzymic interactions are iterated within a few metabolic pathways, another limiting factor being the supply of metabolites. The model successfully describes cellular growth and division as a ‘cognitive process’ based on interaction within enzymic elements and the genome, and affords an explanation in these terms of some empirical phenomena which have previously been understood only as isolated observations.
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    Cellular and molecular life sciences 42 (1986), S. 144-147 
    ISSN: 1420-9071
    Keywords: Ultrastructure ; catalase ; D-amino acid oxidase ; fetal mouse liver ; hepatocytes ; peroxisomes ; muscular dysgenesis
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    Topics: Biology , Medicine
    Notes: Summary In the hepatocytes of ‘normal’ fetal mice from mothers which were carriers of muscular dysgenesis, catalase and D-amino acid oxidase (DAAO) positive as well as negative peroxisomes were observed. DAAO reaction product was occasionally localized in patches around cell membranes and DAAO-positive peroxisomes were frequently observed near mitochondria.
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    Sexual plant reproduction 2 (1989), S. 154-166 
    ISSN: 1432-2145
    Keywords: Helianthus annuus ; Unfertilized ovule culture ; Parthenogenesis ; Ultrastructure ; Proembryo
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    Topics: Biology
    Notes: Summary Electron microscope studies have been conducted on the parthenogenesis induced by in vitro culture of unfertilized ovules of sunflower (Helianthus annuus). In comparison with the state of the egg prior to inoculation, some eggs 5 days after culture show striking ultrastructural changes, which include, among others, nuclear migration, an increase in the number and activity of the organelles, a loss of polarity and wall formation at the chalazal end of the cell. Most of these changes are similar to those that occur normally in the zygote, indicating that parthenogenic development has been triggered in these eggs. Such eggs have been termed activated and are presumed to be capable of undergoing parthenogenesis. The parthenogenic proembryos which result share some features in common with zygotic proembryos. In addition, some parthenogenic proembryos exhibit unique properties not found in zygotic proembryos. These include embryos that consist of two parts differing markedly in density, an inversion of polarity, the frequent occurrence of autophagic vacuoles, the thickening of cell walls, a centripetal growth mode of wall formation, the appearance of an incomplete cell wall, free nuclear division, amitosis and degeneration. We believe that these ultrastructural peculiarities are the effects of in vitro culture.
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    Sexual plant reproduction 2 (1989), S. 193-198 
    ISSN: 1432-2145
    Keywords: Polymorphism ; Ultrastructure ; Pollen grains ; Canna indica L ; Tannin
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    Topics: Biology
    Notes: Summary Our investigations on Canna indica L. indicate that the pollen of this species is polymorphic: there are two types of pollen — a larger type and a comparatively smaller type. Transmission electron microscopy (TEM) revealed the presence of small vacuoles containing tannic substances in the generative cell (GC) of the larger grains: the GC of the mature grain contained a higher quantity of tannins than the GC of the immature grain. Mitochondria, lipid bodies, rough endoplasmic reticulum (RER) and microtubular bundles were present in the cytoplasm of the GC. Numerous mitochondria, lipid bodies and plastids were also present in the vegetative cell (VC), with the mitochondria clustered around the vegetative nucleus. The plastids were observed to be associated with the RER cisterns. During the maturation process, the number of starch grains contained in the plastids decreased.
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    Sexual plant reproduction 1 (1988), S. 208-216 
    ISSN: 1432-2145
    Keywords: Megagametophyte ; Synergids ; Brassica campestris ; Ultrastructure
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    Topics: Biology
    Notes: Summary In Brassica campestris, both synergids of the ovule degenerate before the arrival of the pollen tube. Synergid degeneration does not depend on pollination. At the non-degenerated stage, the synergids are completely filled with a complexly organized cytoplasm containing numerous mitochondria with many cristae, a large number of dictyosomes with many associated vesicles, and a very extensive rough endoplasmic reticulum. The degenerative changes that occur in the cytoplasm of the synergids are characterized by a loss of visibility of the membranes of the endoplasmic reticulum and the simultaneous formation of dense deposits on the surrounding membranes of the mitochondria. Locally, the plasma membranes of the synergids disappear, and some ground plasma of the synergids penetrates into the space between the plasma membranes of the egg cell and the central cell.
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  • 49
    ISSN: 1432-072X
    Keywords: Cyanophyta ; Chroococcidiposis ; Lichenphycobiont ; Cell wall ; “Outer membrane” ; Ultrastructure ; Freeze fracturing/etching ; Patchwork-like leaflet
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    Topics: Biology
    Notes: Abstract Freeze etching studies in a symbiotic and a freeliving strain of Chroococcidiopsis revealed a specific layer in the outer cell wall not described so far from Cyanophyta. The layer showed a complex organisation: The main unit are ribbons, 2–3 nm thick, striated at right angle to the longitudinal axis. They are interwoven to a patchwork-like leaflet. The ribbons are virtually composed of globular particles associated in parallel rows. The cytoplasmic membrane and the cell walls of the symbiotic and the free-living strain were compared.
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    Archives of microbiology 148 (1987), S. 150-154 
    ISSN: 1432-072X
    Keywords: Bacteroides ; Vesicles ; Ultrastructure ; Cellulolytic bacteria ; Rumen
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    Topics: Biology
    Notes: Abstract In 3-day-old cultures of Bacteroides succinogenes grown on filter paper, no cell division was observed. When grown on cellulosic substrate, bacteria exhibited vesicles clustered within cell wall pockets. In 2 day-old filter paper cultures, cells adhered tightly to the substrate. Twenty to 30% of them were dividing. There were cell wall pockets in about 25% of the bacteria, but no vesicles. Whether they adhered to the cellulosic substrate or not, and irrespective of the age of the bacteria, storage polysaccharides were found in the form of dense granules in the cytoplasm. It would appear that vesicles are not essential for cellulose degradation, but are rather a sign of ageing of the cells.
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  • 51
    ISSN: 1432-072X
    Keywords: Methanobacterium formicicum ; Formate dehydrogenase ; F420-hydrogenase ; Immunogold ; Ultrastructure ; Methanogen
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    Topics: Biology
    Notes: Abstract The ultrastructural locations of the coenzyme F420-reducing formate dehydrogenase and coenzyme F420-reducing hydrogenase of Methanobacterium formicicum were determined using immunogold labeling of thin-sectioned, Lowicryl-embedded cells. Both enzymes were located predominantly at the cell membrane. Whole cells displayed minimal F420-dependent formate dehydrogenase activity or F420-dependent hydrogenase activity, and little activity was released upon osmotic shock treatment, suggesting that these enzymes are not soluble periplasmic proteins. Analysis of the deduced amino acid sequences of the formate dehydrogenase subunits revealed no hydrophobic regions that could qualify as putative membrane-spanning domains.
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  • 52
    ISSN: 1432-072X
    Keywords: Gallionella ferruginea ; Thiobacillus ferrooxidans ; Iron bacteria ; Chemolithoautotrophy ; Ultrastructure ; Freeze-etching ; Cell wall organization ; Intracytoplasmic membranes ; Carboxysomes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract By using sodium thioglycolate to dissolve the high amount of excreted stalk material in axenic cultures of the chemolithoautotrophic iron bacterium Gallionella ferruginea, the ultrastructure of Gallionella cells from pure cell suspensions could be studied without any loss of viability or disturbance by dense ferric stalk fibers, and compared with Thiobacillus ferrooxidans, also grown chemolithoautotrophically with ferrous iron as energy source. Both organisms were chemically fixed or freeze-etched. Particular structural differences between these iron-bacteria could be ascertained. G. ferruginea possesses intracytoplasmic membranes and soluble d-ribulose-1,5-bisphosphate-carboxylase, whereas T. ferrooxidans contains carboxysomes but no intracytoplasmic membranes; Gallionella forms poly-β-hydroxybutyrate and glycogen as storage material; T. ferrooxidans produces only glycogen. Both organisms also differ from each other with respect to the freeze fracture behaviour of the cell envelope layers. Whereas the cells of T. ferrooxidans exhibit a characteristic double cleavage, exposing the plasmic fracture face and exoplasmic fracture face of the outer membrane and cytoplasmic membrane, the exceptionally thin multilayered cell envelope of G. ferruginea revealed a particularly intimate association between the layers, resulting in a visualisation of the supramolecular organisation of only the inner fracture face of the cytoplasmic membrane. The results are discussed predominantly in relation to the extremely distinct environments of both organisms.
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  • 53
    ISSN: 1432-072X
    Keywords: Thiothrix sp. ; Beggiatoa sp. ; Sulfideoxidizing ; Polyunsaturated ; Fatty acids ; Inclusions ; Sheath ; Southern California ; Ultrastructure ; Sulfur
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Microscopic examination of the whitish mat that covered the substrata around subtidal hydrothermal vents at White Point in southern California revealed a “Thiothrix-like” bacterium containing sulfur inclusions as the dominant filamentous form in this microbial community. The matlike appearance developed as a result of the closely-packed manner inwhich the basal ends of the filaments were anchored to the substrate. The dominant phospholipid fatty acids of these filaments (16:0, 16:1w7c, 18:0, 18:1w7c) were similar to those recovered from a sample of Beggiatoa isolated from a spring in Florida. Filaments from both sources contained small quantities of C18 and C20 polyunsaturated fatty acids, as well. A larger but less abundant sheathless, filamentous form, which also contained sulfur inclusions and displayed a cell wall structure similar to a previously described Thioploca strain, also colonized the substrata around the subtidal mat. The preservation methods used in the preparation of thin-sections of the subtidal mat material were found to be inadequate for defining some key cellular structures of the large filaments. Nevertheless, the results demonstrate that the filamentous bacteria comprising the microbial mat in the vicinity of the subtidal vents exhibit some of the features of the free-living filamentous microorganisms found in deep-water hydrothermal areas.
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  • 54
    ISSN: 1432-2285
    Keywords: Nuclear crystalloid inclusions ; Olea europaea ; Cytochemistry ; Ultrastructure ; Glycoprotein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The nuclei of mesophyll cells of olive trees contain numerous sizeable crystalloid inclusions. Cytochemical examination using epoxy resin-embedded, semithin-sectioned tissue indicated the presence of proteins and oligoor polysaccharides in these inclusions. Their electron microscopical analysis revealed a crystalline substructure consisting of intersected subunits of high order. The spacing of the lattice fibrils and the angles of intersection were determined and used to establish a model of the unit cell of crystallization. It is suggested that the nuclear crystalloids of olive trees consist of glycoprotein molecules. They differ from the intranuclear crystalloids observed in other species predominantly in the high density of their subunit arrangement.
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    Molecular and cellular biochemistry 84 (1988), S. 155-161 
    ISSN: 1573-4919
    Keywords: aging ; free radicals ; antioxidants ; disease ; evolution ; longevity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Summary Aging is the progressive accumulation of changes with time that are responsible for the ever-increasing likelihood of disease and death. These irreversible changes are attributed to the aging process. This process is now the major cause of death in the developed countries. This fact is obscured by the protean nature of the contributions of this process to the events which terminate life. The aging process may be due to free radical reations. This theory is supported by: 1) studies on the origin and evolution of life; 2) the numerous studies of the effect of ionizing radiation on living systems; 3) life span experiments in which the diet was modified so as to alter endogenous free radical reaction levels; 4) the plausible explanations it provides for aging phenomena; and 5) the growing number of studies which implicate free radical reactions in the pathogenesis of specific diseases. The relationship between aging and diseases involving free radical reactions seems to be a direct one. Modulation of the normal distribution of deleterious free radical reaction-induced changes throughout the body by genetic and environmental differences between individuals results in patterns of change, in some sufficiently different from the normal aging pattern to be recognized as disease. The growing number of ‘free radical’ diseases includes the two major causes of death, cancer and atherosclerosis. It is reasonable to expect on the basis of present data that a judicious selection of diets and antioxidant supplements will increase the healthy, active life span by 5–10 or more years.
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    Biochemical genetics 23 (1985), S. 125-137 
    ISSN: 1573-4927
    Keywords: Triticum timopheevii ; Triticum speltoides ; G genome ; tetraploids ; evolution ; DNA hybridizations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract In vitro DNA:DNA hybridizations and hydroxyapatite thermal-elution chromatography were employed to identify the diploid Triticum species ancestral to the G genome of Triticum timopheevii. Total genomic, unique-sequence, and repeated-sequence fractions of 3H-T. timopheevii DNA were hybridized to the corresponding fractions of unlabeled DNAs of T. searsii, T. speltoides, T. sharonensis, T. longissimum, and T. bicorne. The heteroduplex thermal stabilities indicated that, of the five species examined, T. speltoides was the most closely related to the G genome of T. timopheevii. Thus, T. spelotides appears to be the G-genome donor to T. timopheevii. The thermal stability profiles further indicated that the repeated DNA fractions from the five diploid species and the tetraploid T. timopheevii are more similar than the unique DNA fractions. This indicates that all of these species are closely related and that the sequences which comprise the current repeated fractions in the various species have not undergone any significant change since the formation of various species.
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    Biochemical genetics 27 (1989), S. 17-30 
    ISSN: 1573-4927
    Keywords: λgt11 ; evolution ; hormonal regulation ; myoblasts ; isoelectric focusing ; DNA polymorphism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A cDNA for the mouse carbonic anhydrase, CAIII, has been isolated from a λgt11 expression library. The cloned cDNA contains all of the coding region (777 bp) and both 5′ untranslated (86-bp) and 3′ untranslated (217-bp) sequences. The coding sequence shows 87% homology at the nucleotide level and 91% homology, when amino acid residues are compared, with human CAIII. Protein and mRNA analyses show that CAIII is present at low levels in cultured myoblasts and is abundant in adult skeletal muscle and in liver. The marked sex-related differences in CAIII distribution, described for rat liver, are not seen in the mouse. Restriction fragment length polymorphisms usingTaqI andPstI are described which distinguish betweenMus spretus andMus musculus domesticus.
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  • 58
    ISSN: 1573-4927
    Keywords: cereals ; seed proteins ; amino acid sequences ; genetics ; homology ; evolution
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Haynaldia villosa is a wild grass of the tribe Triticeae, other members of which include the cultivated cereals barley, rye, and wheat. We have made an electrophoretic and chemical characterization of the major seed storage proteins (prolamins) of H. villosa and determined the chromosomal locations of the structural genes for some components using the available wheat/H. villosa chromosome addition lines. As in wheat, barley, and rye, groups of high molecular weight (polymeric), sulfur-poor (monomeric), and sulfur-rich (monomeric γ-type and polymeric) prolamins can be recognized. Most of the components are encoded by genes on chromosome 1 Ha, which is homologous with the chromosomes controlling many of the prolamins in wheat and rye and all of those in barley. In addition, H. villosa also contains α-type sulfur-rich prolamins, previously detected only in wheat and its close relatives. These may be encoded by genes on chromosome 6Ha, which is homologous with the group 6 chromosomes that control the α-type gliadins of wheat. Despite the proposed close relationship between Haynaldia and ryes, no evidence was found for the presence of proteins closely related to the M r 75,000 γ-secalins which are characteristic of wild and cultivated species of Secale.
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  • 59
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    Biochemical genetics 23 (1985), S. 73-88 
    ISSN: 1573-4927
    Keywords: Xenopus ; creatine kinase ; isozymes ; gene duplication and regulation ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Starch gel electrophoresis of creatine kinase (CK) isozymes of Xenopus tropicalis shows that at least two different genes code for CK in this diploid (2n=20) species. These genes seem to be orthologous to the CK-A and CK-C genes of extant crossopterygian fish. Additional isozymes may be interpreted either as products of duplicate genes or, more probably, as epigenetically modified forms of the homodimers AtAt and CtCt, respectively. The originally tetraploid species X. laevis laevis (2n=36), which may have arisen by hybridization of diploid ancestors some 30–40 million years ago, has retained expression of all duplicate CK-A and CK-C genes. Differential expression during ontogenesis (CK-A genes) and in different adult tissues (CK-C genes) indicates that divergence occurred not only with respect to the primary sequence of these duplicate genes, but also with respect to the regulation of their expression. In the interspecific hybrid X. 1. laevis × X. tropicalis, all parental CK genes appear to be expressed simultaneously in the heart. However, several subunit combinations cannot be detected on the zymograms.
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  • 60
    ISSN: 1573-4927
    Keywords: storage protein ; evolution ; napin ; matteuccin
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The ostrich fern,Matteuccia struthiopteris L., contains two globulin spore storage proteins of 2.2 S and 11.3 S, with physical characteristics similar to those of seed storage proteins ofBrassica napus (rapeseed) andRaphanus sativus (radish). By the use of a cloned cDNA that encodes the 1.7 SB. napus storage protein (napin), gene sequences that hybridized with napin were detected in fern nuclear DNA, and a 900-nucleotide homologous mRNA was detected in developing spores.In vitro translation of this fern mRNA produced a 22-kD polypeptide comparable in size to the 21-kD precursor polypeptide identified inBrassica. No hybridizations were observed between theBrassica 12 S clone and either fern DNA or developing spore mRNA.
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    Acta applicandae mathematicae 4 (1985), S. 225-258 
    ISSN: 1572-9036
    Keywords: 92A15 ; Prey ; predator ; competition ; dynamical system ; ordinary differential equation ; phase diagram ; equilibrium ; trajectory ; stability ; bifurcation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract We consider a problem of the dynamics of prey-predator populations suggested by the content of a letter of the biologist Umberto D'Ancona to Vito Volterra. The main feature of the problem is the special type of competition between predators of the same species as well as of different species. Two classes of cases are investigated: a first class in which the behaviour of the predator is ‘blind’ and the second one in which the behaviour is ‘intelligent’. A qualitative analysis of the dynamical systems under consideration is followed by a numerical analysis of the most significant cases.
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    Journal of chemical ecology 12 (1986), S. 1037-1055 
    ISSN: 1573-1561
    Keywords: Drosophila ; Diptera ; Drosophilidae ; yeasts ; cactus ; community ecology ; mutualism ; coadaptation ; evolution ; alkaloids ; fatty acids ; sterols
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The mutualistic interactions of cactophilicDrosophila and their associated yeasts in the Sonoran Desert are studied as a system which has evolved within the framework of their host cactus stem chemistry. Because theDrosophila-yeast system is saphrophytic, their responses are not thought to directly influence the evolution of the host. Host cactus stem chemistry appears to play an important role in determining where cactophilicDrosophila breed and feed. Several chemicals have been identified as being important. These include sterols and alkaloids of senita as well as fatty acids and sterol diols of agria and organpipe cactus. Cactus chemistry appears to have a limited role in directly determining the distribution of cactus-specific yeasts. Those effects which are known are due to unusual lipids of organpipe cactus and triterpene glycosides of agria and organpipe cactus.Drosophilayeast interactions are viewed as mutualistic and can take the form of (1) benefits to theDrosophila by either direct nutritional gains or by detoxification of harmful chemicals produced during decay of the host stem tissue and (2) benefits to the yeast in the form of increased likelihood of transmission to new habitats. Experiments on yeast-yeast interactions in decaying agria cactus provide evidence that the yeast community is coadapted. This coadaptation among yeasts occurs in two manners: (1) mutualistic increases in growth rates (which are independent of the presence ofDrosophila larvae) and (2) stabilizing competitive interactions when growth reaches carrying capacity. This latter form is dependent on larval activity and results in benefits to the larvae present. In this sense, the coadapted yeast community is probably also coadapted with respect to itsDrosophila vector.
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    Journal of chemical ecology 15 (1989), S. 2177-2189 
    ISSN: 1573-1561
    Keywords: Acraea horta ; Lepidoptera ; Acraeinae ; cyclopentenyl cyanoglycoside ; gynocardin ; Kiggelaria africana ; sequestration ; evolution
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract All stages in the life cycle ofAcraea horta (L.) (Lepidoptera: Acraeinae) were found to release hydrogen cyanide (HCN) from their crushed tissues, and the source of cyanogenesis was present in the hemolymph of adults and larvae. Comparison with standards on thin-layer chromatograms (TLC) revealed the presence in adults of gynocardin, a cyclopentenyl cyanoglycoside also produced by the larval food plant,Kiggelaria africana L. (Flacourtiaceae). Analysis of adults reared on plant species (Passifloraceae) containing gynocardin and/or other cyanoglycosides suggested selective uptake of gynocardin by the larvae. This is the first demonstration of a cyanoglycoside, other than the acyclic linamarin and lotaustralin, occurring in Lepidoptera and the first evidence for the storage byAcraea butterflies of a plant-produced allelochemical. Possible implications for the understanding of the evolution of acraeine host choice are discussed.
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    Behavior genetics 17 (1987), S. 513-522 
    ISSN: 1573-3297
    Keywords: Drosophila melanogaster ; courtship ; crowding ; prestimulation ; evolution
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    Topics: Biology , Psychology
    Notes: Abstract The mating times of single males and pairs of males were increased by crowding with virgin females but only at very high densities. Mating times were decreased by the presence of a second male. Quantitative analysis of courtship showed that prestimulation of females in crowded conditions influences mating. The pattern of male courtship was highly consistent across moderate levels of crowding. This suggests thatDrosophila courtships evolved in crowded conditions.
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    Behavior genetics 18 (1988), S. 465-482 
    ISSN: 1573-3297
    Keywords: kin recognition ; animal communication ; signature systems ; birds ; evolution
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    Topics: Biology , Psychology
    Notes: Abstract I develop the argument that for a true kin recognition system to evolve, selection must act on both parties: not only must recognition be favored in the donor of care, but reliable identification must be favored in the potential recipient of the care. This perspective suggests two complementary hypotheses, which I discuss and evaluate with data drawn from studies of birds. According to thesignature adaptation hypothesis, when the sender benefits by reliably identifying itself, selection will act directly on phenotypic characters so as to enhance their signature properties. I summarize our studies on parent-offspring recognition in four species of swallows which are consistent with this hypothesis. In particular, acoustical and perceptual analyses of chick calls show that the calls of colonial swallows are more individually distinctive than are the chick calls of noncolonial swallows. According to theantirecognition hypothesis, when the sender doesnot benefit by reliably identifying itself, selection will act so as to minimize signature characteristics. I suggest two contexts for research on this hypothesis. The first context occurs when parentage is uncertain due to extrapair copulations and/or egg-dumping, and the second context occurs when there is a long period between the onset of chick mobility and chick fledging, as occurs typically in gulls. In both instances, parents will be favored to recognize genetic relatedness of offspring but offspring will be favored to conceal it. To date, data from bird studies are consistent with the prediction that the interests of chicks win out in this situation.
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    Journal of agricultural and environmental ethics 1 (1988), S. 175-192 
    ISSN: 1573-322X
    Keywords: Biodiversity ; biotechnology ; ecology ; ecosystem ; environment ; ethics ; evolution ; genetics ; health ; medicine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Philosophy
    Notes: Abstract The maintenance of biodiversity is urged from many quarters and on grounds ranging from aesthetic considerations to its usefulness, particularly for biotechnology. But regardless of the grounds for preserving biodiversity, writers are generally in agreement that it should be preserved. But, in examining the various references “biodiversity,” such as species diversity, genetic diversity, and habitat diversity, it is apparent that we cannot aim to preserve biodiversityas such, since there are a number of conflicts in any such undertaking. In preserving one aspect of biodiversity, we damage another aspect. Five arguments which attempt to ground our moral concern for biodiversity are reviewed and critiqued, not only for their consistency but also for their power to move us to action. The final section of the paper shows how conflicts in the values of personal and environmental health can impair ethical action and especially policy formation.
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  • 67
    ISSN: 1573-4919
    Keywords: microtubules ; assembly/diassembly ; ATP ; cAMP ; phosphorylation ; stability
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    Topics: Biology , Chemistry and Pharmacology , Medicine
    Notes: Abstract The relevance of protein phosphorylation, transphosphorylation and binding phenomena in the kinetics of the ATP-induced assembly of cycle-purified microtubule protein from mammalian brain were studied. ATP was able to induce the polymerization of microtubules of normal appearance. However, the assembled structures, were unstable and microtubules depolymerized after achievement of a transitory maximum. Cyclic AMP reduced the amplitude of the polymerization maximum in a concentration-dependent manner, correlating with the stimulation of the endogenous phosphorylation reaction. When microtubule assembly was induced by GTP, in the presence of various concentrations of ATP, the slope of the depolymerization phase was found to depend on the concentration of ATP. Fluoride ion inhibited the endogenous phosphorylation reaction and reduced the disassembly rate, in a concentration-dependent manner. Evidence is also presented indicating that ATP did not bind to phosphocellulose-purified tubulin. These results further contribute to indicate that ATP and cyclic AMP, acting coordinately to control the phosphorylation extent of microtubule proteins are important factors to determine microtubule stability within the cell. Some implications of this mechanism for the regulation by cAMP of the initiation of DNA synthesis and mitosis are considered.
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  • 68
    ISSN: 1573-4927
    Keywords: wheat ; gliadins ; gel electrophoresis ; evolution ; genetic analysis
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The gliadin composition of 78 spring durum wheat varieties has been studied by one-dimensional (Al-lactate,pH 3.1) and two-dimensional (first dimension, Al-lactate,pH 3.1; second dimension, sodium dodecyl sulfate-polyacrylamide gel) electrophoresis. Analysis of hybrids has shown that all components of the α zone of gliadin spectra are inherited together as blocks and are, probably, coded for by a cluster of tightly linked genes located on chromosome 6A. Fourteen variants of gliadin blocks have been identified, which can be classified into five families on the basis of component composition. All families but one have analogues among chromosome 6A-controlled blocks of bread wheat. The results indicate that some of the genome A diploid genotypes that were ancestors of durum wheats were also ancestors of bread wheats and that polyploid wheats were produced by repeated allopolyploidization events, as has been suggested earlier.
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  • 69
    ISSN: 1573-4927
    Keywords: wheat ; gliadins ; gel electrophoresis ; evolution ; genetic analysis
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The gliadin composition of 78 spring durum wheat varieties has been studied by one-dimensional (Al-lactate,pH 3.1) and two-dimensional (first dimension, Al-lactate,pH 3.1; second dimension, sodium dodecyl sulfate-polyacrylamide gel) electrophoresis. Analysis of hybrids has shown that all components of the α zone of gliadin spectra are inherited together as blocks and are, probably, coded for by a cluster of tightly linked genes located on chromosome 6A. Fourteen variants of gliadin blocks have been identified, which can be classified into five families on the basis of component composition. All families but one have analogues among chromosome 6A-controlled blocks of bread wheat. The results indicate that some of the genome A diploid genotypes that were ancestors of durum wheats were also ancestors of bread wheats and that polyploid wheats were produced by repeated allopolyploidization events, as has been suggested earlier.
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  • 70
    ISSN: 1573-4927
    Keywords: storage protein ; evolution ; napin ; matteuccin
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The ostrich fern,Matteuccia struthiopteris L., contains two globulin spore storage proteins of 2.2 S and 11.3 S, with physical characteristics similar to those of seed storage proteins ofBrassica napus (rapeseed) andRaphanus sativus (radish). By the use of a cloned cDNA that encodes the 1.7 SB. napus storage protein (napin), gene sequences that hybridized with napin were detected in fern nuclear DNA, and a 900-nucleotide homologous mRNA was detected in developing spores.In vitro translation of this fern mRNA produced a 22-kD polypeptide comparable in size to the 21-kD precursor polypeptide identified inBrassica. No hybridizations were observed between theBrassica 12 S clone and either fern DNA or developing spore mRNA.
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    Behavior genetics 15 (1985), S. 483-497 
    ISSN: 1573-3297
    Keywords: mice ; behavior ; domestication ; drift ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Psychology
    Notes: Abstract Three populations of randomly bredMus musculus, differing in size and generations of isolation, were scored on a battery of 16 behavioral and 4 physical measures. The pattern of population differences in wheel activity, open-field behavior, skin conductance, and shuttle-box behavior is consistent with the operation of drift associated more closely with small population size than with generations of separation. Other possible explanations are less likely.
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  • 72
    ISSN: 1573-3297
    Keywords: Mus domesticus ; housemice ; infant behavior ; diallel analysis ; genetic variance ; heritability ; genetic dominance ; fitness ; domestication ; heterosis ; heterozygote advantage ; selection ; evolution ; ecological niche
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Psychology
    Notes: Abstract A complete diallel cross was generated from six Jax inbred strains ofMus domesticus from diverse origins and a second 6×6 diallel generated from strains derived from a single wild population. During their second day of life, infants from both diallels were tested for latency to orient toward and root beneath mothers and, in a separate test, for latency to attach to mother's nipple. Rooting latency showed a significant additive maternal strain effect but little systematic effect of pup genotype. Nipple attachment latencies exhibited complete genetic dominance favoring rapid attachment, with no maternal effects. Patterns of genetic and environmental influences obtained from the two diallels were highly similar for both behaviors, suggesting that for many traits the requirement that strains be drawn from a common base population may be relaxed.
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    The protein journal 8 (1989), S. 629-646 
    ISSN: 1573-4943
    Keywords: Primary structure ; α-chain ; hemoglobin ; Columbiformes ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Primary structure of hemoglobin of α-chain ofColumba livia is presented. The separation of α-chain was obtained from globin by ion-exchange chromatography (CMC-52) and reversed-phase HPLC (RP-2 column). Amino acid sequence of intact as well as tryptic digested chain was determined on gas-phase sequencer. Structure is aligned homologously with 21 other species. Among different exchanges, positions α24 (Tyr→Leu), α26 (Ala→Gly), α32 (Met→Leu), α64 (Asp→Glu), α113 (Leu→Phe), and α129 (Leu→Val) are unique to pigeon hemoglobin. The various exchanges in α-chain are discussed with reference to evolution and phylogeny. The results show that the order Columbiformes is evolutionarily closer to the order Anseriformes. Since the pigeon is homogeneous, having HbA (αA-chain) and lacks αD-chain, its phylogenetic placement could be established among birds having single hemoglobin components.
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    The protein journal 8 (1989), S. 647-652 
    ISSN: 1573-4943
    Keywords: hemoglobin ; primary structure ; gray partridge ; evolution
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    Topics: Chemistry and Pharmacology
    Notes: Abstract The complete amino acid sequence of the αA-chain of major hemoglobin component from gray partridgeFrancolinus pondacerianus is presented. The major component HbA accounts for 75% of the total hemolysate. Separation of the globin subunits was achieved by ion-exchange chromatography on CM-Cellulose in 8 M urea. The sequence was studied by automatic Edman degradation of the native chain and its tryptic peptides in a gas-phase sequencer. The phylogenetic relationship of Galliformes with other avian orders is discussed.
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    Bioscience reports 7 (1987), S. 471-474 
    ISSN: 1573-4935
    Keywords: evolution ; hormones ; imprinting ; peptides
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Certain non-hormone oligopeptides have a greater imprinting effect on Tetrahymena than others. The imprinting potential is unrelated to the length of the peptide chain, but seems greatly dependent on the amino acid sequence. The direct growth-stimulant action developed by the peptides at the first interaction is unrelated to their imprinting effect.
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    Bioscience reports 8 (1988), S. 585-588 
    ISSN: 1573-4935
    Keywords: plants ; evolution ; transposable elements ; protein evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Transposable elements (TE) are natural constituents of plant genomes. However, their presence only becomes apparent if they become dislodged from their resident positions in the genome and transpore into another gene, thereby inducing a mutation. Such TE-induced mutations are somatically unstable because they revert to wild type and hence reconstitute the expression of the mutated gene. The frequent somatic excision of the TE results in a variegated phenotype. Since this instability is inherited in a Mendelian manner the variegated phenotype is nuclear determined. By this criterion TE have been shown to occur in more than 30 species belonging to different families and genera. Many questions arise when dealing with TE: their structure and functions, and the biological significance of the activity of elements in the differentiation of a normal plant or in the evolution of plant genes.
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  • 77
    ISSN: 1573-5028
    Keywords: multigene family ; sweet potato ; Ipomoea batatas ; storage protein ; sporamin ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sporamin, the major soluble protein of the sweet potato tuberous root, is coded for by a multigene family. Fourty-nine essentially full-length sporamin cDNAs isolated from tuberous root cDNA library have been classified by cross hybridization, restriction endonuclease cleavage pattern and ribonuclease cleavage mapping. All the cDNAs fall into one of the two distinct homology groups, subfamilies A and B, which correspond to the polypeptide classes sporamin A and B, respectively. At least 5 different sequences are detected in both of the 22 sporamin A and 27 sporamin B cDNAs. Comparison of the nucleotide sequences of the coding region of three each of sporamin A and B subfamily members, four from cDNAs and two from genomic clones, indicates that intra-subfamily homologies (94 to 98%) are much higher than inter-subfamily homologies (82 to 84%), and there are deletions or insertions of one or two codons at three locations which characterize each subfamily. Large portions of base substitutions in the coding region accompany amino acid substitutions. In contrast to the coding region, most of the structural differences among the members in the 5′ and 3′ noncoding regions are deletions or insertions.
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  • 78
    ISSN: 1573-5028
    Keywords: chloroplast ; evolution ; Pylaiella littoralis (L.) Kjellm. ; rDNA spacer region
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    Topics: Biology
    Notes: Abstract The DNA segment situated between the 16S and 23S rRNA genes belonging to the plastid genome of the brown alga Pylaiella littoralis (L.) Kjellm. has been sequenced. This small region (322 bp) contains two unsplit tRNA genes separated by 3 bp. A comparison with similar regions from different plants shows that this region has evolved in two different ways according to the place of plants in evolution. In the “primitive” group, this region is reduced in size when compared to prokaryotes. In the other groups, it is considerably enlarged by insertion of repetitive sequences, open reading frames and introns.
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    Journal of mathematical biology 21 (1985), S. 285-298 
    ISSN: 1432-1416
    Keywords: Population dynamics ; coexistence ; mutualism ; persistence ; predator-mediated coexistence ; stability
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    Topics: Biology , Mathematics
    Notes: Abstract We address the question of the long term coexistence of three interacting species whose dynamics are governed by the ordinary differential equations x i = X i f i (i = 1, 2, 3). In order for any theory in this area to be useful in practice, it must utilize as little information as possible concerning the forms of the f i , in view of the great difficulty of determining these experimentally. Here we obtain, under rather general conditions on the equations, a criterion for judging whether the species will coexist in a biologically realistic manner. This criterion depends only on the behaviour near the one or two species equilibria of the two dimensional subsystems, the behaviour there being relatively easy to examine experimentally. We show that with the exception of one class of cases, which is a generalization of a classical example of May and Leonard [21], invasibility at each such equilibrium suitably interpreted is both necessary and sufficient for a strong form of coexistence to hold. In the exceptional case, a single additional condition at the equilibria is enough to ensure coexistence.
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    Journal of mathematical biology 22 (1985), S. 81-104 
    ISSN: 1432-1416
    Keywords: FitzHugh-Nagumo equation ; pulse solution ; stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Mathematics
    Notes: Abstract The FitzHugh-Nagumo equation u t =u xx +f(u)-w, u t =b(u-dw), is a simplified mathematical description of a nerve axon. If the parameters b〉0 and d⩾0 are taken suitably, this equation has two travelling pulse solutions with different propagation speeds. We study the stability of the fast pulse solution when b〉0 is sufficiently small. It is proved analytically by eigenvalue analysis that the fast pulse solution is “exponentially stable” if d〉0, and is “marginally stable” but not exponentially stable if d=0.
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    Journal of mathematical biology 22 (1985), S. 105-115 
    ISSN: 1432-1416
    Keywords: ESS ; evolution ; game dynamics ; population genetics ; sexual populations ; strategies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Mathematics
    Notes: Abstract As an extension of the concept of an evolutionarily stable strategy (ESS) evolutionarily stable sets are introduced, i.e. sets of equilibrium strategies (EQS) which have much of the properties of an ESS. They are primarily used with evolutionary game models that allow a continuum of EQSs, none of which can be an ESS, but also include common ESSs as a special case. For a large class even of nonlinear models it can be shown that the standard dynamics converge towards some equilibrium point in an ES set if started within a neighbourhood of the set. Important applications of ES sets include e.g. mixed-strategist models and evolutionary game models in sexual populations.
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    Theoretical and applied genetics 72 (1986), S. 840-844 
    ISSN: 1432-2242
    Keywords: Chlorophytum comosum ; First pollen mitosis ; Male plastid inheritance ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The behaviour of plastids and mitochondria during the formation and development of the male gametophyte of Chlorophytum comosum has been investigated using electron microscopy. During first pollen mitosis an intracellular polarization of plastids occurs in that the plastids are clustered in the centre of the microspore. The originating generative cell normally lacks plastids. Only in a small number of microspores have plastids been observed near the dividing nucleus of the microspore and later on in the generative cell. These observations agree with the genetic investigations of Collins (1922) on the mode of plastid inheritance which demonstrated a small amount of biparental plastid inheritance in Chlorophytum. The cytological mechanisms underlying plastid polarization during the first pollen mitosis are discussed.
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  • 83
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    Theoretical and applied genetics 74 (1987), S. 177-187 
    ISSN: 1432-2242
    Keywords: Barley ; Grain development ; Mutants ; Ultrastructure ; Genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Eleven Na-azide induced barley shrunken endosperm mutants expressing xenia (sex) were characterized genetically and histologically. All mutants have reduced kernel size with kernel weights ranging from 11 to 57% of the wild type. With one exception, the mutant phenotypes are ascribable to single recessive mutant alleles, giving rise to a ratio of 3∶1 of normal and shrunken kernels on heterozygous plants. One mutant (B10), also monofactorially inherited, shows a gene dosage dependent pattern of expression in the endosperm. Among the 8 mutants tested for allelism, no allelic mutant genes were discovered. By means of translocation mapping, the mutant gene of B10 was localized to the short arm of chromosome 7, and that of B9 to the short arm of chromosome 1. Based on microscopy studies, the mutant kernel phenotypes fall into three classes, viz. mutants with both endosperm and embryo affected and with a non-viable embryo, mutants with both endosperm and embryo affected and with a viable embryo giving rise to plants with a clearly mutant phenotype, and finally mutants with only the endosperm affected and with a normal embryo giving rise to plants with normal phenotype. The mutant collection covers mutations in genes participating in all of the developmental phases of the endosperm, i.e. the passage from syncytial to the cellular endosperm, total lack of aleurone cell formation and disturbance in the pattern of aleurone cell formation. In the starchy endosperm, varying degrees of cell differentiation occur, ranging from slight deviations from wild type to complete loss of starchy endosperm traits. In the embryo, blocks in the major developmental phases are represented in the mutant collection, including arrest at the proembryo stage, continued cell divisions but no differentiation, and embryos deviating only slightly from the wild type.
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  • 84
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    Cell & tissue research 239 (1985), S. 137-140 
    ISSN: 1432-0878
    Keywords: Lanthanum ; Odontoblasts ; Tight junctions ; Tooth pulp ; Ultrastructure ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Odontoblasts are known to be involved in the process of dentinogenesis but it is not clear whether substances may also be deposited in predentine and dentine by passing between these cells. Although tight junctions have been described, it is not clear if they are macular or “leaky” as opposed to continuous or “tight”. In this study use has been made of the permeability of fenestrated capillaries amongst the odontoblasts to deposit the penetrative tracer lanthanum in the interodontoblastic space. This was done by perfusion of anaesthetized rats with physiological solutions containing lanthanum nitrate at 37° C. Immersion fixation of transverse segments of mandibular incisors and examination with an electron microscope showed that lanthanum could permeate 40–50 μm between the odontoblasts to reach the peripheral pulp. Towards the predentine, often less than 10 μm from the capillaries, its progress was abruptly and completely halted by the junctions at the apical ends of the odontoblast cell bodies. Lanthanum was not found in the predentine. The mature secretory odontoblasts in the rat incisor have therefore been shown to be joined by continuous tight junctions. In the process of dentinogenesis this means that all substances deposited in predentine and dentine must arrive by passing through the odontoblasts.
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  • 85
    ISSN: 1432-0878
    Keywords: Corpora allata ; Ultrastructure ; Juvenile hormone ; Rates of synthesis ; Reproductive cycle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Ultrastructure was correlated with rates of juvenile hormone synthesis in corpora allata from females of the viviparous cockroach Diploptera punctata at seven daily intervals during the first vitellogenic cycle. Synthetic activity of the glands was determined by in vitro radiochemical assay before the glands were fixed for electron microscopic analysis. The cycle in rates of juvenile hormone synthesis progressed from about 20 pmol h-1 per gland pair (oocytes 0.60 mm long) to a maximum mean rate of 140 pmol h-1 per pair (oocytes 1.40–1.47 mm long) and declined to about 20 pmol h-1 per pair at ovulation (oocytes about 1.65 mm long). Conspicuous ultrastructural changes occurred with changing synthetic rates. In glands with increasing rates of synthesis, mitochondria showed less electron-dense matrix, greater diameter and more irregular shape. Smooth endoplasmic reticulum changed from easily seen to obscure tubules, networks, and vesicles. Rough endoplasmic reticulum appeared in longer, more curved segments. Newly formed autophagic vacuoles appeared in all glands of highest activity rates. In glands with decreasing rates of synthesis, the mitochondrial matrix became denser, width smaller, and shapes less irregular. Smooth endoplasmic reticulum again appeared tubular and distinct. Golgi complexes were more conspicuous. Rough endoplasmic reticulum in whorls and large numbers of autophagic vacuoles continued to be present.
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  • 86
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    Cell & tissue research 239 (1985), S. 343-347 
    ISSN: 1432-0878
    Keywords: Gastric mucosa ; Surface mucous cell ; Physiological cell loss ; Cell renewal ; Ultrastructure ; Golden hamster
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Fine-structural aspects of physiological cell loss in the gastric mucosa of the golden hamster were observed. As the surface mucous cell ascends along the gastric pit, the cell becomes taller and funnel-like in shape. The interfoveolar cell located at the superficial portion of the gastric pit has many lysosomes and a few lipid droplets in the cytoplasm. The nucleus moves toward the upper region of the cytoplasm, while the Golgi apparatus moves downward toward the infranuclear region. After the rupture of the apical plasma membrane takes place, the lateral and basal plasma membranes of this cell remain in spite of loss of the cell contents. Between the basal plasma membrane of the interfoveolar cell and the capillary endothelium is a thick connective tissue layer characterized by densely packed collagen fibrils. The remaining basal and lateral plasma membranes of the ruptured cell and the thick underlying collagenous layer might play a role in protecting the tissue from potential damage induced by the physiological cell loss.
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  • 87
    ISSN: 1432-0878
    Keywords: Spleen ; Dendritic cells ; Ultrastructure ; Immunization ; Bufo calamita
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the present study comparative aspects of the ultrastructure of the spleen were analyzed in non-immunized and T-dependent antigen-challenged natterjacks, Bufo calamita. Special attention is focused on the role of the non-lymphoid components in the splenic immunoreactivity. Ten days after primary immunization with sheep erythrocytes, splenic lymphoid follicles increase considerably in number and size. By that time, lymphoblasts, medium and large lymphocytes abound in the periphery of the white pulp near the marginal zone. Meanwhile, in the red pulp numerous monocytes migrating across the sinusoidal walls apparently transform into giant, dendritic-like cells. Twenty days after immunization the splenic lymphoid follicles decrease in number, although certain reactivity persists and numerous plasma cells occur in the cell cords and sinusoids of the red pulp. These results are discussed comparatively with those reported in other lower vertebrates.
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  • 88
    ISSN: 1432-0878
    Keywords: Corpora allata ; Ultrastructure ; Precocenes ; Juvenile hormone ; Blattella germanica (Insecta)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Ultrastructural studies on corpora allata (CA) from different stages during the first gonadotropic cycle of the cockroach Blattella germanica have shown well defined changes which have a correspondence with oocyte length, CA volume and juvenile hormone (JH) biosynthesis. The most significant variations concern the mitochondria and the endoplasmic reticulum. Topically applied precocene II (P II) at a dose of 200 ⧎g induced a transient arrest of CA function, although cytotoxic effects were occasionally observed. When CA were maintained in vitro with 10-3 M of P II, a relationship between the time of treatment (3, 6 or 9 h) and the intensity of the effects was apparent. The 9-h treatment led to an irreversible inhibition of JH production which parallels the severe damages observed in the CA (membrane lysis, nuclear pyknosis, vacuolization). Equivalent studies performed with the chroman derivative 3,4-dihydroprecocene II (DHP II) showed that it is less active than P II. Only treatments as severe as 12 h of incubation with a 10-3 M concentration elicited cytotoxic effects which could be due to radical species involved in the in situ oxidative bioactivation of DHP II. Thus, this compound could be regarded as a new type of pro-allatocidin.
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  • 89
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    Cell & tissue research 239 (1985), S. 131-136 
    ISSN: 1432-0878
    Keywords: Capillary permeability ; Lanthanum ; Peripheral nerves ; Tooth pulp ; Ultrastructure ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Experiments were performed to compare the permeability of capillaries supplying the endoneurial environment, which is invested by perineurium, with vascular permeability in the pulp where perineurium is absent. Anaesthetised rats were perfused through the aorta with physiological solutions containing lanthanum nitrate at 37° C. Pieces of inferior alveolar nerve and segments of mandibular incisors were immersion-fixed and transverse sections were examined electron microscopically for the distribution of lanthanum. In the pulp the nerve fibres pass between lanthanum-impermeable arterioles and venules en route to the incisal end. In the peripheral pulp a few capillaries were permeable but the most permeable capillaries lay between the odontoblasts. Pulpal capillary permeability was attributed to the fenestrated endothelium and contrasted with the unfenestrated endoneurial capillaries which were impermeable to lanthanum. Whereas the tight junctions of endoneurial capillaries are known to prevent certain blood-borne substances from entering the endoneurium, it was not clear whether the permeability of the pulpal capillaries, which are distant from the nerve fibres, could affect the nerve fibre environment. No extravasated lanthanum reached the pulpal nerve fibres suggesting that they are not affected. Technically it was not possible to examine the incisal third of the tooth where the situation could be different because the volume of the pulp decreases and capillaries lie closer to the nerve fibres.
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  • 90
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    Cell & tissue research 239 (1985), S. 405-415 
    ISSN: 1432-0878
    Keywords: Testis ; Leydig cell ; FSH ; Morphometry ; Ultrastructure ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effects of FSH on the testicular interstitial tissue of immature hypophysectomized rats were studied by comparing morphological changes in Leydig cells with quantitative changes in interstitial tissue histology using morphometric analysis. Three groups of rats received subcutaneous injections of 0.5 ml saline vehicle or 10 μg rFSH or 20 ng oLH (equivalent to the amount of LH known to contaminate the FSH), twice daily for 7 days. Administration of FSH significantly increased testis weight and stimulated more advanced spermatogenesis compared to saline or LH. Morphometric analysis of testes of LH-treated rats showed a small but significant increase in total interstitial cell volume compared to saline treatment. FSH caused much greater increases in the total volume of interstitial tissue and interstitial cells than either saline or LH and significantly increased the total volume of interstitial fluid by comparison with the other groups. FSH but not saline or LH treatment resulted in a striking hypertrophy of Leydig cells, to produce cells ultrastructurally identical to Leydig cells from adults. Since the target tissue of FSH is the seminiferous epithelium, the observed effects on Leydig cells by FSH treatment suggest that the secretion of factors by the seminiferous tubules may mediate the maturation of Leydig cells.
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  • 91
    ISSN: 1432-0878
    Keywords: Nerve ending ; Tadpole tail ; Ultrastructure ; Acetylcholinesterase ; Metamorphosis ; Rana japonica ; R. catesbeiana
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In anuran tadpole tails, the myelinated motor nerve fibers branch in the myoseptum to innervate both red and white muscle fibers at, or near, their ends. There are no significant ultrastructural differences between the nerve endings of the two types of muscle fibers. Intense acetylcholinesterase reaction product was observed in synaptic clefts and junctional folds, as well as in transverse tubules. As metamorphosis proceeded, the junctional folds of the nerve endings disappeared, however, acetylcholinesterase reaction product was still observed in the synaptic clefts. As muscle fibers began to degenerate, nerve endings began to separate from them. However, after nerve endings were completely separated from the surfaces, degenerated muscle fibers, synaptic and cored vesicles were still well preserved although no acetylcholinesterase reaction product was found. It seems clear that the mechanism of the muscle degeneration in the tadpole tail during metamorphosis is not the result of the degeneration of its nerve endings.
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  • 92
    ISSN: 1432-0878
    Keywords: Synapses ; Intestinal nerve ; Ultrastructure ; Helix pomatia ; Horseradish peroxidase technique
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure of nerve cells and the finestructural organization of synaptic contacts have been investigated in the intestinal nerve in the snail Helix pomatia. Three types of nerve cells, occurring singly or in groups, can be distinguished on the basis of the ultrastructure of their perikaryon and content of granules. The peripheral output of these nerve cells has been verified by retrograde CoCl2 and NiCl2 staining. Both axosomatic and axo-axonic specialized synaptic contacts occur in the intestinal nerve. Presynaptic elements of these synaptic contacts contain 100–120 nm granular vesicles or 120–200 nm neurosecretory-like granules. Following intracellular horseradish peroxidase (HRP) labelling of identified central neurons responsible for peripheral regulatory processes, several labelled axons running toward the periphery can be followed throughout the branches of the intestinal nerve. These labelled axon processes (either primary axon or small collaterals) form specialized synaptic contacts, inside the intestinal nerve, and are always in a postsynaptic position. The occurrence of peripheral axo-somatic and axo-axonic synapses provides a morphological basis for integrative processes taking place in the intestinal nerve (peripheral nervous system) of Helix pomatia.
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  • 93
    ISSN: 1432-0878
    Keywords: Steroid cell ; Ultrastructure ; Steroidogenesis ; Intersexual fish ; Monopterus albus (Teleostei)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructural and biosynthetic characteristics of the steroid cells in the gonad of Monopterus albus have been studied. Ultrastructural features related to steroidogenesis have been identified in the interstitial Leydig cells, Sertoli cells, granulosa cells and thecal cells, and are especially abundant in the Leydig cells during the mid-intersexual phase. Steroidogenic ultrastructures in the Sertoli cells develop only during the maturation of the spermatogenic cysts, whereas in the granulosa and thecal cells, these features become obvious only during the maturation of the large oocytes. EM evidence also suggests a nutritive function for the Sertoli cells and the granulosa cells. Results of in vitro steroidogenic studies, using either testosterone or progesterone as a precursor, show a predominant conversion to androstenedione and 5α-reduced compounds, and suggest a change in biosynthesis from 5α-reduced products to androstenedione during sex reversal. 11-Ketotestosterone (11KT) has been identified, but not 11 β-hydroxytestosterone. Production of 11 KT is high in the late intersexual and the male phases, but a lack of a marked variation in 11KT production between the early and the mid-intersexual phase suggests that this steroid is not a trigger for natural sex reversal in Monopterus.
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  • 94
    ISSN: 1432-0878
    Keywords: Development ; Sea urchin ; Hyaline layer ; Granular layer ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Employing electron-microscopic methods that help retain polyanionic materials, we describe the extracellular coverings of a sea urchin (Lytechinus variegatus) throughout ontogeny. The surface of the embryo is covered by a two-layered cuticle (commonly called the hyaline layer), which in turn is covered by a granular layer. The granular layer is retained after addition of alcian blue to the fixative solutions, and has not been previously described for any sea urchin. After hatching, the granular layer disappears, but the hyaline layer continues to cover most of the larval surface until settlement and metamorphosis. A few days before metamorphosis, the hyaline layer lining the vestibular invagination of the competent pluteus larva is replaced by a three-layered cuticle resembling that of the adult sea urchin. The hyaline layer covering the rest of the larva is evidently lost at metamorphosis during the involution of the general epidermis.
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  • 95
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    Cell & tissue research 239 (1985), S. 693-701 
    ISSN: 1432-0878
    Keywords: Macrophage ; Bone marrow culture ; Heterogeneity ; Ultrastructure ; Mouse
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The development of macrophages in culture from mouse bone marrow was followed for 14 days by light and electron microscopy, ultrastructural cytochemistry, and flow cytometric analysis. By 10 days greater than 97% of the cells in culture were mononuclear phagocytes, and by 12 days greater than 99% were identifiable as macrophages. Ultrastructurally, three subpopulations of mononuclear phagocytes were distinguished based on the appearance of cytoplasmic structures. Early in culture, cells containing large, membrane-bounded vesicles predominated. With increasing time in culture these cells were replaced to varying degrees first by cells that contained vesicles filled with relatively dense, osmiophilic material and, finally, by macrophages that contained granules of various sizes, shapes and staining densities. Cytochemical (peroxidase and acid phosphatase) and colloidal gold uptake studies at the ultrastructural level suggested that many, if not all, of these cytoplasmic structures arose by pinocytosis and subsequent fusion of pinocytic vesicles with lysosomes. Analysis of DNA content of propidium iodide-stained nuclei by flow cytometry, coupled with the examination of cells treated with colchicine to arrest mitosis in metaphase, suggested that cell cycling was a negligible contributor to heterogeneity within cultured populations. Thus, by waiting until 12–14 days after bone marrow cultures were initiated, with partial replenishment of the culture medium at 7 days, heterogeneity could be greatly reduced in cultured macrophage populations. Taking this fact into consideration could help to reduce the variability seen in functional studies of macrophage populations that are less homogeneous.
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  • 96
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    Cell & tissue research 258 (1989), S. 203-210 
    ISSN: 1432-0878
    Keywords: Epididymis ; Histology ; Ultrastructure ; Antechinus stuartii (Marsupialia)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ductus epididymidis of the marsupial mouse Antechinus stuartii was divided into caput, corpus, and caudal regions using several constant morphological landmarks. Tubule diameter and epithelial height increased gradually from caput to cauda. In contrast, the surface area of the lumen of the ductus epididymidis increased to a maximum in the distal caput region, but decreased markedly in the distal cauda in association with characteristic changes in lumen shape (from circular to slit-shaped) and epithelial height. Epithelial cells of the ductus epididymidis were generally similar in structure to those described in other mammalian species. Principal and basal cells were common throughout the epithelium. Clear and mitochondria-rich cells were also identified, but occurred less frequently. Regional variations in cell ultrastructure were observed only in principal cells. Numerous vesicular inclusions occurred in the apical cytoplasm of cells in caput segments, membrane-bounded, electron-dense bodies were common in distal corpus regions, and a brush border of microvilli characterized the luminal surface of principal cells in caudal segments. Sperm index increased in the proximal caput, declined to basal levels in the distal caput and proximal corpus, and then increased to a maximum in segment 9 of the distal corpus and remained at about this level throughout the cauda epididymidis. Nuclear rotation, loss of cytoplasmic droplets, and other sperm maturational changes were observed along the epididymis. Discarded cytoplasmic droplets collected in large masses interspersed between aggregates of spermatozoa throughout the distal regions of the duct. There was no evidence of phagocytosis by principal cells of cytoplasmic droplets. The epididymis of A. stuartii differs from that of other mammals. The unusual caudal region, which has little storage capacity for sperm, is an unusual adaptation in a species in which the male is known to be polygamous.
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    Cell & tissue research 258 (1989), S. 247-257 
    ISSN: 1432-0878
    Keywords: Zinc ; Timm method ; Ultrastructure ; Synapses ; Avian brain ; Domestic fowl
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The brain of young domestic chicks was investigated using a Timm sulfide silver method. Serial Vibratome sections were analyzed under the light microscope, and the localization of zinc-positive structures in selected areas was determined at the ultrastructural level. Both strong and differential staining was visible in the avian telencephalon whereas most subtelencephalic structures showed a pale reaction. The highest staining intensity was found in the nonprimary sensory regions of the telencephalon such as the hyperstriatum dorsale, hyperstriatum ventrale, hippocampus, palaeostriatum augmentatum, lobus parolfactorius and caudal parts of neostriatum. There was an overall gradient of staining intensity in neostriatal areas from rostral to caudal with the heaviest zinc deposits in the caudal neostriatum. Primary sensory projection areas, such as the ectostriatum (visual), hyperstriatum intercalatum superius (visual), nucleus basalis (beak representation), the input layer L2 of the auditory field L and the somatosensory area rostral to field L were selectively left unstained. Fiber tracts throughout the brain were free of zinc deposits except for glial cells. In electron micrographs of stained regions, silver grains were localized in some presynaptic boutons of asymmetric synapses (Gray type I), within the cytoplasm of neuronal somata and sporadically in the nucleus. The possible involvement of zinc in synaptic transmission and other processes is discussed.
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  • 98
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    Cell & tissue research 240 (1985), S. 233-242 
    ISSN: 1432-0878
    Keywords: Lymphoid tissue ; Tonsilla lingualis ; Ultrastructure ; B- and T-lymphocytes ; Macaca fascicularis (Primates, Cercopithecoidea)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Lymphoid organs are highly organized structures made up of different tissue compartments, each with its own specific cell populations. However, the cellular elements of the lingual tonsil, which forms a significant part of Waldeyer's pharyngeal ring, are not yet documented. This study, therefore, describes the fine structure and tissue organization of tonsilla lingualis in Macaca fascicularis. Ten selected crypto-lymphatic units originating from five perfusion-fixed animals were analysed ultrastructurally. Based on the fine-structural elements contained within, the lymphoid tissue of tonsillar units could be subdivided into follicular (germinal centre) and parafollicular areas. The latter contained predominantly small lymphocytes, lymphoblasts resembling T-blasts, plasma cells, macrophages, occasional neutrophils and many reticular cells resembling fibroblasts. A distinct feature of the parafollicular area was the presence of numerous high endothelial (HEV)or postcapillary venules (PCV). The follicular areas contained many small and large lymphoid cells, mitotic cells, plasmablasts, macrophages and specialised reticular cells resembling follicular dendritic cells (FDC) with distinct desmosomal junctions. These observations show that the crypto-lymphatic units of the lingual tonsil are, in fact, organised into distinct B- and T-cell compartments with their own specific lymphoid and accessory cells.
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  • 99
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    Cell & tissue research 240 (1985), S. 223-232 
    ISSN: 1432-0878
    Keywords: Oral mucosa, simian ; Local mucosal immunity ; Minor salivary glands ; Duct/lymphoid follicle assemblies ; Ultrastructure ; Macaca fascicularis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Previous light-microscopic and ultra-immunohistochemical tracer studies revealed the existence of an independent local immune response of the simian oral mucosa. This local response is attributed to the presence of minor salivary gland (MSG) duct-related lymphoid tissue. Semithin sections from a total of 263 Epon-embedded tissue blocks from the labial and buccal mucosae of seven monkeys, Macaca fascicularis, were analysed light-microscopically, and 10 suitable MSG duct/follicle assemblies were investigated ultrastructurally. These duct/follicle assemblies include follicular and parafollicular compartments with distinct fine-structural elements. The follicular area or germinal centre contains numerous small and large lymphoid cells, mitotic figures, plasmablasts, macrophages, and cells resembling the follicular dendritic cells with distinct desmosomal junctions. The parafollicular area, which includes the heavily infiltrated duct wall, contains numerous small lymphocytes, T-lymphoblasts, plasma cells and reticular cells resembling fibroblasts. A distinct feature of this compartment is the presence of high endothelial venules (HEV). The presence of HEV and numerous blast cells, resembling blast-forming T-lymphocytes activated in vitro, in a specific area of the duct/follicle assembly strongly suggests that this area is structurally and physiologically identical to the thymus-dependent area of other lymphoid tissues. In other words, the duct/follicle assemblies of simian MSG contain the various specific fine-structural elements that are suitable for antigen recognition and processing. These elements are distributed in discrete compartments comparable to the B- and T-cell areas of “classical” lymphoid tissue.
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  • 100
    ISSN: 1432-0878
    Keywords: Heart ; Mitochondria ; Perfusion ; Ultrastructure ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Mitochondria isolated from heart tissue after a 1-min perfusion with Hanks medium were found to have significantly lower rates of State-3 respiration and respiratory control ratios compared to mitochondria isolated from non-perfused hearts. Examination of the mitochondrial preparations by electron microscopy revealed that a large proportion of the mitochondria isolated from perfused heart tissue were swollen and broken compared to mitochondria from non-perfused hearts.
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