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  • Occupational Health and Environmental Toxicology  (511)
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  • 1
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 14 (1993), S. 495-501 
    ISSN: 0197-8462
    Keywords: pulsed microwaves ; normothermal conditions ; chromosome aberrations ; micro-nuclei ; SCE ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Cytogenetic analyses were performed on human peripheral blood lymphocytes exposed to 2450 MHz microwaves during 30 and 120 min at a constant temperature of 36.1°C (body temperature). The temperature was kept constant by means of a temperature probe put in the blood sample which gives feedback to a microcomputer that controls the microwave supply. We found a marked increase in the frequency of chromosome aberrations (including dicentric chromosomes and acentric fragments) and micronuclei. On the other hand the microwave exposure did not influence the cell kinetics nor the sister chromatid exchange (SCE) frequency. ©1993 Wiley-Liss, Inc.
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  • 2
    Electronic Resource
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    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 14 (1993), S. 503-520 
    ISSN: 0197-8462
    Keywords: constant temperature ; intracellular recording ; time series ; regression analysis ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: The effects of pulsed microwaves (2.45 GHz, 10 μs, 100 pps, SAR: 81.5 kW/kg peak, 81.5 W/kg average) on membrane input resistance and action potential (AP) interval statistics were studied in spontaneously active ganglion neurons of land snails (Helix aspersa), at strictly constant temperature (20.8±.07°C worst case). Statistical comparison with sham-irradiated neurons revealed a significant increase in the mean input resistance of neurons exposed to pulsed microwaves (P ≪ .05 ). Pulsed microwaves had no visible effect on mean AP firing rate; this observation was confirmed by analysis of interspike intervals (ISIs). Using an integrator model for spontaneously active neurons, we found the net input current to be more variable in neurons exposed to pulsed microwaves. The mean input current was not affected. The standard deviation of ISIs and the autocorrelation of the input current were marginally affected, but these changes were not consistent across neurons. Although the observed effects were less obvious than those reported in other studies, they represent evidence of a direct interaction between neurons and pulsed microwaves, in the absence of macroscopic temperature changes. The data do not suggest a single, specific mechanism for such interaction. © 1993 Wiley-Liss, Inc.
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  • 3
    ISSN: 0197-8462
    Keywords: Key words: UHF ; amplitude modulation ; calcium ions ; contraction ; perfused atrial strips ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Calcium efflux from electrically stimulated, 45Ca2+-preloaded atrial strips of the frog heart was measured from samples of the rinsing perfusate collected at 2-min intervals for 32 min in a continuous perfusion chamber. Contractile force was simultaneously monitored. The specimen chamber was located in a stripline apparatus in which the atrial strips were exposed for 32 min to constant (CW) or amplitude-modulated (AM), 1 GHz electromagnetic (EM) fields at specific absorption rates (SAR) ranging from 3.2 μW/kg to 1.6 W/kg. Amplitude modulation was either at 0.5 Hz, in synchrony with the electrical stimulus applied to the preparation, or at 16 Hz. Neither unmodulated nor 0.5 Hz or 16 Hz modulated 1 GHz waves affected the movement of calcium ions or the contractile force in isolated atrial strips of the frog heart. © 1993 Wiley-Liss, Inc.
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  • 4
    Electronic Resource
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    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 14 (1993), S. 535-544 
    ISSN: 0197-8462
    Keywords: exposure facility ; chronic exposure of rats ; magnetic field ; ELF ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: The facility consists of a 12 × 11.5 × 2.4 m high room containing six sets of exposure apparatus and the other equipment necessary to maintain a pathogen-free system. The apparatus sets produced 5 mT (rms), 0.5 mT, or a sham exposure. The apparatus was arranged in the room to minimize the fringing field of the 5 mT set at the sham position. Each set was 3.85 × 1.80 × 0.66 m in outside dimension, containing 24 cages in the magnetically homogeneous region. The apparatus was designed using Harvey's figure-eight-configuration and generated a horizontal sinusoidal alternating field. In order to save electric power, the coil of the apparatus constituted a 50 Hz LC resonance circuit with a condensor bank to which electric power was supplied to compensate losses. Magnetic flux density was kept constant by controlling the coil current. Although mild steel was used in the skeleton of the building, the fringing flux at the sham was as low as 0.1 to 1 μT. Stainless steel was used for ventilating ducts, racks for the cages, cage covers, feeder baskets, and watering nozzles. The homogeneity of the field was measured to be ± 10% in the animal residence area, and food and water consumption was found to be unaffected by the field. At 5 mT, the coil current was 370 A, and the hollow coil was cooled by a stream of 20°C water to prevent both heat and dew on the coil surface. Vibration and acoustic noise was prevented by fiber reinforced plastic framework of the coil. High harmonic distortion was not observed at the output terminal of the coil driver. The facility has operated without trouble for 2 years. © 1993 Wiley-Liss, Inc.
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  • 5
    Electronic Resource
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    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 14 (1993), S. 545-551 
    ISSN: 0197-8462
    Keywords: electromagnetic fields ; clastogenic effect ; non-ionizing radiation ; extremely low frequency (ELF) fields ; lymphocytes ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Experiments designed to evaluate the synergistic production of clastogenic effects by ionizing radiation and 60 Hz magnetic fields were performed using human lymphocytes from peripheral blood. Following exposure to ionizing radiation, cells were cultured in 60 Hz magnetic fields having field strengths up to 1.4 mT. Cells exposed to both ionizing radiation and 60 Hz magnetic fields demonstrated an enhanced frequency of near tetraploid chromosome complements, a feature not observed following exposure to only ionizing radiation. The results are discussed in the context of a multiple-stage model of cellular transformation, employing both initiating and promoting agents. © 1993 Wiley-Liss. Inc.
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  • 6
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    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 14 (1993), S. 557-557 
    ISSN: 0197-8462
    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 7
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    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 14 (1993), S. 559-561 
    ISSN: 0197-8462
    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 8
    Electronic Resource
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    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 14 (1993) 
    ISSN: 0197-8462
    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 9
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    Bioelectromagnetics 14 (1993), S. 553-556 
    ISSN: 0197-8462
    Keywords: analgesia ; depolarization ; neurogenic inflammation ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: High-intensity pulsed magnetic stimulation (HIPMS) non-invasively depolarizes neurones, which can be deeply embedded in local tissues. Trans- or subcutaneous electrical stimulation can produce analgesia. To test the hypothesis that similar analgesia could be obtained using HIPMS, analgesia was determined in ten blinded subjects following HIPMS. Analgesia was consistently produced in all subjects with long-lasting pain relief occurring in half of the cases. ©1993 Wiley-Liss. Inc.
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  • 10
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    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 15 (1994) 
    ISSN: 0197-8462
    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
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  • 11
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    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 14 (1993), S. i 
    ISSN: 0197-8462
    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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  • 12
    Electronic Resource
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    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 15 (1994), S. 1-1 
    ISSN: 0197-8462
    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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  • 13
    Electronic Resource
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    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 15 (1994), S. 1-2 
    ISSN: 0197-8462
    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 14
    Electronic Resource
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    New York, NY [u.a.] : Wiley-Blackwell
    Bioelectromagnetics 15 (1994), S. 2-2 
    ISSN: 0197-8462
    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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  • 15
    ISSN: 0197-8462
    Keywords: dosimetry ; exposure parameters ; induced currents ; induced electric fields ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: This paper presents material which is intended to assist researchers in identifying and controlling a range of biological, electrical, and other physical parameters that can affect the outcome of in vitro studies with extremely low frequency (ELF) magnetic and electric fields. Brief descriptions of power line magnetic and electric fields are provided and methods for the generation of 60 Hz as well as other ELF fields in the laboratory are surveyed. Methods for calculating and measuring exposure parameters in culture medium are also described. Relating in vitro and internal in vivo exposure conditions across different animal species is discussed to aid researchers in selecting levels of field exposure. The text is purposely elementary, and sometimes brief, with references provided to aid the interested reader in obtaining a fuller understanding of the many topics. Because the range of experimental parameters that can influence the outcome of in vitro studies with ELF fields is so broad, a multidisciplinary approach is normally required to carry out the research. © 1993 Wiley-Liss. Inc.
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  • 16
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    Bioelectromagnetics 15 (1994), S. 3-4 
    ISSN: 0197-8462
    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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  • 17
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    Bioelectromagnetics 15 (1994), S. 5-19 
    ISSN: 0197-8462
    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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  • 18
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    Bioelectromagnetics 15 (1994), S. 53-65 
    ISSN: 0197-8462
    Keywords: intermittents ; transients ; EMF ; electric power ; ground currents ; exposure assessment ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: The current through the residential grounding circuit is an important source for magnetic fields; field variations near the grounding circuit accurately track fluctuations in this ground current. In this paper, a model is presented which permits calculation of the range of these fluctuations. A discrete network model is used to simulate a local distribution system for a single street, and a statistical model to simulate unbalanced currents in the system. Simulations of three-house and ten-house networks show that random appliance operation leads to ground current fluctuations which can be quite large, on the order of 600%. This is consistent with measured fluctuations in an actual house. © 1994 Wiley-Liss, Inc.
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  • 19
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    Bioelectromagnetics 15 (1994), S. 21-32 
    ISSN: 0197-8462
    Keywords: ELF ; EMF ; exposure assessment ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Research that has attempted to examine the relationship between work exposures to magnetic fields and health effects has suffered from limited exposure information. Power-frequency electric and magnetic (EM) field exposures vary substantially between industries, occupations, and individuals. In this study magnetic field data were collected for a variety of occupational categories within an electric utility. The sampling procedures emphasized craft occupations that were presumed to have higher exposures to magnetic fields. The objectives were to provide better exposure information for an ongoing cancer mortality study, examine the relationship between different summary measures of magnetic field exposure, and make available descriptive information useful for exposure reduction and worker education. Principal components analysis (PCA) and Varimax rotation were used to explore the relationships between the different summary measures among all utility workers and among the subset of electrical craft occupations. Discriminant analysis was used to assess summary measures of exposure that differentiated occupational groups. Measurements for 770 days generated a total of 42378 hours of magnetic field data. Eleven summary indices of exposure were calculated for specific occupational groups. These include arithmetic mean, geometric mean, median, 95th and 99th percentiles, and fraction of measurements exceeding .5, 1, 5, 10, and 100 μT. Electrical craft occupations had higher work exposures than non-craft occupations. Electricians and substation operators had the highest exposures among craft occupations.We identified subsets of summary measures that were intercorrelated. The first PCA axis included the geometric mean, median, and the fractions exceeding 0.5 and 1.0 μT. This set of measures also were best at discriminating occupational groups. The relevance of these findings may become more important if consistent associations are found between particular occupations and disease and particular occupations and magnetic field summary measures. Further research is needed to evaluate these exposure assessment findings. © 1994 Wiley-Liss, Inc.
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  • 20
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    Bioelectromagnetics 15 (1994), S. 67-75 
    ISSN: 0197-8462
    Keywords: EEG ; electroencephalography ; LEET ; electromagnetic fields ; sleep ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: The sleep inducing effect of a 15 min treatment with either an active or an inactive Low Energy Emission Therapy (LEET) device emitting amplitude-modulated electromagnetic (EM) fields was investigated in a double-blind cross-over study performed on 52 healthy subjects. All subjects were exposed to both active and inactive LEET treatment sessions, with an interval of at least I week between the two sessions. LEET consists of 27.12 MHz amplitude-modulated (sine wave) EM fields emitted intrabuccally by means of an electrically conducting mouthpiece in direct contact with the oral mucosa. The estimated local peak SAR is less than 10 W/kg in the oral mucosa and 0.1 to 100 mW/kg in brain tissue. No appreciable sensation is experienced during treatment, and subjects are therefore unable to tell whether they are receiving an active or an inactive treatment. In this study the active treatment consisted of EM fields intermittently amplitude-modulated (sine wave) at 42.7 Hz for 3 s followed by a pause of 1 s during which no EM fields were emitted. During the inactive treatment no EM fields were emitted. Baseline EEGs were obtained and 15 min post-treatment EEGs were recorded and analyzed according to the Loomis classification.A significant decrease (paired t test) in sleep latency to stage B2 (-1.78 ± 5.57 min, P = 0.013), and an increase in the total duration of stage B2 (1.15 ± 2.47 min, P = 0.0008) were observed on active treatment as compared with inactive treatment. The deepest sleep stage achieved (B1 to D) following active treatment was also significantly higher than that following inactive treatment (P = 0.040). We conclude 27.12 MHz electromagnetic fields, intermittenly amplitude-modulated at 42.7 Hz, result in a significant sleep inducing effect in healthy subjects. © 1994 Wiley-Liss, Inc.
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  • 21
    ISSN: 0197-8462
    Keywords: exposure assessment ; environmental magnetic fields ; residential magnetic fields ; epidemiological protocol ; wire codes ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: A study was carried out in 1990 to guide the development of a protocol for assessing residential exposures of children to time-weighted-average (TWA) power-frequency magnetic fields. The principal goal of this dosimetry study was to determine whether area (i.e., spot and/or 24 h) measurements of power-frequency magnetic fields in the residences and in the schools and daycare centers of 29 children (4 months through 8 years of age) could be used to predict their measured personal 24-h exposures. TWA personal exposures, measured with AMEX-3D meters worn by subjects, were approximately log-normally distributed with both residential and nonresidential geometric means of 0.10 μT (1.0 mG). Between-subjects variability in residential personal exposure levels (geometric standard deviation of 2.4) was substantially greater than that observed for nonresidential personal exposure levels (1.4). The correlation between log-transformed residential and total personal exposure levels was 0.97. Time-weighted averages of the magnetic fields measured in children's bedrooms, family rooms, living rooms, and kitchens were highly correlated with residential personal exposure levels (r = 0.90). In general, magnetic field levels measured in schools and daycare centers attended by subjects were smaller and less variable than measured residential fields and were only weakly correlated with measured nonresidential personal exposures. The final measurement protocol, which will be used in a large US study examining the relationship between childhood leukemia and exposure to magnetic fields, contains the following elements: normal- and low-power spot magnetic field measurements in bedrooms occupied by subjects during the 5 years prior to the date of diagnosis for cases or the corresponding date for controls; spot measurements under normal and low power-usage conditions at the centers of the kitchen and the family room; 24-h magnetic-field recordings near subjects' beds; and wire coding using the Wertheimer-Leeper method. © 1994 Wiley-Liss, Inc. This article is a US Government work and, as such, is in the public domain in the United States of America.
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  • 22
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    Bioelectromagnetics 15 (1994), S. 77-83 
    ISSN: 0197-8462
    Keywords: pulsed magnetic fields ; PMF ; 2-D PAGE ; protein translation ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Quantitative two-dimensional gel electrophoresis of proteins in E. coli exposed for 60 min to weak, pulsed magnetic fields (1.5 mT peak) show that numerous proteins are both increased and decreased by a factor of 2 or more. An increase in the levels of two proteins, the a subunit of DNA-dependent RNA polymerase and NusA, was confirmed by Western blot analysis. © 1994 Wiley-Liss, Inc.
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  • 23
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    Bioelectromagnetics 15 (1994) 
    ISSN: 0197-8462
    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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  • 24
    ISSN: 0197-8462
    Keywords: azide ; mutagenicity ; magnetic fields ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Forty-eight hours exposure to a two Gauss (0.2 mT) rms 60 Hz time varying sinusoidal electromagnetic field increased the number of azide induced TA100 revertant colonies of Salmonella typhimurium 14% as compared with controls in the ambient 〈 2 milli-Gauss 60 Hz field. In the absence of the electromagnetic field, the numbers of mutant colonies grown within and outside the non-energized coil were nearly identical. Without azide, the number of “spontaneous” mutant colonies forming in the experimental field was not statistically significant from numbers of colonies not exposed to field effects. Experimental temperature variation of 2 °C had little effect on colony formation, and the enhanced production of revertant colonies in the presence of the magnetic field was maintained during continued culture for 5 additional days. © 1994 Wiley-Liss, Inc.
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  • 25
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    Bioelectromagnetics 15 (1994), S. 95-104 
    ISSN: 0197-8462
    Keywords: microwaves ; radial-arm maze ; learning ; memory ; cholinergic systems ; endogenous opioids ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: After 45 min of exposure to pulsed 2450 MHz microwaves (2 μsec pulses, 500 pps, 1 mW/cm2, average whole body SAR 0.6 W/kg), rats showed retarded learning while performing in the radial-arm maze to obtain food rewards, indicating a deficit in spatial “working memory” function. This behavioral deficit was reversed by pretreatment before exposure with the cholinergic agonist physostigmine or the opiate antagonist naltrexone, whereas pretreatment with the peripheral opiate antagonist naloxone methiodide showed no reversal of effect. These data indicate that both cholinergic and endogenous opioid neurotransmitter systems in the brain are involved in the microwave-induced spatial memory deficit. © 1994 Wiley-Liss, Inc.
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  • 26
    ISSN: 0197-8462
    Keywords: noise ; cooperativity ; signal-to-noise ; embryo development ; abnormalities ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Living cells exist in an electrically noisy environment. This has led to the so-called “signal-to-noise” problem whereby cells are observed to respond to extremely-low-frequency (ELF) exogenous fields that are several orders of magnitude weaker than local endogenous fields associated with thermal fluctuations. To resolve this dilemma, we propose that living cells are affected only by electromagnetic fields that are spatially coherent over their surface. The basic idea is that a significant number of receptors must be simultaneously and coherently activated (biological cooperativity) to produce effects on the biochemical functioning of the cell. However, like all physical detection systems, cells are subject to the laws of conventional physics and can be confused by noise. This suggests that a spatially coherent but temporally random noise field superimposed on a coherent ELF signal will defeat the mechanism of discrimination against noise, and any observed field-induced bioeffects would be suppressed. An experimental test of this idea was conducted using morphological abnormalities in developing chick embryos caused by electromagnetic field exposure as the endpoint. At an impressed noise amplitude comparable to the ELF field strength (but roughly one-thousandth of the thermal noise field), the increased abnormality rate observed with only the ELF field present was reduced to a level essentially the same as for the control embryos. © 1994 Wiley-Liss, Inc.
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  • 27
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    Bioelectromagnetics 15 (1994), S. 133-142 
    ISSN: 0197-8462
    Keywords: magnetic field ; neuron ; conductance ; cell volume ; Ca ; water of hydration ; cyclic nucleotide ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Calcium chloride and snail physiological salt solutions were exposed to static magnetic fields (2.3-350 mT), and the physical properties of the solutions as well as their biologic effects were studied. Our preliminary observations show that these fields alter physicochemical properties of CaCl2 solutions and the functional effects of physiological solutions. Experiments on CaCl2 solutions demonstrated field-dependent changes of electrical conductivity, with the magnitude and the direction of conductivity change being a function of both concentration and field intensity. The changes in conductivity were maintained for periods in excess of 1 h after exposure. Conductivity changes were not found after exposure of physiological solutions to static magnetic fields, but changes of biological consequence did occur. Other experiments showed that there were several changes in cellular function observed in ganglia and isolated neurons of Helix pomatia when the perfusing medium was changed from the normal physiologic solution to the same solution after exposure to magnetic fields. These changes include membrane depolarization and increased action potential discharge, reduced uptake of Ca into cells, altered content of cyclic nucleotides in ganglia, and increased volume of isolated cell bodies. A change in hydration of calcium ions may be one of the consequences of magnetic-field exposure, and in physiological solutions this change may have functional consequences. © 1994 Wiley-Liss, Inc.
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  • 28
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    Bioelectromagnetics 2 (1981), S. 279-284 
    ISSN: 0197-8462
    Keywords: 970 MHz ; rats ; long-term exposure ; serum chemistry ; hematology ; lymphocytes ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Rats (N = 16) exposed individually in circularly polarized waveguides to 970-MHz electromagnetic radiation (SAR=2.5 mW/g, 22 h daily for 70 consecutive days) had significantly higher serum levels of triglycerides, albumin, and total protein compared with sham-irradiated controls. No difference was observed in the weights, hematologic profile, or in vitro lymphocyte responses to mitogens between these two groups. The higher serum levels of triglycerides in radiofrequency-radiation-exposed rats suggest a non-specific stress reaction.
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  • 29
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    Bioelectromagnetics 3 (1982), S. 3-8 
    ISSN: 0197-8462
    Keywords: electrical properties ; acoustic properties ; history of biophysics and bioengineering ; nonionizing radiation hazards ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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  • 30
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    Bioelectromagnetics 2 (1981) 
    ISSN: 0197-8462
    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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  • 31
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    Bioelectromagnetics 2 (1981), S. 285-289 
    ISSN: 0197-8462
    Keywords: growth inhibition ; magnetic fields ; ELF ; Escherichia coli ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Cultures of Escherichia coli kept at 0 °C in a phosphate buffer solution were exposed to a sinusoidal weak 60- or 600-Hz magnetic field of strength 2 × 10-3 Tesla. A decrease of more than 40% in bacterial count was observed after a 60-h exposure to the magnetic field. Electron micrographs of exposed bacteria show ruptured cell walls, possibly due to the breaking away of flagella under the influence of the sinusoidally varying electromotive force.
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  • 32
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    Bioelectromagnetics 2 (1981), S. 407-409 
    ISSN: 0197-8462
    Keywords: calcium efflux ; complex permittivity ; amplitude windows ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: The recent suggestion that the pattern of positive and negative results of RF-induced calcium efflux from chick brain tissue, when appropriately scaled, matches at three frequencies is examined. Close scrutiny of this recently reported analysis by Joines and Blackman suggests that the uncertainties in the calculated scaling quantities are too large to permit meaningful conclusions.
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  • 33
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    Bioelectromagnetics 3 (1982), S. ix 
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    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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  • 34
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    Bioelectromagnetics 3 (1982), S. 9-16 
    ISSN: 0197-8462
    Keywords: vibrational emissivity ; nonequilibrium systems ; vibrational spectroscopy ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: An analysis of the boson (phonon and photon) distribution in the presence of a steady-state, nonequilibrium molecular subsystem is presented, providing thermodynamic criteria for the boson chemical-potential term, the generalized vibrational emissivity function, and the Bose type of condensation predicted by Fröhlich.
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  • 35
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    Bioelectromagnetics 3 (1982), S. 45-46 
    ISSN: 0197-8462
    Keywords: Non-thermal biological effects ; electric fields ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: A discussion of the general implication of the existance of non-thermal biological effects of electromagnetic fields is presented.
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  • 36
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    Bioelectromagnetics 2 (1981), S. 247-257 
    ISSN: 0197-8462
    Keywords: nervous system ; rat cerebellum ; nonionizing radiation ; Purkinje cells ; microwaves ; radiofrequency ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: In one experiment, Sprague Dawley rats (16-21 days of gestation) and their offspring were exposed to 100-MHz (CW) electromagnetic radiation at 46 mW/cm2 (SAR 2.77 mW/g) for 4 h/day for 97 days. In another experiment, the pregnant rats were irradiated daily from 17 to 21 days of gestation with 2450-MHz (CW) microwaves at 10 mW/cm2 (SAR 2 mW/g) for 21 h/day. In a third experiment, 6-day-old rat pups were irradiated 7 h/day for five days with 2450-MHz radiation at 10 mW/cm2. Equal numbers of animals were sham irradiated in each group. Quantitative studies of Purkinje cells showed a significant and irreversible decrease in rats irradiated during fetal or fetal and early postnatal life. In animals exposed postnatally, and euthanized immediately after irradiation, significant decrease in the relative number of Purkinje cells was apparent. However, restoration apparently occurred after forty days of recovery.
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    Bioelectromagnetics 2 (1981), S. 305-314 
    ISSN: 0197-8462
    Keywords: microwaves (2.45 GHz CW) ; honeybees ; solar power satellites ; longevity ; food consumption ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Adult honeybees, confined singly or in small clusters, were exposed for 0.5, 6, and 24 hours to 2.45-GHz continuous wave microwave radiation at power densities of 3, 6, 12, 25, and 50 mW/cm2. Following exposure, bees were held in the incubator for 21 days to determine the consumption of sucrose syrup and to observe mortality. No significant differences were found between microwave-treated and sham-treated or control bees.
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  • 38
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    Bioelectromagnetics 2 (1981), S. 341-356 
    ISSN: 0197-8462
    Keywords: microwave hazards ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: By introduction of an additional compartment in the hypothalamic region Stolwijk's thermo-regulatory model has been modified to consider partial heating due to hot spots induced by microwaves. It was found that because of thermoregulatory action, the temperature of the hypothalamus will not increase drastically until the rate of energy deposition exceeds the threshold level of about 50 mW/g. The primary controlling mechanisms are blood flow and sweating. For an energy deposition rate of 10 mW/g in the hypothalamus the increase in blood flow in the skin is negligible and the temperature rise of the hypothalamus as compared with blood temperature is about 0.5 °C. It was found that exposure of the head to electromagnetic radiation, in general, causes a decrease in temperature of the trunk and skin. The results show that while the deposition of energy in the hypothalamus at the rate of 10 mW/g produced significant conductive and convective effects, the same total energy uniformly distributed over the cranial cavity produces less significant effects.
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  • 39
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    Bioelectromagnetics 2 (1981), S. 381-390 
    ISSN: 0197-8462
    Keywords: 60-Hz electric fields ; body weight ; oxygen consumption ; rat ; mouse ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: There have been a number of reports in the literature concerning growth-related changes in various animal species exposed to high-strength electric fields. Many of the laboratories reporting such effects have not documented and controlled for the secondary factors that are associated with generating high-strength electric fields (ie, corona, ozone, harmonic distortion, cage vibration, spark discharge). We have designed an exposure system in which we eliminated or minimized these secondary factors, therefore enabling us to examine only the effects of electric fields per se. Sprague-Dawley rats and Swiss-Webster mice were exposed to 60-Hz electric fields at kV/m for up to four months. In 17 individual experiments, we found a greater number of experiments in which the exposed rats had lower body weights than controls. This trend was not evident in data obtained from 14 individual mouse experiments. In more exhaustive growth studies, we found no significant differences in body weights, organ weights, or O2 consumption between exposed and sham-exposed controls. Our failure to detect any major changes in growth was probably the result of eliminating or minimizing the secondary factors associated with electric field exposure.
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  • 40
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    Bioelectromagnetics 2 (1981), S. 291-303 
    ISSN: 0197-8462
    Keywords: 60-Hz electric fields ; motor activity ; carbon dioxide production ; oxygen consumption ; core body temperature ; mice ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Transient effects of 100-kV/m extremely low frequency electric fields were studied in the white footed deermouse, Peromyscus leucopus. Gross motor activity, carbon dioxide production, oxygen consumption, and core body temperature were monitored before, during, and after intermittent field exposures (four hour-long exposures, at one-hour intervals). Thirty-four mice were exposed in cages with plastic floors floating above ground potential, and 21 mice were exposed in cages with grounded metal floor plates. The first field exposure produced an immediate, transient increase of activity and gas measures during the inactive phase of the circadian cycle. All measures returned to baseline levels before the second exposure and were not significantly changed throughout the remainder of the exposures. The rapid habituation of field-induced arousal suggests that significant metabolic changes will not be measured in experiments in which the interval between exposure and measurement is greater than two hours.
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  • 41
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    Bioelectromagnetics 2 (1981), S. 315-328 
    ISSN: 0197-8462
    Keywords: electric fields ; 60 Hz ; honeybees ; transmission lines ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Honeybee colonies exposed under a 765-kV, 60-Hz transmission line at 7 kV/m show the following sequence of effects: 1) increased motor activity with transient increase in hive temperature; 2) abnormal propolization; 3) impaired hive weight gain; 4) queen loss and abnormal production of queen cells; 5) decreased sealed brood; and 6) poor winter survival. When colonies were exposed at 5 different E fields (7, 5.5, 4.1, 1.8, and 0.65-0.85 kV/m) at incremental distances from the line, different thresholds for biologic effects were obtained. Hive net weights showed significant dose-related lags at the following exposures: 7 kV/m, one week; 5.5 kV/m, 2 weeks; and 4.1 kV/m, 11 weeks. The two lowest exposure groups had normal weight after 25 weeks. Abnormal propolization of hive entrances did not occur below 4.1 kV/m. Queen loss occurred in 6 of 7 colonies at 7 kV/m and 1 of 7 at 5.5 kV/m, but not below. Foraging rates were significantly lower only at 7 and 5.5 kV/m. Hive weight impairment and abnormal propolization occur at lower E-field intensity than other effects and limit the “biological effects corridor” of the transmission line to approximately 23 m beyond a ground line projection of each outer phase wire. Intrahive E fields of 15-100 kV/m were measured with a displacement current sensor. Step-potential-induced currents up to 0.5 μA were measured in an electrically equivalent bee model placed on the honeycomb in a hive exposed at 7 kV/m. At 1.8 kV/m body currents were a few nanoamperes, or two orders of magnitude lower, and these colonies showed no effects. E-field versus electric shock mechanisms are discussed.
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  • 42
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    Bioelectromagnetics 2 (1981), S. 357-370 
    ISSN: 0197-8462
    Keywords: rat ; electrocardiogram ; stationary magnetic fields ; T-wave augmentation ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: A field strength dependent increase in the amplitude of the T-wave signal in the rat electrocardiogram (ECG) was observed during exposure to homogeneous, stationary magnetic fields. For 24 adult Sprague-Dawley and Buffalo rats of both sexes, the T-wave amplitude was found to increase by an average of 408% in a 2.0 Tesla (1 Tesla = 104 Gauss) field. No significant magnetically induced changes were observed in other components of the ECG record, including the P wave and the QRS complex. The minimum field level at which augmentation of the T wave could be detected was 0.3 Tesla. The magnetically induced increase in T-wave amplitude occurred instantaneously, and was immediately reversible after exposure to fields as high as 2.0 Tesla. No abnormalities in any component of the ECG record, including the T wave, were noted during a period of 3 weeks following cessation of a continuous 5-h exposure of rats to a 1.5-Tesla field. The heart rate and breathing rate of adult rats were not altered during, or subsequent to, application of fields up to 2.0 Tesla. The effect of animal orientation within the field was tested using juvenile rats 3-14 days old. The maximum increase in T-wave amplitude was observed when subjects were placed with the long axis of the body perpendicular to the lines of magnetic induction. These experimental observations, as well as theoretical considerations, suggest that augmentation of the signal amplitude in the T-wave segment of the ECG may result from a superimposed electrical potential generated by aortic blood flow in the presence of a stationary magnetic field.
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    Bioelectromagnetics 2 (1981), S. 403-406 
    ISSN: 0197-8462
    Keywords: electric fields ; power-frequency ; 60 Hz ; dosimetry ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Calculated electric-field strengths averaged over the body surfaces of grounded humans, swine, rats, horses, and cattle exposed to vertical, uniform, power-frequency electric fields are presented. To produce the same average fields over the body surfaces of grounded animals, as that experienced by a grounded man exposed to an unperturbed vertical field of 10 kV/m, the following unperturbed fields are required: swine, 19 kV/m; rat, 37 kV/m; horse, 18 kV/m; cow, 18 kV/m.
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  • 44
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    Bioelectromagnetics 3 (1982), S. 29-43 
    ISSN: 0197-8462
    Keywords: dielectric properties ; permittivity ; conductivity ; microemulsions ; water of hydration ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: We summarize the results of several of our recent studies on the dielectric properties of protein solutions, tissues, and nonionic microemulsions at microwave frequencies extending to 18 GHz. The data in all cases are analyzed using the Maxwell mixture theory to determine the dielectric properties of the suspending water and the amount and dielectric properties of the water of hydration associated with the suspended phase. The dielectric data from the protein solutions and tissues are broadly consistent with the results of previous studies at UHF frequencies; they indicate hydration values in the range of 0.4-0.6 g water/g protein. There is evidence of a dielectric relaxation process occurring at low-GHz frequencies that can be attributed in part to dielectric relaxation of the “bound” water in the system. The remaining solvent water appears to have dielectric properties close to, if not precisely the same as, those of pure water. The average relaxation frequency of the suspending water in the microemulsions is reduced from that of pure water, evidently reflecting an average of that of the water of hydration (∼5-6 GHz) and that of pure water. This reduced average relaxation frequency implies an increased average viscosity of the water and (by Walden's rule) accounts for the unexpectedly low ionic conductivity of the preparations.
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  • 45
    ISSN: 0197-8462
    Keywords: microwaves ; radiation ; Japanese quail ; immunity ; leukocytes ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Japanese quail, Coturnix coturnix japonica, eggs were subjected to 2.45-GHz CW microwave radiation at 5 mW/cm2 (SAR = 4.03 mW/g) during the first 12 days of embryogeny. Following hatching the exposed embryos, as well as nonexposed controls, were reared to 22 weeks of age. Humoral immune potential, as indicated by comparable anti-CRBC antibody, IgM and IgG, levels at 0, 4, and 7 days postimmunization in both exposed and control quail was not affected significantly. However, cell-mediated immune potential, measured by the reaction to intradermal injection of phytohemagglutinin-P in the wing web, was reduced in the exposed females, but not in the exposed males. Additionally, total leukocyte numbers and absolute circulating numbers of lymphocytes, monocytes, and heterophils were increased significantly only in the exposed females. These data show that exposure of Japanese quail during embryogenesis reduced cell-mediated immune potential and induced a general leukocytosis in females.
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  • 46
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    Bioelectromagnetics 2 (1981), S. 329-340 
    ISSN: 0197-8462
    Keywords: electric fields ; 60 Hz ; biologic effects ; membrane potentials ; recovery ; Pisum sativum ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Roots of Pisum sativum L. were chronically exposed in aqueous inorganic nutrient medium to 60-Hz electric fields between 140 and 490 V/m (growth medium conductivity ∼ 0.08 S/m). The growth rate, meristematic mitotic index, and growth rate recovery of the roots were determined. At 140 V/m there was no perturbation in growth rate or mitotic index. At 430 V/m the growth rate and the mitotic index were reduced. The mitotic index had a maximum depression (∼ 55% of control), which occurred at 4 h. The depression in growth rate was immediate and constant over time. When roots were exposed to an electric field at 430 V/m for 2 days, the growth rate was depressed by about 40%. When the field was terminated, the growth rate steadily increased and was almost normal after 5 days. At 490 V/m root growth rate was almost completely arrested. According to these results, there is a narrow range of induced membrane potentials that span the range from slightly altered to almost completely arrested growth rates.
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    Bioelectromagnetics 2 (1981), S. 371-380 
    ISSN: 0197-8462
    Keywords: electric fields ; 60 Hz ; pineal gland ; circadian rhythm ; melatonin ; 5 methoxytryptophol ; acetyl transferase ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: As a component of studies to search for effects of 60-Hz electric field exposure on mammalian endocrine function, concentrations of melatonin, 5-methoxytryptophol, and serotonin-Nacetyl transferase activity were measured in the pineal glands of rats exposed or sham-exposed at 65 kV/m for 30 days. In two replicate experiments there were statistically significant differences between exposed and control rats in that the normal nocturnal increase in pineal melatonin content was depressed in the exposed animals. Concentrations of 5-methoxytryptophol were increased in the pineal glands of the exposed groups when compared to shamexposed controls. An alteration was also observed in serotonin-N-acetyl transferase activity, with lower levels measured in pineal glands from exposed animals.
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    Bioelectromagnetics 2 (1981), S. 391-402 
    ISSN: 0197-8462
    Keywords: Static electric field ; exposure systems ; animal caging ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: The temporal variation of a static electric field inside an animal cage was investigated with a newly developed small, simple field meter. The field inside the cage was found to be highly dependent on the surface conductivity of the dielectric material. As the surface of the cage became dirty because of animal occupancy, the static electric field inside it became considerably smaller from the moment the field was turned on. Clean cages also modified the static electric field inside them, the field decaying from an initial to a much lower value over several hours. The mechanism of field attenuation for both cases is surface leakage. Surface leakage for a clean cage takes place much more slowly than for a dirty cage. This was confirmed by measuring DC insulation resistance. To examine this phenomenon further, the field in a metal cage with high electrical conductivity was measured. The static electric field inside the metal cage was also found to be reduced. An improved cage design that avoids these problems, is suggested for the study of the biologic effects of static electric fields.
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    Bioelectromagnetics 2 (1981), S. 411-413 
    ISSN: 0197-8462
    Keywords: calcium efflux ; complex permittivity ; amplitude windows ; concentric spheres ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Presented here are the numerical relationships between incident power densities that produce the same average electric field intensity within a chick brain half immersed in buffered saline solution and exposed to a uniform electromagnetic field at carrier frequencies of 50, 147, and 450 MHz. Calculations are based on modeling the buffer solution as a spherical shell in air with an inner concentric sphere of brain tissue. The results support our earlier conclusion that calcium efflux results obtained at different carrier frequencies are in agreement when related by the electric field within the brain.
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    Bioelectromagnetics 3 (1982), S. 1-2 
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    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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    Bioelectromagnetics 3 (1982), S. 17-24 
    ISSN: 0197-8462
    Keywords: dielectric methods ; permittivity ; water ; bound water ; water of hydration ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Three independent dielectric methods for the measurement of water of hydration (bound water) in a biological material are described and discussed comparatively. For well-defined aqueous solutions of biological molecules, hydration can be obtained from direct observations made on the δ dispersion or from measurement of the dielectric values of the β dispersion. For whole tissue, however, neither of these two methods is applicable, and to deduce the hydration, it is necessary to use the third technique in which the volume of the hydrated biological particle is obtained by measuring the effect of it on the known dielectric properties of pure water. The hydration can then be calculated by deducting the volume of the anhydrous particle from the experimentally determined volume of the hydrated particle. Owing to possible systemmatic errors the uncertainty in the absolute hydration value associated with this technique is rather larger than that obtained with the other two dielectric methods. For studying the differences between hydration in similar tissues, however, this objection disappears.
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    Bioelectromagnetics 3 (1982) 
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    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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    Bioelectromagnetics 3 (1982), S. 91-103 
    ISSN: 0197-8462
    Keywords: electromagnetic fields ; microwaves ; physiology ; neuroendocrine ; physiologic integration ; hyperthermia ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Electromagnetic fields have been demonstrated to elicit thermoregulatory responses, neuroen-docrine, neurochemical modulations, and behavioral reactions. These physiologic regulatory processes are exquisitely tuned, interrelated functions that constitute sensitive indicators of organismic responses to radiofrequency energy absorption (the radio frequency portion of the electromagnetic spectrum includes as one part microwaves). Assessment of the integration and correlation of these functions relative to the thermal inputs and homeokinetic reactions of the individual subjected to radiofrequency energy should permit differentiation between potential hazards that might compromise the individual's ability to maintain normal physiologic function and effects that are compensated by physiologic redundancy.
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    Bioelectromagnetics 3 (1982), S. 105-116 
    ISSN: 0197-8462
    Keywords: escape ; microwaves ; rats ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: A total of 16 female hooded rats was first observed for baseline behaviors and then they received 25 2-min trials of training, five trials per day, under one of four stimulus conditions (all ns = 4): exposure to a highly intense 918-MHz field (dose rate, 60 mW/g); exposure to photic stimulation (≈350 Ix); exposure to the field in synchrony with photic stimulation; or exposure to faradic shock (≈800 μA rms). During conditioning trials, which were separated by 2-min intertrial intervals, entry by a rat into a safe area of a multimode cavity resulted in immediate and complete cessation of stimulation; exit, in resumption. Acquisition of the escape response was rapid and highly efficient for shocked animals and was less rapid and efficient but was reliably demonstrated by irradiated animals that were also signaled by light. In the absence of microwave irradiation, cessation of light did not reliably motivate escape behavior. Although there was weak evidence of escape learning by rats subjected only to microwave irradiation, their performances failed to differ reliably from those of rats in the light-only condition. These data confirm and extend those of Carroll et al, which indicate that potentially lethal, deeply penetrating, nonpulsed microwaves in a multipath field lack the sensory quality to motivate efficient aversive behavior by the rat.
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    Bioelectromagnetics 3 (1982), S. 147-156 
    ISSN: 0197-8462
    Keywords: pulsed ultrasound ; biological ultrasound ; Drosophilia ; Elodea ; cavitation ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Studies with both plant and animal tissues show that, when the tissues contain micron-sized, stabilized gas bodies, pulsed ultrasound can produce damage at very low time-averaged intensities. However, it is the temporal peak intensity rather than the time-averaged intensity that is closely correlated with the effects observed. The data suggest that there may be thresholds for damage at peak intensities within an order of magnitude of 10 W/cm2.
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    Bioelectromagnetics 3 (1982), S. 157-165 
    ISSN: 0197-8462
    Keywords: ultrasonic absorption ; proteins ; polypeptides ; peptides ; amino acids ; tissues ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: This article reviews significant advances in understanding the basis for the magnitude of ultrasonic absorption in proteins and related biological materials. Carstensen and Schwan's accurate and extensive measurements on blood and hemoglobin solutions provided the initial experimental data; these were augmented by data from measurements on aqueous solutions of gelatin, bovine serum albumin, lysozyme, various polypeptides, and amino acids. The initial frequency range of 1-10 MHz; was expanded to 0.035-1000 MHz; temperature and pH dependences of absorption were studied. Theoretical approaches included consideration of the relative motion of blood cells in plasma, perturbation of water structure around macromolecules, solvation of charged entities, proton-transfer reactions, and helix-coil transitions.Proton-transfer reactions between amino and carboxylic groups and water proved to be largely responsible for the observed peaks in the pH dependence of absorption coefficient; the peaks occurred in the basic and acidic regions corresponding to the pKs for titration of these groups. Such reactions could not account for the magnitude of absorption at physiological pH because only histidine titrated in this range. Extensive analysis, using relaxation theory, and, measurements have shown that the proton transfer reaction between the imidazole group of histidine and hydrogen phosphate ion (in solution) provides sufficient volume change for significant ultrasonic absorption at physiological pH. Excellent agreement between theory and experiment was found with the peptide bacitracin in phosphate buffer solutions. By generalizing these results to the case of a protein, Slutsky et al estimated maximum values of frequency-dependent absorption coefficients for “typical tissue” and found them to be correct to order of magnitude, even exceeding observed values in soft tissues in some instances, instead of being far too small as was always the case in the past. Thus, in principle, adjustment of parameters, such as pK values, could bring theory and experiment into agreement for the first time.
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  • 57
    ISSN: 0197-8462
    Keywords: 2450-MHz microwaves ; cocarcinogenesis ; environmental and occupational carcinogenesis ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: C3H/HeA mice with high incidence of spontaneous breast cancer and Balb/c mice treated with 3,4-benzopyrene (BP) (by painting of the skin resulting in the development of skin cancer) were irradiated with 2,450-MHz microwaves (MW) in an anechoic chamber at 5 or 15 mW/cm2 (2 h daily, 6 sessions per week). C3H/HeA mice were irradiated from the 6th week of life, up to the 12th month of life. Balb/c mice treated with BP were irradiated either prior to (over 1 or 3 months) or simultaneously with BP treatment (over 5 months). The appearance of palpable tumors in C3H/HeA mice and of skin cancer in BP-treated Balb/c mice was checked every 2 weeks for 12 months. Two additional groups of mice were exposed to chronic stress caused by confinement or to sham-irradiation in an anechoic chamber; these served as controls. Irradiation with MWs at either 5 or 15 mW/cm2 for 3 months resulted in a significant lowering of natural antineoplastic resistance (mean number of lung neoplastic colonies was 2.8 ± 1.6 (SD) in controls, 6.1 ± 1.8 in mice exposed at 5 mW/cm2 and 10.8 ± 2.1 in those irradiated at 15 mW/cm2) and acceleration of development of BP-induced skin cancer (285 days in controls, 230 days for 5 mW/cm2 and 160 days for 15 mW/cm2). Microwave-exposed C3H/HeA mice developed breast tumors earlier than controls (322 days in controls, 261 days for 5 mW/cm2 and 219 days for 15 mW/cm2). A similar acceleration was observed in the development of BP-induced skin cancer in mice exposed simultaneously to BP and MWs (285 days in controls, 220 day for 5 mW/cm2 and 121 days for 15 mW/cm2). The acceleration of cancer development in all tested systems and lowering of natural antineoplastic resistance was similar in mice exposed to MW at 5 mW/cm2 or to chronic stress caused by confinement but differed significantly from the data obtained on animals exposed at 15 mW/cm2, where local thermal effects (“hot” spots) were possible.
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    Bioelectromagnetics 3 (1982), S. 227-235 
    ISSN: 0197-8462
    Keywords: microwaves ; pulsed-wave ; continuous-wave ; operant behavior ; DRL schedule ; rats ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: The effects of pulsed-(PW) and continuous-wave (CW) 2.8-GHz microwaves were compared on the performance of rodents maintained by a temporally defined schedule of positive reinforcement. The schedule involved food-pellet reinforcement of behavior according to a differential-reinforcement-of-low-rate (DRL) contingency. The rats were independently exposed to PW and to CW fields at power densities ranging from 1 to 15 mW/cm2. Alterations of normal performance were more pronounced after a 30-minute exposure to the PW field than to the CW field. The rate of emission of appropriately timed responses declined after exposure to PW at 10 and 15 mW/cm2, whereas exposure at the same power levels to the CW field did not consistently affect the rate of responding. Change in performance associated with microwave exposure was not necessarily related to a general decline in responding: in some instances, increases in overall rates of responding were observed.
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    Bioelectromagnetics 3 (1982), S. 253-274 
    ISSN: 0197-8462
    Keywords: radiofrequency ; nearfield ; hyperthermia ; dosimetry ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: A homogeneous, lossy circular cylinder is used as a simple model of a biological object in which interior heating is produced by the absorption of electromagnetic waves. For this model, we determined the optimum frequency, polarization, orientation and shape of applicators. Analytical and numerical results are given for both electric and magnetic line sources, with three different polarizations relative to the cylinder. Coupling efficiencies and contour plots are presented for a range of parameters. One particularly interesting result is the production of maximum energy deposition at the center of a cylinder of muscle tissue when exposed in the 100-MHz frequency range by the use of four applicators surrounding the cylinder.
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    Bioelectromagnetics 3 (1982), S. 293-293 
    ISSN: 0197-8462
    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
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    Bioelectromagnetics 3 (1982), S. 285-291 
    ISSN: 0197-8462
    Keywords: mice ; microwaves ; body mass ; development ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Time-bred CD-1 mice (100) were sham-irradiated or irradiated with 2450-MHz (CW) microwaves at 28 mW/cm2 for 100 minutes daily from the 6th through 17th day of gestation. The offspring were examined either as fetuses after hysterotomy on the 18th day of gestation or as naturally born neonates on the 1st and 7th day of age. Fetuses of half of the dams were examined on the 18th day of gestation. The incidence of pregnancy and the numbers of live, dead, resorbed, and total fetuses were similar in both groups. The mean weight was significantly lower (10%) in live microwave-irradiated fetuses, and ossification of sternal centers was significantly delayed. In the offspring that were born naturally, the mean weight of microwave-irradiated 7-day-old suckling mice was significantly lower (10%) than that of the sham-irradiated group. Survival rates of neonates in these two groups were not different. These data demonstrate that the decreased fetal weight seen in microwave-irradiated mice is retained at least 7 days after birth. Evidence from other published studies is presented to show that the retarded growth is persistent and might be interpreted as permanent stunting.
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    Bioelectromagnetics 3 (1982), S. 333-339 
    ISSN: 0197-8462
    Keywords: electromagnetic dosimetry ; man and animal models ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: A surface integral equation (SIE) method is used to calculate the specific absorption rate (SAR) in spherically capped cylindrical models irradiated by an axially incident electromagnetic plane wave (K polarization) in a frequency range for which calculations previously have not been available (80-400 MHz for man models). In the SIE method, the electromagnetic (EM) field relations are formulated in terms of electric and magnetic currents on the surface of the model. The average SAR is calculated from the far scattered EM fields by means of the forward scattering theorem. SAR data calculated by the SIE method agree with data calculated by the extended boundary condition method (EBCM) for frequencies up to 80 MHz (the upper frequency limit of the EBCM) for man models. For rat models exposed to 1-3 GHz radiation, reasonable agreement was also obtained with the limited experimental data available.
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    Bioelectromagnetics 3 (1982), S. 349-361 
    ISSN: 0197-8462
    Keywords: hemoglobin ; influence of radiation on oxygen affinity ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: When oxygen binds to one of the subunits of hemoglobin, the oxygen affinity of the other subunits is enhanced. This cooperative interaction of the subunits is initiated by the movement of the heme plane toward the proximal side when oxygen binds to the heme. This motion is transmitted to the surface of the globin through a “reaction channel” consisting of a group of atoms whose motion is well correlated. Considering the detailed geometry and X-ray diffraction data of the mean square displacement of the atoms surrounding the heme, a simple model for the heme plane oscillations is developed. Using this model, the natural frequency of oscillations is shown to be ≈5 × 1011 Hz. This result, along with the recent experimental data on the kinetics of the conformational changes of the heme, points to the possibility of radiation influencing the oxygen affinity of hemoglobin. If such an effect exists, it is likely that the oxygen affinity will be enhanced by the radiation.
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    Bioelectromagnetics 3 (1982), S. 363-370 
    ISSN: 0197-8462
    Keywords: microwave ; hyperthermia ; hamster ; PARA-7 tumor ; computer ; 2.45 GHz ; temperature distribution ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: A minicomputer-based system was designed to control the microwave (2.45-GHz) power to four local hyperthermia applicators. Errors in temperature measurement, due to electromagnetic field interactions with small thermocouple probes, are minimized by sampling the temperature only when the microwave power is off. The programmable controller can regulate the temperature in tumors in 0.1 °C increments from 30 to 60 °C. This technique reduces temperature differences throughout the tumor at steady state to less than 0.4 °C and prevents skin burns.
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    Bioelectromagnetics 3 (1982) 
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    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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  • 66
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    Bioelectromagnetics 3 (1982), S. 371-383 
    ISSN: 0197-8462
    Keywords: blood-brain barrier ; rats ; 2450-MHz microwaves ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Adult rats anesthesized with pentobarbital and injected intravenously with a mixture of [14C]sucrose and [3H]inulin were exposed for 30 min to an environment at an ambient temperature of 22, 30, or 40 °C, or were exposed at 22 °C to 2450-MHz CW microwave radiation at power densities of 0, 10, 20, or 30 mW/cm2. Following exposure, the brain was perfused and sectioned into eight regions, and the radioactivity in each region was counted. The data were analyzed by two methods. First, the data for each of the eight regions and for each of the two radioactive tracers were analyzed by regression analysis for a total of 16 analyses and Bonferroni's Inequality was applied to prevent false positive results from numerous analyses. By this conservative test, no statistically significant increase in permeation was found for either tracer in any brain region of rats exposed to microwaves. Second, a profile analysis was used to test for a general change in tracer uptake across all brain regions. Using this statistical method, a significant increase in permeation was found for sucrose but not for inulin. A correction factor was then derived from the warm-air experiments to correct for the increase in permeation of the brain associated with change in body temperature. This correction factor was applied to the data for the irradiated animals. After correcting the data for thermal effects of the microwave radiation, no significant increase in permeation was found.
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    Bioelectromagnetics 3 (1982), S. 401-412 
    ISSN: 0197-8462
    Keywords: 2,450 MHz ; exposure system ; circular waveguide ; anechoic chamber ; mice ; dosimetry ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Two systems for exposing mice to 2,450-MHz electromagnetic fields are described. In a waveguide system, four mice were placed in a Styrofoam cage and exposed dorsally to circularly polarized electromagnetic fields. The temperature and humidity in the mouse holder were kept constant by forced-air ventilation. For 1-W input power to the waveguide, the average specific absorption rate (SAR) was determined by twin-well calorimetry to be 3.60 ± 0.11 (SE) W/kg in 27-g mice. The maximum SAR at the skin surface determined thermographically was 8.36 W/kg in the head of the mouse. The second system was a miniature anechoic chamber. Six mice were irradiated dorsally to far field plane waves. Copper shielding and high-temperature absorbing material were lined inside the chamber to accommodate the high input power. The air ventilation at the location of the mice was separately controlled so that any heating in the absorber would not affect the animals. For 1-W input power, the average SAR was 0.17 ± 0.01 W/kg and the maximum SAR at the skin surface was 0.41 W/kg in the animal when irradiated with body axis parallel to the E field; the SARs were 0.11 ± 0.01 W/kg and 0.64 W/kg, respectively, when irradiated perpendicular to the E field.
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    Bioelectromagnetics 3 (1982), S. 443-451 
    ISSN: 0197-8462
    Keywords: miniature swine ; ELF ; 60-Hz electric field ; behavior ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: It has been shown that rats, given the choice, will spend more time out of a 60-Hz electric field than in it at field strengths ≥ 75 kV/m. This paper describes research to examine the relevance of these data to a different species, the pig. Miniature pigs that had been exposed to a 60-Hz electric field at 30 kV/m for 20 h/day, 7 days/week for as long as 6 months, were tested for their preference for the presence or absence of the field during a 23.5-h period. Similar to earlier results with rats, miniature pigs spent more time out of the electric field than in it during the sleeping period.
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    Bioelectromagnetics 3 (1982), S. 471-474 
    ISSN: 0197-8462
    Keywords: 8.8 GHz ; pulsed microwave ; thermal vs microwave alteration ; E coli ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Escherichia coli pol A+ and pol A- strains were exposed to 8.8-GHz microwaves pulsed at 1,000 Hz (1-μs pulse width) and an SAR of 40 W/kg, which increased the temperature of the cell culture by 7 °C. Two-way analysis of variance showed no significant difference between the growth rates of microwave irradiated and thermally exposed cells.
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  • 70
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    Bioelectromagnetics 4 (1983) 
    ISSN: 0197-8462
    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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    Bioelectromagnetics 4 (1983), S. 11-19 
    ISSN: 0197-8462
    Keywords: electric field ; bone growth ; osteotomy repair ; rats ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Rats were exposed to a 60-Hz electric field at an unperturbed field strength of 100 kV/m to determine its affect on bone growth and fracture repair. Exposure of immature male and female rats for 20 h/day for 30 days did not alter growth rate, cortical bone area, or medullary cavity area of the tibia. In another experiment, midfibular osteotomies were performed and the juvenile rats were exposed at 100 kV/m for 14 days. Evaluation by resistance to deformation and breaking strength indicated that fracture repair was not as advanced in the exposed animals as in the shamexposed animals. In another experiment measurements of resistance to deformation were made in adult rats at 16, 20, and 26 days after osteotmy. Fracture repair was slower in exposed compared to control animals at day 20 and, to a lesser extent, at day 16, but not at day 26.
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    Bioelectromagnetics 4 (1983), S. 55-62 
    ISSN: 0197-8462
    Keywords: 2880-MHz microwaves ; submaxillary salivary gland ; Na+ ; K+ ; Ca2+ ; flame photometry ; hyperthermia ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Na+, K+, and Ca2+ concentrations in the blood serum and submaxillary salivary gland (SSG) were investigated in adult, male rats exposed to 2880-MHz microwaves modulated with 1.5-μs pulses at a pulse repetition rate of 1000 Hz or in a hyperthermal environment. Rats were exposed, one at a time, for 30 min to microwaves producing a specific absorption rate (SAR) of: 4.2, 6.3,6.8,8.4, 10.8, or 12.6 W/kg, or were sham exposed under similar environmental conditions. In a second series, one group of rats was exposed singly for 15, 30, or 60 min to microwaves producing an SAR of 9.5 W/kg and other rats were exposed for similar periods at 40 °C; and 10 rats were sham exposed. Flame photometric analysis indicated that the thresholds of microwave radiation required to induce a change in Na+, K+, and Ca2+ concentrations in the salivary glands are 6.8, 6.8, and 6.3 W/kg, respectively. The directions of Na+, K+, and Ca2+ ion shifts in exposed rats' salivary glands are similar, whether affected by microwaves or hyperthermia. Greater changes in Na+ and K+ concentrations in SSG of rats exposed to microwaves for 15 and 30 min were found than in those exposed at 40 °C. On the other hand, exposure to hyperthermia at 40 °C or to microwaves for 1 h caused Na+ concentration to be increased by 68.7 and 59.5% and K+ concentration to be decreased by 29.6 and 21.7%, respectively.
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    Bioelectromagnetics 4 (1983), S. 63-77 
    ISSN: 0197-8462
    Keywords: microwaves ; exposure chamber ; dosimetry ; rabbit ; body mass ; food consumption ; blood chemistry ; pathology ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Two groups of 16 male New Zealand rabbits were exposed to 2450-MHz continuous wave microwave fields in two experiments of 90 days each. The incident power densities of the first and second experiment were 0.5 and 5 mW/cm2, respectively. During each study, 16 animals were adapted to a miniature anechoic chamber exposure system for at least 2 weeks, then 8 of them were exposed for 7 h daily, 5 days a week for 13 weeks, and the other 8 animals were sham exposed. The rabbits were placed in acrylic cages, and each was exposed from the top in an individual miniature anechoic chamber. Thermography showed a maximum specific absorption rate of 5.5 W/kg in the head and 7 W/kg in the back at 5-mW/cm2 incident power density. After each 7-h exposure session, the animals were returned to their home cages. Food consumption in the exposure chamber and body mass were measured daily. Blood samples were taken before exposure and monthly thereafter for hematological, morphological, chemical, protein electrophoresis, and lymphocyte blast transformation studies. Eyes were examined for cataract formation. Finally, pathological examinations of 28 specimens of organs and tissues of each rabbit were performed. Statistically, there was a significant (P 〈 .01) decrease only of food consumption during the 5-mW/cm2 exposure; other variables were not significantly different between exposed and control groups.
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    Bioelectromagnetics 4 (1983), S. 107-114 
    ISSN: 0197-8462
    Keywords: spermatogenesis ; microwave radiation ; germinal tissue ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Male Sprague-Dawley rats were exposed for 6 h per day for nine days to pulse-modulated microwave radiation (1.3 GHz, at 1-μs pulse width, 600 pulses per second). Exposures were carried out in cylindrical waveguide sections at a mean dose rate of 6.3 mW/g; sham controls were treated similarly and received no irradiation. At time periods corresponding to 0.5, 1.0, 2.0, and 4.0 cycles of the seminiferous epithelium, groups of four shamirradiated and four irradiated rats were killed and the testes removed for analysis. Net mass of the testes, epididymides, and seminal vesicles; daily sperm production (DSP) per testis and per gram of testis; sperm morphology; and the number of epididymal sperm were determined. There were no statistically significant differences between the shamirradiated and irradiated groups with respect to any measured variable. In a group of seven surrogate animals of similar body mass, the dose rate of 6.3 mW/g caused a net change in body temperature (via rectal probe) of 1.5 °C.
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    Bioelectromagnetics 4 (1983), S. 141-155 
    ISSN: 0197-8462
    Keywords: microwave bioeffects ; hamster macrophages ; immunology ; viricidal macrophages ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Acute exposure of hamsters to microwave energy (2.45 GHz; 25 mW/cm2 for 60 min) resulted in activation of peritoneal macrophages that were significantly more viricidal to vaccinia virus as compared to sham-exposed or normal (minimum-handling) controls. Macrophages from microwave-exposed hamsters became activated as early as 6 h after exposure and remained activated for up to 12 days. The activation of macrophages by microwave exposure paralleled the macrophage activation after vaccinia virus immunization. Activated macrophages from vacciniaimmunized hamsters did not differ in their viricidal activity when the hamsters were microwave or sham-exposed. Exposure for 60 min at 15 mW/cm2 did not activate the macrophages while 40 mW/cm2 exposure was harmful to some hamsters. Average maximum core temperatures in the exposed (25 mW/cm2) and sham groups were 40.5 °C (±0.35 SD) and 38.4 °C (±0.5 SD), respectively. In vitro heating of macrophages to 40.5 °C was not as effective as in vivo microwave exposure in activating macrophages to the viricidal state. Macrophages from normal, shamexposed, and microwave-exposed hamsters were not morphologically different, and they all phagocytosed India ink particles. Moreover, immune macrophage cytotoxicity for virus-infected or noninfected target cells was not suppressed in the microwave-irradiated group (25 mW/cm2, 1 h) as compared to sham-exposed controls, indicating that peritoneal macrophages were not functionally suppressed or injured by microwave hyperthermia.
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    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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    Bioelectromagnetics 4 (1983), S. 215-247 
    ISSN: 0197-8462
    Keywords: electric field ; 60-Hz ; detection ; psychophysics ; rats ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Rats partially deprived of food were trained individually to press a lever in the presence of a vertical, 60-Hz electric field and not to press in its absence. Correct detections that occurred during brief, 3- or 4-s trials occasionally produced a food pellet. The probability of detecting the field was found to increase as field strength increased. The threshold of detection, ie, the field strength required for detections at a probability of 0.5 after correction for errors, was generally between 4 and 10 kV/m. The range of field strengths between almost zero and almost 100% correctness of detection was approximately 8 kV/m. A logistic function provided a good description of the increase in the detection probability with increasing field strength. These performances occurred reliably in 19 rats, some of which were studied for 2 years. Control procedures showed that the behavior required that the rat be in the electric field; the behavior was not controlled by any of several potentially confounding variables.
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    Bioelectromagnetics 4 (1983), S. 257-265 
    ISSN: 0197-8462
    Keywords: shortwave ; diathermy applicators ; heating ; attenuation ; conductivity ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Tissue-substitute models consisting of layers of synthetic, electrically equivalent subcutaneous fat, muscle, and bone shaped in conformation with the normal anatomy are used for rapid determination of distribution of temperature and specific absorption rate throughout the tissues when exposed to electromagnetic radiation. The surfaces of the bisected models are approximated during a short exposure period, then separated and scanned with a thermograph. A method was developed to eliminate the electrical discontinuity at the bisected surfaces while allowing separation and subsequent thermographic scanning. A thin layer of silk screen wetted with propylene glycol saturated with sodium chloride was used at the fat interface and a 0.9% sodium chloride solution was used to wet the screen at the muscle interface to eliminate electrical discontinuity during exposure to 27.12-MHz diathermy. Tests showed that in the presence of an electrical discontinuity the heating pattern was grossly distorted. With the method used, the electrical discontinuity is minimized and the subsequent thermographic scanning reveals that the heating pattern is equivalent to that of an intact model.
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    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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    Bioelectromagnetics 4 (1983), S. 315-326 
    ISSN: 0197-8462
    Keywords: Drosophila ; ELF electromagnetic effects ; oviposition ; development ; viability ; magnetic fields ; DC magnetic fields ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Drosophila flies placed in a habitat with two lateral boxes demonstrated sensitivity to magnetic fields: Oviposition decreased by exposure to pulsated extremely low frequency (ELF) (100)Hz, 1.76 miliTesla (mT) and sinusosidal fields (50 Hz, 1 mT), while there was no initial effect of exposure to a static magnetic field (4.5 mT). Drosophila eggs treated for 48 h with the above described fields showed that (1) mortality of eggs was lower in controls than in eggs exposed to all tested magnetic fields; (2) mortality of larvae increased when a permanent magnet was used; (3) mortality of pupae was highest when a permanent magnet was used; and (4) general adult viability was highest in controls (67%) and diminished progressively when eggs were exposed to pulsated (55%), sinusoidal (45%), and static (35%) magnetic fields.
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    Bioelectromagnetics 4 (1983), S. 357-370 
    ISSN: 0197-8462
    Keywords: Helmholtz coils ; electromagnetic fields ; induced currents ; saline media ; biological tissue ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: The use of Helmholtz aiding coils to induce small electromagnetic (EM) fields in living tissue for both medical and research purposes has become quite common. While much progress has been made in showing that these induced EM signals can cause a variety of effects in tissues and individual cells, a satisfactory explanation of how the effects occur or how the EM signals couple to the tissue has not yet emerged. To address the latter problem adequately, it becomes necessary to know the spatial distribution of the induced fields inside a given set of boundaries. This paper examines the situation used for much in vitro research where a cylindrical culture dish is filled with a conducting solution and placed between the Helmholtz coils. Two cases are considered. The first assumes that the coils are above and below the culture dish (the planes of the coils are parallel with the top and bottom of the dish); the second assumes that the planes of the coils are parallel with the sides of the dish. A closed form solution is obtained for both cases, and it is shown that the induced EM field distribution is markedly different for the two cases.
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    Bioelectromagnetics 4 (1983), S. 397-400 
    ISSN: 0197-8462
    Keywords: acoustical imaging ; microwave-induced acoustics ; pulsed microwaves ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Pulsed 5.66-GHz microwave energy irradiated a model of a human hand that was positioned above a submerged planar array of 400 hydrophones. Hydrophone response data were analyzed by a computer that graphically reproduced the image.
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    Bioelectromagnetics 5 (1984), S. 31-38 
    ISSN: 0197-8462
    Keywords: Characean cells ; vacuolar potential ; electromagnetic radiation ; Nitella flexilis ; Chara braunii ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Measurements were made of the small, transient offsets of vacuolar potential produced in single cells of Nitella flexilis and Chara braunii by isolated bursts of audio frequency electromagnetic radiation. The offsets increased in magnitude with decreasing frequency of the electromagnetic radiation and, below about 6 kHz, seemed to approach a lowfrequency asymptote. This frequency dependence for the offset is shown to be in accordance with a previously developed model in which the incident radiation is weakly rectified by the cell's membrane system.
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    Bioelectromagnetics 5 (1984), S. 47-62 
    ISSN: 0197-8462
    Keywords: digital computer simulation ; alternating electrical field ; electromagnetic field ; biological sturcture ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: A digital computer simulation has been carried out of the exposure of a cell, modeled as a multilayered spherical structure, to an alternating electrical field. Electrical and electrochemical quantities of possible biological interest can be evaluated everywhere inside the cell. A strong frequency behavior in the range 0-10 MHz has been obtained.
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  • 85
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    Bioelectromagnetics 5 (1984), S. 79-88 
    ISSN: 0197-8462
    Keywords: circular waveguide exposure system ; radiofrequency dosimetry ; rhesus monkey ; radiofrequency exposure ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: A 275-MHz exposure system, consisting of a circular waveguide irradiator and a transparent plastic animal cage, has been developed to accommodate rhesus monkeys weighing up to 15 kg. The vertically oriented waveguide is composed primarily of stainless steel and is fitted with an inner cage fabricated from a tubular section of acrylic plastic. Circularly polarized electromagnetic energy at 275 MHz, either pulsed or continuous wave (CW), can be propagated from the removable top section of the waveguide. The cage is designed to function as the monkey's permanent home. It is fitted with a lever-actuated behavioral performance device on which the monkey responds according to a predetermined schedule to obtain a daily food ration. The system can be adapted to provide for the collection of metabolic and physiologic data as well. Dosimetric measurements were conducted with six rhesus monkeys weighing 3.0-7.2 kg and with a 4-kg model. The dosimetric results show that about one-third of the net incident energy is absorbed by a subject in this system at a normalized specific absorption rate (SAR) of 0.33 (W/kg)/(mW/cm2).
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    Bioelectromagnetics 5 (1984), S. 89-99 
    ISSN: 0197-8462
    Keywords: specific absorption rate ; resonant cavity ; spheres ; Mie theory ; superposition ; thermography ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Specific absorption rates (SARs) were determined theoretically and experimentally for several spherical models of tissue exposed to electrical fields of TE101 mode in a rectangular cavity of 57.3 MHz resonant frequency. The approximate theoretical SAR can be calculated according to the Mie theory by superposition of four plane waves representing the fields excited in the cavity. The theoretical and thermographically determined SAR patterns in spheres with radii of 5, 7.5, and 10 cm and with conductivities of 0.1, 1, and 10 S/m were compared. For a sphere with radius less than 7.5 cm and conductivity less than 1 S/m, the SAR was quite uniform. When conductivity was increased to 10 S/m, the SAR patterns showed higher absorption in the periphery of the largest sphere (10-cm radius). These characteristics are important in evaluating the scaling technique of exposing a model of a human to very-high-frequency fields to obtain power absorption data in humans exposed to high-frequency or very-low-frequency fields.
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    Bioelectromagnetics 5 (1984), S. 113-115 
    ISSN: 0197-8462
    Keywords: Nernst equation ; temperature derivatives ; cell membranes ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: A hyperpolarized current is predicted from the Nernst equation for conditions of positive temperature derivatives with respect to time. This ion current, coupled with changes in membrane channel conductivities, is expected to contribute to a transient potential shift across the cell membrane for silent cells and to a change in firing rate for pacemaker cells.
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    Bioelectromagnetics 5 (1984) 
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    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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  • 89
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    Bioelectromagnetics 5 (1984), S. 131-146 
    ISSN: 0197-8462
    Keywords: human ; dosimetry ; safety ; radio frequency ; high frequency ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: The radiofrequency absorption rates of five male human volunteers have been measured from 3 to 41 MHz. The subjects were exposed at about 10 μW/cm2 inside a very large transverse electromagnetic (TEM) cell and never absorbed more than 1 W. Both the EKH and EHK orientations were employed under both free-space and grounded conditions. Absorption rates for the EKH orientation exceed those of the EHK orientation by 40% in free space, but only by 6% when grounded. The absorption rates for the grounded men vary with frequency, f, as f1.9 from 3 to 25 MHz and then level off at the peak. The freespace absorption rates vary as f1.7 from 3 to 18 MHz and as f2.9 from 18 to 41 MHz. The average measured absorption rates at 10 MHz exceed the average of the standard model calculations by a factor of three (for free space) or four (grounded). The average man, when exposed grounded in an EKH orientation to the maximum permitted exposure levels under ANSI standard C95.1-1982, will absorb 0.58 ± 0.14 W/kg over most of the 3 to 41-MHz frequency range. This slightly exceeds the whole-body maximum of 0.40 W/kg underlying the standard.
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    Bioelectromagnetics 5 (1984), S. 193-202 
    ISSN: 0197-8462
    Keywords: bioelectricity ; bone ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: A high coefficient of variation is characteristic of the bioelectric potentials recorded from living long bones. As a consequence, the data collected from animal and human experiments are difficult to use in practical ways. A distribution curve for bone bioelectric potentials has been calculated using polynomial regression analysis to process the voltage values recorded on the whole length of rabbit tibiae, with reference electrodes positioned in three different points of the bone. The distribution curve so obtained is presented to fulfill the need for a reference curve for the bioelectric potentials recorded from rabbit tibial surfaces.
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    Bioelectromagnetics 5 (1984), S. 263-270 
    ISSN: 0197-8462
    Keywords: frog ; heart rate ; microwaves ; electrodes ; bradycardia ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: One hundred and two isolated frog hearts were divided into ten groups and placed individually in a waveguide filled with Ringer's solution and exposed to 2,450-MHz CW radiation at 2 and 8.55 W/kg. Heart rate was recorded using one of the following methods: 3-M KCl glass electrode, ultrasound probe, tension transducer, Ringer's solution glass electrode, and a metal wire inserted in the Ringer's solution electrode. An accelerated decrease of heart rate was observed only in those groups recorded using the 3-M KCl electrode and the metal wire Ringer's solution electrode. No effect was found in the other groups. These results indicate that bradycardia in isolated hearts could be caused by electrode artifacts resulting from the intensification of electromagnetic fields.
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    Bioelectromagnetics 5 (1984), S. 233-246 
    ISSN: 0197-8462
    Keywords: operant behavior ; observing-responses ; radiofrequency radiation ; colonic temperature ; rhesus monkeys ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Five food-deprived rhesus monkeys were exposed to 225-MHz continuous-wave, and 1.3-GHz, and 5.8-GHz pulsed radiation to determine the minimal power densities affecting performance. The monkeys were trained to press a lever (observing-response) thereby producing signals that indicated availability of food. In the presence of the aperiodically appearing food signals, a detection response on a different lever was reinforced by a food pellet. Continuous, stable responding during 60-min sessions developed and was followed by repeated exposures to radiofrequency radiation. The subjects, restrained in a Styrofoam chair, were exposed to free-field radiation while performing the task. Colonic temperature was simultaneously obtained. Observing-response performance was impaired at increasingly higher power densities as frequency increased from the near-resonance 225 MHz to the above-resonance 5.8 GHz. The threshold power density of disrupted response rate at 225 MHz was 8.1 mW/cm2; at 1.3 GHz it was 57 mW/cm2, and at 5.8 GHz it was 140 mW/cm2. These power densities were associated with reliable increases in colonic temperatures above sham-exposure levels. The mean increase was typically in the range of 1°C, and response-rate changes were not observed in the absence of concomitant temperature increases. In these experiments increase of colonic temperature was a much better predictor of behavioral disruption than was either the power density of the incident field or estimates of whole-body-averaged rates of energy absorption.
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    Bioelectromagnetics 5 (1984), S. 271-282 
    ISSN: 0197-8462
    Keywords: Electric fields ; 60 Hz ; rats ; behavior ; gastrointestinal distress ; taste aversion ; behavior toxicolgy ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: A measure of taste-aversion (TA) learning was used in three experiments to 1) determine whether exposure to intense 60-Hz electric fields can produce TA learning in male Sprague-Dawley rats, and 2) establish a dose-response function for the behavior in question. In Experiment 1, four groups of eight rats each were distributed into one of two exposures (69 ± 5 kV/m or 133 ± 10 kV/m) or into one of two sham-exposure groups. Conditioning trials paired 0.1% sodium saccharin in water with 3 h of exposure to a 60-Hz electric field. Following five conditioning trials, a 20-min, two-bottle preference test between water and saccharin-flavored water failed to reveal TA conditioning in exposed groups. In Experiment 2, four groups of eight rats each (34 ± 2 kV/m or 133 ± 10 kV/m and two sham-exposed groups) were treated as before. Electric-field exposure had no effect on TA learning. Experiment 3 tested for a possible synergy between a minimal dose (for TA learning) of cyclophosphamide (6 mg/kg) and 5 h of exposure to 133 ± 10 kV/m electric fields in a dark environment under conditions otherwise similar to those of Experiments 1 and 2. The results indicated no TA learning as reflected in the relative consumption of saccharin.
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    Bioelectromagnetics 5 (1984), S. 305-314 
    ISSN: 0197-8462
    Keywords: Apis mellifera ; 765-kV transmission line ; E-field ; stress ; hemocytes ; hemoproteins ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Number of circulating hemocytes and hemolymph protein patterns of adult worker honey bees were analyzed as possible indicators of stress resulting from colony placement under a 765-kV transmission line. Although exposure to 55, 80, and 95 μA total induced hive current (THC) produced colony behavioral disturbance, there were no consistent effects on mean hemocyte counts at 55- or 95-μA THC. Age-dependent declines in circulating hemocyte number were similar in all exposure groups. There were no consistent differences in tube-gel electropherograms. No consistent differences were found in two-density slab-gel electropherograms based on ultrasensitive silver stain. The 67 positively charged and four negatively charged protein fractions from overwintering bees are two- to threefold more than currently reported in the literature.
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    Bioelectromagnetics 11 (1990) 
    ISSN: 0197-8462
    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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    Bioelectromagnetics 11 (1990), S. 5-11 
    ISSN: 0197-8462
    Keywords: Life and Medical Sciences ; Occupational Health and Environmental Toxicology
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    Bioelectromagnetics 11 (1990), S. 37-45 
    ISSN: 0197-8462
    Keywords: cyclic magnetic fields ; embryogenesis ; developmental delay ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Continuous exposure of sea urchin (Strongylocentrotus purpuratus) embryos at 18°C to a cyclic 60-Hz magnetic field at 0.1 mT rms beginning 4 min after insemination caused a significant developmental delay during the subsequent 23 hours. No delay in development was recorded for periods up to 18 hours after fertilization. At 18 h, most embryos were in the mesenchyme blastula stage. At 23 h, most control embryos were in mid-gastrula whereas most magnetic-field-exposed embryos were in the early gastrula stage. Thus an estimated 1-h delay occurred between these developmental stages. The results are discussed in terms of possible magnetic-field modification of transcription as well as interference with cell migration during gastrulation. The present study extends and supports the growing body of information about potential effects of exposures to extremely-low-frequency (ELF) magnetic fields on developing organisms.
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    Bioelectromagnetics 11 (1990), S. 91-99 
    ISSN: 0197-8462
    Keywords: E fields ; H fields ; cancer ; epidemiology ; occupational ; residential ; study designs ; exposure assessment ; guidelines ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: The reported association between the risk of human cancer and exposure to 50- or 60-Hz electric and magnetic fields is difficult to evaluate from studies published to date. The association is now being reexamined in several large epidemiologic studies. In most of the studies, exposure will be assessed with newly designed, portable meters that allow direct and precise measurements of exposure to be performed easily for large numbers of individuals. The main features of the studies are summarized. At a meeting of principal investigators held in 1988 at the International Agency for Research on Cancer, broad guidelines were agreed for the design of this new generation of studies. These guidelines should improve the comparability of results and eventually provide a clearer assessment of human-cancer risk from exposure to extremely low-frequency electric and magnetic fields.
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    Bioelectromagnetics 11 (1990), S. 105-116 
    ISSN: 0197-8462
    Keywords: rats ; 60-Hz electromagnetic fields ; perinatal exposure ; conditioning ; altered operant behavior ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Rats were sham exposed or exposed perinatally to a 60-Hz electromagnetic field, 22 days in utero and the first 8 days post partum. Each of the 30 once-daily exposures was 20 h in duration. The electric component of the field was vertical 30 kV/m rms, and the magnetic field component was 100 μTG rms. Later, as adults, male rats were trained to emit an operant response when reinforced with food on a multiple, random-interval schedule. Exposed rats (N = 21) gradually came to respond at significantly lower rates than did sham-exposed controls (N = 20). This finding was confirmed and extended in a second, independently performed experiment. After a sequence including operant conditioning followed by experimental extinction of responding and then by a suspension of conditioning and finally by more than a month of reconditioning, slower rates of responding were found to persist in the adult animals. The evidence of altered behavior several months after combined, fetal-neonatal exposure to an electromagnetic field presents an interesting contrast with other findings: Field-exposed rats did not differ from sham-exposed rats in terms of body mass, physical appearance, grossly observed activity level, or incidence of disease.
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    Bioelectromagnetics 11 (1990), S. 139-147 
    ISSN: 0197-8462
    Keywords: power lines ; cancer ; electric blankets ; Life and Medical Sciences ; Occupational Health and Environmental Toxicology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Physics
    Notes: Emission data are inadequate to characterize the contribution of a source to the total personal extremely-low-frequency (ELF) magnetic field exposure. In this paper, a simple model is proposed that takes into consideration the position of the subject with respect to the source and the duration of exposure. The magnetic field is spatially averaged over the whole body of the exposed subject and integrated over time. Exposure is regarded as significant if it approaches or exceeds 400 μT-h/year. By use of this method, the ELF magnetic fields generated by several household sources were compared with the levels of residential external sources, to assess their relative significance. Some common domestic electrical appliances are found to be responsible for an exposure comparable to that from power lines. When the model is used to assess exposure to electric blankets, apparently conflicting findings may be reconciled.
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