ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Articles  (74)
  • Data
  • Colloidal gold
  • SEM
  • Springer  (38)
  • Wiley-Blackwell  (36)
  • ELSEVIER
  • Elsevier
  • Macmillian Magazines Ltd.
  • Molecular Diversity Preservation International
  • Nature Publishing Group (NPG)
  • 1990-1994  (50)
  • 1985-1989  (24)
Collection
  • Articles  (74)
  • Data
Source
Keywords
Publisher
  • Springer  (38)
  • Wiley-Blackwell  (36)
  • ELSEVIER
  • Elsevier
  • Macmillian Magazines Ltd.
    • +
Years
Year
Topic
  • 1
    Electronic Resource
    Electronic Resource
    Preparation and characterization of electrophoretically deposited high-temperature superconductor coatings (1994)
    Springer
    Microchimica acta 113 (1994), S. 19-27 
    Details
    ISSN: 1436-5073
    Keywords: high-temperature superconductors ; electrophoretic coatings ; XRD ; SQUID ; SEM ; EPMA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The high-temperature superconductor, YBa2Cu3O7−x (x = 0.1−0.2) [YBCO], was prepared using an optimized calcination and sintering process. Thin layers of a few microns of this material were deposited on a silver substrate by applying a simplified electrophoretic deposition technique in a suspension of the fine, 〈 10 μm, superconductor powder in a non-aqueous liquid. To get a uniform and strongly adherent coating, the deposition process is repeated several times, followed by an appropriate sintering procedure. The initially prepared YBCO powder and the coatings produced were characterized for their superconducting properties by X-ray diffraction analysis (XRD), magnetization measurements with a Superconducting Quantum Interference Device (SQUID) and electrical resistivity measurements. By scanning electron microscopy (SEM) and electron probe microanalysis (EPMA) the grain size of the YBCO film, its thickness and impurity content, respectively, were estimated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01243133
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Microstructural analysis of faulting in quartzite, Assynt, NW Scotland: Implications for fault zone evolution (1994)
    Springer
    Pure and applied geophysics 143 (1994), S. 229-254 
    Details
    ISSN: 1420-9136
    Keywords: Structural geology ; faults ; deformation mechanisms ; NW Scotland ; SEM
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract Macroscopic fracture arrays, microstructures and interpreted deformation mechanisms are used to assess the development of a minor reverse fault (backthrust) in quartzite from the Moine Thrust Zone, Assynt, NW Scotland. Fracturing dominates the faulting via the progression: intragranular extension microcracks; transgranular, cataclasite absent extension fractures; through-going, cataclasite filled shear microfaults, within which fracturing and particulate flow operate. However, both diffusive mass transfer (DMT) and intracrystalline plasticity (low temperature plasticity, LTP) processes also contribute to the fault zone deformation and lead to distinct associations of deformation mechanisms (e.g., DMT-fracture and LTP-fracture or low-temperature ductile fracture, LTDF). Over a large range of scales the fault zone consists of blocks of relatively intact rock separated by narrow zones of intense deformation where fracture processes dominate. The populations of fragments/blocks of different sizes in the fault zone have a power-law relationship which is related to the dimension of the fault zone. These observations are used to develop a general model for fault zone evolution based on the distribution of deformation features as a function of either time or space. A systematic variation in the deformation rate: time histories is recognised, associated with different positions within the fault zone. Thus, the fault zone preserves elements of the “birth, life and death” sequences associated with the displacement history and strain accommodation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00874330
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Morphological and molecular characterization of Frankia sp. isolates from nodules of Alnus nepalensis Don (1994)
    Springer
    Archives of microbiology 161 (1994), S. 152-155 
    Details
    ISSN: 1432-072X
    Keywords: Frankia sp. ; Alnus nepalensis ; SEM ; PCR ; Nucleotide sequence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Nodules collected from Alnus nepalensis growing in mixed forest stands at three different sites around Shillong, were crushed in various culture media to obtain isolates of Frankia. The isolates were found to have typical Frankia morphology as revealed by the scanning electron microscope. Seedlings inoculated with isolates or crushed nodules formed nitrogen fixing nodules. Frankia specific DNA probes amplified the DNA of the tested isolate AnpUS4. Partial nucleotide sequence of the 16S rRNA gene indicated that AnpUS4 was phylogenetically distinct from all other Frankia strains characterized so far.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00276476
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Nutlet sculpturing of selectedTeucrium species (Lamiaceae): A character of taxonomic significance (1994)
    Springer
    Plant systematics and evolution 192 (1994), S. 199-214 
    Details
    ISSN: 1615-6110
    Keywords: Lamiaceae ; Teucrium ; SEM ; nutlet sculpturing ; oil glands ; trichomes ; micromorphology ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Nutlet sculpturing of 23Teucrium species belonging to six sections was studied. The nutlets of analysed species were characterized by the presence of the oil glands of various density. SectionTeucrium is well defined by very densely distributed trichomes and oil glands on nutlets. Trichomes on nutlets were absent in all other species, except inT. marum from sect.Chamaedrys. Sect.Polium is a homogeneous group, distinguished from the other sections. There are some similarities of this sect. with sect.Scordium. Species of sect.Chamaedrys are heterogeneous and overlap according to the surface sculpturing characters with the other sections. The significance of nutlet microcharacters as additional taxonomical markers in the infrageneric classification of the genusTeucrium is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00986252
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Polyvinyl alcohol hydrogels I. Microscopic structure by freeze-etching and critical point drying techniques (1994)
    Springer
    Colloid & polymer science 272 (1994), S. 301-309 
    Details
    ISSN: 1435-1536
    Keywords: Hydrogels ; polyvinyl alcohol ; structures ; SEM ; freeze-etching ; critical point drying
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Froeze-etching (FE) and critical point drying (CPD) techniques were employed to prepare samples for investigating surface and bulk structures of polyvinyl alcohol (PVA) hydrogels by scanning electron microscopy. The hydrogels were obtained by freezing homogeneous solutions containing PVA polymer in either water or an aqueous solution of dimethyl sulfoxide (DMSO). An oriented porous structure was observed in the PVA hydrogel prepared without DMSO. The structure on the surface was found to be more porous than in the bulk for PVA hydrogels prepared from aqueous DMSO solutions. For given compositions of the hydrogels, samples prepared by FE technique showed a highly porous fibrillar structure on the surface, while those prepared by CPD technique showed a collapsed fibrillar structure with much less porosity. This marked difference indicates a collapse of the surface structure caused by the CPD technique. The CPD technique also led to significant reduction in porosity and loss of fibrillar structure in the bulk. Volume shrinkage of hydrogels caused by dehydration in ethanol may be responsible for the surface collapse as well as alteration of bulk structure. The FE technique reveals a more native structure of hydrogels than the commonly used CPD technique. However, it suffers from disadvantages such as charging and structural damage at high magnifications.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00655501
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Scanning electron microscopy of shoot differentiationin vitro from leaf explants of the African violet (1994)
    Springer
    Plant cell, tissue and organ culture 36 (1994), S. 157-162 
    Details
    ISSN: 1573-5044
    Keywords: epidermal cell ; Saintpaulia ionantha ; SEM
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The shoot differentiation process from leaf explants ofSaintpaulia ionantha Wendl. ‘Gypsy Trail’ culturedin vitro was investigated via scanning electron microscopy. From 16 combinations of α-naphthaleneacetic acid (NAA) and 6-benzyladenine (BA), the optimum concentration for direct shoot formation without callus formation for the cultivar tested was estimated as 0.5 μM NAA and 0.5μM BA. The first cell divisions were observed after 5 days, in culture and were restricted to cells adjacent to the basal cells of glandular hairs. Meristematic domes were formed after 15 days and were investigated at 20 days. The origin of shoot formation was restricted to epidermal cells adjacent to basal cells of glandular hairs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00037714
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Gill epithelial cells kinetics in a freshwater teleost, Oncorhynchus mykiss during adaptation to ion-poor water and hormonal treatments (1994)
    Springer
    Fish physiology and biochemistry 13 (1994), S. 353-370 
    Details
    ISSN: 1573-5168
    Keywords: TEM ; SEM ; LM ; chloride cell ; cortisol ; growth hormone ; morphometry ; ion poor water
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The aim of this work was to determine the kinetics of the dramatic development of the gill chloride cells (CCs) during adaptation of the salmonid Oncorhynchus mykiss to an ion-poor environment. To monitor cell division, the incorporation in the mitotic cell DNA of bromo-deoxyuridine (BrdUrd) was visualized with a monoclonal antibody. The density of labelled nuclei was used as an index of cellular division (proliferation), concomitantly with morphometry of phenotypic changes monitored with SEM. In the filament epithelium, a phase of CC differentiation occurred within 12h after the transfer, followed by a delayed phase of cell proliferation (48h). In the lamellar epithelium, the present study demonstrates the absence of cell proliferation after ion-poor water transfer. The conclusion is that proliferation (mitosis) is important in the primary filament whereas differentiation and migration (from the filament) is the main mechanism for the appearance of CCs on the secondary lamellae. The present study suggests that cortisol promoted differentiation, but not division, of cells. CCs, presumably premature, were stained by anti-cortisol monoclonal antibody indicating the presence of cortisol. No mature CCs were stained. Growth hormone (oGH, ratGH) increased the rate of cell division both in lamellar and filament epithelium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00003415
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Determining permeability of coatings of polymer-coated urea (1994)
    Springer
    Nutrient cycling in agroecosystems 38 (1994), S. 47-51 
    Details
    ISSN: 1573-0867
    Keywords: diffusion ; permeability ; polymer-coated urea ; SEM ; urea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Permeability of polymer coatings on urea varies greatly with the type of polymer. A conventional test of measuring the effectiveness of coating involves a 7d static dissolution rate of coated urea into concentrated urea solution, but the results are only qualitative. Our approach was, instead, to make quantitative measurements of permeability, and so make more accurate predictions of release rate of urea across a membrane. A simple device, consisting of a container attached to vertical pipes at the bottom, was constructed to determine permeability of coats on urea granules. A turbulent flow of water ran over a 2 cm pack of coated-urea granules so urea did not accumulate at the outer surface of the coated granules. Separate determinations with two thicknesses of coats (8.8 and 14.7µm) were conducted with water at 12 or 31°C. Permeability and activation energy of permeability were calculated. A comparison was also made between release rate of urea calculated from permeability and that determined by 7d dissolution rate method at 23°C. Nearly 100 h were required for 100% release with the thick coating, but only 20% urea was released after 168 h with the 7d dissolution rate method.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00750061
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    Morphological aspects of subitaneous and resting eggs from Acartia josephinae (Calanoida) (1994)
    Springer
    Hydrobiologia 292-293 (1994), S. 131-135 
    Details
    ISSN: 1573-5117
    Keywords: life cycle ; egg morphology ; Mediterranean Sea ; SEM ; Acartia josephinae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A. josephinae collected along the Salento Peninsula (Ionian and Adriatic Seas) laid two types of eggs, under laboratory conditions, and they are described for the first time. Under a light microscope subitaneous eggs looked smooth on the surface, but scanning electron microscopy revealed that the surface was not perfectly smooth but tubercular with many small protuberances. Resting eggs had long and thick spines whose apical part was multi-branched. The use of egg morphology as a taxonomic character, in the systematic of copepods, is proposed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00229932
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Poly (2,6-dimethyl-1,4-phenylene oxide)/poly (butadiene) diol interpenetrating polymer networks: Phase morphological characteristics and glass transition temperature behavior (1994)
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part A: Polymer Chemistry 32 (1994), S. 257-263 
    Details
    ISSN: 0887-624X
    Keywords: IPNs ; PPO ; poly (butadiene) diol ; SEM ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The fracture surface topography of simultaneous interpenetrating polymer networks (SIN'S) of poly (2,6-dimethyl-1,4 phenylene oxide) (PPO)/poly (butadiene) diol was examined by scanning electron microscopy (SEM). The phase morphology of the IPNs is highly dependent on PPO/poly (butadiene) diol mass ratio. A single-phase morphology of the IPNs was achieved when the content of poly (butadiene) diol is below 40 wt % in the initial composition. The SEM observations are also wholly consistent with the glass transition temperature behavior obtained in the differential scanning calorimetric (DSC) measurements. © 1994 John Wiley & Sons, Inc.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pola.1994.080320206
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 11
    Electronic Resource
    Electronic Resource
    A multi-technique study of the spontaneous oxidation of N-hexane plasma polymers (1994)
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part A: Polymer Chemistry 32 (1994), S. 1399-1414 
    Details
    ISSN: 0887-624X
    Keywords: plasma polymers ; oxidation ; XPS ; FTIR ; SEM ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Long-term compositional changes were observed when n-hexane plasma polymers were stored in air at ambient temperature. These post-deposition changes were monitored over periods exceeding one year using XPS, grazing angle FTIR, and SEM. A rapid initial increase in the oxygen content was followed by a slower oxygen uptake, which continued for many months. In contrast to the reported autocatalytic acceleration of the oxidation of polyolefins, the rate of oxidation of n-hexane plasma polymers was found to decrease continuously, and it did not proceed to the same extent toward higher oxidation states. The plasma polymer coatings did not show physical manifestations of oxidative degradation such as cracking, reduction in thickness, or loss of physical integrity. Comparison of the XPS and FTIR data suggested that the top few nanometers of the plasma polymer coatings oxidized at a faster rate than the “bulk.” The experimental data were interpreted using a model comprising reactions known from the oxidative degradation of polyolefins: carbon-centered radicals, trapped in the course of the plasma deposition, combine with atmospheric oxygen to form metastable peroxy radicals and hydroperoxides, which decompose to generate a variety of stable product groups such as hydroxyl, carbonyl, and carboxylic groups. There was broad agreement between the XPS data and the time dependence of particular species in model calculations. © 1994 John Wiley & Sons, Inc.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pola.1994.080320801
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 12
    Electronic Resource
    Electronic Resource
    The internal compartmentation of rat-liver mitochondria: Tomographic study using the high-voltage transmission electron microscope (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Microscopy Research and Technique 27 (1994), S. 278-283 
    Details
    ISSN: 1059-910X
    Keywords: Matrix ; Membrane ; SEM ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: The three-dimensional organization of the internal compartments of conventionally fixed and embedded rat-liver mitochondria has been determined by tomographic reconstruction from tilt-series images collected on the Albany high-voltage electron microscope. The results indicate that the inner membranes of these organelles are predominantly tubular in the orthodox (expanded matrix) conformation, as previously suggested by scanning electron microscopy. In the condensed (contracted matrix) conformation, the intracristal space opens up into large irregularly shaped compartments which are connected to each other and to the external (intermembrane) space by tubes with approximately the same diameter (20 nm) as those observed in the orthodox state. These results raise several questions, in particular about the nature of the structural transitions that occur in the cristae during matrix expansion and contraction, and about the influence of inner-membrane shape on the diffusion of ions and metabolites between the intracristal and intermembrane compartments. © 1994 Wiley-Liss, Inc.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1070270403
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 13
    Electronic Resource
    Electronic Resource
    Scanning and transmission electron microscopy of clonal peripheral blood lymphocytes in low grade non-Hodgkin's B-cell lymphomas with predominant splenomegaly (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Microscopy Research and Technique 28 (1994), S. 345-355 
    Details
    ISSN: 1059-910X
    Keywords: Lymphoma ; Splenomegaly ; SEM ; TEM ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Peripheral blood mononuclear cells (PBLs) from 14 patients with low grade non-Hodgkin's B-cell lymphomas with predominant splenomegaly were studied by means of scanning (SEM) and transmission electron microscopy (TEM). All patients had peripheral blood and bone marrow involvement, the absence of lymphoadenopathy, and, except in one case, immunophenotypic features of a malignant proliferation of mature spleen B-cells arising from outside the germinal center, but not consistent with CLL or HCL. Several distinctive cytological features were observed in PBLs of the different subgroups. The SEM surface features of PBLs in patients with intermediate differentiation lymphocytic lymphoma (IDL) (five cases), lymphoplasmacytoid immunocytoma (LP-IC) (two cases), and mixed small and large cells malignant lymphoma (one case) were characterized by the presence of numerous well-developed microvilli. Some distinctive TEM ultrastructural features were also seen in the different cases. In the two cases of splenic lymphoma with villous lymphocytes (SLVL), SEM revealed large and elongated surface microvilli generally arising from two or three poles of the cells. This surface morphology, confirmed by TEM analysis, may be pathognomonic of this disease. Four additional cases, tentatively classified as small lymphocytic lymphoma on the basis of immunophenotypic data, were extremely heterogeneous at both SEM and TEM analysis. The ultrastructural features revealed by SEM and TEM may be useful for the more precise characterization of this heterogenous group of diseases, which is generally difficult to define even when immunophenotypic and molecular approaches are used. © 1994 Wiley-Liss, Inc.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1070280409
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 14
    Electronic Resource
    Electronic Resource
    Surface alterations of the bovine oocyte and its investments during and after maturation and fertilization in vitro (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Molecular Reproduction and Development 38 (1994), S. 421-430 
    Details
    ISSN: 1040-452X
    Keywords: Cumulus ; Oocyte ; Zona pellucida ; SEM ; Ultrastructure ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Surface characteristics of the bovine oocyte and its investments before, during, and after maturation, and fertilization in vitro were evaluated by scanning electron microscopy (SEM). Oocyte diameters were also measured during SEM analysis of the oocyte. The cumulus cells manifested a compact structure with minimal intercellular spaces among them in the immature oocytes. These became fully expanded with increased intercellular spaces after maturation in vitro, but contracted again after fertilization. The zona pellucida (ZP) showed a fibrous, open mesh-like structure in the maturing and matured oocytes. The size and number of meshes on the ZP decreased dramatically after fertilization. The vitelline surface of immature oocytes was characterized by distribution of tongue-shaped protrusions (TSPs) varying in density. After 10 and 22 hr of maturation incubation, oocyte surface microvilli (MV) increased to become the predominant surface structure, and TSPs decreased substantially. The vitelline surface of fertilized oocytes (at 6 and 20 hr) was similar to that of the matured oocytes, but unfertilized oocytes had less dense MV than did fertilized oocytes (at 20 hr). The diameter of the oocytes decreased from 99 to 80 μm during maturation and increased to 106 μm after insemination (P 〈 0.05). Membrane maturation was characterized by surface changes from a TSP-predominant pattern to a MV-predominant pattern. Thus, the bovine oocyte maturation process was found to involve the expansion of cumulus cells and the maturation of the ZP, which changes dramatically upon fertilization. Also, volumetric changes occurred in ooplasm processed for SEM following oocyte maturation and insemination. © 1994 Wiley-Liss, Inc.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrd.1080380410
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 15
    Electronic Resource
    Electronic Resource
    Planing frozen hydrated plant specimens for SEM observation and EDX microanalysis (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Microscopy Research and Technique 28 (1994), S. 67-74 
    Details
    ISSN: 1059-910X
    Keywords: Al coating ; Frozen hydrated specimens ; Gels ; Longitudinal sections ; Microanalysis ; Morphometric analysis ; Plant tissues ; SEM ; Serial sections ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: A procedure is described for forming a flat face on a frozen piece of plant tissue, which may then be observed fully-hydrated or lightly etched, and coated or uncoated with a metal film, in scanning electron microscopy (SEM). The frozen sample was planed with a glass knife at -80°C in cryo-ultramicrotome. The sections were discarded, and the planed block face placed on the cold stage in the microscope column, either for observation uncoated at low kV, or for light etching (-90°C) to reveal the cell outlines. If a higher accelerating voltage was needed, the face was given an evaporative coating of Al in the cryo-preparation chamber and returned to the column. The advantages of the planed face over the usual fracture face are illustrated: imaging at a chosen rather than a chance position; clearer cellular and subcellular detail; preservation of hydrated gels like mucilage and swollen cell walls; the possibility of making serial parallel sections through the same piece of tissue; opportunities for accurate morphometric analyses on the planed face; capacity to produce longitudinal sections; preservation of very delicate structures that are destroyed by fixation and drying. A major advantage of the Al-coated planed face is the increased accuracy of energy-dispersive X-ray (EDX) microanalyses on a smooth rather than a rough surface. Tests are included which show that neither the light etching employed, nor successive planing, interferes with the analyses of elements in the frozen face. © 1994 Wiley-Liss, Inc.
    Additional Material: 14 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1070280108
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 16
    Electronic Resource
    Electronic Resource
    Surface expression and distribution of Fc receptor III (CD 16 molecule) on human natural killer cells and polymorphonuclear neutrophils (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Microscopy Research and Technique 28 (1994), S. 277-285 
    Details
    ISSN: 1059-910X
    Keywords: NK cells ; Neutrophils ; Fcγ receptor ; Immunogold ; SEM ; Backscattered electron imaging ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Human natural killer cells and polymorphonuclear neutrophils constitutively express the low-affinity IgG Fc receptor (FcγRIII, CD16 molecule). To investigate cell surface morphology, antigenic receptor density, and topographical distribution of FcγRIII on the plasma membrane of natural killer cells and polymorphonuclear neutrophils, conventional scanning electron microscopy (SEM), flow cytometry, and immunoscanning electron microscopy were used. FcγRIII was detected with an indirect immunogold labeling procedure, and receptors were visualized in the backscattered and secondary electron imaging mode of SEM. Natural killer cells showed a cell surface morphology compatible with lymphocytic differentiation characterized by microvilli and microridges. Polymorphonuclear neutrophils showed surface features characterized by ridges with folds and scattered short microvilli. Natural killer cells displayed a lower cell labeling density, whereas polymorphonuclear neutrophils showed a high level of expression of FcγRIII on the plasma membrane by quantitative analysis with SEM in the backscattered electron imaging mode. Flow cytometry analysis confirmed these findings. Analysis of the topographical distribution of FcγRIII antigenic receptor sites by SEM in the backscattered and secondary electron imaging modes showed that FcγRIII on natural killer cells are randomly distributed, whereas FcγRIII are located on ridges and folds of the plasma membrane of polymorphonuclear neutrophils. These observations suggest that natural killer cells and polymorphonuclear neutrophils differ in their levels of expression and topographic distribution of FcγRIII on the plasma membrane. This different spatial distribution of FcγRIII would provide morphological evidence of certain cellular functions mediated by natural killer cells and polymorphonuclear neutrophils. © 1994 Wiley-Liss, Inc.
    Additional Material: 16 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1070280404
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 17
    Electronic Resource
    Electronic Resource
    Morphological characteristics of boar efferent ductules and epididymal duct (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Microscopy Research and Technique 29 (1994), S. 411-431 
    Details
    ISSN: 1059-910X
    Keywords: Corrosion casts ; LM ; SEM ; TEM ; Microvasculature ; Ultrastructure ; Absorption ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: The aim of the present study was to provide a comprehensive morphological analysis of the porcine epididymis in view of the specific functions being performed in different regions of this organ. Blood supply and microvasculature of efferent ductules and epididymal duct were investigated by means of corrosion casts which were analysed macroscopically and by scanning electron microscopy. This revealed blood supply to the testis and epididymis to be closely related. The capillary pattern was typical for the efferent ductules, the caput, corpus, and distal cauda epididymidis, respectively. Corrosion casts were also used to visualize the course of the efferent ductules themselves. Tissue samples from different regions of the efferent ductules and epididymal duct were examined by light microscopy and both scanning and transmission electron microscopy, with special attention being payed to transitional areas. Morphological criteria allowed the distinction of three segments within the efferent ductules and of the initial segment, proximal caput, distal caput, corpus, proximal cauda, and distal cauda regions of the epididymal duct. Components of the endocytic apparatus of efferent ductule principal cells were identified by ferritin uptake. Ultrastructural evidence of absorption in the epididymal duct was particularly prominent in proximal and distal caput. Extensive cisternae of rough endoplasmic reticulum and a well-developed Golgi apparatus were indicative of active protein synthesis and secretion especially in the distal caput and corpus regions. However, assignment of various organelles in principal cells of the epididymal duct to either absorptive or secretory pathways still remains tentative. © 1994 Wiley-Liss, Inc.
    Additional Material: 53 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1070290603
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 18
    Electronic Resource
    Electronic Resource
    Backscatter electron imaging of double immunogold labeled erythrocytes using two primary monoclonal IgM antibodies (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    Microscopy Research and Technique 28 (1994), S. 286-296 
    Details
    ISSN: 1059-910X
    Keywords: RBC ; SEM ; Colloidal gold ; Silver enhancement ; Double labeling ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: The majority of mouse monoclonal antibodies reacting with blood group epitopes on erythrocytes are of the IgM class, have equal light chain type, and are available as culture supernatants only. To study the interrelationship of the blood group antigens, a method is presented which allows double labeling applying two unconjugated monoclonal antibodies of the same class and species. The method comprises two indirect, sequential labelings using mouse IgM anti-A and anti-H as primary antibodies and two goat anti-mouse IgM conjugated to 30 and 20 nm colloidal gold particles as secondary antibodies. After labeling for the first antigen, free binding sites on the primary antibody are blocked by incubation with an unconjugated goat anti-mouse antibody. The free anti-species on the secondary antibody, conjugated to 30 nm gold particles, are inactivated by silver enhancement. The silver enhancement also enlarges the gold particle for optimal discrimination between the two particle sizes, which are chosen accordingly. Semiquantitations of double labeled cells from subgroup A2 and A3 were found to be in good agreement with the counts of the corresponding single labelings as well as between experiments, irrespective of which of the two antibodies was applied in the first labeling sequence. The results were in accordance with a reciprocal but nonlinear relationship between the A and H antigens and suggest different affinities of the two antibodies for the epitopes in the subgroups investigated, indicating different biochemistry of the antigen determinants. © 1994 Wiley-Liss, Inc.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1070280405
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 19
    Electronic Resource
    Electronic Resource
    Preparation and characterization of CaTiO3-based perovskitic oxides as catalysts for partial oxidation of light hydrocarbons (1993)
    Springer
    Catalysis letters 18 (1993), S. 37-48 
    Details
    ISSN: 1572-879X
    Keywords: Perovskites ; oxidative coupling of methane ; XRD ; SEM ; TGA ; BET ; CaTiO3-based catalyst ; sol-gel method
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A series of perovskites of the formula Ca1−x Sr x Ti1−y M y O3−δ, M=Fe, Co, Cr or Ni,x = 0−1,y = 0−0.6, has been synthesized by a modified sol-gel method using citrate. Several of these materials were proved to be stable under operating conditions in reducing atmospheres of air and hydrocarbons. An outline of the synthesis procedure is given, together with the results of XRD, SEM, BET, TG, DTA and IR characterization before and after catalytic testing. The solubility of Ni and Cr in this perovskite was very limited, and the solubility of Co decreased abruptly above 1173 K. The solubility range of Ca and Sr on alkaline earth sites is 100%.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00769496
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 20
    Electronic Resource
    Electronic Resource
    SEM observations on Seison and phylogenetic relationships of the Seisonidea (Rotifera) (1993)
    Springer
    Hydrobiologia 255-256 (1993), S. 513-520 
    Details
    ISSN: 1573-5117
    Keywords: evolution ; mastax ; morphology ; phylogeny ; Rotifera ; Seison ; Seisonidea ; SEM ; trophi
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The trophi and external features of the marine rotifer, Seison, are described using SEM. Organization of the trophi of the Seisonidea is discussed and possible evolutionary origins of the sclerite system of the fulcrate mastax are presented. Additional information on the taxonomy and general ecology of Seison are offered.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00025880
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 21
    Electronic Resource
    Electronic Resource
    Copper/Solder intermetallic growth studies (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Microscopy Research and Technique 25 (1993), S. 503-508 
    Details
    ISSN: 1059-910X
    Keywords: Intermetallic compounds ; ESEM ; SEM ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Copper samples, hot solder (eutectic) dipped and thermally aged, were cross-sectioned and placed in an environmental scanning electronic microscope (ESEM). While in the ESEM the samples were heated for ∼ 2.5 h at 170°C to stimulate the growth of additional Cu/Sn intermetallic compound. The intent of the study was to obtain a continuous real-time videotape record of the diffusion process and compare the observations to static SEM images reported to represent long-term, naturally aged intermetallic growth. The video obtained allows the observation of the diffusion process and relativistic growth phenomena at the Cu, Cu3Sn, Cu6Sn5, and solder interfaces as well as effects on the bulk Cu and solder. Effects contrary to earlier reports were observed; for example, growth rates of Cu3Sn were found to greatly exceed those of Cu6Sn5. © 1993 Wiley-Liss, Inc.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1070250522
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 22
    Electronic Resource
    Electronic Resource
    Quantitative analysis and cartography in scanning electron microscopy: Application to the study of bacterial adhesion to respiratory epithelium (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Microscopy Research and Technique 24 (1993), S. 527-536 
    Details
    ISSN: 1059-910X
    Keywords: Image analysis ; Respiratory cells ; SEM ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: This paper describes the coupling between a scanning electron microscope (SEM) and an image analysis workstation. The system was designed in order to drive the SEM and to analyse any sample. It allows automatic (edge detection) or semiautomatic (pointing, marking, drawing) object detection. Two types of data can be obtained: (1) topographical information, such as the location of the object within a region of interest drawn at any magnification of the microscope, or (2) quantitative data, such as morphometric characteristics of objects. In addition, high resolution maps of the section, regions of interest, and objects can be obtained with a laser printer. This software was first applied to quantitate the adhesion of the bacteria Pseudomonas aeruginosa to human respiratory epithelial cells in culture. P. aeruginosa was shown associated with ciliated cells. The second application concerned the study of the distribution of specific carbohydrate residues at the surface of the respiratory cells. The gal residues were revealed using the lectin Ricinas communis agglutinin II, adsorbed to colloidal gold particles. A relationship between the presence of adherent bacteria and labelling was shown. © 1993 Wiley-Liss, Inc.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1070240610
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 23
    Electronic Resource
    Electronic Resource
    Ultrastructural evidence for a binding substance to the sweet-tasting protein thaumatin inside taste bud pores of rhesus monkey foliate papillae (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Microscopy Research and Technique 26 (1993), S. 133-141 
    Details
    ISSN: 1059-910X
    Keywords: Taste ; Secretion ; Microvilli ; Freeze-substitution ; Freeze-fracture deep-etching ; Electron microscopic cytochemistry ; Colloidal gold ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Thaumatin is a protein that tastes intensely sweet only to Old World monkeys and to higher primates, including man. Here we used pre-embedding ultrastructural methods to study the distribution of thaumatin in apical regions of Rhesus monkey foliate papillae, using thaumatin conjugated to 5 nm gold particles. With freeze-substitution we saw that gold-labeled thaumatin bound to an electron-opaque, sponge-like secretory substance inside the taste bud pores. Labeled thaumatin was found at the surface of the secretory substance even deep inside the pore, where other, unlabeled cellular structures surrounded the substance. With freeze-fracture deep-etching the secretory substance that bound the thaumatin-gold particles appeared coarsely granular. There was no labeling of any other taste bud pore structure, including microvilli and small membranelined vesicles. Pre-incubation with an excess of unlabeled thaumatin inhibited binding with goldlabeled thaumatin. The results suggest that the secretory substance had the greatest affinity of all taste pore structures to the sweet-tasting compound under our experimental conditions. Therefore, gustatory reception probably involves various taste compound binding structures, microvilli, and also secretory substances like the one described here which bound thaumatin. We speculate that the secretory substance may bind taste stimuli and serve as an intermediate between stimuli and receptors. It could be involved in stimulus removal or delivery or both. © 1993 Wiley-Liss, Inc.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1070260206
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 24
    Electronic Resource
    Electronic Resource
    MAS NMR studies of ZSM-5 zeolites: Correlation to para selectivity and SEM observations (1992)
    Springer
    Catalysis letters 14 (1992), S. 349-357 
    Details
    ISSN: 1572-879X
    Keywords: Solid state29Si and27Al NMR ; zeolites ; SEM ; selectivity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract 27Al and29Si MAS NMR studies of various ZSM-5 zeolites of different Si/Al ratio, crystal size synthesized with and without alkali metal salt addition by the hydrothermal technique were carried out. The NMR observations clearly showed that the technique can be employed to differentiate Si/Al ratio, framework tetrahedral content and the crystal size. The last observation has been confirmed by SEM and catalytic activity test for para ethylation of ethylbenzene. A good correlation was found between the catalytic activity and the data from NMR and SEM.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00769673
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 25
    Electronic Resource
    Electronic Resource
    Methane oxidative coupling over Na2WO4/SiO2 (1992)
    Springer
    Catalysis letters 13 (1992), S. 221-228 
    Details
    ISSN: 1572-879X
    Keywords: Oxidative coupling of methane ; metal oxides ; XRD ; XPS ; SEM ; LRS ; Na2WO4/SiO2 catalyst
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Methane oxidative coupling (MOC) was studied over Na2WO4/SiO2. The effect of Na2WO4 loading and reaction conditions on the catalytic behaviour was investigated. XRD, SEM, LRS and XPS have been used to study the catalyst morphology, Na2WO4 dispersity and surface oxygen species. These results were correlated with the catalytic activity and selectivity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00770994
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 26
    Electronic Resource
    Electronic Resource
    Information on catalyst surface structure from crystallite morphologies observed by scanning electron microscopy (SEM) (1992)
    Springer
    Catalysis letters 15 (1992), S. 123-131 
    Details
    ISSN: 1572-879X
    Keywords: Computer simulation ; SEM ; catalyst modelling ; Cr2O3 ; crystal morphology ; surface segregation ; chromia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The shapes of small crystals grown under equilibrium conditions are governed by the stabilities of their exposed faces. Computer simulation methods can be used to calculate surface energies and hence crystallite morphologies, illustrated by results for Cr2O3. Such calculations can include the effects of surface impurity segregation. Comparison of the resulting calculated crystal morphologies with those directly observed in the SEM thus provides a direct link between atomistic simulation and experiment.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00770905
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 27
    Electronic Resource
    Electronic Resource
    Expression of ogu cytoplasmic male sterility in cybrids of Brassica napus (1992)
    Springer
    Theoretical and applied genetics 83 (1992), S. 549-556 
    Details
    ISSN: 1432-2242
    Keywords: Cytology LM ; SEM ; TEM ; Cybrid ; Rapeseed ; Radish ; Cytoplasmic male sterility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A light and electron microscopic investigation revealed that ogu cytoplasmic male sterility (CMS) in cybrids of Brassica napus is primarily a deficiency of the tapetum and clearly time and site specific. Three patterns of ogu CMS were found, and specific conclusions drawn. First, the partially male fertile cybrid 23 was highly variable. It sometimes produced heterogeneous stamens with an endothecium formed exclusively around the fertile locules, thus delineating each microsporangium as a functional unit. The second type, including cybrids 27, 58 and 85, on the contrary, was stable and completely male sterile. In the four locules of normal length, microspores were observed to die at the vacuolate polarized stage while the tapetum disappeared prematurely through excessive vacuolization by the end of meiosis followed by a rapid autolysis during the tetrad or early free microspore stage. The subepidermal layer of the locule wall failed to form characteristic thickenings. The male-sterile stamens were completely indehiscent. At the time of anthesis they contained only collapsed empty exines adhering to each other. These cybrids, 27, 58 and 85, were closest to the ogu CMS trait of radish and seemed to be the best suited for further use in plant breeding. The third pattern was found in cybrids 77 and 118, which besides showing abortion of the microsporangia also showed a feminization of the stamens. We suggest that this feminization might be due to an alloplasmic situation associating Brassica napus nuclear genes with the mitochondrial DNA of radish.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00226898
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 28
    Electronic Resource
    Electronic Resource
    Limits for the detection of daily otolith increments in whitefish (Coregonus lavaretus L.) larvae (1992)
    Springer
    Hydrobiologia 231 (1992), S. 99-105 
    Details
    ISSN: 1573-5117
    Keywords: Coregonus ; otolith ; daily increments ; resolution limit ; SEM
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The formation of daily otolith increments in European lake white-fish was evaluated in the laboratory during 40 days at 4, 6, and 8 °C under limited and ad libitum food supply. Daily increments were easily identified in larvae reared at 8 °C. At 6 °C and, more, at 4 °C, an unstructured perinuclear zone was formed, and daily increments could only be recognized in the light microscope starting from 10 to 35 days after hatching. In some larvae from the 4 °C groups, no increments at all were found. Scanning electron microscopy (SEM) could not improve increment resolution. Only those increments could be viewed by SEM which were visible in the light microscope as well. We conclude that whitefish larvae which experience low (4–6 °C) temperatures during their first weeks of life, hence those which hatch in the lake, cannot be aged by the currently employed preparation techniques.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00006502
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 29
    Electronic Resource
    Electronic Resource
    The ecology and distribution of the littoral freshwater Chydoridae (Branchiopoda, Anomopoda) of Ireland, with taxonomic comments on some species (1992)
    Springer
    Hydrobiologia 241 (1992), S. 1-70 
    Details
    ISSN: 1573-5117
    Keywords: Chydoridae ; chydorid ecology ; Ireland ; biogeography ; SEM
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This paper is the first comprehensive review of the littoral freshwater Chydoridae (Branchiopoda, Anomopoda) of Ireland. It reports on a countrywide survey, during which a total of 316 samples was taken at 287 sampling sites between March, 1984 and June, 1986. Together with all previous records, the survey results provide baseline data on the ecology and distribution of this important animal group. A total of 41 taxa in 18 genera of the Family Chydoridae has been recorded in Ireland. Several taxa exhibit morphological differences from original descriptions and populations from other geographical regions, and the taxonomic significance of this variation is assessed. All the taxa encountered during this survey are illustrated by scanning electron micrographs. The distribution of each taxon is given and observations are made on regional trends.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00007749
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 30
    Electronic Resource
    Electronic Resource
    The unique headpore of Monospilus dispar (Cladocera, Chydoridae) is not lonesome: new record of minute lateral pores (1992)
    Springer
    Hydrobiologia 232 (1992), S. 145-148 
    Details
    ISSN: 1573-5117
    Keywords: headshield ; phylogeny ; SEM ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The headshield of Monospilus dispar (Cladocera, Chydoridae, Aloninae) was characterized by the presence of a unique headpore. Scanning Electron Microscopy shows the occurrence of minute pores close to the main headpore. The intimate structure of the main headpore is formed by concentric lamellae. This new information leads to new considerations about phylogeny.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00017473
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 31
    Electronic Resource
    Electronic Resource
    The flocculation of wine yeasts: biochemical and morphological characteristics inZygosaccharomyces — flocculation inZygosaccharomyces (1992)
    Springer
    Antonie van Leeuwenhoek 61 (1992), S. 317-322 
    Details
    ISSN: 1572-9699
    Keywords: flocculation ; proteinases ; SEM ; Zygosaccharomyces bailii ; Zygosaccharomyces fermentati
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The floc-forming ability of flocculent strains ofZygosaccharomyces bailii andZ fermentati, isolated from musts, was tested for susceptibility to proteinase and sugar treatments.Z. fermentati was found highly resistant to the proteolytic enzymes tested, whereasZ. baili was only trypsin-resistant. The inhibition of flocculation by sugars distinguished two types: inZ. fermentati flocculation was completely inhibited by mannose, inZ. bailli by various sugars. By SEM observation, the cell surface ofZygosaccharomyces revealed the presence of a column structure, resulting from fusion of vesicles present on the cell surface.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00713939
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 32
    Electronic Resource
    Electronic Resource
    The characterisation of GaAs on silicon using polarised laser light (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    Advanced Materials for Optics and Electronics 1 (1992), S. 189-195 
    Details
    ISSN: 1057-9257
    Keywords: Ellipsometry ; GaAs ; Silicon ; Surface roughness ; MBE ; SEM ; Rotating polariser multiple-angle-of-incidence ellipsometer ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Electrical Engineering, Measurement and Control Technology , Physics
    Notes: MBE-grown gallium arsenide epitaxial layers on silicon, with thicknesses between 0.1 and 8.1 μm, have been studied using a simple rotating polariser multiple-angle-of-incidence ellipsometer. From these data, information on the roughness of the surfaces of the layers and the anisotropy of the refractive index of the layers has been obtained. The results are compared with data obtained on the same samples using scanning electron microscopy and conventional spectroscopic ellipsometry.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/amo.860010405
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 33
    Electronic Resource
    Electronic Resource
    Ultrastructural imaging of freeze-fractured plant cells in the scanning electron microscope (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    Microscopy Research and Technique 22 (1992), S. 160-169 
    Details
    ISSN: 1059-910X
    Keywords: Freeze-fracture and cytoplasmic maceration ; Chloroplasts ; Pollen ontogeny ; SEM ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: The application of the freeze-fracture and cytoplasmic maceration technique in ultrastructural studies of plant cells is described. A major advantage of the technique is, that by extracting mobile cytoplasmic components from the freeze-fractured cells, surface relief is introduced and three-dimensional information is obtainable. The details of specimen preparation are described and the results obtained are reviewed. The use of chitosan embedding for very small or fragile specimens is described. © 1992 Wiley-Liss, Inc.
    Additional Material: 21 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1070220205
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 34
    Electronic Resource
    Electronic Resource
    Atypical cells in the normal guinea pig organ of Corti as revealed by micromanipuiation in SEM (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    Microscopy Research and Technique 20 (1992), S. 288-297 
    Details
    ISSN: 1059-910X
    Keywords: SEM ; TEM ; Guinea pig ; Cochlea ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Inner ear tissue of the normal guinea pig was conductively stained (OTOTO-method) for SEM investigations. The Hensen's cells of the organ of Corti were removed using a micromanipulator inside the SEM. By this method atypical bodies of sensory and supporting cells were revealed in the apical turns of the cochlea. Atypical sensory cells showed great variations in size and shape. Several had no contact to Deiter's cells and no or only one nerve supply at their basal end. Atypical Deiter's cells showed alterations in shape and in the form of their phalangeal processes.Additionally altered parts of the organ of Corti were isolated by micromanipulation and embedded for correlative TEM-investigations.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1070200309
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 35
    Electronic Resource
    Electronic Resource
    Changes in the interior surface of newly mesodermalized ectoderm and its contact activity with competent ectoderm in the newtCynops (Amphibia) (1991)
    Springer
    Development genes and evolution 199 (1991), S. 413-422 
    Details
    ISSN: 1432-041X
    Keywords: Mesodermalization ; Neural induction ; Amphibia ; Cell contact ; SEM
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The presumptive ectoderm (pE) ofCynops gastrulae was artificially mesodermalized by contact with teleost swimbladder. The newly mesodermalized ectoderm (mE) acquired the capacity for neural induction (Suzuki et al. 1986a). SEM observations revealed that the mE cells altered their cellular profiles immediately after mesodermalization. The characteristics of the cell surface and the cell architecture became similar to those of invaginated mesoderm cells. There were distinct differences in the cellular contact between mE—pE and pE—pE combinations. The mE-pE combinations kept close contact at their interior surfaces, while the pE—pE combinations did not keep contact. Both TEM and SEM observations also indicated that there were tight contacts between mE and pE cells. These findings suggest that neural-inducing activity of the newly mesodermalized ectoderm cells is coupled with acquisition of cellular affinity toward the interior surface of competent ectoderm cells, and probably requires close cell contacts.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01705852
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 36
    Electronic Resource
    Electronic Resource
    Immunocytochemical and ultrastructural characterization of endocrine cells in chicken proventriculus (1991)
    Springer
    Cell & tissue research 263 (1991), S. 541-548 
    Details
    ISSN: 1432-0878
    Keywords: Proventriculus ; Endocrine secretory cells ; Secretory granules ; Peptide hormones ; Colocalization ; Immunocytochemistry ; Colloidal gold ; Chicken
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The endocrine cells of the chicken proventriculus were investigated immunocytochemically, using the peroxidase-antiperoxidase technique on paraffin and semithin sections for light microscopy, and immunogold staining in osmium-fixed material for electron microscopy. The fixation procedure also allowed a detailed ultrastructural investigation. Twenty-three antisera were tested and 7 immunoreactive cell-types were identified: D-cells containing somatostatin-like peptide; EG-cells immunoreactive to anti-glucagon, anti-GLP1 and antineurotensin; NT-cells labelled only with anti-neurotensin; BN-cells containing bombesin-like material; ENK-cells showing met-enkephalin immunoreactivity; EC-cells reactive to anti-serotonin; and APP-cells positive to anti-avian pancreatic polypeptide. In addition, enterochromaffin-like (ECL) cells, were also detected by electron microscopy. The presence of ENK-cells and the ultrastructure of these and NT-cells are described for the first time in chicken proventriculus, and glucagon, GLP1 and neurotensin are shown to be colocalized in the EG-cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00327287
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 37
    Electronic Resource
    Electronic Resource
    Membrane routing during exocytosis and endocytosis in neuroendocrine neurones and endocrine cells: use of colloidal gold particles and immunocytochemical discrimination of membrane compartments (1991)
    Springer
    Cell & tissue research 264 (1991), S. 299-316 
    Details
    ISSN: 1432-0878
    Keywords: Neurosecretosomes ; Pituitary ; Microvesicles ; Vacuoles ; Endocytosis ; Colloidal gold ; Rat (Long Evans, Brattleboro)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The hypothesis that the retrieval of membranes of neurohypophysial neurosecretory granules (NSG) and small electron-lucent microvesicles occurs by different routes was tested by incubating neurohypophysial neurosecretosomes with colloidal gold particles of various sizes. Neurosecretosomes derived from normal Long Evans rats and incubated in media of normal ionic composition endocytosed a few small (〈25 nm) gold particles into 40–50 nm electron-lucent microvesicles. After depolarisation, more small gold particles were found in microvesicles, and small and large (〉25 nm) gold particles in vacuoles. Oxytocin-containing neurosecretosomes derived from Brattleboro rats, which contain 160 nm-diameter NSG, endocytosed gold particles in a pattern indistinguishable from that of neurosecretosomes from Long Evans rats. However, neurosecretosomes derived from defective vasopressin neurones of Brattleboro rats, which contain microvesicles, small vacuoles, and a few 100 nm dense-cored vesicles, but not 160 nm NSG, endocytosed only small colloidal gold particles. Early after depolarisation the gold particles were present only in microvesicles, but later some could be found in vacuoles and lysosome-like structures. Immunogold cytochemistry using a polyclonal antiserum raised against microvesicle-rich neurosecretosomes derived from Brattleboro rats labelled microvesicles in the posterior pituitary strongly, NSG weakly, and vacuoles to a variable extent. These data together indicate that, after exocytosis, the membranes of NSG are recaptured as large vacuoles. Microvesicles are exocytosed and endocytosed separately.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00313967
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 38
    Electronic Resource
    Electronic Resource
    Nematocysts of the Mediterranean hydroid Halocordyle disticha (1991)
    Springer
    Hydrobiologia 216-217 (1991), S. 607-613 
    Details
    ISSN: 1573-5117
    Keywords: hydroid ; SEM ; nematocysts ; stenoteles ; desmonemes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The morphology, distribution and function of nematocysts in the hydroid Halocordyle disticha were studied by light and scanning electron microscopy. Two types of stenoteles, two types of microbasic b-mastigophores (one with a capsular inclusion) and one type of desmoneme were identified. Prey-capturing experiments were performed with Artemia sp. nauplii. Stenoteles and microbasic mastigophores (those with inclusions) penetrated and paralyzed Artemia larvae, whereas desmonemes kept them in a firm grip by winding around setae.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00026520
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 39
    Electronic Resource
    Electronic Resource
    Food ingestion by planarian intestinal phagocytic cells — a study by scanning electron microscopy (1991)
    Springer
    Hydrobiologia 227 (1991), S. 179-185 
    Details
    ISSN: 1573-5117
    Keywords: planarian ; intestine ; endocytosis ; phagocytosis ; tissue culture ; SEM
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Tissue-cultured fragments of the intestine of Dugesia japonica were examined by SEM for formation of pseudopodia in the phagocytic cells. For comparison, parallel experiments in vivo were performed using intact animals under starved and fed conditions. When cultures were incubated with fetal calf serum, individual phagocytic cells formed one or two large cup-like pseudopodia. Cultures incubated with latex beads showed pseudopodia that varied in shape according to the size of the beads and that had multiple folds or funnels. Pseudopodia and the modes of their endocytosis in this planarian, however, are morphologically like those of other phagocytes such as digestive cells of Hydra, rat macrophages, and human neutrophils. Culture in vitro of digestive tissue should be an important tool for studying the digestive physiology of planarians at the cellular level.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00027600
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 40
    Electronic Resource
    Electronic Resource
    Microwave energy fixation of plant tissue: An alternative approach that provides excellent preservation of ultrastructure and antigenicity (1991)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 17 (1991), S. 81-94 
    Details
    ISSN: 0741-0581
    Keywords: Colloidal gold ; Pathogenesis-related (PR) proteins ; Tobacco plants ; Hypersensitivity ; Tobacco mosaic virus ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Immunocytochemical techniques are confronted with the problem of obtaining adequate tissue preservation together with retention of protein antigenicity. Various methods, including freeze-drying and freeze-substitution, have been devised to circumvent this problem. In the present study, we report that microwave energy used in combination with low concentrations of glutaraldehyde (0.1%) and paraformaldehyde (2%) preserves the structural integrity of plant tissue and antigenicity of proteins. Tobacco leaf samples fixed in a time as brief as 15-20 s exhibited excellent preservation of fine structures. By contrast, specimens irradiated for shorter (5-10 s) or longer (30-40 s) periods showed poor morphological preservation. Microwave irradiation for 15-20 s was found useful for immobilizing large amounts of soluble antigens. The fast microwave fixation method was successfully used to preserve pathogenesis-related (PR) proteins, which were subsequently localized by a postembedding immunogold procedure. In addition to soluble antigens, cellulose subunits and pectic substances, two major plant cell wall components, were found to be highly preserved in microwave-irradiated tobacco plant tissue. The present study demonstrates that microwave fixation of plant tissue is a simple and inexpensive method that is easy to perform with commercially available microwave ovens. The incubation time for fixation is reduced from 2 h to 15-20 s without loss of fine structural details. This method will undoubtedly acquire increasing applicability and relevance in plant biology.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060170109
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 41
    Electronic Resource
    Electronic Resource
    Immunogold labelling of the intermediate filament-lamina-nuclear matrix system in hela and bhk-21 cells (1991)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 18 (1991), S. 126-134 
    Details
    ISSN: 0741-0581
    Keywords: Sequential extraction ; Whole-mount cell ; Monoclonal antibody ; Colloidal gold ; Cross-reaction ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Whole-mount, sequentially extracted cells combined with immunogold electron microscopy were developed to demonstrate the intermediate filaments, lamina, and nuclear matrix (IF-L-NM) and to identify their protein components. The IFs of HeLa cells were reacted both with keratin and vimentin monoclonal antibodies; meanwhile, the IF network of BHK-21 cell was reacted only with vimentin monoclonal antibody. The lamina and nuclear matrices of both HeLa and BHK-21 cell were labelled, respectively, with lamin monoclonal antibody-gold complex and 280 Kd nuclear matrix protein monoclonal antibody-gold complex. The monoclonal antibody to keratin could cross-react with the lamina both of HeLa and BHK-21 cells.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060180206
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 42
    Electronic Resource
    Electronic Resource
    Ultrastructural localization of gold particles within neural grafts labeled with gold-filled sendai viral envelopes (1991)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 18 (1991), S. 197-202 
    Details
    ISSN: 0741-0581
    Keywords: Graft ; Transplant ; Labeling ; Colloidal gold ; Sendai virus ; Cortex ; Nucleus basalis ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: The ability to discriminate between host and donor cells is required to interpret the organization of neural grafts at the electron microscopic (EM) level. Using light microscopy, Ardizzoni et al. (Ardizzoni, S.C., Michaels A., and Arendash, G.W. [1988] Science 239: 635-637) described a method, using gold-filled Sendai viral envelopes, for labeling cell suspensions prior to grafting. As the colloidal gold used in this procedure is especially attractive for use with EM, we have examined the ultrastructural distribution and character of this label with transplanted cells. Cell suspensions taken from the nucleus basalis of fetal rats were labeled using gold-filled Sendai viral envelopes and grafted into the dorsal neocortex of adult host rats with nucleus basalis lesions. After varying survival times ranging from 1 to 14 months, grafts and surrounding host tissue were examined using standard EM techniques. Within the graft site, gold particles ranging from 10-200 nm were found associated with various membranes throughout the cytoplasm of both neurons and glia. Gold particles of similar size were also found within the nuclei of neuronal and non-neuronal cells. Host cells near the graft site contained some small gold particles (10-40 nm). Control injections of non-viable, gold-labeled cells or colloidal gold alone resulted in similar patterns of small gold particles which were readily discriminable from the larger virally inserted gold particles found in viable labeled donor cells. We conclude that this method allows discrimination between closely associated host and donor cells.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060180216
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 43
    Electronic Resource
    Electronic Resource
    Tridimensional ultrastructure of perfusion fixed gastrointestinal epithelial cells by high resolution scanning electron microscopy (1991)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 18 (1991), S. 223-230 
    Details
    ISSN: 0741-0581
    Keywords: SEM ; Intestinal morphology ; Intracellular structure ; Mitochondria ; Cell membrane ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Improvements in the design of modern scanning electron microscopes (SEM) and new methods of specimen preparation incorporating chemical removal of the cytosol and cytoskeleton, now make it possible to view cells and their organelles in three dimensions (3D) at high magnification. In this experiment, high resolution SEM (HRSEM) utilizing new methods of tissue preparation was used to study the intracellular structures of the mouse ileum. In addition, in vivo intestinal perfusion was used to further enhance cellular preservation. Using these modifications it was possible to visualize, in 3D, the fine structure of intestinal epithelial cells and intracellular organelles such as the nucleus, mitochondria, endoplasmic reticulum, and Golgi complex, as well as microvilli and cell membrane. Whole mitochondria appeared as irregularly shaped organelles which contained tubular cristae. Plate-like cristae were not observed. The brush border was found to be a closely packed array of cylindrical projections. The extensive folding and structural intricacy of lateral cell membranes between absorptive cells could only be appreciated by viewing this tissue with 3D HRSEM. The use of HRSEM to study 3D ultrastructure of cells and their organelles will improve our understanding of the structure-function relationships in both the healthy and diseased gastrointestinal tract.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060180304
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 44
    Electronic Resource
    Electronic Resource
    Immunoalkaline phosphatase with cerium-based cytochemistry for double staining at the transmission electron microscopic level (1991)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 18 (1991), S. 121-125 
    Details
    ISSN: 0741-0581
    Keywords: Immunocytochemistry ; Colloidal gold ; Cerium chloride ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Immunoelectron microscopic localization of surface antigens on lymphocytes is possible using alkaline phosphatase combined with cerium-based cytochemical methods. Distinctive electron-dense deposits are easily identified at sites of antibody binding. Mouse splenocytes showing surface immunoglobulin localization and human peripheral blood lymphocytes stained for the MHC-Class II antigen HLA-DR illustrate the results. Double staining for murine Ia antigen by the alkaline phosphatase procedure, combined with immunogold labeling of antigens identified on dendritic cells, i.e., NLDC-145, demonstrates the utility of the cerium cytochemical method.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060180205
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 45
    Electronic Resource
    Electronic Resource
    An alkali digestion method to expose connective tissue fibers: A scanning electron microscopy study of rat lung (1991)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 19 (1991), S. 486-490 
    Details
    ISSN: 0741-0581
    Keywords: Alveoli ; SEM ; Stereo pair images ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Alkali digestion has been used to remove cellular elements of tissues thus exposing the underlying connective tissue framework. We studied the action of this severe alkali treatment on the delicate tissues of rat lung. The lungs of male Sprague-Dawley rats were perfused with saline to remove blood and then inflated by fixation through the airways at 20 cm pressure. Sections of lung 2 × 5 × 5 mm were immersed in 2.5 M NaOH at 25°C for 6 h, 16 h, 24 h, 48 h, and 72 h. The alkali was changed daily. Tissues were washed to neutral with water (24 h), treated with tannic acid (1%, 3h), post-fixed with osmic acid (1%, 3 h) and processed for SEM. At 6 h, epithelial cells started to peel off the alveolar surface. At 16 h the digestion process was well advanced. At 48 h the cells were completely removed revealing the lattice network of connective tissue fibers within the alveolar surface. The method allows the complete removal of cellular elements of the lung while retaining the very fine 3D structure of the connective tissue matrix.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060190411
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 46
    Electronic Resource
    Electronic Resource
    Characterization of oxide structures formed on nickel-chromium alloy during low pressure oxidation at 500–600°C (1990)
    Springer
    Oxidation of metals 33 (1990), S. 457-479 
    Details
    ISSN: 1573-4889
    Keywords: Ni-Cr alloys ; transient oxide structures ; quantitative XPS ; Auger ; TEM ; SEM
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Low pressure oxidation studies of Ni-18%Cr alloy were carried out at temperatures of 500–600°C for very brief periods. Detailed XPS, AES, SEM, and TEM studies identified four stages in the initial oxidation. These are: (1) formation of a mixed nickel-chromium oxide overlayer; (2) growth of submicron-sized oxide nodules; (3) development of dark “hole-like” patches on the surface; and (4) growth of “second generation” oxide nodules. Both types of nodules consist primarily of a nickel structure depleted in oxygen. Their formation appears to result from a very rapid outward movement of nickel from localized defects in the metal. The dark patches result from the presence of a chromium oxide-rich underlayer, which appears to form by a lateral migration of chromium from adjacent oxide/metal interface regions and from grain boundaries.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00666809
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 47
    Electronic Resource
    Electronic Resource
    The first nauplius and the copepodite stages ofThalestris longimana Claus, 1863 (Copepoda, Harpacticoida, Thalestridae) and their bearing on the reconstruction of phylogenetic relationships (1990)
    Springer
    Hydrobiologia 202 (1990), S. 33-60 
    Details
    ISSN: 1573-5117
    Keywords: Postembryonic development ; morphogenesis ; SEM ; ecology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The first nauplius and 5 copepodite stages ofThalestris longimana Claus, 1863 are described. Sexual dimorphism makes its first appearance in the copepodid IV. Brief remarks are given on cultivation and on ecology of the species. The significance of both the naupliar and the copepodite development for the reconstruction of phylogenetic relationships of Thalestridae is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02208126
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 48
    Electronic Resource
    Electronic Resource
    The first nauplius and the copepodite stages of Thalestris longimana Claus, 1863 (Copepoda, Harpacticoida, Thalestridae) and their bearing on the reconstruction of phylogenetic relationships (1990)
    Springer
    Hydrobiologia 202 (1990), S. 33-60 
    Details
    ISSN: 1573-5117
    Keywords: Postembryonic development ; morphogenesis ; SEM ; ecology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The first nauplius and 5 copepodite stages of Thalestris longimana Claus, 1863 are described. Sexual dimorphism makes its first appearance in the copepodid IV. Brief remarks are given on cultivation and on ecology of the species. The significance of both the naupliar and the copepodite development for the reconstruction of phylogenetic relationships of Thalestridae is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00027091
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 49
    Electronic Resource
    Electronic Resource
    Scanning electron microscopy of nerve fibers in human fetal cochlea (1990)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 15 (1990), S. 104-114 
    Details
    ISSN: 0741-0581
    Keywords: Nerve fiber ; Afferent and efferent nerves ; Cochlea ; Fetal inner ear ; Human ear ; SEM ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: The cochleas of four human fetuses ranging 22-25 weeks gestation were studied by scanning electron microscopy (SEM) for the purpose of obtaining a better understanding of the nerve fiber arrangement in the human ear. After critical point drying, the specimens were dissected and the floor of the tunnel of Corti and the outer wall of Nuel's space were exposed for observation. Upper cochlear turns, especially the apical turn, seemed to be still immature.Observed nerve fibers were classified into three types:1Spiral fibers: Fibers traveling basalward and following the shape of the cochlea were found in both the tunnel of Corti and Nuel's space and believed to be the afferent nerves responsible for innervating the outer hair cells2Radial fibers: radiating outward from the osseous spiral lamina - one such radial fiber transversing high in the tunnel space (supposedly the efferent nerve servicing the outer hair cells), and another sort of radial fiber (found crossing the tunnel floor), the nature of which was uncertain.3Irregular fibers: Consisting of thin, randomly running fibers within the cochlea. The destination of these fibers was not determined, but possibly they represent transitory nerve branchings of afferent or more probably efferent nerves, which would later regress during maturation.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060150203
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 50
    Electronic Resource
    Electronic Resource
    Scanning electron microscopic study of immunogold-labeled human leukocytes (1990)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 14 (1990), S. 298-306 
    Details
    ISSN: 0741-0581
    Keywords: Colloidal gold ; Critical point drying ; Hairy cell leukemia ; Interleukin-2 receptor ; Macrophages ; Lymphokine-activated killer cells ; Peldri II ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: For many years critical point drying (CPD) has been the method of choice for preparing cells for scanning electron microscopy (SEM). Described herein is a simple, efficient, inexpensive, reproducible, and safe procedure using Peldri II, a proprietary fluorocarbon compound that is solid at room temperature and a liquid above 25°C, as a sublimation dehydrant for processing specimens for SEM. The utility of Peldri II was demonstrated in studies using leukocytes from the blood of healthy donors and patients with leukemia as well as from long-term lymphoblastoid cell lines. The application of the proposed Peldri II procedure was further documented in SEM studies in which the expression and distribution of the interleukin-2 receptor (IL-2R) on leukocyte surface membranes was imaged using colloidal gold-labeled antibodies (i.e., immunogold). When compared with current SEM preparation procedures using CPD, Peldri II is a useful alternative that is thought to offer several important advantages.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060140403
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 51
    Electronic Resource
    Electronic Resource
    Three-dimensional observation of the fibroblast-like cells associated with the rat myenteric plexus, with special reference to the interstitial cells of Cajal (1989)
    Springer
    Cell & tissue research 255 (1989), S. 343-351 
    Details
    ISSN: 1432-0878
    Keywords: SEM ; TEM ; Interstitial cell ; Myenteric plexus ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary An extensive cellular network becomes visible over the myenteric plexus of the rat after removal of the overlying tissues under the scanning electron microscope. The cells are mainly stellate and have many slender processes via which they interconnect. They form a three-dimensional network and are closely associated with the ganglia and nerve bundles, and also extend over the smooth muscle cells. They are considered to correspond to the interstitial cells of Cajal because of their peculiar arrangement and their topography. Transmission electron-microscopic evidence demonstrates that the majority of those cells have features of fibroblasts. Gap junctions and intermediate junctions are observed between these fibroblast-like cells, and also between them and smooth muscle cells. Examination of serial thin sections reveals that single fibroblast-like interstitial cells connect to both circular and longitudinal muscle cells via gap junctions. It is suggested that the network of interstitial cells conducts electrical signals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224117
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 52
    Electronic Resource
    Electronic Resource
    Structure and superconductivity of Bi-(Pb)-Ca-Sr-Cu-O ceramics processed by arc melting (1989)
    Springer
    Journal of superconductivity 2 (1989), S. 501-511 
    Details
    ISSN: 1572-9605
    Keywords: superconductors ; Bi-(Pb)-Ca-Sr-Cu-O ; X-ray diffraction ; electrical resistivity ; critical current density ; SEM ; a.c. susceptibility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology , Physics
    Notes: Abstract The Bi-(Pb)-Ca-Sr-Cu-O ceramics of typical cation composition 2 (0.4) 223, presintered at 800°C, are formed by arc melting and rapidly cooling the 2021 superconducting phase, CaO, and Cu2O. The arc-melted samples sintered in air at 840°C exhibit a solid-state structural transformation of the components and a mixture of 2122 and 2223 superconducting phases, and small amounts of Ca2CuO3, Ca2PbO4, and CuO appear. When the arc melting is used as an intermediate stage in the preparation of the high-T c superconductors in this system, a significant increase in density (from 3.7 to 5.7 g/cm3) and in critical current density (from 28 to 60 A/cm2 in zero field and at liquid-nitrogen temperature) is observed, while the critical temperature remains practically unchanged (−104 K).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00627563
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 53
    Electronic Resource
    Electronic Resource
    Observation of GaAs - AlxGa1  -  x as heterostructures and quantum-well-wire structures using backscattered electron image (1989)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 12 (1989), S. 60-64 
    Details
    ISSN: 0741-0581
    Keywords: SEM ; Nondestructive observation ; Intermixing ; Fine pattern ; Focused ion beam ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: As a microscale tool for observing GaAs-Alx Ga1-xAs heterostructures, backscattered electron (BE) images in the scanning electron microscope (SEM) were compared with conventional secondary electron (SE) images. BE images were found to be more sensitive to compositional differences between GaAs and AlxGa1-xAs and less sensitive to surface roughness. BE images have a spatial resolution of 10 nm or better. This method enables the nondestructive observation of ultrafine lateral periodic structures, such as quantum-well-wire (QWW) structures, fabricated by compositional disordering technology using focused Ga ion-beam (Ga-FIB) implantation into GaAs-AlxGa1-xAs material.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060120108
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 54
    Electronic Resource
    Electronic Resource
    Observation of microdiffraction patterns with a dedicated STEM instrument (1989)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 11 (1989), S. 143-154 
    Details
    ISSN: 0741-0581
    Keywords: Detector systems for microdiffraction ; STEM imaging ; Coherent diffraction effects ; Image reconstruction from diffraction patterns ; EELS ; SEM ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: A two-dimensional detector system, designed for the observation and recording of microdiffraction patterns formed in an HB 5 scanning transmission electron microscope (STEM) is described and discussed. Possibilities are described and demonstrated for the simultaneous or successive recording of microdiffraction patterns from regions of diameter 3 å or more, bright- or dark-field STEM images, EELS spectra, secondary electron images, and in-line holograms. Applications of the system have been made to studies of catalyst particles, reflection-mode imaging of bulk surfaces, and image reconstruction from microdiffraction patterns obtained from each point of a STEM image.
    Additional Material: 9 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060110209
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 55
    Electronic Resource
    Electronic Resource
    Direct observation of immunoreactive sites and antibody molecules by ultrahigh-resolution scanning electron microscope (1989)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 12 (1989), S. 155-159 
    Details
    ISSN: 0741-0581
    Keywords: Antibody ; Ultrahigh-resolution SEM ; Colloidal gold ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Using an ultrahigh-resolution scanning electron microscope (SEM), an attempt was made to directly visualize antibody molecules at antigenic sites in rat pancreas or on silicone plates. Although individual antibody molecules could not be discerned, a fluffy meshwork, probably indicating several molecules, was seen. With further improvements in specimen preparation, the high-resolution SEM promises to be an important tool in examining individual antibody-antigen sites without the need of an electron-dense label such as colloidal gold.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060120209
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 56
    Electronic Resource
    Electronic Resource
    Application of an ultrahigh-resolution scanning electron microscope (UHS-T1) to biological specimens (1989)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 12 (1989), S. 146-154 
    Details
    ISSN: 0741-0581
    Keywords: SEM ; Intracellular structures ; Bacteriophage ; Ferritin ; IgG ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: In 1985 we developed an ultrahigh-resolution scanning electron microscope with a resolution of 0.5 nm. It is equipped with a field emission gun and an objective lens with a very short focal length. In this study we report a survey of some different preparation techniques and biological specimens using the new scanning electron microscope.Intracellular structures such as cell organelles were observed surprisingly sharper than those observed by ordinary scanning electron microscopes. However, at magnifications over 250,000 X, platinum particles could be discerned as scattered pebbles on the surface of all structures in coated materials. Using an uncoated but conductively stained specimen, we successfully observed ribosomes on a rough endoplasmic reticulum at a direct magnification of 1 million. In these images some protrusions were recognized on the ribosomes.Ferritin and immunoglobulin G were used as samples of biological macromolecules. These samples were observed without metal coating and conductive staining. The ferritin particles appeared as rounded bodies without any substructure on the surface and immunoglobulin G as complexes of three-unit bodies. In the latter the central body might correspond to the Fc fragment and two side ones to Fab fragments.We assume that ultrahigh-resolution scanning electron microscopy is an effective means for observation of the cell fine structures and biological macromolecules. It will open a new research field in biomedicine.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060120208
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 57
    Electronic Resource
    Electronic Resource
    Freeze-fracture cytochemistry: Review of methods (1989)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 13 (1989), S. 175-203 
    Details
    ISSN: 0741-0581
    Keywords: Colloidal gold ; Replica ; Membrane structure ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: “Freeze-fracture cytochemistry” encompasses a diversity of recently developed techniques in which freeze-fracture and cytochemistry are combined. Cytochemical labeling may, in principle, be integrated into one of three basic points in the standard freeze-fracture procedure; (1) before the specimen is frozen, (2) after it has been fractured, or (3) after it has been platinum shadowed and/or carbon coated. Visualization of the labeled cellular structures can be achieved by a variety of different methodologies. For example, the markers (usually colloidal gold particles) may be viewed embedded within a replica, or attached to it via fragments of membrane (or other cellular components). Sectioning is a central strategy in a number of techniques, either in combination with or in place of replication. The different combinations of methods that have been devised are not, for the most part, alternative ways of arriving at the same result; each provides quite distinct information about specific classes of membrane component or other structure in the cell. The purpose of this review is to present, within a single article, a systematic survey of the full range of techniques currently available in freeze-fracture cytochemistry. Emphasis is placed on explaining the principles underlying the methods and on illustrating their applications. With the success recently achieved, freeze-fracture cytochemistry has moved from the phase of experimental development to a position in which it may be expected increasingly to make significant contributions across a wide spectrum of problems in cell and membrane biology.
    Additional Material: 20 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060130306
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 58
    Electronic Resource
    Electronic Resource
    Composite replicas: Methodologies for direct evaluation of the relationship between intramembrane and extramembrane structures (1989)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 13 (1989), S. 216-227 
    Details
    ISSN: 0741-0581
    Keywords: Toad bladder ; Label fracture ; Colloidal gold ; Replica ; Membrane ; Freezing ; Glycocalyx ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Electron microscopic studies of membrane structure have been facilitated by the recent development of the composite replica technique in which the membrane is freeze-fractured, then inverted and the surface deep-etched and replicated. Examination in stereo of this composite preparation of two replicas with interposed half-membrane and associated surface elements reveals the physical relationship between structures on the surface and within the membrane. Composite replicas of the toad urinary bladder surface demonstrated connections of filamentous glycocalyx elements to intramembrane particles (IMPs). Using a bidirectional shadowing technique, many membrane surface particles also are shown to be associated with underlying IMPs, suggesting that these membrane surface particles are projections of the IMPs above the surface of the membrane. There is evidence that elements whose attachment sites relate to the half-membrane fractured away can be displaced from the membrane surface and lost. Labelling studies using colloidal gold-labelled antibodies were carried out to assess loss of surface mesh from fractured membrane. Gold distributions and amounts were similar in labelled surface replicas, label-fracture specimens, and labelled composite replicas, yet the amount of mesh detected in the composite replicas was less than in the surface replicas. This suggests that while some unlabelled or lightly labelled surface elements can be lost from fractured membranes, ligands stabilize elements and reduce their loss apparently by cross-linking them.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060130308
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 59
    Electronic Resource
    Electronic Resource
    Morphological studies on endocytosis of chondroitin sulphate proteoglycan by rat liver endothelial cells (1988)
    Springer
    Cell & tissue research 253 (1988), S. 39-45 
    Details
    ISSN: 1432-0878
    Keywords: Receptor-mediated endocytosis ; Chondroitin sulphate ; Colloidal gold ; Liver endothelial cells ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Endocytosis via the hyaluronic acid/chondroitin sulphate receptor of rat liver endothelial cells was studied ultrastructurally, by use of a probe consisting of chondroitin sulphate proteoglycan attached to 15-nm gold particles. The probe bound to the surface of the cells exclusively in coated regions of the plasma membrane. Internalization at 37° C took place in less than one minute during which time interval the bound probe was transferred to coated vesicles. Further transfer to lysosomes was delayed in association with an accumulation of probes in a prelysosomal compartment consisting of large vacuoles in which probes lined the inner aspect of the membrane. Transport to lysosomes occurred only after a lag phase of at least 40–60 min at 37° C.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00221737
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 60
    Electronic Resource
    Electronic Resource
    Cell to cell recognition between the ciliatePseudomicrothorax dubius and its food organisms: the role of surface charges (1988)
    Springer
    Protoplasma 143 (1988), S. 93-100 
    Details
    ISSN: 1615-6102
    Keywords: Ciliate ; Colloidal gold ; Cyanobacteria ; Phagocytic recognition ; Surface charges
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The importance of charged groups during phagocytic recognition of filamentous Cyanobacteria (Oscillatoria formosa andAnabaena spp.) by the stenophagic ciliatePseudomicrothorax dubius has been studied. Anionic and cationic domains are evenly and randomly distributed over the cyanobacterial surface, as demonstrated with scanning electron microscopy following labeling with colloidal gold (−) and colloidal gold coupled with poly-L-lysine (+). The phagocytosis ofOscillatoria was inhibited when filaments were treated with cationic reagents such as poly-L-lysine (pLL), FeCl3 and carbodiimide. In contrast elimination of cationic charges on theOscillatoria surface by treatment with poly-L-glutamic acid (pLGa) or colloidal gold did not affect phagocytosis. The effects of sequential treatment with pLL and pLGa demonstrated that pLL reduced phagocytosis of pLGa-pretreatedOscillatoria, whereas the pLGa restored phagocytosis of pLL-pretreated filaments. Scanning electron microscopy showed that pLL- or pLGa- treated filaments can still adsorb the oppositely charged colloidal gold particles on their surface. However, the treatment of filaments with pLL followed by pLGa prevented subsequent labeling with gold as well as with pLL-gold particles. Filaments ofAnabaena spp., which are not normally ingested byPseudomicrothorax, were also treated individually or sequentially with pLL and pLGa. None of these treatments, however, provoked phagocytosis ofAnabaena byPseudomicrothorax. We suggest that the surface charge alone does not play a crucial role in phagocytic recognition inPseudomicrothorax and that phagocytosis-specific molecules are implicated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01291153
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 61
    Electronic Resource
    Electronic Resource
    Interfacial phenomena and microstructural connectivity in explosively fabricated Y-Ba-Cu-O superconductors (1988)
    Springer
    Journal of superconductivity 1 (1988), S. 3-19 
    Details
    ISSN: 1572-9605
    Keywords: YBa2Cu3O7 ; explosive consolidation ; SEM ; TEM
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology , Physics
    Notes: Abstract Measurements of supercurrent transport and current density in explosively fabricated superconducting prototypes have been erratic, with current densities measured to be in excess of 103 A/cm2. This erratic behavior appears to be related to the degree of consolidation achieved for Y-Ba-Cu-O powders and, more specifically, to the connectivity or interfacial structure at individual, contacting, superconducting particles. We have used SEM and TEM to characterize and illustrate some of these features in preliminary superconducting prototypes composed of Y-Ba-Cu-O powder channels consolidated and encapsulated in copper and aluminum matrices by explosively generated shock waves. Observations of grain boundaries and interface phase regions cast some light on the prospects for producing good connectivity through optimizing the consolidation process and process parameters as well as the elimination of surface reactions on particles prior to consolidation, or thermal effects during consolidation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00617947
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 62
    Electronic Resource
    Electronic Resource
    Enzyme-gold affinity labelling of cellulose (1988)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 8 (1988), S. 371-379 
    Details
    ISSN: 0741-0581
    Keywords: Casuarina ; Cellulase ; Cell walls ; Codium ; Colloidal gold ; Dictyostelium ; Udotea ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: The enzyme-linked colloidal gold affinity labelling technique was tested as a method to localize cellulose on thin sections of plant cell walls and slime mold spores. Commercially available cellulase from cultures of Trichoderma reesei, the main components being cellobiohydrolase I and II (CBH I, CBH II) and endoglucanase (EG), was linked to colloidal gold by using standard techniques and applied as a dilute, buffered suspension to thin sections. After brief exposure, e.g., 15-30 minutes, cellulose exposed on the surface of sections was labelled with the enzyme-gold complex. Poststaining did not appear to have a deleterious effect on the labelled sections. The specificity of labelling was demonstrated by its complete inhibition when carboxymethylcellulose was incorporated in the labelling mixture, by lack of labelling of 1,4-β-mannans or 1,3-β-xylans in noncellulosic walls of marine algae, by lack of labelling of 1,4-β-glucans in chitin, by much lower labelling density when done at 4°C, and by lack of labelling when sections were predigested with cellulase. Labelling with the crude commercial cellulase was compared to labelling with purified CBH I-, CBH II-, and EG-linked colloidal gold, and the labelling pattern was similar. This method was found useful on conventionally fixed material and required no special preparation other than the use of inert (Ni or Au) grids and 0.5% gelatin to reduce nonspecific binding of the gold complex. Labelling was similar in the several embedding resins tested: LR White, Lowicryl K4M, Epon 812, and Spurr's. The cellulase-gold probe remained active for at least 4 weeks at 4°C and much longer when frozen at -80°C in 20% glycerol. This technique should prove useful in studies of cellulose degradation and cellulose deposition and of the interaction of cellulose with other wall components.
    Additional Material: 17 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060080406
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 63
    Electronic Resource
    Electronic Resource
    Immunocytochemical localization of methyl-coenzyme M reductase in Methanobacterium thermoautotrophicum (1987)
    Springer
    Archives of microbiology 147 (1987), S. 190-194 
    Details
    ISSN: 1432-072X
    Keywords: Methanobacterium thermoautotrophicum ; Methyl-CoM reductase ; Immunocytochemistry ; Colloidal gold ; Energy conservation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cells of Methanobacterium thermoautotrophicum were fixed with glutaraldehyde, sectioned and labeled with antibodies against the β subunit of component C (=methyl-CoM reductase) of methyl-CoM reductase system and with colloidal gold-labeled protein A. It was found that the gold particles were located predominantly in the vicinity of the cytoplasmic membrane, when the cells were grown under conditions where methyl-CoM reductase was not overproduced. This finding confirms the recent data obtained with Methanococcus voltae showing via the same immunocytochemical localization technique that in this organism methyl-CoM reductase is membrane associated.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00415283
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 64
    Electronic Resource
    Electronic Resource
    A comparison of wheat germ agglutinin binding between normal and sickle red blood cells (1987)
    Springer
    Cell & tissue research 248 (1987), S. 267-273 
    Details
    ISSN: 1432-0878
    Keywords: Sickle cells ; Wheat germ agglutinin ; Glycophorin A ; Membranes ; Colloidal gold ; Human
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Using the label-fracture technique, an ultrastructural comparison was made of the number and distribution of wheat germ agglutinin (WGA)-binding sites between human normal and sickle red blood cells. The WGA was adsorbed to colloidal gold, and quantitative analysis of the electron micrographs revealed that more binding sites were present on the sickle erythrocytes than on the normal erythrocytes. Moreover, the sites were more clustered on the sickle red cells than on the normal red cells. Use of another lectin, Bandieraea simplicifolia-II, revealed that it did not bind to normal or sickle red cells. Because of the affinity of the WGA for sialic acid residues, it is probable that the WGA is binding to a transmembrane sialoglycoprotein, glycophorin A. The conformation and/or distribution of the glycophorin A molecules may be altered by the sickle hemoglobin that binds to the red cell membrane. Hence, as detected by WGA, new surface receptors, which could play a role in the adhesion of sickle cells to endothelium may be exposed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00218193
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 65
    Electronic Resource
    Electronic Resource
    The ash wilt disease: A preliminary investigation of wood anatomy (1987)
    Springer
    European journal of plant pathology 93 (1987), S. 253-260 
    Details
    ISSN: 1573-8469
    Keywords: Dieback ; Fraxinus excelsior ; hyphae ; tyloses ; parenchymatous cells ; SEM
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Samenvatting Houtmonsters van zieke essen werden macroscopisch, microscopisch en met de SEM onderzocht om de oorzaak van de ‘esseverwelkingsziekte’ op te sporen. Deze ziekte, ook wel aangeduid met ‘essensterven’, vertoont grote overeenkomsten met de in de Verenigde Staten van Amerika en in Groot-Brittanië al langer bekende ‘ash dieback’. De gevonden verschijnselen, in het bijzonder de verstopping van vaten door thyllen en het vóórkomen van schimmeldraden in een van de monsters, vertonen veel overeenkomsten met karakteristieke symptomen van de iepeziekte en van de eikeverwelking.
    Notes: Abstract A macroscopical, microscopical and SEM examination of wood samples, taken from diseased ash trees, was undertaken to probe into the cause of an ash wilt disease considered to be identical with dieback. The symptoms, particularly the obstruction of the vessels by tyloses and the occurrence of fungal hyphae in one specimen, bear a strong resemblance to those found in Dutch elm disease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01998200
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 66
    Electronic Resource
    Electronic Resource
    Comparison of Two Digestive Techniques for Preparation of Vascular Elastic Networks for SEM Observation (1987)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 6 (1987), S. 335-348 
    Details
    ISSN: 0741-0581
    Keywords: Digestive techniques ; Vascular elastic networks ; SEM ; Blood vessels ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Two digestion procedures are now available to expose and isolate networks of vascular elastic fibers for three-dimensional SEM observation. This study was designed to observe and elucidate the differences between the two types of digestion (sodium hydroxide vs. formic acid) and the differences between the two types of dehydration (ethanol-critical-point drying vs. freeze drying) used in each procedure. Canine venous valve segments, containing delicate networks of elastic fibers, and femoral arteries, containing large elastic lamellae, were used to compare the effects of the digestion and dehydration procedures on two types of vessels with different content and organization of elastic tissue. Results indicated there was no significant difference in the architecture of the elastic networks of either vessel based on the method of digestion. The major architectural changes in the elastic networks occurred as a result of the dehydration procedure used following digestion. Freeze drying is probably the best for arterial specimens due to their prominent lamellae, which give added support to maintain their normal architecture. It is suggested that both methods of dehydration be used on corresponding venous specimens containing delicate elastic networks. In this way, the investigator can benefit from the advantages of each method and overcome their respective disadvantages to get a more accurate picture of the three-dimensional architecture of these delicate networks.
    Additional Material: 17 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060060404
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 67
    Electronic Resource
    Electronic Resource
    Postreplication labeling of E-leaflet molecules: Membrane immunoglobulins localized in sectioned, labeled replicas examined by TEM and HVEM (1987)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 7 (1987), S. 1-16 
    Details
    ISSN: 0741-0581
    Keywords: External determinants ; Colloidal gold ; High-voltage electron microscopy ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Conventional freeze-fracture techniques were combined with immunogold labeling and with plastic embedding and sectioning to analyze the distribution of membrane immunoglobulins (mIgs) and their associated intramembrane particles (IMPs) in E-face replicas of murine B-lymphocyte plasma membranes. Immunogold labels were applied to cells after the process of freeze-fracture and replication. Conventional stereoscopic transmission electron microscopic examination of sectioned, labeled replicas (SLRs) revealed that the gold-labeled mIgs were bound to and localized on the outer leaflets of split and replicated membranes. The gold labels were attached to the external determinants of the mIg molecules, which were retained beneath and contiguous with the replicated E-faces. The mIgs were also localized on the external surface of unreplicated microvilli. In addition, thick sections examined by high-voltage transmission electron microscopy (HVEM) revealed large expanses of replica with well-resolved IMPs. mIgs colocalized with small-diameter (〈60 Å) IMPs in E-face replicas of B-lymphocytes whose mIgs were patched by anti-immunoglobulin. Thus, postreplication E-surface labeling of split and replicated membranes is a high-resolution technique that is suitable for the study of membrane protein distribution in E-face replicas and contiguous nonreplicated tissue.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060070102
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 68
    Electronic Resource
    Electronic Resource
    Postembedding ultrastructural immunocytochemistry of neural antigens on tissues previously processed for routine light and electron microscopy: Preservation of antigenicity up to 22 years after initial fixation (1987)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 5 (1987), S. 81-90 
    Details
    ISSN: 0741-0581
    Keywords: Immunocytochemistry ; Electron microscopy ; Colloidal gold ; Glial fibrillary acidic protein ; Neuron-specific enolase ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: A postembedding immunocytochemical technique is described that allows ultrastructural localization of glial fibrillary acidic protein and neuron-specific enolase on tissues originally processed only for routine light and electron microscopy. Use of the oxidizing agent sodium metaperiodate prior to incubation with the primary antiserum sufficiently removes osmium tetroxide (OsO4) from potential antigen - antibody combining sites to allow specific localization of these neural antigens by colloidal gold immunolabelling. Both human and monkey neural tissues, prepared for routine ultrastructural examination with aldehyde fixatives and OsO4 postfixation, show excellent ultrastructural morphology and antigen localization. In addition, formalin-fixed, paraffin-embedded pathological human brain tissues, obtained at autopsy up to 22 years previously, show good ultrastructural immunolocalization of glial fibrillary acidic protein when re-embedded for electron microscopy. Thus, ultrastructural immunolocalization of certain neural antigens is easily achieved in tissues originally processed for routine light and electron microscopy. This allows re-examination of archival tissues using current immunocy-tochemical advances, including that of selected pathological tissues previously prepared solely for light microscopy.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060050109
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 69
    Electronic Resource
    Electronic Resource
    Introduction of the protein G - gold complex for high-resolution immunocytochemistry (1987)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 6 (1987), S. 7-13 
    Details
    ISSN: 0741-0581
    Keywords: Protein G ; Colloidal gold ; Protein G-gold ; Immuocyto-chemistry ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Protein G, an immunoglobulin G-binding molecule, was tagged with colloidal gold particles and was applied in immunocytochemistry. The present study reports the conditions required for the preparation of the protein G-gold complex and for its application in electron microscopy. Once used in combination with specific primary antibodies, the protein G-gold complex was able to yield highly specific labelings over particular tissue compartments. Various control experiments confirmed the specificity of the labelings obtained. Since the IgG-binding properties of protein G are superior to those displayed by protein A, the protein G-gold appears a far better tool for high-resolution immunocytochemistry.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060060103
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 70
    Electronic Resource
    Electronic Resource
    Steedman's polyester wax embedment and de-embedment for combined light and scanning electron microscopy (1987)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 6 (1987), S. 35-41 
    Details
    ISSN: 0741-0581
    Keywords: Technique ; SEM ; Histology ; Polyester wax ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: Steedman's polyester wax mixture is a good, general-purpose histological embedding medium that is suitable and convenient to use when it is desirable to combine light microscopy with scanning electron microscopy (SEM). A range of properties recommend this wax: it has a low melting temperature (37°C), is readily soluble in most dehydrating agents, results in negligible tissue shrinkage, preserves tissue antigenicity, and may even be used as a solvent for fixative agents. We prepare and embed tissues in polyester for light microscopy much as they would be for paraffin wax. For SEM, the block surface is micro- or ultraplaned, utilizing, respectively, a standard rotary microtome with razor blade knives or an ultramicrotome with glass knives. The block is de-waxed in absolute alcohol and then taken to critical point drying. Similarly, sections mounted on coverslips or glass slides may be brought to the SEM after removing the wax. This enables one to bring to the SEM relatively large block faces or sections with good control over orientation. We find the results to be superior to similar procedures employing paraffin. We believe it to be more versatile and equivalent or superior to a variety of other techniques designed to gain access to the interior of tissues with SEM.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060060106
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 71
    Electronic Resource
    Electronic Resource
    Chemoreceptors of adult Mexican bean beetles: External morphology and role in food preference (1986)
    Springer
    Entomologia experimentalis et applicata 40 (1986), S. 3-12 
    Details
    ISSN: 1570-7458
    Keywords: Mexican bean beetle ; Epilachna varivestis ; host selection ; food preference ; chemoreceptors ; SEM ; host plant resistance ; ablation ; olfaction ; taste
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé E. varivestis Mulsant, insecte oligophage, accepte les feuilles de Glycine max (L.) Merrill (soja) comme aliment, mais présente des préférences marquées parmi les variétés. On considère que l'origine de la discrimination entre le cultivar sensible ‘Williams’ et PI 229358 résistant, est chimique. Des expériences de choix avec des rondelles de feuilles, après ablation des sensilles chimiques des antennes et des palpes maxillaires et labiaux, et des tarses antérieurs, ont montré que les sensilles chimiques étaient presque entièrement responsables de la discrimination entre les feuillages de génotypes résistant et sensible. Tous les appendices ont été observés au microscope à balayage, et, à l'exception des tarses antérieurs, ils portaient plusieurs types de sensilles gustatives et olfactives, qui ont été représentées et décrites. Puisque les observations comportementales montrent que l'olfaction est normalement suffisante pour provoquer le rejet de PI 229358, nous concluons que les sensilles olfactives des antennes et des palpes maxillaires jouent un rôle crucial dans le choix de l'aliment.
    Notes: Abstract The Mexican bean beetle, Epilachna varivestis Mulsant (Coleoptera; Coccinellidae), is an oligophagous insect that accepts leaves of soybean, Glycine max (L.) Merrill, as food but exhibits distinct preferences among varieties. It is believed that the basis for discrimination between the resistant PI 229358 and the susceptible cv. ‘Williams’ is chemical. By the ablation of chemosensilla on antennae, maxillary palpi, labial palpi, and foretarsi, followed with leaf disc preference tests, we found antennal and maxillary palp chemosensilla to be almost entirely responsible for Mexican bean beetles' discrimination between foliage of the resistant and susceptible genotypes. All appendages, and the epipharynx, were observed by scanning electron microscopy and, except for foretarsi, were found to bear several types of presumably olfactory and gustatory sensilla, which are figured and described. Since behavioral observations show that olfaction is usually sufficient to cause rejection of PI 229358, we conclude that olfactory sensilla mainly on antennae and maxillary palpi are critical in food discrimination in this system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00187017
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 72
    Electronic Resource
    Electronic Resource
    Fenestrations of the basal lamina of intestinal villi of the rat (1985)
    Springer
    Cell & tissue research 239 (1985), S. 183-188 
    Details
    ISSN: 1432-0878
    Keywords: Fenestrations ; Basal lamina ; Intestine, small ; Immune system ; SEM ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Fenestrations of the basal lamina of rat intestinal villi were revealed by scanning electron microscopy after removal of the overlying epithelial cells by osmic acid maceration. These fenestrations are circular to oval in shape and are 0.5 μm to 5 μm in diameter. They are richly distributed at a density of 1–2x104/mm2 in the upper two thirds of the villi, except at the very tips. Roughly 500 fenestrations are found on each side of an average sized tongue-shaped villus. Transmission electron-microscopic observations showed that these fenestrations were passages for migrating cells of the immune system such as lymphocytes, eosinophils and macrophages. Protrusions from the basal parts of epithelial cells were also observed passing through these fenestrations. These findings are discussed with respect to their immunological implications and to the passage of nutrients.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00214918
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 73
    Electronic Resource
    Electronic Resource
    Quadriflagellar primary spermatocytes in the cotton leafworm, Spodoptera littoralis (Boisd.) (Noctuidae, Lepidoptera) (1985)
    Springer
    Cell & tissue research 242 (1985), S. 681-683 
    Details
    ISSN: 1432-0878
    Keywords: Spermatocyte ; Flagella ; SEM ; Lepidoptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The surface morphology of primary spermatocytes from testicular cysts of the last instar larvae of Spodoptera littoralis was examined by scanning electron microscopy (SEM). At this stage, each primary spermatocyte possesses four developing flagella, directed towards the lumen of the cyst. The identical length of flagella in all primary spermatocytes from a single cyst indicates that the initiation and rate of flagellar growth are synchronized. Some asynchrony can, however, be observed in the translocation of flagella to secondary spermatocytes during the first meiotic division.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225438
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 74
    Electronic Resource
    Electronic Resource
    An application of rapid freezing, freeze substitution-fixation for scanning electron microscopy (1985)
    New York, NY : Wiley-Blackwell
    Journal of Electron Microscopy Technique 2 (1985), S. 201-208 
    Details
    ISSN: 0741-0581
    Keywords: SEM ; Rapid freezing ; Osmium ; Dimethyl sulfoxide ; Intracellular structure ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Natural Sciences in General
    Notes: A combined technique of the rapid freezing, freeze substitution-fixation method and the osmium-DMSO-osmium method was devised. By this combined method we clearly observed the architecture of intracellular components in three dimensions. Morphological characteristics were generally similar to those of tissue prepared by the osmium-DMSO-osmium method but different in some respects. Mucigen droplets in intestinal goblet cells, for example, appeared as separated spheres, while in specimens prepared by chemical fixation they were observed as a mass of fused droplets. In the Golgi complex, all cisternae were extremely flat, although they usually dilated on the cis side after chemical fixation. Particles on the mitochondrial tubules of liver cells were well distinguished. They were mushroom shaped, as are those observed by negative staining. The combined method, that is, the rapid freezing, osmium-DMSO-osmium method, is thought to be effective for studying the true structure of intracellular components by scanning electron microscopy.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jemt.1060020305
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...