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  • Articles  (137)
  • Triticum aestivum  (132)
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  • Springer  (137)
  • 1995-1999  (137)
  • Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition  (137)
  • 1
    ISSN: 1432-0789
    Keywords: 15N-labelled fertilizer ; Added N interaction Fertilizer N uptake ; Soil N uptake ; Wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effects of 15N-labelled ammonium nitrate, urea and ammonium sulphate on yield and uptake of labelled and unlabelled N by wheat (Triticum aestivum L. cv. Mexi-Pak-65) were studied in a field experiment. The dry matter and N yields were significantly increased with fertilizer N application compared to those from unfertilized soil. The wheat crop used 64.0–74.8%, 61.5–64.7% and 61.7–63.4% of the N from ammonium nitrate, urea and ammonium sulphate, respectively. The fertilizer N uptake showed that ammonium nitrate was a more available source of N for wheat than urea and ammonium sulphate. The effective use of fertilizer N (ratio of fertilizer N in grain to fertilizer N in whole plant) was statistically similar for the three N fertilizers. The application of fertilizer N increased the uptake of unlabelled soil N by wheat, a result attributed to a positive added N interaction, which varied with the method of application of fertilizer N. Ammonium nitrate, urea and ammonium sulphate gave 59.3%, 42.8% and 26.3% more added N interaction, respectively, when applied by the broadcast/worked-in method than with band placement. A highly significant correlation between soil N and grain yield, dry matter and added N interaction showed that soil N was more important than fertilizer N in wheat production. A values were not significantly correlated with added N interaction (r=0.719). The observed added N interaction may have been the result of pool substitution, whereby added labelled fertilizer N stood proxy for unlabelled soil N.
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  • 2
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    Biology and fertility of soils 23 (1996), S. 327-331 
    ISSN: 1432-0789
    Keywords: CO2 emission ; Field method ; Soil respiration ; Triticum aestivum ; Soil moisture ; Carbon reservoirs ; Greenhouse effect ; Grey forest soil ; Mineralization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The dynamics of the rate of CO2 evolution from soil in fallow and croplant under spring wheat (Triticum aestivum L.) was studied in a crop rotation in grey forest soil of the Baikal forest-steppe during the growing season and in different years. It was shown that the regional characteristics of soils and hydrothermal conditions in different years affect the rate of CO2 evolution in agroecosystems. The seasonal dynamics of CO2 is characterized by insignificant changes in the autumn to spring period and enhanced emission in hot and dry summers. CO2 evolution is assumed to increase due to enhanced mineralization and partial diffusion from the carbonate horizon at the depth of the seasonal frost. During the growing season the dynamics of CO2 evolution depends on the soil moisture regime. There was a strong correlation between the rate of CO2 emission and soil moisture in the particularly dry year of 1993 (η=0.86) and a moderate correlation in the other years (η=0.38–0.54). The effect of the previous crop and fertilizer application on the rate of CO2 emission was insignificant. In a continuous fallow the total carbon release into the atmosphere varied throughout the years studied from 558 to 1880 kg ha-1. Humus losses varied from 0.9% to 3.1%.
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  • 3
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    Biology and fertility of soils 23 (1996), S. 327-331 
    ISSN: 1432-0789
    Keywords: Key words CO2 emission ; Field method ; Soil respiration ; Triticum aestivum ; Soil moisture ; Carbon reservoirs ; Greenhouse effect ; Grey forest soil ; Mineralization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The dynamics of the rate of CO2 evolution from soil in fallow and cropland under spring wheat (Triticum aestivum L.) was studied in a crop rotation in grey forest soil of the Baikal forest-steppe during the growing season and in different years. It was shown that the regional characteristics of soils and hydrothermal conditions in different years affect the rate of CO2 evolution in agroecosystems. The seasonal dynamics of CO2 is characterized by insignificant changes in the autumn to spring period and enhanced emission in hot and dry summers. CO2 evolution is assumed to increase due to enhanced mineralization and partial diffusion from the carbonate horizon at the depth of the seasonal frost. During the growing season the dynamics of CO2 evolution depends on the soil moisture regime. There was a strong correlation between the rate of CO2 emission and soil moisture in the particularly dry year of 1993 (η=0.86) and a moderate correlation in the other years (η=0.38–0.54). The effect of the previous crop and fertilizer application on the rate of CO2 emission was insignificant. In a continuous fallow the total carbon release into the atmosphere varied throughout the years studied from 558 to 1880 kg ha–1. Humus losses varied from 0.9% to 3.1%.
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  • 4
    ISSN: 1432-0789
    Keywords: Key words Phosphate-solubilizing microorganisms ; Mussoorie rock phosphate ; Vesicular-arbuscular mycorrhizae ; Triticum aestivum ; Nutrient-deficient soils
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  The effect of inoculating wheat (Triticum aestivum L.) with the PO4 3–-solubilizing microorganisms (PSM) Bacillus circulans and Cladosporium herbarum and the vesicular-arbuscular mycorrhizal (VAM) fungus Glomus sp. 88 with or without Mussoorie rock phosphate (MRP) amendment in a nutrient-deficient natural sandy soil was studied. In the sandy soil of low fertility root colonization by VAM fungi was low. Inoculation with Glomus sp. 88 improved root colonization. At maturity, grain and straw yields as well as N and P uptake improved significantly following inoculation with PSM or the VAM fungus. These increases were higher on combined inoculation of PSM and the VAM fungus with MRP amendment. In general, a larger population of PSM was maintained in the rhizosphere of wheat in treatments with VAM fungal inoculation and MRP amendment. The results suggest that combined inoculation with PSM and a VAM fungus along with MRP amendment can improve crop yields in nutrient-deficient soils.
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  • 5
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    Biology and fertility of soils 23 (1996), S. 273-281 
    ISSN: 1432-0789
    Keywords: Wheat ; Triticum aestivum ; Rhizosphere ; Soil microflora ; Gram-negative bacteria ; API 20 NE ; Flavobacterium spp ; Cytophaga
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We identified 161 Gram-negative bacterial strains isolated from the root surface of wheat grown under different soil conditions. The strains were divided into seven groups based on major morphological and physiological properties. Taxonomic allocation of the groups was verified by guanine+cytosine contents of DNA. Except for one group, which may be assumed to include bacteria belonging to the genera Flavobacterium and Cytophaga, the various groups were taxonomically united. The distribution of the groups changed with soil improvement. Pseudomonads predominated in unimproved soil, but Flavobacterium and Cytophaga spp. were predominant in the most improved soil. As all the strains were non-fermentative by Hugh and Leifson's test, API 20NE identification was applied. However, many strains were misidentified by this system, especially in the Flavobacterium and Cytophaga spp. group. For ecological studies, the strains were classified to species level by the API 20 NE system and by the results of a combination of guanine+cytosine (mol%) and isoprenoid quinone data. The pattern of distribution of the bacteria on the root surface of wheat varied at species level within one genus depending on soil conditions.
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  • 6
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    Nutrient cycling in agroecosystems 53 (1999), S. 157-175 
    ISSN: 1573-0867
    Keywords: Brassica napus ; Cicer arietinum ; current P ; Lens culinaris ; Lupinus albus ; Lupinus angustifolius ; P concentration response ; P content response ; Pisum sativum ; previous P ; sigmoid response ; single superphosphate ; Triticum aestivum ; Vicia faba ; yield response
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Phosphorus (P) is a major deficiency of soils of south-western Australia (WA). The fertilizer P requirements are not known for grain legumes being evaluated for neutral to alkaline, fine textured soils in WA. To rectify this, glasshouse and field experiments were undertaken to compare the responses of several grain legume species, wheat and canola to applications of single superphosphate and the results are reported in this paper. The glasshouse experiments measured responses of dried tops, harvested at 26 to 42 days after sowing, to P that was freshly-applied (current P) and previously-applied (previous P). Responses in the glasshouse were measured using yield, P concentration and P content (P concentration multiplied by yield) of oven dried tops of the following: wheat (Triticum aestivum), canola (Brassica napus), faba bean (Vicia faba), chickpea (Cicer arietinum), lentil (Lens culinaris), field pea (Pisum sativum), albus lupin (Lupinus albus) and narrow leaf lupin (Lupinus angustifolius). Field experiments in 1994 and 1995 compared seed (grain) yield responses of faba bean, chickpea, lentil, albus lupin and wheat to applications of current P. The P was banded (drilled) with the seed while sowing at 5 cm depth. Canola and wheat produced very large yield responses to increasing applications of current P. Responses were much smaller for albus lupin, faba bean and chickpea. Responses for lentil, narrow leaf lupin and field pea, fell in between responses of the small and large seeded species. Similar trends for responses were obtained as measured using yield, P concentration, or P content. For soils treated with previous P, similar trends were observed as for current P, but differences in yield responses between species were much less marked and the response curves tended to become more sigmoid. In the field experiments, grain yield responses to current P of albus lupin and chickpea were less than that for wheat. Relative to wheat, faba bean was the most responsive grain legume to applications of current P, with lentil producing similar responses to wheat in one experiment at a newly cleared, P deficient site.
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  • 7
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    Nutrient cycling in agroecosystems 44 (1995), S. 205-215 
    ISSN: 1573-0867
    Keywords: Incubation of phosphorus in soil ; relative effectiveness ; superphosphate ; ×Triticosecale ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Single superphosphate was incubated for six months at 25°C in soil which had been subject to one of three moisture treatments. These were: dried in a glasshouse, dried at a constant temperature of 25°C, or moist soil. Phosphorus (P) effectiveness was then compared with effectiveness of P from freshly-applied superphosphate using yields of wheat (Triticum aestivum) and triticale (×Triticosecale) tops in pot experiments. Incubation in soil which had been dried at 25°C did not decrease the effectiveness of the P. Incubation in moist soil decreased it to about 20% of the effectiveness of freshly-applied P in one case and to about 50% in the other case. Incubation in soil which had been dried in a glasshouse also decreased its effectiveness. The decrease varied with conditions, but in two cases the P was 70% as effective as freshly-applied P, and in one case only 45% as effective. Presumably sufficient moisture was present in the soil dried in the glasshouse to enable water-soluble P present in the fertilizer to react with the soil.
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  • 8
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    European journal of plant pathology 101 (1995), S. 613-622 
    ISSN: 1573-8469
    Keywords: epidemiology ; isozyme ; Triticum aestivum ; virulence ; wheat brown rust ; wheat leaf rust
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Wheat brown rust pathotype (pt) 104-2,3,(6),(7), 11 was first detected in Australasia in Victoria during 1984. Although it appeared similar to a pre-existing pathotype, 104-2,3,6,(7), detailed greenhouse test revealed nine pathogenic differences between the two rusts. Six differences involved contrasting virulence/avirulence for the resistance genes/specificitiesLr12, Lr27+Lr31 andLr16, and three uncharacterised genes, present in the wheat cultivars Gaza and Harrier, and in triticale cultivar Lasko. Differences in partial virulence between the pathotypes were found for the genesLr2a, Lr13 andLr26. A comparison of the phenotypes for 13 isozyme systems in the two pathotypes revealed two differences, including aPgm2 allele in pt 104-2,3,(6),(7),11 not found in other contemporary AustralasianPuccinia recondita f. sp.tritici pathotypes. On the basis of these differences, it was concluded that pt 104-2,3,(6),(7),11 was introduced into the Australasian region before or during 1984. Seven variants of pt 104-2,3,(6),(7),11, that differed by single virulences, were detected during 1984–1992. Pt 104-2,3,(6),(7),11 and a derivative pathotype with virulence forLr20 underwent rapid increases in frequency, largely displacing pathotypes which predominated before 1984. Although first detected in eastern Australia, both pathotypes spread to New Zealand, and the derivative pathotype appeared in Western Australia. The rapid spread and increase of these pathotypes could not be explained by host selection. Pt 104-2,3,(6),(7),11 and derivatives may therefore be more aggressive than other contemporary Australasian pathotypes.
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  • 9
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    Plant and soil 176 (1995), S. 1-6 
    ISSN: 1573-5036
    Keywords: growth stage ; plant uptake ; radiocaesium ; solution culture ; Triticum aestivum ; cv. Tonic ; spring wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Spring wheat plants were grown in a 137Cs labelled nutrient solution, either in the presence or absence of NH4 as a secondary N source. Between 11 and 64 days after sowing (DAS), plants were harvested on nine occasions. The plants supplied with NH4 and NO3 had lower root 137Cs Activity Concentrations (AC) than those supplied with NO3 only. Shoot AC were equal in both nutrition treatments. Shoot and root 137Cs AC (dry weight basis) showed the same trends with plant age in both nutrition treatments. Shoot AC almost doubled between 11 and 28 DAS after which they gradually decreased concomitant with a similar decrease in K concentrations. Root AC were always higher than shoot AC and increased to a maximum at 35 DAS after which they fluctuated. Expressed on a tissue water basis, the 137Cs AC varied less during plant age than did dry weight based AC. Furthermore, root and shoot AC expressed on a tissue water basis were almost equal. It is shown that the initial increase in 137Cs AC in both root and shoot can largely be explained by the initial dilution of absorbed 137Cs in the unlabelled seedling tissues. No correlation was found between K and 137Cs distribution among ears, leaves, stems and roots in 64 old wheat plants. NH4 as a secondary N source in a nitrate nutrient solution marginally affected 137Cs distribution.
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  • 10
    ISSN: 1573-5036
    Keywords: breeding ; chromosome ; copper ; copper-efficiency ; Triticum aestivum ; wheat-rye
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A greenhouse study was conducted to determine the Cu-efficiency of 5A/5RL wheat-rye translocation lines and nine wheat genotypes grown in a Cu-deficient orthic dark grey chernozemic soil. The soil used was characterized by pH 5.4 and 0.48 μg Cu g−1 soil (DTPA), and was obtained from a known Cu-deficient site at Stony Plain, Central Alberta. Twelve genotypes (three 5A/5RL wheat-rye translocation lines and nine wheat cultivars) and two Cu treatments (soil amended with 145 μg Cu kg−1 soil, and non-amended) were used. The efficiency for Cu use was evaluated for grain yield and yield components. 5A/5RL wheat-rye translocation lines individually and as a group showed significantly (p〈0.05) higher grain yield both in +Cu and −Cu treatments compared to wheat genotypes without the wheat-rye chromosome translocation. 5A/5RL wheat-rye translocation lines demonstrated Cu-efficiency ranging from 70–127% for grain yield. Only two wheat cultivars (Kenya Leopard and Columbus) showed comparable efficiency (70 and 76% respectively). Wheat cultivars Kwale, Kenya Tausi, Roblin, Katepwa, Park, Oslo and Biggar showed low Cu-efficiency (10–36%). The study confirms that the 5A/5RL wheat-rye translocation confers useful levels of Cu-efficiency to wheat, that can be used as an additional source of variability in breeding programs. ei]L V Kochian
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  • 11
    ISSN: 1573-5036
    Keywords: genotypic variation ; Secale cereale ; Triticum aestivum ; Triticum durum ; zinc efficiency ; zinc deficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Effect of zinc (Zn) nutritional status on uptake of inorganic 65Zn was studied in rye (Secale cereale, cv. Aslim), three bread wheat (Triticum aestivum, cvs. Dagdas, Bezostaja, BDME-10) and durum wheat (Triticum durum, cv. Kunduru-1149) cultivars grown for 13 days in nutrient solution under controlled environmental conditions. The cultivars were selected based on their response to Zn deficiency and to Zn fertilization in calcareous soils under field conditions. When grown in Zn-deficient calcareous soil in the field, the rye cultivar had the highest, and the durum wheat the lowest Zn efficiency. Among the bread wheats, BDME-10 showed higher susceptibility to Zn deficiency and Bezostaja and Dagdas were less affected by Zn deficiency. Similarly to field conditions, in nutrient solution visual Zn deficiency symptoms (i.e. necrotic lesions on leaf blade) appeared to be more severe in Kunduru-1149 and BDME-10 and less severe in rye cultivar Aslim. Under Zn deficiency, shoot concentrations of Zn were similar between all cultivars. Cultivars with adequate Zn supply did not differ in uptake and root-to-shoot translocation rate of 65Zn, but under Zn deficiency there were distinct differences; rye showed the highest rate of Zn uptake and the durum wheat the lowest. In the case of bread wheat cultivars, 65Zn uptake rate was about the same and not related to their differential Zn efficiency. Under Zn deficiency, rye had the highest rate of root-to-shoot translocation of 65Zn, while all bread and durum wheat cultivars were similar in their capacity to translocate 65Zn from roots to shoots. When Zn2+ activity in uptake solution ranged between 117 p M and 34550 pM, Zn-efficient and Zn-inefficient bread wheat genotypes were again similar in uptake and root-to-shoot translocation rate of 65Zn. The results indicate that high Zn efficiency of rye can be attributed to its greater Zn uptake capacity from soils. The inability of the durum wheat cultivar Kunduru-1149 to have a high Zn uptake capacity seems to be an important reason for its Zn inefficiency. Differential Zn efficiency between the bread wheat cultivars used in this study is not related to their capacity to take up inorganic Zn.
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  • 12
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    Euphytica 100 (1998), S. 189-196 
    ISSN: 1573-5060
    Keywords: cytoplasmic effects ; inheritance ; quality ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The inheritances of thousand kernel weight (TKW), protein percentage, protein quality and grain hardness were studied through an 11 x 11 complete diallel set of bread wheat genotypes consisting of four alloplasmic lines of Selkirk, two alloplasmic lines of Siete Cerros 66, and five commercial cultivars. Genetic components accounted for 93%, 90%, 78%, and 92% of total variation for TKW, protein percentage, protein quality, and grain hardness, respectively. General combining ability (GCA) effects were dominant for TKW (48% GCA, 38% SCA [specific combining ability], and 7% reciprocal effects [RE]), protein percentage (70% GCA, 10% SCA, and 10% RE), and grain hardness (59% GCA, 29% SCA, and 4% RE). However, SCA effects dominated for protein quality (30% GCA, 43% SCA, and 5% RE). Broad- and narrow-sense heritabilities were estimated at 0.95 and 0.65 for TKW, 0.94 and 0.82 for protein percentage, 0.83 and 0.47 for protein quality, and 0.95 and 0.74 for grain hardness. Reciprocal effects were highly significant for all quality traits, but less effective than additive and non-additive gene effects. Aegilops cylindrica, Ae. ventricosa, and Triticum turgidum cytoplasms showed positive effects on TKW in some crosses. Ae. cylindrica, Ae. variabilis, and Ae. uniaristata cytoplasms seemed to have potential for improving protein percentage. T. aestivum cytoplasms were superior to alien cytoplasms for protein quality. Bolal 2973, Kiraç 66 and Bezostaja 1 cytoplasms increased protein quality in some crosses. Ae. cylindrica, Ae. variabilis, Ae. ventricosa and Ae. uniaristata cytoplasms had significant effects on grain hardness. The cytoplasmic variation in B type T. aestivum cytoplasm was found to be significant for all traits.
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  • 13
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; combining ability ; heterosis ; genetic distance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In wheat, the possibility of introducing F1 seed into practical agriculture has been greatly enhanced by the discovery of effective chemical hybridising agents (CHAs). Although some technical and economic problems concerning the use of CHAs for large-scale production of F1 seed remain to be solved, a first group of F1 hybrids has been submitted for registration in several European countries i.e., France, England and Italy. Combining ability for grain yield and several agronomic and quality traits was studied in an eight-parent diallel cross. Highly significant combining ability effects were observed for all the traits while specific combining ability effects were statistically significant for grain yield, plant height, heading time and Chopin alveograph parameter P. The level of genetic diversity between parents as estimated using molecular markers is considered a tool for predicting the hybrid performance and heterosis of crosses. To explore this possibility, RFLP and RAPD markers were used to predict the performance of hybrids obtained from diallel and top crosses. The performance of the hybrids was determined in replicated plot trials sown at normal seed density in several locations. Coefficient of parentage (rp), based on pedigree information for all the pairwise combinations of the parents ranged from 0.01 to 0.34. The parents were assayed for random amplified polymorphic DNA (RAPD) with 87 primers which generated 304 polymorphic bands. Genetic similarity between parents, estimated on the basis of common bands using the Jaccard's similarity coefficient (J), ranged from 0.25 to 0.57. Correlation between parental diversity and hybrid performance was generally weak. A positive trend is observed in the yield potential of the hybrids produced in Italy in the last 10 years. In fact among the first set of hybrids produced by random crossing of the available cultivars, none produced 10% more than the checks whereas the last generation of hybrids includes combinations yielding 15% more than the best standards. Our results clearly indicate the need to develop specific strategies in order to identify and/or to select parental lines with a high level of general combining ability (GCA) and specific combining ability (SCA). The information regarding the genetic diversity of the parental lines do not appear helpful for predicting F1 performance.
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  • 14
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    Euphytica 101 (1998), S. 9-16 
    ISSN: 1573-5060
    Keywords: heading time ; Triticum aestivum ; vernalisation response ; Vrn – genotypes ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Substitution lines with reciprocal substitutions of chromosomes containing recessive alleles of the homoeologous group 5 chromosomeVrn genes between varieties of winter wheat with high vernalisation requirement (‘Mironovskaya 808’) and low vernalisation requirements (‘Bezostaya 1’) have been created. On this basis the genetic determination of vernalisation requirement was established. Substitution lines Mironovskaya 808 (Bezostaya 1 5A), Mironovskaya 808 (Bezostaya 1 5B), Mironovskaya 808 (Bezostaya 1 5D) and reciprocal substitution lines Bezostaya 1 (Mironovskaya 808 5A), Bezostaya 1 (Mironovskaya 808 5B) and Bezostaya 1 (Mironovskaya 808 5D) were grown under different durations of vernalisation (3, 4, 5, 6, 7 and 8 weeks) and their response was evaluated. Photoperiodic sensitivity of the original parental genotypes was also determined. Reciprocal substitution lines of the same chromosome that carries the same vrn allele responded differently to vernalisation deficit. Differences have been shown between all group 5 reciprocal substitutions. Lines carrying chromosomes 5A and 5D of Mironovskaya 808 had a high vernalisation requirement whereas lines carrying chromosome 5B of Bezostaya 1 (vrn2B) had a low vernalisation requirement. The reciprocal lines had a reverse requirement. This explains the different vernalisation requirements of the original varieties: Mironovskaya 808 with a high vernalisation requirement carries two alleles (vrn1M and vrn3M) in its genotype that increase the vernalisation requirement, whereas Bezostaya 1 with a lower requirement for vernalisation contains only one such allele (vrn2B). By combination of the alleles in the lines with the substitution of chromosome 5B carrying vrn2 allele that in both original genotypes work inversely to the other alleles, transgressive genotypes have been formed: genotype vrn1M vrn2B vrn3M determines a higher vernalisation requirement than original variety Mironovskaya 808, and genotype vrn1B vrn2M vrn3B determines a lower vernalisation requirement than the original Bezostaya 1. An incomplete vernalisation requirement prolonged the time to heading, with exponential dependence on the vernalisation deficit, or prevented heading altogether. The original varieties further differed in photoperiodic sensitivity (Mironovskaya 808 sensitive, Bezostaya 1 less sensitive) that also influenced the background of substitution lines. The impact of the background on the heading time showed itself by about one week difference between Mironovskaya 808 and Bezostaya 1 grown under 8 weeks vernalisation and normal photoperiod. The difference between the lines with Mironovskaya 808 background and the lines with Bezostaya 1 background was approximately the same and was not significantly changed in different vernalisation variants of the lines. This difference may be caused by different photoperiodic sensitivity of the original varieties, but also by other genes, such as genes of earliness per se.
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  • 15
    ISSN: 1573-5060
    Keywords: bread wheat ; Triticum aestivum ; tolerance to deoxynivalenol ; somaclonal variant ; in vitro selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This study was conducted to develop an efficient in vitro selection system for scab resistance by using in vitro screening for tolerance to deoxynivalenol (DON). Immature embryos of two wheat varieties, a scab-resistant variety Sumai 3 and a susceptible variety Mianyang 11, and their reciprocal F1 hybrids were cultured on MS medium supplemented with 2,4-D 2 mg/l and 0.6 × 10-4 M DON for callus induction. The responses of callus induction and plant regeneration to 0.6 × 10-4 M DON differed significantly between resistant and susceptible varieties, according to observed scab resistance levels at the plant level in the field. The percentage of callus formation of resistant variety Sumai 3 on induction medium containing DON was higher than that of susceptible variety Mianyang 11. Regeneration of DON-tolerant calli on DON-containing differentiation medium differed significantly between Sumai 3 and Mianyang 11. Averaged across the DON-tolerant calli of two varieties and their reciprocals, regeneration of DON-tolerant calli was decreased 3-fold on DON-containing medium. By an inoculation test with conidiospores of Fusarium graminearum Schw, we obtained several resistant lines from progenies of regenerated plants from DON-tolerant calli. These somaclonal lines had lower disease scoring (reaction index, infected spikelets and disease incidence), shorter plants and better yield components than Sumai 3, a famous Chinese resistant variety.
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  • 16
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; Secale cereale ; T1BL.1RS ; chromosome substitution and translocation ; yield components
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The T1BL.1RS wheat (Triticum aestivum L.) - rye (Secale cereale L.) translocations have been of particular interest and are widely used in bread wheat breeding programs. The objective of this study was to determine the effect of the T1BL.1RS chromosome on grain yield and its components using 20 near-isolines of spring bread wheat cultivar ‘Seri M82’ (10 homozygous for chromosome 1B substitution and 10 homozygous for T1BL.1RS). The test lines have been produced by substituting the 1B chromosome in Seri M82 (T1BL.1RS, T1BL.1RS) through backrossing. Two field experiments were evaluated under optimum (five irrigations) and reduced (one irrigation) moisture conditions for two consecutive production cycles at the Mexican National Agricultural Research Institute, Ciudad Obregon, Sonora, Mexico. The presence of T1BL.1RS had a significant effect on grain yield, harvest index, grains/m2, grains/spike, 1000-grain weight, test weight, flowering date and physiological maturity in both moisture conditions. The agronomic advantage of the 1B substitution lines on above-ground biomass yield at maturity, spikes/m2and grain-filling duration was expressed only under the optimum moisture condition. The presence of T1BL.1RS increased grain yield 1.6% and 11.3% for optimum and reduced moisture conditions, respectively. These results encourage further use of T1BL.1RS wheats in improving agronomic traits, especially for reduced irrigation or rainfed environments.
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  • 17
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    Euphytica 104 (1998), S. 39-47 
    ISSN: 1573-5060
    Keywords: drought resistance ; diallel graph ; gene action ; excised-leaf water loss ; relative water content ; bread wheat ; osmotic adjustment ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Little information is available on the genetics of excised leaf water loss and relative water content in wheat. An experiment conducted on the F1 generation from a half-diallel set of crosses involving two drought tolerant, two moderately tolerant and two sensitive varieties was initiated to investigate the inheritance of excised-leaf water loss and relative water content. This experiment was conducted under glass-house and field conditions at tillering and anthesis stages of plant development. Additive gene action, in general, played a major role in determining the inheritance of these traits. General combining ability (GCA) was the main source of genetic variation among crosses, while specific combining ability (SCA) was negligible. Strong phenotypic correlations existed between per se performance and GCA effects in the majority of cases. Heterosis was unimportant. Genotype-environmental interactions and/or differential gene expression appeared to account for different results found between environments and growth stages, respectively. Selection for relative water content appeared to be more effective at anthesis, while for excised-leaf water loss at both stages of plant growth. In addition to drought resistance, wide differences for morphological characters and relative positions of parental arrays revealed the possibility of obtaining desirable segregants for drought stress conditions from the cross Kharchia 65 × WH 147.
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  • 18
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Puccinia recondita tritici ; leaf rust ; rust resistance ; partial resistance ; slow rusting ; durable resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Fifty-five spring bread wheat (Triticum aestivum L.) cultivars, mostly released between 1975 and 1991 in eight leaf rust-prone spring wheat growing regions of the former USSR, were tested in the seedling growth stage for reaction to 15 Mexican pathotypes of Puccinia recondita f. sp. tritici. In total, seven known and at least two unknown genes were identified, either singly or in combinations: Lr3 (7 cultivars), Lr10 (14), Lr13 (5), Lr14a (1), Lr16 (1), Lr23 (3); the unknown genes were identified in 14 cultivars. The first unknown gene could be either Lr9, Lr19, or Lr25; however, the second unknown gene in 9 cultivars was different from any named gene. Twelve of the 15 pathotypes are virulent for this gene, hence its use in breeding for resistance will be limited. The cultivars were also evaluated at two field locations in Mexico with two pathotypes in separate experiments. The area under the disease progress curve and the final disease rating of the cultivars indicated genetic diversity for genes conferring adult plant resistance. based on the symptoms of the leaf tip necrosis in adult plants, resistance gene Lr34 could be present in at least 20 cultivars. More than half of the cultivars carry high to moderate levels of adult plant resistance and were distributed in each region.
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  • 19
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    Euphytica 81 (1995), S. 299-303 
    ISSN: 1573-5060
    Keywords: allelism ; aphid resistance ; Diuraphis noxia ; inheritance ; wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Studies were conducted to determine the inheritance and allelic relationships of genes controlling resistance to the Russian wheat aphid (RWA), Diuraphis noxia (Mordvilko), in seven wheat germplasm lines previously identified as resistant to RWA. The seven resistant lines were crossed to a susceptible wheat cultivar Carson, and three resistant wheats, CORWA1, PI294994 and PI243781, lines carrying the resistance genes Dn4, Dn5 and Dn6, respectively. Seedlings of the parents, F1 and F2 were screened for RWA resistance in the greenhouse by artificial infestation. Seedling reactions were evaluated 21 to 28 days after the infestation using a 1 to 9 scale. All the F1 hybrids had equal or near equal levels of resistance to the resistant parent indicating dominant gene control. Only two distinctive classes were present and no intermediate types were observed in the F2 segregation suggesting major gene actions. The resistance in PI225262 was controlled by two dominant genes. Resistance in all other lines was controlled by a single dominant gene. KS92WGRC24 appeared to have the same resistance gene as PI243781 and STARS-9302W-sib had a common allele with PI294994. The other lines had genes different from the three known genes.
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  • 20
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; alien translocation lines ; monosomic analysis ; C-banding ; genomic in situ hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A leaf rust resistant wheat-rye translocation stock, ST-1, introduced from Japan, comprised distinct morphological types. One type possessed a T1BL·1RS chromosome with genes Lr26, Yr9 and Sr31. A second type carried a new gene, Lr45, located in a large segment of rye chromosome translocated to wheat chromosome 2A. Its structure was identified as T2AS-2RS·2RL. Despite the homoeology of the 2A and 2R chromosomes and the high level of compensation provided by the translocation, Lr45 was not normally inherited and is probably associated with agronomic deficiencies that will prevent its exploitation in agriculture.
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  • 21
    ISSN: 1573-5060
    Keywords: somaclonal variation ; somatic embryogenesis ; tissue culture ; wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Somatic embryogenesis was initiated from ‘immature embryos’ on Murashige-Skoog (MS) medium plus 2 mg.l-1 2,4-dichlorophenoxyacetic acid, 2% sucrose and 0.6% agarose. Somatic embryos were isolated and regenerated into whole green plants on MS medium devoid of 2,4-D. These regenerants were previously demonstrated to differ in their mitochondrial DNA organization. In order to estimate their characteristics three progenies of short-term culture regenerants and three progenies of long-term culture regenerants were analyzed and compared to the parental line. These somaclones obtained from the wheat variety Chinese Spring were evaluated for variation of 13 agronomic and morphological quantitative characters in comparison to the parental line. Significant variation was observed for plant height, spike length, main tiller diameter, between the somaclones regenerated from long-term culture and their parent. Differences were observed to increase with the duration of culture, leading to a significant modification of the structure of the plants. Several changes occurred during the somatic tissue cultures, but to a lesser extent than has previously been described in the literature.
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  • 22
    ISSN: 1573-5060
    Keywords: wheat/rye translocation ; in situ hybridization ; triticale x wheat hybrids ; Triticum aestivum ; Secale cereale
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Heptaploid hybrids between octoploid triticale and wheat were backcrossed as female parents with wheat to examine the rye chromosome distribution in the resultant progenies using genomic in situ hybridization (GISH). One hundred and one backcross (BC) seeds were examined and whole rye chromosome additions and substitutions, wheat/rye centric and noncentric translocations and rye telocentric chromosomes were detected. Dicentric wheat/rye translocated chromosomes were also observed. Comparisons were made with previous results on the rye chromosome distribution from male gametes of the same cross and differences were found, where in the female derived population a deficit of plants with more than two rye chromosomes was apparent relative to the anther derived population.
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  • 23
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    Euphytica 83 (1995), S. 193-197 
    ISSN: 1573-5060
    Keywords: biscuit-making quality ; Glu-B1 HMW-GS ; soft wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The aim of this study was to assess the effect of specificGlu-B1 HMW-GS on biscuit-making quality. Three soft spring wheat cultivars with the sameGlu-A1 andGlu-D1 HMW-GS, but differentGlu-B1 HMW-GS were used in crosses. F2∶4 derived lines were developed from these crosses.Glu-B1 HMW-GS 6+8 and 17+18; and 7+9 and 17+18 were compared. Lines with HMW-GS 6+8 versus those with HMW-GS 17+18 had a higher flour protein- and alveograph P/L ratio, shorter mixograph mixing time, more vitreous kernels, and a lower alveograph distensibility and strength (all values significant at p=0.05). Lines with HMW-GS 7+9 compared to those with 17+18 showed significant differences for flour extraction and biscuit diameter. The presence of HMW-GS 17+18 was significantly correlated with several biscuit-making quality characteristics in the Dirkwin/Zaragosa F2∶4 lines but not in the Waverley/Zaragosa F2∶4 lines, therefore the effect of HMW-GS 17+18 was modified by the genetic background in which they were expressed.
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  • 24
    ISSN: 1573-5060
    Keywords: friabilin ; grain quality ; wheat ; wheat grain hardness ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The end-use quality of wheat (Triticum aestivum L.) is determined in large part by the texture of the grain (soft or hard). Endosperm texture is currently determined by several empirical methods. These methods are limited because the use bulk grain lots, as opposed to individual kernels; assess phenotypic, as opposed to genotypic hardness; require a quantity of grain greater than that generally available in the early generations of wheat breeding programs, and are destructive. Recent approaches that use single kernels address the problems associated with bulk grain lots, but suffer the other limitations of providing only the phenotype and being destructive. An objective method for determining the texture genotype of single kernels of wheat was developed using starch granule-associated friabilin, a family of closely related 15 kDa proteins, as a biochemical marker. The occurrence of friabilin on water-washed wheat starch granules is apparently unaffected by the environment and is perfectly correlated (no exceptions) with grain softness. The technique presented here can detect friabilin on as little as 0.2 mg of starch and provides a 250-fold improvement in friabilin detection compared to previous methods. The method is capable of correctly assessing the genotype of F1 heterozygotes from hard x soft and soft x hard crosses. Further, the method uses only a portion of the endosperm from the kernel and therefore accommodates embryo propagation and high molecular weight glutenin subunit characterization. This single kernel method also facilitates the genetic characterization of mixed, bulk grain lots.
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  • 25
    ISSN: 1573-5060
    Keywords: Aegilops markgrafii ; aneuploids ; chromosome specific library ; DOP-PCR ; microdissection ; molecular markers ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary We established a chromosome specific DNA library of the Aegilops markgrafii chromosome B. Eight microdissected chromosomes B obtained from a monosomic T. aestivum-Aegilops markgrafii addition line were PCR-amplified and the DNA was cloned in Escherichia coli DH5α. Clones were characterized by dot blot hybridization with total Ae. markgrafii DNA. 62% of clones represented repetitive sequences and 38% low or single copy sequences. The estimated length of excised inserts varied between less than 200 bp and more than 500 bp. The average size of inserts was 310 bp.
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  • 26
    ISSN: 1573-5060
    Keywords: Bread-making quality ; chromosome effects ; grain hardness ; SDS-sedimentation volume ; substitution lines ; wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The group 1 and 6 inter-varietal chromosome substitution lines of Cappelle-Desprez (Bezostaya 1) were intercrossed along with the donor and recipient varieties, Cappelle-Desprez and Bezostaya 1, to give 36 genetically different families. The analysis of the means of these families showed that variation in SDS-sedimentation volume fitted a predominantly additive model. There were no significant within or between chromosome interactions among the group 1 and 6 chromosomes. Nor was there any evidence for interactions between these chromosomes and those of the background. Significant dominance/within chromosome interactions amongst the background chromosomes were however detected. Some of the positive effects on SDS-sedimentation were associated with increased grain hardness. Chromosome effects on % grain protein were not correlated with SDS-sedimentation.
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  • 27
    ISSN: 1573-5060
    Keywords: agronomic characters ; QTL ; RFLP maps ; wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The advent of molecular marker systems has made it possible to develop comparative genetic maps of the genomes of related species in the Triticeae. These maps are being applied to locate and evaluate allelic and homoeoallelic variation for major genes and quantitative trait loci within wheat, and to establish the pleiotropic effects of genes. Additionally, the known locations of genes in related species can direct searches for homoeologous variation in wheat and thus facilitate the identification of new genes. Examples of such analyses include the validation of the effects of Vrn1 on chromosome 5A on flowering time in different crosses within wheat; the indication of pleiotropic effects for stress responses by the Fr1 locus on chromosome 5A; the detection of homoeologous variation for protein content on the homoeologous Group 5 chromosomes; and the detection of a new photoperiod response gene Ppd-H1 in barley from homoeology with Ppd2 of wheat.
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  • 28
    ISSN: 1573-5060
    Keywords: aneuploids ; automated DNA sequencer ; chromosomal location ; microsatellite marker ; PCR ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The chromosomal assignment of 64 PCR-amplified microsatellite loci and 29 additional fragments amplified by the same primer pairs is described for bread wheat (Triticum aestivum). The distribution over the different chromosomes and chromosome arms appears to be random. The highest proportion of microsatellite loci is found on the B genome, followed by the A and D genome. About half of the primer pairs amplified unique fragments, while the other half amplified additional fragments. 25% of the primer pairs, mostly designed to clones of a PstI-library, amplify fragments on homoeologous chromosomes. In some cases, more than one fragment on a single chromosome or fragments on non-homoeologous chromosomes occurred. The use of an automated DNA sequencer accounts for the accurate resolution of multiple fragments and enables to differentiate between fragments, amplified by a single primer pair, with size differences as small as two base pairs.
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  • 29
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    Euphytica 89 (1996), S. 59-64 
    ISSN: 1573-5060
    Keywords: earliness ; photoperiodic response ; Triticum aestivum ; bread wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The photoperiodic response of 15 spring and winter varieties was observed. Vernalised plants were grown under long-day (light period longer than 14-hours) and under short-day conditions (light period 10-hours). The earliness under long-day condition and the photoperiodic responses were significantly different amongst the tested varieties. The earliness under long-day conditions depended on photoperiodic response. The winter-spring vernalisation requirement influenced earliness but did not influence photoperiodic response. Varieties with spring growth habits that had the same or similar photoperiodic response to varieties with winter growth habit were earlier in heading. The tested varieties that came from lower geographical latitudes were usually earlier and less photoperiod sensitive than the varieties from higher geographical latitudes.
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  • 30
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    Euphytica 89 (1996), S. 49-57 
    ISSN: 1573-5060
    Keywords: adaptability ; earliness per se ; photoperiod sensitivity ; vernalization sensitivity ; wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In order to obtain high levels of environmental adaptability in wheat varieties it is essential they flower at times appropriate to particular environmental conditions. The influence of three distinct genetic systems that together determine time of flowering is reviewed here. Vernalization genes are seen to be particularly important to winter wheats for their direct or indirect effects on winter hardiness. Vernalization genes play a minor role in determining flowering time in autumn sown winter wheats but insensitivity is essential if spring sown wheats are to flower. Day length sensitive photoperiod genes play a major role in determining flowering time and adaptability of autumn sown wheats. Insensitivity can promote yield advantages of over 35% in Southern European environments. 15% in Central Europe and offers benefits even in the UK. At present only a single allele of Ppd1 appears to have been introduced into commercial European wheat varieties. The merits of alternative Ppd1 alleles or different loci are discussed. The influence of earliness per se genes that determine flowering time independently of environmental stimuli is less well documented than the effect of photoperiod and vernalization genes. It is likely that genes on chromosomes belonging to groups 2, 3, 4, 6 and 7 may act to modify flowering time independently of environmental stimuli probably by determining numbers of vegetative and floral primordia being initiated or the rate of initiation of the primordia. Earliness per se genes appear to be widespread in European wheats and play a significant role in determining the exact time plants flower.
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  • 31
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    Euphytica 89 (1996), S. 65-68 
    ISSN: 1573-5060
    Keywords: bread wheat ; Triticum aestivum ; Secale cereale ; Triticum sphaerococcum ; Vrn genes ; introgression ; genetic analysis ; Vrn6 Sc ; Vrn7 Sc ; Vrn8 Tc identification ; rye
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Alien dominant genes of spring habit were introgressed into bread wheat. The introgression was undertaken by simple crossing of winter bread wheat to related spring species or genera, followed by backcrossing to winter bread wheat, and did not involve the use of the ph mutants or embryo culture. The introgressed genes were located mostly on chromosomes of homoeologous group 5, and were allelic to the known Vrn genes in bread wheat. Nevertheless three groups of lines were discovered with the genes possibly located on other chromosomes. These genes were non-allelic to each other and to known Vrn genes and were designated Vrn6 Sc , Vrn7 Sc (introgressed from Secale cereale) and Vrn8 Ts (from Triticum sphaerococcum).
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  • 32
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    Euphytica 89 (1996), S. 69-75 
    ISSN: 1573-5060
    Keywords: dwarfing genes ; gene mapping ; GA insensitivity ; rye ; Secale cereale ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The improvement of lodging resistance by introducing major dwarfing genes, classified either as GA insensitive or GA sensitive, is one of the main strategies chosen by cereal breeders. In the present paper the current knowledge about the genetics, chromosomal localisation and the homoeoallelic relationships of the dwarfing genes in wheat and rye is reviewed. The confusing system of the symbolisation of the GA insensitive dwarfing genes/alleles in wheat is discussed and a nomenclature based on rules for gene symbolisation in wheat is proposed.
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  • 33
    ISSN: 1573-5060
    Keywords: Chromosomal location ; grain size ; wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Grain size in wheat is the most stable yield component and has a favorable effect on flour yield. To identify the chromosomes associated with the large grains of line G603-86, (grain weight over 60 mg and grain length of about 9 mm), F3 lines, extracted from F2 populations obtained from F1 monosomics of crosses between G603-86 (P1) and the monosomic set of Favorit (P2) were tested in the field. ANOVA showed significant differences among parents for grain weight and grain length, but not for grain width or the factor expressing the difference in grain form and density. Homoeologous groups had significant effects on grain weight and on all components of grain weight, while genomes were not significantly different for any of these characters. Grain weight was significantly increased by chromosomes 6D and 4A of G603-86. Grain length was significantly increased by chromosomes 4A, 4B, 2B, 3A and 1B, grain width by chromosomes 1A and 1B, and the factor form-density by chromosomes 6D and 6A. The high grain size in G603-86 results from the effects of genes located on many chromosomes which affect grain dimensions, form and density.
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  • 34
    ISSN: 1573-5060
    Keywords: chromosome substitutions ; hybrid dwarfness ; photoperiodic response ; tissue culture response ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The wheat varieties Chinese Spring, Marquis and Thatcher and five intervarietal ‘Chinese Spring’ substitution lines for chromosomes 2B (2 lines), 2D (2 lines) and 4A*, differing from the recipient variety in alleles for hybrid dwarfing genes and/or the photoperiodic response genes were analysed for tissue culture response (TCR). It could be demonstrated that only chromosome 2B has a major effect on TCR. Allelic variation at the hybrid dwarfing loci seems to have no effect on tissue culture performance, even in the combination D1D2D3 which gives the grass dwarf phenotype. Also the allelic constitution at the Ppd loci, gave no indication for a direct major effect of those alleles, however there seems to exist genetical factors for TCR on the homoeologous group 2 chromosomes which may be closely linked to the Ppd loci.
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  • 35
    ISSN: 1573-5060
    Keywords: Aneuploidy ; dwarfing genes ; Triticum aestivum ; preferential transmission ; Aegilops sharonensis ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Wheat varieties tend to be chromosomally unstable producing on average 2–3% of plants with abnormal chromosome numbers. A number of semi dwarf wheat varieties, carrying the gibberellic acid insensitive dwarfing genes Rht1 or Rht2, have been seen to produce distinct tall off types due to reduction in dosage of the chromosome carrying the dwarfing gene. The UK variety ‘Brigand’, carrying Rht2 on chromosome 4D, produced very distinct tall off types when this chromosome was reduced in dosage. The frequency of tall off types was sufficiently high to cause the variety to fail United Kingdom statutory uniformity tests. An attempt to prevent the loss of chromosome 4D was made by constructing translocation chromosomes involving the short arm of chromosome 4D, which carries Rht2, and the long arm of chromosome 4S l from Aegilops sharonensis, which carries a gene(s) conferring preferential transmission. The work in this paper describes the field evaluation of two lines carrying 4DS.4DL-4S l L translocations, and demonstrates their success in preventing spontaneously occurring monosomy of chromosome 4D in semi-dwarf wheats.
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  • 36
    ISSN: 1573-5060
    Keywords: C-banding ; FISH ; heterochromatin ; in situ hybridization ; rye ; Secale cereale ; Secale montanum ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The sequential combination of C-banding and in situ hybridization techniques applied in this or in a reverse order, are used to recognize targeted chromosomal regions in cereals. Both methods are described whereby standard chromosome squash preparations are followed by: i) C-banding technique using Leishman stain and a slightly modified in situ hybridization technique using biotin-labeled DNA probes, or ii) fluorescence in situ hybridization technique and C-banding. Both approaches have been successfully used onto mitotic chromosomes of rye and wheat resulting suitable for both their identification and detection of targeted sites.
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  • 37
    ISSN: 1573-5060
    Keywords: alien introduction ; chromosome pairing ; fluorescent in situ hybridization ; wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Fluorescent in situ hybridization (FISH) of DNA to plant chromosomes has proved to be a powerful cytogenetic tool. The value of fluorescent in situ hybridization of total genomic DNA (GISH) of related species is demonstrated in the determination of wheat/alien chromosome pairing in hybrids. Its use for assessing the relative merits of the various genes that affect chromosome pairing is also shown. The ability of GISH to identify the presence in wheat of whole alien chromosomes or alien chromosome segments is illustrated. The potential of FISH for detecting repeated DNA sequences, low copy sequences and single copy genes is discussed.
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  • 38
    ISSN: 1573-5060
    Keywords: Aegilops spp. ; alien introgression ; aneuploidy ; cytogenetics ; history ; Plant Breeding Institute of the University Halle ; Secale cereale ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The historical development of cytogenetic research in cereals performed at the Plant Breeding Institute of the Martin-Luther-University Halle-Wittenberg from its beginning in 1935 until 1992 is reviewed with special reference to polyploidy, alien introgression and aneuploidy. Th. Roemer founded 1935 in the framework of his Institute a Department of Mutation Research which, in 1937, was extended to a Department of Cytogenetics with R. Freisleben as the first head. Research highlights of this period were the introduction of mutation breeding, the development of autotetraploids in barley and linseed, the discovery of the crossability genes in wheat and the performance of wheat-rye crosses. The main objective in the period between 1950–1960 was the analysis of the relationships between chromosome behaviour and seed set in tetraploid rye and octoploid triticale. Since 1961 the Cytogenetics Research Group was headed by D. Mettin; he was followed by W.D. Blüthner in 1983. The research activities in this period concerning aneuploidy in rye and wheat and alien introgression are being reviewed under the following headings: Cytogenetics of rye; work with wheat aneuploids; contributions to the IR introgression into wheat; alien introgressions into wheat to improve disease resistance and grain quality; the exploitation of molecular markers.
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  • 39
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    Euphytica 89 (1996), S. 215-221 
    ISSN: 1573-5060
    Keywords: agronomic characteristics ; breeding trends ; quality ; year of release ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Wheat breeding efforts for a half century in Japan were investigated by using of 129 varieties registered in MAFF from 1929 to 1984 in the view point of case history for three years. Varieties released in each breeding station are classified apparently by growth habit associated closely with duration of the cold requirement. Heading date is earlier in varieties released in southwest breeding stations (southern varieties) than in varieties released in northern breeding stations (northern varieties). Culm length is higher and the pre-harvest sprouting is more sensitive in northern varieties. In quality characters, northern varieties has the higher milling rate, lower apparent amylose content and larger particle size of flour. In varieties released in the Tohoku district, trend of breeding direction with time is smaller ear numbers and larger 1,000-grain weight and greater resistance to powdery mildew. In varieties released in the Kyushu district, a significant correlation with released year was clearly observed with regard to early maturity, short culm length, less grain crude protein content, less apparent amylose content and higher milling rate on breeding advancement.
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  • 40
    ISSN: 1573-5060
    Keywords: hybrid wheat ; Triticum aestivum ; plant height ; internode length ; heterosis ; combining ability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The heterosis and combining ability for plant height and its components of hybrid wheat were investigated in an incomplete diallel experiment including 5 CMS lines and 4 restorer lines. The results showed that heterosis (HS) and heterobeltiosis (HBS) occurred in plant height (PH) and length of the first internode (LFI), second internode (LSI), third internode (LTI), basal internode (LBI) and the spike (LS) of hybrids, but their values varied among crosses and characters; the HS and HBS of LBI were larger than those of other characters, the HS and HBS of LSI and LTI contributed a lot to those of PH. There were significant relationships between internode lengths and PH for specific combining ability (SCA) and general combining ability (GCA), and among lengths of the adjacent internodes for SCA and/or GCA effects. However, the relationships of LS with the lengths of internodes and PH were insignificant for GCA, SCA, HS and HBS. The SCA effects were more important than GCA effects for LFI, the reverse was true for LSI, LTI, LS and PH, and the SCA effects was nearly equal to the GCA effects for LBI. So, LFI was mainly influenced by non-additive effect of genes, while LSI, LTI, LS and PH were mainly controlled by additive gene effects, LBI was controlled equally by additive and non-additive effects of genes. The genes that control the length of specific internode not only affect PH, but also the length of the adjacent internode. The genetic system in charge of lengths of internodes and plant height is independent of that for length of spike. Thus, it is possible to develop new wheat cultivars or hybrid combinations having long spike but dwarf plant height.
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  • 41
    ISSN: 1573-5060
    Keywords: Puccinia striiformis ; Triticum aestivum ; association of components ; stripe rust ; durable resistance ; infection frequency ; latency period ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Latency period, infection frequency, lesion length, lesion growth, disease severity and percentage of infected leaf parts were assessed on 10-day-old seedling leaves and flag leaves of ten bread wheat (Triticum aestivum L.) cultivars after inoculation with urediospores of Puccinia striiformis Westend. f. sp. tritici. For all components significant genotypic differences were detected. Components of resistance tended to be associated. A long latency period was associated with a low infection frequency, small lesions, a low disease severity and a low percentage of infected leaf parts. The latency period, measured as time period until first pustule appearance (LP1), was highly correlated with the latency period measured as time period until 50% of the pustules appeared (LP50). Assessment of latency period of large numbers of cultivars could therefore be reliably done by measuring LP1 which is less time consuming than measuring LP50. Latency period, infection frequency and disease severity were highly correlated with disease development data from field experiments. These results suggest that selection in the greenhouse for one of these components should result in cultivars with high levels of quantitative resistance. Disease severity after uniform inoculation in the greenhouse can be used for monocyclic evaluations because it is the easiest to assess.
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  • 42
    ISSN: 1573-5060
    Keywords: heading character ; Hordeum vulgare ; Triticum aestivum ; wheat-barley chromosome addition line
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Heading time in cereals is a composite character determined by vernalization requirement, photoperiodic sensitivity and narrow-sense earliness. To study the effects of added barley chromosomes on the heading characters in wheat, two sets of wheat-barley chromosome addition lines, i.e., ‘Betzes’ barley chromosomes 2H to 7H added to ’Chinese Spring‘ wheat (CS-Be2H to CS-Be7H) and ‘New Golden’ barley chromosomes 5H and 6H added to ‘Shinchunaga’ wheat (Shi-NG5H, Shi-NG6H), were examined for their heading characters. All barley chromosomes except Be6H affected vernalization requirement and/or narrow-sense earliness in CS or Shi. Be5H chromosome also slightly increased the photoperiodic sensitivity of CS. Shi-NG5H addition line showed significantly decreased vernalization requirement in comparison with Shi, whereas CS-Be5H did not show any difference from CS. The F1 hybrid of the cross, Shi-NG5H × CS-Be5H, exhibited the same level of vernalization insensitivity as the Shi-NG5H addition line, and plants with and without a vernalization requirement segregated in a 1 : 3 ratio in the F2 generation. These observations, together with previous reports, suggest that the decreased vernalization requirement in the Shi-NG5H addition line was caused by the presence of a major dominant gene for spring habit, Sh2, located on the NG5H barley chromosome. Furthermore, this study revealed that the Sh2 gene in barley has a similar but weaker effect than the wheat vernalization insensitive gene, Vrn1, on the vernalization response in wheat.
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  • 43
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Puccinia striiformis ; stripe rust ; epidemiology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Ten spring bread wheat cultivars with a susceptible seedling reaction to race 14E14 of yellow rust were tested at three locations to assess the level and stability of quantitative resistance. Quantitative resistance was expressed in terms of disease severity (DS), area under the disease progress curve (AUDPC), apparent infection rate (r), infection type (IT) and infection density. Large genotypic differences were observed for all variables measured. Morocco was the most susceptible cultivar. Based on its high infection type (IT=9) throughout the epidemics, it most likely does not possess any resistance. All other cultivars carry quantitative resistance. The levels ranged from very low (Taichung 23) to very high (Parula). Resistance levels were lower in Quito, Ecuador than at the other locations. Most likely, the lower temperatures in Quito resulted in a reduced expression of quantitative resistance to yellow rust and to obtain the same protection as at the other two locations, more resistance genes are needed. Therefore, to accumulate genes for quantitative resistance, Quito is considered to be the better location. Though significant cultivar-location interactions were detected, they were small compared to the cultivar and location effect. Therefore, they are considered of little importance and it is concluded that quantitative resistance is a stable trait, in the sense that cultivar rankings are hardly affected by environment. The contribution of infection growth to the development of yellow rust was demonstrated. Between 29 and 66% of the increase in disease severity could be contributed to growth of infections. These figures are probably an underestimation of the real contribution as new infections are very small, thus reducing the average size of infections and their contribution to the increase of disease severity.
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  • 44
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    Euphytica 91 (1996), S. 31-35 
    ISSN: 1573-5060
    Keywords: aluminum ; linkage map ; marker ; RFLP mapping ; Triticum aestivum ; Triticum turgidum ; wheat ; durum wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The tolerance of aluminum (Al) of disomic substitution lines having the chromosomes of the D genome of Triticum aestivum L. cv. Chinese Spring individually substituted for their homoeologues in T. turgidum L. cv. Langdon was investigated by the hematoxylin method. The disomic substitution lines involving chromosome 4D were more Al tolerant than Langdon. The tolerance was found to be controlled by a single dominant gene, designated Alt2, that is in the proximal region of the long arm of chromosome 4D. The locus was mapped relative to molecular markers utilizing a population of recombinant chromosomes from homoeologous recombination between Chinese Spring chromosome 4D and T. turgidum chromosome 4B. Comparison of the location of Alt2 in this map with a consensus map of chromosomes 4B and 4D based on homologous recombination indicated that Alt2 is in a vicinity of a 4 cM interval delineated by markers Xpsr914 and Xpsr1051. The Alt2 locus is distal to marker Xpsr39 and proximal to XksuC2. The Altw locus is also proximal to the Knal locus on chromosome 4D that controls K+/Na+ selectivity and salt tolerance. In two lines, Alt 2 and Knal were transferred on a single 4D segment into the long arm of T. turgidum chromosome 4B.
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  • 45
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat-alien translocation ; resistance ; C-banding ; in situ hybridization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Wild relatives of common wheat, Triticum aestivum, and related species are an important source of disease and pest resistance and several useful traits have been transferred from these species to wheat. C-banding and in situ hybridization analyses are powerful cytological techniques allowing the detection of alien chromatin in wheat. C-banding permits identification of the wheat and alien chromosomes involved in wheat-alien translocations, whereas genomic in situ hybridization analysis allows determination of their size and breakpoint positions. The present review summarizes the available data on wheat-alien transfers conferring resistance to diseases and pests. Ten of the 57 spontaneous and induced wheat-alien translocations were identified as whole arm translocations with the breakpoints within the centromeric regions. The majority of transfers (45) were identified as terminal translocations with distal alien segments translocated to wheat chromosome arms. Only two intercalary wheat-alien transloctions were identified, one induced by radiation treatment with a small segment of rye chromosome 6RL (H25) inserted into the long arm of wheat chromosome 4A, and the other probably induced by homoeologous recombination with a segment derived from the long arm of a group 7 Agropyron elongatum chromosome with Lr19 inserted into the long arm of 7D. The presented information should be useful for further directed chromosome engineering aimed at producing superior germplasm.
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  • 46
    ISSN: 1573-5060
    Keywords: common wheat ; Triticum aestivum ; powdery mildew ; resistance gene ; monosomic analysis ; gene location
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Common wheat cultivar Virest possesses mildew resistance which is different from resistances expressed by currently documented mildew resistance genes, detected by response to eleven differential wheat powdery mildew isolates. F2 populations from hybrids of the 21 ‘Chinese Spring’ monosomic lines with ‘Virest’ revealed one major dominant gene, located on wheat chromosome 1D. The new gene is designated Pm22. Italian cultivars Elia, Est Mottin, Ovest and Tudest also showed the disease response pattern corresponding to ‘Virest’.
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  • 47
    ISSN: 1573-5060
    Keywords: adaptation ; intrinsic earliness ; Triticum aestivum ; vernalization ; wheat ; Syria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary This study sought to identify factors that influence wheat development in the transitional wheat growing zone of northern Syria. Three development factors were studied, intrinsic earliness, and responses to vernalization and to photoperiod. Two sets of wheat were studied, each composed of lines with differing combinations of development factors. Set 1 comprised 20 parental and breeding lines utilized by the CIMMYT/ICARDA facultative and winter wheat breeding program based at Tel Hadya. Set 2 comprised 19 parental and breeding lines utilized by an Australian winter wheat breeding program based at Temora. Field development was recorded in greatest detail at one site. Tel Hadya, using the state of differentiation of the apex of the main tiller of sampled plants. To extend findings, development was also recorded as the time from sowing to ear emergence for later sowings of wheat at Tel Hadya, and in sowings at four other regional sites. The significance of each development factor was tested in multiple regressions that predicted either stage of apical development at Tel Hadya, or time to ear emergence in all trials. It was found that intrinsic earliness was the major factor associated with development, in both sets of wheat. Response to photoperiod had a much smaller and less consistent effect. Response to vernalization had least effect on development, possibly because low temperature in winter delayed development for a longer period than was required to fully vernalize winter wheats. Our results suggested it may not be directly relevant whether spring or winter wheats are grown in the transitional zone of northern Syria. The desired phenotype for the region, of slow development prior to double ridge, then fast development to ear emergence, cannot be simply achieved from combinations of the three development factors. Selection for improved adaptation to the region must continue to rely on direct field observations.
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  • 48
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    Euphytica 91 (1996), S. 297-303 
    ISSN: 1573-5060
    Keywords: bread wheat ; gibberellin sensitivity ; male sterility ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effect of gibberellic acid (GA3) on male fertility was investigated in wheat. Greenhouse and field experiments were carried out. GA3 induced high levels of male sterility both in gibberellin (GA)-sensitive and GA-insensitive genotypes. The optimum concentration was 2000 ppm of GA3 when applied at successive sprays. The critical period for GA3 treatment, in the general sense, extended from glume differentiation to premeiotic interphase in the oldest florets of the spike, though differences were found between GA-sensitive and GA-insensitive genotypes in the extension of this period. The effect of GA3 on plant height was also studied. The potential use of GA3 as a chemical hybridizing agent in wheat breeding is discussed.
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  • 49
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    Euphytica 92 (1996), S. 147-153 
    ISSN: 1573-5060
    Keywords: breeding methodology ; drought patterns ; input-efficiency ; wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary About 32% of the 99 million ha wheat grown in developing countries experiences varying levels of drought stress. Three major drought types have been identified: Late drought (LD) is common in the Mediterranean region, early drought (ED) is found in Latin America and wheat is produced on residual soil moisture (RM) in the Indian subcontinent and part of Australia. Until 1983, CIMMYT selected all germplasm under near optimum conditions for its yield potential and tested only advanced lines under drought. In spite of many critics, this approach proved to be successful, since in the mid 80's CIMMYT germplasm was grown on 45% of the wheat area in LC with annual rainfall from 300–500 mm and on 21% in areas with less than 300 mm. Since 1983, CIMMYT's drought breeding methodology is to alternate segregating populations between drought stressed and fully irrigated conditions (FI) and to test advanced lines under a line source irrigation system. To compare the efficiency of these approach, yield of four, mostly leading varieties, from each of the regions with LD, ED, RM, and FI and twelve recent CIMMYT cultivars selected for high yield under FI and RM conditions (ALT) were compared under four different moisture regimes (FI, LD, ED, and RM) in 89–90 and 90–91 in Yaqui Valley, Mexico. Genotypic correlation between yield and days to flowering, days to maturity, height, grains m-2, TKW, test weight and grain fill period were calculated. Mean grain yield of the four best lines in the ALT group was highest under all moisture stress regimes, followed by the FI-group. However, the highest yielding cultivar within each moisture regime was from the FI-group under FI, from the LD-group under LD, and from the ALT-group under ED and RM conditions. Estimates for genetic advance suggest that FI is the best environment for increasing grain yield even in all three drought environments. This indicates that yield potential per se is beneficial also in drought environments. The highest yield in drought environments was realized by the CIM cultivars selected under FI and RM. Simultaneous evaluation of the germplasm under near optimum conditions, to utilize high heritabilities and identify lines with high yield potential, and under stress conditions to preserve alleles for drought tolerance seem at present the best strategy.
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  • 50
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    Euphytica 92 (1996), S. 221-224 
    ISSN: 1573-5060
    Keywords: hybrid lines ; nitrogen efficiency ; variation ; wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The new European Common Agricultural Policy and environmental considerations are certainly to change agricultural practices toward low input cultivation systems. Nitrogen is one of the main inputs of winter wheat in northern France and it contributes highly to phreatic water pollution. A research programme has then been set up in order to study whether it is possible to breed for winter wheat cultivars using more efficiently N fertilisers. Less nitrogen would be applied, decreasing pollution risks and operational costs. It has been shown that a large variation exists for N related traits and for the resistance against N deficiency. On the one hand the cv Arche is very resistant to N deficiency, its yield on low N conditions (with no N fertiliser) is on average 89% of its yield on high N conditions (with a high N application). On the other hand, cv Récital is very susceptible to N deficiency as this same percentage is only 61%. A study on 10 hybrids showed that heterosis for grain yield was higher at low N level than at high N level. This was due to a higher number of grains per m2.
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  • 51
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    Euphytica 92 (1995), S. 301-306 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; bread wheat ; Secale cereale ; rye ; crossability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Crossability of bread wheat (Triticum aestivum L.) from Japan with rye (Secale cereale L.) was investigated by controlled pollination. No normal seeds were produced, but numbers of shrivelled and small seeds with embryos were used to estimate crossability amongst the 96 accessions, viz: 0–10% (29), 10–30% (23), 30–50% (11), 50–90% (33). The investigation for the pedigrees of varieties with more than 50% crossability percentages showed that the kr alleles of some accessions derived from common ancestors.
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  • 52
    ISSN: 1573-5036
    Keywords: boron ; rice-wheat rotation ; sterility ; time of sowing ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Spikelet sterility in wheat (Triticum aestivum L.) is emerging as a production threat in different parts of Nepal. This study was aimed at determining the effects of sowing date and boron application in controlling spikelet sterility in four different genotypes of spring wheat in a rice-wheat system in the western hills of Nepal. Four genotypes of known different responses to boron were planted on 21 November, 6 December and 21 December, 1994 with or without boron application at 1 kg B ha-1 (i.e. 9 kg borax ha-1) on a soil that was known to be deficient in boron. The effect of sowing date was significant for the phenology, yield components, percentage sterility and grain yield. Sterility was significantly increased in the crop planted on 21 December, which had also the lowest 1000 seed weight and grain yield; there was an almost 50% grain yield reduction compared to the crop planted on 21 November. Terminal moisture stress (i.e. lack of moisture during the later part of the development) was observed in the late sown crop which also amplified the extent of sterility associated with boron deficiency. Genotypes differed in response to sowing dates and boron treatment for all of the phenological events measured, yield components, grain yield and percentage sterility. SW-41 and BL-1022 had significantly higher sterility at all sowing dates. BL-1249 showed a consistently lower% sterility over all sowing dates and boron treatments. The addition of boron significantly increased the number of grains set per spike thereby decreasing the total sterility in boron responsive genotypes SW-41 and BL-1022 while those not susceptible did not respond. The boron concentration in the flag leaf at anthesis was increased in treatments with added B in the soil but genotypes did not differ in boron concentration for any soil treatment.
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  • 53
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    Plant and soil 215 (1999), S. 65-72 
    ISSN: 1573-5036
    Keywords: barley ; boron deficiency ; Hordeum vulgare ; Triticum aestivum ; variation ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Responses of a range of barley (Hordeum vulgare L.) genotypes to boron (B) deficiency were studied in two experiments carried out in sand culture and in the field at Chiang Mai, Thailand. In experiment 1, two barley genotypes, Stirling (two-row) and BRB 2 (six-row) and one wheat (Triticum aestivum L.) genotype, SW 41, were evaluated in sand culture with three levels of applied B (0, 0.1 and 1.0 μM B) to the nutrient solution. It was found that B deficiency depressed flag leaf B concentration at booting, grain number and grain yield of all genotypes. In barley Stirling, B deficiency also depressed number of spikes plant-1, spikelets spike-1 and straw yield. However, no significant difference between genotypes in flag leaf B concentration was found under low B treatments. Flag leaf B concentration below 4 mg kg-1 was associated with grain set reduction and could, therefore, be used as a general indicator for B status in barley. In experiment 2, nine barley and two wheat genotypes were evaluated in the field on a low B soil with three levels of B. Boron levels were varied by applying either 2 t of lime ha-1 (BL), no B (B0) or 10 kg Borax ha-1 (B+) to the soil prior to sowing. Genotypes differed in their B response for grain spike-1, grain spikelet-1 and grain set index (GSI). The GSI of the B efficient wheat, Fang 60, exceeded 90% in all B treatments. The B inefficient wheat SW 41 and most of the barley genotypes set grain normally (GSI 〉80%) only at the B+. In B0 GSI of the barley genotypes ranged from 23% to 84%, and in BL from 19% to 65%. Three of the barley with severely depressed GSI in B0 and BL also had a decreased number of spikelets spike-1. In experiment 3, 21 advanced barley lines from the Barley Thailand Yield Nursery 1997/98 (BTYN 1997/98) were screened for B response in sand culture with no added B. Grain Set Index of the Fang 60 and SW 41 checks were 98 and 65%, respectively, and GSI of barley lines ranged between 5 and 90%. One advanced line was identified as B efficient and two as moderately B efficient. The remaining lines ranked between moderately inefficient to inefficient. These experiments have established that there is a range of responses to B in barley genotypes. This variation in the B response was observed in vegetative as well as reproductive growth. Boron efficiency should be considered in breeding and selection of barley in low B soils.
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  • 54
    ISSN: 1573-5036
    Keywords: biomarker ; cadmium ; copper ; heavy metal ; PC ; PC-SH ; phytochelatin ; stress ; toxicity ; Triticum aestivum ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Heavy metal contaminated soils often show increased levels of more than one metal, e.g. copper (Cu), cadmium (Cd), zinc (Zn), lead (Pb) or nickel (Ni). In case such soils are used for crop production, prediction of yield reduction or quality decline due to heavy metals in the soil is inadequate when based only on chemical soil analysis. The use of biomarkers such as phytochelatins (PC), non-protein thiols specifically induced in plants upon exposure to heavy metals, may be an additional tool or diagnostic criterion in heavy metal research and in practice. In the present work, Cu and Cd uptake and induction of PC synthesis are studied with hydroponically grown maize and wheat plants exposed to mixtures of the two metals. We observed a close positive relationship between the concentrations of Cd and PC in the plant shoot material. A decreased shoot concentration of Cd after addition of Cu, due to metal competition at common root absorption sites, coincided with lower shoot PC levels. Also differences in metal uptake and xylary metal transport among the two plant species were reflected in corresponding differences in PC concentration. The observed direct relationship between shoot PC concentration and the degree of metal-induced growth inhibition makes the use of PC promising for the purpose tested for.
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  • 55
    ISSN: 1573-5036
    Keywords: anthesis ; flag leaf area ; number of leaves ; phyllochron ; Triticum aestivum ; Triticum turgidum var. durum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Wheat grain yield production in the rain-fed areas is limited by water deficits during crop growth. A greenhouse experiment was conducted during spring 1992 at ICARDA, Tel Hadya, Syria, with eight genotypes representing two Triticum species (Triticum turgidum var. durum and Triticum aestivum L.) under four soil-moisture regimes (95%, 75%, 55%, and 35% field capacity) to study the effect of water deficit on leaf development. The phyllochron was similar in the two species across the watering regimes. The range in variation in phyllochron among the genotypes was similar in the two species. Phyllochron response to water stress among genotypes was distinct in the driest regime in both species. Cham 6 (T. aestivum) and Gallareta (T. turgidum var. durum) had similar phyllochron across all moisture regimes whereas in other genotypes phyllochron was higher in the dries regime. Leaf area decreased with increasing moisture stress. Triticum turgidum var. durum genotypes were later in flowering as they had, on average, one leaf more than Triticum aestivum genotypes with similar leaf appearance rates.
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  • 56
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    Plant and soil 172 (1995), S. 167-173 
    ISSN: 1573-5036
    Keywords: aluminium ; boron ; copper ; gallium ; iron ; lanthanum ; manganese ; nutrient concentrations ; scandium ; Triticum aestivum ; toxicity ; wheat ; zinc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effects of varying solution concentrations of manganese (Mn), zinc (Zn), copper (Cu), boron (B), iron (Fe), gallium (Ga) and lanthanum (La) on plant chemical concentrations, plant uptake and plant toxicity were determined in wheat (Triticum aestivum L.) grown in a low ionic strength (2.7×10−3 M solution culture). Increasing the solution concentration of Mn, Zn, Cu, B, Fe, Ga and La increased plant concentrations of that ion. Asymptotic maximum plant concentrations were reached for Zn (10 mg kg DM−1 in the roots), Ga (2 mg kg DM−1 in the tops and 18 mg kg DM−1 in the roots) and La (0.4 mg kg DM−1 in the tops and 4 mg kg DM−1 in the roots). Plant ion concentrations were, on average, 3 times higher in the roots than the tops for Mn and Zn, 7 times for Cu, 9 times for Fe, 12 times for Ga and 15 times for La. In contrast, B concentrations were higher in the tops than the roots by, on average, 2 times. The estimated toxicity threshold (plant concentration at which a rapid decrease in yield occurred) in the tops was 0.4 mg g DM−1 for B, 2 for Zn, 0.075 for Cu and 0.09 for La and in the roots 0.2 mg g DM−1 for B, 5 for Zn, 0.3 for Cu and 3 for La. Plant uptake rates of the ions (as estimated by the slope of the relationship between solution ion concentrations and plant ion concentrations) was in the order B〈Fe〈Mn〈La〈Zn〈Ga〈Cu for the tops and B〈Mn〈Fe〈Zn〈La〈Cu〈Ga for the roots. In the roots, the uptake rates of La, Cu and Ga was exceptionally high (〉 250 mg kg DM−1 μM −1). Plant toxicity was estimated as the reciprocal of the plant concentration that reduced yield by 50% (change in relative yield per mg ion kg DM−1). The plant toxicity of the ions tested was in the order Mn〈Zn〈B〈Fe=Ga〈La〈Cu in the tops and Mn〈Ga〈Zn〈Fe=La〈Cu〈B in the roots. Copper was unusual in that plant uptake and plant toxicity was high for a plant trace nutrient.
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  • 57
    ISSN: 1573-5036
    Keywords: Aspergillus fumigatus ; Glomus mosseae ; organic phosphorus utilisation ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In a pot experiment, wheat was grown for 50 days in two heat-sterilized low-phosphorus (P) soils supplied with organic P as Na-phytate. Seed inoculation with the phosphatase-producing fungus (PPF) Aspergillus fumigatus or soil inoculation with the vesicular-arbuscular mycorrhizal (VAM) fungus Glomus mosseae increased shoot and root dry weight and root length, phosphatase activity in the rhizosphere and shoot concentrations of P and to a lesser extent of K and Mg. As a rule, the greatest effects on those parameters were most in the combined inoculation treatment (PPF + VAM). Shoot concentrations of Cu and Zn were only enhanced by VAM, not by PPF. At harvest, depletion of organic P in the rhizosphere soil increased in the order of: sterilized soil 〈 PPF 〈 VAM 〈 PPF + VAM which corresponded with the enhanced P concentrations in the plants. The results demonstrate that organic P in form of Na-Phytate is efficiently used by VAM and that use of organic P can be increased by simultaneous inoculation with phosphatase-producing fungi.
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  • 58
    ISSN: 1573-5036
    Keywords: grain protein concentration ; nitrate ; nitrogen ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Spring wheat (Triticum aestivum L.) was grown with daily additions of nitrate-N. The relative addition rate of nitrate-N was decreased stepwise, and after 125 days of growth, 58 mg N plant-1 had been introduced. The fate and effect of an extra addition of nitrate (20 mg N plant-1) at six different times during the ontogeny (37, 54, 66, 79, 94 and 108 days from sowing) on grain yield and grain protein concentration was investigated. The plants absorbed all or most of the extra nitrate at all stages of development evaluated. Dry matter production of both aerial vegetative parts and grains, but not roots, generally increased as a result of the extra nitrate addition. The increase in grain dry matter was mainly an effect of an increased number of grains per plant. Extra nitrate applications had large effects on grain nitrogen content at all stages, but the effect on main shoot and tiller ears varied depending on the time of application. Early applications, i.e. before anthesis, mainly led to increased yield with unchanged protein concentration whereas late applications also led to increased grain protein concentration. The largest effect on grain nitrogen concentration (25–30% increase) was obtained when the extra nitrate was applied late after sowing, i.e. less than four weeks before final harvest. As the extra dose of nitrate was labelled with 15N, it was possible to follow the movement of the extra nitrogen addition within the plant. Samples were taken at one and five days after 15N-addition and at final harvest. There were differences in the movement of 15N depending on when it was introduced. Generally, net movement of the 15N-labelled N into the grain increased with age at application until 94 days after sowing when a maximum of 90% of the added 15N ended up in the grain.
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  • 59
    ISSN: 1573-5036
    Keywords: C3 ; C4 ; relative growth rate ; Tragus racemosus ; Triticum aestivum ; water stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Influence of short-term water stress on plant growth and leaf gas exchange was studied simultaneously in a growth chamber experiment using two annual grass species differing in photosynthetic pathway type, plant architecture and phenology:Triticum aestivum L. cv. Katya-A-1 (C3, a drought resistant wheat cultivar of erect growth) andTragus racemosus (L.) All. (C4, a prostrate weed of warm semiarid areas). At the leaf level, gas exchange rates declined with decreasing soil water potential for both species in such a way that instantaneous photosynthetic water use efficiency (PWUE, mmol CO2 assimilated per mol H2O transpired) increased. At adequate water supply, the C4 grass showed much lower stomatal conductance and higher PWUE than the C3 species, but this difference disappeared at severe water stress when leaf gas exchange rates were similarly reduced for both species. However, by using soil water more sparingly, the C4 species was able to assimilate under non-stressful conditions for a longer time than the C3 wheat did. At the whole-plant level, decreasing water availability substantially reduced the relative growth rate (RGR) ofT. aestivum, while biomass partitioning changed in favour of root growth, so that the plant could exploit the limiting water resource more efficiently. The change in partitioning preceded the overall reduction of RGR and it was associated with increased biomass allocation to roots and less to leaves, as well as with a decrease in specific leaf area. Water saving byT. racemosus sufficiently postponed water stress effects on plant growth occurring only as a moderate reduction in leaf area enlargement. For unstressed vegetative plants, relative growth rate of the C4 T. racemosus was only slightly higher than that of the C3 T. aestivum, though it was achieved at a much lower water cost. The lack of difference in RGR was probably due to growth conditions being relatively suboptimal for the C4 plant and also to a relatively large investment in stem tissues by the C4 T. racemosus. Only 10% of the plant biomass was allocated to roots in the C4 species while this was more than 30% for the C3 wheat cultivar. These results emphasize the importance of water saving and high WUE of C4 plants in maintaining growth under moderate water stress in comparison with C3 species.
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  • 60
    ISSN: 1573-5036
    Keywords: Gaeumannomyces graminis var. tritici ; manganese ; microorganisms ; nutrient deficiency ; rhizosphere ; roots ; Triticum aestivum ; zinc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The relationship between micronutrient efficiency of four wheat (Triticum aestivum L.) genotypes, tolerance to take-all disease (caused by Gaeumannomyces graminis (Sacc.) Arx and Olivier var. tritici Walker), and bacterial populations in the rhizosphere was tested in soil fertilized differentially with Zn and Mn. Plant growth was reduced by Mn or Zn deficiency and also by take-all. There was an inverse relationship between micronutrient efficiency of wheat genotypes when grown in deficient soils and the length of take-all lesions on roots (efficient genotypes had shorter lesions than inefficient ones). In comparison to the rhizosphere of control plants of genotypes Aroona and C8MM receiving sufficient Mn and Zn, the total numbers of bacterial cfu (colony forming units) were greater in the rhizosphere of Zn-efficient genotype Aroona under Zn deficiency and in Mn-efficient genotype C8MM under Mn deficiency. These effects were not observed in other genotypes. Take-all decreased the number of bacterial cfu in the rhizosphere of fully-fertilized plants but not of those subjected to either Mn or Zn deficiency. In contrast, the Zn deficiency treatment acted synergistically with take-all to increase the number of fluorescent pseudomonads in the rhizosphere. Although numbers of Mn-oxidising and Mn-reducing bacteria were generally low, take-all disease increased the number of Mn reducers in the rhizosphere of Mn-efficient genotypes Aroona and C8MM. Under Mn-deficiency conditions, the number of Mn reducers in the rhizosphere increased in Aroona but not in C8MM wheat. The results suggest that bacterial microflora may play a role in the expression of Mn and Zn efficiency and tolerance to take-all in some wheat genotypes.
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  • 61
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Puccinia recondita ; leaf rust ; breeding ; resistance ; yield ; yield components
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The most important breeding objectives in crop improvement are improving grain yield, grain quality, and resistances to various biotic and abiotic stresses. The objectives of our study were to compare two crossing and four selection schemes for grain yield, yield traits, and slow rusting resistance to leaf rust (Puccinia recondita) based on additive genes in wheat (Triticum aestivum), and to identify the most efficient crossing and selection methodologies in terms of genetic gains and cost efficiency. Segregating populations were derived from 18 simple crosses and the same number of top (three-way) crosses. Half of the crosses were derived from Yecora 70 and the other half from Veery #10 as the common leaf rust susceptible parents. The four selection schemes were: pedigree, modified bulk (F2 and F1-top as pedigree, selected lines in F3, F4, F2-top, F3-top as bulk; and pedigree in F5 and F4-top populations), selected bulk (selected plants in F2, F3, F4, F1-top, F2-top and F3-top as bulk; and pedigree in F5 and F4-top populations), and nonselected bulk (bulk in F2, F3, F4, F1-top, F2-top and F3-top; and pedigree in F5 and F4-top populations). A total of 320 progeny lines, parents and checks were tested for grain yield, other agronomic traits and leaf rust resistance during the 1992/93 and 1993/94 seasons in Ciudad Obregon (Sonora State, Mexico) which represents a typical high yielding irrigated site. The influence of the type of cross and the selection scheme on the mean grain yield and other traits of the progenies was minimal. The selection of parents was the most important feature in imparting yield potential and other favourable agronomic traits. Moreover, the highest yielding lines were distributed equally. Progeny lines derived from Veery #10 crosses had significantly higher mean grain yield compared to those derived from the Yecora 70 crosses. Furthermore, a large proportion of the highest yielding lines also originated from Veery #10 crosses. Mean leaf rust severity of the top cross progenies was lower than that of the simple cross progenies possibly because two parents contributed resistance to top cross progenies. Mean leaf rust severity of the nonselected bulk derivatives was twice that of lines derived from the other three schemes. Selected bulk appears to be the most attractive selection scheme in terms of genetic gains and cost efficiency.
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  • 62
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    Euphytica 100 (1998), S. 253-259 
    ISSN: 1573-5060
    Keywords: hexaploid wheat ; pearl millett ; Pennisetum glaucum ; polyhaploid ; pollen storage ; Triticum aestivum ; wide crosses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effects of drying and freezing on viability of pearl millet pollen were examined with the aim of using stored pollen in polyhaploid production of hexaploid wheat. Freshly collected pollen of pearl millet line NEC 7006 with 55% water content, germinated at a frequency of 80%. Pollen that was dried for two hours to 6% water content showed 50% germination frequency and maintained similar frequencies after the freezing process. In crosses of hexaploid wheat variety Norin 61 with fresh pearl millet pollen, embryos were obtained at a frequency of 27.6%. In crosses with pollen stored at -196 °C, -80 °C and -20 °C for one month, embryo formation frequencies ranged from 27.5 to 17.4%. After five and twelve months of storage, the frequencies ranged from 29.7 to 14.6% at storage temperatures of -196 °C and -80 °C, and from 8.0 to 3.2% at -20 °C, indicating significant differences among storage temperatures. However, no significant frequency difference was found among pollen water contents at the time of collection. All plants regenerated from crosses with pearl millet pollen stored for five months were wheat polyhaploids. These results suggest that stored pearl millet pollen is an efficient medium for producing polyhaploids in hexaploid wheat.
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  • 63
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    Euphytica 100 (1998), S. 305-311 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; winter wheat ; genetic diversity ; ancestors ; genetic profile
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract With the aid of GRIS, the wheat genetic resources database, the retrospective analysis of winter wheat breeding programs in the ex-USSR during its existence, and modern Russia, was conducted. The dynamics of genetic diversity of released cultivars was studied. A significant tendency to reduce the use of local materials was discovered, although a stable set of original ancestors has prevailed over the last 40 years. The modern cultivar genes pool has increased as a result of the utilisation of North American semidwarf varieties. Breeding programs at different breeding centres are distinguished by varying levels of genetic diversity. The need to discover new sources of disease resistance and environmental adaptation are problems that remain.
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  • 64
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    Euphytica 108 (1999), S. 91-95 
    ISSN: 1573-5060
    Keywords: amylose content ; granule-bound starch synthase ; null alleles ; Triticum aestivum ; Wx loci
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The Wx locus controls amylose synthesis in the cereal endosperm. Hexaploid wheat (Triticum aestivum L.) has the three Wx loci on chromosomes 7A ( Wx-A1), 4A (Wx-B1) and 7D (Wx-D1). To verify the effects of null alleles on reducing amylose content and determine the amylose synthesis capacity of each Wx gene independently and accurately, we produced eight possible types of recombinant lines carrying different null alleles at the Wx loci under the ‘Chinese Spring’ genetic background. Amylose content varied from 0% of the waxy ‘Chinese Spring’ to 25% of the ‘Chinese Spring’ normal type. The reducing effect of the single null alleles was the largest in Wx-B1b, and there was no significant difference between Wx-A1b and Wx-D1b. More than 3% reductions in amylose content were detected in the double null types. The results of the double null lines further demonstrated that for the capacity of amylose synthesis, Wx-B1a predominates and produces 21–22% amylose, followed by Wx-D1a (20–21%) and Wx-A1a (15–18%). These significant differences were partly correlated with variation in the amounts of the Wx proteins produced by different Wx genes. However, comparisons of the double null lines with the single null or normal lines indicated that amylose content was not linearly proportional to the number of the Wx genes, suggesting that the Wx genes act in an epistatic manner.
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  • 65
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; gene action ; heritability ; wheat ; tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Estimates of gene actions were obtained for five in vitro traits of immature wheat (Triticum aestivum L.) embryo cultures from a cross of two wheat cultivars and the resulting reciprocal, F1, F2 and backcross populations. The contribution of additive gene effects to in vitro traits was not as important as the dominance gene effects. Epistatic gene effects were relatively more important than either additive or dominance gene effects. Of the individual types of digenic epistatic effects, the dominance x dominance estimates were relatively larger in magnitude for all in vitro culture traits measured. The maternal effect played a minor role in the inheritance of the in vitro studied traits since the difference among the reciprocal values was not significant. It is shown from the generation mean method that epistasis played a major role in the inheritance of most of the traits under study. The negative values of additive and dominance genetic variance were estimates of zero. Heritability estimates, in broad sense, were relatively high for the in vitro studied traits. In some cases, heritability estimates in broad and narrow senses are almost equal since the estimation of dominance genetic variance led to negative values. According to the results of the gene effects, dominance and epistasis were important for the shoot formation trait. Selection would be effective among the isolated genotypes on individual basis.
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  • 66
    ISSN: 1573-5060
    Keywords: Secale cereale ; Triticum aestivum ; yield components ; 1AL/1RS translocation ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effect of the 1AL/1RS chromosome translocation on grain yield and other agronomic characteristics of 85 random F2-derived F6 bulks from three 1AL 1RS × 1A bread wheat crosses was determined under optimum and reduced irrigation conditions at CIANO, Yaqui Valley, Sonora, Mexico, during the 1991–1992 and 1992–1993 crop production cycles. Harvest plots of 5.0 m2 were arranged in an alpha lattice design with three replications. The 1AL/1RS translocation increased grain yield, above-ground biomass, spikes/m2, and test weight under both irrigated and dryland conditions. Homozygous chromosome 1A lines, on the other hand, possessed longer spikes with more grains. The 1AL/1RS cultivars had an advantage in 1000-grain weight, which was detected only under optimum irrigation. The translocation lines showed later maturity and longer grainfilling period than the 1A genotypes under one irrigation treatment. A significant relationship between grain yield and test weight was detected only among the 1AL/1RS genotypes, indicating that they possess heavier and plumper grains than the 1A genotypes. These results encourage the continued use of the 1AL/1RS translocation in wheat improvement.
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  • 67
    ISSN: 1573-5060
    Keywords: infection frequency ; latent period ; septoria tritici blotch ; septoria nodorum blotch ; sporulation rate ; Triticum aestivum ; bread wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Infection frequency (IF), latent period (LP) and sporulation rate (S) were assessed on 23 genotypes (cultivars, breeding lines and parents) in the field and 36 genotypes in the glasshouse (with 22 being in common) following inoculation with conidia of either Mycosphaerella graminicola or Phaeosphaeria nodorum. Significant variation was observed among genotypes for each resistance component. Genotypes resistant to Mycosphaerella graminicola had long LP and low S but variable IF. Genotypes resistant to Phaeosphaeria nodorum had low IF, long LP and low to moderate S. IF and S were independent while a negative correlation was observed between LP and S for Mycosphaerella graminicola only. LP was most consistently correlated with field response. Components measured in the field better discriminated resistant genotypes from others. Combinations of genotypes were sought for which one genotype may contribute to a reduced disease severity for S. nodorum and the other genotype may contribute to a reduced disease severity for S. tritici in mixtures. Such combinations of genotypes were rare.
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  • 68
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; Hordeum vulgare ; wheat ; barley ; chromosome addition lines ; translocation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Two disomic barley chromosome addition lines and five translocated chromosome addition lines of common wheat cultivar Shinchunaga were isolated. They were derived from a hybrid plant between Shinchunaga and cultivated barley Nyugoruden (New Golden) by backcrossing with wheat and self pollination. Barley chromosomes added to chromosome arms involved in the translocated chromosomes were identified by C-banding method and by crossing these lines with Chinese Spring/Betzes addition lines. Two disomic addition lines were identified to have chromosome 6 and 7 of barley, respectively. Two of the five translocated chromosome addition lines were clarified to have same chromosome constitution, 42 wheat chromosomes and a pair of translocated chromosomes constituted with a long arm of chromosome 5B of wheat and a short arm of chromosome 7 of barley. The other three lines could not be identified due to chromosome rearrangement. Performances of these seven lines on agronomic characters were examined. Addition of barley chromosome 7 induced early heading, and chromosome 6 showed lated heading. Almost all of the lines except that of chromosome 6 showed short culm length and all showed reduced number of tillers, spikelets and grains per ear, and low seed fertility. These lines would be useful for genetic analyses in wheat and barley and for induction of useful genes of barley into wheat.
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  • 69
    ISSN: 1573-5060
    Keywords: clustering procedure ; genotype × environment interaction ; multi-trait analysis ; quality ; wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Structure of genotype × environment interaction was studied in two series of trials for three quality traits in bread wheat. Two kinds of environments were present in each series of trials: macro-environments defined as locations or location × year combinations and micro-environments induced by diversified cultural practices within each site. For each trait, a simultaneous clustering procedure was used to identify groups of environments which were homogeneous for interaction. An optimised series of trials was proposed from the clusters obtained. The cultural practice based on nitrogen fertilisation seemed to better diversify environments for interaction than use of fungicide, when all quality traits were considered. Determining an optimised series of trials simultaneously for the three traits led to keeping more environments than when one trait was considered. Suggestions for establishing a series of trials for a multi-trait analysis were proposed.
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  • 70
    ISSN: 1573-5060
    Keywords: in situ hybridization ; Psathyrostachys juncea ; chromosome translocation ; translocation addition ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Using the genomic in situ hybridization (GISH) technique, one translocation line, seven translocation-addition lines, five translocation plus translocation addition lines and two ditelosomic addition lines were identified in backcross progenies of Triticum aestivum L. -Psathyrostachys juncea (Fisch.) Nevski intergeneric hybrids. No complete P. juncea chromosomes were detected in the 25 lines studied. The results suggest that intact P. juncea chromosomes may be difficult to isolate in a wheat background.
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  • 71
    ISSN: 1573-5060
    Keywords: embryogenesis ; wheat ; maize ; Triticum aestivum ; Zea mays ; haploidy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Embryogenesis was analyzed in wheat × maize hybrids using paraffin sectioning. Embryogenesis in wheat × maize hybrids is different from that in self-pollinated wheat plants. Development of the embryo is not accompanied by the formation of an endosperm. The endosperm nuclei remain free in the cytoplasm, fail to advance into the cellular stage, and degenerate at a later time. The antipodal cells quickly degenerate in the fertilized ovaries of wheat × maize hybrids similar to self-pollinated ovaries. The antipodal cells remain normal in unpollinated ovaries. The pre-embryo will abort if it is allowed to develop on the plant, because of a nutritional shortage in the absence of an endosperm. Therefore, embryo rescue is necessary for haploid production from a wheat × maize hybrids. Haploid polyembryos were obtained from spikelet culture of wheat × maize hybrids. The formation of polyembryos is due to the cleavage of the pre-embryo and the effect of 2,4-D. The frequency of haploid embryo production and plant regeneration is affected significantly by maize genotypes, but not by wheat genotypes. The concentration of 2,4-D affects only the size of the embryo.
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  • 72
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; X Triticosecale ; interspecific hybrids ; virus resistance ; BYDV ; Russian wheat aphid ; Diuraphis noxia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The Barley Yellow Dwarf Virus disease (BYDV) and the Russian wheat aphid (RWA) Diuraphis noxia (Mordvilko) have caused significant losses to wheat and barley in several areas of the world. Important sources of resistance to both BYDV and RWA have been found in Triticale. Different generations of interspecific wheat x Triticale crosses were produced and the progenies were screened for BYDV and RWA tolerance. Plants with equal chromosome numbers showed different levels of fertility. A significant correlation was observed between pollen fertility and seed set in primary florets (r=0.57). In generaL, pollen fertility, seed set and the number of euploid plants (2n=42) increased from one generation to the next. The expression of BYDV tolerance varied from population to population. Additive effects were predominant in F1 and some backcross populations. A dominant effect of rye tolerance genes was also observed in few populations. A monogenic trait or a quantitative (polygenic) character would not agree with the observed segregation patterns. The heritability of this oligogenic tolerance was quite different between populations and in many populations the tolerance genes were only partially expressed. Some transgressive segregation for tolerance and sensitivity was demonstrated. The genes controlling tolerance to RWA in Triticale lines, Muskox 658 and Nord Kivu were not expressed in advanced lines resistant to BYDV. This indicates that tolerance genes for BYDV and RWA in these lines are located on different chromosomes.
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  • 73
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    Euphytica 92 (1996), S. 95-103 
    ISSN: 1573-5060
    Keywords: rusts ; virulence genes ; wheat ; resistance genes ; Czech Republic ; Slovak Republic ; Triticum aestivum ; Thalictrum species
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In former Czechoslovakia virulence of rusts attacking wheat was studied since the sixties. Since the same time genes for resistance in the registered cultivars were identified. The role of Berberis and Thalictrum as alternate hosts for stem rust and leaf rust, respectively, was investigated as well. Determined changes of virulence in rust populations could only partially be ascribed to changes of resistance genes in the grown cultivars. Unnecessary genes for virulence had no negative effect on the fitness of the pathogen. All tested samples of aeciospores from barberries attacked rye, not wheat. None of Thalictrum species occurring in the Czech and Slovak Republics was found to host wheat leaf rust. However, the sexual stage of wheat stem rust and wheat leaf rust could be induced on Berberis vulgaris and Thalictrum speciosissimum, respectively. General epidemiological conclusions are drawn from the results and experience of the last 35 years.
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  • 74
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    Euphytica 93 (1997), S. 353-360 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; Secale cereale ; powdery mildew ; Erysiphe graminis f.sp. tritici ; resistance ; suppression ; segregation distortion ; wheat ; rye
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The location of wheat gene Pm8 for resistance to powdery mildew in the 1RS chromosome segment derived from cereal rye cultivar Petkus was confirmed. There was reduced transmission of the 1BL.1RS chromosome relative to its wheat homologue in four of the five crosses examined. Pm8 was not expressed in some wheat cultivars and selections which possessed the 1RS chromosome identified by the presence of relevant genes for resistance to the three rusts, due to the presence of a dominant suppressor gene(s) in the wheat genome. The frequency of suppression in 1BL.1RS wheats from Mexico was significantly higher than in a group of wheats (both local and exotic) introduced from China and was probably much higher than in European wheats.
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  • 75
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    Euphytica 94 (1997), S. 119-124 
    ISSN: 1573-5060
    Keywords: glume tenacity ; inheritance ; rachis fragility ; spelta ; semi-wild wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The inheritance of rachis fragility and glume tenacity in semi-wild wheat was studied in an attempt to help establish the taxonomic status and genetic origin of semi-wild wheat. Progenies of crosses and backcrosses of semi-wild wheat with the cultivar Columbus (common wheat) indicated that the fragile rachis and non-free-threshing character of semi-wild wheat were dominant to the tough rachis and free-threshing character of common wheat. F2 and backcross data indicated that the rachis fragility and glume tenacity of semi-wild wheat were each controlled by a single gene in the cross of semi-wild wheat with Columbus. In the cross of semi-wild wheat with Triticum aestivum spp. spelta, the F2 and F3 population did not segregate for glume tenacity, but did segregate for rachis fragility. The F2 and backcross data suggest that three genes interact to control three types of rachis fragility, i.e. semi-wild wheat-type, spelta-type and the tough rachis of common wheat. Semi-wild wheat differs from common wheat in rachis fragility and glume tenacity. This wheat also differs from other wheats with fragile rachis and tenacious glumes (T. aestivum ssp. spelta, macha and vavilovii) in the pattern and degree of rachis disarticulation. We conclude that semi-wild wheat is likely a subspecies within T. aestivum at the same taxonomic level as spp. spelta, macha and vavilovii.
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  • 76
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    Euphytica 94 (1997), S. 303-309 
    ISSN: 1573-5060
    Keywords: photosynthesis ; hexaploid wheat ; A genome ; D genome ; polyploidy ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract We assessed (1) the effects of addition and doses of the D genome from different sources and (2) the addition of either the A genome or the D genome on the photosynthesis of synthesized hexaploid wheats. On average, the increased doses of the D genome reduced photosynthesis, but the depression was dependent on the source of the D genome. Two accessions of Aegilops squarrosa had depressed photosynthetic rates, but not another accession of Ae. squarrosa. The D genome of cv. Thatcher did not contribute to depress photosynthetic rate. Triticum monococcum had considerably higher photosynthetic rates than Ae. squarrosa. However, addition of the A genome from T. monococcum did not increase the photosynthetic rates of hexaploids. Chlorophyll a : b ratio, functional photosystem II and the core complex of photosystem II did not account for the variation in photosynthetic rate among the genotypes studied. In our experiment, photosynthesis of polyploids was not dependent on photosynthesis rates of the donor genomes.
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  • 77
    ISSN: 1573-5060
    Keywords: Boron ; genotypic variability ; sterility ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Two field experiments were carried out at the Khairanitar Farm, Nepal during the winter season of 1993 to investigate whether wheat genotypes differ in their sterility response to low soil boron and whether boron added to soil can correct sterility. A nursery consisting of 41 diverse genotypes from Nepal, India, Bangladesh, Thailand, China, Pakistan and CIMMYT/Mexico were evaluated in a plot where severe sterility had been observed in previous years. The second experiment involved six genotypes of known sterility responses to boron grown with or without an application of boron at 1 kg ha-1. Large differences were observed among genotypes in terms of number of grain set per ear (range 0.5 to 30 ) and sterility (5.5% to 97.5%). About one third of all the genotypes had 〈25% sterility and 16 of them suffered 〉75% sterility. The genotypes Fang-60, Sonalika, De Mai 6-22, BUC/FLK/MYNA/VL and HDW-234 were highly tolerant to boron deficiency with 〈10% sterility. In sharp contrast, genotypes BOW/BUT, SERI/THB, Glennson, SW-41, Yunmai-33 and UHU were highly susceptible and set virtually no grains in the boron deficient plots. Strong responses to boron application were observed and genotypic variations were evident. Boron at 1 kg ha-1significantly reduced the number of late ears, increased number of grains per ear and grain yield in boron-responsive genotypes. Tolerant genotypes were not influenced by added boron. Given these striking genetic differences, progress in breeding wheat for B-deficient soils in Nepal should be worthwhile.
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  • 78
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    Plant growth regulation 28 (1999), S. 129-132 
    ISSN: 1573-5087
    Keywords: dormancy ; Lactuca sativa ; lettuce seeds ; Brassica napus ; rapeseed ; combustion products ; Salix viminalis ; Themeda triandra ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In darkness, dormancy was imposed on seeds of lettuce (Lactuca sativa L. cv. Grand rapids) by high temperature and on seeds of oilseed rape (Brassica napus L. cv. Apex) by osmotic stress using polyethylene glycol (PEG 8000). In both cases, dormancy was broken by incubating the seeds in aqueous extracts of combustion products from Salix viminalis wood chips or Themeda triandra leaves. Dormancy of rapeseed, but not lettuce, was also broken by a solution of smoke from burnt straw of Triticum aestivum. The greatest stimulation from burnt vegetation was achieved with an aqueous extract of pyrolysed willow wood chips, which had been subjected to temperatures of up to 800 °C during combustion in a down-draught gasifier. This suggests that some biologically active substances obtained from combustion of plant tissues are highly heat-stable.
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  • 79
    ISSN: 1573-5087
    Keywords: cortical fibre cell ; gibberellic acid ; spring wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The role of gibberellic acid (GA) in differentiation and secondary cell-wall deposition of fibre cells of spring wheat (Triticum aestivum) culms was studied using applications of GA and chlormequat (a GA biosynthesis inhibitor). In certain genotypes, higher GA levels may increase the number of cortical fibre cell files by changing cell fate from parenchyma to fibre, and induce thicker secondary cell-walls.
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  • 80
    ISSN: 1573-5087
    Keywords: Triticum aestivum ; water stress ; ethylene ; antioxidant enzymes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In this work it has been found that ethylene production increased only slightly under conditions of a moderate or severe water stress. However, the rehydration of the plants at full turgor after desiccation caused a high emission of ethylene. The desiccation would not irreversibly inactivate the enzymes of the ethylene pathway, since rehydration made the synthesis recommence almost immediately. Water deficit also increased the free radical levels and the antioxidant scavengers, such as superoxide dismutase. Free radicals promote the conversion of 1-amino-cyclopropane-1-carboxylic acid to ethylene, then it is logical to think that both chemical species are involved in the phenomenon of the acceleration of the grain maturity before the plant collapses.
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  • 81
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    Genetic resources and crop evolution 46 (1999), S. 469-475 
    ISSN: 1573-5109
    Keywords: foliar blight ; germplasm ; resistance ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract One thousand three hundred and eighty-seven spring wheat germplasm (Triticum aestivum L.) lines belonging to the Indian and CIMMYT wheat programmes were evaluated for their tolerance to foliar blight disease for three consecutive years i.e., from 1994 to 1997. Disease severity at six different growth stages, beginning from tillering to late milk stage, was recorded. None of the genotypes showed immunity to the disease. Of 43 lines showing resistant reaction, a major proportion (25) was represented by CIMMYT material. Comparatively, Indian germplasm lines tended to be more susceptible at more advanced growth stages. Area Under Disease Progress Curve (AUDPC) and Apparent Infection Rate (r) values of resistant lines were much lower than those of susceptible ones, but lower AUDPC in some of the resistant lines did not correspond to a lower 'r' value. Most of the resistant lines were derived from Seri, Myna, Bau, kauz, Hork 's' and Aegilops tauschii Coss.
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  • 82
    ISSN: 1573-0867
    Keywords: bicarbonate-extractable soil phosphorus ; Lupinus angustifolius ; residual value ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Decreases in Colwell bicarbonate soil test P in the years after applying single (ordinary) superphosphate, and the residual value of superphosphate, was measured in a long-term field experiment on a duplex (texture contrast) soil (sand over lateritic ironstone gravel clay sand at 10–15 cm), at Wongan Hills, Western Australia, typical of many soils used to grow crops in Western Australia. Ten levels of P (0–91 kg P ha-1) were applied once only in late May to different plots in different years from 1988 to 1993. Wheat (Triticum aestivum), or lupin ( Lupinus angustifolius)) were sown in late May of each year, when the P treatments applied that year were banded (drilled) with the seed. Soil samples were collected each June to measure soil test P. Seed (grain) yields of the crops were measured each December. The residual value (RV) of P applied in previous years was calculated relative to P applied in the current year, using grain yields (RVyield) and soil test P (RVsoil). Soil test P measured on soil samples collected in June was related to yields measured in December that year to provide soil P test calibrations. Relative to P applied in the current year, soil test P decreased by between 15 to 30% for P applied one year previously, by 25 to 30% for P applied three years previously, and by 60 to 70% for P applied six years previously. Soil test P was affected by spatial variation, and it also varied in the different years, for P applied in the current year, one year previously, two years previously, etc. Compared with P applied in the current year, mean RVyield determined in the different years decreased by about 40% one year after P application, followed by a further 20% decrease for P applied two years previously, followed by a further 20% decrease for P applied three to five years previously. Relative to current P, RVsoil decreased by about 25% one year after P application, followed by a further 20% for P applied two years previously, followed by a further 10% for P applied three years ago, and followed by a further 6% for P applied four and five years ago. As measured in the different years, the soil P test calibration varied between years for P applied one, two etc. years previously. This was so even when the same cultivar of wheat was grown at the same site in different years.
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  • 83
    ISSN: 1573-5087
    Keywords: flooding ; kinetin ; leaf relative water content ; membrane stability ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Wheat plants, 22d. old, were exposed to wide range of soil water osmotic potential (Ψs = 0 to −1.2 MPa) induced by NaCl and CaCl2 treatments in combination with roots maintained under aerobic (drained at field capacity) or nonaerobic (flooded) conditions in the soil, and sprayed with 10 mg L−1 kinetin solution. In drained plants, not receiving kinetin, increased soil salinity resulted in appreciable inhibition of shoot growth and reduction in chlorophyll (Ch1.), soluble sugars (SS) contents and grain yield. Shoot growth, Ch1. content, soluble sugars and grain yield were significantly lower for flooded plants than unflooded analogues over the entire Ψs range. Both salinity and waterlogging synergize to increase Na+, Ca+ and Cl− accumulation in shoot tissues and to decrease the stability of leaf membranes to either dehydration (40% polyethylene glycol 6000) or heat (51 °C) stress. The ratio of K+/Na+ transported to shoots under aerobic and anaerobic conditions decreased progressively on salinization. The association between the internal mineral element concentrations was largely affected by kinetin treatment. Kinetin application ameliorated the deleterious effects of salinity and oxygen deficiency. It reduced Na+, Ca2+ and Cl− accumulation and improved K+ uptake under salinity and waterlogging stresses. Increased K+/Na+ ratio helped the plants to avoid Na+ toxicity and enhanced shoot growth and grain yield. Kinetin also reduced membrane injury by dehydration and heat stresses and improved the water status of plants under both aerobic and anaerobic conditions. The effects of single factors (Soil salinity ‘Ψs’, soil waterlogging ‘WL’ and Kinetin ‘Kin’) and their interactions (Ψs × WL, Ψs × Kin, WL × Kin and Ψs × WL × Kin) were shown by analysis of variance to be statistically significant for most parameters tested. Calculation of the coefficient of determination (η+) led to three important findings. (1) Salinity (Ψs) was dominant in affecting leaf relative water content (RWC), shoot dry mass, grain yield, stability of leaf membranes to dehydration stress and the contents of Na+, Ca2+, Mg2+ and Cl−. (2) Kinetin (Kin) had a dominant effect on the stability of leaf membranes to heat stress as well as on chlorophyll and soluble sugars contents. (3) The share of waterlogging (WL) was dominant for K+ content. It can be concluded that kinetin application helped wheat plants to grow successfully in the areas subjected to combined effects of salinity and oxygen deficiency, such as in salt marshes.
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  • 84
    ISSN: 1573-5109
    Keywords: Aegilops tauschii ; hairy auricles ; hairy leaf sheath ; inheritance ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Inheritance of genes for hairy auricles and hairy leaf sheath of Ae. tauschii in hexaploid wheat backgrounds (synthetic hexaploid wheat and common wheat varieties) was analyzed. The results indicated that hairy auricles and hairy leaf sheath of Ae. tauschii can be transferred and are expressed in hexaploid wheat. In a synthetic hexaploid wheat ('Ae. tauschii' 188) hairy auricles was proved to be controlled by a single dominant gene derived from Ae. tauschii, which was different from the Pa gene located on chromosome 4BS of common wheat. The hairy leaf sheath phenotype of 'Altar 84/Ae. tauschii 188' was also controlled by a single dominant gene derived from Ae. tauschii, which is obviously different from the Hls gene in T. dicoccoides. We suggest to designate the Ae. tauschii genes for hairy auricles and hairy leaf sheath as Pa2 and Hls2, respectively; such genes could be used as useful genetic markers in common wheat.
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  • 85
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; Heterodera avenae ; resistance ; breeding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Breeding wheat for resistance to Heterodera avenae in southern Australia has been in progress for nearly 30 years and recently a number of resistant varieties have been released. Early breeding work was hampered by three factors: • a lack of appreciation of the role and extent of the problem, • inaccurate, slow screening methods, ultimately being replaced by the 'tube' test and soon by linked molecular markers, • inappropriate breeding strategies, so that varietal releases have taken place only when the breeding has been fully integrated into the main programs. The experiences in southern Australia will be relevant to many other areas in the world where H. avenae is the major pest.
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  • 86
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    Euphytica 100 (1998), S. 131-135 
    ISSN: 1573-5060
    Keywords: boron ; tolerance ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A new method for screening wheat for boron tolerance has been developed to overcome the problems of methods used in the past. Seedlings were grown for 10 days while suspended over B-rich solutions. Their root lengths were then measured as an index of tolerance. Five varieties of wheat were screened at seven concentrations of B. Results were obtained more quickly and easily than from the alternative methodologies and compared favourably. Screening of 128 doubled haploid lines from a cross between Halberd and (Wl*MMC) suggested that transgressive segregation had occurred.
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  • 87
    ISSN: 1573-5060
    Keywords: leaf rust ; Puccinia recondita f. sp. tritici ; resistance genes ; Triticum aestivum ; Triticum monococcum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Monosomic analysis was conducted to determine chromosomal locations of three new leaf rust resistance genes recently transferred to common wheat (Triticum aestivum) from T. monococcum. The resistance gene in wheat germplasm line KS92WGRC23 was transferred from T. monococcum ssp. monococcum. The resistance genes found in KS93U3 and KS96WGRC34 were transferred from T. monococcum ssp. aegilopoides. Allelism tests showed that the three resistance genes were unlinked. The three lines were crossed with each of the seven A-genome Wichita monosomic lines. The leaf rust resistance genes in KS92WGRC23, KS93U3, and KS96WGRC34 were located on chromosomes 6A, 1A, and 5A, respectively, by monosomic analysis. These results demonstrate that the three new genes derived from T. monococcum are each different. They also differ from previously reported Lr genes. This information on chromosome location and the development of mapping populations will facilitate molecular tagging of the new genes.
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  • 88
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    Euphytica 102 (1998), S. 219-226 
    ISSN: 1573-5060
    Keywords: antifreeze proteins ; chromosome substitution lines ; cold acclimation ; freezing tolerance ; Triticum aestivum ; winter survival
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Antifreeze proteins (AFPs) accumulate in the leaves of winter cereals during cold acclimation, where they may inhibit ice recrystallization during freezing and thawing cycles and provide nonspecific disease resistance. In this study, 21 wheat chromosome substitution lines and the parental lines Chinese Spring and Cheyenne wheat were used to determine the heritability of AFPs and the relationship between the accumulation of AFPs and winter survival. In cold-acclimated lines, antifreeze activity in leaf apoplastic extracts ranged from 1 (low) to 5 (high) with an average value of 3.2, and the accumulation of apoplastic proteins ranged from 30 μg (g FW)-1 to 115 μg (g FW)-1 with a mean value of 70 μ (g FW)-1. Examination of the individual lines revealed that Cheyenne chromosomes 5B and 5D carry major regulatory genes that increase both antifreeze activity and the accumulation of antifreeze proteins in plants grown at low temperature. Substitution lines carrying Cheyenne chromosomes 2A, 3A, 6B, and 7A exhibited lower freezing tolerance and also showed a marked decrease in the accumulation of specific AFPs during cold acclimation. Antifreeze activity and apoplastic protein content were not correlated with freezing tolerance (defined as % survival at -11 °C), but they were both significantly and positively correlated with winter field survival rates. Antifreeze activity (positively correlated) and total leaf fresh weight (negatively correlated) together accounted for about 55% of the variation in winter survival, indicating that high antifreeze activity and slow vegetative growth at low temperature are both important quantitative traits for winter survival.
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  • 89
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    Euphytica 105 (1999), S. 125-131 
    ISSN: 1573-5060
    Keywords: disease resistance ; inheritance ; Karnal bunt ; Neovossia ; Tilletia indica ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Inheritance of resistance to Neovossia indica was studied in a Triticum aestivum line HD 29. To overcome the influence of environment on disease expression, the study was conducted by extensive evaluation of advanced generation (F8) recombinant inbred lines (RILs) developed by single seed descent from the cross WL 711 (susceptible) × HD 29 (resistant. The results suggested that HD 29 possesses three major genes for resistance to isolated Ni7 and two genes for resistance to isolate Ni8. One of the two genes controlling resistance to Ni8 is common with one of the genes conferring resistance to Ni7. These observations have important implications in breeding for Karnal bunt resistance.
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  • 90
    ISSN: 1573-5060
    Keywords: C-banding ; chromosome additions ; chromosome substitution ; Elymus tsukushiense ; homoeology ; in situ hybridization ; RFLP ; Roegneria kamoji ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Elymus tsukushiense Honda (syn. Roegneria kamoji C. Koch) (2n = 6x = 42, StsStsHtsHtsYtsYts) is a hexaploid species, distantly related to bread wheat Triticum aestivum L. em Thell (2n = 6x = 42, AABBDD). Apart from the delineation of evolutionary relationships, this species is a potential source of resistance to scab, a devastating disease of wheat caused by Fusarium graminearum Schw. A standard C-banded karyotype was established identifying all 21 chromosome pairs of E. tsukushiense. By using C-banding and genomic in situ hybridization analyses, three wheat-E. tsukushiense chromosome addition lines, one ditelosomic addition line, and one disomic substitution line were identified in BC2 progenies from wheat × E. tsukushiense hybrids. Twenty DNA markers specific for the seven homoeologous groups of the Triticeae were used to determine the homoeology of the added E. tsukushiense chromosomes. The E. tsukushiense chromosomes in the addition lines NAU702, NAU703, and NAU701 were identified as belonging to homoeologous groups 1, 3, and 5, and thus, were designated as 1Ets#1, 3Ets#1, and 5Ets#1, respectively. NAU751 was identified as a disomic substitution line with chromosome 3A of wheat replaced by chromosome 3Ets#1. Line NAU702 has a high level of resistance to scab and will be used in chromosomal engineering and development of improved wheat germplasm for scab resistance breeding.
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  • 91
    ISSN: 1573-5060
    Keywords: 1BL/1RS ; bread-making quality ; prolamins ; wheat-rye translocations ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The allelic variation of prolamin loci was studied in three F2 progenies from three crosses between the 1BL/1RS cultivar Triana and Yécora Rojo, Pavón and Florence Aurora, cultivars without the translocation. According to the 1:2:1 theoretical proportions observed in the allelic variants of the Glu-B3/Gli-B1 loci of the parent without the translocation, the inheritance as a block of the rye chromosome arm was confirmed. A group of F3-F4 recombinant lines, developed from these crosses was evaluated using the SDS-sedimentation test and the mixograph and alveograph tests. The presence of the 1BL/1RS translocation was not associated with significantly lower grain protein content values or with the optimum mixing time in the mixograph of the genotypes. The effect of the 1BL/1RS translocation on most of the quality parameters was highly dependent on the genetic pool. Significant increases in gluten strength and better mixing properties associated with the presence of some alleles of the Glu-A1, Glu-A3/ Gli-A1 and Gli-D2 loci were detected. The additivity and the interaction of prolamin gene effects with the rye translocation in the 1BL/1RS lines and its possible use in plant breeding are discussed.
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  • 92
    ISSN: 1573-5060
    Keywords: Blumeria graminis ; powdery mildew ; QTL ; RFLPs ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A segregating population of doubled-haploid lines issued from the cross between the wheat (Triticum aestivum L. em. Thell) cultivars Courtot, resistant to several isolates of powdery mildew (Blumeria graminis DC. f. sp. tritici Em. Marchal), and Chinese Spring (susceptible) was used to map Mlar, a gene carried by Courtot and conferring resistance to this pathogen. The assignation of Mlar using monosomic lines of Courtot was confirmed by the mapping analysis. Mlar was located on the short arm of the chromosome 1A, in the vicinity of the locus XGli-A5 coding for storage proteins. This result was in accordance with those demonstrating that Mlar was an allele of the Pm3 locus (Pm3g), a gene also involved in the resistance to powdery mildew.
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  • 93
    ISSN: 1573-5060
    Keywords: disease assessment ; maturity ; resistance ; Septoria tritici ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Nineteen cultivars, with large differences in heading date, were evaluated for their response to septoria tritici blotch in two experimental setups in Njoro, Kenya. Due to the more or less constant temperatures during the growing season and the overhead irrigation applied the epidemic conditions were similar over the whole observation period for the early and late cultivars. In experiment 1 the cultivars were assessed for disease severity at the same moment irrespective of the developmental stage, while in experiment 2 the cultivars were assessed at the same developmental stage. Measured at the same time, the disease severity was highest in the early maturing cultivars and lowest in the late maturing cultivars (r = –0.78). When assessed at the same development stage the disease build up was independent of heading date (r = –0.10) but strongly dependent on resistance level. There were no indications that early heading cultivars were more susceptible than late heading cultivars.
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  • 94
    ISSN: 1573-5060
    Keywords: allelic variation ; glutenin subunit ; Japanese Norin variety ; seed storage protein ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Seed storage proteins of 131 Japanese Norin wheat (Triticum aestivum) varieties were fractionated by sodium dodecyl sulfate polyacrylamide gel electrophoresis to determine allelic make-up in varieties at each of three loci that control high-molecular-weight (HMW) glutenin subunits. Three alleles were identified at the Glu-A1 locus, six at the Glu-B1 locus and five at the Glu-D1 locus. Twenty-four different, major glutenin HMW subunits were identified and each contained three to five subunits and seventeen different glutenin subunit patterns were observed for 19 subunits in the 131 Japanese Norin varieties. Fourteen alleles were identified by comparison of subunit mobility with that previously found in hexaploid wheat. Japanese Norin varieties showed a specific pattern of allelic variation in glutenin HMW subunits, different from that of Chinese and other country common wheats in allelic frequency at Glu-1 loci.
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  • 95
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    Euphytica 83 (1995), S. 153-157 
    ISSN: 1573-5060
    Keywords: acquired high temperature tolerance ; diallel analysis ; inheritance ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The development of high temperature-tolerant wheat (Triticum aestivum L.) germplasm is necessary to improve plant productivity under high-temperature stress environments. The quantification of high temperature tolerance and the characterization of its genetic control are necessary for germplasm enhancement efforts. This study was conducted to determine the genetic control of acquired high temperature tolerance in common bread wheat cultivars. Reduction of 2,3,5-triphenyltetrazolium chloride (TTC) by heat-stressed seedling leaves was used as a quantitative measure to characterize acquired high temperature tolerance. Eleven-day-old seedlings of 20 F1 progeny produced through a complete 5×5 (‘Payne’, ‘Siouxland’, ‘Sturdy’, ‘TAM W-101’, and ‘TAM 108’) diallel mating design were acclimated at 37° C for 24 hours, followed by a 2-hour incubation at 50° C. Under these test conditions, acquired high temperature tolerance ranged from a high of 75.7% for the genotype TAM W-101 × TAM 108, to a low of 37.3% for the genotype Payne × Siouxland. Partitioning of genotypic variance revealed that only the general combining ability component effect was statistically highly significant, accounting for 67% of the total genotypic variation. These results suggest that enhancing the level of high temperature tolerance in wheat germplasm is feasible utilizing existing levels of genetic variability and exploiting additive genetic effects associated with high temperature tolerance.
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  • 96
    ISSN: 1573-5060
    Keywords: wheat-rye chromosomal translocations ; C-banding ; in situ hybridization ; greenbug resistance ; Triticum aestivum ; Secale cereale ; Schizaphis graminum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary C-banding andin situ hybridization were used to determine the chromosomal constitution of the greenbug-resistant germplasm GRS 1204. The results showed that this line had the radiation-induced non-homoeologous wheat-rye translocation chromosomes T2AS-1RS·1RL and T2AL·2AS-1RS. C-banding analysis further revealed the presence of a wheat-Agropyron elongatum translocation chromosome T1BL·1BS-3Ae#1L in line GRS 1204, that was derived from ‘Teewon’. The greenbug resistance of line GRS 1204 is similar to that of line GRS 1201 that was earlier shown to have the greenbug resistance geneGb6 located on the 1RS arm of the wheat-rye translocation chromosome T1AL·1RS. BecauseGb6 in line GRS 1204 is present on one of the non-homoeologous translocation chromosomes, agronomically line GRS 1201 should be the better adapted source ofGb6 resistance and be used in cultivar improvement.
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  • 97
    ISSN: 1573-5060
    Keywords: bread-wheat ; chromosome deletion ; restriction fragment length polymorphism ; RFLP ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A study was undertaken to evaluate the breeding behaviour and to identify a spontaneously produced putative chromosomal deletion in the winter wheat (Triticum aestivum L. em. Thell.) cv Norstar. Male and female transmission studies of plants heterozygous for the deletion chromosome indicated 9.5% and 48.8% transmission through the pollen and the egg, respectively. Meiotic analyses of progeny from deletion heterozygotes indicated that the deletion chromosome was eliminated from half of the plants, which agreed with the male and female transmission frequencies. Testcrosses of the deletion chromosome with telocentrics and nullisomic-tetrasomic combinations suggested that the deletion involved the long arm of chromosome 5D. This was confirmed by restriction fragment length polymorphism (RFLP) analysis. Also, monosomic plants obtained in progeny of deletion heterozygotes were shown to be monosomic for 5D. Studies of plants homozygous for the deletion showed relatively normal pairing between the deletion chromosomes, and with the short arm (5DS), but not the long arm (5DL). Deletion homozygotes were self-sterile, and morphologically similar to plants nullisomic for 5D, but plants that also contained 5DL, or a homoeologous chromosome were self-fertile and had normal morphology. Studies of chromosome morphology indicated that the deletion chromosome was metacentric, and the length of the long arm was reduced by approximately 60%. RFLP studies showed that, in terms of genetic distance, 90% of the arm was missing.
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  • 98
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    Euphytica 95 (1997), S. 209-219 
    ISSN: 1573-5060
    Keywords: gene expression ; gluten ; glutenin ; protein fingerprint ; Triticum turgidum ; var. dicoccoides ; wheat ; Triticum aestivum ; Triticum durum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Wheat seed storage protein fingerprint is used to determine the gluten protein pattern in studies aimed at improving flour quality. Wild wheat with high seed protein content is used extensively in wheat breeding programs. Although the wild wheat growth and protein content may be influenced by environmental conditions, the gluten-protein pattern is generally considered as indicative of a genotype, without the superimposition of environmental influences. The effects of soil type, habitat, and deficiencies of N, P, K and S on seed storage protein composition were examined in nine accessions of wild wheat (Triticum turgidum var. dicoccoides) and three varieties (two T. aestivum and one T. durum). Soil from ten natural habitats of the wild wheat that had not previously received any fertilizers or manures was sampled and used to grow wheat in a greenhouse. Seed storage protein composition was characterized by SDS-PAGE. Although deficiencies in soil nutrient caused variations in the seed storage proteins, the genotype was the main factor determining the seed storage protein composition. Seed storage protein composition of genotypes varied when grown under different mineral nutrient conditions. Only one genotype was stable showing almost identical protein patterns under all growing conditions studied without any qualitative change in fingerprint pattern. In the other genotypes, as well as the cultivars, the seed storage protein was affected at least to some extent by the soil. The ‘soil effect’ is summarized in terms of three main quantitative changes in the seeds: 1 – the relative amounts of the high-molecular-weight proteins; 2 – the relative amounts of proteins in the range of 45 and 65 kD; 3 – the percentage distribution of the HMW glutenin and other groups of seed storage proteins. The soild induced also qualitative differences in the composition of seed storage proteins, mostly in those of 45–65 kD. These differences were observed whenever a deficiency of S, N, P, K or Mg was identified. Therefore, in breeding programs that use seed storage protein fingerprints of wild wheat germplasms should be exercise caution when the germplasms selected from wild habitats.
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  • 99
    ISSN: 1573-5060
    Keywords: gramineae ; polyembryony ; somatic embryogenesis ; Triticum aestivum ; wheat ; genetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In the course of experiments performed to obtain haploid wheat plants in which 2,4-dichlorophenoxyacetic acid (2,4-D) was applied to developing spikes, it was found that three cultivars showed a different ability to produce polyembryos (Thatcher 20.19%, Chris 7.06%, Dollar 0%). This behaviour was related to their capacity to form somatic embryos. Diploid immature embryos cultured in vitro after 2,4-D treatment, gave a higher frequency of embryogenic callus in Thatcher and Chris than in Dollar. As the common factor in both experiments was the 2,4-D treatment we propose that the three cultivars showed a differential sensitivity to 2,4-D.
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  • 100
    Electronic Resource
    Electronic Resource
    Springer
    Euphytica 96 (1997), S. 257-262 
    ISSN: 1573-5060
    Keywords: boron deficiency ; fertility selective media ; hybridization ; male sterility ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Boron (B) deficiency causes grain set in wheat to fail. A wide range of genotypic variation in the response to low B has been observed. Genotypes were screened in low B in soil and sand culture, and classified into five groups, namely, very sensitive, sensitive, moderately sensitive, moderately tolerant and tolerant. At very low levels of B, the very sensitive to sensitive genotypes were completely male sterile and set only a few or no grain, while the tolerant genotypes set grain normally. Natural outcrossing was detected in these male sterile plants when a tolerant genotype was growing nearby. Grain set by cross fertilisation was markedly enhanced by a B application directly on the ear of the male sterile plants. Three practical implications are suggested. Firstly, genotypes that are tolerant to low B can provide a solution for grain set failure caused by B deficiency. Secondly, the potential for outcrossing in male sterile B deficient wheat has to be taken into account in the maintenance of pure lines in low B soils even though wheat is normally self pollinated. Thirdly, a simple and novel method for hybridization is suggested, in which B deficiency is used as fertility selective medium and male sterile female parents and fertile male parents are provided by genotypic variation in the response to low B.
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