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  • Development
  • Rheology
  • Springer  (294)
  • American Geophysical Union  (6)
  • American Geophysical Union (AGU)
  • American Physical Society
  • Nature Publishing Group
  • 1995-1999  (161)
  • 1980-1984  (78)
  • 1970-1974  (61)
  • 1935-1939
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  • 1
    Electronic Resource
    Electronic Resource
    Science, technology and society in East Asia: Frameworks for the challenges of the next century (1999)
    Springer
    AI & society 13 (1999), S. 247-262 
    Details
    ISSN: 1435-5655
    Keywords: China ; Development ; East Asia ; Globalisation ; Information and communication technology ; Science and technology policy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Notes: Abstract Science and technology development is increasingly driven by the requirements of a globalising world economy. Information and communication technologies both support this globalisation process, and provide opportunities to participate in it. However, the process of development is not uniform. Within nation states and within regions there is considerable inequity in the scale and pace of development. Successful science and technology policies require an understanding of location within a global production network and of local capabilities. This paper explores the prospects for such policies in the context of North East Asia.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01174780
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  • 2
    Electronic Resource
    Electronic Resource
    A CAD-based tool for development of large layouts (1996)
    Springer
    Research in engineering design 8 (1996), S. 217-228 
    Details
    ISSN: 1435-6066
    Keywords: Complex systems ; Complex train equipment ; 3-D CAD systems ; Design ; Development ; Geometric abstraction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Technology
    Notes: Abstract This paper focuses on the development of a CAD-based tool for the design of complex systems layout, where the primary objective is to compress time to market. We describe the concept for a library which contains necessary information for the conceptual stage of the design process based on the features technology. The stored information has both geometric and non-geometric characteristics. Geometric information is the core of the CAD-based tool. It is in the form of a geometry library which contains systematically abstracted 3-D CAD models. We introduce guidelines for geometric abstraction so that large layouts can be constructed and analysed in 3-D CAD systems. Besides a simplified version of the virtual model of a product, non-geometric information such as functionality, mode of action, costs, material properties, etc., are provided. The method described has been applied for the design of complex train equipment. The results reveal that the lead time required to develop an equipment layout is reduced to about 70 compared with early designs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01597228
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  • 3
    Electronic Resource
    Electronic Resource
    Phase separation effects in the rheology of aqueous solutions of hydroxypropylcellulose (1995)
    Springer
    Rheologica acta 34 (1995), S. 137-146 
    Details
    ISSN: 1435-1528
    Keywords: Rheology ; hydroxypropylcellulose ; liquid crystalline polymers ; phase separation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: Abstract Aqueous solutions of hydroxypropylcellulose (HPC) have been widely used as a model system to study liquid crystalline behavior in polymers. The HPC limiting concentration for mesophase formation in water is about 40% wt, quite independent of molecular weight. Most rheo-optical investigations have been carried out in the concentration range from 50% to 65% wt, on the assumption that only the liquid crystalline phase was present. In this study, by using video-enhanced contrast light microscopy, we show that an isotropic phase in form of tiny droplets is also present at concentrations up to 60% wt, both in quiescent and in sheared samples at room temperature. The isotropic phase can be made to disappear by lowering the temperature. The effects of phase separation on the rheology of the HPC/water system are studied by measuring viscosity as a function of temperature and concentration. A slope of Region I of the viscosity curve close to −0.5 is found only at low temperatures, when the sample is fully anisotropic, whereas an anomalous dependence of the viscosity on temperature is observed when phase separation is significant. This study shows that special care is needed when interpreting experimental results from the HPC/water system in terms of theories for liquid crystalline polymers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00398433
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  • 4
    Electronic Resource
    Electronic Resource
    Rotation of liquid crystalline macromolecules in shear flow and shear-induced periodic orientation patterns (1996)
    Springer
    Rheologica acta 35 (1996), S. 160-167 
    Details
    ISSN: 1435-1528
    Keywords: Rheology ; liquid crystal ; monodomain ; instability ; conoscopy ; shear
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: Abstract We have measured the shear-induced rotation of the nematic director in a liquid crystalline polymer using poly benzyl glutamate (PBG) as model system. PBG is a well characterized synthetic poly (α amino acid) with rigid chain architecture and well defined conformations. For the experiments it is important to start out with a sample in which the molecules are highly aligned with a uniform director. This so-called monodomain morphology is obtained by use of strong magnetic fields and surface modifications of the sample holders. When shearing the monodomain at a constant rate, the macromolecules rotate initially homogeneously until a periodic director pattern develops. These spatially periodic structures emerge in a narrow range of shear strain and, as shearing continues, disintegrate into a chaotic texture. By varying the initial monodomain director with respect to the flow direction (but within the shear planes) we could show that the periodic patterns do not depend on the shear direction; they are governed by the director of the initial monodomain. We observe conoscopically that at high shear rates the texture becomes uniformly aligned. The molecules are aligned preferentially with an angle of about 4° to the shear direction (against vorticity direction). Interestingly, this agrees very well with predictions made by Larson (1990).
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00396043
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  • 5
    Electronic Resource
    Electronic Resource
    Gender, irrigation, and environment: Arguing for agency (1998)
    Springer
    Agriculture and human values 15 (1998), S. 313-324 
    Details
    ISSN: 1572-8366
    Keywords: Development ; Embodiment ; Gender ; Irrigation ; Post-structuralist
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This paper is not a critique of waterpolicies, or an advocacy of alternatives, but rathersuggests a shift of emphasis in the ways in whichgender analysis is applied to water, development, andenvironmental issues. It argues that feministpolitical ecology provides a generally strongerframework for understanding these issues thanecofeminism, but cautions against a reversion tomaterialist approaches in reactions to ecofeminismthat, like ecofeminism, can be static and ignore theagency of women and men. The paper draws attention tothe subjectivities of women and their embodiedlivelihoods as a more useful approach to understandingthe ways in which women relate to water in bothirrigated agriculture and domestic provisioning.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007528817346
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  • 6
    Electronic Resource
    Electronic Resource
    Understanding Conflicts between People and Parks at Ranomafana, Madagascar (1999)
    Springer
    Agriculture and human values 16 (1999), S. 65-74 
    Details
    ISSN: 1572-8366
    Keywords: Conflict management ; Conservation ; Development ; Parks ; Protected areas ; Resident peoples ; Slash-and-burn agriculture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The national park model originating in the unique circumstances of mid-19th century North America has been widely applied in the developing countries of the late 20th century, provoking numerous land-use conflicts between parks and resident peoples. Key factors in understanding these conflicts are examined using the field experience of the Ranomafana National Park in Madagascar. A conflict management strategy is suggested for alleviating such antagonism and facilitating the investigation of mutually acceptable conservation and development pathways.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007572011454
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  • 7
    Electronic Resource
    Electronic Resource
    Suitability of selected leguminous plants for development of Maruca testulalis larvae (1983)
    Springer
    Entomologia experimentalis et applicata 34 (1983), S. 174-178 
    Details
    ISSN: 1570-7458
    Keywords: Lepidoptera ; Pyralidae ; Maruca testulalis ; Pod borer ; Development ; Nutritional suitability ; Host plant ; Crotalaria ; Vigna unguiculata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Les possibilités de développement larvaire de Maruca testulalis sur les fleurs de huit espèces végétales ont été examinées en laboratoire. La comparaison a porté sur Crotalaria retusa, C. juncea, C. saltiana, C. misereniensis, C. mucronata, C. amazonas, Cajanus cajan et l'hôte principal de cette chenille, Vigna unguiculata. En tenant compte de la mortalité larvaire et des indices de croissance (G.I.), ces plantes ont été divisées en trois catégories principales: 1 celles provoquant une mortalité larvaire de 0–30% et ayant des G.I. ≥60%, constituent les plantes hôtes convenables (Vigna unguiculata seule); 2 les plantes qui provoquent une mortalité larvaire de 30≤50% et ont des G.I. de 30 à 60% de la plante hôte principale (Cajanus cajan, Crotalaria amazonas, C. saltiana, C. mucronata; 3 les plantes qui causent 50–100% de mortalité larvaire et dont les G.I. sont inférieurs à 30% de la plante hôte principale (Crotalaria retusa, C. juncea, C. misereniensis). Les résultats déjà publiés sur les choix du lieu de ponte des femelles et l'utilisation de C. juncea comme plante piège, sont discutés à la lumière de ces données nouvelles.
    Notes: Abstract Flowers of eight plant species were evaluated under laboratory conditions for their suitability as larval growth media for the cowpea pod borer, Maruca testulalis. The plants tested were Crotalaria retusa, C. juncea, C. saltiana, C. misereniensis, C. amazonas, Cajanus cajan and the principal host of the borer, Vigna unguiculata (cowpea), was included for comparison. Based on the data obtained on larval mortality and growth indices (GI) the plants were divided into 3 categories namely: I: Those causing 0–30% mortality and having GI value ≥60% form suitable host plants. This group only included V. unguiculata. — II: Those plant species causing 30≤50% larval mortality and having GI value 30%≤60% of the principal host plant (Cajanus cajan, Crotalaria amazonas, C. saltiana, C. mucronata). This group of species is marginally suitable as hosts. — III: Plants causing 50–100% larval mortality and having GI value ≤30% of principal host plant (C. retusa, C. juncea and C. misereniensis). Previously published data on the oviposition preference of the adult moth are discussed in the light of the present findings and the use of C. juncea as a possible trap crop.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00338666
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  • 8
    Electronic Resource
    Electronic Resource
    Cyromazine-sensitive stages of housefly development: Influence of penetration, metabolism and persistence on potency (1983)
    Springer
    Entomologia experimentalis et applicata 34 (1983), S. 251-256 
    Details
    ISSN: 1570-7458
    Keywords: Cyromazine ; Musca domestica ; Housefly ; Development ; Sensitivity ; Penetration ; Insect growth regulator
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé La cyromazine (N-cyclopropyl-1,3,5-triazine-2,4,6-triamine or CGA-72662) est un puissant inhibiteur de développement chez Musca domestica Le traitement des adultes par voie orale n'altère pas l'éclosion des œufs, mais inhibe fortement le développement des larves. Incorporée au milieu larvaire, elle donne une DL50 de 0.11 ppm. La sensibilité des pupes, traitées par application topique, décroit rapidement avec l'âge et est dépendante du solvant utilisé. Une heure après pupaison et avec le tétrahydrofuran comme vecteur, la DL50 est de 3 μg/g, soit 0.2 μg/g pour la pupe sans puparium. Comparé au méthanol. le tétrahydrofuran facilite la pénétration de la cyromazine pendant ce stade critique. La pénétration est également un facteur de la résistance des pupes agées. La toxicité de la cyromazine apparait rait donc principalement dépendante de sa vitesse de pénétration et de son accumulation dans l'organisme plutôt que de son métabolisme. Juste après pupaison, la jeune pupe chez M. domestica s'avère être un matériel sensible et pratique pour l'étude du mode d'action de la cyromazine.
    Notes: Abstract Cyromazine is a potent inhibitor of housefly (Musca domestica L.) larval development when administered orally to adults or incorporated into larval media, and of pupal metamorphosis on topical application shortly before or after pupariation. Pupae are most sensitive to cyromazine within 1 hr after pupariation and with tetrahydrofuran as the carrier solvent, giving topical LD50 values of 3 μg/g for the total puparium plus pupa and 0.2 μg/g calculated for the pupa only. Tetrahydrofuran is more effective than methanol in facilitating rapid cyromazine penetration during this critical pupal stage. The rate of cyromazine penetration or accumulation at sensitive stages appears to be more critical than the extent of metabolism in limiting cyromazine toxicity. Houseflies shortly after pupariation provide a sensitive and convenient organism for studies on cyromazine mode of action.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00186921
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  • 9
    Electronic Resource
    Electronic Resource
    Cooling and crystallization of lava in open channels, and the transition of Pāhoehoe Lava to 'A'ā (1999)
    Springer
    Bulletin of volcanology 61 (1999), S. 306-323 
    Details
    ISSN: 1432-0819
    Keywords: Key words Kīlauea ; Basalt crystallization ; pāhoehoe ; 'a'ā ; Rheology ; Lava flow morphology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract  Samples collected from a lava channel active at Kīlauea Volcano during May 1997 are used to constrain rates of lava cooling and crystallization during early stages of flow. Lava erupted at near-liquidus temperatures (∼1150  °C) cooled and crystallized rapidly in upper parts of the channel. Glass geothermometry indicates cooling by 12–14  °C over the first 2 km of transport. At flow velocities of 1–2 m/s, this translates to cooling rates of 22–50  °C/h. Cooling rates this high can be explained by radiative cooling of a well-stirred flow, consistent with observations of non-steady flow in proximal regions of the channel. Crystallization of plagioclase and pyroxene microlites occurred in response to cooling, with crystallization rates of 20–50% per hour. Crystallization proceeded primarily by nucleation of new crystals, and nucleation rates of ∼104/cm3s are similar to those measured in the 1984 open channel flow from Mauna Loa Volcano. There is no evidence for the large nucleation delays commonly assumed for plagioclase crystallization in basaltic melts, possibly a reflection of enhanced nucleation due to stirring of the flow. The transition of the flow surface morphology from pāhoehoe to 'a'ā occurred at a distance of 1.9 km from the vent. At this point, the flow was thermally stratified, with an interior temperature of ∼1137  °C and crystallinity of ∼15%, and a flow surface temperature of ∼1100  °C and crystallinity of ∼45%. 'A'ā formation initiated along channel margins, where crust was continuously disrupted, and involved tearing and clotting of the flow surface. Both observations suggest that the transition involved crossing of a rheological threshold. We suggest this threshold to be the development of a lava yield strength sufficient to prevent viscous flow of lava at the channel margin. We use this concept to propose that 'a'ā formation in open channels requires both sufficiently high strain rates for continued disruption of surface crusts and sufficient groundmass crystallinity to generate a yield strength equivalent to the imposed stress. In Hawai'i, where lava is typically microlite poor on eruption, these combined requirements help to explain two common observations on 'a'ā formation: (a) 'a'ā flow fields are generated when effusion rates are high (thus promoting crustal disruption); and (b) under most eruption conditions, lava issues from the vent as pāhoehoe and changes to 'a'ā only after flowing some distance, thus permitting sufficient crystallization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004450050299
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  • 10
    Electronic Resource
    Electronic Resource
    Osteocyte types in the developing mouse calvarium (1973)
    Springer
    Calcified tissue international 12 (1973), S. 259-279 
    Details
    ISSN: 1432-0827
    Keywords: Osteocyte ; Calvarium ; Histology ; Development ; Calcium ; Phosphorus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé Des cartes tissulaires ainsi que les caractéristiques et propriétés cellularires ont été relevées au cours d'une étude de microscopie optique du développement de la calotte cranienne de souris, avant la naissance, jusqu'au 26ème jour. Les population d'ostéocytes de moitiés droite et gauche de ces calottes sont semblables, mais décroissent avec le temps dans un volume donné. De petites plages limitées de matrice osseuse se colorent pour le phosphate (ou carbonate) de façon plus nette que la matrice environnante, qui se colore légèrement après coupe. Les divers types ostéocytaires se distinguent par les réactions histochimiques du calcium et du phosphate, qui sont associés dans les cellules osseuses de façon complexe, variant dans le temps et la localisation. Ces deux constituants ne sont pas toujours présent dans les cellules des diverses régions, ou dans la même localisation dans un type cellulaire donné. En tenant compte des changements visible dans les divers types cellulaires avec le temps, dans des régions données, une hypothèse de “charge” et “décharge” cellulaire est émise.
    Abstract: Zusammenfassung Anläßlich einer mikroskopischen Studie über die Entwicklung des Mäuse-Calvariums von der Pränatalperiode bis zu 26 Tagen nach der Geburt wurden auch die Gewebetopographie sowie die Charakteristica und Eigenschaften der Zellen aufgezeichnet. Die Osteocyten-populationen in der linken und rechten Hälfte des Calvariums waren sich gleich, nahmen jedoch bei einem gegebenen Volumen mit der Zeit ab. Kleine isolierte Stellen von Knochen-matrix konnten leichter auf Ph sphat (oder Carbonat) angefärbt werden, als die umgebende Matrix, welche sich wohl am Schnitt, nicht aber am ganzen Stück färben ließ. Die verschiedenen Typen von Osteocyten wurden aufgrund histochemischer Calcium- und Phosphat-reaktionen bestimmt. Calcium und Phosphat waren innerhalb der Knochenzellen auf komplexe Art miteinander verbunden, die je nach Zeit und Lagerung unterschiedlich war. Zellen in abgetrennten Bereichen enthielten nicht immer Calcium und Phosphat und beide waren bei einer bestimmten Zellenart auch nicht immer am gleichen Ort abgelagert. Aufgrund der an ausgewählten Stellen beobachteten, zeitlich bedingten Veränderungen innerhalb der verschiedenen Zelltypen wird vorgeschlagen, daß es sich dabei um eine Sequenz von “Ladung” und “Entladung” der Zellen handelt.
    Notes: Abstract Tissue maps, and cell characteristics and properties were recorded in a study under the optical microscope of the development of the mouse calvarium from pre-natal to 26 days. Osteocyte populations in left and right halves of the calvarium were similar, but decreased with time for a given volume. Small isolated areas of bone matrix stained for phosphate (or carbonate) in a more readily available form from that in the surrounding matrix, which could be stained after sectioning but failed to stain in bulk. Osteocyte types were defined on the basis of histochemical methods for calcium and phosphate, which were associated inside bone cells in a complex manner, varying with time and position. The calcium and phosphate were not always present within the cell in discrete regions and were not always present in the same place in a given cell type. On the basis of a study of changes in cell types with time in selected sites a sequence of “loading” and “unloading” is proposed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02013740
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  • 11
    Electronic Resource
    Electronic Resource
    Mucopolysaccharides and collagen in prenatal and postnatal human development (1974)
    Springer
    Calcified tissue international 16 (1974), S. 209-217 
    Details
    ISSN: 1432-0827
    Keywords: Mucopolysaccharides ; Collagen ; Calvarium ; Development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract Mucopolysaccharides (MPS) and collagen have been analyzed in different types of cartilage and some other tissues at various stages of prenatal and postnatal human development. The concentration of the chondroitin sulfate-type MPS increased from the 38th embryonal day, the 6-sulfate exceeding in amount the 4-sulfate. The increase in collagen concentration was accompanied by a decrease in the proportion of neutral salt-soluble collagen. There was no accumulation of chondroitin sulfates in the calvaria. A maximal concentration of MPS of a low molecular weight occurred in the skin and in the ear pinna after the 10th prenatal week. Postnatally, the MPS-concentration in the iliac crest and in the tibial articular cartilages decreased gradually, the highest values being reached in the newborn; the collagen-concentration showed an approximately reciprocal course.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02008228
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  • 12
    Electronic Resource
    Electronic Resource
    The custom fitting problem and the evolution of developmental systems (1974)
    Springer
    Journal of molecular evolution 3 (1974), S. 109-113 
    Details
    ISSN: 1432-1432
    Keywords: “Custom Fitting” ; Development ; Evolution ; Antibody response ; Genetic Redundancy ; Perturbation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary When the complexity of a developmental system evolves to a certain point, appreciable variation must occur in the process. The problem the biologist faces is whether this point constitutes a limit to the evolution of complexity in developmental systems. If not, what mechanisms are employed to cope with the problem ? The problem—essentially one in “custom fitting” of parts, — and the possible solution(s) to it that have evolved are discussed. The antibody producing system appears to be one that “solves” the custom-fitting problem.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01796556
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  • 13
    Electronic Resource
    Electronic Resource
    Ontogeny of the electric organ discharge and the electric organ in the weakly electric pulse fish Brachyhypopomus pinnicaudatus (Hypopomidae, Gymnotiformes) (1997)
    Springer
    Journal of comparative physiology 181 (1997), S. 111-119 
    Details
    ISSN: 1432-1351
    Keywords: Key words Weakly electric fish ; Gymnotiformes ; Development ; Electric organ ; Electric organ discharge
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract I recorded the electric organ discharges (EODs) of 331 immature Brachyhypopomus pinnicaudatus 6–88 mm long. Larvae produced head-positive pulses 1.3 ms long at 7 mm (6 days) and added a second, small head-negative phase at 12 mm. Both phases shortened duration and increased amplitude during growth. Relative to the whole EOD, the negative phase increased duration until 22 mm and amplitude until 37 mm. Fish above 37 mm produced a “symmetric” EOD like that of adult females. I stained cleared fish with Sudan black, or fluorescently labeled serial sections with anti-desmin (electric organ) or anti-myosin (muscle). From day 6 onward, a single electric organ was found at the ventral margin of the hypaxial muscle. Electrocytes were initially cylindrical, overlapping, and stalk-less, but later shortened along the rostrocaudal axis, separated into rows, and formed caudal stalks. This differentiation started in the posterior electric organ in 12-mm fish and was complete in the anterior region of fish with “symmetric” EODs. The lack of a distinct “larval” electric organ in this pulse-type species weakens the hypothesis that all gymnotiforms develop both a temporary (larval) and a permanent (adult) electric organ.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003590050098
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  • 14
    Electronic Resource
    Electronic Resource
    Ontogeny of rhythmic motor networks in the stomatogastric nervous system (1999)
    Springer
    Journal of comparative physiology 185 (1999), S. 361-365 
    Details
    ISSN: 1432-1351
    Keywords: Key words Central pattern generators ; Development ; Homarus gammarus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We used the lobster Homarus gammarus to study the ontogeny of neural networks involved in rhythmic behaviours. Since in the adult the neural networks belonging to the stomatogastric nervous system and controlling the rhythmic movements of the foregut are well characterised, we have studied them during ontogeny. While this foregut develops slowly throughout embryonic and larval stages, the neuronal population of these motor networks is quantitatively established since the mid-embryonic period. Moreover, in the embryo, this neural population is organised into a single functional network that displays a unique motor output. By contrast, in the adult the same neuronal elements are organised into three neural networks that express independent motor programs. Our results indicate that the multiple adult networks are partitioned progressively from a single embryonic network during development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003590050395
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  • 15
    Electronic Resource
    Electronic Resource
    A nose that looks like a hand and acts like an eye: the unusual mechanosensory system of the star-nosed mole (1999)
    Springer
    Journal of comparative physiology 185 (1999), S. 367-372 
    Details
    ISSN: 1432-1351
    Keywords: Key words Cortical magnification ; Somatosensory cortex ; Development ; Evolution ; Behavior
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The star-nosed mole (Condylura cristata) has a snout surrounded by 22 fleshy and mobile appendages. This unusual structure is not an olfactory organ, as might be assumed from its location, nor is it used to manipulate objects as might be guessed from its appearance. Rather, the star is devoted to the sense of touch, and for this purpose the appendages are covered with thousands of small mechanoreceptive Eimer's organs. Recent behavioral studies find that the star acts much like a tactile eye, having a small behavioral focus, or “fovea” at the center – used for detailed explorations of objects of interest. The peripheral and central nervous systems of the mole reflect these behavioral specializations, such that the small behavioral focus on the nose is more densely innervated in the periphery, and has a greatly enlarged representation in the somatosensory cortex. This somatosensory representation of the tactile fovea is not correlated with anatomical parameters (innervation density) as found in other species, but rather is highly correlated with patterns of behavior. The many surprising parallels between the somatosensory system of the mole, and the visual systems of other mammals, suggest a convergent and perhaps common organization for highly developed sensory systems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s003590050396
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  • 16
    Electronic Resource
    Electronic Resource
    Postmetamorphic changes in auditory sensitivity of the bullfrog midbrain (1995)
    Springer
    Journal of comparative physiology 177 (1995), S. 577-590 
    Details
    ISSN: 1432-1351
    Keywords: Metamorphosis ; Anuran ; Audiogram ; Development ; Midbrain
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract During metamorphosis, the lateral line system of ranid frogs (Rana catesbeiana) degenerates and an auditory system sensitive to airborne sounds develops. We examined the onset of function and developmental changes in the central auditory system by recording multi-unit activity from the principal nucleus of the torus semicircularis (TSp) of bullfrogs at different postmetamorphic stages in response to tympanically-presented auditory stimuli. No responses were recorded to stimuli of up to 95 dB SPL from latemetamorphic tadpoles, but auditory responses were recorded within 24 hours of completion of metamorphosis. Audiograms from froglets (SVL 〈 5.5 cm) were relatively flat in shape with high thresholds, and showed a decrease in most sensitive frequency (MSF) from about 2500 Hz to about 1500 Hz throughout the first 7–10 days after completion of metamorphosis. Audiograms from frogs larger than 5.5 cm showed continuous downward shifts in MSF and thresholds, and increases in sharpness around MSF until reaching adult-like values. Spontaneous activity in the TSp increased throughout postmetamorphic development. The torus increased in volume by approximately 50% throughout development and displayed changes in cell density and nuclear organization. These observations suggest that the onset of sensitivity to tympanically presented airborne sounds is limited by peripheral, rather than central, auditory maturation.
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    URL: http://dx.doi.org/10.1007/BF00207187
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    The effect of genotype, age, sex, and caste on response thresholds to sucrose and foraging behavior of honey bees (Apis mellifera L.) (1999)
    Springer
    Journal of comparative physiology 185 (1999), S. 207-213 
    Details
    ISSN: 1432-1351
    Keywords: Key words Honey bee ; Behavior ; Development ; Neurobiology ; Foraging
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Bees derived from artificially selected high- and low-pollen-hoarding strains were tested for their proboscis extension reflex response to water and varying sucrose concentrations. High-strain bees had a lower response threshold to sucrose than low-strain bees among pre-foragers, foragers, queens and drones. Pre-foraging low-strain workers showed ontogenetic changes in their response threshold to sucrose which was inversely related to age. High-strain foragers were more likely to return with loads of water compared to low-strain foragers. Whereas low-strain foragers were more likely to return with loads of nectar. Low-strain nectar foragers collected nectar with significantly higher sucrose concentrations than did the high-strain nectar foragers. Alternatively, low-strain foragers were more likely to return empty compared to high-strain foragers. These studies demonstrate how a genotypically varied sensory-physiological process, the perception of sucrose, are associated with a division of labor for foraging.
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    URL: http://dx.doi.org/10.1007/s003590050379
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    Proprioceptive feedback in locust kicking and jumping during maturation (1996)
    Springer
    Journal of comparative physiology 179 (1996), S. 195-205 
    Details
    ISSN: 1432-1351
    Keywords: Sensory ; Motor pattern ; Development ; Grasshopper ; Campaniform sensilla
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Campaniform sensilla monitor the forces generated by the leg muscles during the co-contraction phase of locust (Schistocerca gregaria) kicking and jumping and re-excite the fast extensor (FETi) and flexor tibiae motor neurones, which innervate the leg muscles. Sensory signals from a campaniform sensillum on the proximal tibia were compared in newly moulted locusts, which do not kick and jump, and mature locusts which readily kick and jump. The activity pattern of FETi during co-contraction was mimicked by stimulating the extensor tibiae muscle. Less force was generated and the spike frequency of the sensory neurone from the sensillum was significantly lower in newly moulted compared to mature locusts. Depolarisation of both FETi and flexor motor neurones as a result of sensory feedback was consequently less in newly moulted than in mature locusts. The difference in the depolarisation was greater than the decrease in the afferent spike frequency suggesting that the central connections of the afferents are modulated. The depolarisation could generate spikes in FETi and maintain flexor spikes in mature but not in newly moulted locusts. This indicates that feedback from the anterior campaniform sensillum comprises a significant component of the drive to both FETi and flexor activity during co-contraction in mature animals and that the changes in this feedback contribute to the developmental change in behaviour.
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    URL: http://dx.doi.org/10.1007/BF00222786
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    Excitatory interactions between antagonistic motor neurones underlying locust kicking and jumping during maturation after the adult moult (1997)
    Springer
    Journal of comparative physiology 181 (1997), S. 231-237 
    Details
    ISSN: 1432-1351
    Keywords: Key words Motor pattern ; Motor neurone ; Insect ; Grasshopper ; Development ; Schistocerca gregaria
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract There is a change in the synaptic connections between motor neurones that underlie locust kicking and jumping during maturation following the adult moult. The fast extensor tibiae (FETi) motor neurone makes monosynaptic excitatory connections with flexor tibiae motor neurones that have previously been implicated in maintaining flexor activity during the co-contraction phase of jumping, in which energy generated by the muscles of a hind leg is stored. The amplitude of the FETi spike decreases when repetitively activated, and this decrement is larger in locusts immediately following the adult moult than in mature locusts. The decrement in␣the FETi spike is correlated with a greater decrease in the amplitude of the flexor excitatory postsynaptic potential (EPSP) in newly moulted locusts and in turn with the failure of these locusts to kick or jump. The results presented here indicate that the developmental change in the connections between the motor neurones contributes to the change in behaviour following the moult.
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    URL: http://dx.doi.org/10.1007/s003590050109
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    Chick eye optics: zero to fourteen days (1996)
    Springer
    Journal of comparative physiology 179 (1996), S. 185-194 
    Details
    ISSN: 1432-1351
    Keywords: Chicks ; Refraction ; Development ; Schematic eye ; Ocular dimensions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Ocular dimensions and refractive state data for chicks 0 to 14 days of age were obtained from 234 untreated control eyes of birds treated unilaterally in previous work involving various defocussing lenses and/or translucent goggles. Refractive state and corneal curvatures were measured in vivo by retinoscopy and ophthalmometry respectively. Intraocular dimensions were measured by A-scan ultrasonography, after which the eyes were removed, weighed and measured. In some cases (n=52) intraocular dimensions and lens curvatures were obtained from frozen sections of enucleated eyes. The hyperopia of hatchling chicks (+6.5+4.0 D) initially decreases rapidly and then more gradually to + 2.0 ± 0.5 D by 16 days. The distribution of refractive errors is very broad at Day 0, but becomes leptokurtotic, with a slight myopic skew, by Day 14. Corneal radius is constant for the first four days, possible as a result of pre-hatching lid pressure, and then increases linearly, as do all lens dimensions, axial diameter and equatorial diameter. Schematic eyes were developed for Days 0, 7, and 14.
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    URL: http://dx.doi.org/10.1007/BF00222785
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    Patterns of muscle activity during different behaviors in chicks: implications for neural control (1996)
    Springer
    Journal of comparative physiology 179 (1996), S. 169-184 
    Details
    ISSN: 1432-1351
    Keywords: Electromyogram ; Kinematics ; Development ; Chicken ; Locomotion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The large behavioral repertoire that spans the embryonic and postembryonic stages of development make chicks an ideal system for identifying patterns of muscle activity that are common to different behaviors and those that are behavior-specific. The main goal of this work was to identify the similar and dissimilar aspects of the recruitment patterns and the regulation of muscle activity during three distinct postembryonic behaviors: walking, swimming and airstepping. We identified two synergies that were common to each of these behaviors. The synergies were not disrupted by the absence of FT1 activity in airstepping. Within each synergy the recruitment time, recruitment order and duration of activity were not rigid, but varied according to the context-specific resistance that the leg encountered. Unlike the other muscles, FT2 activity was not recruited as part of the same synergy in each behavior. When weight-bearing contact with the substrate did not occur, as in swimming and airstepping, as well as in walking in chicks with deafferented legs, FT2 activity was not recruited as part of either synergy, but was recruited during the time between them. Although not identical, embryonic motility and hatching motor pattern both show the two synergies described for the postembryonic behaviors. Like the latter behaviors, the synergies tolerated the absence of activity from specific muscles. Thus, we suggest that the CNS produces different behaviors using many of the same muscles by organizing the patterned activity around two common synergies while permitting the different muscles that participate in a synergy to be modified in tandem or on an individual basis. Furthermore, the common synergies are established early during prenatal development in chicks.
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    A cone-plate apparatus for the in vitro biochemical and molecular analysis of the effect of shear stress on adherent cells (1995)
    Springer
    Methods in cell science 17 (1995), S. 165-176 
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    ISSN: 1573-0603
    Keywords: Biomechanics ; Cell culture ; Endothelium ; Mechanotransduction ; Mechanical stress ; Scientific instruments ; Rheology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Living cells are constantly exposed to a variety of complex mechanical stimuli which are though to be critical in the control of tissue structure and function. Endothelial and smooth muscle cells in the blood vessel are ideal candidates for the study of blood flow-induced cellular regulation. We describe here a cone-plate viscometer apparatus which is specially-designed for studying the effect of fluid shear stress on large populations of adherent cells in vitro. Using conventional polystyrene tissue culture plates, the apparatus is self-contained, fits inside a standard tissue culture incubator, and provides 75–150 cm2 of useful surface area for cell growth. This capability makes it ideal for studying gene regulation using Northern analysis, nuclear runoff transcription, transfection with reporter constructs, as well as immunochemical staining. The closed-volume design of the device is also well-suited for isotopic labelling, pharmacological studies, and for the detection of minute amounts of secreted cell products. The setup allows the use of either steady, time- and direction-varying laminar, or turbulent shear stress. We provide a detailed assembly procedure and review the method for computing shear stress magnitude and Reynolds number. Ink flow analysis, dynamic response characterization, and LDH measurements are presented to confirm the device's fluid mechanical properties and demonstrate the absence of cell injury.
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    URL: http://dx.doi.org/10.1007/BF00996123
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    Effect of azadirachtin on the nutrition, development and biogenic amine levels in the Eastern Death’s Head hawk moth, Acherontia styx (lepidoptera: sphingidae) (1997)
    Springer
    Experimental biology online 2 (1997), S. 1-14 
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    ISSN: 1430-3418
    Keywords: Acherontia styx ; Azadirachtin ; Biogenic amines ; Development ; Nutrition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Previous studies have suggested the involvement of biogenic amines in insect metamorphic events and post-embryonic development. The effect of azadirachtin (AZ), a natural antifeedant and growth-disrupting compound, on the nutrition, development, and biogenic amine contents of the last instar larvae of the Eastern Death’s Head hawk moth, Acherontia styx, was examined. Single doses of AZ, injected into the haemolymph at day 1 post-ecdysis, inhibited food consumption in a dose-dependent manner (ED50 = 0.65±0.08 μg AZ/g body weight), and was found to be highly effective at producing pupal deformities and inhibiting larval growth (0.1–0.2 μg AZ/g body weight range). Biogenic amine contents, namely octopamine (OA), dopamine (DA) and serotonin (5-HT), in the brain and the haemolymph of 4-day- and 8 day-old larvae were analysed using high-performance liquid chromatography (HPLC) with an electrochemical detector (ECD). A dose-response relationship between AZ and biogenic amine contents in the brain and the haemolymph was also established. Low doses of AZ (0.1–0.2 μg AZ/g body weight) caused a dramatic reduction in OA and 5-HT levels in both the brain and the haemolymph. However, higher doses (0.9–1.2 μg AZ/g body weight) were needed to induce a significant reduction in DA levels. The significance of these findings in relation to the possibility of the involvement of biogenic amines in regulating metamorphic events in insects through mediation of juvenile hormone synthesis and/or release is discussed.
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    URL: http://dx.doi.org/10.1007/s00898-997-0015-6
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    The effect of temperature onshibire ts cell clones in the compound eye ofDrosophila melanogaster (1980)
    Springer
    Development genes and evolution 188 (1980), S. 55-63 
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    ISSN: 1432-041X
    Keywords: Drosophila ; Compound eye ; shibire ts ; Development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have analysed the effect of temperature on both developing and adult eye cell clones homozygous forshi ST139, a temperature-sensitive mutant ofDrosophila melanogaster. The mutant gene, autonomous in its cellular expression, causes structural modifications of ommatidial cells when adult clones of cells are exposed to the restrictive temperature (29°C) for several days. However, the mutant phenotype reverses to normal within 4 days at the permissive temperature (20°C). The results of pulse, shift-up and shift-down experiments show that the temperaturesensitive period for developing compound eye cells is from the late second instar up to the early pupa. Cytodifferentiation of compound eye cells is blocked by restrictive temperature treatment during this period, whereas cell proliferation does not seem to be directly affected. These results are discussed with regard to the other known aspects of the phenotype observed in mutant individuals.
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    Developmental profiles of gangliosides in mouse and rat cerebral cortex (1982)
    Springer
    Development genes and evolution 191 (1982), S. 281-284 
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    ISSN: 1432-041X
    Keywords: Mammals ; Brain cortex ; Gangliosides ; Glycoproteins ; AChE ; Development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Developmental profiles of 11 gangliosides, concentration of lipid- and glycoprotein-bound sialic acid, and activity of AChE of the rat and mouse cerebral cortex were followed from the 7th day of gestation to the 21st postnatal day. There are three main changes in ganglioside concentration, which are similar in both species. The first occurs from gestation day 10 until birth: parallel to decreased proliferation, cell migration, and neuroblast differentiation, GM3 and GD3 in mouse cortex and GD3 in the rat's decreases in favor of GQ1b, GT1b, and GD1a. The second occurs from birth until the first postnatal week: Parallel to increased growth and arborization of dendrites and axons as well as synaptogenesis in rats and mice, there is a two-fold rise of GD1a, whereas GQ1b and GT1b remain on a nearly constant level. Concomitantly, GM3 and GD3 decreases. The third period of ganglioside changes starts in the second postnatal week, parallel to onset of myelination, and is characterized by an increase of GM1 in parallel with a decrease of the polysialogangliosides GT1b and GQ1b.
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    Sequence complexity of poly(A) RNA fromPhysarum polycephalum (1982)
    Springer
    Development genes and evolution 191 (1982), S. 341-347 
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    ISSN: 1432-041X
    Keywords: Physarum ; Development ; RNA ; Sequence complexity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Poly(A) RNA from S phase, G2 phase and starved macroplasmodia of Physarum contain mRNA sequences which when translated in vitro, yield similar patterns of polypeptides after fluorography. Reassociation of nick-translated DNA (Cot) allows the isolation of highly labeled single copy DNA which, after saturation hybridization with poly(A) RNA, gives values of 23% for growth and 17% for starvation. Homologous cDNA/poly(A) RNA hybridization reactions (Rot) indicate that 22–28% of the genome is transcribed during growth and 12% during starvation and that about half of the cDNA reacts with 0.1% of the genome and could represent 50–80 RNA species, each present in about 1,000 copies per nucleus. Up to 25,000 different RNA species, 1–5 copies each per nucleus, are estimated to be present during growth, and about 15,000 during starvation. Heterologous cDNA/poly(A) RNA hybridization reactions (Rot) indicate that the RNA sequences in S and G2 phase of the cell cycle are similar, with RNA sequences being more abundant in G2 phase. During starvation about 25% of the sequences present during growth cannot be detected and those sequences present during growth have become diluted during starvation.
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    URL: http://dx.doi.org/10.1007/BF00879622
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    Of mites and zen: expression studies in a chelicerate arthropod confirm zen is a divergent Hox gene (1998)
    Springer
    Development genes and evolution 208 (1998), S. 591-594 
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    ISSN: 1432-041X
    Keywords: Key words Arthropod ; Hox ; Evolution ; Development ; Chelicerate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  We have cloned, from an oribatid mite, a gene homologous to the zerknült (zen) genes of insects and the Hox 3 genes of vertebrates. Hox genes specify cell fates in specific regions of the body in all metazoans studied and are expressed in antero-posteriorly restricted regions of the embryo. This is true of the vertebrate Hox 3 but not of the zen genes, the insect homologs, and it has been proposed that the zen genes have lost their Hox-like function in the ancestor of the insects. We studied expression of a mite Hox 3/zen homolog and found that it is expressed in a discrete antero-posterior region of the body with an anterior boundary coinciding with that of the chelicerate homolog of the Drosophila Hox gene, proboscipedia, and propose that its loss of Hox function in insects is due to functional redundancy due to this overlap with another Hox gene.
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    URL: http://dx.doi.org/10.1007/s004270050219
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    Rheological information from geological structures (1995)
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    Pure and applied geophysics 145 (1995), S. 605-620 
    Details
    ISSN: 1420-9136
    Keywords: Rheology ; structures ; strain ; numerical models
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences , Physics
    Notes: Abstract The contrast in rheological properties between layers of different composition or texture, and between stiff inclusions and their matrix, gives rise to perturbations in flow that result in structures. Theory and modeling allow us to understand the conditions necessary for such structures to form and, conversely, we can use the form of the structures to infer possible rheological conditions for the rocks during natural deformation. We review here several structures and their use as indicators of rheological behavior, based on theory, numerical and experimental models, and observations on naturl structures. Theory predicts that a dominant wavelength/thickness (L d /h) exists for both folding and boudinage that depends on the ratio of viscosities of layer to matrix, the homogeneous shortening undergone by the layer, and the exponent,n, in a flow law of power-law type. The measurement of averageL/h, and of the shortening within the layers allows an estimate of the power-law exponent of the stiff layer to be made. Also, numerical modeling shows that fold hinges become sharper asn increases and the limbs become relatively longer and straighter. The dynamic growth of pinch and swell instabilities only overcomes the kinematic decay in nonlinear flow, thus the existence of pinch and swell is by itself evidence of nonlinear behavior. Strain rate and strain, increase more rapidly away from the neutral surface for a layer of power-law rheology (withn〉1) than for a layer of Newtonian rheology. Thus, strain gradient across a fold hinge, at fixed amplitude or limb dip, increases with increasingn. Porphyroclasts in mylonites develop characteristic rims of recrystallized grains that are drawn out into trails of σ or ° shape by the perturbed flow of the, material around the clast. Experimental evidence suggests that σ shapes occur if flow is linear, whereas nonlinear flow may give rise to the δ shape. The inference of rheological behavior from structures is complementary to the determination of rheological properties of rocks in the laboratory. What data there is suggest that constitutive relations for rocks undergoing ductile deformation in which many structures develop are highly nonlinear. There is general qualitative agreement between flow laws inferred on the basis of experimental results and those inferred from observation of structural characteristics.
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    Need for quality management in research and development (1998)
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    Accreditation and quality assurance 3 (1998), S. 403-405 
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    ISSN: 1432-0517
    Keywords: Key words Quality management ; Research ; Development ; Scientific fraud
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract  There is growing interest in setting up a general concept for quality management and quality control in research and development, which in this case means, for example, research in the fields of medicine and social sciences. This article is a strong plea for a quality management system in all fields of research and development and will probably initiate broad discussion on this delicate topic.
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    URL: http://dx.doi.org/10.1007/s007690050273
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    Transplanted wing and leg imaginal discs in Drosophila melanogaster demonstrate interactions between epidermis and myoblasts in muscle formation (1996)
    Springer
    Development genes and evolution 206 (1996), S. 46-53 
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    ISSN: 1432-041X
    Keywords: Key words Myogenesis ; Development ; Epidermis ; Myoblast ; Gene-expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Adult muscle development in Drosophila is intimately associated with the development of the nervous system and epidermis. During metamorphosis, myoblasts from the wing imaginal disc reach target sites on the developing pupal epidermis and begin the formation of multinucleate myofibres of the dorsal thorax. The paths taken by pupal myoblasts could be specified by the nervous system and/or the epidermis. Using genetically marked donor pupal wing and leg discs transplanted onto pupal hosts, we have generated animals that have ectopic wings or legs and have examined the formation of adult muscle types. We show that thoracic myoblasts migrate over both host and donor epidermis when the transplant site on the host is thoracic. However, when the transplant site is on the abdomen, thoracic myoblasts do not migrate over abdominal epidermis. Our results show that the epidermis plays an important role in determining the migration pattern of myoblasts. Since muscles are multinucleate cells that form by the fusion of myoblasts, one way in which their molecular characteristics could be achieved is by some myoblasts acting as ”founders”. These myoblasts could influence the pattern of gene expression of those nuclei that fuse with them. We have examined, again using disc transplant experiments, if myoblasts on discs have the capacity to express fibre-specific genes as distinct from this property being conferred by other extra-discs myoblasts. Our results demonstrate that disc-associated myoblasts can indeed fuse with each other to express fibre-specific genes. We synthesize the results presented here with those from earlier experiments to suggest a mechanism for muscle patterning in the adult thorax.
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    URL: http://dx.doi.org/10.1007/s004270050029
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    Response to insulin and the expression pattern of a gene encoding an insulin receptor homologue suggest a role for an insulin-like molecule in regulating growth and patterning in Hydra (1996)
    Springer
    Development genes and evolution 206 (1996), S. 247-259 
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    ISSN: 1432-041X
    Keywords: Key words Hydra ; Insulin ; Development ; Receptor ; Evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A gene encoding a receptor protein-tyrosine kinase closely related to the vertebrate insulin receptor has been identified in the Cnidarian Hydra vulgaris. The gene is expressed in both epithelial layers of the adult polyp. A particularly high level of expression is seen in the ectoderm of the proximal portions of the tentacles and in a ring of ectodermal cells at the border between the foot basal disk and body column. The expression pattern of the gene in asexual buds is dynamic; expression is high throughout the newly emerging bud but the area of high expression becomes restricted to the apex as the bud lengthens. When the bud begins hypostome and tentacle formation, a high level of expression appears at the bases of the emerging tentacles. Finally, a ring of high expression appears just above the foot of the bud, completing the pattern seen in the adult polyp. The presence of this receptor and its pattern of expression suggested that an endogenous molecule related to insulin plays a role in regulating cell division in the body column and in differentiation of the tentacle and foot cells in Hydra, with the switch between the two being determined by the level of the receptor. Treatment of Hydra polyps with mammalian insulin caused an increase in the number of ectodermal and endodermal cells undergoing DNA synthesis.
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    URL: http://dx.doi.org/10.1007/s004270050050
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    The spalt-related gene of Drosophila melanogaster is a member of an ancient gene family, defined by the adjacent, region-specific homeotic gene spalt (1996)
    Springer
    Development genes and evolution 206 (1996), S. 315-325 
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    ISSN: 1432-041X
    Keywords: Key words Transcription factor ; Zinc finger ; Molecular evolution ; Development ; spalt gene family
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  We report the full coding sequence of a new Drosophila gene, spalt-related, which is homologous and adjacent to the region-specific homeotic gene, spalt. Both genes have three widely spaced sets of C2H2 zinc finger motifs, but spalt-related encodes a fourth pair of C-terminal fingers resembling the Xenopus homologue, Xsal-1. The degrees of sequence divergence among all three members of this family are comparable, suggesting that the Drosophila genes originated from an ancient gene duplication. The spalt-related gene is expressed with quantitative variations from mid-embryogenesis (8–12 h) to the adult stage, but not in ovaries or early embryos. Expression is localized to limited parts of the body, including specific cell populations in the nervous system. In the wing disc, spalt and spalt-related are expressed in indistinguishable domains; in the nervous system and some other organs the expression patterns extensively overlap but are not identical, indicating that the genes have partially diverged in terms of developmental regulation. A characteristic central set of zinc fingers specifically binds to an A/T-rich consensus sequence, defining some DNA binding properties of this ancient family of nuclear factors.
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    URL: http://dx.doi.org/10.1007/s004270050058
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    sox30: a novel zebrafish sox gene expressed in a restricted manner at the midbrain-hindbrain boundary during neurogenesis (1999)
    Springer
    Development genes and evolution 209 (1999), S. 357-362 
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    ISSN: 1432-041X
    Keywords: Key words Sox ; Zebrafish ; Development ; Brain ; Midbrain-hindbrain boundary
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The Sox family of proteins is thought to act to regulate gene expression in a wide variety of developmental processes. Here we describe the cloning of sox30, a novel sox gene from the zebrafish (Danio rerio). In situ hybridization shows that sox30 is expressed in a restricted manner at the boundary between the midbrain and hindbrain during nervous system development. This expression pattern is in direct contrast to that of most other neuronally expressed Sox genes which are expressed throughout the nervous system.
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    URL: http://dx.doi.org/10.1007/s004270050264
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    Morphogenetic guidance cues can interact synergistically and hierarchically in steering nerve cell growth (1997)
    Springer
    Experimental biology online 1 (1997), S. 1-15 
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    ISSN: 1430-3418
    Keywords: Nerve cell ; Microelectronics ; Development ; Regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Nerve cell growth is influenced by guiding properties of its substratum. Microfabricated cell culture substrata were used to determine whether rat dorsal root ganglia (DRG) nerve cells could detect and integrate simultaneous model adhesive and topographic guidance cues. Interference reflection microscopy demonstrated strips of surface contact under the marginal zone of growth cones on planar surfaces which were coincident with actin immunostaining at the periphery of the C-domain. Clusters of focal contacts below the growth cone C-domain delineated the track edges on adhesive gratings. Neurite extension was guided most effectively by adhesive gratings of 25-μm period where highly aligned cells were typically bipolar. Nanometric steps and differences in surface texture between the adhesive tracks was detected using atomic force microscopy (AFM). Neurites did not align to 12- to 100-μm pitch grooves which were less than 1 μm deep. The proportion of aligned neurites increased with groove depth. Maximum neurite alignment was seen when 6-μm-deep, 25-μm-wide grooves contained superimposed parallel adhesive tracks of matched pitch. Neurites aligned preferentially to adhesive tracks superimposed orthogonally over shallow grooves (1 μm deep). Primary neurites aligned increasingly to grooves with orthogonal adhesive tracks as their depth increased. These neurites frequently had highly branched terminal arbours aligned to the orthogonal adhesive tracks. We conclude that morphogenetic guidance cues can interact synergistically and hierarchically to steer nerve cell growth.
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    URL: http://dx.doi.org/10.1007/s00898-996-0002-3
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    Sugar binding properties of the vitellogenic proteins in the Colorado beetle, demonstrated by hemagglutination and precipitation experiments (1982)
    Springer
    Development genes and evolution 191 (1982), S. 159-162 
    Details
    ISSN: 1432-041X
    Keywords: Vitellogenin ; Hemagglutinin properties ; Yolk formation ; Insects ; Development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The sugar binding properties of 2 important vitellogenic proteins in Colorado beetle hemolymph were demonstrated by hemagglutination and precipitation experiments. The agglutination of human red blood cells by the hemolymph of reproducing females was observed up to a hemolymph dilution of 1/256, irrespective of the blood-group. It increased significantly after trypsinization of the crythrocytes. Vitellogenin 1 was identified as the hemagglutinin. Hemagglutination and hemagglutination inhibition tests showed that this protein has a low affinity for hexosamines and a higher affinity for sulfated polysaccharides. Precipitation tests demonstrated that besides vitellogenin, another major yolk protein, chromoprotein 2, reacts with sulfated polysaccharides. The possibility that there is a specific reaction of the vitellogenic proteins with well defined saccharides on the oocyte surface is discussed. This lectintype reaction may explain the selectivity of yolk precursor endocytosis.
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    Inhibition of protein synthesis in a polyribosome-dependent cell-free system by a specific ribonuclease prepared from the follicles of two different stages of development of the silkmothAntherea pernyi (1984)
    Springer
    Development genes and evolution 194 (1984), S. 25-31 
    Details
    ISSN: 1432-041X
    Keywords: Protein synthesis ; Development ; Antherea pernyi ; Follicles ; Specific ribonuclease
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Crude, cell-free protein-synthesizing systems were prepared from follicles of two different stages of development in the ovariole of the silkmothAntherea pernyi. The efficiency of the translation of natural and synthetic mRNAs in these systems was compared with that in a cell-free wheat germ system. A postmitochondrial extract (S-30) from the follicles almost completely inhibited protein synthesis in a polyribosome-dependent, cell-free systems. A specific ribonuclease, obtained from the post mitochondrial extract by ammonium sulphate precipitation, heat denaturation and DEAE-cellulose chromatography, inhibited polyribosome-dependent protein synthesis. The effect of this specific ribonuclease on the structural integrity of radioactive RNAs and ribosomal subunits, which were isolated from Ehrlich ascites tumor cells, was also studied.
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    Tissue-specific expression of an hsc90 gene and nuclear translocation of the HSC90-related protein during amphibian embryogenesis (1997)
    Springer
    Development genes and evolution 206 (1997), S. 397-406 
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    ISSN: 1432-041X
    Keywords: Key words Heat-Shock Proteins 90 ; Molecular chaperones ; Development ; Amphibian
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Expression and distribution of a constitutive member of the 90 kDa heat-shock protein family, named HSC90, was investigated during amphibian embryonic development. By Northern blot analysis, two hsp90 transcripts (2.5 and 3 kb) which displayed differing developmental regulation were detected during embryogenesis. Expression of the larger transcript (3 kb), which encodes an HSC90-related protein, decreased until the gastrula stage. However, zygotic transcription for this hsc90 gene was found to start from the neurula stage, and the corresponding zygotic hsc90 transcript was specifically located by whole mount in situ hybridization in the anterior neural tube of a late neurula embryo. Later, in a tailbud embryo, hsc90 transcripts were detected in the cephalic region, neural tube, eye vesicles, branchial and mandibular arches and somites. Distribution of the HSC90-related protein was also analysed by immunohistochemistry throughout embryogenesis. As expected, the protein was strongly expressed in the cytoplasm, mainly in the periplasmic area of embryonic tissue cells. Interestingly, HSC90 was also transiently detected in the nuclear area, with this nuclear transfer depending on the chromatin condensation state, up to the blastula stage. During the process of gastrulation, nuclear translocation of HSC90 was also observed at the level of the blastopore dorsal lip, exclusively in cells undergoing invagination.
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    Immunocytochemical and electrophoretic studies on the localization of the major haemolymph proteins (ceratitins) inCeratitis capitata during development (1984)
    Springer
    Development genes and evolution 194 (1984), S. 37-43 
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    ISSN: 1432-041X
    Keywords: Major haemolymph proteins ; Development ; Cuticle ; Immunocytochemistry ; Ceratitis capitata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The developmental profile of the major haemolymph proteins (ceratitins) inCeratitis capitata was studied. Ceratitin concentration in the haemolymph decreases dramatically during the last days of pupal life, while the amounts of ceratitins in whole organism extracts remain unchanged. By electrophoretic, immunological and immunofluorescence techniques it was revealed that ceratitins are reabsorbed by the fat body and a fraction of them is deposited in the cuticle. The possible role of ceratitins is discussed.
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    Expression of calmodulin in Drosophila is highly regulated in a stage- and tissue-specific manner (1997)
    Springer
    Development genes and evolution 206 (1997), S. 541-545 
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    ISSN: 1432-041X
    Keywords: Key words Drosophila ; Calmodulin ; Gene expression ; Development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The expression of the Drosophila calmodulin (CAM) gene is surprisingly complex. The nervous system, which shows intense transcription in embryogenesis, contains no detectable transcripts at the end of larval life, but becomes transcriptionally active again at pupariation. The gut shows high levels of expression throughout the life cycle, except during pupal reorganization. In contrast, CAM expression in the thoracic muscles drops significantly on transition from pupal to adult life. In the testis, transcription is strongly up-regulated prior to meiosis. Growing cells show lower transcript levels than most differentiated tissues and in general, cells with intense exocytotic or endocytotic activity show the highest mRNA levels.
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    URL: http://dx.doi.org/10.1007/s004270050085
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    The Suppressor of fused gene, involved in Hedgehog signal transduction in Drosophila, is conserved in mammals (1999)
    Springer
    Development genes and evolution 209 (1999), S. 294-300 
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    ISSN: 1432-041X
    Keywords: Key words Evolution ; Suppressor of fused ; Hedgehog ; Development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The Suppressor of fused [Su(fu)] gene of Drosophila melanogaster encodes a protein containing a PEST sequence [sequence enriched in proline (P), glutamic acid (E), serine (S) and threonine (T)] which acts as an antagonist to the serine-threonine kinase Fused in Hedgehog (Hh) signal transduction during embryogenesis. The Su(fu) gene isolated from a distantly related Drosophila species, D. virilis, shows significantly high homology throughout its protein sequence with its D. melanogaster counterpart. We show that these two Drosophila homologs of Su(fu) are functionally interchangeable in enhancing the fused phenotype. We have also isolated mammalian homologs of Su(fu). The absence of the PEST sequence in the mammalian Su(fu) protein suggests a different regulation for this product between fly and vertebrates. Using the yeast two-hybrid method, we show that the murine Su(fu) protein can interact directly with the Fused and Cubitus interruptus proteins, known partners of Su(fu) in Drosophila. These data are discussed in the light of their evolutionary relationships.
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    Differential stability of Xenopus c-myc RNA during oogenesis in axolotl Involvement of the 3′ untranslated region in vivo (1995)
    Springer
    Development genes and evolution 205 (1995), S. 182-191 
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    ISSN: 1432-041X
    Keywords: C-myc stability ; Development ; Amphibian
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have used the axolotl oocyte (Ambystoma mexicanum Shaw) to study the stability of exogenously injected Xenopus RNAs. Three different cellular developmental stages have been analysed: (1) the growing oocyte (stage III–IV of vitellogenesis), (2) the full-grown oocyte at the end of vitellogenesis (stage VI) and (3) the progesterone-matured stage VI oocyte. Three exogenous RNAs have been synthesized in vitro from a c-myc Xenopus cDNA clone. One transcript is 2.3 kb long (full length), the second is 1.5 kb long, with most of the 3′ untranslated region (3′UTR) removed, and the third corresponds to the 3′UTR (0.8 kb). After injection or coinjection of these exogenous Xenopus RNAs into axolotl oocytes, the stability of the molecules was studied after 5 min, 6 h and 21 h by extraction of total RNA and Northern blot analysis. Results show a difference in Xenopus RNA stability during axolotl oogenesis. In growing oocytes, the three synthetic transcripts are gradually degraded. The absence of the 3′UTR is not therefore sufficient to stabilize the transcript during early oogenesis. No degradation is observed in full-grown oocytes, suggesting the existence of stabilizing factors at the end of oogenesis. When stage VI oocytes are induced to mature by progesterone, only the 2.3 and 1.5 kb Xenopus RNAs disappear. This suggests a role for germinal vesicle breakdown in this degradation process as well as the existence of a factor present in the nucleus and involved in the specific destabilization of these RNAs after oocyte maturation. This degradation might implicate several destabilizing sequences localized in the coding or in the 3′UTR of the c-myc gene. In contrast, the 0.8 kb transcript (3′UTR) is not degraded during this period and remains very stable. Therefore, degradation appears distinct from one transcript to another and from one region to another within the same molecule. During maturation, the behaviour of the 2.3 and 1.5 kb transcripts is different when coinjected with the 3′UTR, suggesting a role in trans of this untranslated molecule in c-myc stability. Our approach allows us to analyse the role of the coding and 3′UTR regions of the c-myc RNA in the control of mRNA degradation in vivo.
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    Ganglioside changes in the chicken optic lobes and cerebrum during embryonic development (1980)
    Springer
    Development genes and evolution 188 (1980), S. 205-213 
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    ISSN: 1432-041X
    Keywords: Gangliosides ; Development ; Brain ; Chicken
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The developmental profiles of 15 different gangliosides of the optic lobes and cerebrum of the chicken were followed from the 6 th day of incubation to hatching and correlated to morphological development. Five of these gangliosides appearing in both structures between the sixth and tenth day, have not been reported previously in higher vertebrates. Three chromatographed on TLC-plates similarly to GT3, GT2, and GT1c gangliosides, which have been demonstrated in fish brain. One fraction moved just below GQ1b and is suggested, to contain GQ1c. These “novel” gangliosides, which are possibly related to a recently proposed separate and probably phylogenetically older biosynthetic pathway, contained up to 20% of total ganglioside sialic acid. The fifth “novel” fraction, containing up to 16% of total ganglioside-sialic acid, moved below the penta-sialoganglioside GP1 and is suggested to contain hexa-sialogangliosides. There were two main changes in ganglioside synthesis, which were identical in both structures. The first occurred from the sixth to the eleventh day, parallel to decreased proliferation, maximal cell migration and neuroblast differentiation, GD3 and GD2 decreased rapidly in favour of GQ1b, GP1, and to the “novel” fractions, described above. The second occurred from the eleventh to the eighteenth day, parallel to increased growth and arborization of dendrites and axons as well as functional establishment of synaptic contacts, there was a sharp rise in the amount of GD1b, GT1b, and GD1a. Concomitantly the “novel” gangliosides decreased. At hatching GD1a was the predominant ganglioside. GM3, GM2, and GM1 were always minor fractions, each accounting for less than 4% of total ganglioside-sialic acid. GM4 was never detected, indicating neglegible myelinisation until hatching.
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    Studies on the regulation of the branched chain amino acyl-tRNA synthetases of the fungusNeurospora crassa (1980)
    Springer
    Development genes and evolution 189 (1980), S. 171-180 
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    ISSN: 1432-041X
    Keywords: Amino acyl-tRNA synthetases ; Development ; Nuclear-mitochondrial interactions ; Neurospora
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The specific activities of the branched chain amino acyl-tRNA synthetases from the cytosolic and mitochondrial fractions ofN. crassa were low in dormant conidia and increased during germination, reaching a maximum 8 h after inoculation. This stage of development is characterised by high rates of many other cellular activities. The increases in activity of synthetases of both cytosol and mitochondria are inhibited by cycloheximide indicating that they are synthesized on cytoplasmic ribosomes. The mitochondrial synthetases show a stimulation of their specific activity when mitochondrial RNA and protein synthesis are inhibited by either ethidium bromide or chloramphenicol suggesting that a mitochondrial translation product regulates the synthesis of the mitochondrial synthetases. The activities of amino acyl-tRNA synthetases are dependent on energy production. When respiration is uncoupled from oxidative phosphorylation, synthetase specific activities decrease although the activities of other mitochondrial enzymes like NADH-dehydrogenase increase. This phenomenon suggests that more than one mechanism regulates the synthesis of mitochondrial proteins which are formed on cytoplasmic ribosomes. The synthesis of branched chain amino acyl-tRNA synthetases ofNeurospora is neither repressed by their cognate amino acids, nor is there inhibition by the precursors of these amino acids, as has been observed in other amino acyl-tRNA synthetases of various organism includingNeurospora.
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    Electron microscopical investigations of the intercellular contacts during the early cleavage stages ofLymnaea stagnalis (Mollusca, Gastropoda) (1981)
    Springer
    Development genes and evolution 190 (1981), S. 215-220 
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    ISSN: 1432-041X
    Keywords: Mollusca ; Cleavage ; Junctions ; Interaction ; Development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In early cleavage stages ofLymnaea stagnalis, three kinds of intercellular junctions could be distinguished up to the sixth cleavage: intermediate, septate and gap junctions. The first two form “junctional belts” located on the cell border at the periphery of the embryo. For the purpose of our study we were most interested in gap junctions as they are alleged to be structures that allow cell-to-cell communication. Gap junctions first appear at the four cell stage. Up to the sixth cleavage no difference in the distribution pattern could be found between and within each of the four quadrants of the embryo. Some of the cell tiers along the animal-vegetal axis lack gap junctions either between the blastomeres within the tier or between the blastomeres from adjacent tiers. All gap junctions observed in freeze fracture replicas show plaques with an irregular IMP pattern. The average IMP diameter measures 12 nm (SD±2 nm). In stages fixed after the fifth cleavage, gap junctions are found between micromeres at the animal pole and the central 3D macromere. This is in agreement with the presumed interaction between these cells at this stage. The possibility of a transition of non-functional into functional gap junctions after the fifth cleavage is discussed.
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    Gap junction formation between normal and reaggregated endoderm cells ofXenopus laevis neurulae (1982)
    Springer
    Development genes and evolution 191 (1982), S. 143-148 
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    ISSN: 1432-041X
    Keywords: Amphibia ; Junctions ; Interaction ; Development ; Freeze fracture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Using freeze-fracture electron microscopy and fluorescent dye injection we have analysed the contacts between cells of the deeper endoderm taken from neurulae ofXenopus laevis. Endodermal cells in situ have large 1.5 μm diameter gap junctions composed of 8 nm P-face particles and corresponding E-face pits. Beside gap junctions, particle aggregates typical of desmosomal plaques are present but there are no tight junctions. The dissociation of endoderm into single cells involves profound structural alterations in the surface membrane including the complete disappearance of junctional structures among them gap junctions. The reaggregation of endoderm cells leads to the restoration of the surface membrane IMP (Intra Membrane Particle) pattern and, after ca. 30 min, to the establishment of functional pathways allowing for the intercellular transfer of fluorescent dye. Concomitantly gap junctions reappear. The observation that the dissociation and reaggregation of endodermal cells involves IMP alterations which go beyond the cell junctions themselves is discussed as an adaptation of the plasma membrane to changing environmental conditions.
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    Dye-coupling between blastomeres in early embryos ofPatella vulgata (mollusca, gastropoda): Its relevance for cell determination (1983)
    Springer
    Development genes and evolution 192 (1983), S. 262-269 
    Details
    ISSN: 1432-041X
    Keywords: Mollusca ; Interaction ; Development ; Dye-coupling ; Gap junctions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary During the early development of the molluscPatella, the dorsoventral axis is established after the fifth cleavage due to direct interaction between the animal micromeres and one of the vegetal macromeres. This vegetal macromere is thereby induced to become the mesentoblast mother cell (3D). In this study we have examined intercellular communication in earlyPatella embryos by monitoring the transfer of the fluorescent dye, Lucifer Yellow, upon iontophoretic injection into blastomeres between the second and sixth cleavage. Up to the fifth cleavage dye transfer is detectable neither inin toto embryos nor in serial sections. Shortly after the fifth cleavage dye-coupling between blastomeres becomes apparent. This occurs approximately 40 min before the interaction between animal micromeres and the future mesentoblast mother cell. Inspection of serially sectioned embryos after dye-iontophoresis in either animal micromeres or in the central macromere 3D showed the absence of direct dye-transfer between these cells at the stage of interaction. The reduced rate of dye-transfer from the 3D macromere to its dorsal neighbour 2d2 suggests a bilateral symmetrical transfer pattern, the axis of which corresponds with the dorsoventral axis at the sixth cleavage. Cell deletion experiments demonstrated that the establishment of dye-coupling between the vegetal macromeres occurs independently of the interaction between animal and vegetal blastomeres.
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    Major haemolymph proteins inCeratitis capitata: Biosynthesis and secretion during development (1981)
    Springer
    Development genes and evolution 190 (1981), S. 33-39 
    Details
    ISSN: 1432-041X
    Keywords: Ceratitis capitata ; Major haemolymph proteins ; Development ; Fat body ; Secretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The accumulation of major haemolymph proteins (a group of proteins immunologically related to Calliphorin) their biosynthesis in vivo and in organ culture as well as their secretion, has been studied during the late larval stages and white pupae of the Mediterranean fruit flyCeratitis capitata. The accumulation of major haemolymph proteins in the haemolymph, shows a twenty fold increase from the 4-day old larvae to the white pupae stage, while in the fat body there is only a seven fold increase. It is evident from the in vivo and organ culture studies, that the major haemolymph proteins are synthesized during the late larval stage and their synthesis declines abruptly during the stage of white pupae. It seems also that each polypeptide has its own characteristic developmental kinetics of synthesis. The major haemolymph proteins are synthesized in the fat body and are very quickly secreted into the haemolymph.
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    The Drosophila melanogaster homologue of the hsp60 gene is encoded by the essential locus l(1)10Ac and is differentially expressed during fly development (1997)
    Springer
    Development genes and evolution 207 (1997), S. 253-263 
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    ISSN: 1432-041X
    Keywords: Key words Drosophila ; l(1)10Ac locus ; Development ; hsp60 gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The hsp60 (heat-shock protein 60) gene family of molecular chaperones has been a subject of study in numerous systems due to its important role in the correct folding of non-native proteins in development as well as after heat-shock treatment. Here we present the characterization of the first Drosophila hsp60 homologue. Drosophila HSP60 is most closely related (72% identity across the entire protein sequence) to the mouse mitochondrial HSP60. Western blot experiments indicate that Drosophila HSP60 is enriched in the mitochondrial fraction. The distribution of HSP60 protein is dynamic during fly embryogenesis, suggesting that various cell types might have different HSP60 requirements. The molecular analysis of a P-element-induced mutation that affects the l(1)10Ac locus shows that the transposon is inserted in a 3-kb intron present in the hsp60 gene. By genetic rescue experiments we prove that Drosophila HSP60 is encoded by the essential locus l(1)10Ac opening the possibility for detailed genetic analysis of HSP60 functions in the fly.
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    Lectin binding sites during Drosophila embryogenesis (1995)
    Springer
    Development genes and evolution 204 (1995), S. 229-243 
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    ISSN: 1432-041X
    Keywords: Glycan structures ; Lectins ; Drosophila Embryogenesis ; Development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The spectrum of lectin binding sites as it emerges during embryonic development of Drosophila was analysed by means of fluorescein-labelled lectins. As development and morphogenesis proceed, the reaction pattern becomes more and more complex. Mannose/glucose-, mannose-, N-acetylglucosamine- and poly-N-ace-tylglucosamine-specific lectins bind ubiquitously. Nuclear envelopes only have binding sites for wheat germ agglutinin. N-acetylgalactosamine-binding lectins are specific for ectodermal derivatives. Gaβ-3-N-acetylgalac-tosamine-binding lectins are highly selective markers for neural structures, haemocytes and Garland cells. It is also shown that Drosophila laminin is differentially glycosylated. The possible implications of differential and germ layer-specific glycosylation are discussed.
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    Developmental regulation of presence of binding sites for neoglycoproteins and endogenous lectins in various embryonic stages of human lung, liver and heart (1995)
    Springer
    Development genes and evolution 204 (1995), S. 344-349 
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    ISSN: 1432-041X
    Keywords: Development ; Lectin ; Neoglycoprotein Glycoconjugate ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Protein-carbohydrate interactions are supposed to play key roles in the mechanisms of cell adhesion, biosignalling and intracellular routing, warranting the analysis of the developmental course of expression of epitopes of this system. Thus, a panel of carrier-immobilized carbohydrate ligands was used as probes, namely lactose,N-acetylgalactosamine,N-acetylglucosamine, mannose, fucose and maltose. Additionally, an antibody to an endogenous β-galactoside-binding lectin (anti-galectin-1), the biotinylated lectin and two further human lectins, namely the macrophage migration inhibitory factor-binding sarcolectin and serum amyloid P component (SAP) that displays selectivity for sulphated sugars and mannose-6-phosphate, were included. They enabled us to assess the extent of the presence of respective binding sites in fixed sections from human lungs (pulmonary epithelial cells), livers (hepatocytes) and hearts (myocard cells) of 10–50 weeks gestation. Invariably, specific binding was detected in the three organ types, at least in certain stages. In most of the cases, the intensity of staining exhibited developmental regulation. The apparent patterns reveal similarities between the different cell types, as seen with immobilizedN-acetylglucosamine as well as with labelled galectin-1 and sarcolectin. However, drastic differences among such patterns with nearly opposite developmental courses do also occur, as detected for carrier-attached mannose and maltose residues. These results point to a potential importance for the detected glycohistochemical features in human development and substantiate the possibility of differential regulation of the presence of binding sites for distinct sugars within a certain organ and between the individual cell types of the monitored organs.
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    Maternal factors and the evolution of developmental mode: Evolution of oogenesis in Heliocidaris erythrogramma (1999)
    Springer
    Development genes and evolution 209 (1999), S. 275-283 
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    ISSN: 1432-041X
    Keywords: Key words Echinoid ; Oogenesis ; Development ; Evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Evolutionary change in developmental mode in sea urchins is closely tied to an increase in maternal provisioning. We examined the oogenic modifications involved in production of a large egg by comparison of oogenesis in congeneric sea urchins with markedly different sized oocytes and divergent modes of development. Heliocidaris tuberculata has small eggs (95 µm diameter) and the ancestral mode of development through feeding larvae, whereas H. erythrogramma has large eggs (430 µm diameter) and highly modified non-feeding lecithotrophic larvae. Production of a large egg in H. erythrogramma involved both conserved and divergent mechanisms. The pattern and level of vitellogenin gene expression is similar in the two species. Vitellogenin processing is also similar with the gonads of both species incorporating yolk protein from coelomic and hemal stores into nutritive cells with subsequent transfer of this protein into yolk granules in the developing vitellogenic oocyte. Immunocytology of the eggs of both Heliocidaris species indicates they incorporate similar levels of yolk protein. However, H. erythrogramma has evolved a highly divergent second phase of oogenesis characterised by massive deposition of non-vitellogenic material including additional maternal protein and lipid. Maternal provisioning in H. erythrogramma exhibits recapitulation of the ancestral vitellogenic program followed by a novel oogenic phase with hypertrophy of the lipogenic program being a major contributor to the increase in egg size.
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    Homeotic gene expression in the wild-type and a homeotic mutant of the moth Manduca sexta (1999)
    Springer
    Development genes and evolution 209 (1999), S. 460-472 
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    ISSN: 1432-041X
    Keywords: Key words Insect ; Development ; Homeotic mutant ; Appendage ; Spiracle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Antibodies were used to examine the expression patterns of Antennapedia (Antp), Ultrabithorax (Ubx), Ubx and abdominal-A combined(Ubx/abd-A),and Distalless (Dll) in the embryos of the moth Manduca sexta. We found that the spatial and temporal pattern of Antp expression in Manduca was correlated with the anterior migration of two patches of epithelium that include the anterior-most tracheal pits, and with the development of functional spiracles. Ubx expression showed an intricate pattern which suggests complex regulation during development. Throughout Manduca embryogenesis the expression of Ubx/Abd-A and Dll was similar to that reported for other insects. However, there was no apparent reduction in Ubx/Abd-A expression in the Manduca abdominal proleg primordia that expressed Dll. The expression of these four proteins was also examined in embryosof the Manduca homozygous homeotic mutant Octopod (Octo). The Octo mutation results in the transformation of A1 and A2 in the anterior direction, with homeotic legs appearing on A1 and occasionally A2. Our results suggest that in Octo animals there is a reduction in the level of Ubx protein expression throughout its domain. Based on homeotic gene expression in wild-type and mutant Manduca and in other insects, we discuss potential roles of homeotic genes in insect morphological evolution.
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    Mouse Dach, a homologue of Drosophila dachshund, is expressed in the developing retina, brain and limbs (1999)
    Springer
    Development genes and evolution 209 (1999), S. 526-536 
    Details
    ISSN: 1432-041X
    Keywords: Key words dachshund ; Retina ; Development ; Limb ; Brain
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The Drosophila genes eyeless, eyes absent, sine oculis, and dachshund cooperate as key regulators of retinal cell-fate determination. Homologues of eyeless (Pax6), eyes absent (Eya1-2), and sine oculis (Six3) have been identified and are expressed in the developing vertebrate eye. We have cloned and characterized the structure and expression of mouse Dach, a homologue of Drosophila dachshund. Sequence analysis reveals the presence of two motifs, DD1 and DD2, which may be involved in the function of Dach/Dachshund as gene regulatory factors. In addition, DD1 shares sequence similarity to N-terminal sequences of Ski and SnoN, which are involved in cellular transformation and differentiation. Mouse and human Dach/DACH were localized to chromosome 14E1 and 13q21.3–22, respectively, by fluorescence in situ hybridization. Finally, in situ hybridization analysis demonstrated that Dach is expressed in similar tissues to those observed in Drosophila, including the embryonic nervous system, sensory organs, and limbs. The finding of Dach expression in the eye completes the list of vertebrate homologues of eyeless, eyes absent, sine oculis, and dachshund which as a group may function to control cell-fate determination in the vertebrate eye.
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    URL: http://dx.doi.org/10.1007/s004270050285
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    The development of cnidarian stinging cells: maturation and migration of stenoteles of Hydra vulgaris (1995)
    Springer
    Development genes and evolution 205 (1995), S. 171-181 
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    ISSN: 1432-041X
    Keywords: Nematocytes ; Development ; Migration ; poly(γ-glutamic acid) ; Hydra
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The late stages of stenotele development and the migration and installation of freshly matured stenoteles in Hydra have been studied by kinetic and immunofluorescence investigations with rhodamine-labelled polyps. It was found that the high concentration of osmotic pressure-generating poly(γ-glutamic acid)s is synthesised exclusively within the lumen of the immature nematocyst. Assembly of the polymers, which is completed after approximately 0.5 days, is accompanied by a swelling of the capsule and ends when the cyst is mature. Active migration of the stenoteles to the tentacles begins only about 1.0 day later, and the time required for installation of a stenotele on the outer surface of the tissue amounts to about another 1.5 days. Furthermore, the results obtained suggest that the disintegration of the clusters of growing stenoteles, which begins 0.5 to 1.0 days before maturation, is a passive process; the ability of a nematocyte to migrate actively to the tentacles is acquired after maturation and might be controlled directly by regulating factors contained in the tissue.
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    Subaqueous, basaltic lava dome and carapace breccia on King George Island, South Shetland Islands, Antarctica (1998)
    Springer
    Bulletin of volcanology 59 (1998), S. 245-261 
    Details
    ISSN: 1432-0819
    Keywords: Key words Lava dome ; Endogenous ; Basaltic ; Submarine ; Rheology ; Antarctica
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geosciences
    Notes: Abstract  On King George Island during latest Oligocene/earliest Miocene time, submarine eruptions resulted in the emplacement of a small (ca. 500 m estimated original diameter) basalt lava dome at Low Head. The dome contains a central mass of columnar rock enveloped by fractured basalt and basalt breccia. The breccia is crystalline and is a joint-block deposit (lithic orthobreccia) interpreted as an unusually thick dome carapace breccia cogenetic with the columnar rock. It was formed in situ by a combination of intense dilation, fracturing and shattering caused by natural hydrofracturing during initial dome effusion and subsequent endogenous emplacement of further basalt melt, now preserved as the columnar rock. Muddy matrix with dispersed hyaloclastite and microfossils fills fractures and diffuse patches in part of the fractured basalt and breccia lithofacies. The sparse glass-rich clasts formed by cooling-contraction granulation during interaction between chilled basalt crust and surrounding water. Together with muddy sediment, they were injected into the dome by hydrofracturing, local steam fluidisation and likely explosive bulk interaction. The basalt lava was highly crystallised and degassed prior to extrusion. Together with a low effusion temperature and rapid convective heat loss in a submarine setting, these properties significantly affected the magma rheology (increased the viscosity and shear strength) and influenced the final dome-like form of the extrusion. Conversely, high heat retention was favoured by the degassed state of the magma (minimal undercooling), a thick breccia carapace and viscous shear heating, which helped to sustain magmatic (eruption) temperatures and enhanced the mobility of the flow.
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    Naphthylamidase* in ameloblasts during enamel maturation (1971)
    Springer
    Calcified tissue international 7 (1971), S. 267-276 
    Details
    ISSN: 1432-0827
    Keywords: Tech ; Development ; Enamel ; Enzyme ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé L'activité en naphtylamidase est étudiéc au niveau des incisives et molaires de rat, à divers stades de développement. Du L-leucyl-4-methoxy-2-naphtylamide, du L-alanyl-4-methoxy-2-naphtylamide, du L-leucyl-2-naphthylamide et du DL-alanyl-2-naphtylamide sont utilisés comme substrats: du bleu rapide B et du grenat rapide GBC sont employés comme sels de diazonium. Le naphtylamidase n'est pas visible au niveau de dents, en voie de dévelopment, au cours de la formation matricielle de l'émail. A la fin de ce stade, le naphtylamidase est présent au niveau de l'extrémité distale des améloblastes, près de la surface de l'émail. L'activité enzymatique reste identique jusqu'au moment de la fusion de l'épithélium dentaire et de l'épithélium buccal, au moment de l'éruption de la dent dans la cavité buccale. On ne rencontre pas de naphtylamidase au niveau d'autres tissues dentaires; cependant une activité marquée est observée dans les ostéoclastes au niveau des surfaces de résorption de l'os alvéolaire, entourant les dents, en voie de développement et d'éruption, et dans certaines régions du tissu conjonctif.
    Abstract: Zusammenfassung Die Aktivität der Naphthylamidase wurde in den Backen- und Schneidezähnen von Ratten in verschiedenen Entwicklungsstufen studiert. Als Substrate wurden L-leucyl-4-methoxy-2-naphthylamid, L-alanyl-4-methoxy-2-naphthylamid, L-leucyl-2-naphthylamid und DL-alanyl-2-naphthylamid verwendet; als Diazoniumsalze dienten Echtblau B und Echt-Granat GBC. Naphthylamidase konnte während der Schmelzmatrixbildung im Zahn nicht nachgewiesen werden. Nach Abschluß dieser Phase erschien Naphthylamidase in den distalen Enden der Ameloblasten, nahe bei der Schmelzoberfläche. Die Enzymtätigkeit blieb am selben Ort lokalisiert, bis das Zahnepithel, im Augenblick wo der Zahn in die Mundhöhle durchstößt, in das Mundepithel überging. Naphthylamidase wurde in anderen Zahngeweben nicht gefunden, aber eine deutliche Aktivität konnte in gewissen Bezirken des Bindegewebes sowie in den Osteoklasten der resorbierenden Oberflächen vom alveolären Knochen festgestellt werden, welcher die sich bildenden und die hervorstoßenden Zähne umgibt.
    Notes: Abstract Naphthylamidase activity was studied in rat molar and incisor teeth at different stages of development. L-leucyl-4-methoxy-2-naphthylamide, L-alanyl-4-methoxy-2-naphthylamide, L-leucyl-2-naphthylamide and DL-alanyl-2-naphthylamide were used as substrates and Fast blue B and Fast Garnet GBC as diazonium salts. Naphthylamidase was not demonstrable in the teeth during enamel matrix formation. After the termination of this stage, naphthylamidase was present in the ameloblasts in their distal ends close to the enamel surface. The enzyme activity retained this localization until the dental epithelium fused with the oral epithelium at the time of tooth eruption into the oral cavity. Naphthylamidase was not found in other dental tissues, but marked activity was found in osteoclasts at the resorbing surfaces of alveolar bone surrounding the developing and erupting teeth and in certain areas of the connective tissue.
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    Tetracycline effects on statolith and nematocyst differentiation inAurelia (1972)
    Springer
    Calcified tissue international 9 (1972), S. 122-130 
    Details
    ISSN: 1432-0827
    Keywords: Tetracycline ; Development ; Calcification ; Statolith ; Nematocysts ; Aurelia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé L'effet de la tétracycline HCl sur la synthèse de statolithes de sulfate de calcium chezAurelia a été étudié. La tétracycline inhibe la synthèse de statolithes et nématocystes à un stade précoce de strobilation. La tétracycline, cependant, n'est pas incorporée dans les statolithes ou nématocystes en formation. Comme la tétracycline ne se combine pas avec le calcium des statolithes de sulfate de calcium dihydraté d'Aurelia, l'explication des effets d'inhibition sur la différenciation de statolithes et nématocystes ne semble pas liée avec un facteur en rapport avec l'incorporation. Des étudesin vitro de quatre systèmes inorganiques de calcium et de tétracycline montrent que le sulfate de calcium dihydraté (gypse) n'incorpore pas la tétracycline: il en est de même de son équivalent isostructural, le phosphate de calcium hydrogéné dihydraté (brushite). Le carbonate de calcium et le phosphate de calcium (apatite) incorpore la tétracycline. L'explication des différences de comportement du calcium peut être liée à la structure cristalline des composés respectifs, et, en particulier, au fait que l'ion Ca est prêt ou non à réagir avec la tétracycline.
    Abstract: Zusammenfassung Es wird über die Wirkung von Tetracyclinchlorhydrat auf die Synthese von Calciumsulfat-Statolithen beiAurelia berichtet. Wird das Tetracyclin in einem Frühstadium der Strobilation verabreicht, so hemmt es die Synthese der Statolithen und der Nematocysten. Das Tetracyclin wird jedoch nicht in die sich bildenden Statolithen oder Nematocysten eingebaut. Da sich das Tetracyclin nicht mit dem Calcium der Calciumsulfatdihydrat-Statolithen derAurelia verbindet, so kann dessen Hemmwirkung auf die Statolithen und die sich differenzierenden Nematocysten offenbar nicht mit einem einbaubedingten Faktor erklärt werden. Untersuchunge, die in vitro mit vier verschiedenen anorganischen Calciumsalzen und Tetracyclin ausgeführt wurden, zeigten, daß weder Calciumsulfatdihydrat (Gips), noch dessen isotrukturelles Aequivalent Calciumhydrogenphosphatdihydrat (Bruschit) Tetracyclin einbauen. Dagegen inkorporieren Calciumcarbonat und Calciumphosphat (Apatit) das Tetracyclin. Die Erklärung für dieses unterschiedliche Verhalten der Calciumsalze findet sich in der Kristallstruktur der betreffenden Verbindungen, d.h. es hängt davon ab, ob das Calciumion ür die Reaktion mit Tetracyclin leicht verfügbar ist.
    Notes: Abstract The effect of tetracycline HCl on synthesis of calcium sulphate statoliths inAurelia is reported. Tetracycline inhibits synthesis of statoliths and nematocysts when administered at an early stage of strobilation. The tetracycline, however, is not incorporated into the developing statoliths or nematocysts. As the tetracycline does not combine with the calcium of the calcium sulfate dihydrate statoliths ofAurelia, an explanation for its inhibitory effects on statoliths and nematocyst differentiation apparently does not rest with an incorporation-related factor. In vitro studies of four inorganic calcium systems and tetracycline revealed that calcium sulfate dihydrate (gypsum) did not incorporate tetracycline nor did its isostructural equivalent, calcium hydrogen phosphate dihydrate (brushite). Calcium carbonate and calcium phosphate (apatite) did incorporate tetracycline. The explanation for these different behaviors of calcium can be found in the crystal structure of the respective compounds, namely, whether or not the Ca ion is readily available to react with tetracycline.
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    Expression of parathyroid hormone-related protein in rat articular cartilage (1995)
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    Calcified tissue international 57 (1995), S. 196-200 
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    ISSN: 1432-0827
    Keywords: PTHrP ; Articular cartilage ; Chondrocyte ; Development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Abstract Expression and localization of parathyroid hormone-related protein (PTHrP) in rat articular cartilage during fetal and postnatal periods were investigated by immunohistochemistry and in situ hybridization. PHTrP displayed distinct distribution and intensity of staining at different ages. In fetal (18-day-old) and young (3-week-old) rats, articular chondrocytes expressed abundant PTHrP throughout the entire thickness of cartilage. In contrast, in 60-week-old rats, PTHrP was expressed in a few articular chondrocytes of superficial and middle layers. Regulation of PTHrP and PTH/PTHrP receptor mRNA was also studied in cultured rat articular chondrocytes. Northern blot analysis revealed that both transforming growth factor-β (TGF-β), an important stimulator for chondrocyte proliferation and differentiation, and 10% fetal bovine serum (FBS) stimulated the expression of PTHrP mRNA with down-regulation of its receptor mRNA. In contrast, 12-O-tetradecanoylphorbol-13-acetate (TPA) down-regulated the expression of receptor without changes of PTHrP mRNA level. These results suggest that the changes in abundance and localization of PTHrP and its receptor may be directly involved in the cell growth and differentiation of articular cartilage.
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    URL: http://dx.doi.org/10.1007/BF00310258
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    Alkaline and acid phosphatase activities during growth of long bones and mandibles (1972)
    Springer
    Calcified tissue international 9 (1972), S. 173-178 
    Details
    ISSN: 1432-0827
    Keywords: Phosphatase ; Development ; Bone ; Growth ; Rhythm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Description / Table of Contents: Résumé Une étude longitudinale, par séries, est effectuée pour déterminer les activités en phosphatases acide et alcaline dans les os longs et la mandibule. Le pH optimum des deux enzymes se situe respectivement à 10.2 et 5.4 pour les phosphatases alcaline et acide. Des portées synchronisées de rats sont sacrifiées, à raison d'une portée par jour, en commençant au premier jour jusqu'au 25ème jour post-partum. Les spécimens sont analysés en ce qui concerne leur concentration en protéine et leur activité en phosphatases. Une activité de types élevée et faible est observée au niveau du tissu osseux, ainsi qu'un type d'activité faible en phosphatase alcaline, au cours des pics d'activité en phosphatase acide, et vice-versa. Les pics observés suggèrent une concordance entre l'activité en phosphatase et les autres changements biochimiques de la croissance osseuse, au niveau de la matrice organique et la formation minérale. Une étude séparée, tenant compte de la possibilité d'une activité enzymatique rythmique, suggère l'existence d'un rythme diurne court chez les animaux jeunes.
    Abstract: Zusammenfassung Eine serienmäßige Longitudinaluntersuchung wurde unternommen, um die Aktivitäten der alkalischen und sauren Phosphatase in den Röhrenknochen und den Mandibulae von Ratten zu bestimmen. Das pH-Optimum der beiden Enzyme wurde für die alkalische Phosphatase bei 10,2 und für die saure Phosphatase bei 5,4 ermittelt. Synchronisierte und randomisierte Würfe wurden getötet, 1 Wurf pro Tag vom 1.–25. Tag post partum. Die Proben wurden auf ihren Proteingehalt und ihre Phosphatasenaktivität untersucht. Ein Muster niedriger und hoher Aktivität konnte in beiden Knochengeweben beobachtet werden, sowie ein Muster von niedriger Aktivität der alkalischen Phosphatase bei Spitzenwerten der sauren Phosphatase und umgekehrt. Die beobachteten Spitzenwerte lassen einen Zusammenhang vermuten zwischen der Phosphatasenaktivität und den anderen biochemischen Veränderungen, die im wachsenden Knochen auftreten, d. h. Bildung der organischen Matrix und des Minerals. Eine getrennte Untersuchung, welche sich mit der Möglichkeit rhythmischer Merkmale der Enzymaktivität befaßte, läßt vermuten, daß in den ersten Tagen ein schwacher Tagersrhythmus bestehen könnte.
    Notes: Abstract A serial longitudinal study was undertaken to determine the activities of the alkaline and acid phosphatases in the long bones and mandibles. The optimum pH of the two enzymes was recorded at 10.2 and 5.4 for alkaline and acid phosphatase, respectively. Synchronized and randomized litters of rats were killed, 1 litter daily, starting at day 1 to day 25 post partum. Samples were analyzed for protein concentration and activity of the phosphatases. A pattern of low and high activity was observed in both bony tissues, as well as a pattern of low alkaline phosphatase activity during acid phosphatase activity peaks, and vice versa. The observed peaks suggest a correspondence between phosphatase activity and the other biochemical changes occurring in the growing bone, i.e., organic matrix and mineral formation. A separate study, considering the possibility of rhythmic features of the enzyme activity suggests that there may be a small diurnal rhythm at an early age.
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    Structure and developmental expression of Ikaros in the Mexican axolotl (1999)
    Springer
    Immunogenetics 50 (1999), S. 336-343 
    Details
    ISSN: 1432-1211
    Keywords: Key words Amphibian ; Axolotl ; Ikaros ; Hematopoiesis ; Development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  TheIkaros family of transcription factors plays an essential role in hematopoiesis. We report here the structure of cDNA clones encoding two Ikaros isoforms, Ik1 and Ik2, in the Mexican axolotl. The Ik1 cDNA sequence is very similar to that of the rainbow trout, chicken, and mammalian Ik1 sequences. However, a 96 base pair region which encodes the first N-terminal zing finger (F1) is lacking from axolotl Ik1, both in clones from a cDNA library and clones isolated from direct polymerase chain reaction products. A region corresponding to exon 3 is completely absent from the axolotl Ik2 sequence and thus the Ik1 and Ik2 isoforms possess the same number of zinc finger motifs. The structure of these five CC-HH motifs is very well conserved in the axolotl, including the structural deviations from its amino acid consensus composition which are identical in all species analyzed to date. The axolotl Ik1 3′ untranslated region sequence is very long (2538 bp) and contains two UA-rich motifs known as instability determinants and which could play a role in mRNA translational efficiency. Ikaros transcripts are first detected in the ventral blood island of stage 36 embryos, about 24 h before the first heartbeats (late tailbud stage), and then in the major lymphopoietic organs of the developing larvae. In situ hybridization demonstrates that Ikaros transcripts are abundant at the periphery of the thymus lobes, in the presumptive site of early thymocyte differentiation.
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    Wide tissue distribution of axolotl class II molecules occurs independently of thyroxin (1998)
    Springer
    Immunogenetics 47 (1998), S. 339-349 
    Details
    ISSN: 1432-1211
    Keywords: Key words Axolotl ; Salamander ; Metamorphosis ; Development ; Class II
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract  Unlike most salamanders, the Mexican axolotl (Ambystoma mexicanum) fails to produce enough thyroxin to undergo anatomical metamorphosis, although a “cryptic metamorphosis” involving a change from fetal to adult hemoglobins has been described. To understand to what extent the development of the axolotl hemopoietic system is linked to anatomical metamorphosis, we examined the appearance and thyroxin dependence of class II molecules on thymus, blood, and spleen cells, using both flow cytometry and biosynthetic labeling followed by immunoprecipitation. Class II molecules are present on B cells as early as 7 weeks after hatching, the first time analyzed. At this time, most thymocytes, all T cells, and all erythrocytes lack class II molecules, but first thymocytes at 17 weeks, then T cells at 22 weeks, and finally erythrocytes at 26–27 weeks virtually all bear class II molecules. Class II molecules and adult hemoglobin appear at roughly the same time in erythrocytes. These data are most easily explained by populations of class II-negative cells being replaced by populations of class II-positive cells, and they show that the hemopoietic system matures at a variety of times unrelated to the increase of thyroxin that drives anatomical metamorphosis. We found that administration of thyroxin during axolotl ontogeny does not accelerate or otherwise affect the acquisition of class II molecules, nor does administration of drugs that inhibit thyroxin (sodium perchlorate, thiourea, methimazole, and 1-methyl imidazole) retard or abolish this acquisition, suggesting that the programs for anatomical metamorphosis and some aspects of hemopoietic development are entirely separate.
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    Food deprivation during different periods of tadpole (Hyla chrysoscelis) ontogeny affects metamorphic performance differently (1995)
    Springer
    Oecologia 103 (1995), S. 518-522 
    Details
    ISSN: 1432-1939
    Keywords: Tadpole ; Development ; Feeding ; Metamorphosis ; Starvation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Tadpoles in small, ephemeral pools whose duration and food content are unpredictable can potentially encounter substantial variation in diet composition and availability. We compared the effects of 10 days of food deprivation occurring early, midway and late in ontogeny on the metamorphic size and bioenergetic properties of Hyla chrysoscelis tadpoles. Tadpoles fed throughout ontogeny were controls. Metamorphs from tadpoles starved early and midway in ontogeny had the same snout-vent length and dry mass as controls, but the time to metamorphosis was extended by 8 and 19% respectively. Metamorphs of tadpoles starved late in development attained 85% of the length and 55% of the mass of controls, metamorphosed at the same time as controls, and suffered mortality 15 times greater than other treatments, perhaps because they were near the absolute minimum necessary level of energy reserves. There were no significant differences in percent organic matter, percent tissue water, condition index, and protein or glycogen concentrations between any experimental and control treatments. If food deprivation occurred early in development, the tadpoles caught up to the size of controls, but an extended developmental time would increase the risk of predation or habitat loss. If food reductions occur late in development, perhaps magnified by pond desiccation, tadpoles are stimulated to metamorphose at the same time as controls but at a smaller size. The bioenergetic composition of tadpoles at metamorphosis is unaffected by time of food deprivation.
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    Simultaneous effects of night-time temperature and an allelochemical on performance of an insect herbivore (1995)
    Springer
    Oecologia 104 (1995), S. 225-233 
    Details
    ISSN: 1432-1939
    Keywords: Development ; Global warming ; Nutritional ecology ; Plant-herbivore interactions ; Rutin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract One effect of global warming may be an increase in night-time temperatures with daytime temperatures remaining largely unchanged. We examined this potential effect of global warming on the performance of tobacco hornworm larvae, Manduca sexta (Sphingidae), by manipulating night-time temperature and dietary rutin levels simultaneously under a 12 light:12 dark photoregime. All four thermal regimes (26:14, 26:18, 26:22, and 26:26° C) had a daytime temperature of 26° C, with the night-time temperature increased from 14 to 26° C by increments of 4° C. Dietary rutin levels (0, 10 and 20 μmoles g−1 fresh weight of diet) reflected those occurring naturally in the leaves of tomato, a preferred host plant of M. sexta. With low night-time temperatures (14 and 18° C), rutin had a negative linear effect on developmental rate, relative growth rate and relative consumption rate of the caterpillars. However, at a night-time temperature of 22° C, rutin had a negative non-linear effect. At a night-time temperature of 26° C, rutin had a negative linear impact but less so than at the other nightime temperatures. Likewise, the negative effect of rutin on molting duration was mitigated as night-time temperature increased. Final larval weight decreased linearly with increased dietary rutin concentrations. Total amount of food ingested was not affected by either rutin or thermal regime. As expected, the caterpillars developed faster under an alternating 26:14° C regime than a constant 20° C regime (the average temperature for the alternating regime), but the effect of rutin depended on the thermal regime. Switching daytime and night-time temperatures had no statistically significant effect on caterpillar performance. Overall, the effect of rutin on rates of larval performance was greater at some levels of warmer nights but damped at another level. These results indicate that the potential effect of warmer nights on insect performance is not a simple function of temperature because there can be interactions between night-time temperature and dietary allelochemicals.
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    Altitudinal life history variation in the dung flies Scathophaga stercoraria and Sepsis cynipsea (1997)
    Springer
    Oecologia 109 (1997), S. 342-352 
    Details
    ISSN: 1432-1939
    Keywords: Key words Body size ; Development ; Phenology ; Phenotypic plasticity ; Season length
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Field phenologies of high- (ca. 1500 m) and low- (ca. 500 m) altitude populations of the two most common European species of dung flies, Scathophaga stercoraria and Sepsis cynipsea, differ quite markedly due to differences in climate. To differentiate genetic adaptation due to natural selection and phenotypic plasticity, I compared standard life history characters of pairs of high- and low-altitude populations from three disjunctive sites in Switzerland in a laboratory experiment. The F1 rearing environment did not affect any of the variables of the F2 generation with which all experiments were conducted; hence, there were no carry-over or maternal effects. In Sc. stercoraria, high-altitude individuals were smaller but laid larger eggs; the latter may be advantageous in the more extreme (i.e. more variable and less predictable) high-altitude climate. Higher rearing temperature strongly decreased development time, body size and the size difference between males and females (males are larger), produced female-biased sex ratios and led to suboptimal adult emergence rates. Several of these variables also varied among the three sites, producing some interactions complicating the patterns. In Se. cynipsea, high-altitude females were marginally smaller, less long-lived and laid fewer clutches. Higher rearing temperature strongly decreased development time and body size but tended to increase the size difference between males and females (males are smaller); it also increased clutch size but decreased physiological longevity. Again, interpretation is complicated by variation across sites and some significant interactions. Overall, genetic adaptation to high-altitude conditions appears weak, probably prevented by substantial gene flow, and may be swamped by the effects of other geographic variables among populations. In contrast, phenotypic plasticity is extensive. This may be due to selection of flexible, multi-purpose genotypes. The results suggest that differences in season length between high- and low-altitude locations alone do not explain well the patterns of variation in phenology and body size.
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    Auxin-induced changes in cell wall extensibility of maize roots (1998)
    Springer
    Planta 204 (1998), S. 515-519 
    Details
    ISSN: 1432-2048
    Keywords: Key words: Acid-growth theory (roots) ; Auxin ; Cell wall extensibility ; Rheology ; Root growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract. The rheological properties of corn (Zea mays L. cv. Garant) root elongation zones were investigated by means of a computer-controlled extensiometer. Creep closely followed a logarithmic time function, which was used to quantify creep activity. Pretreatment with auxin, which inhibits extension growth in roots, lowered the creep activity and the apparent plastic extensibility. While the time course of the inhibition of apparent plastic extensibility lagged behind the cessation of elongation growth, the drop in creep activity matched the growth inhibition more closely. Creep activity and apparent plastic extensibility were not significantly affected by pH. These data support the view that the auxin-induced cell wall stiffening (e.g. by cross-linking processes), while causal for the growth inhibition, is not brought about by a cell wall alkalinization.
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    URL: http://dx.doi.org/10.1007/s004250050286
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    Altered expression of flowering class B and class C genes in the Appendix tobacco mutant (1998)
    Springer
    Sexual plant reproduction 11 (1998), S. 140-147 
    Details
    ISSN: 1432-2145
    Keywords: Key words Nicotiana tabacum ; Flowering ; Mutant ; Carpelloidy ; Development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  In the tobacco (Nicotiana tabacum) Appendix mutant, anthers are tipped by a miniature style and stigma. The outgrowth appears on the anther when it is already differentiating and follows the developmental timing of the central carpel. The Appendix mutation thus represents a late homeotic transformation suggesting that the APPENDIX (APX) gene either could be a misregulated organ identity gene or could be involved in regulating the expression of such genes. RFLP analysis with two class B (TM6 and NTGLO) and a class C (NAG) probes revealed that the Appendix phenotype is not caused by a mutation in one of these genes. However, in situ hybridization showed important changes in the expression of NTGLO and NAG in the mutant when compared with wild-type tobacco. Surprisingly, although no phenotypic alteration other than the style and stigma outgrowth is observed in the Appendix mutant, changes in class B and class C gene expession were not restricted to the anther tip cells from which the outgrowth originates. As expected, NAG was expressed in the Appendix outgrowth but it was also overexpressed in the normal third and fourth whorl organs at the time the outgrowth, as well as the central styles and stigmas, differentiated. Overexpression of a class C gene is probably responsible for the Appendix phenotype. In normal and mutant flowers, NTGLO was expressed in the second, third and fourth whorls up to the time of carpel fusion. Expression of this class B gene then ceased in the fourth whorl organs but was reactivated at later stages only in the styles and stigmas as well as in the outgrowths of the mutant. It thus seems that the function of the APX gene is either to regulate the late expression of organ identity genes or to control cell proliferation in such a way that, in the mutant, some cells are in a state where they respond in an unusual way to developmental signals.
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    URL: http://dx.doi.org/10.1007/s004970050131
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    Development of endosperm in Arabidopsis thaliana (1999)
    Springer
    Sexual plant reproduction 12 (1999), S. 32-42 
    Details
    ISSN: 1432-2145
    Keywords: Key words Arabidopsis thaliana ; Alveoli ; Development ; Endosperm ; Microtubules ; Seeds
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The process of endosperm development in Arabidopsis was studied using immunohistochemistry of tubulin/microtubules coupled with light and confocal laser scanning microscopy. Arabidopsis undergoes the nuclear type of development in which the primary endosperm nucleus resulting from double fertilization divides repeatedly without cytokinesis resulting in a syncytium lining the central cell. Development occurs as waves originating in the micropylar chamber and moving through the central chamber toward the chalazal tip. Prior to cellularization, the syncytium is organized into nuclear cytoplasmic domains (NCDs) defined by nuclear-based radial systems of microtubules. The NCDs become polarized in axes perpendicular to the central cell wall, and anticlinal walls deposited among adjacent NCDs compartmentalize the syncytium into open-ended alveoli overtopped by a crown of syncytial cytoplasm. Continued centripetal growth of the anticlinal walls is guided by adventitious phragmoplasts that form at interfaces of microtubules emanating from adjacent interphase nuclei. Polarity of the elongating alveoli is reflected in a subsequent wave of periclinal divisions that cuts off a peripheral layer of cells and displaces the alveoli centripetally into the central vacuole. This pattern of development via alveolation appears to be highly conserved; it is characteristic of nuclear endosperm development in angiosperms and is similar to ancient patterns of gametophyte development in gymnosperms.
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    URL: http://dx.doi.org/10.1007/s004970050169
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    Sex organ determination and differentiation in the dioecious plant Melandrium album (Silene latifolia): a cytological and histological analysis (1997)
    Springer
    Sexual plant reproduction 10 (1997), S. 155-167 
    Details
    ISSN: 1432-2145
    Keywords: Key words Dioecious plant ; Melandrium album ; Development ; Sex determination ; Organ differentiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Melandrium album (Silene alba) is a dioecious plant with heteromorphic sex chromosomes (XY system). Sexual dimorphism is a result of developmental blocks in male or female reproductive organ formation within young bipotential flower buds. Progress in understanding the genetic and molecular mechanisms controlling sex determination in this species relies on a detailed description of developmental timing in the two sexes, with emphasis on those early stages during which sexual dimorphism is established. We used a combination of histological and scanning electron microscopy analysis to refine the comparative study and description of the staging of male compared to female flower development. We show that (1) female dimorphism results from modifications in flower meristem organisation, namely a sudden arrest of cell divisions in whorl 4 of male flowers at the time when meristem partitioning is achieved between whorls 3 and 4, and (2) male dimorphism is part of the stamen differentiation process corresponding to stamen arrest at the early sporogenous stage in female flowers. Thus, Melandrium is a natural double ”mutant” that is affected in very early and distinct processes of reproductive organ differentiation. Our results are used to discuss the most likely nature of the specific functions controlling sexual dimorphism in Melandrium.
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    URL: http://dx.doi.org/10.1007/s004970050083
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    Tissue-specific and differential editing of the two ycf3 editing sites in maize plastids (1997)
    Springer
    Current genetics 32 (1997), S. 19-23 
    Details
    ISSN: 1432-0983
    Keywords: Key words RNA editing ; C-to-U editing ; IRF170 ; Development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The hypothetical chloroplast open reading frame 3 (ycf3) of maize, consisting of three exons and two group II introns, contains two editing sites. Both of these sites were investigated with respect to the extent of editing in various tissues and different developmental stages. Northern blot analyses show nearly identical transcript patterns of ycf3 in all tissues investigated. In leaf plastids, both editing sites are completely edited, independent of light conditions and developmental stage. In non-leaf plastids, however, one editing site of ycf3 is only partially edited in unspliced transcripts and in one type of partially spliced transcripts. In different developmental stages of the same tissue, on the other hand, no differences in editing efficiency were found. These results indicate that, in partially spliced transcripts, different editing sites of one and the same gene can be edited with different efficiencies in a tissue-specific manner.
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    URL: http://dx.doi.org/10.1007/s002940050242
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    catA, a newAspergillus nidulans gene encoding a developmentally regulated catalane (1996)
    Springer
    Current genetics 29 (1996), S. 352-359 
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    ISSN: 1432-0983
    Keywords: Aspergillus ; Catalase ; Oxidative stress ; Sporulation ; Spore resistance ; Development ; Gene regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Aspergillus nidulans asexual sporulation (conidiation) is a model system for studying gene regulation and development. The CAN5 cDNA is one of several clones isolated based on transcript induction during conidiation. Here we present the molecular characterization of its corresponding gene, demonstrating that it encodes a developmentally regulated catalase, designatedcatA. ThecatA 744-amino-acid-residue polypeptide shows significant identity to other catalases. Its similarity to prokaryotic catalases is greater than to other fungal catalases.catA mRNA is barely detectable in growing mycelia, highly induced during sporulation, and present in isolated spores. However,catA expression is not dependent on the developmental regulatory genesbrlA, abaA andwetA. Direct catalase activity determination in native gels revealed the existence of two bands of activity. One of these bands represented the major activity during vegetative growth and was induced during sporulation. The second catalase activity appeared after the induction of sporulation and was the predominant activity in spores. Disruption ofcatA abolished the major spore catalase without eliminating the vegetative activity, indicating the existence of at least two catalase genes inA. nidulans.catA-disrupted mutants produced spores that were sensitive to H2O2, as compared to wild-type spores. The increase in the activity of the vegetative catalase and the appearance of a second catalase during asexual sporulation is consistent with the occurrence of an oxidative stress during development.
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    URL: http://dx.doi.org/10.1007/BF02208616
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    catA, a new Aspergillus nidulans gene encoding a developmentally regulated catalase (1996)
    Springer
    Current genetics 29 (1996), S. 352-359 
    Details
    ISSN: 1432-0983
    Keywords: Key words  Aspergillus ; Catalase ; Oxidative stress ; Sporulation ; Spore resistance ; Development ; Gene regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract   Aspergillus nidulans asexual sporulation (conidiation) is a model system for studying gene regulation and development. The CAN5 cDNA is one of several clones isolated based on transcript induction during conidiation. Here we present the molecular characterization of its corresponding gene, demonstrating that it encodes a developmentally regulated catalase, designated catA. The catA 744-amino-acid-residue polypeptide shows significant identity to other catalases. Its similarity to prokaryotic catalases is greater than to other fungal catalases. catA mRNA is barely detectable in growing mycelia, highly induced during sporulation, and present in isolated spores. However, catA expression is not dependent on the developmental regulatory genes brlA, abaA and wetA. Direct catalase activity determination in native gels revealed the existence of two bands of activity. One of these bands represented the major activity during vegetative growth and was induced during sporulation. The second catalase activity appeared after the induction of sporulation and was the predominant activity in spores. Disruption of catA abolished the major spore catalase without eliminating the vegetative activity, indicating the existence of at least two catalase genes in A. nidulans. catA-disrupted mutants produced spores that were sensitive to H2O2, as compared to wild-type spores. The increase in the activity of the vegetative catalase and the appearance of a second catalase during asexual sporulation is consistent with the occurrence of an oxidative stress during development.
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    URL: http://dx.doi.org/10.1007/s002940050056
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    Growth regulation inCoprinus radiatus (1974)
    Springer
    Archives of microbiology 96 (1974), S. 353-364 
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    ISSN: 1432-072X
    Keywords: Coprinus ; Agaricaceae ; Mushroom ; Development ; Growth Regulator ; Stipe Growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Some of the morphological and physiological parameters of stipe growth or elongation inCoprinus radiatus were investigated. During the development of the fruit body the number of cells in a row in the growing portion of the stipe doubled during the development of the button, and again during the phase of rapid stipe elongation. Also during the stage of rapid elongation the cells in the upper 2/3 of the stipe increased 6–8 fold in length. The existence of a growth regulator synthesized in the cap and exerting control over the stipe was demonstrated through decapitation experiments. The cap appears to be required for normal stipe development until the stipe reaches about 1/4 of its final length. Through decapitation and cap-stipe exchanges it was found that the cap produced growth regulator up to the time of autodigestion; however, the stipe responded to the regulator only during a brief period at the onset of elongation.
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    URL: http://dx.doi.org/10.1007/BF00590190
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    A study of sporophore development in the myxomyceteProtophysarum phloiogenum (1974)
    Springer
    Archives of microbiology 99 (1974), S. 331-344 
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    ISSN: 1432-072X
    Keywords: Myxomycetes ; Ultrastructure ; Development ; Systematics ; Food Vacuoles ; Stalk Development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Observations of sporophore development in fresh and glutaraldehydeosmium sequentially-fixed material ofProtophysarum phloiogenum show the following sequence. Small plasmodia cease streaming and round up. Food vacuoles collect in the lower center of the cytoplasmic mass. As the cytoplasm rises the food vacuolar contents are excluded from the plasmalemma and become the stalk core. A continuous, fibrous peridium and stalk tube enclose cytoplasm and stalk core respectively. Capillitial formation just precedes spore cleavage. Sporophore development is marked by autophagic activity and calcium deposition. Stalks of dried herbarium specimens of seven additional species have been examined. A mature stalk morphology very similar toProtophysarum with recognizable remnants of microorganismal food material is seen in all of them. It is thought that this marker is indicative of non-stemonitaceous stalk development.
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    URL: http://dx.doi.org/10.1007/BF00696247
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    Die Bedeutung von Adenosin-3′,5′-monophosphat für die entwicklung vonAcanthamoeba castellanii (1974)
    Springer
    Archives of microbiology 99 (1974), S. 155-166 
    Details
    ISSN: 1432-072X
    Keywords: cAMP ; Acanthamoeba ; Development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung Durch drei verschiedene Methoden wurde in Kulturen vonAcanthamoeba castellanii die stationäre Wachstumsphase, in deren Verlauf sich Trophozoiten zu Cysten entwickeln, induziert: Durch Nahrungsmangel, indem Amöben der logarithmischen Wachstumsphase in ein nährstofffreies Medium überführt wurden, durch Sauerstoffmangel, indem Kulturen zu großer Zelldichte heranwuchsen, und durch Hemmung der mitochondrialen DNS-Synthese, indem Kulturen der logarithmischen Phase mit Äthidiumbromid versetzt wurden. Unabhängig von den Encystierungsbedingungen nimmt die intracelluläre Konzentration von Adenosin-3′,5′-monophosphat (cAMP) bei Verminderung der Zellteilungsrate bis zu Beginn der stationären Wachstumsphase um das 2–3 fache zu. Die Ergebnisse deuten an, daß der Anstieg von intracellulärem cAMP auf eine Erhöhung der Adenylat-Cyclase- und nicht auf eine Verminderung der Phosphodiesterase-Aktivität zurückzuführen ist. Extracelluläres cAMP konnte weder in Kulturen der logarithmischen noch in Kulturen der stationären Phase nachgewiesen werden. Dies ist vermutlich auf die extracellulär vorhandene cAMP-Phosphodiesterase zurückzuführen. Die Tatsache, daß auch durch Theophyllin die stationäre Wachstumsphase und Encystierung induziert werden kann, läßt auf eine Beteiligung von cAMP an den Entwicklungsprozessen, speziell an dem Abbau von Glykogen, schließen.
    Notes: Abstract In cultures ofacanthamoeba castellanii the stationary growth phase in which trophozoites develop to cysts, was induced in three ways: by transferring cells from a logarithmic growing culture into a nutrient-free medium, by growth in nutrient medium to high cell density and by inhibition of the mitochondrial DNA synthesis with ethidium bromide. In all cases, the intracellular concentration of adenosine-3′,5′-monophosphate (cAMP) rises by a factor of two to three from the end of the logarithmic phase to the beginning of the stationary phase. The results show that this rise may be more a consequence of an increased adenylate cyclase activity than of a diminished cAMP phosphodiesterase activity. No extracellular cAMP could be measured in cultures of the logarithmic and stationary growth phase, perhaps because of the extracellular cAMP phosphodiesterase. Because theophylline also induces the stationary phase and encystation, cAMP seems to be of importance for the development ofAcanthamoeba castellanii, especially for the degradation of glycogen.
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    URL: http://dx.doi.org/10.1007/BF00696231
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    Wyoming's industrial siting permit process and environmental impact assessment (1982)
    Springer
    Environmental management 6 (1982), S. 1-7 
    Details
    ISSN: 1432-1009
    Keywords: Natural resource planning ; Development ; Policy analysis ; Industrial siting ; Environmental impact assessment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract The problem of management of industrial residuals can be reduced through a rational system for siting and planning major industrial facilities. In the United States, Wyoming has moved in the direction of establishing a one-stop permitting system that provides important information for air and water quality planning and solid waste management with a minimum of regulatory overlap. This paper describes Wyoming's Industrial Development Information and Siting Act of 1975 and suggests ways in which the Wyoming permitting system can be improved and applied elsewhere.
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    URL: http://dx.doi.org/10.1007/BF01866801
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    Using ecological knowledge for development planning (1980)
    Springer
    Environmental management 4 (1980), S. 13-20 
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    ISSN: 1432-1009
    Keywords: Planning ; Management ; Natural resource planning ; Development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Since October 1977, the East-West Environment and Policy Institute in Honolulu has been conducting a multinational collaborative project to enhance the preparation and utilization of natural systems assessments in developing countries. This paper presents some of the findings to date: 1. Channels are developing rapidly for transferring ecological knowledge into political and administrative decision making. 2. The systematic approach of ecology is replacing “environmental quality” as the organizing concept for information about natural resources and the environment. 3. Benefit-cost analysis is a promising method for integrating ecological knowledge into economic development decision making. 4. The lack of baseline information, inventories, and predictive capability will not be remedied soon or easily; thus priorities for ecological research are essential.
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    URL: http://dx.doi.org/10.1007/BF01866216
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    A photosensitive circadian oscillator in an insect endocrine gland: photic induction of rhythmic steroidogenesis in vitro (1998)
    Springer
    Journal of comparative physiology 182 (1998), S. 343-349 
    Details
    ISSN: 1432-1351
    Keywords: Key words Pacemaker ; Entrainment ; Ecdysteroids ; Prothoracicotropic hormone ; Development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The paired prothoracic glands of the insect Rhodnius prolixus each comprise a group of about 200 structurally identical cells. The synthesis (and release) of steroid moulting hormones (ecdysteroids) by these glands is under circadian control in vivo. We monitored ecdysteroid synthesis by single glands during long-term incubations in vitro. Synthesis is rhythmic in vitro and persists in continuous darkness. Glands which are arrhythmic (from prolonged continuous light) respond to transfer to darkness in vitro with the initiation of a free-running circadian rhythm of ecdysteroid synthesis. Therefore, the glands possess a light-sensitive circadian oscillator. These properties are conventionally associated with nervous tissue of animals. It is suggested that rhythmicity is synchronized within the gland by the known structural and electrical coupling between its component cells. The glands share properties with known pacemakers such as the avian pineal. However, the glands in vivo receive input from both light cues and the cerebral neuropeptide, prothoracicotropic hormone. Rhythmic release of this neuropeptide is controlled by a second oscillator located in the brain. We conclude that the pacemaker in the endocrine system of R. prolixus comprises at least three oscillators, one in each prothoracic gland and one in the brain, which are coupled hormonally. We conclude that the prothoracic gland is an important component of the circadian system controlling development in R. prolixus and that peripheral endocrine glands may play a more active role in the generation of animal circadian organization than has been thought.
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    Viability of Rhizobium leguminosarum endosymbionts in different stages of development (1981)
    Springer
    Archives of microbiology 129 (1981), S. 349-352 
    Details
    ISSN: 1432-072X
    Keywords: Rhizobium ; Bacteroids ; Viability ; Development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Viability of Rhizobium leguminosarum bacteroids isolated from Pisum sativum was determined after sucrose gradient centrifugation of the bacteroids resulting in separation according to developmental stage. The results suggest that past a critical stage of development bacteroids lose viability. Similar experiments with R. japonicum bacteroids having a lower degree of morphological differentiation showed an appreciably higher viability in all gradient fractions. The results support the hypothesis that bacteroid morphology and viability is dependent on the nature of—and length of exposure to—the host plant cytoplasm.
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    Das Adenosinphosphat-System während Wachstum und Entwicklung von Acanthamoeba castellanii (1974)
    Springer
    Archives of microbiology 101 (1974), S. 391-399 
    Details
    ISSN: 1432-072X
    Keywords: Adenosinephosphate System ; Energy Charge ; Acanthamoeba ; Development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung Die Konzentration von Adenosintri-, Adenosindi- und Adenosinmonophosphat wurde in Acanthamoeba castellanii währed der logarithmischen Wachstumsphase und der stationären Wachstumsphase, in deren Verlauf sich Trophozoiten zu Cysten entwickeln, bestimmt. Der Entwicklungsprozess wurde durch drei verschiedene Methoden hervorgerufen: Durch Wachstum im Nährmedium zu großer Zelldichte, durch Überführen von Amöben der logarithmischen Wachstumsphase in ein nährstoffreies Salzmedium und durch Versetzen logarithmisch wachsender Amöben mit Äthidiumbromid. In allen Fällen wird der Adenosinphosphat-Gehalt in den Zellen im Laufe der Entwicklung um etwa 85% reduziert, wozu besonders die Abnahme des ATP-Gehaltes beiträgt. Die Adenosinphosphat-“energy charge” beträgt in logarithmisch wachsenden Amöben 0,83. Im Laufe der Entwicklung wird sie je nach Encystierungsbedingungen auf unterschiedlichen Werten stabilisiert (zwischen 0,58 und 0,81). Die Möglichkeit eines Zusammenhanges von Konzentrationsveränderungen der Adenosinphosphate und entwicklungsspezifischen Prozessen wird diskutiert.
    Notes: Abstract The concentration of adenosine tri-, adenosine di-, and adenosine monophosphate in cells of Acanthamoeba castellanii was measured during the logarithmic growth phase and the stationary growth phase in which trophozoites were transformed into cysts. This developmental process was induced in three ways: by growth in nutrient medium to high cell density, by transferring cells in the logarithmic phase into a nutrient-free medium, and by mixing logarithmically growing cells with ethidium bromide. In all cases, encystment is accompanied with a reduction of total adenosine phosphate content to about 85%, mainly because of a depletion of cellular ATP. The value of the adenosine phosphate energy charge in logarithmically growing amoebae is 0.83. During development the energy charge becomes stabilized at different values (between 0.58 and 0.81), characteristic to the mode of encystation. A possible functional relationship between changes of the adenosine phosphate concentration and developmental processes of the amoeba is discussed.
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    Changes of the cytoplasmic ultrastructure during development of sclerotia in Claviceps purpurea (Fr.) Tul. (1980)
    Springer
    Archives of microbiology 125 (1980), S. 251-257 
    Details
    ISSN: 1432-072X
    Keywords: Claviceps purpurea ; Ultrastructure ; Development ; Sclerotium ; Oleosomes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The development of sclerotia of Claviceps purpurea was investigated by light and electron microscopy. During the first days after infection sterigma and conidiospores are formed. The spores show a moderately developed vacuolar system, they are thick walled and contain about 20% lipid (related to the cell volume) embedded in glycogen. The sterigma are cylindrical unicellular hyphae with electron dense cytoplasm and isolated strongly contrasted lipid droplets. In maturing sclerotia the hyphae become septated with increasingly thick cell walls and a large lipid content. The lipid forms small droplets in young cells, while in the mature sclerotium it occurs in the form of very large drops, occupying the major part of the cell. Simultaneously the composition of the lipid is changed. The mature cells have several nuclei. They are partially connected by osmiophilic substances, forming a network of intercellular spaces.
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    Antheridiol, RNA polymerase II and sexual development in the aquatic fungus, Achlya (1983)
    Springer
    Archives of microbiology 134 (1983), S. 314-319 
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    ISSN: 1432-072X
    Keywords: Antheridiol ; RNA Polymerase II ; Development ; ELISA ; Achlya ; Transcriptional regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The fungus, Achlya, is one of the most primitive eukaryotes known to secrete and respond to diffusible steroid sex hormones (pheromones). Antheridiol, which is produced by female strains of Achlya induces male strains to differentiate male sex organs. Induction of male strains with antheridiol elicits several changes in macromolecular synthesis including a quantitative enhancement in the synthesis of poly adenylated messenger RNA. We have examined whether this quantitative change is due to the regulation of RNA polymerase II. The level of polymerase increases as a response to the pheromone. This was examined using two different approaches, one of which included an extremely sensitive enzyme-linked immunosorbant assay (ELISA). Furthermore, the specific activities of polymerase II preparations isolated from pheromone-stimulated cultures was significantly higher than the specific activities of enzyme preparations isolated from control. A comparison of the polypeptide subunit composition of polymerase II preparations isolated from both pheromone-treated and control cultures on SDS polyacrylamide gels indicated no qualitative differences. Apparent differences in the stoichiometry of two specific subunits were reproducibly observed. The subunits of 140,000 and of 69,000 stained much more intensely in RNA polymerase II preparations isolated from 4h antheridiol-treated cultures.
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    Control of the formation of bacteriochlorophyll, and B 875- and B 850-bacteriochlorophyll complexes in Rhodopseudomonas sphaeroides mutant strain H5 (1983)
    Springer
    Archives of microbiology 136 (1983), S. 312-316 
    Details
    ISSN: 1432-072X
    Keywords: Rhodopseudomonas sphaeroides ; Continuous culture ; Control ; Bacteriochlorophyll ; Pigment protein complexes ; 5-Aminolevulinic acid ; Development ; Light
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Rhodopseudomonas sphaeroides mutant H5 lacking 5-aminolevulinic acid synthase was employed to study the control of the formation of total bacteriochlorophyll as well as of the B875- and B850-bacteriochlorophyll protein complexes. The organisms were grown phototrophically in a chemostat where cell protein formation was limited by iron ions and bacteriochlorophyll by 5-aminolevulinic acid. 0.07 mol of bacteriochlorophyll was formed per mol of 5-aminolevulinic acid consumed. This stoichiometric relationship was not influenced by a twelve-fold variation in light energy flux. However, cell protein levels increased and, consequently, cellular specific bacteriochlorophyll contents decreased with increases in light energy flux. The ratio of B875- to B850-pigment protein complexes was inversely proportional to the velocity of 5-aminolevulinic acid supply (mol per cell protein and time) which in this system equals the velocity of 5-aminolevulinic acid consumption and the velocity of bacteriochlorophyll formation. Light had no direct effect on the ratio of B875- per B850-pigment complexes but an indirect effect via its control of protein formation. Changes in the ratio of the two pigment complexes resulted from the fact that significantly lower amounts of 5-aminolevulinic acid supplied per protein and time were required to saturate the system assembling the B875-complexes than that assembling the B850-complexes. The data suggest lack of light-dependent control in the formation of bacteriochlorophyll and its complexes subsequent to the 5-aminolevulinic acid pool.
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    URL: http://dx.doi.org/10.1007/BF00425223
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    Stigma development in Nicotiana tabacum. Cell death in transgenic plants as a marker to follow cell fate at high resolution (1996)
    Springer
    Sexual plant reproduction 9 (1996), S. 243-254 
    Details
    ISSN: 1432-2145
    Keywords: Key words Nicotiana tabacum ; Stigma ; Ablation ; Development ; Lipid secretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Pistil development was studied in transgenic tobacco plants in which the stigma is ablated by expression of a stigma-specific cytotoxic gene. These plants offer a tool to investigate the process of differentiation of the secretory zone, in that cell death caused by barnase activity provides a marker to follow cell fate at high resolution. After fusion of the carpel walls in the region most distal from the ovary, the epidermal cells begin to divide in both wild-type and stigmaless plants. Divisions of the L1 layer of the pistil are immediately followed by the morphogenetic events that lead to three different cell types: rounded-angular cells showing an equal number of anti- and periclinal divisions, cells that are more oblong forming the transition zone, and the square cells of the transmitting tissue dividing mostly anticlinally with respect to the original carpel wall. In the stigmaless plants, cell death caused by the expression of STIG 1-barnase begins at stage –1 and proceeds gradually, but is always associated with round epidermal cells and with angular-rounded cells underneath them. Studies at the ultrastructural level show that cell death caused by barnase activity occurs first in solitary cells and gradually extends to groups of cells. In situ hybridizations using the STIG 1 RNA probe in wild-type pistils confirm these results. Most likely, the cells in which STIG 1 is expressed are those that have just differentiated into the secretory cell type. Our results indicate that the transition zone or neck is autonomously differentiated from the secretory zone and the transmitting tissue. Furthermore, our results indicate that in both wild-type and stigmaless pistils secretion of lipids most likely occurs through the plasmodesmata. This observation suggests that bulk transport can occur via plasmodesmata.
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    URL: http://dx.doi.org/10.1007/s004970050039
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    Glandular scale development and essential oil secretion in Origanum dictamnus L. (1982)
    Springer
    Planta 156 (1982), S. 496-504 
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    ISSN: 1432-2048
    Keywords: Development ; Glandular scale ; Origanum dictamnus ; Secretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Glandular scales of Origanum dictamnus L. originate from a single protodermal cell. They are composed of a 12-celled head and an unicellular stalk and foot. During the early stages of gland differentiation, the head cells possess a small number of plastids which contain globular inclusions. Similar inclusions are also observed in the plastids of the stalk and the foot cell. The lateral walls of the stalk cell progressively undergo cutinization which does not extend to the upper and lower periclinal walls. At the onset of secretion the electron density of the plasmalemma region lining the apical walls of the head cells remarkably increases. These walls are impregnated with an osmiophilic substance identical in appearance to the content of the subcuticular space. In a following stage of the secretory process osmiophilic droplets of various size arise in the cytoplasm of the secretory cells which undergoes simultaneously a reduction of its initial density. After secretion has been concluded the protoplast of the head cells becomes gradually degenerated. The chlorenchyma cells of the mesophyll possess numerous microbodies closely associated with various organelles. In the cytoplasm of these cells crystalloids occasionally occur.
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    Convergence or divergence? The impact of technology on “why growth rates differ” (1995)
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    Journal of evolutionary economics 5 (1995), S. 269-284 
    Details
    ISSN: 1432-1386
    Keywords: Technology ; Growth ; Development ; O1 ; O3 ; O4
    Source: Springer Online Journal Archives 1860-2000
    Topics: Economics
    Notes: Abstract This paper presents an overview and assessment of the theoretical and empirical work on catch-up and growth, with particular emphasis on the impact of technology, and the consequences for developing countries. The point of departure is the neoclassical theory of economic growth, as laid out by Solow and others in the 1950s, and the applied work that followed (“growth accounting”). Then the contributions from economic historians and more heterodox economists, such as Schumpeter, Kaldor and others, are discussed, followed by an account of the most recent theoretical developments (“new growth theory”) and the empirical (econometric) work in this area.
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    Novel mutations affecting leaf stearate content and plant size in Arabidopsis (1997)
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    Theoretical and applied genetics 94 (1997), S. 975-981 
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    ISSN: 1432-2242
    Keywords: Key words Arabidopsis ; Fatty acid ; Suppressor ; Development ; Mutant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The fab2-1 mutant of Arabidopsis is an extreme dwarf as a direct result of an increase in the levels of stearate (18 : 0) in membrane lipids. We isolated a series of lines in which second-site suppressor mutations partly alleviate the dwarf phenotype. In all four of the suppressor lines examined, restoration of more normal morphology is accompanied by decreases in leaf 18 : 0 content. Three of the isolated suppressors suppress the high stearate phenotype in both leaves and seeds. The effects of one of the suppressors, TW2-1, is limited to the leaves. A second allele at the fab2 locus, fab2-2, was also identified and plants homozygous for this allele where intermediate in both plant size and 18 : 0 content between wild-type Arabidopsis and fab2-1 mutants. The alleles at fab2 and the suppressor mutations provided a total of nine genotypes which were analyzed to demonstrate a clear-cut relationship between leaf 18 : 0 content (0.7–19.6% of total leaf fatty acids) and reductions in plant size (24–4 mm). These results illustrate the utility of suppressor analysis for addressing problems in biochemistry and plant biology. They also indicate that the genetic control of plant lipid composition is more complex than previously appreciated.
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    URL: http://dx.doi.org/10.1007/s001220050504
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    Over-expression of the oat arginine decarboxylase cDNA in transgenic rice (Oryza sativa L.) affects normal development patterns in vitro and results in putrescine accumulation in transgenic plants (1998)
    Springer
    Theoretical and applied genetics 97 (1998), S. 246-254 
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    ISSN: 1432-2242
    Keywords: Key words Transgenic plants ; Development ; Oryza sativa ; Arginine decarboxylase ; Polyamines
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Transgenic rice (Oryza sativa L.) cell lines and plants expressing an oat arginine decarboxylase (Adc) cDNA under the control of the CaMV 35 S promoter were recovered using particle bombardment. Molecular analyses confirmed stable integration of the transgene and active transcription (mRNA). A four- to sevenfold increase in arginine decarboxylase (ADC) activity was observed in transformed plants compared to wild-type controls. Biochemical analysis of cellular polyamines (PAs) indicated up to fourfold increase in putrescine (Put) levels in transgenic callus and regenerated plants. This is the first report which demonstrates an increase in PA levels in plants engineered with the Adc gene. Implications of this increase are discussed in terms of development, physiology and nutrition. We observed a correlation between high levels of Adc gene expression and inability of callus tissue to develop normally into differentiated plants. This correlates well with reports in other species, in which perturbation of the PA pathway using genes involved in PA biosynthesis results in aberrant phenotypes. We have shown for the first time that PA biosynthesis can be manipulated in cereal species using genetic engineering.
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    Etudes sur l'épicuticule des insectes (1970)
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    Cell & tissue research 108 (1970), S. 380-396 
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    ISSN: 1432-0878
    Keywords: Epicuticle ; Insects ; Tenebrio molitor ; Development ; Structure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Résumé L'épicuticule de l'adulte de Tenebrio molitor est composée de deux couches distinctes dénommées épicuticule externe et épicuticule interne. L'épicuticule externe est la première couche cuticulaire sécrétée sous forme de petites plaques s'agrandissant par leurs bords pour recouvrir toute la surface cellulaire. Au moment de sa sécrétion, cette couche est formée de quatre lames denses A, B, C1 et C2. La lame B, très fine, disparaît par la suite et les lames C1 et C2 deviennent très nettes. L'épicuticule externe de l'adulte est donc formée de trois lames denses séparées par deux lames claires. L'épicuticule interne est formée de lames superposées denses et claires de structure complexe, qui sont masquées pendant la sécrétion des premières couches de cuticule lamellée (procuticule). Cette structure correspond à un arrangement moléculaire hautement organisé. La forme de la surface cuticulaire des sternites est déterminée par la forme de la surface cellulaire avant le dépôt de l'épicuticule.
    Notes: Summary The epicuticle of adult Tenebrio consists of two distinct layers named outer and inner epicuticle. The outer epicuticle is the first cuticular layer to be deposited in form of small patches on top of the microvilli. These initial patches are composed of four dense laminae (A, B, C1 and C2) separated by three light spaces. The outer epicuticle grows by densification of diffuse material at the edges of the patches until the entire area is covered. The thickness of outer epicuticle remains constant (175 Å) during the development of the pharate adult, lamina B however rapidly disappears. Thus, the adult outer epicuticle is fivelayered (three dense laminae: A, C1 and C2). After being deposited, the inner epicuticle shows a complex laminar structure interpreted to represent a highly organized molecular system. The laminae are masked during the formation of the first procuticle lamellae. During the deposition of the epicuticle, lamina A is covered by a component of the moulting fluid, forming an irregular dense layer which disappears after the resorption of this fluid. Perhaps this layer protects the new epicuticle from lytic enzymes of the moulting fluid. In adult animals, there is an additional superficial layer, the signification of which is not clear. The possibility of remains of cement or wax is discussed. The development of the surface patterns of the sternal and pleural cuticle is determined before the epicuticle formation by the shape of the epidermal surface. The rate of outer epicuticle deposition appears to depend on the size of the microvilli: epicuticle deposition seems to proceed faster over high microvilli.
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    The fine structure and development of the seminal vesicles and prostate in the fetal rat (1970)
    Springer
    Cell & tissue research 109 (1970), S. 1-14 
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    ISSN: 1432-0878
    Keywords: Seminal vesicle ; Prostate ; Fetal rat ; Ultrastructure ; Development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The development of the seminal vesicle from the Wolffian duct and of the prostate from the urogenital sinus has been studied in rat fetuses from day 14 of gestation to birth with the use of the electron microscope. Prior to the onset of androgen secretion, the cells of the urogenital sinus and the caudal part of the Wolffian duct have a simple undifferentiated appearance. After the onset of androgen secretion by the fetal testes at day 15, “intracytoplasmic confronting cisternae” of the granular reticulum appear in both urogenital sinus and Wolffian duct. Portions of the granular endoplasmic reticulum of the urogenital sinus become distended with a finely granular, moderately dense material. In the urogenital sinus, many hemidesmosomes are formed at the basal surface of the epithelium. Specializations of the extracellular materials are present opposite the hemidesmosomes. The formation of the seminal vesicles and the prostate begins at day 18–19 of gestation. The cells of the seminal vesicle are taller than the Wolffian duct cells from which they arise, the granular endoplasmic reticulum increases moderately in amount, and a patent lumen is formed. The cells of the fetal prostate do not differ greatly from those of the urogenital sinus from which they arise except that the prostatic cells initially lack hemidesmosomes. The fine structural changes are discussed in relation to the onset of fetal androgen secretion, the formation of the organs, and the functions of the cells in adult life.
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    Die morphologische Differenzierung des visceralen Blattes der Bowmanschen Kapsel (1970)
    Springer
    Cell & tissue research 111 (1970), S. 346-363 
    Details
    ISSN: 1432-0878
    Keywords: Kidney ; Glomerulus ; Development ; Scanning electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die Differenzierung der Podocyten wurde an Nieren 10 Tage alter Ratten raster-elektronenmikroskopisch untersucht und mit durchstrahlungs-elektronenmikroskopischen Befunden verglichen. Die Podocytenfortsätze können danach auf dreierlei Wegen gebildet werden: 1. Spalten innerhalb des Cytoplasmas lassen bandartige Cytoplasmabrücken entstehen. Diese gliedern sich weiter auf, bis zahlreiche miteinander verzahnte Fortsätze derselben Zelle entstanden sind. 2. Vom Zellrand her werden dicke Fortsätze weit vorgeschoben, die kleinere Fortsätze bilden. Durch sie können Verzahnungen mit entfernten Deckzellen entstehen. Die kleinen Fortsätze können sich jedoch auch mit anderen Fortsätzen der eigenen Zelle verzahnen. 3. Fingerförmige Fortsätze benachbarter Zellen verzahnen sich während ihrer Entstehung miteinander. Trotz zahlreicher desmosomenartiger Haftstellen zwischen benachbarten Podocyten entwickeln sich ihre Fortsätze und deren Verzahnungen anscheinend weitgehend autonom und nur selten nach den vermuteten Regeln epithelialer Nachbarschaft (Typ 3). Die Befunde sprechen vielmehr dafür, daß durchflutete und wachsende Glomerulumkapillaren die Podocytendifferenzierung induzieren und die Orientierung der Fortsätze beeinflussen.
    Notes: Summary The differentiation of the podocytes was studied by scanning electron microscopy on kidneys of 10 days old rats. The results were compared with transmission electron microscopic pictures from the same kidneys. There are three ways of forming processes by the podocytes: 1. Slits within the cytoplasm give rise to cytoplasmic bridges which further divide themselves and finally build up a meshwork of processes within a cell. 2. Thick and sometimes very long processes originate from the cell border. Their smaller branches may interdigitate with those of distant podocytes or with other processes out of the same cell. 3. Finger-like processes of neighbouring cells interdigitate as soon as they develop. In spite of numerous desmosomal structures between neighbouring podocytes the cell processes and their interdigitations develop mostly independently from each other and only seldom after the expected rules of epithelial vicinity (type 3). These findings are interpreted as indication that flooded and growing capillaries induce the differentiation of podocytes and that they influence the orientation of their processes.
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    The structure of the terminal sensilla on the maxillary palps of Locusta migratoria (L.), and changes associated with moulting (1971)
    Springer
    Cell & tissue research 121 (1971), S. 48-68 
    Details
    ISSN: 1432-0878
    Keywords: Chemoreceptor ; Locusta ; Fine structure ; Development ; Moulting
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The basic structure of the terminal sensilla of Locusta migratoria resembles that of Schistocerca gregaria. There are commonly six or ten neurons whose dendrites extend almost to the opening of the peg. Proximally the dendrites are clothed by a neurilemma cell which also encloses a basal cavity through which their ciliary region passes. The tormogen cell encloses the receptor-lymph cavity and actively secretes material into it. The receptor-lymph cavity and the basal cavity are quite separate. The development of new pegs at a moult is described. After apolysis the scolopale extends across the subcuticular space and protects the dendrites, which remain in a functional condition until shortly before ecdysis. As the trichogen cell grows out to form a new peg the tip is surrounded by a mass of electron-dense material, probably derived from the receptorlymph cavity. The function of this material is unknown. Regeneration of the dendrites is considered. The possible mechanism by which the tip of the peg opens and closes is considered and the general structure of the organule is discussed in relation to functioning.
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    BMP/GDF-signalling interactions during synovial joint development (1999)
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    Cell & tissue research 296 (1999), S. 111-119 
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    ISSN: 1432-0878
    Keywords: Key words Synovial joint ; Development ; Hyaluronan ; BMP ; GDF-5 ; Antagonists
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The synovial joint arises from an initial condensation of cells that subsequently develops into distinct skeletal structures, separated by the joint. Bone morphogenetic proteins (BMPs) and growth and differentiation factors (GDFs) have a fundamental role during skeletogenesis, including joint formation. Development of the joint appears to be dependent on the differential expression/activity of the related BMP and GDF subfamilies. Gdf-5 is expressed in the developing joints and is necessary for the formation of some joints. In contrast, recent data has shown that antagonism of the BMP family is crucial for joint formation. Here, we review mechanisms of how BMP signalling may be antagonised/modified. We also describe the expression of Bmp-2 and Bmp-4 together with two BMP antagonists, chordin and noggin, during chick joint development. Finally, we discuss possible mechanisms of how a joint forms and the evidence that the joint is a ’signalling centre’ that may coordinate the development of adjacent skeletal structures.
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    A comparison of the fine structure of thoracic and abdominal interganglionic connectives in the newly hatched and adult stick insect, Carausius morosus Br. (1973)
    Springer
    Cell & tissue research 145 (1973), S. 299-309 
    Details
    ISSN: 1432-0878
    Keywords: Nervous system ; Stick insect ; Blood-brain barrier ; Development ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The fat body sheath which surrounds the ventral nerve cord of the adult stick insect, Carausius morosus, is absent in the hatchling. Since the haemolymph sodium ion compositions of the two stages are similar, it is suggested that the fat body sheath is not a site of a blood-brain barrier dealing with extra-axonal regulation of sodium. Similarities of ultrastructure in connectives of adults and hatchlings are demonstrated, and tight junctions are shown to occur in perineural cells of both stages. The possible significance of this to the blood-brain barrier of this insect is discussed.
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    Untersuchungen über die Ultrastruktur der Gonaden von Chironomus (Dipt.) (1970)
    Springer
    Cell & tissue research 106 (1970), S. 348-370 
    Details
    ISSN: 1432-0878
    Keywords: Ovary ; Insect ; Chironomus ; Development ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Im Ovar von Chironomus sind in Phase 1 des 4. Larvenstadiums polygonal abgeflachte „Innenzellen“ von kleineren „Außenzellen“ umgeben, die Bakteroide und Phagosomen enthalten; zwischen den Innenzellen liegen unregelmäßige Zelltrümmer („keimbahnbegleitende Substanzen“). Zu Beginn der Ovariolenbildung werden in Phase 3 durch Spalträume zwei Schichten der Außenzellen voneinander getrennt, von denen die innere (Follikel- und Eikanalepithel) regelmäßige Buchten bildet. In diese Buchten wandern von innen Zellpaare ein, die an „synaptischen Komplexen“ bzw. multiplen Chromatinstrukturen als Ei- und Nährzellen kenntlich sind. Zwischen beiden Zellen sind „Fusome“ häufig, die später in eigentümlicher Weise geschlossen werden. Zwischen den Eikanalzellen entsteht in Phase 5 durch Spaltbildung der Eikanal; in Phase 7 sind die Eikanalzellen auffallend glykogenreich. Kurz vor der Vitellogenese treten im Bereich der Oocyte Membransysteme und „annulated lamellae“ auf; akzessorische Kerne werden als Ausstülpungen des Oocytenkernes gebildet und später abgeschnürt. In Phase 9 sind an der Peripherie der Eizelle Mikrovillisäume und Pinocytosebläschen sichtbar. Die distalen Zellen der Ovariole haben Eioder Nährzellcharakter, sind aber bei Ch. melanotus nicht von Follikelzellen umgeben und werden beim weiteren Ovariolenwachstum reduziert. Trotz extrem geringer Nährzellzahl der Follikel scheint das Chironomus-Ovar funktionell nicht von anderen polytroph meroistischen Insektenovarien unterschieden.
    Notes: Summary In the ovary of Chironomus during phase 1 of the fourth larval instar, polygonally flattened “inner cells” are surrounded by smaller “outer cells” which contain bacteroids and phagosomes. Irregular cell remnants (“germ line accompanying substances”) lie among the inner cells. At the beginning of ovariole formation in phase 3, two layers of outer cells are separated by the formation of fissures. The inner layer of these cells (follicle- and egg-passage epithelium) forms regular invaginations. Cell pairs, identified as oocytes and nurse cells by “synaptic complexes” or multiple chromatin structures, wander from inside into the invaginations. Frequently between the two cells are fusomes, which later close in a characteristic manner. During phase 5, an egg passage is formed as a fissure among the egg-passage cells. During phase 7, the egg passage cells are conspicuously full of glycogen. Shortly before vitellogenesis membrane systems and annulated lamellae appear in the region of the oocyte. Accessory nuclei are formed by a “tieing-off” of projections of the the oocyte nucleus. During phase 9, microvilli and pinocytotic vesicles can be seen at the periphery of the oocyte. The distal cells of the ovariole are of oocyte or nurse cell nature, but in Ch. melanotus they are not surrounded by follicle cells and are reduced during further ovariole growth. In spite of the extremely small number of nurse cells in the follicle, the Chironomus ovary apparently does not differ functionally from other polytrophic meroistic insect ovaries.
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    Studies on the effect of denervation in developing muscle (1973)
    Springer
    Cell & tissue research 147 (1973), S. 75-85 
    Details
    ISSN: 1432-0878
    Keywords: Skeletal muscle ; Denervation ; Development ; Fiber types, myofibrils ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Ultrastructural diversification of muscle fibers, with regard particularly to myofibrillar changes, has been investigated in the fast-twitch extensor digitorum longus (EDL) and the slow-twitch soleus muscles of the rat during fetal and postnatal development in the presence and in the absence of motor innervation. The band pattern and the shape of the myofibrils were uniform in fetal and neonatal muscle fibers and underwent differential changes during the first weeks after birth, concomitantly with fiber type specialization. The most evident variations in myofibrillar structure arising in this period concern the thickness of the Z band and the arrangement of the myofibrils. Myofibril formation was at first not impaired by denervation of rat muscles performed in utero and, although focal disintegration of myofibrils and detachment and loss of orientation of filaments became apparent by one week, atrophic muscle fibers with well-organized myofibrils could be seen as late as 2 months after birth. However, denervated muscle fibers of EDL and soleus did not display any significant and consistent difference in myofibrillar band pattern and shape. No variation in mitochondrial content and sarcoplasmic reticulum development was likewise seen in muscle fibers of EDL and soleus after fetal denervation. The findings emphasize the importance of neuromuscular interactions in muscle differentiation.
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    Structure and development of the tracheal organ in the mesothoracic leg of the cricket Teleogryllus commodus (Walker) (1974)
    Springer
    Cell & tissue research 147 (1974), S. 325-334 
    Details
    ISSN: 1432-0878
    Keywords: Chordotonal (Tracheal) organ ; Teleogryllus commodus (Orthoptera) ; Hearing ; Development ; Structure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The tracheal organ of the mesothoracic tibia of Teleogryllus is located in a corresponding position to the tympanal organ of the prothoracic tibia. The mesothoracic organ contains an average of only 12 scolopidia, the location of which corresponds to that of the proximal group A and proximal main group in the prothoracid tympanal organ. There are no scolopidia corresponding to the distal group of the tympanal organ. The variability in number of scolopidia is much greater in the mesothoracic organ than in the prothoracic organ. The adult tracheal system of the mesothoracic leg resembles the early nymphal tracheal system in both pro- and meso-thoracic legs. The development of the tracheal organ is usually complete by the sixth instar. The mesothoracic tracheal organ of the adult is broadly equivalent to the prothoracic tympanal organ of a fifth instar animal.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307468
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  • 97
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    Ultrastructure of synaptosomes from one-day old and adult rat brain stem (1974)
    Springer
    Cell & tissue research 148 (1974), S. 535-550 
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    ISSN: 1432-0878
    Keywords: Synaptosomes ; Rat brain stem ; Synaptogenesis ; Development ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure and protein content of the five subfractions of the crude mitochondrial fraction from the brain stem of the 1-day old and adult rat was examined. The morphological composition of the subfractions after fixation in glutaraldehyde and osmiumtetroxide in the adult rat brain stem resembled that previously reported for the whole brain; synaptosomes sedimented in a sucrose gradient in subfractions C and D. In the 1-day old rat, mature synaptosomes were found in subfractions A, B, C and D; E contained mainly free mitochondria. 80–95% of the processes in the adult and 10–30% in the 1-day old rat contained synaptic vesicles which were of four types: (1) small agranular vesicles (2) large dense core vesicles (3) large agranular vesicles (4) coated vesicles. Pre- and postsynaptic membrane thickenings were demonstrated in many nerve-ending particles. In the subfractions of the 1-day old rat the protein content was one half and the distribution resembled that in the adult. Evidently nerve endings develop faster in the brain stem than in cortical areas; a serotoninor adrenergic origin of the early synaptosomes is suggested.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00221937
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  • 98
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    Development of muscle spindles deprived of fusimotor innervation (1973)
    Springer
    Cell & tissue research 144 (1973), S. 435-452 
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    ISSN: 1432-0878
    Keywords: Muscle spindles ; Intrafusal fibre types ; Development ; Deefferentation ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Muscle spindles of limb muscles were deefferented in neonatal rats by sectioning ventral roots or by removal of the lumbosacral spinal cord. Ten to 56 days after the operation, muscle spindles were examined in the medial gastrocnemius, extensor digitorum longus and soleus muscles. The differentiation of muscle spindles was not affected by deefferentation. The number of spindles in the investigated muscles was not reduced. Intrafusal fibres increased in number from two at birth to four per spindle on the average, as in normal muscles. The characteristic ultrastructural distinctions of nuclear bag and nuclear chain fibres developed as under normal conditions. However, intrafusal fibres atrophied slowly after fusimotor denervation, their polar zones becoming reduced in diameter by about 25% in comparison with control fibre diameters. Spindle capsules, on the other hand, increased in size and attained diameters comparable with normal spindles, appearing even somewhat distended. As intrafusal fibres degenerate after complete denervation at birth (Zelená, 1957), but differentiate in the absence of fusimotor innervation, it can be concluded that sensory nerve terminals induce and support their development. It is assumed that the morphogenetic influence of sensory terminals is mediated by release and uptake of a trophic substance at the synaptic junction. The occurrence of light and dense core vesicles in the sensory terminals and of coated invaginations and vesicles at both the axonal and plasma membrane speak in favour of such a possibility.
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    URL: http://dx.doi.org/10.1007/BF00307586
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  • 99
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    Acinar ultrastructure of the submandibular gland of Mus musculus during embryonic development (1974)
    Springer
    Cell & tissue research 150 (1974), S. 425-442 
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    ISSN: 1432-0878
    Keywords: Glandula submandibularis (Mouse) ; Acinar ultrastructure ; Cell types ; Development ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The development of the submandibular gland of the mouse was studied by means of electron microscopy, from the 14th day of gestation up to birth. In the first two days the acini are solid and their cells contain polyribosomes and a few lamellae of endoplasmic reticulum. Beginning with the 16th day secretory granules appear and rapidly fill an increasing number of cells. The different electron density of the granules makes it possible to distinguish 1. two types of granules, dense and pale, and sometimes intermediate ones, 2. “polymorphic” granules. The latter consists of electron dense and electron pale parts combined in different configurations. The possible significance of the various types of granules is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00225967
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  • 100
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    Studies on the juxtaglomerular apparatus (1974)
    Springer
    Cell & tissue research 151 (1974), S. 433-456 
    Details
    ISSN: 1432-0878
    Keywords: Juxtaglomerular apparatus (Rat) ; Development ; Renin ; Pools ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Zusammenfassung Die perinatale Entwicklung des juxtaglomerulären Apparates, insbesondere das Verhalten der epitheloiden Zellen wurde an 18 trächtigen Ratten and 65 Embryonen bzw. Jungtieren elektronenmikroskopisch untersucht. Parallel dazu wurde die Reninkonzentration bzw. der Reningehalt bestimmt. Spezifische Sekretgranula finden sich erstmals am 18. Tag der Fetalzeit, und zwar in den epitheloiden Zellen des juxtaglomerulären Apparates juxtamedullärer Nephrone. Die Granula machen einen Struktur- und wahrscheinlich auch Funktionswandel durch, der eine Einteilung in drei verschiedene Granulatypen nahelegt. Diesen drei Typen spezifischer (reninhaltiger) Sekretgranula werden die Lysosomen als unspezifische Einschlüsse der epitheloiden Zellen gegenübergestellt. Im einzelnen kommt es während der perinatalen Phase in den fetalen Epitheloidzellen zuerst, d.h. pränatal, zu einer Hypertrophie des rauhwandigen endoplasmatischen Retikulums und des Golgi-Apparates. Hierin sehen die Autoren einen Hinweis auf eine erhöhte Proteinsynthese mit entsprechender Zunahme der Transport- und Konzentrationsvorgänge. Nach der Geburt findet sich dann auch eine deutliche Zunahme der Sekretgranula vom Typ III, die als reife Reningranula (mature granules) angesehen werden. Die bisher noch nicht beschriebenen kleinen Sekretgranula vom Typ I dagegen kommen in weniger entwickelten Epitheloidzellen häufiger vor. Bestimmungen der Reninverteilung zwischen Zytoplasma und den durch Differential-zentrifugation gewonnenen Granula-Fraktionen ergeben auffallende Parallelen zu den morphologischen Befunden. Diese sprechen im allgemeinen dafür, daß “freies Renin” im Ergasto-plasma und “gebundenes Renin” in den Sekretgranula vorliegt. Mit zunehmendem Alter scheint es außerdem — parallel zur relativen Abnahme des Ergastoplasmas und Zunahme der reifen Reningranula — zu einer Verminderung des extragranulären zugunsten des granulär gebundenen Renins zu kommen.
    Notes: Summary The perinatal development of the Juxtaglomerular apparatus (JGA), with particular reference to the epithelioid cells, was studied. Electron microscopic studies were carried out on 18 gravid rats and 65 embryos with corresponding determinations of renin concentration and the renin contents by bio-assay. Specific secretory granules of the epithelioid cells of the JGA of juxtamedullary nephrons are first observed on the 18th day of gestation. The classification into three types of secretory granules suggests that they undergo a structural and probably also a functional transformation. All three types of secretory granule may be regarded as specific, renin-containing, cytoplasmic organelles. During the last days of gestation, the rough-surfaced endoplasmic reticulum and the Golgi apparatus become hypertrophied in epithelioid cells, indicating an increased protein synthesis as well as transport and concentration processes. After birth, there is a definite increase in the number of Type III secretory granules-which may be regarded as mature granules. The small Type I secretory granulesnot previously described-are found more frequently in the immature epithelioid cells. Determinations of the renin distribution in the plasma and in fractions of cytoplasmic constituents (total homogenate, secretory granules) prepared by differential centrifugation showed striking congruence with the morphological findings. It is generally assumed that “soluble renin” occurs in the endoplasmic reticulum and “bound renin” in secretory granules. A shift from extragranular to granular (bound) renin, occurs with increasing age, having its morphological equivalent in the relative decrease of the endoplasmic reticulum and increase in mature secretory granules.
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    URL: http://dx.doi.org/10.1007/BF00222990
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