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  • 04. Solid Earth::04.06. Seismology::04.06.05. Historical seismology
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  • 1
    Publikationsdatum: 2017-04-04
    Beschreibung: Mostly based on traditional catalogues, without further research, several modern parametric catalogues are nevertheless straightforward, without question marks, and easily misleading (chronology, epicentre, epicentral intensity, not to speak of magnitude). The example of an Ionian time-window (1658-1664), with several major events, shows that the historical seismicity of the Ionian Islands, often thought to be well-known, actually needs a more or less drastic revision. A wealth of sources was collected, mostly from the Archives of the Republic of Venice, then ruling the main three islands of the Ionian Archipelago; it was ascertained that there are no important chronological gaps in the surviving documentation. Similarly outstanding, and in fact at the basis of a more balanced and precise view of one of the events in this time-window, are the souvenirs of Christoff von Degenfeld, a German nobleman at the service of the Republic of Venice. His manuscript, discovered at the library of Karlsruhe (Germany) in 1992, has been consulted again in the original, on the occasion of the preparation of this paper. Some question marks remain on the distributions of macroseismic effects of the earthquakes within this time-window, and this is due to the lack of information concerning the mainland. For this reason this study does not propose epicentres and, of course, magnitudes. An unusually long documentary appendix is provided, with the hope that it might contribute in discouraging authors of parametric earthquake catalogues from hasty exploitation and interpretation of often unreliable current catalogues.
    Beschreibung: Published
    Beschreibung: 43-91
    Beschreibung: 3.10. Sismologia storica e archeosismologia
    Beschreibung: open
    Schlagwort(e): Historical seismology ; Ionian Islands 1658-1664 ; 04. Solid Earth::04.06. Seismology::04.06.05. Historical seismology
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: book chapter
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  • 2
    Publikationsdatum: 2017-04-04
    Beschreibung: Most of the ancient town of Tindari (NE, Sicily) was settled on a plateau the most surficial layer of which was made of unconsolidated material. Ongoing excavations at the archaeological site at Tindari uncovered a large portion of the decumanus which suffered deformations preliminarily assigned to coseismic effects. An analysis of the local dynamic response through the simulation of strong seismic shaking to the bedrock and modelling of spectral ratios of the bedrock-soft soil was carried out to verify the susceptibility of superficial terrains of the promontory to coseismic deformations. To perform this simulation the finite element method (FEM) was used. Four accelerometric recordings of three earthquakes of medium-high magnitude, recorded on rocky sites, were chosen to simulate the seismic shaking, using a constitutive law for the materials composing the promontory layers both of linear-elastic type and of elastoplastic type. The analysis of the linear-elastic field allowed the definition of the frequencies for which the spectral ratios of the accelerations recorded the highest amplifications; in particular the frequency range 31.5–37.2 Hz can be combined with deformation of the paved floor of the decumanus. The analysis in the elastoplastic field highlighted the zones of promontory more susceptible to suffer plasticization process. The results show that the topmost layer of the decumanus is the most susceptible to suffer plasticization. Therefore, the performed analysis lends greater support to the hypothesis that the deformations were produced by seismic shaking.
    Beschreibung: Published
    Beschreibung: 213-222
    Beschreibung: 3.10. Sismologia storica e archeosismologia
    Beschreibung: JCR Journal
    Beschreibung: reserved
    Schlagwort(e): Fourier analysis ; Elasticity and anelasticity ; Earthquake ground motions ; Site effects ; Computational seismology ; 04. Solid Earth::04.06. Seismology::04.06.99. General or miscellaneous ; 04. Solid Earth::04.06. Seismology::04.06.05. Historical seismology
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
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  • 3
    Publikationsdatum: 2017-04-04
    Beschreibung: Jean Vogt was born in 1929 in Strasbourg (France), where he attended primary and secondary school. At the University of Strasbourg, he graduated in Geography, and majored in Geomorphology. His professor was the geographer Jean Tricart, who taught him the importance of both geological field work and archive investigation. In 1955 he joined the French West-Africa Geological Service and later the French Bureau for Geology and Mines (BRGM). Along the following 20 years he lived as a “geological” globetrotter in a number of countries, dispensing his time between the field and the archives. In these years, he was concerned mainly with mining geology, geomorphology, superficial deposits, and landslides. This unique experience led him in 1975 to the responsibility of the “Seismo-Tectonic Project”, the BRGM project in relation with the French nuclear power programme. From 1975 to 1984, he gave a substantial impulse to the study of French historical earthquakes, and since then he visited almost every public archive in France, and several major archives and libraries in Europe and abroad. He took care at the same time of the follow-up of macroseismic studies of present-day earthquakes. After he retired in 1984, he continued on a personal basis his investigations of historical earthquakes, in Europe, the Middle East, North Africa, and the Caribbean area. Alongside and for about 50 years, Jean Vogt investigated uninterruptedly the agrarian history of Northeastern France and Southwestern Germany. He published in scientific journals and in local learned societies bulletins more than 500 notes and articles devoted to a variety of subjects, such as soil erosion, agriculture, cattle trade, and social conflicts. Jean Vogt died on 5 June 2005 in Strasbourg. His scientific legacy consists of a wealth of published papers, manuscripts, documentation related to history and seismology, awaiting to be further exploited, as he would have done.
    Beschreibung: Published
    Beschreibung: 3-16
    Beschreibung: 3.10. Sismologia storica e archeosismologia
    Beschreibung: reserved
    Schlagwort(e): Jean Vogt ; biography ; historical seismology ; 04. Solid Earth::04.06. Seismology::04.06.05. Historical seismology
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: book chapter
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  • 4
    Publikationsdatum: 2017-04-04
    Beschreibung: The recent seismological literature recorded three strong earthquakes in Algeria, Libya and Tunisia between 1656 and 1694 AD. The historical evidence for these derives from European sources only (gazettes, journalistic pamphlets, missionary literature). Considering the kind of sources involved, their likely biases and the geographical distances that divided their places of production from the places that they spoke about, it is possible that some of these accounts could be less than reliable, and therefore have little use as materials from which to assess earthquake parameters. To answer these doubts, we have retrieved, cross-checked and critically analysed the original historical sources quoted in previous compilations and studies.
    Beschreibung: Published
    Beschreibung: 163-184
    Beschreibung: 5.1. TTC - Banche dati e metodi macrosismici
    Beschreibung: reserved
    Schlagwort(e): Historical Seismicity ; North Africa Earthquakes ; 04. Solid Earth::04.06. Seismology::04.06.05. Historical seismology
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: book chapter
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  • 5
    Publikationsdatum: 2017-04-04
    Beschreibung: While the seismicity of the Southern Alps is high in the Eastern sector, corresponding to the Veneto and Friuli regions, it decreases towards West up to the Adda River. In the sector between the Lessini Mts. and Eastern Friuli the damaging earthquakes are clustered in a well defined seismic belt, where seismogenic sources responsible for earthquakes with Mw 6 have been defined in recent works. In contrast, the knowledge of the Southalpine sector West of this area is sparser; the area experienced some earthquakes with Mw〉5.5 and varied events with 4.8≤Mw≤5.5 the distribution of which is, apparently, random. For the area roughly defined by the basins of the Adda River to the West and the middle Adige River to the East, this paper reappraises the background knowledge of the earthquakes occurred before 1700. The investigation and the results are presented according to two successive periods, up to 1995 and from 1995 on. In the research performed up to 1995, the most important achievements concerned two different aspects: i) the assessement of several “fake quakes”, some of which were the object of paradigmatic case-histories; ii) the resizing and relocation of several, presumed damaging earthquakes. Though this round of investigation changed significantly the picture of the seismicity with respect to the Seventies, the research continued. For the period from 1995 on, the discussion focuses on the reliability of the available information; material that received little or no consideration before, new historical findings and comments to the seismological interpretation as in the most recent literature are also presented. This part includes also the discussion of archaeoseismological evidence of damage related to past earthquakes.
    Beschreibung: Published
    Beschreibung: 93-129
    Beschreibung: 3.10. Sismologia storica e archeosismologia
    Beschreibung: reserved
    Schlagwort(e): historical seismology ; Adda and Middle Adige River Basins ; Southern Alps ; archaeoseismology ; 04. Solid Earth::04.06. Seismology::04.06.05. Historical seismology
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: book chapter
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  • 6
    Publikationsdatum: 2017-04-03
    Beschreibung: The Valtiberina region (central Italy) has a seismic record going back to the Middle Ages and including five Io 〉 VIII MCS earthquakes, the earliest of which (1352, 1389, 1458). though recently and extensively studied, remain rather poorly known. This makes it all the more important to ensure that the later ones (1789, 1917) are as throughly studied as possible. The 1789 earthquake is listed by the current Italian catalogue (CPTI Working Group 2004) with Io VIII-IX MCS and Mm 5.8. These parameters were assessed from a database of 28 macroseismic intensity data points (Castelli et al. 1996), which is less than plentiful for a late 18th century earthquake. An analysis of the historical context of the 1789 earthquake and its influence on the production of contemporary accounts evidences a few research paths that previous studies either did not or could not take. Following them, the macroseismic database of the 1789 earthquake can be noticeably improved, providing the catalogue compiler with a mean to check the reliability of its current parameters.
    Beschreibung: Published
    Beschreibung: 249-260
    Beschreibung: 5.1. TTC - Banche dati e metodi macrosismici
    Beschreibung: open
    Schlagwort(e): Historical Seismicity ; 1789 Valtiberina Earthquake ; 04. Solid Earth::04.06. Seismology::04.06.05. Historical seismology
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: book chapter
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  • 7
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    Publikationsdatum: 2020-02-12
    Schlagwort(e): 550 - Earth sciences
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  • 8
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    Publikationsdatum: 2020-02-12
    Schlagwort(e): 550 - Earth sciences
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  • 9
    Publikationsdatum: 2020-11-30
    Beschreibung: This special issue of Journal of Seismology, dedicated to Archaeoseismology, shows the current trends of research in this young branch of seismology. The beginning of modern archaeoseismological practice during the 1980’s and the investigations made during the 1990’s were in many cases conditioned by the lack of cooperation among the spcialists from different scientific diciplines. Numerous publications resulted from the work of archaeologists, in some cases in collaboration with experts in earthquake-geology or seismology, but rarely such work included a complete and multidisciplinary approach and in situ analysis of the evidence. In many cases, archaeoseismological studies were limited to the detection of traces of past earthquakes in archaeological remains without a seismological perspective aiming to derive quantitative parameters necessary to fully describe a past earthquake (magnitude, etc.). In other cases, such investigations were limited to the analysis of archaeological reports of excavations made years or decades earlier. A step towards the quantification of seismological aspects was represented by the “territorial” approach, trying to reconstruct a picture of an earthquake by detecting its signs over a wider region. This procedure was, however, limited by the scarcity and reliability of published or unpublished archaeological material, usually adopted without a critical review.
    Beschreibung: Published
    Beschreibung: 393–394
    Beschreibung: 2TM. Divulgazione Scientifica
    Beschreibung: JCR Journal
    Beschreibung: open
    Schlagwort(e): Archaeoseismology, ; 04. Solid Earth::04.06. Seismology::04.06.05. Historical seismology
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
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  • 10
    Publikationsdatum: 2012-02-03
    Beschreibung: Archaeoseismic research contributes important data on past earthquakes. A limitation of the usefulness of archaeoseismology is due to the lack of continuous discussion about the methodology. The methodological issues are particularly important because archaeoseismological investigations of past earthquakes make use of a large variety of methods. Typical in situ investigations include: (1) reconstruction of the local archaeological stratigraphy aimed at defining the correct position and chronology of a destruction layer, presumably related to an earthquake; (2) analysis of the deformations potentially due to seismic shaking or secondary earthquake effects, detectable on walls; (3) analysis of the depositional characteristics of the collapsed material; (4) investigations of the local geology and geomorphology to define possible natural cause(s) of the destruction; (5) investigations of the local factors affecting the ground motion amplifications; and (6) estimation of the dynamic excitation, which affected the site under investigation. Subsequently, a ‘territorial’ approach testing evidence of synchronous destruction in a certain region may delineate the extent of the area struck by the earthquake. The most reliable results of an archaeoseismological investigation are obtained by application of modern geoarchaeological practice (archaeological stratigraphy plus geological–geomorphological data), with the addition of a geophysicalengineering quantitative approach and (if available) historical information. This gives a basic dataset necessary to perform quantitative analyses which, in turn, corroborate the archaeoseismic hypothesis. Since archaeoseismological investigations can reveal the possible natural causes of destruction at a site, they contribute to the wider field of environmental archaeology, that seeks to define the history of the relationship between humans and the environment. Finally, through the improvement of the knowledge on the past seismicity, these studies can contribute to the regional estimation of seismic hazard.
    Beschreibung: Published
    Beschreibung: 395-414
    Beschreibung: JCR Journal
    Beschreibung: reserved
    Schlagwort(e): archaeoseismology ; historical seismology ; geoarchaeology ; environmental archaeology ; natural catastrophes ; 04. Solid Earth::04.04. Geology::04.04.01. Earthquake geology and paleoseismology ; 04. Solid Earth::04.06. Seismology::04.06.05. Historical seismology
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
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  • 11
    Publikationsdatum: 2017-04-04
    Beschreibung: The southernmost sector of the Italian peninsula is crossed by an almost continuous seismogenic belt capable of producing M ~ 7 earthquakes and extending from the Calabrian Arc, through the Messina Straits, as far as Southeastern Sicily. Though large earthquakes occurring in this region during the last Millennium are fairly well known from the historical point of view and seismic catalogues may be considered complete for destructive and badly damaging events (IX £ Io £ XI MCS), the knowledge and seismic completeness of moderate earthquakes can be improved by investigating other kinds of documentary sources not explored by the classical seismological tradition. In this paper, we present a case study explanatory of the problem, regarding the Ionian coast between the Messina Straits and Mount Etna volcano, an area of North-eastern Sicily lacking evidence of relevant seismic activity in historical times. Now, after a systematic analysis of the 18th century journalistic sources (gazettes), this gap can be partly filled by the rediscovery of a seismic sequence that took place in 1780. According to the available catalogues, the only event on record for this year is a minor shock (Io = VI MCS, Mw = 4.8) recorded in Messina on March 28, 1780. The newly discovered data allow to reinstate it as the mainshock (Io = VII–VIII MCS, Mw = 5.6) of a significant seismic period, which went on from March to June 1780, causing severe damage along the Ionian coast of North-eastern Sicily. The source responsible for this event appears located offshore, 40-km south of the previous determination, and is consistent with the Taormina Fault suggested by the geological literature, developing in the low seismic rate zone at the southernmost termination of the 1908 Messina earthquake fault.
    Beschreibung: Published
    Beschreibung: reserved
    Schlagwort(e): Historical seismology ; Macroseismic data ; MCS-EMS intensity scales ; 1780 Seismic sequence ; Seismotectonics ; NE Sicily ; 04. Solid Earth::04.06. Seismology::04.06.03. Earthquake source and dynamics ; 04. Solid Earth::04.06. Seismology::04.06.05. Historical seismology
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
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  • 12
    Publikationsdatum: 2017-04-03
    Beschreibung: The aim of this study is to shed light ontwo important destructive seismicsequences, about 20 years apart (1138–1139and 1156–1159), which hit the northernsector of the Dead Sea transform faultsystem (DSTFS), in the easternMediterranean region. Although some ofthese earthquakes were already known to thescholarly tradition, the interpretationsprovided until today have largely beenpartial and characterised by uncertaintiesand discrepancies among the variousauthors. Our study has developed throughresearch into the original Arabic, Syriac,Armenian and Latin texts and a criticalanalysis relating to a territory fragmentedby the presence of the Christian-Latinstates. This analysis has allowed us toshed light on the already existing, albeitoften uncertain, information and to add newelements of these two important series ofearthquake shocks to our knowledge base.As regards the first seismic sequence(October 1138–June 1139), apart from havingdefined the date with greater accuracy,eight new locations affected have beenidentified, unknown to previous studies.The shocks jolted a vast area withdestructive effects, including theterritory of Aleppo (modern Halab, Syria)and the western part of the region ofEdessa (modern Urfa, Turkey).The second seismic sequence (September1156–May 1159) was much longer anddevastating, and hit a huge area, includedbetween the present-day territories ofnorth-western Syria, northern Lebanon andthe region of Antioch (modern Antakya, insouthern Turkey). A detailed analysis ofthe primary sources has allowed toreconstruct the series of shocks withchronological detail of the effects,improving our previous knowledge. Lastly,the authors formulate an hypothesis as tothe possible seismogenic zones affected.
    Beschreibung: Published
    Beschreibung: 105-127
    Beschreibung: 3.10. Sismologia storica e archeosismologia
    Beschreibung: JCR Journal
    Beschreibung: reserved
    Schlagwort(e): Historical earthquakes ; seismic sequences ; Lebanon ; Syria ; 04. Solid Earth::04.06. Seismology::04.06.99. General or miscellaneous ; 04. Solid Earth::04.06. Seismology::04.06.05. Historical seismology
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: article
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  • 13
    Publikationsdatum: 2017-04-04
    Beschreibung: The recent seismological literature recorded three strong earthquakes in Algeria, Libya and Tunisia between 1656 and 1694 AD. The historical evidence for these derives from European sources only (gazettes, journalistic pamphlets, missionary literature). Considering the kind of sources involved, their likely biases and the geographical distances that divided their places of production from the places that they spoke about, it is possible that some of these accounts could be less than reliable, and therefore have little use as materials from which to assess earthquake parameters. To answer these doubts, we have retrieved, cross-checked and critically analysed the original historical sources quoted in previous compilations and studies.
    Beschreibung: Unpublished
    Beschreibung: 3.10. Sismologia storica e archeosismologia
    Beschreibung: open
    Schlagwort(e): Historical Seismology ; North Africa Earthquakes ; Early journalistic sources ; 04. Solid Earth::04.06. Seismology::04.06.05. Historical seismology
    Repository-Name: Istituto Nazionale di Geofisica e Vulcanologia (INGV)
    Materialart: book chapter
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  • 14
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    Publikationsdatum: 2020-02-12
    Schlagwort(e): 550 - Earth sciences
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  • 15
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    Publikationsdatum: 2020-02-12
    Schlagwort(e): 550 - Earth sciences
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  • 16
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    Publikationsdatum: 2020-02-12
    Schlagwort(e): 550 - Earth sciences
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  • 17
    Publikationsdatum: 2020-02-12
    Schlagwort(e): 550 - Earth sciences
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  • 18
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    Publikationsdatum: 2020-02-12
    Schlagwort(e): 550 - Earth sciences
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  • 19
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    Publikationsdatum: 2020-02-12
    Schlagwort(e): 550 - Earth sciences
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  • 20
    Publikationsdatum: 2020-02-12
    Beschreibung: In Südostalaska wird eine relative Meeresspiegelabsenkung von bis zu 4 cm/ a beobachtet. Da dieses Gebiet während der letzten Kaltperiode durch das Kordillerische Eisschild bedeckt war und noch heute von Vergletscherung geprägt ist, liegt die Vermutung nahe, daß die Meeresspiegeländerungen zumindest teilweise durch isostatische Ausgleichsvorgänge hervorgerufen werden. Um diese Hypothese zu prüfen, werden für dieses Gebiet eislastinduzierte Vertikalbewegungen und Geoidänderungen mit Hilfe viskoelastischer Erdmodelle berechnet. Insbesondere werden sechs siebenschichtige Erdmodelle verwendet, die unterschiedliche Viskostitätsprofile berücksichtigen und die Bandbreite der plausiblen Lithosphärenmächtigkeiten abdecken. Das globale Lastmodell ICE-3G wird erweitert und das regionale Lastmodell AL-1 entwickelt, welches die rezente Vergletscherung Südostalaskas berücksichtigt. Weiterhin werden die Ozeane als Last einbezogen. Ein Vergleich der berechneten mit den beobachteten Meeresspiegeländerungen zeigt, daß die Beobachtungen nur zu einen geringen Anteil von wenigen Millimetern pro Jahr durch Isostasie erklärt werden können.
    Schlagwort(e): 550 - Earth sciences
    Sprache: Deutsch
    Materialart: info:eu-repo/semantics/report
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  • 21
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    Deutsches GeoForschungsZentrum GFZ
    In:  Scientific Technical Report STR
    Publikationsdatum: 2021-02-25
    Beschreibung: Die im Norddeutschen Becken vorkommenden hochsalinaren, heißen Tiefenwässer besitzen ein beträchtliches Wärmepotential, das bisher in Neustadt-Glewe, Neubrandenburg und Waren zur Energiegewinnung genutzt wird. Das Ziel der vorliegenden Arbeit bestand zum einen darin, die Herkunft und die Genese dieser Solen und der in ihnen gelösten Stoffe zu rekonstruieren. Ein zweiter Schwerpunkt bestand in der Ermittlung geochemischer Parameter, die für einen problemlosen Langzeitbetrieb geothermischer Anlagen und somit für deren Wirtschaftlichkeit von Bedeutung sind. Die untersuchten Solen stammten aus Aquiferen, die durch fünf Bohrungen (Neubrandenburg, Waren, Rheinsberg, Neustadt-Glewe, Hamburg-Allermöhe) in Tiefen von 1250 bis 3250 m (54 bis l28°C) erschlossen wurden. Alle untersuchten Na-Cl-Solen wurden aus Sandsteinaquiferen des Keuper (Contorta!Postera-Schichten) gefördert, für die mit zunehmender Tiefe steigende TDS-Gehalte (134 bis 224 g/1) charakteristisch sind. Die Zusammensetzung der gelösten Feststoffe blieb über einen Zeitraum von 3 Jahren konstant. Die Br/CJ-Verhältnisse der Solen sind kleiner als in eingedampftem Meerwasser. Dies und 811B-Werte von +23.8 %o bis +36.3 %o indizieren die Herkunft des Salzgehaltes durch Auflösung/Laugung von Salzen. Das Gas-Wasser-Volumenverhältnis in Proben geothermisch genutzter Solen liegt bei max. 1:10. Die Gasphase wird von C02, N2 und CH4 dominiert, wobei der Gehalt an C02 und CH4 mit der Tiefe zunimmt. Als Spurengase treten He, Ar, H2 und weitere Kohlenwasserstoffe auf (jeweils 〈1 Vol.-%). Die Gasphase des Thermalwassers Neustadt-Giewe zeigte über einen Zeitraum von 2 Jahren keine Änderung in ihrer Zusammensetzung. Periodische Schwankungen in sehr kleinen Konzentrationsbereichen während einer mehrtägigen Gasmeßkampagne korrelieren mit den Erdgezeiten. Die N2-Ar-He-Verhältnisse zeigen, daß es sich um tiefzirkulierende Oberflächenwässer handelt. Untermauert wird diese Interpretation durch die sehr niedrigen 3HefHe-Verhältnisse (R!Ra:S;O.Ol), die keine Mantelheliumkomponente anzeigen, sowie die gegenüber Luft (295.5) leicht erhöhten 40 Ar/36 Ar-Verhältnisse von bis zu 367.5. He-Akkumulationsalter als scheinbare Verweildauer der Solen von 20 bis 50 Ma wurden berechnet. Das Tiefenwasser von NeustadtGiewe besaß gegenüber den anderen Lokationen erhöhte Kohlenwasserstoffgehalte. Die 813C1,2,3- Werte weisen auf thermogenetische Kohlenwasserstoffe und auf ein marines Ausgangsmaterial mit einer Reife, die einer Vitrinitreflektion von etwa 1.2 % entspricht, hin. Die Herkunft aus Corgreichen Zechstein-Sedimenten ist wahrscheinlich, organisches Material des wesentlich tiefer liegenden Karbons scheidet als Quelle aus. Die 8180- und öD-Werte der Wässer zeigen an, daß meteorisches Wasser bei der Genese eine bedeutende Rolle gespielt hat. Die ermittelte isatopische Zusammensetzung der meteorischen Komponente weicht von heutigen Niederschlagswässern ab und weist einen letztmaligen Kontakt mit der Atmosphäre zu einer Zeit deutlich wärmeren Klimas als das heutige nach. Die in einem Thermalwasser vorhandenen Gase können die technologische Eignung der Sole und den Betrieb einer geothermischen Anlage maßgeblich beinflussen (Scaling, Auftreten brennbarer Gase, Entlösung von Gasen). Bei Vorhandensein Corg-reicher Sedimente nimmt die Wahrscheinlichkeit des Auftretens brennbarer Gase in Wässern aus größerer Tiefe, die durch ihre höhere Temperatur wirtschaftlicher sind, zu. Um die Entgasung eines Tiefenwassers innerhalb einer Anlage zu verhindem und somit Scaling und unkontrolliertes Entgasen zu minimieren, wurde anhand Literaturdaten exemplarisch für den Thermalwasserkreislauf der Anlage Neustadt-Giewe (Sole mit den höchsten Gasgehalten) ein Mindestanlagendruck berechnet. Danach sind etwa 2 bar ausreichend, um ein Entgasen der Sole zu verhindern; Strömungseffekte erfordern jedoch eine Erhöhung des Anlagendrucks auf etwa 4 bar. Die zeitliche Konstanz in der Zusammensetzung der gelösten Feststoffe und der Gasphase über einen Zeitraum von 2 bzw. 3 Jahren zeugt von einer relativ großen räumlichen Homogenität der Aquifere, wodurch der Betrieb der Anlagen durch Änderungen in der chemischen Zusammensetzung der Solen kaum gefährdet zu sein scheint. Die Ergebnisse der vorliegenden Arbeit haben gezeigt, daß sowohl bei der hydrodynamischen Vorerkundung als auch während des Betriebes eines geothermischen Heizwerkes die genaue Kenntnis der Gasmengen und der Gaszusammensetzung von entscheidender Bedeutung ist.
    Beschreibung: Deep seated hydrothermal brines in the North German Basin have a considerable heat potential, which is currently used for geothermal energy recovery in Neustadt-Glewe, Neubrandenburg and Waren. One aim of this study was to reconstruct the origin of these brines and their dissolved substances. Another goalwas to determine geochemical parameters which are critically influence the long-term operation, and hence the econornic viability of geothermal plants. The brines come from aquifers which have been accessed by 5 boreholes (Neubrandenburg, Waren, Rheinsberg, Neustadt-Glewe, Hamburg-Allermöhe) in depths from 1250 to 3250 m (54 to 128 °C). All investigated Na-Cl-brines were produced from sandstone aquifers of the Keuper (Contorta!Postera layers). Typically, TDS-values (134 to 224 g/1) increase with depth. The compositions of the dissolved solids were constant during 3 years. The Br/Cl ratios of the brines are lower than evaporated sea water. This, and 811B-values of +23.8 %o up to +36.3 %o indicate dissolution/leaching of salt as the origin of the salt content. The gas-water volume ratio of samples from brines used for geothermal energy recovery are less than around 1:10. The gas phase is dorninated by C02, N2 and C~ with higher C02- and C~ contents with increasing depths. He, Ar, H2 and other hydrocarbons exist as traces ( 〈1 vol.% each). The gas phase of the thermal water from Neustadt-Giewe showed no changes in its composition during 2 years. Small · periodical variations in the concentrations obtained from gas monitaring over several days correlate with the earth tides. The N2-Ar-He ratios show that the waters are deep-circulating meteoric waters. This interpretation is supported by very low 3HefHe ratios (R!Ra:S;O.Ol), which show no mantle helium, and 40 ArP6 Ar ratios up to 367.5, which are slightly enhanced compared to air (295.5). He accumulation ages, taken as the apparent residence time of the brines, were calculated to be 20 to 50 Ma. Compared to the other locations the formation water from Neustadt-Giewe contained enhanced hydrocarbon contents. The 813C1,2,3 values point to thermogenic hydrocarbons and to a marine source rock with a maturity corresponding to about 1.2 % vitrinite reflectivity. An origin from Corg-rich Zechstein sediments seems probable, organic material from significantly deeper-seated Carboniferous formations can be ruled out. The 8180 and ÖD values of the waters confirm that meteoric water played an important role in the brine genesis. The determined isotopic composition of the meteoric component deviates from recent precipitation and indicates a last contact with the atmosphere at a time when climate conditions were significantly warmer than today. Gases dissolved in thermal waters can significantly affect both the technological suitability of a brine and the operation of a geothermal heat plant (scaling, degassing, occurence of flammable gases). If Corg-rich sedimentary rocks occur, the probability of the occurence of flammable gases increases in waters from larger depths, which are more economic due to their higher temperature. The pressure needed to prevent degassing inside a plant and thus to rninirnize scaling and uncontrolled degassing was calculated for the example of the thermal water cycle at Neustadt-Giewe (brine with the highest gas content). About 2 bars are sufficient to prevent degassing; however, flow effects require increasing the pressure up to about 4 bars. The constant composition of both the dissolved solids and gases over a period of 2 to 3 years is a sign of a relatively large spatial uniformity of the aquifers. Therefore the continuous operation of the plants seems unlikely to be endangered by a change in the chernical composition of the brines. The results of this study have shown that during the hydrodynarnic reconnaissance as weil as during the operation of a geothermal heat plant, precise knowlegde of the gas concentration is of essential importance.
    Schlagwort(e): 550 - Earth sciences
    Sprache: Deutsch
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  • 22
    Publikationsdatum: 2022-11-15
    Schlagwort(e): 550 - Earth sciences
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  • 23
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    In:  Scientific Technical Report STR
    Publikationsdatum: 2022-11-24
    Schlagwort(e): 550 - Earth sciences
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  • 24
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    In:  Scientific Technical Report STR
    Publikationsdatum: 2022-11-24
    Schlagwort(e): 550 - Earth sciences
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  • 25
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    In:  Scientific Technical Report STR
    Publikationsdatum: 2022-11-24
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  • 26
    Publikationsdatum: 2022-11-24
    Schlagwort(e): 550 - Earth sciences
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  • 27
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    In:  Scientific Technical Report STR
    Publikationsdatum: 2022-11-24
    Schlagwort(e): 550 - Earth sciences
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  • 28
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    Deutsches GeoForschungsZentrum GFZ
    In:  Scientific Technical Report STR
    Publikationsdatum: 2022-11-24
    Schlagwort(e): 550 - Earth sciences
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  • 29
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    Deutsches GeoForschungsZentrum GFZ
    In:  Scientific Technical Report STR
    Publikationsdatum: 2022-11-24
    Beschreibung: Receiver function analysis is routinely used to isolates P-to-S converted waves from a complex of earthquake recordings s as to explore crustal and upper mantle structures and to infer possible geodynamic processes within the Earth. In the last several years the number of deployments of portable seismic arrays has been greatly increased. The conventional receiver function method, which stacks receiver functions at a single station, is not suitable for such a large amount of data. In this thesis modifications of the receiver function method have been made. Techniques of reflection seismology have been introduced into the receiver function analysis. Modified receiver function method has been successfully applied to the seismological data acquired in Tibet and the Central Andes. In these two Earth"s largest and highest plateaus, data of many available seismic broadband and short-period experiments have been collected. In Tibet, data of the INDEPTH II and GEDEPTH I experiments in southern Tibet and the PASSCAL 91/92 experiment across the central Tibetan Plateau have been combined. A Total number of about 50 stations were distributed roughly in a NNE directed profile. More than 900 receiver functions have been obtained. In the Central Andes, more than 200 stations have been deployed within the experiments of PISCO, CINCA, ANCORP, PUNA and KDS of the project of the SFB 267, and the BANJO and SEDA broadband arrays of the PASSCAL experiments. More than 640 teleseismic receiver functions have been obtained. Results are summarized in the following. (1) Crustal thicknesses under the two plateaus are reliably determined by Teleseismic receiver functions. P-to-S converted waves at the Moho are clearly seen under the Tibetan plateau and under Central Andean plateau. In southern Tibet the Moho is 75-80 km deep. In northern Tibet it becomes shallower to a depth of 55-60 km. In the Central Andes, the continental Moho is 65-70 km deep beneath the Andean Plateau (it appears to be 15 km shallower beneath Puna than beneath Altiplano). The Moho abruptly reduces its depth beneath the eastern edge of the Eastern Cordillera (65-64.5°W) and remains 45-50 km depth in the Sub Andes. Further east there is another abrupt reduction of Moho depth between the Sub Andes and the Chaco Plain. The Moho is 30-35 km beneath the Chaco Plain. (2) Evidence of crustal-scale underthrusting is found in Tibet as well as in the Andes. The INDEPTH data clearly show an intra-crustal phase at a depth of 50-60 km in the southern Tibet. This conversion boundary is probably the evidence of the underthrust Indian crust. In the Andean data a more than 300 km west-dipping intra-crustal converter evidently marks the boundary of the underthrust Brazilian shield crust. This boundary exists across the entire Altiplano and Puna plateau from 20 km depth below the Eastern Cordillera to 40 km depth below the Western Cordillera and the Precordillera. In both plateaus, most of the thickened crust, if not all, can be attributed to the crustal-scale underthrusting. (3) Plate boundaries are found to a depth of about 250 km between the Indian and the Asian lithospheric mantle and to a depth of about 120 km between the Nazca plate and the South American plate. However, the nature of these boundaries is different. In the Central Andes, the plate boundary is interpreted as the oceanic Moho of the Nazca plate, above which a 10 km layer of oceanic crust with lower seismic velocity suggests that the gabbroic rocks do not completely transform to eclogite until a depth of 120 km. Most of the intermediate depth seismicity stops at the same depth, suggesting a relation with phase transformation. In Tibet the observed plate boundary of the two lithosperic mantles probably reflects the temperature difference between the two plates. The cold Indian mantle is subducted under the warm Asian mantle. The Temperature difference can be as high as 500.700 ° resulting in large seismic velocity contrast. (4) Interesting variations have been found in the upper mantle discontinuities which are related to the plate collision and subduction processes. In Tibet, the 410 km discontinuity is clearly seen in its globally average depth in the south, and is disturbed and becomes complicated in the north. The 660 km discontinuity is continuously displayed throughout the Tibetan profile. Similarly, in the Central Andes, the 410 km discontinuity is not imaged coherently, which is obviously attributed to the subduction complexity of the phase transformation of the mantle rocks. It is interesting to see that the 660 km discontinuity is depressed by about 30-40 km in the region of the cold Nazca slab, which corresponds to a temperature reduction of 300-600° within the slab.
    Schlagwort(e): 550 - Earth sciences
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  • 30
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    Publikationsdatum: 2022-11-24
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  • 31
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    Publikationsdatum: 2022-11-24
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  • 32
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  • 33
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  • 34
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  • 35
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  • 36
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  • 37
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  • 38
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  • 39
    Digitale Medien
    Digitale Medien
    Springer
    Entomologia experimentalis et applicata 95 (2000), S. 97-103 
    ISSN: 1570-7458
    Schlagwort(e): butterfly ; Formicidae ; food ; growth ; mutualism ; myrmecophily ; parasitism
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Caterpillars of the lycaenid butterfly Maculinea rebeli Hirschke (Lepidoptera: Lycaenidae) live for 11–23 months as social parasites in Myrmica (Hymenoptera: Formicidae) red ant nests, a trait that is believed to have evolved from mutualistic myrmecophilous ancestry. Although Maculinea rebeli caterpillars harm Myrmica larvae, they simultaneously produce copious secretions which the adult worker ants imbibe, perhaps representing a vestige of the ancestral mutualism. We report the results of laboratory experiments designed to test alternative hypotheses: (i) Maculinea rebeli caterpillars provide a beneficial source of sugar in return for being tended by Myrmicaworkers; (ii) Maculinea rebeli harms its host by stressing the workers by competing for available sugar. Comparisons were made of Myrmica worker fitness after 90–450 days under all possible combinations of three experimental treatments: ± M. rebeli caterpillars, ± sucrose and ± ant brood. Caterpillars always reduced the survival of both ant workers and their larvae, even when sugar was not provided, suggesting that M. rebeli is wholly parasitic on all stages in its host colony. The results also confirmed the importance of sucrose in the diet of Myrmica, and showed that M. rebeli caterpillars which eat ant brood to supplement their normal trophallactic feeding by workers develop more quickly - but have the same survival and pupal weights – as caterpillars that are fed solely by worker ants.
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  • 40
    ISSN: 1572-879X
    Schlagwort(e): aluminum oxide ; hydroxyl groups ; rhodium ; growth ; scanning tunneling microscopy ; infrared spectroscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Notizen: Abstract In order to investigate how the presence of surface hydroxyl groups on oxide surfaces affects the interaction with the supported metal, we have modified a well-ordered alumina film on NiAl(110) by Al deposition and subsequent exposure to water. This procedure yields a hydroxylated alumina surface as revealed by infrared and high-resolution electron energy loss spectroscopy. By means of scanning tunneling microscopy, we have studied the growth of rhodium on the modified film at 300 K. Clear differences in the particle distribution and density are observed in comparison to the clean substrate. While, in the latter case, decoration of domain boundaries as typical defects of the oxide film governs the growth mode, a more isotropic island distribution and a drastically increased particle density is found on the hydroxylated surface. From infrared data, it can be deduced that the growth is connected with the consumption of the hydroxyl groups due to the interaction between the metal deposit and the hydroxylated areas. This finding is in line with photoemission results published earlier.
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  • 41
    Digitale Medien
    Digitale Medien
    Springer
    Small business economics 14 (2000), S. 195-210 
    ISSN: 1573-0913
    Schlagwort(e): growth ; manufacturing ; performance ; product innovation ; small firms
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Wirtschaftswissenschaften
    Notizen: Abstract The paper considers the relative performance [along a number of parameters] of a sample of 228 small manufacturing firms categorised by level of innovation. Whilst innovators appear no more likely to have experienced some form of sales or employment growth, they are significantly more likely to have grown more. In other words, the innovators' growth rate distributions are highly negatively skewed. With regards to export intensities, profitability and productivity levels, the findings are less clear. On the whole, the results reported here are similar to those of other small firm studies, yet vary markedly from large firm equivalents; suggesting that the nature of the returns to innovation may be contingent, at least in part, upon firm size. Moreover, the high levels of variation in firm performance should caution us against proffering innovative imperatives. If we are to counsel firms to "innovate at all costs", we must be clear about, and clearly demonstrate, the nature of the returns they may reasonably expect and the processes through which these may be optimised.
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  • 42
    ISSN: 1572-9702
    Schlagwort(e): Panonychus citri ; spider mite ; microsatellite ; RAPD ; PCR ; DNA polymorphism
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Genetic markers were searched using PCR with 40 kinds of decanucleotide primers to investigate DNA polymorphism in Panonychuscitri. A region consisting of a variable number of CT tandem repeats (microsatellite) was found in a fragment amplified with the OPB10 primer. The microsatellite differed in size by ca. 100 bp among several P. citri populations screened and was derived from at least seven alleles. This region was characteristic of P. mori and P. osmanthi, but was lacking in P. ulmi. The flanking regions were highly conserved among these species.
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  • 43
    ISSN: 1572-9737
    Schlagwort(e): allozyme ; microgeographic divergence ; microsatellite ; natural selection ; RAPD ; Triticum dicoccoides ; wild emmer wheat
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The levels of genetic diversity were compared by means of 35 allozyme, 60 RAPD, and 25 microsatellite (SSR) markers for 75–175 individuals of tetraploid wild emmer wheat (Triticum dicoccoides) collected in 1993 from a microgeographic microsite, Ammiad, north of the Sea of Galilee, Israel. This microsite included four major habitats, which showed highly significant differentiation in ecological factors, in particular with respect to rock cover, proximity and height, and surface soil moisture in the early growing season of T. dicoccoides. Higher within-subpopulation genetic diversity was found in the primarily non-coding DNA regions (RAPD and SSR) rather than in the protein-coding (allozymes) regions. However, much larger gene differentiation (G ST) among the subpopulations was observed in the protein-coding allozymes than in the RAPDs and SSRs. Larger genetic distance was found at SSR loci, followed by allozyme and RAPD loci. The subpopulations in drier habitats tend to have higher allozyme, RAPD and SSR diversities (He), the relatively wet Karst subpopulation showed only about half He of the other relatively drier habitats. The subpopulations with larger difference of soil moisture between habitats tend to show larger genetic distances at allozyme, RAPD and SSR loci. These results suggest that climatic selection through aridity stress may be an important factor acting on both structural protein-coding and presumably partly regulatory non-coding DNA regions, resulting in microscale adaptive patterns, although hitchhiking and random drift may also intervene. These results have profound implications for genetic conservation both in situ and ex situ.
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  • 44
    ISSN: 1572-9737
    Schlagwort(e): Australia ; conservation strategy ; cpDNA ; Euphorbiaceae ; Fontainea ; nrDNA ; RAPD
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Four new eastern Australian Fontainea species have beenrecently described and all have a limited distribution. F.oraria is the rarest, being restricted to 10 adult individualswithin a single site in regrowth littoral rainforest. In order todevelop adequate management strategies, this study was aimed atsurveying the genetic variability remaining within the species by usingRAPD analysis. To assist with the correct interpretation of the results,a matching study was conducted on four populations of the closelyrelated F. australis. Similar amounts of within-populationgenetic diversity were recorded for both species. The RAPD-based studysuggested that adult plants are contributing unevenly to successivegenerations. RAPD analysis also recognised a close evolutionaryrelationship between F. oraria and F. australis.Sequencing of cpDNA (trnL-F) and nrDNA (ITS2) regions,confirmed recent divergence and possibly some historical reticulationbetween these two species and two other members of the genus. Ofparticular interest was the recognition that one of the F.australis populations (Limpinwood) represented a novel genotypiccombination in need of conservation attention. The implications of theRAPD and sequencing results are discussed in reference to theirinfluence upon the development of adequate conservation strategies forall important conservation units.
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  • 45
    Digitale Medien
    Digitale Medien
    Springer
    Sexual plant reproduction 12 (2000), S. 353-359 
    ISSN: 1432-2145
    Schlagwort(e): Key words Rosa sect Caninae ; Heterogamy ; Apomixis ; RAPD ; Pollen viability
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  All members of Rosa section Caninae, dogroses are polyploid and characterized by their unbalanced meiosis, which in most cases leads to a pronounced morphological influence from the maternal parent. In a previous investigation on a pair of reciprocal crosses between two species in this section, Rosa dumalis and R. rubiginosa (2n=35), nine offspring plants (approximately 10%) did not receive any of the 21 RAPD markers present in the respective pollen parent. This was interpreted as a possible occurrence of apomixis. These nine plants have now been subjected to a further study with additional markers. Thirteen new RAPD markers showed the same result as in the previous investigation: none of the nine plants inherited any of the pollen donor markers. The reproducibility of the RAPD markers was checked by mixing DNA samples to obtain a series of artificial hybrids between the two parent plants. Twelve RAPD markers gave the expected result, whereas one marker appeared only 50% of the time. In addition, pollen viability, mean number of seeds per hip, mean seed weight, and mean weight of fruit flesh per hip have been studied on the four progeny groups: R. dumalis×R. rubiginosa plants which received pollen donor markers (PM plants), R. dumalis×R. rubiginosa plants which did not receive any pollen donor markers (NPM plants), R. rubiginosa×R. dumalis PM plants and R. rubiginosa×R. dumalis NPM plants. A canonical discriminant analysis based on these four reproductive characters separated the four progeny groups. There were significant differences between the two PM groups in all investigated characters, and also between the PM and the NPM groups in pollen viability. The result from the RAPD markers together with the differences in pollen viability between the PM and NPM progeny groups is taken as an indication that apomixis occurs within the Caninae section.
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  • 46
    Digitale Medien
    Digitale Medien
    Springer
    Journal of population economics 13 (2000), S. 403-424 
    ISSN: 1432-1475
    Schlagwort(e): JEL classification: O41 ; F22 ; Key words: Altruism ; education ; growth ; convergence ; capital mobility
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Sociologie , Wirtschaftswissenschaften
    Notizen: Abstract. The aim of this paper is to discuss the process of regional convergence within the framework of an overlapping generations model in which the engine of growth is the accumulation of human capital. In particular, we consider different education funding systems and compare their performance in terms of growth rates and pace of convergence between two heterogeneous regions. The analysis suggests that the choice of a particular education system incorporates a possible trade-off between long run growth rate and short run convergence. In such choice, the initial capital stock and the extent of regional human capital discrepancy appear as central variables.
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  • 47
    Digitale Medien
    Digitale Medien
    Springer
    Theoretical and applied genetics 100 (2000), S. 1155-1166 
    ISSN: 1432-2242
    Schlagwort(e): Key words Citrus ; RAPD ; SCAR ; cpDNA ; Phylogeny ; Origin
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Citrus phylogeny was investigated using RAPD, SCAR and cpDNA markers. The genotypes analyzed included 36 accessions belonging to Citrus together with 1 accession from each of the related genera Poncirus, Fortunella, Microcitrus and Eremocitrus. Phylogenetic analysis with 262 RAPDs and 14 SCARs indicated that Fortunella is phylogenetically close to Citrus while the other three related genera are distant from Citrus and from each other. Within Citrus, the separation into two subgenera, Citrus and Papeda, designated by Swingle, was clearly observed except for C. celebica and C. indica. Almost all the accessions belonging to subgenus Citrus fell into three clusters, each including 1 genotype that was considered to be a true species. Different phylogenetic relationships were revealed with cpDNA data. Citrus genotypes were separated into subgenera Archicitrus and Metacitrus, as proposed by Tanaka, while the division of subgenera Citrus and Papeda disappeared. C. medica and C. indica were quite distant from other citrus as well from related genera. C. ichangensis appeared to be the ancestor of the mandarin cluster, including C. tachibana. Lemon and Palestine sweet lime were clustered into the Pummelo cluster led by C. latipes. C. aurantifolia was located in the Micrantha cluster. Furthermore, genetic origin was studied on 17 cultivated citrus genotypes by the same molecular markers, and a hybrid origin was hypothesized for all the tested genotypes. The assumptions are discussed with respect to previous studies; similar results were obtained for the origin of orange and grapefruit. Hybrids of citron and sour orange were assumed for lemon, Palestine sweet lime, bergamot and Volkamer lemon, while a citron × mandarin hybrid was assumed for Rangpur lime and Rough lemon. For Mexican lime our molecular data indicated C. micrantha to be the female parent and C. medica as the male one.
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  • 48
    Digitale Medien
    Digitale Medien
    Springer
    Theoretical and applied genetics 100 (2000), S. 1209-1216 
    ISSN: 1432-2242
    Schlagwort(e): Key words Digitalis spp. ; AMOVA ; Genetic relationships ; RAPD ; Scrophulariaceae
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  RAPD markers were used to study inter-specific variation among six species of the genus Digitalis: D. obscura, D. lanata, D. grandiflora, D. purpurea, D. thapsi and D. dubia, and the hybrid D. excelsior (D. purpurea×D. grandiflora). A total of 91 highly reproducible bands amplified with four arbitrarily chosen decamer primers were obtained. Homology of the co-emigrating RAPD markers was tested by blot hybridisation and sequencing of selected bands. The application of a range of statistical approaches for RAPD data analysis, including distance and parsimony methods, family clustering and the analysis of molecular variance (AMOVA), indicated that these molecular markers were taxonomically informative in Digitalis. The species relationships revealed were fully consistent with those previously obtained using morphological affinities. The hybrid D. excelsior seems to have stronger affinity to the section Digitalis than to Grandiflorae. This is the first known report of the application of RAPD markers for the study of genetic relationships among species of the genus Digitalis.
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  • 49
    Digitale Medien
    Digitale Medien
    Springer
    Theoretical and applied genetics 101 (2000), S. 70-79 
    ISSN: 1432-2242
    Schlagwort(e): Key words Poa annua L. ; Genetic diversity ; RAPD ; Turfgrass weeds ; Selection pressure ; Analysis of molecular variance ; AMOVA ; POPGENE
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  The genetic diversity of Poa annua L.populations collected from western Oregon grass-seed fields was surveyed using 18 randomly amplified polymorphic DNA (RAPD) markers. Markers from 1357 individual plants from 47 populations collected at three sampling dates (fall, winter, and spring) for 16 sites were used to measure genetic diversity within and among populations. Site histories varied from low to high herbicide selection pressure, and some sites were subdivided by 3 years of differing post-harvest residue management. Gene diversity statistics, simple frequency of haplotype occurrence, and analysis of molecular variance (AMOVA) revealed the presence of significant variability in P. annua among sites, among collection dates within sites, and within collection dates. Nei gene-diversity statistics and population-differentiation parameters indicated that P. annua populations were highly diverse. Mean Nei gene diversity (h) for all 47 populations was 0.241 and total diversity (HT) was 0.245. A greater proportion of this diversity, however, was within (HS=0.209) rather than among (GST=0.146) populations. When populations were grouped by season of collection, within-group diversity was HS=0.241, while among-group diversity was GST=0.017. When populations were grouped by site, within-group diversity was HS=0.224, while among-group diversity was GST=0.087. The diversity among populations within season for fall, winter, and spring collections was GST=0.121, 0.142, and 0.133, respectively. Populations collected from fields with histories of high herbicide selection pressure showed low differentiation among collection dates, with GST as low as 0.016, whereas those collected from fields with low herbicide selection pressure showed greater differentiation among collection dates, with GST as high as 0.125. At high selection-pressure sites, populations were also lower in gene diversity (as low as h=0.155), while at low selection-pressure sites there was higher gene diversity (as high as h=0.286). The site to site variability was greater for the high selection-pressure sites (GST=0.107 or 69% of the total among-population variance), while the season of germination variability was greater at sites of low herbicide-selection pressure (GST=0.067, or 70% of the total among-population variance). High initial diversity coupled with a long-term re-supply of genotypes from the seed bank must have been factors in maintaining the genetic diversity of this weed despite the intensive use of herbicides. Knowledge of the genetic diversity of Willamette Valley P. annua should help in formulating more effective strategies for managing this weed.
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  • 50
    Digitale Medien
    Digitale Medien
    Springer
    Theoretical and applied genetics 101 (2000), S. 90-94 
    ISSN: 1432-2242
    Schlagwort(e): Key words Molecular map ; AFLP ; RAPD ; Optimisation algorithm
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  A computer algorithm is presented which allows selection of a subset of multiplex markers based on the minimisation of an optimality criterion for a genetic linkage map. It could be applied for choosing a subset of primers (e.g. RAPD, IMA or AFLP), each of which provides several unevenly spaced genetic markers. The goal is to achieve a saturated map of evenly spaced markers, using as few primers as possible to minimise cost and labour. Minimising the average map distance between markers is trivial, but simply leads to selection of those primers which provide the greatest number of markers. However, minimising the standard deviation of interval length ensures that weight is given both to the number of markers and to the evenness of their distribution on the linkage map. This criterion was found empirically to give a result fairly close to the optimum. A stepwise-like selection procedure is therefore implemented, which stops when the optimality criterion does not decrease any more. An example is given of a molecular map of perennial ryegrass with 463 markers obtained from 17 AFLP primers. It is demonstrated that this can be safely reduced to a 175 marker map with only 6 primers. Genetic diversity studies may also benefit from using such a subset of less-redundant markers in genetic distance estimation.
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  • 51
    Digitale Medien
    Digitale Medien
    Springer
    Theoretical and applied genetics 101 (2000), S. 292-300 
    ISSN: 1432-2242
    Schlagwort(e): Key words AFLP ; DNA markers ; Early germination ; ISSR ; ISTR ; RAPD
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Different DNA marker types were used to construct linkage maps in coconut (Cocos nucifera L.; 2n = 32) for the two parents of the cross Malayan Yellow Dwarf (MYD) × Laguna Tall (LAGT). A total of 382 markers was sufficient to generate 16 linkage groups for each parent. The total genome length corresponded to 2226 cM for the LAGT map and 1266 cM for the MYD map with 4–32 markers per linkage group. Common markers allowed the association of 9 linkage groups for the two parents MYD and LAGT. QTL analysis for the trait early germination identified six loci. These QTLs correlate with early flowering and yield, representing characters which are important in coconut breeding. The co-segregation of markers with these QTLs provides the first opportunity for marker-assisted selection in coconut breeding programmes.
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  • 52
    Digitale Medien
    Digitale Medien
    Springer
    Theoretical and applied genetics 100 (2000), S. 614-620 
    ISSN: 1432-2242
    Schlagwort(e): Key words Aigeiros ; Leuce ; Marssonina brunnea ; Poplar ; RAPD ; Tacamahaca
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  A broad collection was made for 42 isolates of Marssonina brunnea affecting poplar trees from three different sections (Leuce, Aigeiros and Tacamahaca) within the same Populus genus in China. Genetic diversity among these isolates was analyzed for morphological traits, cultural features, pathogenicity, hyphal anastomosis and randomly amplified polymorphic DNA markers (RAPDs). No significant difference was found in conidial morphological features, such as size, shape and septum location. Yet, considerable differences occur in other characteristics, which leads to the classification of the 42 isolates into two distinct groups, M. brunnea f.sp. monogermtubi and M. brunnea f.sp. multigermtubi. Isolates of M. brunnea f.sp. monogermtubi, derived from section Leuce, germinate only one germ tube, grow fast, produce dark-reddish conidiosorus clusters on the PDA medium, and are highly pathogenic to Populus tomentosa of section Leuce. By contrast, isolates of M. brunnea f.sp. multigermtubi, derived from sections Aigeiros and Tacamahaca, germinate 1–5 germ tubes, grow slowly, produce yellow-greenish conidiosorus clusters on PDA medium, and are pathogenic to Populus ×euramericana cv I-45 and Populus canadensis of section Aigeiros. DNA amplification using 11 RAPD primers generate 78 polymorphic bands among isolates. Cluster analyses based on RAPD markers broadly support such a classification by phenotypes, but provide a new insight into the possible origins of M. brunnea. It is proposed that the pathogen co-evolves with the poplars of section Leuce and has been subsequently distributed to the poplars of sections Aigeiros and Tacamahaca. An isolate from Populus adenopoda of section Leuce is placed in the third group, which is most likely a transmission type from M. brunnea f.sp. monogermtubi to M. brunnea f.sp. multigermtubi.
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  • 53
    ISSN: 1432-2242
    Schlagwort(e): Key words SCAR ; RAPD ; Bulked segregant analysis ; Marker-assisted selection ; Orobanche cumana ; Helianthus annuus
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  A consensus molecular linkage map of 61.9 cM containing the Or5 gene, which confers resistance to race E of broomrape orobanche cumana, five SCAR markers (three dominant, two codominant) and one RAPD marker were identified based on segregation data scored from two F2 populations of susceptible×resistant sunflower line crosses. Bulked segregant analysis was carried out to generate the five SCAR markers, while the single RAPD marker in the group was identified from 61 segregating RAPD markers that were directly screened on one of the two F2 populations. The five SCAR markers, RTS05, RTS28, RTS40, RTS29 and RTS41, were significantly (LOD≥4.0) linked to the Or5 gene and mapped separately at 5.6, 13.6, 14.1, 21.4 and 39.4 cM from the Or5 locus on one side, while the RAPD marker, UBC120_660, was found at 22.5 cM (LOD=1.4) on the opposite side. These markers should facilitate the efficient transfer of the resistance gene among sunflower breeding lines. As the first report on molecular markers linked to a broomrape resistance gene, the present work provides a starting point to study other genes and to examine the hypothesis of the clustering of broomrape resistance genes in sunflower.
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  • 54
    ISSN: 1432-2242
    Schlagwort(e): Key words TGMS ; RAPD ; AFLP ; Microsatellites ; STS ; Marker-assisted selection ; Bulked seg analysis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  The present study of genetic analysis is an attempt to precisely characterize diverse temperature-sensitive genic male-sterile (TGMS) lines so as to explore the possibilities of utilizing the most promising in large-scale hybrid seed production. Genetical studies revealed that the TGMS segregants derived from crosses involving TGMS lines ID24 and SA2 expressed differential fertility levels at low-temperature conditions. A majority of these progenies expressed transgressive segregation towards either sterility of fertility, causing instability of sterility and low reversibilty of fertility which may be due to large numbers of single-locus QTLs and their epistatic interactions. We identified two putative genes imparting temperature-sensitive male sterility after observing crosses involving diverse TGMS sources. To identify suitable molecular markers closely linked to the trait we used RAPD, AFLP and microsatellites which generated polymorphism through bulked segregant analysis. AFLP analysis using a smaller genome kit resulted in enormous polymorphism, out of which the combination EAA/MCAG amplified a 330-bp fragment, which closely segregated with the gene at a distance of 5.3 cM. This fragment was eluted for cloning and from the sequence a STS primer (TS200) was developed which produced a dominant polymorphism specific to TGMS. The microsatellite RM257, located earlier on chromosome 9, was linked with the TGMS trait in SA2 at a distance of 6.2 cM. RM257 produced a codominant polymorphism with 145-bp (sterile) and 132-bp (fertile) products. Both individually and collectively, the markers TS200 and RM257 located on either side of the TGMS locus are very useful for marker-assisted selection.
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  • 55
    Digitale Medien
    Digitale Medien
    Springer
    Theoretical and applied genetics 100 (2000), S. 965-970 
    ISSN: 1432-2242
    Schlagwort(e): Key words Russian wheat aphid ; Near-isogenic lines ; Restriction digests ; RAPD ; SCAR
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstracts  Through random amplified polymorphic DNA (RAPD) analysis we identified a putative marker linked to the Dn5 resistance gene. This marker was converted to a more reliable sequence-characterised-amplified regions (SCAR) marker. The initial SCAR marker amplified the correct amplification product but failed to discern between the susceptible and resistant individuals. Hence, it was utilised to sequence the internal fragment. All nested primers designed from the internal sequences were also unable to produce any polymorphism between the susceptible and resistant cultivars. Restriction digests were then performed on these fragments, and the restriction enzyme EcoRI was able to discern between the susceptible and resistant F2 individuals of the Dn5 population. This granted one marker amplified with the internal SCAR primer set OPF141083 the ability to differentiate between parental individuals carrying the Dn5 genes. This marker was tested in a segregating F2 population carrying the Dn5 resistance gene and proved able to differentiate between the segregating individuals. This marker may prove useful in marker assisted selection (MAS), although performing restriction digests may hamper the throughput of a high number of samples.
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  • 56
    ISSN: 1432-2242
    Schlagwort(e): Key words Pinus pinaster ; AFLP ; RAPD ; Protein ; Linkage map ; QTL
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  TheAFLP (amplified fragment length polymorphism) technique was adapted to carry out genetic analysis in maritime pine, a species characterized by a large genome size (24 pg/C). A genetic linkage map was constructed for one F1 individual based on 239 AFLP and 127 RAPD (randomly amplified polymorphic DNA) markers. Markers were scored on megagametophytes (1n) from 200 germinated F2 seedlings. Polymorphism rate, labour time and cost of both AFLP and RAPD techniques were compared. The AFLP technique was found to be twice as fast and three-times less costly per marker than the RAPD technique. Thirteen linkage groups were identified with a LOD score ≥6 covering 1873 cM, which provided 93.4% of genome coverage. Proteins were extracted from needles (2n) of the F2 progeny and revealed by 2-DE (two-dimensional electrophoresis). Thirty one segregating proteins were mapped using a QTL detection strategy based on the quantification of protein accumulation. Two framework maps of the same F1 individual are now available. The first map (Plomion et al. 1996) uses RAPD markers and the second map, presented in this study, uses mostly AFLP markers. Although the total genetic length of both maps was almost identical, differences among homologous groups were observed.
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  • 57
    Digitale Medien
    Digitale Medien
    Springer
    Theoretical and applied genetics 100 (2000), S. 63-70 
    ISSN: 1432-2242
    Schlagwort(e): Key words Elaeis guineensis ; RAPD ; Pseudo-testcross ; Genetic linkage map ; bulked segregant analysis ; Shell thickness
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Shell thickness is an important trait in oil palm breeding programs and is the basis for the classification of the varieties of oil palm into the types dura, tenera and pisifera. This trait seems to be controlled by a single locus, with two alleles (sh + and sh −) showing codominant expression. Two single-tree linkage maps were constructed for a maternal tenera (sh + sh −) palm and for a paternal pisifera (sh − sh −) palm using the pseudo-testcross mapping strategy in combination with RAPD markers through the analysis of an F1 tenera×pisifera progeny. A total of 308 arbitrary primers were screened in a sample of eight F1 plants and 121 markers were detected in a testcross configuration. An average of 1.66 polymorphic marker per selected primer were identified in this cross. At LOD 5.0 (with some few exceptions) and θ=0.25 the maternal tenera map included a total of 48 markers distributed in 12 linkage groups or pairs of markers (449.3 cM) while the paternal pisifera map included 42 markers distributed in 15 linkage groups or pairs of markers (399.7 cM). We used RAPD and bulked segregant analysis (BSA) to identify markers more tightly linked to the sh + locus. A total of 174 new primers not previously used in the linkage analysis were screened using bulks of DNA extracted from plants selected for the contrasting shell-thickness phenotypes. Two RAPD markers (R11–1282 and T19–1046) were identified to be linked on both sides of the sh + locus on linkage group 4. The estimated map distances from sh + to R11–1282 and to T19–1046 were 17.5 cM and 23.9 cM, respectively. The results demonstrate the usefulness of RAPD markers and the pseudo-testcross mapping strategy for developing genetic linkage information, and constitute an important step towards early marker-assisted selection for shell thickness in oil palm.
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  • 58
    Digitale Medien
    Digitale Medien
    Springer
    Theoretical and applied genetics 100 (2000), S. 249-255 
    ISSN: 1432-2242
    Schlagwort(e): Key words QTL ; Earliness ; CAP ; RAPD ; Lycopersicon esculentum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Using RAPD marker analysis, two quantitative trait loci (QTLs) associated with earliness due to reduced fruit-ripening time (days from anthesis to ripening = DTR) were identified and mapped in an F2 population derived from a cross between Lycopersicon esculentum’E6203’ (normal ripening) and Lycopersicon esculentum’Early Cherry’ (early ripening). One QTL, on chromosome 5, was associated with a reduction in both ripening time (5 days) and fruit weight (29.3%) and explained 15.8 and 13% of the total phenotypic variation for DTR and fruit weight, respectively. The other QTL, on chromosome 12, was primarily associated with a reduction only in ripening time (7 days) and explained 12.3% of the total phenotypic variation for DTR. The gene action at this QTL was found to be partially dominant (d/a=0.41). Together, these two QTLs explained 25.1% of the total phenotypic variation for DTR. Additionally, two QTLs associated with fruit weight were identified in the same F2 population and mapped to chromosomes 4 and 6, respectively. Together, these two QTLs explained 30.9% of the total phenotypc variation for fruit weight. For all QTLs, the ’Early Cherry’ alleles caused reductions in both ripening time and fruit weight. The polymorphic band for the most significant RAPD marker (OPAB-06), linked to the reduced ripening time QTL on chromosome 12, was converted to a cleaved amplified polymorphism (CAP) assay for marker-aided selection and further introgression of early ripening time (DTR) into cultivated tomato.
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  • 59
    Digitale Medien
    Digitale Medien
    Springer
    Theoretical and applied genetics 100 (2000), S. 299-307 
    ISSN: 1432-2242
    Schlagwort(e): Keywords Larix ; Linkage map ; RAPD ; AFLP ; ISSR ; Genetic mapping
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Genetic linkage maps have been increasingly developed for a wide variety of plants, using segregating populations such as F2s or backcrosses between inbred lines. These pedigrees are rarely available in outbred species like forest trees which have long generation times. Thus genetic mapping studies have to use peculiar pedigrees and markers in appropriate configurations. We constructed single-tree genetic linkage maps of European larch (Larix decidua Mill.) and Japanese larch [Larix kaempferi (Lamb.) Carr.] using segregation data from 112 progeny individuals of an hybrid family. A total of 266 markers (114 AFLP, 149 RAPD and 3 ISSR loci) showing a testcross configuration, i.e.heterozygous in one parent and null in the other parent, were grouped at LOD 4.0, θ=0.3. The maternal parent map (L. decidua)consisted of 117 markers partitioned within 17 linkage groups (1152 cM) and the paternal parent map (L. kaempferi) had 125 markers assembled into 21 linkage groups (1206 cM). The map distance covered by markers was determined by adding a 34.7-cM independence distance at the end of each group and unlinked marker. It reached 2537 cM and 2997 cM respectively for European larch and Japanese larch, and represented respectively a 79.6% and 80.8% coverage of the overall genome. A few 3:1 segregating markers were used to identify homologous linkage groups between the European larch and the Japanese larch genetic maps. The PCR-based molecular markers allowed the construction of genetic maps, thus ensuring a good coverage of the larch genome for further QTL detection and mapping studies.
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  • 60
    Digitale Medien
    Digitale Medien
    Springer
    Theoretical and applied genetics 101 (2000), S. 1145-1154 
    ISSN: 1432-2242
    Schlagwort(e): Keywords Altitude differentiation ; Environmental selection ; Phytolacca dodecandra ; RAPD
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  The extent of genetic differentiation among 17 Ethiopian populations (249 individuals) of Phytolacca dodecandra (Endod) sampled along altitudinal gradients that varied from 1600 to 3000 m was investigated using random amplified polymorphic DNA (RAPD). The populations were classified into three altitude groups: lowland (1600–2100 m), central-highland (2101–2500 m) and highland (2500–3000 m). Seventy polymorphic loci scored from 12 RAPD primers, singly or in combination with ecogeographical variables (altitude, longitude, latitude, temperature and rainfall), were used for principal component, discriminant, correlation, and stepwise multiple regression analyses. Principal component analysis (PCA) clearly differentiated lowland and the central-highland populations from those of the highlands independent of their geographical regions. Canonical discriminant analysis separated the lowland plants from those of the highlands with the central-highland plants being intermediate. Classificatory discriminant analysis corrected classification of 92.8% of the 249 plants into their respective three altitude groups. Multiple regression analysis identified a strong association between some RAPDs and altitude, temperature and rainfall, while the variation in most RAPDs was explained by combinations of the different ecogeographical variables. It is hypothesised that the different altitude groups may be (1) chemical and/or physiological ecotypes produced as a result of complex interactions of altitude with climatic and/or edaphic factors, or (2) different in ploidy levels. The significant correlations obtained between population means from some RAPDs and altitude and temperature as well as the strong association of some RAPDs with the ecogeographical variables in the multiple regression analysis suggest that part of the RAPD polymorphism could be adaptive, and responsive to environmental selection.
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  • 61
    ISSN: 1432-2242
    Schlagwort(e): Key words Gene diversity ; Isozyme ; Non-neutrality ; RAPD ; Rosaceae
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  RAPD and isozyme analyses based on numerous markers have been used for the first time to investigate patterns of phenetic and genetic differentiation among and within nine wild populations of the genus Chaenomeles represented by the species C. japonica, C. speciosa, C. cathayensis and C. thibetica. Highly significant correlations were found between the two different marker systems for both phenetic distances and gene diversity estimates. In agreement with previous studies on cultivated Chaenomeles material, C. japonica was clearly differentiated from C. speciosa and C. cathayensis. The recently recognised species C. thibetica appeared to be rather closely related to C. cathayensis. Populations of C. japonica and C. speciosa were considerably more diverse than populations of C. cathayensis and C. thibetica. Correspondingly, most of the total variability could be attributed to the within-population differentiation in the case of C. japonica and C. speciosa, and to the between-population differentiation in the case of C. cathayensis. Differences in mating systems among the species can be suggested as a possible explanation of the results. A discordant pattern was found between RAPDs and isozymes in the analyses of population structure within C. japonica. This may be explained by a higher proportion of non-neutral markers for isozymes than for RAPDs. This finding also shows the importance of using multiple molecular marker systems in studies of population structure.
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  • 62
    Digitale Medien
    Digitale Medien
    Springer
    Theoretical and applied genetics 100 (2000), S. 506-511 
    ISSN: 1432-2242
    Schlagwort(e): Key words Rye ; DNA instability ; Hypervariable sequences ; Somaclonal variation ; RAPD
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  RAPD analysis was performed to assess DNA variation among rye plants regenerated from immature embryos and inflorescences. From the studied plants, 40% showed at least one variation, and the number of mutations per plant was quite high, ranging from 1 up to 12. On some occasions (2.9% of the scored bands) the modified band was observed in only one plant or in several but originated from the same callus (variable band). In other cases (5.25%) the same band varied in several plants obtained from different calli. We call these hypervariable bands and they could vary between plants belonging to different cultivars and/or with different origins, inflorescences or embryos. Thus, they must originate through independent mutational events. We assume that these bands represent hypervariable regions of the rye genome and so detect hot spots of DNA instability. Some of these bands proved to be unique sequences, others were present in a low copy number while the remaining ones were moderately or highly repetitive.
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  • 63
    Digitale Medien
    Digitale Medien
    Springer
    Theoretical and applied genetics 101 (2000), S. 1194-1201 
    ISSN: 1432-2242
    Schlagwort(e): Key words ’Folle blanche’ ; Hybridization ; RAPD ; SCAR ; Sequence-specific primer pair ; Vitis vinifera L
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Among 34 grapevine cultivars (Vitis vinifera L.), eight putative genotype-specific RAPD markers, from ’Albariño’, ’Caíño blanco’, ’Chardonnay’, ’Folle blanche’, ’Grenache blanc’, ’Malvasía Sitges’, ’Torrontés’ and ’Treixadura’ respectively, were selected to transform into SCAR markers. Of these, seven markers were cloned and then five which showed a positive specific hybridization signal were sequenced. For these five markers, 30 sequence-specific primers ranging from 14 to 29 bases were designed to amplify genomic DNA from 64 grapevine cultivars under more-stringent PCR conditions. Only, two primer pairs, OpA111175p17R/ p17F and OpD10800p14R/p14F, still produced a specific SCAR marker, the ’Folle blanche’ ScA111175 and the ’Malvasía Sitges’ ScD10800 respectively. Moreover, the ScA111175 marker was amplified only in ’Folle blanche’ among the 64 cultivars tested with a large annealing temperature range using either two different Taq DNA polymerases or two separate thermocyclers. In addition, we discuss the initial polymorphism originated by the RAPD technique and suggest a new design of SCAR primers to obtain reliable cultivar-specific SCAR markers from single PCR-based bands for identification purposes.
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  • 64
    Digitale Medien
    Digitale Medien
    Springer
    Theoretical and applied genetics 101 (2000), S. 860-864 
    ISSN: 1432-2242
    Schlagwort(e): Key words Melon ; AFLP ; RFLP ; RAPD ; Genetic similarity
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Three different types of molecular markers, RAPD, AFLP and RFLP were used to measure genetic diversity among six genotypes of Cucumis melo L. Each line represented a different melon genotype: Piel de Sapo, Ogen, PI161375, PI414723, Agrestis and C105. A number of polymorphic RAPD, AFLP and RFLP bands were scored on all materials and the genetic similarity measured. Clustering analysis performed with the three types of markers separated the genotypes into two main groups: (1) the sweet type, cultivated melons and (2) the exotic type, not cultivated melons. While the data obtained suggest that all three types of markers are equally informative, AFLPs showed the highest efficiency in detecting polymorphism.
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  • 65
    Digitale Medien
    Digitale Medien
    Springer
    Theoretical and applied genetics 101 (2000), S. 1056-1065 
    ISSN: 1432-2242
    Schlagwort(e): Key words Plantain ; Musa ; RAPD ; Phenotype ; Breeding
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Genetic diversity amongst 76 plantain landraces has been studied using RAPD analysis at two levels of intensity and compared with groupings based on phenotypic indices and morphotype. There was a good correlation (R2=0.78) between estimates of genetic diversity based on 76 RAPD bands and 164 RAPD bands. However, there was a poor correlation between RAPD-based estimates of genetic diversity and a phenotypic index based on agronomic characters. There was also a poor correlation between RAPD analyses and morphotype group (based on bunch type and stature). These results suggest that the traditional designations of plantain landraces based on morphotype do not provide a true reflection of overall genetic divergence. Similarly, classification systems using phenotypic indices based on agronomic characters may not provide accurate taxonomic differentiation. The level of genetic divergence within morphogroups based on bunch type suggests that True Horn plantains are derived from False Horn plantains which in turn are derived from French plantains. Genetic divergence was found to be generally quite low within the plantain landrace genepool, which is consistent with the proposed evolution of this germplasm through somatic mutation of a relatively small number of introductions. However, putative synonyms/duplicates have been shown to be genetically distinct. In contrast, a group of 12 landraces have been identified that are highly distinct from one another (showing 20–35% dissimilarity). Fertile members of this group may be useful for generating genetically diverse 2x and 4x breeding populations that can be used in breeding secondary triploid hybrid plantain varieties.
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  • 66
    Digitale Medien
    Digitale Medien
    Springer
    Theoretical and applied genetics 101 (2000), S. 931-938 
    ISSN: 1432-2242
    Schlagwort(e): Key words Gossypium species ; RAPD ; Phylogeny ; Cluster ; Diversity
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Total genomic DNA from 31 available Gossypium species, three subspecies and one interspecific hybrid, were analysed to evaluate genetic diversity by RAPD, using 45 random decamer primers. A total of 579 amplified bands were observed, with 12.9 bands per primer, of which 99.8% were polymorphic. OPJ-17 produced the maximum number of fragments while the minimum number of fragments was produced with primer OPA-08. Cluster analysis by the unweighted paired group method of arithmetic means (UPGMA) showed six main clusters. Cluster ’A’ consisted of two species and one subspecies of the A-genome, with a 0.78–0.92 Nei’s similarity range. Cluster B, composed of all available tetraploid species and one interspecific hybrid, showed the same sister cluster. Nei’s similarity ranged from 0.69 to 0.84. The B-genome formed the UPGMA sister cluster to the E-genome species. Cluster ’C’ consisted of five Gossypium species of which three belong to the B-genome, with Nei’s similarity values of 0.81 to 0.86. Although there was considerable disagreement at lower infra-generic ranks, particularly among the D- genome (diploid New World species) and C-genome (diploid Australian species) species. The sole F-genome species Gossypium longicalyx was resolved as a sister group to the D-genome species. Gossypium herbaceum and G. herbaceum Africanum showed the maximum Nei’s similarity (0.93). Minimum similarity (0.29) was observed between Gossypium trilobum and Gossypium nelsonii. The average similarity among all studied species was 50%. The analysis revealed that the interspecific genetic relationship of several species is related to their centre of origin. As expected, most of the species have a wide genetic base range. The results also revealed the genetic relationships of the species Gossypium hirsutum to standard cultivated Gossypium barbadense, G. herbaceum and Gossypium arboreum. These results correspond well with previous reported results. The level of variation detected in closely related genotypes by RAPD analysis indicates that it may be a more efficient marker than morphological marker, isozyme and RFLP technology for the construction of genetic linkage maps.
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  • 67
    ISSN: 1570-0267
    Schlagwort(e): cDNA ; PCR cDNA ; TaqMan Analysis ; gene expression ; Pearson's correlation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Comparative gene expression studies are often limited by low availability of tissue and poor quality of extractable mRNA. Collective PCR amplification of minute quantities of mRNA has great potential for overcoming these limitations. However, there remains significant concern about the effects of amplification on the absolute and relative abundance of individual mRNAs that could complicate subsequent gene expression studies. To address this problem, we systematically compared the relative abundance of many specific mRNAs from complex cDNA preparations (from tissue and cultured cells) both before and after amplification by PCR. Our results demonstrated that, as expected, the absolute abundance of different mRNAs in a cDNA library is altered in an unpredictable manner by PCR amplification. However, we found that the concentration ratios of specific mRNAs among different cDNA preparations were routinely well conserved after PCR amplification. Thus, for the purpose of comparative expression studies for specific mRNAs in two (or more) complex cDNAs, PCR-amplified cDNA is equally useful as unamplified cDNA. These results provide a rigorous experimental validation and offer a theoretical treatment to support the utility of PCR amplified cDNA for differential gene expression studies. We conclude that the inherent difficulties in performing differential screening studies such as gene chip and array analyses on limited amounts of biological materials can be overcome by a PCR amplification step without compromising data quality.
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  • 68
    Digitale Medien
    Digitale Medien
    Springer
    Ecological research 15 (2000), S. 101-106 
    ISSN: 1440-1703
    Schlagwort(e): comparative ecology ; growth ; marine fish ; patterns ; reproduction
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: A number of strong regularities characterize certain very basic biological parameters in marine fishes. For example, the ovulated eggs of fish usually measure approximately 1 mm in diameter. The small, relatively uniform size of the eggs means that almost all fish larvae experience environmental variability at very similar scales, which itself establishes strong constraints for, and links between reproduction and recruitment. Additional constraints emerge from seawater being a poor medium for respiration, which establishes further linkages between growth and mortality. These constraints have produced strongly convergent features, and thence the patterns in reproduction and growth of marine fishes that are presented.
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  • 69
    ISSN: 1573-143X
    Schlagwort(e): body composition ; catfish (Mystus nemurus) ; feeding rate ; growth
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract A 10 week experiment was conducted to determine theeffects of feeding rate on growth, feed utilizationand body composition of the tropical bagrid catfish,Mystus nemurus. Catfish fingerlings with anaverage initial body weight of 12 g were fed apractical diet (36.2% protein, 16.5 kJ/g diet) atrates of 1, 2, 2.5, 3, 3.5, 4 or 5% of their bodyweight (BW) per day in two equal meals. Watertemperature was approximately 29 °C throughoutthe experiment. Percent weight gain increased almostlinearly with increasing feeding rates up to 2.5%BW/day beyond which no significant (P 〉 0.05)improvement in weight gain was observed. The specificgrowth rate of catfish fed rations of 1% BW/day was0.72%/day and this increased significantly to anaverage of 1.39%/day for catfish fed 2.5% BW/day andbeyond. Feed utilization did not differ significantly(P 〉 0.05) between fish fed 1.0 to 2.5%BW/day but decreased when rations were increased to3.0% BW/day and above. Feed efficiency ratio was0.79 for catfish fed 1.0% BW/day compared to a ratioof 0.27 for fish fed at 5% BW/day. Catfish fed 1.0%BW/day had the lowest condition factor, hepato- andviscerosomatic indices, but the highest carcass tobody weight ratio. These fish also had lowerproportions of whole body dry matter, lipid andprotein, carcass dry matter and lipid, and visceraldry matter and lipid than fish in other groups. Therewere no significant differences in either conditionindices or relative body composition of fish fedrations of 2.0 to 5.0% BW/day. Based on the growth,feed efficiency and body composition data obtained, afeeding rate of 2.5% BW per day is recommended forM. nemurus fingerlings raised at 29 °C.
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  • 70
    Digitale Medien
    Digitale Medien
    Springer
    Aquaculture international 8 (2000), S. 531-542 
    ISSN: 1573-143X
    Schlagwort(e): feed intake ; growth ; protein utilisation ; proximate ; composition ; rainbow trout (Oncorhynchus mykiss)
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Rainbow trout Oncorhynchus mykiss grew from 44 to 326 g in 96days when held at ∼12 °C. Fish were fed to satiation twice dailywith either high (L1: 30.8%, L2:31.4%) or lower-lipid feeds (C1: 18.8%,C2: 21.8%). Four feeding treatments were studied.Group C1C2 received feed C1 for 43 days(days 0–43) and C2 thereafter (days 44–96).Groups L1L2, L1C2 andC1L2 were subjected to dietary changes asindicated by the feed designations. After a short period of feedadaptation, fish ingested similar amounts of feed energy i.e., they ateless by weight of the lipid-rich (L) feeds. Feed lipid content did notaffect growth but fish fed L-feed had reduced feed conversion ratio(FCR) compared to fish fed C-feed (0.731 vs. 0.773) during days0–43 (P 〈 0.01). After 96 days,L1L2-fish were lower in body protein(15.8%) than the C1C2-fish (16.8%)(P 〈 0.01). L-feeds also tended to increase percentage lipidand reduce percentage whole body moisture and ash. A higher net proteinutilisation (NPU) was recorded in fish fed L-feeds (43.6%)compared to fish fed C-feeds (38.8%) in days 0–43(P 〈 0.05). This seemed to be the result of a lower proteinintake rather than a protein-sparing effect of feed lipid. Above athreshold value of approximately 6.5 mg protein eaten·g bodywtminus 1·day−1, NPU decreased.
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  • 71
    Digitale Medien
    Digitale Medien
    Springer
    Aquaculture international 8 (2000), S. 543-549 
    ISSN: 1573-143X
    Schlagwort(e): aggression ; Atlantic salmon (Salmo salar) ; dominance ; growth
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Aggression in groups of 0+ Atlantic salmon (Salmo salar) was monitoredat weekly intervals in two tanks containing 100 fish each. Three 1 + salmonparr were added to one of these. After 5 weeks, fish weights were measuredin both tanks and the conditions reversed. At ten weeks, weights of fish inboth tanks were measured again. In both populations, levels of aggressionamong the smaller fish were significantly lower and growth ratessignificantly higher when the large fish were present. Although the largefish attacked the small ones, the rate at which they did so was an order ofmagnitude lower that the rate at which small fish attacked each other in theabsence of larger conspecifics. This raises the possibility that levels ofaggression among farmed salmon might be reduced by the addition of a fewlarge conspecifics.
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  • 72
    ISSN: 1573-4919
    Schlagwort(e): myosin heavy chain ; gene expression ; hypertrophy ; dexamethasone ; promoter function
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract Cardiac hypertrophy has been observed in newborn infants treated with dexamethasone (DEX). This study was undertaken to examine whether DEX-induced hypertrophy in newborn rats is associated with redistribution of cardiac myosin heavy chain (MHC) isoforms and if so, the effects involve transcriptional regulation. Newborn rats were injected with either DEX (1 mg/kg/day; s.c.) or equivalent volume normal saline for 1, 3, 5, 7 or 9 days. Hypertrophy was quantified by heart dry/wet wt ratios, heart/body wt ratios, and total protein content of the myocardium. Changes in the expression of cardiac MHC mRNA were characterized by northern blot and slot blot analyses, using isoform specific probes for a- and β-MHC genes. DEX effect on α-MHC gene transcription was analyzed by transiently transfecting various α-MHC promoter/CAT reporter constructs into primary cultures of cardiac myocytes derived from one day old rat pups. DEX administration into newborn rats produced significant cardiac hypertrophy ranging from 23% at day 1 to 59% at 9 days. The hypertrophy was accompanied by immediate increase (83%) in steady state level of the α-MHC mRNA within one day and a maximum increase (148%) at 7 days of treatment. The steady state level of β-MHC mRNA declined by 25% at day 1 and a maximum decrease of 54% at day 7 of DEX treatment. The changes in MHC mRNA were also reflected in their protein levels as determined by V1 and V3 isozyme analysis. DEX treatment of primary cultures of cardiomyocytes following transfection with a-MHC promoter/CAT reporter constructs resulted in increased CAT expression in a dose dependent manner. The minimum α-MHC gene sequences responding to DEX treatment were located between the -200 to -74-bp region of the gene, resulting in 2-fold and 6-fold activation of CAT reporter after 0.05 and 0.1 mM doses of DEX, respectively. Our data indicate that DEX induced cardiac hypertrophy in newborn rats is accompanied by increased expression of α-MHC and decreased expression of β-MHC. The α-MHC effects are mediated in part through transcriptional mechanisms.
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  • 73
    Digitale Medien
    Digitale Medien
    Springer
    Molecular and cellular biochemistry 212 (2000), S. 5-9 
    ISSN: 1573-4919
    Schlagwort(e): transcriptional regulation ; gene expression ; coactivator ; repressor
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract The CREB-CREM transcription factors are the main gene regulatory effectors of the cAMP signaling pathway. The investigations of this family of transcription factors had a profound impact on the understanding of signaling-induced gene transcription. Here we discuss some key aspects of the underlying biology, review transcriptional activation by CREB proteins through transcription cofactors and present novel insights into the context- and position-specific function of CREB on complex genes.
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  • 74
    ISSN: 1573-4919
    Schlagwort(e): AP-1 ; cobalt chloride ; gene expression ; heme oxygenase ; oxidative stress ; sodium arsenite
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract Previously, chick heme oxygenase-1 (cHO-1) gene was cloned by us and two regions important for induction by sodium arsenite were identified. These two regions were found to contain consensus sequences of an AP-1 (-1580 to -1573) and a MRE/cMyc complex (-52 to -41). In the current study, the roles of these two elements in mediating the sodium arsenite or cobalt chloride dependent induction of cHO-1 were investigated further. DNA binding studies and site-directed mutagenesis studies indicated that both the AP-1 and MRE/cMyc elements are important for the sodium arsenite induction, while cobalt chloride induction involves only the AP-1 element. Electrophoretic mobility shift assays showed that nuclear proteins binding to the AP-1 element was increased by both sodium arsenite or cobalt chloride treatment, whereas the binding of proteins to the MRE/cMyc element showed a high basal expression in untreated cells and the binding activity was only slightly increased by sodium arsenite treatment. Site-directed mutagenesis studies showed that, to completely abolish sodium arsenite induction, both the AP-1 and MRE/cMyc elements must be mutated; mutation of either element alone resulted in only a partial effect. In contrast, a single mutation at AP-1 element was sufficient to reduce the cobalt chloride induction almost completely. The MRE/cMyc complex plays a major role in the basal level expression, and shares some similarities to the upstream stimulatory factor element (USF) identified in the promoter regions of mammalian HO-1 genes and other stress regulated genes. Because sodium arsenite is known to cause oxidative stress and because activation of AP-1 proteins has been shown to be a key step in the oxidative stress response pathway, we also explored the possibility that the induction of the cHO-1 gene by sodium arsenite is mediated through oxidative stress pathway(s) by activation of AP-1 proteins. We found that pretreatment with antioxidants (N-acetyl cysteine or quercetin) reduced the induction of the endogenous cHO-1 message or cHO-1 reporter construct activities induced by sodium arsenite or cobalt chloride. These antioxidants also reduced the protein binding activities to the AP-1 element in the electrophoretic mobility shift assays. In summary, induction of the cHO-1 gene by sodium arsenite or cobalt chloride is mediated by activation of the AP-1 element located at -1,573 to -1,580 of the 5′ UTR.
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  • 75
    Digitale Medien
    Digitale Medien
    Springer
    Molecular and cellular biochemistry 205 (2000), S. 1-11 
    ISSN: 1573-4919
    Schlagwort(e): kidney ; ischemia-reperfusion injury ; free radicals ; reactive oxygen species ; gene expression ; antioxidant enzymes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract Reactive oxygen species (ROS; O2-, H2O2, and OH·), normal by-products of cellular metabolic processes, are kept in control by antioxidant enzymes, such as catalase, glutathione peroxidase (GPX) and superoxide dismutases (SODs). To understand the role of antioxidant enzymatic defenses against ROS injury following ischemia-reperfusion, we examined the effect on kidney exposed to varying periods (30, 60 or 90 min) of ischemia followed by different periods of reperfusion. The enzymatic activities and protein levels of catalase, GPX, CuZnSOD and MnSOD were relatively unaffected at 30 min of ischemia followed by 0, 2 or 24 h reperfusion. However, 60 or 90 min of ischemia followed by 0, 2 or 24 h of reperfusion resulted in a decrease in activities and protein levels which paralleled the duration of ischemic injury. MnSOD activity tended to recover towards normal during reperfusion. Examination of the mRNA levels of these antioxidant enzymes demonstrated a severe decrease in mRNA levels of catalase and GPX at a time point of minimal ischemic injury (30 min of ischemia followed by reperfusion) suggesting that loss of mRNA of catalase and GPX may be the first markers of alterations in cellular redox in ischemia-reperfusion injury. Greater loss of mRNA for catalase, GPX and CuZnSOD were observed following longer periods (60 or 90 min) of ischemia. The mRNA for MnSOD was upregulated at all time points of ischemia-reperfusion injury. Actually, the greater decrease in mRNAs for catalase, GPX and CuZnSOD in the acute phase (within 24 h) subsequently showed a further decrease in these enzyme activities in the subacute phase (72 or 120 h after ischemia). These enzyme activities in the 30 min ischemia group, but not in the 90 min group, already showed tendencies for normalization at 120 h after ischemia. To understand the molecular basis of the loss of mRNA of these antioxidant enzymes during ischemia-reperfusion injury, we examined the rate of transcription by nuclear run-on assays. The similar rates of transcription in control and kidney exposed to ischemia-reperfusion indicates that the loss of mRNA for catalase, GPX and CuZnSOD are possibly due to the increased rate of turnover of their mRNAs. These studies suggest that expression of antioxidant genes during ischemia-reperfusion are not coordinately expressed and the differential loss of antioxidant enzymes may be the contributing factor(s) towards the heterogeneous renal tissue damage as a result of ischemia-reperfusion induced oxidative stress.
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  • 76
    ISSN: 1573-4919
    Schlagwort(e): prostaglandin ; cyclooxygenase ; transcriptional regulation ; gene expression ; promotor activation ; transcription ; endothelial cells
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract Interleukin-1β (IL-1) is a potent inducer of cyclooxygenase-2 (COX-2) and prostaglandin biosynthesis in many types of cells, yet little is known about the molecular mechanisms regulating IL-1 mediated prostanoid biosynthesis in the endothelium of the microvasculature. Therefore, we examined the cis- and trans-acting factors regulating IL-1-induced COX-2 expression in the human microvascular endothelial cell line, HMEC-1. IL-1 enhanced steady state levels of COX-2 protein and mRNA synthesis by ≈ 2-fold which preceded a 2-fold increase in PGFα biosynthesis. Expression of a series of COX-2 promoter-luciferase constructs in IL-1 treated HMEC-1 cells revealed that the 'full length' (-1432/+59 bp) promoter was 10 times more active than the SV-40 promoter/enhancer and that it could be further activated by IL-1. Surprisingly however, all except for the shortest COX-2 promoter construct retained the ability to respond to IL-1 and luciferase activity driven by -191/+59 bp COX-2 promoter was as responsive to IL-1 as the full-length promoter. Moreover, site-directed promoter mutagenesis and electophoretic mobility shift assays (EMSA) indicate that the combinatorial actions of AP2, NF-IL6, and CRE elements are critical for both constitutive and IL-1-inducible COX-2 promoter activity. Understanding the mechanism(s) regulating COX-2 gene expression and prostaglandin biosynthesis in the microvasculature has important implications with regard to inflammation and angiogenesis in vivo.
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  • 77
    Digitale Medien
    Digitale Medien
    Springer
    Molecular and cellular biochemistry 203 (2000), S. 163-167 
    ISSN: 1573-4919
    Schlagwort(e): thymosin β-4 ; gene expression ; chloramphenicol acetyltransferase ; NIH3T3 cells ; interferon response
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract Expression of the gene coding for thymosin β-4 (Tβ-4), the major G-actin sequestering peptide in the cell, is regulated mainly at the level of transcription. In this study, we examined the nucleotide sequence of the 5′-flanking region (from - 2202 to - 881) of the mouse Tβ-4 gene, and demonstrated that the DNA fragment from -278 to +410 of this gene was capable of directing the expression of a chloramphenicol acetyltransferase reporter gene in NIH3T3 cells. However, expression of the reporter gene in cells cannot be induced by interferon-a treatment even though a rapid activation of endogenous Tβ-4 gene by this cytokine was observed. These results suggest that the projected interferon-stimulated response element (ISRE) might reside in other parts of the mouse Tβ-4 gene.
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  • 78
    Digitale Medien
    Digitale Medien
    Springer
    Molecular and cellular biochemistry 211 (2000), S. 103-110 
    ISSN: 1573-4919
    Schlagwort(e): thioacetamide ; glutathione-S-transferase ; rat liver ; transcription ; gene expression
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract The effect of thioacetamide (TA), an hepatotoxic and hepatocarcinogenic compound, on the expression and activity of the cytosolic enzyme glutathione-S-transferase (GST) was studied in rat liver. Four h following the administration of 14C-labeled thioacetamide (10 mg/Kg), several subunits of GST were found to be radioactively labeled. A single sublethal dose of TA (250 mg/Kg) decreased by three-fold the expression of classα GST at 24-48 h of treatment, but did not significantly affect the transcription of class μ GST. The activity of the enzyme toward 1-chloro-2,4-dinitrobenzene was mildly inhibited (66% of the control) by a 24 h TA treatment and gradually increased thereafter. It is proposed that the covalent binding of TA or its derivative to the GST subunits does not affect the activity of the enzyme. Nevertheless, GST activity inhibition is due to the deleterious effect of TA on GST transcription.
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  • 79
    Digitale Medien
    Digitale Medien
    Springer
    Journal of chemical ecology 26 (2000), S. 2015-2023 
    ISSN: 1573-1561
    Schlagwort(e): Allelopathy ; growth ; phenolics ; Hebeloma ; Hymenoscyphus ; Picea abies ; Vaccinium myrtillus
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Allelopathy due to humus phenolics is a cause of natural regeneration deficiency in subalpine Norway spruce (Picea abies) forests. If inhibition of spruce germination and seedling growth due to allelochemicals is generally accepted, in contrast there is a lack of knowledge about phenolic effects on mycorrhizal fungi. Thus, this work tested effects of a humic solution and its naturally occurring phenolics on the growth and respiration of two mycorrhizal fungi: Hymenoscyphus ericae (symbiont of Vaccinium myrtillus, the main allelochemical-producing plant) and Hebeloma crustuliniforme (symbiont of P. abies, the target plant). Growth and respiration of H. crustuliniforme were inhibited by growth medium with the original humic solution (−6% and −30%), respectively, whereas the same humic solution did not affect growth but decreased respiration of H. ericae (−55%). When naturally occurring phenolics (same chemicals and concentrations in the original humic solution) were added to the growth medium, growth of H. crustuliniforme was not affected, whereas that of H. ericae significantly increased (+10%). We conclude that H. ericae is better adapted to the allelopathic constraints of this forest soil than H. crustuliniforme and that the dominance of V. myrtillus among understory species could be explained in this way.
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  • 80
    Digitale Medien
    Digitale Medien
    Springer
    Molecular and cellular biochemistry 209 (2000), S. 125-129 
    ISSN: 1573-4919
    Schlagwort(e): apolipoprotein E ; apolipoprotein A-I ; gene expression ; transgenic mouse
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract The levels of plasma apolipoprotein (apo) E, an anti-atherogenic protein involved in mammalian cholesterol transport, were found to be 2-3 fold lower in mice over-expressing human apoA-I gene. ApoE is mainly associated with VLDL and HDL-size particles, but in mice the majority of the apoE is associated with the HDL particles. Over-expression of the human apoA-I in mice increases the levels of human apoA-I-rich HDL particles by displacing mouse apoA-I from HDL. This results in lowering of plasma levels of mouse apoA-I. Since plasma levels of apoE also decreased in the apoA-I transgenic mice, the mechanism of apoE lowering was investigated. Although plasma levels of apoE decreased by 2-3 fold, apoB levels remained unchanged. As expected, the plasma levels of human apoA-I were almost 5-fold higher in the apoAI-Tg mice compared to mouse apoA-I in WT mice. If the over-expression of human apoA-I caused displacement of apoE from the HDL, the levels of hepatic apoE mRNA should remain the same in WT and the apoAI-Tg mice. However, the measurements of apoE mRNA in the liver showed 3-fold decreases of apoE mRNA in apoAI-Tg mice as compared to WT mice, suggesting that the decreased apoE mRNA expression, but not the displacement of the apoE from HDL, resulted in the lowering of plasma apoE in apoAI-Tg mice. As expected, the levels of hepatic apoA-I mRNA (transgene) were 5-fold higher in the apoAI-Tg mice. ApoE synthesis measured in hepatocytes also showed lower synthesis of apoE in the apoAI-Tg mice. These studies suggest that the integration of human apoA-I transgene in mouse genome occurred at a site that affected apoE gene expression. Identification of this locus may provide further understanding of the apoE gene expression.
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  • 81
    Digitale Medien
    Digitale Medien
    Springer
    Molecular and cellular biochemistry 212 (2000), S. 29-34 
    ISSN: 1573-4919
    Schlagwort(e): cAMP ; transcription factor-decoy oligonucleotides ; CRE ; Ap-1 ; p53 ; tumor growth ; gene expression
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract Nucleic acid molecules with high affinities for a target transcription factor can be introduced into cells as decoy cis-elements to bind these factors and alter gene expression. This review discusses a synthetic single-stranded palindromic oligonucleotide, which self-hybridizes to form a duplex/hairpin and competes with cAMP response element (CRE) enhancers for binding transcription factors. This oligonucleotide inhibits CRE- and Ap-1-directed gene transcription and promotes growth inhibition in vitro and in vivo in a broad spectrum of cancer cells, without adversely affecting normal cell growth. Evidence presented here suggests that the CRE-decoy oligonucleotide can provide a powerful new means of combating cancers, viral diseases, and other pathological conditions by regulating the expression of cAMP-responsive genes.
    Materialart: Digitale Medien
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  • 82
    Digitale Medien
    Digitale Medien
    Springer
    Molecular and cellular biochemistry 212 (2000), S. 73-79 
    ISSN: 1573-4919
    Schlagwort(e): adrenergic receptors ; renin-angiotensin system (RAS) ; gene expression ; kidney
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract To investigate the molecular mechanism(s) of action of catecholamines on the expression of the angiotensinogen (ANG) gene in kidney proximal tubular cells, we used opossum kidney (OK) cells with a fusion gene containing the 5′-flanking regulatory sequence of the rat ANG gene fused with a human growth hormone (hGH) gene as a reporter, pOGH (rANG N-1498/+18), permanently integrated into their genomes. The level of expression of the ANG-GH fusion gene was quantified by the amount of immunoreactive-hGH (IR-hGH) secreted into the medium. The addition of norepinephrine (NE), isoproterenol (a β1/β2-adrenergic receptor (AR) agonist) and iodoclonidine (an α2-AR agonist) stimulated the expression of the ANG-GH fusion gene in a dose-dependent manner, whereas the addition of epinephrine and phenylephrine (α1-AR agonist) had no effect. The stimulatory effect of NE was blocked by the presence of propranolol (β-AR blocker), atenolol (β1-AR blocker), yohimbine (α2-AR blocker), Rp-cAMP (an inhibitor of cAMP-dependent protein kinase AI & AII) and staurosporine (an inhibitor of protein kinase C), but was not blocked by ICI 118, 551 (β2-AR blocker) and prazosin (α1-AR blocker). The addition of a combination of isoproterenol and iodoclonidine or a combination of 8-Bromo-cAMP (8-Br-cAMP) and phorbol 12-myristate (PMA) synergistically stimulated the expression of the ANG-GH fusion gene as compared to the addition of isoproterenol, iodoclonidine, 8-Br-cAMP or PMA alone. Furthermore, the addition of NE, 8-Br-cAMP or PMA stimulated the expression of pOGH (rANG N-806/-779/-53/+18), a fusion gene containing the putative cAMP responsive element (CRE, ANG N-806/-779) upstream of the ANG promoter (ANG N-53/+18) in OK cells, but had no effect on the expression of fusion genes containing the mutant of the CRE. Gel mobility shift assays revealed that the ANG-CRE binds with the DNA-binding domain (bZIP 254-327) of the cAMP-responsive binding protein (CREB). The binding of the labeled ANG-CRE to CREB (bZIP254-327) was displaced by unlabeled ANG-CRE and the CRE of the somatostatin gene but not by the mutants of the ANG-CRE. Finally, NE stimulated the phosphorylation of CREB in OK cells. These studies demonstrate that the molecular mechanism(s) of NE action on the expression of the ANG gene in OK cells may be mediated via both the PKA and PKC signalling pathways and via the phosphorylation of CREB. The phosphorylated CREB then interacts with the CRE in the 5′-flanking region of the ANG gene and subsequently stimulates the gene expression.
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  • 83
    Digitale Medien
    Digitale Medien
    Springer
    Molecular and cellular biochemistry 212 (2000), S. 135-142 
    ISSN: 1573-4919
    Schlagwort(e): gene expression ; catecholamines ; angiotensin II ; heart failure ; myosin ; hypertension ; eprosartan
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract Pressure overload of the heart is associated with a perturbed gene expression of the cardiomyocyte leading to an impaired pump function. The ensuing neuro-endocrine activation results in disordered influences of angiotensin II and catecholamines on gene expression. To assess whether angiotensin II type 1 receptor inhibition can also counteract a raised sympathetic nervous system activity, spontaneously hypertensive rats fed a hypercaloric diet were treated with eprosartan (daily 90 mg/kg body wt) and cardiovascular parameters were monitored with implanted radiotelemetry pressure transducers. Both, blood pressure and heart rate were increased (p 〈 0.05) by the hypercaloric diet. Although eprosartan reduced (p 〈 0.05) the raised systolic and diastolic blood pressure, the diet-induced rise in heart rate was blunted only partially. In addition to drugs interfering with the enhanced catecholamine influence, compounds should be considered that selectively affect cardiomyocyte gene expression via 'metabolic' signals.
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  • 84
    ISSN: 1573-4919
    Schlagwort(e): angiotensinogen ; fibronectin ; gene expression ; transcriptional regulation ; cardiomyocytes ; vascular smooth muscle cells
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract Angiotensinogen (AGT) is a unique substrate of the renin-angiotensin system and fibronectin (FN) is an important component of the extracellular matrix. These play critical roles in the pathophysiological changes including cardiovascular remodeling and hypertrophy in response to hypertension. This study was performed to examine the regulation of AGT and FN gene in cardiac myocytes (CMs) and vascular smooth muscle cells (VSMCs) in response to mechanical stretch. Mechanical stretch significantly increased the AGT mRNA expression in CMs, while these stimuli did not affect FN mRNA levels. On the other hand, Mechanical stretch upregulated FN mRNA levels in VSMCs, whereas no increase in AGT mRNA levels was observed in response to stretch stimuli. An angiotensin II type 1 (AT1) receptor antagonist (CV11974) significantly decreased these stretch-mediated increases in mRNA level and promoter activity of the AGT and FN gene, whereas angiotensin II type 2 (AT2) receptor antagonist (PD123319) did not affect the induction. These results indicate that mechanical stretch activates transcription of the AGT and FN gene mainly via AT1 receptor-pathway in CMs and VSMCs. Furthermore, mechanisms regulating AGT and FN gene seem to be different between CMs and VSMCs.
    Materialart: Digitale Medien
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  • 85
    Digitale Medien
    Digitale Medien
    Springer
    Molecular and cellular biochemistry 212 (2000), S. 211-217 
    ISSN: 1573-4919
    Schlagwort(e): angiotensin receptor ; medullary thick ascending limb ; sodium intake ; primary cell culture ; gene expression
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract Angiotensin II (Ang II) is an important regulator of the function of medullary thick ascending limb of loop of Henle (MTAL). Recent studies showed that changes in Ang II receptor expression occur and underlie changes in the function of proximal tubules during altered sodium intake. The present experiment was designed to determine (1) whether expression of the type 1 Ang II (AT1) receptor in the MTAL is regulated by altered sodium intake, and (2) the specific pathway(s) mediating sodium-induced AT1 expression in the MTAL. Wistar rats were fed a normal sodium (0.5%, NS), low sodium (0.07%, LS), or high sodium (4%, HS) diet for 2 weeks. Northern blot analysis and radioligand binding showed that in rats fed a normal sodium diet the rank of order for both AT1 mRNA expression and receptor density was outer medulla 〉 cortex 〉 inner medulla. Sodium restriction significantly increased both AT1 mRNA expression and receptor density in the outer medulla. In contrast, neither AT1 mRNA expression nor receptor density in the outer medulla was altered by sodium loading. Losartan treatment (3 mg/kg/per day by oral gavage for 2 weeks) prevented low sodium-induced upregulation of the AT1 receptor in the outer medulla, but it had no effect on AT1 expression in the outer medulla of rats fed a normal sodium diet. Highly purified suspensions of MTAL were isolated from rats fed a normal or low sodium diet. Low sodium intake significantly increased AT1 mRNA level by 184% and AT1 receptor density by 58% in MTALs. Primary cultures of MTAL cells were treated with PBS, Ang II (10-8 M), and Ang II + 17 octadecynoic (17 ODYA, 10 μM). Ang II caused about 2-fold increase in AT1 mRNA levels, and this increase was diminished by about 30% by the addition of 17 ODYA. We conclude that (1) sodium restriction but not sodium loading increases AT1 receptor expression in the MTAL, (2) low sodium-induced upregulation of the AT1 receptor in the MTAL is Ang II-dependent, and (3) Ang II-induced upregulation of the AT1 receptor in the MTAL is mediated, at least in part, by cytochrome P450 pathways.
    Materialart: Digitale Medien
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  • 86
    ISSN: 1573-4919
    Schlagwort(e): renin angiotensin system ; sarcoplasmic reticulum ; Ca2+-handling ; gene expression ; ischemia-reperfusion ; cardioprotection
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract The aim of this study was to explore the possible participation of cardiac renin-angiotensin system (RAS) in the ischemia-reperfusion induced changes in heart function as well as Ca2+-handling activities and gene expression of cardiac sarcoplasmic reticulum (SR) proteins. The isolated rat hearts, treated for 10 min without and with 30 μM captopril or 100 μM losartan, were subjected to 30 min ischemia followed by reperfusion for 60 min and processed for the measurement of SR function and gene expression. Attenuated recovery of the left ventricular developed pressure (LVDP) upon reperfusion of the ischemic heart was accompanied by a marked reduction in SR Ca2+-pump ATPase, Ca2+-uptake and Ca2+-release activities. Northern blot analysis revealed that mRNA levels for SR Ca2+-handling proteins such as Ca2+-pump ATPase (SERCA2a), ryanodine receptor, calsequestrin and phospholamban were decreased in the ischemia-reperfused heart as compared with the non-ischemic control. Treatment with captopril improved the recovery of LVDP as well as SR Ca2+-pump ATPase and Ca2+-uptake activities in the postischemic hearts but had no effect on changes in Ca2+-release activity due to ischemic-reperfusion. Losartan neither affected the changes in contractile function nor modified alterations in SR Ca2+-handling activities. The ischemia-reperfusion induced decrease in mRNA levels for SR Ca2+-handling proteins were not affected by treatment with captopril or losartan. The results suggest that the improvement of cardiac function in the ischemic-reperfused heart by captopril is associated with the preservation of SR Ca2+-pump activities; however, it is unlikely that this action of captopril is mediated through the modification of cardiac RAS. Furthermore, cardiac RAS does not appear to contribute towards the ischemia-reperfusion induced changes in gene expression for SR Ca2+-handling proteins.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 87
    ISSN: 1573-4919
    Schlagwort(e): pressure overload ; gene expression ; subcellular remodeling ; sarcoplasmic reticulum Ca2+-handling ; anti-hypertensive therapy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract The effects of propranolol and verapamil on contractile dysfunction, subcellular remodeling and changes in gene expression in cardiac hypertrophy due to pressure overload were examined. Rats were subjected to banding of the abdominal aorta and then treated with either propranolol (10 mg/kg daily), verapamil (5 mg/kg daily) or vehicle for 8 weeks after the surgery. Depression of the left ventricular function in the hypertrophied heart was associated with decreases in myofibrillar and myosin CA2+ ATPase activities as well as Ca2+-pump and Ca2+-release activities of the sarcoplasmic reticulum (SR). The level of a-myosin heavy chain (α-MHC) mRNA was decreased while that of β-MHC mRNA was increased in the pressure-overloaded heart. The level of SR Ca2+-pump ATPase (SERCA2) mRNA and protein content for SERCA2 were decreased in the pressure overloaded heart. Treatment of the hypertrophied animals with propranolol or verapamil resulted in preservation of the left ventricular function and prevention of the subcellular alterations. Shift in the α- and β-MHC mRNA levels and changes in the expression in SERCA2 mRNA level and protein content were also attenuated by these treatments. The results suggest that blockade of β-adrenoceptors or voltage-dependent calcium channels normalizes the cardiac gene expression, prevents subcellular remodeling and thus attenuates heart dysfunction in rats with cardiac hypertrophy. Furthermore, both cardiac β-adrenoceptors and L-type Ca2+-channels may be involved in the genesis of cardiac hypertrophy due to pressure overload.
    Materialart: Digitale Medien
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  • 88
    Digitale Medien
    Digitale Medien
    Springer
    Molecular and cellular biochemistry 213 (2000), S. 119-126 
    ISSN: 1573-4919
    Schlagwort(e): TIS11 ; an immediate early gene ; gene cloning ; gene expression ; gene organization ; promoter
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Abstract The TIS11 gene is an immediate early gene that is induced rapidly and transiently by phorbol 12-myristate 13-acetate and various growth factors. To study transcriptional regulation of the gene, a genomic clone of rat TIS11 was isolated, and the organization of exon-intron structure and transcriptional initiation site were determined. The rat TIS11 gene consisted of 2 exons spanning approximately 2.5 kb. Several canonical sequences for binding of transcriptional factors were found in the 5′-flanking region. The 5.3 kb of the 5′-flanking region fused to a luciferase reporter gene showed promoter activity when introduced into rat pheochromocytoma PC12 cells. Analyses with serial 5′-deletion mutants suggested that the major positive regulatory region is located at the region of -241 to -76, and that the minimum promoter region is within the 76-bp upstream of the transcriptional initiation site. Gel mobility shift assays revealed that PC12 cell nuclear proteins specifically bind to the major positive regulatory region of the TIS11 gene. The identified nuclear protein components may act as the positive trans-acting factors in the basal expression of the TIS11 gene in PC12 cells.
    Materialart: Digitale Medien
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  • 89
    ISSN: 1432-2242
    Schlagwort(e): Key words Pinus thunbergii ; Pine needle gall midge ; RAPD ; Bulked segregant analysis ; Linkage
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Linkage of RAPD markers to a single dominant gene for resistance to pine needle gall midge was investigated in Japanese black pine (Pinus thunbergii). Three primers that generated linked markers were found after 1160 primers were screened by bulked segregant analysis. The distances between the resistance gene, R, and the marker genes OPC06580, OPD01700, and OPAX192100 were 5.1 cM, 6.7 cM and 13.6 cM, respectively. OPC06580 was in coupling phase to R, whereas OPD01700 and OPAX192100 were in repulsion phase to R. A linkage map for a resistant tree was constructed using 96 macrogametophytes. In linkage analysis, 98 out of 127 polymorphic markers were assigned to 17 linkage groups and six linked pairs. The total length of this map was 1469.8 cM, with an average marker density of 15.6 cM. The genome length was estimated to be 2138.3 cM, and the derived linkage map covered 67.5% of the genome. Although the linked markers OPC06580, OPAX192100, and OPD01700, belonged to the same linkage group, no precise positions were found for OPC06580 or OPD01700.
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  • 90
    Digitale Medien
    Digitale Medien
    Springer
    Theoretical and applied genetics 101 (2000), S. 1250-1258 
    ISSN: 1432-2242
    Schlagwort(e): Key words Micronuclei ; Microprotoplasts ; Chromosome transfer ; RAPD ; Helianthus
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract  Asymmetric somatic hybrid (ASH) plants were obtained by PEG-mediated mass fusion of microprotoplasts from perennial Helianthus species and hypocotyl protoplasts of Helianthus annuus. The formation of micronuclei in perennial sunflower cell cultures was induced, at early log phase, by addition of the herbicides amiprophos-methyl or oryzalin. Sub-diploid microprotoplasts were isolated by high-speed centrifugation and the smallest enriched by sequential filtration through nylon sieves of decreasing pore size. Fusion products were cultured and the regenerated plants phenotypically, genetically and cytologically characterized. DNA analysis using RAPD markers revealed that 28 out of 53 regenerated plants were asymmetric hybrids. Subsequent nuclear-DNA flow cytometric analysis showed that these plants had a higher DNA content than the receptor H. annuus, suggesting that they represented addition lines. Cytological investigation of the metaphase cells of 16 hybrids revealed an addition of 2–8 extra chromosomes in these plants. The phenotype of most ASH plants resembled H. annuus. These results indicate that micronuclear induction and asymmetric somatic hybridization represent a potent tool for partial genome transfer aimed at the specific transfer of economically important traits in breeding programs.
    Materialart: Digitale Medien
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  • 91
    ISSN: 1871-4528
    Schlagwort(e): somatic hybrids ; potato leafroll virus ; RAPD ; Solanum wild species ; pollen fertility
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Somatic fusions between an accession of the diploid wild speciesSolanum verrucosum and a dihaploid S.tuberosum genotype were produced in order to incorporate resistance to potato leafroll virus (PLRV). In total 15 somatic hybrids out of 16 regenerants were obtained. Identification of hybrids was based on additive RAPD patterns, general morphological characteristics, chromosome numbers and chloroplast counts in stomata guard cells. A field trial was performed with the hybrids, their two parents and the control cultivar Kennebec to assess field performance and phenotypic variability. Yield parameters varied considerably among somatic hybrids. Some of the hybrids gave significantly higher yields, tuber numbers and tuber weights than both parents. Pollen fertility of hybrids ranged from 19 to 77%. Twelve hybrids were found to be resistant to PLRV.
    Materialart: Digitale Medien
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  • 92
    Digitale Medien
    Digitale Medien
    Springer
    Potato research 43 (2000), S. 383-393 
    ISSN: 1871-4528
    Schlagwort(e): Solanum tuberosum L. ; plant development ; antioxidant genes ; gene expression
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary The expression of antioxidant genes has been analyzed in a potato plant and during tuber dormancy. Manganese superoxide dismutase (MnSOD), cytosolic copper and zinc superoide dismutase (Cu/ZnSOD), catalase class II, cytosolic ascorbate peroxidase (APX) and glutathione peroxidase (GPX) are expressed at the RNA level in all the contexts analyzed. By contrast, the expression of the iron superoxide dismutase (FeSOD) and plastidic Cu/ZnSOD seems to be limited to green tissues, as shown by northern blots and native gels. A complex DAB-peroxidase isozyme pattern (using diaminobenzidine as substrate) has been observed in different developmental contexts analyzed, but hardly observed in tubers. During tuber dormancy, MnSOD and cytosolic Cu/ZnSOD activity was relatively constant in both Désirée and Bintje varieties while catalase activity decreases. Moreover, tuber dormancy breakage did not involve significant changes in the activity of these enzymes. On the basis of these results, the possible link between active oxygen species (AOS) metabolism and dormancy is discussed.
    Materialart: Digitale Medien
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  • 93
    ISSN: 1871-4528
    Schlagwort(e): gene expression ; cDNA-AFLP ; RNA-fingerprinting ; organogenesis ; tuberisation ; dormancy ; sprouting ; cluster analysis ; metabolic pathways
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Potato tuber life-cycle is composed of many individual developmental stages including tuber formation, tuber development, dormancy and sprouting. We have used cDNA-AFLP fingerprinting to analyse gene expression in 24 individual stages of development, over the period from stolon formation through sprouting. In addition to these developmental stages, different tissues were analysed to assess tissue specificity and various controls were incorporated to determine process specificity. In total around 18000 transcript derived cDNA fragments (TDFs) were visualised from which circa 2600 were included in a statistical analysis allowing general conclusions about gene expression during development. More than 200 process specific TDFs were isolated and sequenced throughout the potato tuber life-cycle. The sequence similarities of these TDFs to known genes give an insight into the kinds of processes occurring during tuberisation, dormancy and sprouting.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 94
    ISSN: 1611-4663
    Schlagwort(e): Lentinula edodes ; Heterozygous DNA marker ; RAPD ; de-dikaryotization ; Protoplast
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft , Maschinenbau
    Notizen: Abstract A suitable screening method for heterozygous DNA markers in shiitake,Lentinula edodes (Berk.) Pegler, is reported. Monokaryons were derived from a dikaryon by de-dikaryotization via protoplast formation. Compatibility of the monokaryons was determined by pairwise culture on agar plates. We selected the primers to amplify polymorphic fragments among the original strain (Hokken600∶H600) and two monokaryons (H600PP-39 and H600PP-67) showing compatibility. A total of 135 fragments were selected as specific random amplified polymorphic DNAs (RAPDs) resulting from 56 primers of the 147 primers tested. Furthermore, we tested whether the polymorphic fragments segregated into 2∶2 among four strains isolated from a basidium. Most of the polymorphic fragments (about 97.8%) showed 2∶2 segregation among the four strains. We concluded that the polymorphic fragments were heterozygous if they were detected in either of the monokaryons (H600PP-39 and H600PP-67) and segregated to 2∶2 among four meiotic strains (H600B-1,-2, -3, and -4). A total of 132 heterozygous DNA markers were therefore selected from a dikaryon of shiitake (Hokken600∶H600).
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  • 95
    Digitale Medien
    Digitale Medien
    Springer
    Mycoscience 41 (2000), S. 145-148 
    ISSN: 1618-2545
    Schlagwort(e): mycelial compatibility ; RAPD ; Sclerotium rolfsii
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Sclerotium rolfsii isolates from peanut fields in Ibaraki were classified into mycelial compatibility groups (MCGs) based on the barrage zone formation. A total of 132 isolates collected from four fields within a 120 m radius in 1994 comprised four MCGs; MCG A (71 isolates), B (34 isolates), C (26 isolates) and D (one isolate). Fields 1 and 2 were occupied exclusively by MCG A. MCG A also predominated in field 3. In field 4, MCGs A, B and C were dominant. Population structure in 3 additional fields was determined in 1997. All 11 isolates from Field 5, which was 400 m distant from field 1, belonged to MCG C. A total of 42 isolates from fields 6 and 7, 2.5 km distant from other fields and 100 m distant from each other, were all MCG A. These results suggested that the population structure ofS. rolfsii was simple. RAPD fingerprintings showed that most isolates of the same MCG were clonal.
    Materialart: Digitale Medien
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  • 96
    ISSN: 1608-3237
    Schlagwort(e): poly-β-hydroxybutyric acid ; Yersinia pseudotuberculosis ; Listeria monocytogenes ; temperature ; growth
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A comparative investigation of the intracellular content of poly-β-hydroxybutyric acid showed that Yersinia pseudotuberculosis strains accumulated, on the average, lower amounts of this reserve substance than Listeria monocytogenes strains. The intracellular pool of poly-β-hydroxybutyric acid was responsible for the growth of the bacteria at low temperatures (4–6°C) in the absence of any exogenous carbon and energy source.
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  • 97
    ISSN: 1608-3407
    Schlagwort(e): Scenedesmus quadricauda ; growth ; cell size ; photosynthetic activity ; imazalil sulfate ; three-phase dose response
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Three-phase dose responses of biological systems of different levels of organization are often called “paradoxical” because the biological effects are clearly manifested under low- and high-intensity treatments, but are absent during moderate-strength treatments. In this work, we found anomalous changes in the cell number of a green alga Scenedesmus quadricauda (Turp.) Breb. grown in the presence of the fungicide imazalil sulfate. At low imazalil concentrations (2.5 × 10–9–2.5 × 10–6 M), the slow increase in the cell number as compared to an untreated culture was not related to cell death. As seen by the dynamics of the population structure and cell functional characteristics (photosynthesis, thermal stability of photosynthetic membranes, etc.), the decrease in the growth rate at low concentrations of imazalil (2–10 × 10–9 M) was due to a long-term arrest of cell division in a fraction of the cell population rather than to a decrease in the rate of division. The absence of a toxic effect or even a slight stimulation of culture growth at moderate concentrations (0.05–1.25 × 10–6 M) was due to the resumption of cell division after a temporal cessation. At these concentrations, imazalil induced cell stress and adaptive elevation of cell tolerance to the fungicide (acclimation). Cell death was observed only at a high fungicide content in the medium (6.25 × 10–6 and higher). Thus, the three-phase (bimodal) dose response corresponds to two regimes (steady-states) of cell functioning which differ in cell sensitivity to external stimuli. The low-sensitivity state, which is characteristic of cells that have experienced stress, is likely to be the state known as “hormesis.”
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  • 98
    Digitale Medien
    Digitale Medien
    Springer
    Journal of applied phycology 12 (2000), S. 185-189 
    ISSN: 1573-5176
    Schlagwort(e): blue-green alga ; cyanobacterium ; carbon dioxide ; culture ; growth ; Nostocflagelliforme ; rehydration, watering
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The terrestrial blue-green alga (cyanobacterium), Nostoc flagelliforme, was cultured in air at variouslevels of CO2, light and watering to see theireffects on its growth. The alga showed the highestrelative growth rate at the conditions of highCO2 (1500 ppm), high light regime (219–414μmol m-2s-1) and twice daily watering,but the lowest rate at the conditions of low light(58–114 μmol m-2s-1) and daily twicewatering. Increased watering had little effect ongrowth rate at 350 ppm CO2, but increased byabout 70% at 1500ppm CO2 under high lightconditions. It was concluded that enriched CO2could enhance the growth of N. flagelliformewhen sufficient light and water was supplied.
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  • 99
    Digitale Medien
    Digitale Medien
    Springer
    Aquatic ecology 34 (2000), S. 19-27 
    ISSN: 1573-5125
    Schlagwort(e): adaptation ; fitness ; food quality ; growth ; reproduction ; temporary ponds
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Temporary pond Daphnia flourish on relatively poor-quality food, suggesting adaptation to stringent temporary pond conditions. We conducted laboratory life history experiments on populations of Daphnia obtusa from a shaded woodland temporary pond (short hydroperiod, dystrophic) and an open farmland temporary pond (long hydroperiod, eutrophic), and compared a suite of physical/chemical conditions in the ponds to evaluate habitat conditions. We hypothesized that the shaded woodland pond population would be more fit in terms of life history variables for individuals (age and size at first reproduction, mean brood size, mean number of neonates) and populations (generation time, net reproductive rate R 0, and intrinsic reproductive rate r) given a standard, low-quality food (trout chow and yeast). Life history traits of woodland pond animals were mixed, relative to farm pond animals, and consistent with bet-hedging for an unpredictable habitat. Values of life history traits rivaled or exceeded those of other studies using phytoplankton as food, and were influenced by the pond water used for our study. Life histories clearly differ among local and regional temporary pond Daphnia obtusa populations, and should be valuable for examining the relative influences of local selection and metapopulation dynamics on population structure.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 100
    Digitale Medien
    Digitale Medien
    Springer
    Journal of applied phycology 12 (2000), S. 535-542 
    ISSN: 1573-5176
    Schlagwort(e): desiccation ; growth ; growth model ; inorganic carbon ; nutrients ; photoperiod ; photosynthesis ; pigments ; Porphyra linearis ; PPF ; respiration ; temperature ; water velocity
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The effect of environmental parameters on the growthof Porphyra linearis gametophytes was examinedunder controlled conditions, and related to themultilinear regression growth model recently developedfor this seaweed under coastal conditions in theeastern Mediterranean. Growth chambers, a gradienttable, special culture devices and analytical methodswere combined for this culture study.The major factors significantly controlling thegrowth rate of the P. linearis gametophytein glass dishes were: photoperiod, temperature, agein culture, photosynthetic photon flux (PPF), salinityand water dynamics. Maximal growth occurred underdaylength of 12 h, medium temperature (15–20 °C), low PPF (70–140 μmol photon m-2s-1), ambient salinity (30–40 ppt), 1–3 h ofdaily air exposure, and water velocity of 4 cm s-1.Photosynthesis and respiration rates weredominantly affected by daylength and temperature,while the concentration of pigments was dominantlyaffected by PPF and temperature.These conditions correspond well to the optimalnatural growth environment of this local species andare in agreement with the optimum estimated throughthe recently developed outdoor mathematical growthmodel.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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