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  • Superoxide  (3)
  • Temperature  (3)
  • Frontiers Media  (6)
  • American Chemical Society
  • Blackwell Publishing Ltd
  • Wiley
  • 2015-2019  (6)
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  • 1
    Publication Date: 2022-05-25
    Description: © The Author(s), 2017. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Frontiers in Marine Science 4 (2017): 337, doi:10.3389/fmars.2017.00337.
    Description: The fishery for American lobster is currently the highest-valued commercial fishery in the United States, worth over US$620 million in dockside value in 2015. During a marine heat wave in 2012, the fishery was disrupted by the early warming of spring ocean temperatures and subsequent influx of lobster landings. This situation resulted in a price collapse, as the supply chain was not prepared for the early and abundant landings of lobsters. Motivated by this series of events, we have developed a forecast of when the Maine (USA) lobster fishery will shift into its high volume summer landings period. The forecast uses a regression approach to relate spring ocean temperatures derived from four NERACOOS buoys along the coast of Maine to the start day of the high landings period of the fishery. Tested against conditions in past years, the forecast is able to predict the start day to within 1 week of the actual start, and the forecast can be issued 3–4 months prior to the onset of the high-landings period, providing valuable lead-time for the fishery and its associated supply chain to prepare for the upcoming season. Forecast results are conveyed in a probabilistic manner and are updated weekly over a 6-week forecasting period so that users can assess the certainty and consistency of the forecast and factor the uncertainty into their use of the information in a given year. By focusing on the timing of events, this type of seasonal forecast provides climate-relevant information to users at time scales that are meaningful for operational decisions. As climate change alters seasonal phenology and reduces the reliability of past experience as a guide for future expectations, this type of forecast can enable fishing industry participants to better adjust to and prepare for operating in the context of climate change.
    Description: This forecast was initiated with support from NSF Coastal SEES (OCE 1325484) and was developed with funds from NASA EPSCoR through Maine Space Grant Consortium (EP-15-03).
    Keywords: Seasonal forecast ; Temperature ; Fishery landings ; Lobster fishery ; Climate variability
    Repository Name: Woods Hole Open Access Server
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  • 2
    Publication Date: 2022-05-25
    Description: © The Author(s), 2016. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Frontiers in Chemistry 4 (2016): 5, doi:10.3389/fchem.2016.00005.
    Description: Biological production and decay of the reactive oxygen species (ROS) hydrogen peroxide (H2O2) and superoxide (O−2) likely have significant effects on the cycling of trace metals and carbon in marine systems. In this study, extracellular production rates of H2O2 and O−2 were determined for five species of marine diatoms in the presence and absence of light. Production of both ROS was measured in parallel by suspending cells on filters and measuring the ROS downstream using chemiluminescence probes. In addition, the ability of these organisms to break down O−2 and H2O2 was examined by measuring recovery of O−2 and H2O2 added to the influent medium. O−2 production rates ranged from undetectable to 7.3 × 10−16 mol cell−1 h−1, while H2O2 production rates ranged from undetectable to 3.4 × 10−16 mol cell−1 h−1. Results suggest that extracellular ROS production occurs through a variety of pathways even amongst organisms of the same genus. Thalassiosira spp. produced more O−2 in light than dark, even when the organisms were killed, indicating that O−2 is produced via a passive photochemical process on the cell surface. The ratio of H2O2 to O−2 production rates was consistent with production of H2O2 solely through dismutation of O−2 for T. oceanica, while T. pseudonana made much more H2O2 than O−2. T. weissflogii only produced H2O2 when stressed or killed. P. tricornutum cells did not make cell-associated ROS, but did secrete H2O2-producing substances into the growth medium. In all organisms, recovery rates for killed cultures (94–100% H2O2; 10–80% O−2) were consistently higher than those for live cultures (65–95% H2O2; 10–50% O−2). While recovery rates for killed cultures in H2O2 indicate that nearly all H2O2 was degraded by active cell processes, O−2 decay appeared to occur via a combination of active and passive processes. Overall, this study shows that the rates and pathways for ROS production and decay vary greatly among diatom species, even between those that are closely related, and as a function of light conditions.
    Description: This research was supported by NSF grant OCE-1131734/1246174 to BV and CH.
    Keywords: Reactive oxygen species ; Superoxide ; Hydrogen peroxide ; Diatoms ; Culture
    Repository Name: Woods Hole Open Access Server
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  • 3
    Publication Date: 2022-10-26
    Description: © The Author(s), 2019. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Plummeer, S., Taylor, A. E., Harvey, E. L., Hansel, C. M., & Diaz, J. M. Dynamic regulation of extracellular superoxide production by the coccolithophore Emiliania huxleyi (CCMP 374). Frontiers in Microbiology, 10, (2019): 1546, doi: 10.3389/fmicb.2019.01546.
    Description: In marine waters, ubiquitous reactive oxygen species (ROS) drive biogeochemical cycling of metals and carbon. Marine phytoplankton produce the ROS superoxide (O2−) extracellularly and can be a dominant source of O2− in natural aquatic systems. However, the cellular regulation, biological functioning, and broader ecological impacts of extracellular O2− production by marine phytoplankton remain mysterious. Here, we explored the regulation and potential roles of extracellular O2− production by a noncalcifying strain of the cosmopolitan coccolithophorid Emiliania huxleyi, a key species of marine phytoplankton that has not been examined for extracellular O2− production previously. Cell-normalized extracellular O2− production was the highest under presumably low-stress conditions during active proliferation and inversely related to cell density during exponential growth phase. Removal of extracellular O2− through addition of the O2− scavenger superoxide dismutase (SOD), however, increased growth rates, growth yields, cell biovolume, and photosynthetic efficiency (Fv/Fm) indicating an overall physiological improvement. Thus, the presence of extracellular O2− does not directly stimulate E. huxleyi proliferation, as previously suggested for other phytoplankton, bacteria, fungi, and protists. Extracellular O2− production decreased in the dark, suggesting a connection with photosynthetic processes. Taken together, the tight regulation of this stress independent production of extracellular O2− by E. huxleyi suggests that it could be involved in fundamental photophysiological processes.
    Description: This research was supported by a Junior Faculty Seed Grant from the University of Georgia Research Foundation (JD), a National Science Foundation (NSF) Graduate Research Fellowship (SP), and NSF grant OCE-1355720 (CH). The FlowCam® and FIRe were purchased through a NSF Equipment Improvement Grant (1624593).
    Keywords: Reactive oxygen species ; Superoxide ; Emiliania huxleyi ; Photophysiology ; Oxidative stress ; Redox homeostasis ; Biogeochemical cycling
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  • 4
    Publication Date: 2022-10-26
    Description: © The Author(s), 2019. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Palmer, M. D., Durack, P. J., Paz Chidichimo, M., Church, J. A., Cravatte, S., Hill, K., Johannessen, J. A., Karstensen, J., Lee, T., Legler, D., Mazloff, M., Oka, E., Purkey, S., Rabe, B., Sallee, J., Sloyan, B. M., Speich, S., von Schuckmann, K., Willis, J., & Wijffels, S. Adequacy of the ocean observation system for quantifying regional heat and freshwater storage and change. Frontiers in Marine Science, 6, (2019): 16, doi: 10.3389/fmars.2019.00416.
    Description: Considerable advances in the global ocean observing system over the last two decades offers an opportunity to provide more quantitative information on changes in heat and freshwater storage. Variations in these storage terms can arise through internal variability and also the response of the ocean to anthropogenic climate change. Disentangling these competing influences on the regional patterns of change and elucidating their governing processes remains an outstanding scientific challenge. This challenge is compounded by instrumental and sampling uncertainties. The combined use of ocean observations and model simulations is the most viable method to assess the forced signal from noise and ascertain the primary drivers of variability and change. Moreover, this approach offers the potential for improved seasonal-to-decadal predictions and the possibility to develop powerful multi-variate constraints on climate model future projections. Regional heat storage changes dominate the steric contribution to sea level rise over most of the ocean and are vital to understanding both global and regional heat budgets. Variations in regional freshwater storage are particularly relevant to our understanding of changes in the hydrological cycle and can potentially be used to verify local ocean mass addition from terrestrial and cryospheric systems associated with contemporary sea level rise. This White Paper will examine the ability of the current ocean observing system to quantify changes in regional heat and freshwater storage. In particular we will seek to answer the question: What time and space scales are currently resolved in different regions of the global oceans? In light of some of the key scientific questions, we will discuss the requirements for measurement accuracy, sampling, and coverage as well as the synergies that can be leveraged by more comprehensively analyzing the multi-variable arrays provided by the integrated observing system.
    Description: MP was supported by the Met Office Hadley Centre Climate Programme funded by the BEIS and Defra, and the European Union’s Horizon 2020 Research and Innovation Program under grant Agreement No. 633211 (AtlantOS). The work of PD was prepared the by Lawrence Livermore National Laboratory (LLNL) under Contract DE-AC52-07NA27344 and is a contribution to the U.S. Department of Energy, Office of Science, Climate and Environmental Sciences Division, Regional and Global Modeling and Analysis Program. LLNL Release number: LLNL-JRNL-761158. BS and JC was partially supported by the Centre for Southern Hemisphere Oceans Research, a joint research center between the QNLM and the CSIRO. BS was also supported by the Australian Government Department of the Environment and CSIRO through the National Environmental Science Program. SC was supported by the IRD and by the French national program LEFE/INSU. SC thanks W. Kessler for suggestions concerning Figure 6. BR was supported by the German Alfred-Wegener-Institut Helmholtz-Zentrum für Polar-und Meeresforschung (AWI). J-BS was supported by the CNRS/INSU and the Horizon 2020 Research and Innovation Program under Grant Agreement 637770. SS was supported by the French Institutions ENS, LMD, IPSL, and CNRS/INSU. The work of JW was performed in part at the Jet Propulsion Laboratory, California Institute of Technology, under contract with the National Aeronautics and Space Administration.
    Keywords: Heat content ; Freshwater content ; Salinity ; Temperature ; Ocean observing system ; Climate change ; Climate variability ; Observing system design
    Repository Name: Woods Hole Open Access Server
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  • 5
    Publication Date: 2022-05-26
    Description: © The Author(s), 2016. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Frontiers in Marine Science 3 (2016): 232, doi:10.3389/fmars.2016.00232.
    Description: The reactive oxygen species (ROS) superoxide has been implicated in both beneficial and detrimental processes in coral biology, ranging from pathogenic disease resistance to coral bleaching. Despite the critical role of ROS in coral health, there is a distinct lack of ROS measurements and thus an incomplete understanding of underpinning ROS sources and production mechanisms within coral systems. Here, we quantified in situ extracellular superoxide concentrations at the surfaces of aquaria-hosted Porites astreoides during a diel cycle. High concentrations of superoxide (~10's of nM) were present at coral surfaces, and these levels did not change significantly as a function of time of day. These results indicate that the coral holobiont produces extracellular superoxide in the dark, independent of photosynthesis. As a short-lived anion at physiological pH, superoxide has a limited ability to cross intact biological membranes. Further, removing surface mucus layers from the P. astreoides colonies did not impact external superoxide concentrations. We therefore attribute external superoxide derived from the coral holobiont under these conditions to the activity of the coral host epithelium, rather than mucus-derived epibionts or internal sources such as endosymbionts (e.g., Symbiodinium). However, endosymbionts likely contribute to internal ROS levels via extracellular superoxide production. Indeed, common coral symbionts, including multiple strains of Symbiodinium (clades A to D) and the bacterium Endozoicomonas montiporae LMG 24815, produced extracellular superoxide in the dark and at low light levels. Further, representative P. astreoides symbionts, Symbiodinium CCMP2456 (clade A) and E. montiporae, produced similar concentrations of superoxide alone and in combination with each other, in the dark and low light, and regardless of time of day. Overall, these results indicate that healthy, non-stressed P. astreoides and representative symbionts produce superoxide externally, which is decoupled from photosynthetic activity and circadian control. Corals may therefore produce extracellular superoxide constitutively, highlighting an unclear yet potentially beneficial role for superoxide in coral physiology and health.
    Description: This work was supported by a Postdoctoral Fellowship from the Ford Foundation (JD), the National Science Foundation under grants OCE 1225801 (JD) and OCE 1233612 (AA), the Ocean and Climate Change Institute of the Woods Hole Oceanographic Institution (CH), a BIOS Grant in aid award (SM), the Sidney Stern Memorial Trust (CH and AA), as well as an anonymous donor.
    Keywords: Coral ; Superoxide ; Reactive oxygen species ; Photosynthesis ; Symbiodinium ; Stress
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  • 6
    Publication Date: 2022-10-26
    Description: © The Author(s), 2019. This article is distributed under the terms of the Creative Commons Attribution License. The definitive version was published in Roemmich, D., Alford, M. H., Claustre, H., Johnson, K., King, B., Moum, J., Oke, P., Owens, W. B., Pouliquen, S., Purkey, S., Scanderbeg, M., Suga, T., Wijffels, S., Zilberman, N., Bakker, D., Baringer, M., Belbeoch, M., Bittig, H. C., Boss, E., Calil, P., Carse, F., Carval, T., Chai, F., Conchubhair, D. O., d'Ortenzio, F., Dall'Olmo, G., Desbruyeres, D., Fennel, K., Fer, I., Ferrari, R., Forget, G., Freeland, H., Fujiki, T., Gehlen, M., Greenan, B., Hallberg, R., Hibiya, T., Hosoda, S., Jayne, S., Jochum, M., Johnson, G. C., Kang, K., Kolodziejczyk, N., Kortzinger, A., Le Traon, P., Lenn, Y., Maze, G., Mork, K. A., Morris, T., Nagai, T., Nash, J., Garabato, A. N., Olsen, A., Pattabhi, R. R., Prakash, S., Riser, S., Schmechtig, C., Schmid, C., Shroyer, E., Sterl, A., Sutton, P., Talley, L., Tanhua, T., Thierry, V., Thomalla, S., Toole, J., Troisi, A., Trull, T. W., Turton, J., Velez-Belchi, P. J., Walczowski, W., Wang, H., Wanninkhof, R., Waterhouse, A. F., Waterman, S., Watson, A., Wilson, C., Wong, A. P. S., Xu, J., & Yasuda, I. On the future of Argo: A global, full-depth, multi-disciplinary array. Frontiers in Marine Science, 6, (2019): 439, doi:10.3389/fmars.2019.00439.
    Description: The Argo Program has been implemented and sustained for almost two decades, as a global array of about 4000 profiling floats. Argo provides continuous observations of ocean temperature and salinity versus pressure, from the sea surface to 2000 dbar. The successful installation of the Argo array and its innovative data management system arose opportunistically from the combination of great scientific need and technological innovation. Through the data system, Argo provides fundamental physical observations with broad societally-valuable applications, built on the cost-efficient and robust technologies of autonomous profiling floats. Following recent advances in platform and sensor technologies, even greater opportunity exists now than 20 years ago to (i) improve Argo’s global coverage and value beyond the original design, (ii) extend Argo to span the full ocean depth, (iii) add biogeochemical sensors for improved understanding of oceanic cycles of carbon, nutrients, and ecosystems, and (iv) consider experimental sensors that might be included in the future, for example to document the spatial and temporal patterns of ocean mixing. For Core Argo and each of these enhancements, the past, present, and future progression along a path from experimental deployments to regional pilot arrays to global implementation is described. The objective is to create a fully global, top-to-bottom, dynamically complete, and multidisciplinary Argo Program that will integrate seamlessly with satellite and with other in situ elements of the Global Ocean Observing System (Legler et al., 2015). The integrated system will deliver operational reanalysis and forecasting capability, and assessment of the state and variability of the climate system with respect to physical, biogeochemical, and ecosystems parameters. It will enable basic research of unprecedented breadth and magnitude, and a wealth of ocean-education and outreach opportunities.
    Description: DR, MS, and NZ were supported by the US Argo Program through the NOAA Grant NA15OAR4320071 (CIMEC). WO, SJ, and SWi were supported by the US Argo Program through the NOAA Grant NA14OAR4320158 (CINAR). EuroArgo scientists were supported by the two grants: (1) AtlantOS funding by the European Union’s Horizon 2020 Research and Innovation Programme under the Grant Agreement No. 633211 and (2) Monitoring the Oceans and Climate Change with Argo (MOCCA) Co-funded by the European Maritime and Fisheries Fund (EMFF) Project No. SI2.709624. This manuscript represents a contribution to the following research projects for HC, CaS, and FD: remOcean (funded by the European Research Council, grant 246777), NAOS (funded by the Agence Nationale de la Recherche in the frame of the French “Equipement d’avenir” program, grant ANR J11R107-F), AtlantOS (funded by the European Union’s Horizon 2020 Research and Innovation Programme, grant 2014-633211), and the BGC-Argo project funded by the CNES. DB was funded by the EU RINGO project (730944 H2020-INFRADEV-2016-1). RF was supported by the AGS-1835576. GCJ was supported by the Global Ocean Monitoring and Observing Program, National Oceanic and Atmospheric Administration (NOAA), U.S., and the Department of Commerce and NOAA Research. LT was funded under the SOCCOM Grant No. NSF PLR-1425989. VT’s contribution was supported by the French National Research Agency (ANR) through the EQUIPEX NAOS (Novel Argo Observing System) under the reference ANR-10-EQPX-40 and by the European H2020 Research and Innovation Programme through the AtlantOS project under the reference 633211. WW was supported by the Argo Poland program through the Ministry of Sciences and Higher Education Grant No. DIR/WK/2016/12. AmW was funded by the NSF-OCE1434722. K-RK is funded by the National Institute of Meteorological Sciences’ Research and Development Program “Development of Marine Meteorology Monitoring and Next-generation Ocean Forecasting System” under the grant KMA2018-00421. CSchmid is funded by NOAA/AOML and the US Argo Program through NOAA/OOMD. MBa is funded by NOAA/AOML.
    Keywords: Argo ; Floats ; Global ; Ocean ; Warming ; Circulation ; Temperature ; Salinity
    Repository Name: Woods Hole Open Access Server
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