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  • Drosophila  (150)
  • wheat  (144)
  • Springer  (294)
  • Essen : Verl. Glückauf
  • Krefeld : Geologischer Dienst Nordhein-Westfalen
  • 2015-2019
  • 2005-2009
  • 1985-1989  (294)
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  • 101
    Electronic Resource
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    Springer
    Behavior genetics 18 (1988), S. 389-403 
    ISSN: 1573-3297
    Keywords: sexual selection ; sexual behavior ; assortative mating ; polymorphism ; Drosophila
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Psychology
    Notes: Abstract Although many experiments on laboratory stocks ofDrosophila have suggested that mate choice is a major feature of sexual selection in this organism, few attempts have been made to measure its extent in wild populations. In this study, a crossing design was used to obtain a set of 13 genetically identical independent lines representative of genotypes from an African population ofDrosophila melanogaster. They were tested for variation in sexual behavior using dyadic tests. Significant variation in orientation and vibration latencies was found for males, and in mating speed and copulation duration for both sexes. No evidence of assortative mating, either positive or negative, was found. The absence of a correlation in mating speed between males and females sharing the same genotype leads us to doubt the applicability of the notion of “male eagerness” and “female reluctancy” inDrosophila and the importance of “vigor” as a factor in mating speed. The absence of mate choice in natural populations ofDrosophila seems to us the most likely hypothesis on the basis of both theory and empirical evidence.
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  • 102
    ISSN: 1573-3297
    Keywords: reproductive behavior ; pulse song ; sine song ; acoustic spectrum ; Drosophila
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Psychology
    Notes: Abstract Digital signal processing methods have revealed spectral components inDrosophila melanogaster's andD. simulans' male courtship songs that had gone undetected in previous studies. We found that a bout of courtship hum (“sine song”) inD. simulans typically consists of a narrowband fundamental frequency, accompanied by second and third harmonics that can comprise a major fraction of the power in the signal. The pulse song spectra consisted of single broad-band peaks of highly variable frequencies, which, nevertheless, are characteristically different in these two species. Genetic elements of the newly discovered song components were examined by analysis of theD. melanogaster/D. simulans hybrid. Such males were found to be intermediate in production of sine song harmonics as well as in other parameters of courtship song, except for sine song and intrapulse frequency bandwidths, for which there may be dominant factors inD. simulans.
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  • 103
    ISSN: 1573-4927
    Keywords: wheat ; gliadins ; gel electrophoresis ; evolution ; genetic analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The gliadin composition of 78 spring durum wheat varieties has been studied by one-dimensional (Al-lactate,pH 3.1) and two-dimensional (first dimension, Al-lactate,pH 3.1; second dimension, sodium dodecyl sulfate-polyacrylamide gel) electrophoresis. Analysis of hybrids has shown that all components of the α zone of gliadin spectra are inherited together as blocks and are, probably, coded for by a cluster of tightly linked genes located on chromosome 6A. Fourteen variants of gliadin blocks have been identified, which can be classified into five families on the basis of component composition. All families but one have analogues among chromosome 6A-controlled blocks of bread wheat. The results indicate that some of the genome A diploid genotypes that were ancestors of durum wheats were also ancestors of bread wheats and that polyploid wheats were produced by repeated allopolyploidization events, as has been suggested earlier.
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  • 104
    ISSN: 1573-4927
    Keywords: wheat ; gliadins ; gel electrophoresis ; evolution ; genetic analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The gliadin composition of 78 spring durum wheat varieties has been studied by one-dimensional (Al-lactate,pH 3.1) and two-dimensional (first dimension, Al-lactate,pH 3.1; second dimension, sodium dodecyl sulfate-polyacrylamide gel) electrophoresis. Analysis of hybrids has shown that all components of the α zone of gliadin spectra are inherited together as blocks and are, probably, coded for by a cluster of tightly linked genes located on chromosome 6A. Fourteen variants of gliadin blocks have been identified, which can be classified into five families on the basis of component composition. All families but one have analogues among chromosome 6A-controlled blocks of bread wheat. The results indicate that some of the genome A diploid genotypes that were ancestors of durum wheats were also ancestors of bread wheats and that polyploid wheats were produced by repeated allopolyploidization events, as has been suggested earlier.
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  • 105
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    Plant molecular biology 10 (1988), S. 303-310 
    ISSN: 1573-5028
    Keywords: chloroplast gene ; monocot ; psbA ; transcription ; wheat ; 5′ end
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have mapped and cloned the wheat chloroplast gene (psbA) that encodes the 32 kd polypeptide of Photosystem II. The psbA gene is located in the large single copy region adjacent to one inverted repeat and is transcribed toward the latter. The sequence of the 5′ end of the wheat gene is homologous with dicot psbA genes. We have located the 5′ terminus of the wheat psbA RNA to a position 83 nt upstream of its coding region. The same psbA RNA species was capped in vitro by guanylyltransferase, establishing that its 5′ end is a transcription start site. Regions which resemble procaryotic -10 and -35 promoter elements are located immediately upstream of the wheat psbA transcription initiation site.
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  • 106
    ISSN: 1573-5028
    Keywords: wheat ; triplet protein (triticin) ; legumin ; amino acid sequence homology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have obtained several amino acid sequences from purified polypeptides of a wheat endosperm storage globulin previously described as ‘triplet’ protein. The amino acid sequence data supported by immunochemical analysis using anti-oat 12S globulin antibodies, provide definitive evidence that the triplet protein is homologous to pea legumin and related seed storage proteins of oats, rice and several dicotyledonous species. Thus, it is now proposed that the triplet protein of wheat be renamed ‘triticin’. The oat globulin antibodies also cross-reacted strongly with the high-molecular-weight (HMW) glutenin subunits which have been implicated in bread-making quality.
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  • 107
    ISSN: 1573-5028
    Keywords: mitochondrial DNA ; transfer RNA genes ; rearrangement ; recombination ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In the course of isolating tRNA genes from wheat mtDNA, we have found the same tRNAPro gene in two different Hind III restriction fragments, H-P1 (0.7 kbp) and H-P2 (1.7 kbp). Sequences immediately flanking these duplicate genes are closely related, although not identical; sequence comparisons suggest that multiple rearrangements have occurred in the vicinity of the H-P2 tRNAPro gene, relative to the H-P1 version. The chimeric nature of H-P2 is emphasized by the presence of sequences that are also found upstream of the wheat mitochondrial 26S rRNA gene, as well as sequences derived from chloroplast DNA. Comparison of H-P2 with H-P1 plus upstream sequences provides some insight into possible molecular events that might have generated H-P2. In particular, such comparisons suggest a model in which the homologous sequences in H-P2 are seen to be derived from H-P1 plus upstream sequences as a result of an intragenomic, site-specific rearrangement event, followed by amplification of the product, its fixation in the mitochondrial genome, and subsequent sequence divergence (single base changes as well as insertions/deletions of up to 50 nucleotides). The results reported here implicate particular primary sequence motifs in certain of the rearrangements that characterize H-P2.
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  • 108
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    Plant molecular biology 10 (1988), S. 251-262 
    ISSN: 1573-5028
    Keywords: mitochondria ; mitochondrial DNA ; transfer RNA ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have begun a systematic search for potential tRNA genes in wheat mtDNA, and present here the sequences of regions of the wheat mitochondrial genome that encode genes for tRNAAsp (anticodon GUC), tRNAPro (UGG), tRNATyr (GUA), and two tRNAsSer (UGA and GCU). These genes are all solitary, not immediately adjacent to other tRNA or known protein coding genes. Each of the encoded tRNAs can assume a secondary structure that conforms to the standard cloverleaf model, and that displays none of the structural aberrations peculiar to some of the corresponding mitochondrial tRNAs from other eukaryotes. The wheat mitochondrial tRNA sequences are, on average, substantially more similar to their eubacterial and chloroplast counterparts than to their homologues in fungal and animal mitochondria. However, an analysis of regions ∼ 150 nucleotides upstream and ∼ 100 nucleotides downstream of the tRNA coding regions has revealed no obvious conserved sequences that resemble the promoter and terminator motifs that regulate the expression of eubacterial and some chloroplast tRNA genes. When restriction digests of wheat mtDNA are probed with 32P-labelled wheat mitochondrial tRNAs, 〈20 hybridizing bands are detected, whether enzymes with 4 bp or 6 bp recognition sites are used. This suggests that the wheat mitochondrial genome, despite its large size, may carry a relatively small number of tRNA genes.
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  • 109
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    Theoretical and applied genetics 75 (1988), S. 468-473 
    ISSN: 1432-2242
    Keywords: Compound chromosomes ; Fitness ; Drosophila ; Assortative mating ; Pest control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The relative net fitness of a compound chromosome strain of Drosophila melanogaster was about 0.05, compared with the chromosomally normal strain from which it was derived. Based on meiotic considerations alone, the expected relative fitness was about 0.25. There were no significant differences in fertility between the compound and normal strains; the compound strain produced about 28% as many offspring as the normal strain and developed faster than the normal strain in two replicates, and slower in one replicate. The low relative fitness of the compound strain was apparently due to assortative mating, in which normal females discriminated strongly against compound males. Implications for pest control projects are dicussed.
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  • 110
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    Theoretical and applied genetics 76 (1988), S. 933-940 
    ISSN: 1432-2242
    Keywords: Gliadin ; Two-dimensional electrophoresis ; F1 monosomic analysis ; chromosomal assignment ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The gliadin components from four bread wheat cultivars: Chinese Spring, Capelle Desprez, Holdfast and Pane-247 and their monosomic F1s for the chromosomes of homoeologous groups 1 and 6 have been analyzed by two-dimensional (2-pH) polyacrylamide gel electrophoresis. Chromosomal location of gliadin genes and the allelic differences were well established by analyzing the different F1 monosomic hybrids, electrophoretical patterns and differences in relative staining intensity. A new gliadin encoded by a gene located on chromosome 6B in Chinese Spring is described. The two-dimensional patterns of gliadin in the other three varieties and the chromosomal location of their genes are reported for the first time. Relationships between gliadins in the two-dimensional patterns and the traditional system for their nomenclature are discussed.
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  • 111
    ISSN: 1617-4623
    Keywords: β tubulin genes ; Drosophila ; Mesoderm ; Neural system ; Oogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In Drosophila β tubulins are encoded by a small gene family and the four members of this family are differentially expressed. mRNAs transcribed from two of these genes, namely the β1 and β3 tubulin genes, are abundant during embryogenesis. While the β1 tubulin gene is constitutively expressed during development, β3 mRNA is restricted to two distinct phases: mid embryogenesis and metamorphosis. The transcription initiation sites are identical in both these stages and comparison of presumptive promoter regions reveals no extensive homologies between the genes. In situ localization shows β1 tubulin mRNA to be maternally expressed in the nurse cells of the egg chambers and evenly distributed during early embryogenesis. In contrast, during later stages of embryogenesis β1 tubulin transcripts are predominantly expressed in neural derivatives. The β3 tubulin gene expression is also spatially regulated, β3 mRNA being restricted to the mesoderm.
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  • 112
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    Molecular genetics and genomics 213 (1988), S. 505-512 
    ISSN: 1617-4623
    Keywords: Drosophila ; Molybdoenzymes ; cinnamon
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mutations at the cin gene display drastically lowered levels of the molybdoenzymes, xanthine dehydrogenase (XDH) and aldehyde oxidase (AO), and lack pyridoxal oxidase (PO) and sulfite oxidase (SO) activities. Certain mutations at cin also display varying degrees of female sterility, which is maternally affected. Here we characterize five new cin alleles with respect to the molybdoenzyme activities as well as the molybdenum cofactor, commonly required for molybdoenzyme activity. In complementing cin heterozygotes we find that, in addition to the previously reported unusually high levels of XDH and AO activities, there are unusually elevated levels of SO activity, as well as complementation for PO activity. The levels of immunologically crossreacting material in such heterozygotes indicate that the elevated levels of molybdoenzyme activities cannot be due to increases in the number of enzyme molecules. Measurements of the level of molybdenum cofactor activity normally present in XDH, AO, PO, and SO point to the possibility that a larger fraction of the enzyme molecules are active in these heterozygotes. The possible role of SO with respect to cinnamon's female sterility is also discussed.
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  • 113
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    Molecular genetics and genomics 212 (1988), S. 370-374 
    ISSN: 1617-4623
    Keywords: Cytotype ; Drosophila ; Gene expression ; P-element ; Vestigial
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A series of P-element insertion mutations at one site in the vestigial (vg) locus was tested for cytotype dependent effects on vg expression. The mutant phenotypes for four P-element vg alleles were suppressed when the alleles were stabilized in the P-cytotype. The suppression was observed whenever repressor-producing P-elements were present in the genome. Genetic and molecular analysis indicated that the suppression is not due to excision or other irreversible alterations of the inserts. The results are consistent with a model in which somatic P-element repressor binding to the ends of P-element inserts can modify the effects of these inserts on target gene expression.
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  • 114
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    Molecular genetics and genomics 213 (1988), S. 359-363 
    ISSN: 1617-4623
    Keywords: Autonomous replication ; Drosophila ; Plasmid retention
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Six kinds of autonomously replicating sequences (ARSs) derived from Drosophila or tobacco were inserted into the vector pDSV, constructed with pSV2-gpt and the copia long terminal repeat (LTR). The resulting ARS-containing plasmids, pDSV-ARSs, were transfected into the cultured Drosophila cells of GM1 S1cl1. Most of the plasmids remained for about 2 weeks and some for about 1 month in these cells. The retention time of the plasmid was not directly correlated with autonomously replicating activity of ARSs detected in the yeast. Two plasmids, one carrying ARS of Drosophila nuclear DNA and the other carrying tobacco DNA, showed the longest retention time in transformed cells and replication was confirmed in these cells. Some of these long lived plasmids were recovered, however, as modified forms. Other plasmids had disappeared 1 month after transfection. Two months following transfection, none of plasmids were recovered but they were detected in nuclear DNA as the integrated form. The integration patterns in all the cells transformed by different kinds of ARS-containing plasmids were similar to each other, and to the distribution pattern of copia LTR in the genome. These results suggest that copia LTR sequences contained in the pDSV-ARSs may participate in the integration process of these plasmids into Drosophila DNA.
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  • 115
    ISSN: 1617-4623
    Keywords: Drosophila ; Uncoordinated gene ; Repetitive DNA ; Type 1 insertion sequence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The technique of chromosome walking was used to isolate approximately 60 kb of DNA from the region containing the complementation group uncoordinated of Drosophila melanogaster, located in that part of the X chromosome which spans the euchromatin-heterochromatin junction. The cloned DNA can be divided into two distinct regions. The first contains sequences that are low copy number or unique and are largely conserved between strains. The second region is characterized by units repeated in tandem arrays and is polymorphic within, and between, strains. Each repetitive unit is separated by a member of an abundant sequence family, part of which is homologous to the ribosomal type 1 insertion sequence of D. melanogaster. The molecular organization of the cloned DNA was compared with that of sequences isolated from regions of intercalary heterochromatin and also with genes which have been characterized from more conventional euchromatic regions.
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  • 116
    ISSN: 1615-6102
    Keywords: Drosophila ; Myofibril assembly ; Contractile proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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  • 117
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    Molecular genetics and genomics 211 (1988), S. 381-385 
    ISSN: 1617-4623
    Keywords: Mitochondrial DNA ; Restriction-site heteroplasmy ; Transmission genetics ; Drosophila
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The composition of mitochondrial DNA (mtDNA) was analyzed in single female flies that developed from fertilized Drosophila melanogaster eggs, into which germ plasm of D. simulans had been introduced. HpaII cleavage patterns showed that all 12 individual female flies examined had developed from eggs in which 37%–71% of the total mtDNA was D. simulans mtDNA (Ds mtDNA) and the rest was D. melanogaster mtDNA (Dm mtDNA). The stability of this heteroplasmic state in these isofemale lines was monitored for seven generations at both individual and population levels. Results showed that the heteroplasmy of Dm and Ds mtDNAs was stably transmitted for at least three generations at the population level, but showed stochastic segregation at the individual level. After 4–6 generations, all individuals lost Ds mtDNA. The mechanisms of preferential loss of Ds mtDNA and of transmission of heteroplasmic mtDNA to descendants are discussed.
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  • 118
    ISSN: 1617-4623
    Keywords: Drosophila ; Minute ; Ribosomal protein ; Transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Minute loci represent a class of about 50 different Drosophila genes that appear to be functionally related. These genes may code for components of the protein synthetic apparatus. While one Minute locus has been recently shown to code for a ribosomal protein, it is not yet known whether any of the other Minute loci also code for ribosomal proteins. We have addressed this question by a combined molecular and genetic approach. In this report, a cloned DNA encoding the ribosomal protein rp21 is partially characterized. The rp21 gene maps to the same region (region 80 of chromosome 3L) as the temperature-sensitive Minute QIII gene. Using P-element mediated transformation, the rp21 gene was transformed into the germline of Drosophila. RNA blot experiments revealed that the transformed gene is expressed in transgenic flies. However, genetic complementation analysis indicated that the QIII locus and the rp21 gene are not identical. Implications of these findings for the relationship between Minutes and ribosomal protein genes are discussed.
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  • 119
    ISSN: 1573-5036
    Keywords: black gram ; boron deficiency ; borax rates ; green gram ; hollow heart ; hot-water-soluble boron ; kernel boron ; leaf boron ; peanut ; rice ; soybean ; sunflower ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effect of boron (B) on peanut and soybean was examined in two omission and one B fertilizer rate trial on a Typic Tropaqualf in Northern Thailand. The B rate trial was combined with a comparison of the response of sunflower, green gram, black gram, wheat, and rice in addition to peanut and soybean grown in irrigated rice-based cropping sequences over two years. Omitting B induced the hollow heart symptom in 10% of peanut kernels with the incidence of hollow hearts closely related to B concentration in the kernels. Omission of B had no effect on the appearance of soybean seed or on the grain yield of either soybean or peanut. In the B rate experiment, omitting B depressed grain yield by 50% in sunflower and by 40% to 80% in black gram, induced B deficiency symptoms in green gram and the hollow heart symptom in peanut kernels, but had not significant effect on the grain yield of soybean, peanuts, rice, or wheat. B deficiency apparently depressed grain yield in black and green gram by delaying or inhibiting reproductive development thus reducing pod set.
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  • 120
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    Euphytica 39 (1988), S. 137-144 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Hordeum vulgare ; barley ; boron toxicity ; variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The growth and yield of seven wheat and two barley cultivars or lines, previously found to show different degrees of boron tolerance under field conditions, were compared in a pot experiment at a range of soil boron treatments. Soil treatments ranged up to 150 mg/kg applied B. Extractable B in soils ranged up to 103 mg/kg. At the highest B treatment seedling emergence was delayed, but the percentage emergence was not reduced. The degree of boron toxicity symptom expression varied between the wheat cultivars and lines, with the two most tolerant, Halberd and (Wq*KP)*WmH)/6/12, displaying the least symptoms. The concentration of boron applied to the soil which produced a significant depression of growth and yield varied between cultivars. For example, the yield of (Wq*KP)*WmH)/6/12 was not affected at the 100 mg/kg applied boron treatment, while the grain yield for (Wl*MMC)/W1/10 was significantly reduced at the 25 mg/kg treatment. There was a linear increase in boron concentration in tillers at the boot-stage with increasing concentration of boron in the soil. The most boron tolerant genotypes had the lowest tissue boron concentrations in each of the treatments. Halberd and (Wq*KP)*WmH)/6/12 had approximately half the boron concentrations of the more sensitive genotypes at the 25 and 50 mg/kg treatments. Differential tolerance of boron within the tissue was also observed. Both Stirling and (Wl*MMC)/W1/10 had significantly reduced total dry matter and grain yields at the 25 mg/kg treatment, while the concentrations of boron in boot stage tillers at this treatment were 118 and 100 mg/kg, respectively. On the other hand, Halberd and (Wq*KP)*WmH)/6/12 had tissue boron concentrations of 144 and 131 mg/kg, respectively, at the 50 mg/kg treatment but yield was unaffected. The relative responses in the pot experiment, for wheat, were in close agreement with field results. Halberd and (Wq*KP)*WmH)/6/12 had the highest grain yields, with the lowest concentrations of boron in the grain when grown under high boron conditions in the field. In pots these two genotypes proved to be the most tolerant of boron. For barley the advantage in grain yield in the field, expressed by WI-2584 compared with Stirling, was not repeated in pots. WI-2584 was, however, more tolerant than Stirling on the basis of total dry matter production. The results show that useful variation in boron tolerance exists among wheat, and that breeding should be able to provide cultivars tolerant to high levels of boron.
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  • 121
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    Plant and soil 110 (1988), S. 140-142 
    ISSN: 1573-5036
    Keywords: Gaeumannomyces graminis ; roots ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A root assessment tray was designed for the meticulous assessment of take-all on wheat seedling roots from soil bioassays. Subsequently, the detection of lateral root infections (in addition to the more obvious infections on main axes of seminal roots) resulted in increased estimates of propagule numbers of the take-all fungus (Gaeumannomyces graminis var.tritici) for 196 of the 368 soil samples bioassayed in a field study conducted in Western Australia between 1984 and 1986.
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  • 122
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    Plant and soil 105 (1988), S. 169-178 
    ISSN: 1573-5036
    Keywords: development ; fibrous root growth ; geotropism ; root deflection ; root model ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A model is described which simulates the growth of fibrous root systems. The root growth is specified in terms of growing time, numbers of axes, initiation times of axes, growth rates and branching characteristics of the roots, and characteristics governing the direction of root growth. The model generates a representation of the root system in which the locations of all branches and root tips are recorded in three-dimensional coordinates, and updates this representation in discrete time steps until the specified growing time is reached. Data are presented from a simulation of wheat root growth by the model. The simulated root system is represented pictorially and also graphically in the form of root length and root tip number profiles which are stratified by branching order class. The pictorial representations produced by the model are much more realistic than any which have been produced by past root growth models, and the graphical representations show trends in root length and root tip numbers which are the same as those commonly observed in real roots.
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  • 123
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    Plant and soil 109 (1988), S. 128-130 
    ISSN: 1573-5036
    Keywords: nitrogen ; root pruning ; shoot ; root ratio ; Thornley's model ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In two experiments, wheat plants growing in solutions of different nitrogen concentration were subjected to root pruning. In higher concentrations of nitrogen the growth rate was higher, and the proportional allocation of growth to shoot higher, but pruning did not affect the allocation of growth at either level of nitrogen. This result gives no support to Thornley's source-sink model of the control of shoot: root ratio.
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  • 124
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    Plant and soil 109 (1988), S. 195-198 
    ISSN: 1573-5036
    Keywords: Anguina tritici ; Corynebacterium tritici ; disease complex ; inoculation methods ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Wheat,Triticum aestivum L. cv. Mexipak was artificially inoculated withAnguina tritici (Steinb.) under field conditions. Nematode inocula: 0, 0.1, 0.2 and 0.3 g of seed galls/50 seeds or, germinated seeds of wheat (sown in a 1-m long row) were either added as intact seed galls or as a suspension of 2nd stage juveniles. Inoculation of ungerminated seeds with juveniles produced the highest incidence of ear-cockle disease. Whereas, intact seed galls inocula produced the highest incidence of tundu disease. Ear-cockle incidence was always greater than that of tundu at all treatments. The highest reduction in grain yield was associated with the treatments that caused the highest incidence of tundu disease.
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  • 125
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    Plant and soil 112 (1988), S. 261-266 
    ISSN: 1573-5036
    Keywords: crown rot ; Fusarium graminearum ; interactions ; wheat ; zinc deficiency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Wheat plants were grown at three levels of zinc nutrition in potted soil under controlled conditions. The surface soil in half of the pots was inoculated with a thin layer of milled chaff colonized byFusarium graminearum Group 1. Forty days after sowing, the plants were assessed for dry matter production and the extent of colonization by the pathogen. The concentration of zinc in the plant tissues was also determined. The zinc status of the plants ranged from severe deficiency through subclinical deficiency to sufficiency. The extent of colonization above the point of infection was decreased significantly by increasing the level of zinc supply. However, colonization of the seminal or secondary roots was not affected by zinc supply, nor was the incidence of infected plants. The unidirectional effect on resistance suggests that zinc has modified the contribution of the xylem flux to the upward spread of the pathogen.
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  • 126
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    Plant and soil 111 (1988), S. 171-175 
    ISSN: 1573-5036
    Keywords: hormones ; potassium status ; potassium transport ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
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  • 127
    ISSN: 1573-5036
    Keywords: barley ; boron toxicity ; genotypic variation ; mineral nutrition ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The mechanism of resistance toB toxicity in barley and wheat was studied in a solution culture experiment using several cultivars displaying a large range of sensitivity to excessB supply. Plants were cultured for 35 d atB concentrations ranging from normal to excessive (15 to 5000 μM, respectively) then examined for dry matter production and theB distribution between roots and shoots. In both species, increasedB supply was accompanied by increased tissueB concentrations, development ofB toxicity symptoms and depressed growth. At each level ofB supply, however, resistant cultivars accumulated considerably lessB than did sensitive cultivars, in both roots and shoots. Even at the lowestB supply, at which noB toxicity symptoms developed and growth was not affected, resistant cultivars maintained relatively low tissueB concentrations. No cultivar displayed an ability to tolerate high tissueB concentrations. These results indicate that sensitivity toB toxicity in barley and wheat is governed by the ability of cultivars to excludeB. If theB concentrations of tissues is used to indicate resistance toB toxicity, then cultivars have the same ranking whether cultured at a normal or excessB supply.
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  • 128
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    Plant and soil 110 (1988), S. 101-109 
    ISSN: 1573-5036
    Keywords: bean ; influx rate ; onion ; phosphorus efficiency ; rape ; ratio ; root-shoot ; ryegrass ; spinach ; tomato ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Plant species differ in their P efficiency,i.e. the P content in soil needed to reach their maximum yield. The differences in external P requirements can be atributed to either a lower internal P requirement for optimum growth or higher uptake efficiency of the plant. The objective of this research was to investigate the reasons for different P efficiencies of seven plant species. Onion, ryerass, wheat, rape, spinach, tomato and bean were grown in a P-deficient subsoil fertilized with 0, 2, 5, 10, 20, 40 and 80 mg P 100 g−1. All species showed a strong yield increase due to P fertilization. To reach 80% of maximum yield onion and tomato needed 17 and 11 mg P 100 g−1 respectively, corresponding to a soil solution concentrations of 6.9 and 5.7 μmol P l−1, whereas ryegrass, wheat and rape needed about 5 mg P 100g−1 corresponding to only 1.4 μmol P l−1 in soil solution. These differences in external P requirement cannot be explained by differences in their internal P requirement since onion, with the highest external P requirement, only contained 0.14% P in the shoot at 80% of maximum yield, while wheat, as the most P efficient species, contained 0.28%. P efficiency was related to the uptake efficiency of the plant which is determined by both root-shoot ratio and absorption rate per unit of root (influx). Species of low efficiency such as onion, tomato and bean had low influx rates and low root-shoot ratios, whereas species of medium to high efficiency had either high influx rates (rape and spinach) or high root-shoot ratios (ryegrass and wheat). The combination of both high influx rate and high root-shoot ratio was not found in any of the species studied.
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  • 129
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    Plant and soil 111 (1988), S. 25-35 
    ISSN: 1573-5036
    Keywords: carbohydrate accumulation ; chloride ; salinity-phosphorus interaction ; sodium ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Foliar inorganic ion and carbohydrate concentrations were determined in wheat plants treated with factorial combinations of phosphorus fertilizer and NaCl in a glasshouse experiment. Growth reductions and visual symptoms of salt toxicity were minimized when phosphorus nutrition was adequate, and were intensified by phosphorus deficiency. Foliar sodium and chloride accumulated up to 4.0–5.5% d.w. with salinity treatment. However, ionic concentrations within corresponding leaves or distributions between leaves of plants with different phosphorus treatments were not influenced by phosphorus treatment and had no relationship to the severity of salt toxicity symptoms. This suggests that phosphorus deficiency reduced the cellular tolerance for ion accumulation. A combination of phosphorus deficiency and salinity induced an accumulation of foliar starch and sucrose despite substantial reductions in net CO2 assimilation rates. This accumulation did not occur if phosphorus nutrition was adequate, which is consistent with the roles of phosphorus in carbohydrate metabolism. It is proposed that adequate phosphorus nutrition is essential for effective ion compartmentation by contributing to efficient carbohydrate utilization in salt-stressed wheat.
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  • 130
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    Plant and soil 107 (1988), S. 19-23 
    ISSN: 1573-5036
    Keywords: Gaeumannomyces graminis ; pectolytic enzymes ; Phialophora graminicola ; Phialophora sp. ; virulence ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Gaeumannmyces graminis var.tritici (Ggt), Phialophora sp. (lobed hyphopodia) andPhialophora graminicola vere grown in a liquid medium with pectin and on autoclaved wheat roots (root media) and the activity of pectolytic enzymes in culture filtrates was measured. Most strains of the fungi exhibited polygalacturonate trans-eliminase activity but no pectin methylesterase activity was detected.Ggt polygalacturonase was found in culture filtrates from all the media used whilePhialophora sp. did not exhibit activity of this enzyme in the unbuffered root media. No polygalacturonase activity was demonstrated forP. graminicola. A correlation was found (r=0.548) betweenin vitro polygalacturonase activity and the pathogenicity ofGgt to wheat seedlings.
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  • 131
    ISSN: 1573-5036
    Keywords: mineral composition ; short-term flooding ; sodic soil ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In sodic soils of the Indo-Gangetic alluvial plains of Northern India, flooding for short periods often occurs during the growing season of wheat, leading to low yields. A field study was therefore conducted to evaluate the effects of short-term flooding on growth, yield and mineral composition of wheat (Triticum aestivum Linn. emend. Fiori and Paol) in a sodic soil (pH 8.9, exchangeable sodium percentage 25). Flooding wheat for 2,4 and 6 days at the time of first irrigation (25-day old plants), significantly reduced tillering, plant height, delayed head emergence and resulted in 17.6, 29.0 and 46.7% reduction in grain yield, respectively, Flooding decreased oxygen diffusion rate (ODR) values, restricted root grwoth and reduced ion uptake, especially of N, P, K, Ca, Mg and Zn and led to higher absorption of Na, Fe and Mn. Under the conditions of this experiment, the reduced growth and yield of wheat resulting from short-term flooding was not due to Mn, Fe and Na toxicity but may be due to reduced uptake of nutrients resulting from O2 deficiency in the soil.
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  • 132
    ISSN: 1573-5044
    Keywords: wheat ; somatic embryogenesis ; embryogenic callus ; 3,6-dichloro-o-anisic acid ; 2,4-dichlorophenoxyacetic acid ; 6-furfurylaminopurine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Nine experiments were conducted to determine effects of various culture medium addenda on induction of embryogenic calli from immature embryos of a responsiveTriticum aestivum L. genotype (PCYT 10). Effects were quatified by counting somatic embryos (embryoids) per callus. Optimal auxin concentrations to induce and maintain somatic embryogenesis were 3.62 μM 2,4-dichlorophenoxyacetic acid (2,4-D) or 9.05 μM 3,6-dichloro-o-anisic acid (dicamba). In general, dicamba permitted formation of significantly more embryoids than 2,4-D. Kinetin (6-furfurylaminopurine) at 2.56 μM or 4.65 μM significantly increased percentage scutellar callus when added to 2,4-D or dicamba-containing medium, respectively. Kinetin at 4.65 μM signficantly increased the numbers of embryoids formed when added to medium containing either synthetic auxin. Significantly fewer embryoids formed when cultures were incubated under diffuse light (16-h photoperiod). Casein hydrolysate (200 mgl-1) or L-arginine (0.23 mM) had no effect on numbers of embryoids formed, whereas L-tryptophan (0.20 mM) enhanced such formation with 2,4-D and decreased such formation with dicamba. Two additional experiments generally demonstrated that response to auxin source in the genotypes ND 7532, PCYT 20, Yaqui 50, and Oasis was similar to that in PCYT 10. The higher molar concentration of dicamba required to induce embryogenic callus coupled with more evident embryoid precocious germination and a more rapid rate of tissue necrosis upon extended incubation without subculture suggests that dicamba is metabolized more rapidly than 2,4-D inT. aestivum callus cultures.
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  • 133
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    Plant cell, tissue and organ culture 12 (1988), S. 299-304 
    ISSN: 1573-5044
    Keywords: callus ; plant regeneration ; isozymes ; wheat ; barley
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Callus cultures fron non-organogenic, young and one-year old, and morphogenic calli were used to assess the value of isozymes analysis for the prediction of morphogenic capacity by studying esterase, peroxidase and acid phosphatase. Basic isozyme patterns of each enzyme for the callus were retained in all the callus stages and in the callus which has differentiated into shoots. With the development of shoot and/or root some conspicuous isozymes appeared for esterase and acid phosphatase and some disappeared for peroxidase. As the isozyme changes became apparent only after shoot or root initiation these enzymes could not be used as markers to distinguish between morphogenic and non-morphogenic calli.
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  • 134
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    Nutrient cycling in agroecosystems 15 (1988), S. 123-136 
    ISSN: 1573-0867
    Keywords: corn ; soybean ; wheat ; maximum yield ; optimum yield ; Bray P1 ; exchangeable K
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Data from 32 years of a rotation-fertility experiment were analyzed to determine the average P and K application rates required for maximum yield and for optimum yield. A four-year rotation of corn, soybean, wheat, hay was used for the first 10 years and then changed to corn-1, soybean, wheat, corn-2. Rates of P application per 4-year rotation ranged from 0 to 196 kg ha−1 and for K from 0 to 558 kg ha−1. Multiple regression equations were fitted to the mean yields per 4-year rotation for the response of each crop to P and K applications. The range in P application rates in kg of P per 4-year rotation required to get maximum yields of corn was 118 to 172, for soybeans was 134 to 150, and for wheat was 116 to 138. The range in K application rates in kg K per 4-year rotation to get maximum yields of corn was 378 to 411, for soybeans was 324 and 476, and for wheat was 11 to 323. For rates of application where P and K added exceeded crop removals, soil test P and K increased linearly with the cumulative positive balance of P and K. Where crop removal exceeded application rate, no relation was found between crop removal and soil test.
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    Plant growth regulation 7 (1988), S. 3-17 
    ISSN: 1573-5087
    Keywords: Abscisic acid (ABA) content ; barley ; genetic variation ; grain weight ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Two genetically related wheat lines growing in cabinets were given different temperatures during grain filling, and abscisic acid (ABA) was measured in whole grains by gas chromatography with an electron-capture detector. Three genetically related barley lines grown in the field were assayed for ABA content in endosperm and embryo fractions separately by radiommunoassay. Maximum grain growth rate and final weight per grain of the two wheat lines differed by 50–60% at low temperature and 30–40% at high temperature. During grain development two peaks in ABA level were observed at low temperature but only one at high temperature. At times when differences in grain growth rate between genotypes and between temperature treatments were large, the corresponding differences in ABA concentration were small. In barley, one line (Iabo 14) had 30% heavier grains than the other two (Onice and Opale). Endosperm ABA concentrations showed no clear differences between genotypes until grain filling was nearly complete. Embryo ABA levels were up to 10-times greater than those in the endosperm, with Opale having significantly less ABA in the embryo than the other two cultivars. Our experiments did not provide evidence for a causal relationship between ABA levels during grain filling and grain growth rate or final weight.
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  • 136
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; breeding ; preharvest sprouting ; heritability ; variation ; α-amylase ; falling number
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Preharvest sprouting resistance is a major breeding criterion in many regions where white wheat (Triticum aestivum L.) is produced. Availability of genetic sources of sprouting resistance should significantly advance this goal. Objectives of the present investigation were to determine heritabilities and genetic variation of sprouting resistance in progeny of six susceptible genotypes and Clark's Cream, a cultivar with excellent sprouting resistance. Sixty-five randomly selected F2:F3 lines from the six parental combinations were evaluated in blocks-within-replication designs at two locations during 1984–85. Falling number, α-amylase activity, and sprouting percentage in simulated rain were usually more favorable in F2:F4 grain than in grain of the susceptible parents and frequently equalled levels in grain of the resistant parent. Broad sense heritability estimates were moderate to high for falling number and α-amylase activity, low to moderate for visual sprouting, and inconsistent for embryo germination in ABA. Phenotypic correlations with sprouting were significant most often for falling number and least often for the agronomic traits, days to heading and kernel weight. We concluded that increased preharvest sprouting resistance is an attainable objective when genetic sources of strong resistance and appropriate selection criteria, such as sprouting after simulated rain, are used.
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  • 137
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; dormancy ; gibberellic acid ; pre-harvest sprouting
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Gibberellic acid, cold (4°C) and a combination of these two treatments were tested for use in breaking dormancy in 27 lines of white-grained wheat with varying levels of resistance to pre-harvest sprouting. Germination increased in all lines treated with gibberellic acid. Dormancy could be broken with 1 μM gibberellic acid. Response to cold varied. A combination of gibberellic acid and cold treatment was the most effective. This technique has been found useful in treating seed in a breeding program aimed at producing wheats with resistance to pre-harvest sprouting.
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    Euphytica 39 (1988), S. 7-25 
    ISSN: 1573-5060
    Keywords: Hordeum vulgare ; barley ; Triticum aestivum ; wheat ; cytogenetics ; chromosome identification ; gene localisation ; interspecific gene transfer ; chromosome elimination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Achievements and limitations regarding three aspects of cytogenetic research in barley and common wheat are illustrated and discussed. Unambiguous chromosome identification has become possible through the application of chromosome banding techniques, mainly C-banding, N-banding and Ag-banding. Gene localisation studies have yielded a vast amount of information regarding the genetic architecture of barley and wheat. Many genes have been allocated to specific chromosomes, and linkage studies have been carried out with some of these genes. There is growing evidence for a considerable discrepancy between distances on the genetic linkage maps and the physical maps of barley and wheat chromosomes. Although barley can be hybridised with most species of the genus Hordeum and with several species of related genera, interspecific gene transfer is very rare and barley breeding can presently make use of the gene pool of only one wild species, viz. H. vulgare ssp. spontaneum. For wheat breeding, the gene pools of species of the genus Triticum and species of related genera are accessible. Several methods have been developed to achieve gene transfer. Genome interactions in interspecific hybrids result in spatial separation of the parental genomes, in nucleolar competition, and sometimes in chromosome elimination and the formation of haploids.
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  • 139
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; preharvest sprouting ; dormancy ; α-amylase ; falling number ; inhibitors ; embryo response
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Resistance to preharvest sprouting has been observed in several white wheat (Triticum aestivum L.) genotypes, but the mode of resistance has not been determined. Studies were conducted to characterize the strong preharvest sprouting resistance in Clark's Cream genotype to facilitate breeding for the trait. Clark's Cream and five other hard red and white wheat genotypes were grown in the glasshouse and field and effects of simulated rain, embryo maturity, inhibitors in floral parts and GA, endogenous inhibitors, and ABA and GA combinations on sprouting were measured. The six genotypes differed significantly in sprouting after simulated rain, α-amylase activity, and response to GA and ABA. Embryo maturity and inhibition levels in floral parts and caryopses were nearly similar for all genotypes. Clark's Cream appeared to differ most in high embryo sensitivity, which was fairly nonspecific for ABA, GA, and endogenous inhibitor, and in low α-amylase production during sprouting. Breeding procedures that lead to the preharvest sprouting resistance of Clark's Cream are discussed.
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  • 140
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    Euphytica 38 (1988), S. 105-111 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; tillering ; heritability ; gene effects ; epistasis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Effective utilization of uniculm wheat (Triticum aestivum L.) germplasm in breeding programs requires an understanding of the inheritance of the uniculm character. The parents, F1, F2, and first generation backcrosses (B1 and B2) of two crosses, each utilizing a uniculm spring wheat line and a locally adapted winter wheat cultivar, were space planted in an experiment to acquire information regarding the genetic control of culm number. Significant F1 deviations from midparent values revealed the presence of substantial non-additive gene effects. The scaling tests of Mather and the joint scaling test detected the presence of epistasis. Hayman's six-parameter model revealed that a negative dominance effect provided the major contribution to variation in culm number, while additive x additive and dominance x dominance gene effects were of considerable importance.
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  • 141
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; grain yield ; selection ; simple mass selection ; gridded mass selection ; rhombic grid selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary An experiment was conducted in Winnipeg, Manitoba, Canada to: (1) ascertain the efficiency of gridded mass selection; (2) to determine if additional selection efficiency can be achieved through refinement of the gridded mass selection procedure; and (3) to determine if the degree of homozygosity has an effect on response to single-plant selection. Three selection methods [simple mass selection (SMS); gridded mass selection (GMS); and a new rhombic grid selection (RGS)] were applied to four populations [a conventional F2 (CON-F2); an intermated F2 (INT-F2); and 2 partial backcrossed populations] developed from a biparental cross involving the Canadian spring wheat cultivars, ‘Glenlea’ and ‘Sinton’. Averaged over the 4 populations, a single cycle of RGS (10% selection intensity) increased yield by about 9.5%, whereas GMS and SMS increased yield by approximately 4.5% and 3.1%, respectively. The degree of homozygosity of individual plants that make up the population appeared to have an effect on the efficiency of selection. In general, selection was more efficient in the two partial backcrossed populations, and least efficient in the CON-F2 and INT-F2 populations. The largest response to selection (15.9%) was attained when RGS was applied in the backcross to Glenlea population, and the lowest response (1.2%) when SMS was enforced in the CON-F2 population. This paper represents the first report on the efficiency of rhombic grid selection for yield improvement in cereal crops.
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  • 142
    ISSN: 1573-5036
    Keywords: Gaeumannomyces graminis var.tritici ; rye grass ; Trichoderma hamatum ; T. harzianum ; T. koningii ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Trichoderma hamatum, T. harzianum andT. koningii were isolated from wheat and rye-grass roots from a field in Western Australia. Frequency of occurrence ofTrichoderma spp. was higher on roots subjected to washing only, for both wheat and rye-grass than the roots which were surface-sterilized with 0.6% or 1.25% NaOCl.Trichoderma spp. were recovered at a higher frequency on PDA amended with lactic acid (pH 4.5) than on PDA alone (pH 5.6) or PDA with streptomycin. In general,Trichoderma spp. were isolated at a higher frequency from roots of wheat than that of rye-grass.T. hamatum occurred at a higher frequency in rye-grass roots than in wheat, whereasT. harzianum was more common in roots of wheat than in rye-grass, especially in seedling and milky ripe stages.T. koningii was recovered at a higher frequency from roots at seedling stage of rye-grass than wheat, the reverse being true at tillering stage.T. koningii was not recovered from roots of either host in any sampling when they were surface sterilized with 1.25% NaOCl. The take-all fungus was isolated from wheat and rye-grass roots more frequently at tillering and stem extension stages than others. It was severely pathogenic to both hosts in sterilized and non-sterilized soil. Addition of lactic acid, HCl or streptomycin to PDA did not affect the growth of theTrichoderma spp. tested, but the growth was slower on Martin's medium than on other media. In generalT. harzianum andT. koningii grow faster thanT. hamatum. The growth of the three species were not different at 20 and 25°C, but at 15°c growing of all species was significantly reduced. Incorporation of lactic acid into PDA prevented the bacterial growth in all treatments. Streptomycin too reduced but to a lesser degree than lactic acid. Surface sterilization with NaOCl decreased the recovery of both bacteria and fungi. T. hamatum andT. koningii reduced the mortality of wheat and rye-grass plants inoculated with the take-all fungus in sterilized and non-sterilized soil, whereT. harzianum did not protect wheat or rye-grass from infection by the take-all fungus.
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  • 143
    ISSN: 1573-5036
    Keywords: peas ; plant genetics ; plant growth ; Pseudomonas ; rhizosphere bacteria ; Serratia ; symptoms ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Spring wheat (Triticum aestivum L.) and pea (Pisum spp.) genotypes were tested for reaction to root inoculation with rhizosphere bacteria affecting plant growth. Plant response was studied in greenhouse experiments after treatment of seedlings with bacteria suspended in nutrient broth. Significant genotype variation was found in both wheat and pea in terms of shoot dry weight and severity of bacteria-induced leaf symptoms. For most bacterial isolates tested, there was good correlation between ratings of leaf symptoms 7 to 14 days after inoculation and growth inhibition measured after four weeks. Interactions between isolates and plant genotypes were significant in both wheat and pea (P=0.0024 and 0.0001, respectively), but genotypes with sensitivity or tolerance to most isolates could be distinguished. In an outdoor pot experiment, two of the bacterial isolates caused delayed plant development and differential decreases in grain yield of wheat genotypes. The hypothesis that the reaction of wheat genotypes to the tested becteria was related to their influence on bacterial establishment in the rhizosphere could not be substantiated.
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  • 144
    ISSN: 1573-8477
    Keywords: Drosophila ; longevity ; mate tolerance ; body size ; flight ; life history
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Three likely traits were examined for their possible connection with increased life span in strains ofDrosophila melanogaster selected for longevity. First, pairing with males caused a substantial reduction in survival of females from the short-lived control strain but, long-lived females were relatively unaffected. A significant component of the improvement in selected females is, therefore, increased tolerance to the presence of mates. Females only slightly affected male survival in either long- or short-lived populations. Selected strains survive substantially better than controls independently of any effect of mate presence. The (dry) weight of whole flies is equivalent in long- and short-lived populations. Variation in body size does not appear to contribute significantly to extended longevity here. A third character, the duration of tethered flight, was found to be from three to five times greater in long-lived populations than controls. This suggests the existence of a common physiological basis of longevity to which multiple components contribute in adaptive improvement.
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    Entomologia experimentalis et applicata 43 (1987), S. 193-201 
    ISSN: 1570-7458
    Keywords: Leptopilina boulardi ; Cynipidae ; Hymenoptera ; parasitoid ; Drosophila ; Diptera ; field egg laying strategy ; functional response ; switching
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Le concept de réponse optimale d'un parasite vis-à-vis de l'hôte le plus favorable pour son développement demeure surtout théorique et n'a pu être vérifié que dans les conditions de laboratoire. Nous avons montré que Drosophila melanogaster s'avère être, par rapport à D. simulans, l'hôte le plus favorable pour le développement du cynipide parasite Leptopilina boulardi. Une étude sur le terrain a démontré que ce parasite présente une réponse fonctionnelle densité dépendante vis-à-vis de D. melanogaster et non vis-à-vis de D. simulans, avec un effet de bascule. D'autre part, il s'avère que ce parasite exploite beaucoup mieux son hôte, en évitant le superparasitisme, ceci étant démontré au laboratoire et dans la nature. Enfin, il apparaît qu'il est capable d'allonger sa période de ponte lorsque cet hôte est rare, ce qui ne se produit pas avec D. simulans.
    Notes: Abstract The hypothesis of optimal host species selection predicts that when a parasitoid has the choice between two host species, it will choose the species thay gives the best survival chances for its progeny. We confirmed this hypothesis by laboratory experiments with Leptopilina boulardi Barb. et al., a cynipid parasitoid which prefers Drosophila melanogaster Meigen (the host species most suitable for parasitoid survival) above D. simulans Sturt. As far as fitness parameters are concerned, the fertility of L. boulardi is higher with D. melanogaster; the egg laying can be spread out over a long period when this host is relatively scarce. This does not occur with D. simulans in which parasitic oviposition stops soon when this host is not abundant. Investigations of this foraging strategy were done under more complex natural conditions. We found that L. boulardi has a type III functional response with D. melanogaster only; furthermore, it seems that a switching effect may exist with this host. Parasitoid females appear to distribute their eggs more regularly on D. melanogaster, thus avoiding superparasitism. This seems to be independent of the relative frequency of this host. However, superparasitism of D. simulans did occur more frequently when this host was scarce.
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    Nutrient cycling in agroecosystems 12 (1987), S. 201-218 
    ISSN: 1573-0867
    Keywords: relative effectiveness ; apatites ; wheat ; initial and residual effectiveness ; bicarbonate extractable phosphorus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The initial and residual agronomic effectiveness of six apatite rock phosphates from India, one from the USA (North Carolina) and one from Australia (Queensland) were evaluated in a pot trial with wheat on a lateritic soil. All of the Indian rock phosphates were very poor sources of phosphorus. Values of initial effectiveness relative to monocalcium phosphate ranged from 〈 0.0001 to 0.02 and from 〈 0.0001 to 0.008 for measurements based on yield and phosphorus uptake respectively. The residual effectiveness relative to freshly applied monocalcium phosphate was determined by growing a second crop on the fertilized soils. The effectiveness of the Indian rock phosphates remained very low ranging from 〈 0.0001 to 0.002 and from 〈 0.0001 to 0.0004 for yield and phosphorus uptake respectively. Queensland and North Carolina rock phosphates were much superior to the Indian sources with initial effectiveness values in terms of yield of 0.08 and 0.37 and residual effectiveness values of 0.02 and 0.15 respectively. For each crop there was a single relationship between yield and phosphorus uptake (i.e. internal efficiency) for all phosphorus sources showing that variations in yield response were due solely to differences in phosphorus availability. Sodium bicarbonate extractable phosphorus values for fertilized soils sampled shortly after fertilization were not predictive of yield unless different calibration curves were used for the different phosphorus fertilizers.
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    Nutrient cycling in agroecosystems 13 (1987), S. 191-197 
    ISSN: 1573-0867
    Keywords: chemical extractants ; critical levels ; semi-arid region soils ; wheat ; zinc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract To find critical tissue levels of Zn for wheat, and to evaluate various chemical extractants, a screen-house experiment was conducted on 21 diverse soils representing semi-arid regions in Haryana State, India. The extractants differed in the amounts of Zn extracted and the order was: 0.1 N HC1 〉 EDTA-NH4OAc 〉 EDTA-(NH4)2CO3 〉 DTPA + CaCl2. The amounts (mg kg−1) of extractable Zn associated with a yield reduction of 20% were: DTPA + CaCl2, 0.60; EDTA — (NH4)2CO3, 0.80; EDTA-NH4OAc, 0.92 and 0.1 N HCl, 1.20. The corresponding critical Zn concentration in ten weeks old plants was found to be 17 mg gm−1. The DTPA + CaCl2 method gave the best correlation (r = 0.85) between extracted Zn and Bray's per cent yield. It is recommended for assessing Zn status of soils of semi-arid region.
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  • 148
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    Development genes and evolution 196 (1987), S. 372-375 
    ISSN: 1432-041X
    Keywords: Pattern formation ; Morphogenesis ; Drosophila ; Growth ; Imaginal disc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A comparison of the morphogenetic maps of the notum anlage of Drosophila melanogaster derived from the gynandromorph data and mosaics induced by somatic crossing-over during the first instar larval stage revealed that practically no major morphogenetic movements occur in the development of the anlage between the blastoderm and first instar larval stages and the adult stage. By comparing the morphogenetic map derived from gynandromorphs and the fate map derived from data on the transplantation of fragments of the mature wing imaginal disc, it was observed that no major morphogenetic movements occur in the notum anlage between the stages of the allocation of the disc and the mature disc. The results are consistent with the observations of other authors concerning the larval development of eye-antenna, wing and leg discs.
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  • 149
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    Development genes and evolution 196 (1987), S. 434-444 
    ISSN: 1432-041X
    Keywords: Morphogenesis ; Cell Surface ; 20-Hydroxyecdysone ; Drosophila
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    Topics: Biology
    Notes: Summary Polyclonal antibodies (anti-P116 and anti-P93) specific for two different hormone-dependent cell surface glycoproteins (P116 and P93) from Drosophila S3 cells have been produced. Anti-P116 and anti-P93 each immunoprecipitate substantially more of P116 and P93, respectively, from extracts of iodinated hormone-treated S3 cells compared to controls. Both antigens are present in control and 20-hydroxyecdysone treated imaginal discs, although apparent increases in antigen content are associated with hormone treatment. Immunofluorescent staining of whole discs with anti-P116 and anti-P93 reveals increased amounts of both antigens at the surface of hormone-treated discs compared to controls. Both antibodies were used to characterize the expression of their respective antigens during embryonic development, and both antibodies were found to recognize in embryos a third developmentally-regulated antigen with a relative mobility of approximately 220000. Our results indicate, at least in the case of P116 and P93, that 20-hydroxyecdysone-dependent cell surface antigens in imaginal discs may be regulated both by increasing the amounts of constitutively present proteins, and possibly through biochemical modifications, altering the localization of these proteins from a cytoplasmic to a cell surface domain.
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  • 150
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    Development genes and evolution 196 (1987), S. 69-77 
    ISSN: 1432-041X
    Keywords: Neurogenesis ; Drosophila ; Neuronal differentiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The stereotyped segmental and dorso-ventral organization of the peripheral nervous system (PNS) of Drosophila embryos allows the identification of all the neurons in the body wall. Distinct classes of neurons are distinguishable according to their location, the targets they innervate, the particular shape of their dendrites and their cell size. Those neurons innervating external sensory structures (es) and chordotonal organs (ch) have single dendrites and have been previously described (Ghysen et al. 1986; Dambly-Chaudiere and Ghysen 1986; Campos-Ortega and Hartenstein 1985). We describe here the identity and morphological features of three other classes of neurons in the body segments which have multiple dendrites (md neurons): 1) neurons that give rise to elaborate dendritic arborisations (da neurons); 2) neurons that have bipolar dendrites (bd neurons); 3) neurons that arborize around particular tracheal branches (td neurons). The thoracic hemisegment (T2 and T3) contains 13 da, one bd, one td, 21 es and four ch neurons; the abdominal hemisegment (A1 to A7) contains 14 da, three bd, three td, 15 es and eight ch neurons. The arrangement of the segmented peripheral neurons is highly invariant and provides a favorable assay system for the genetic analysis of neurodevelopment.
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  • 151
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    Keywords: Imaginal disc ; Evagination ; Surface peptides ; Radiolabeling ; Hormone depending ; Drosophila
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    Topics: Biology
    Notes: Summary Unevaginated and evaginated Drosophila imaginal discs were surface-labeled with 125I. Relative labeling was greater in eleven peptides and lower in three peptides of evaginated discs compared to unevaginated discs. These results are compared to the effects of 20-hydroxyecdysone (20-HOE) on metabolic labeling of membrane proteins fractionated from imaginal discs, and on cell surface labeling of a hormone-responsive Drosophila tissue culture line. A group of 35S-methionine labeled membrane fraction peptides whose metabolic labeling is 20-HOE dependent have isoelectric points and apparent molecular weights very similar to those of a group of proteins only labeled in iodinated evaginated discs, supporting the conclusion that these are hormone-dependent, cell surface proteins (Rickoll and Fristrom 1983). Based upon two-dimensional gel electrophoretic and immunological criteria three of the proteins showing increased labeling in evaginated discs are related to three proteins induced by 20-HOE in tissue culture cells. Two different subsets of radiolabeled peptides were observed in the imaginal discs based upon detergent solubility. Some of the proteins which are soluble in NP-40 plus urea but insoluble in NP-40 alone may be localized in the basal lamina of the imaginal discs, a structure which labels heavily with 125I and is lacking in tissue culture cells. In discs, the majority of hormone-dependent changes in radiolabeled peptides were seen in the fraction solubilized by NP-40 and urea with a sulfhydryl reducing agent, while in tissue culture cells, the majority of differences is seen in the fraction solubilized by NP-40 only. We speculate that these proteins may be involved in similar processes, e.g., cell rearrangement, that occur during both disc morphogenesis and 20-HOE induced aggregation in tissue culture cells.
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  • 152
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    Cellular and molecular life sciences 43 (1987), S. 445-446 
    ISSN: 1420-9071
    Keywords: Cell survival ; photoreactivation ; insect cell ; Drosophila ; colony formation ; DNA repair ; ultraviolet radiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Cell survival and photoreactivation of 254 nm ultraviolet (UV) light damage in a wild typeDrosophila cell line was assayed by colony formation in liquid medium. Fo, Fq, and extrapolation number for the exponential portion of survival curves are 21 J/m2, 3.6 J/m2, and 1.5 for non-photoreactivated cells and 110 J/m2, 11.2 J/m2, and 1.3 for those exposed to photoreactivating light. Maximal photoreactivation occurs at the 100 J/m2 region of the curve. At 10 and 50% survival, 75–80% of the UV damage was photoreactivable.
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  • 153
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    Development genes and evolution 196 (1987), S. 191-201 
    ISSN: 1432-041X
    Keywords: Wing imaginal disc ; Tissue culture ; Metamorphosis ; Drosophila
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    Topics: Biology
    Notes: Summary We have examined the metamorphosis of the wing imaginal disc of Drosophila during culture in vitro in the continuous presence of 20-hydroxy ecdysone (0.1 μg/ ml). We find that the sequence of cellular changes in the wing blade during culture closely match those occurring in situ, involving two periods at which the dorsal and ventral surfaces are joined only by cell processes containing trans-alar microtubule arrays. Good pupal and imaginal cuticle secretion is found in this system.
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  • 154
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    Development genes and evolution 196 (1987), S. 222-230 
    ISSN: 1432-041X
    Keywords: Mesodermal cell lineages ; Cell transplantations ; Embryogenesis ; Drosophila
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We describe the results of cell transplantation experiments performed to investigate mesodermal lineages in Drosophila melanogaster, particularly the lineages of the somatic muscles, the visceral muscles and the fat body. Cells to be transplanted were labelled by injecting a mixture of horseradish peroxidase (HRP) and fluorescein-dextran (FITC) in wild-type embryos at the syncytial blastoderm stage. For transplantation cells were removed from the ventral furrow, 8–12 min after the start of gastrulation, and individually transplanted into homotopic or heterotopic locations of unlabelled wild-type hosts of the same age. HRP labelling in the resulting cell clones was demonstrated histochemically in the fully developed embryo; histotypes could be distinguished without ambiguity. Mesodermal cells were already found to be committed to mesodermal fates at the time of transplantation. They developed only into mesodermal derivatives and did not integrate in non-mesodermal organs upon heterotopical transplantation. No evidence was found for commitment to any particular mesodermal organ at the time of transplantation. The majority of somatic muscle clones contributed cells to only one segment. However, clones were not infrequently distributed through two or even three segments. Clones of fat body cells were generally restricted to a small region. However, cells of clones of visceral musculature were widely distributed. With respect to the proliferative abilities of transplanted cells the clones were difficult to interpret due to the syncytial character of the somatic musculature and the fact that the organization of the other organs is poorly understood. Evidence from histological observations of developing normal embryos indicates only three mitoses for mesodermal cells. Clones larger than seven cells were not found when embryos were fixed previous to germ-band shortening; larger clones were found in the fat body and visceral musculature after fixing the embryos at the end of organogenesis. Quantitative considerations suggest that a few mesodermal cells might perform more than three mitoses.
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  • 155
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    Development genes and evolution 196 (1987), S. 511-521 
    ISSN: 1432-041X
    Keywords: Segmentation defects, ether-induced ; Drosophila ; Phenocopies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Drosophila embryos, exposed to ether between 1 and 4 h after oviposition, develop defects ranging from the complete lack of segmentation to isolated gaps in single segments. Between these extremes are varying extents of incomplete and abnormal segmentation. On the basis of both their temporal and spatial characteristics, five major phenotype classes may be distinguished: headless — unsegmented or incompletely segmented anteriorly; gap — interruptions of segmentation not obviously periodic; alternating segment gaps — interruptions with double segment periodicities; fused segments; and short segments — truncations with single segment periodicities. Many defects resemble known mutant phenotypes. The disturbances in segmentation are predominantly global and frequently accompanied by alterations in segment specification, such that the segments obtained show no resemblance to the normal homologues. These features, together with the distinctive spatiotemporal characteristics of the defects, all point to segmentation as a dynamic process. The regular spacing of the segments and the fact that the entire range of defects is inducible by ether are further consistent with the hypothesis that at least part of the segmentation process may consist of physicochemical reactions coordinated over the whole body. The relationship between our data and data from genetic and other analyses are briefly discussed.
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  • 156
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    Development genes and evolution 196 (1987), S. 12-15 
    ISSN: 1432-041X
    Keywords: Gene expression ; Drosophila ; Mitochondria ; Temperature sensitivity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The fine structure of the indirect flight muscles was studied by electron microscopy in the following Notch locus mutants of Drosophila melanogaster reared at 18° C or 29° C for 6 days after eclosion: Ax 16172/Ax16172, Ax28/ Ax28, l(1)Nts1/l(1)Nts1,l(1)Nts1/Y and in wild-type controls. The flies were raised up to eclosion at 25° C or 18° C. It was observed that the l(1)Nts1 flies gradually became flightless within a few days if reared at 29° C as adults, and gross changes in the fine structure of the flight muscles were also observed in flies of this genotype. Peripheral myofilaments of myofibrils were disarranged and the mitochondria diminutive. At 18° C the flight muscles remained normal. In all of the Abruptex (Ax) combinations the flight muscles remained similar to the wild-type controls at both 18° C and 29° C, i.e. they were normal. The results suggest that the Notch gene is active in adult flies in addition to its activity during embryonic, larval and pupal stages, and is directly or indirectly involved in the adult development of the muscle tissue.
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  • 157
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    Keywords: Drosophila ; Spermatogenesis ; Y chromosome ; Lampbrush loops ; Sex determination ; Maternal effects
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    Topics: Biology
    Notes: Summary The Y chromosome of Drosophila hydei carries information that is necessary for the development of the spermatozoa. In primary spermatocytes Y chromosomal genes become active: five of the male fertility factors form giant lampbrush loops. Our prior work indicated interactions between the Y chromosomal genes and autosomal loci. It is of interest to identify loci regulating the activity of the Y chromosomal genes. We, therefore, screened a total of about 14,000 chromosomes (X, 2, 3 and 4) for mutations that interfere with the expression of the lampbrush loops. Two mutations with substantial effects on the loop morphology were recovered. One of them, a recessive male sterile mutation (ms (3) 5) on chromosome 3, is described in this paper. Its homozygous state results in a complete absence of all Y chromosomal lampbrush loops at 26° C; at 18° C the loops are formed. Temperature shifts with homozygous males indicate that the function early during the spermatogonial stage is crucial for the development of lampbrush loops in the primary spermatocyte. Meiosis is entirely absent in the male, but normal in females. Females homozygous for ms (3) 5 display a maternal effect, which reduces the viability and fertility of homozygous daughters and produces sons with signs of intersexuality. Linkage studies indicated that the effect on the male germ line and the maternal effects cannot be separated and may hence be induced by a single gene.
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  • 158
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    Keywords: Drosophila ; Collagen ; Haemocytes ; Basement membranes
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    Topics: Biology
    Notes: Summary We report a direct examination of the expression of one collagen gene (DCg1) during Drosophila melanogaster metamorphosis, based on data from in situ hybridization. The transcripts of this gene, thought to encode a basement membrane type IV collagen, are mainly accumulated during ecdysis in wandering haemocytes. Our results demonstrate that haemocytes contribute to extracellular matrix deposition and seem to perform a fibroblastic function during Drosophila development.
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  • 159
    ISSN: 1432-041X
    Keywords: Drosophila ; Homoetic ; Mutational analysis ; Transformation ; Distal disc structures
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    Topics: Biology
    Notes: Summary A gene Brista has been identified in chromosome 2R, in the region 60D11-E4, in which mutations cause homoeotic transformation of distal antennal structures to distal leg derivatives, and in which certain alleles also lead to upsets in the formation of distal elements of the legs. This gene is haploinsufficient for the homoeotic phenotype. Several putative null and two hypomorphic alleles have been recovered. The effects of exposure to the non-permissive temperature of a temperature-sensitive allele are cummulative and depend upon the length of the exposure during the period of antennal cell proliferation. It is suggested that this gene contributes to the stability of the state of determination in distal domain of the antennal and leg discs, and its relationship to other genes with similar mutant phenotype is discussed.
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  • 160
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    Development genes and evolution 196 (1987), S. 141-157 
    ISSN: 1432-041X
    Keywords: Drosophila ; Blastoderm fate-map ; Tail segmentation ; Larval cuticle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The segmental organisation of the tail region in the embryo of Drosophila melanogaster, which is defined here as the epidermal region posterior to the boundary between abdominal segments A7 and A8, has been investigated by means of ultraviolet (UV) laser fate-mapping and phenotypic analysis of embryonic mutants that alter the segmental pattern of the larval cuticle. Wild-type embryos were irradiated in the presumptive tail region with a UV- laser microbeam of 20 μm diameter at the blastoderm stage. The ensuing defects were scored in the cuticle pattern of the tail region of the first-instar larva, which is described in detail in this paper. The spatial distribution of defect frequencies was used to construct a blastoderm fate-map of the cuticle structures of the larval tail region. The segmental origin of the larval tail structures was inferred from the phenotypic analysis of segmentation and homoeotic mutants, which revealed pattern repetition throughout the embryonic tail region corresponding to four segment anlagen, A8 to A11, and a non-segmental telson. These data enabled the transformation of the blastoderm fate-map of cuticle structures into a map of tail segment anlagen. The tail anlage occupies about 10% of the egg length (EL), bounded by segment A7 anteriorly at 20% EL and by the proctodaeum posteriorly at 10% EL, as measured from the posterior pole. The anlagen of segments A8 and A9 appear to be narrow dorso-ventral strips of blastoderm cells similar to the anlagen of the trunk segments, whereas the anlagen of A10 and A11 are smaller and produce fewer pattern elements. The telson is represented in the cuticle by the tuft which derives from a very dorsal posterior position. The antero-posterior axis of the entire tail anlage appears curved upward posteriorly. Differences in the mode of development between tail and trunk segments are discussed, as are similarities of larval and imaginal tail development in Drosophila. Comparison with tail development in other insects suggests that, during evolution, the transition from semi-long-germ to long-germ development modified the organisation of the tail region without affecting its primary subdivision into metameric units.
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    Development genes and evolution 196 (1987), S. 101-112 
    ISSN: 1432-041X
    Keywords: Peripheral nervous system ; Compartments ; Segmentation mutants ; Drosophila
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The peripheral nervous system of embryos homozygous for prd, ftz, en and bxd was examined for defects and transformations in the segment-specific pattern of sensilla and peripheral nerves. This analysis permitted me to assign a distinct subset of sensilla to any of the three genetically and morphologically defined compartments s, a and p of each segment. In the wild-type embryonic segments, sensory axons deriving from sensilla of different compartments form a part of the common peripheral nerves. In the composite segments of prd and ftz mutant embryos, subsets of sensilla of two neighbouring segments are combined. Nevertheless, the axons of sensilla of different segmental identity are able to fasciculate and to form afferent nerves, which connect in an apparently normal fashion to the central nervous system. It is concluded that in the Drosophila embryo compartmental and segmental identity of sensory organs has no influence on the trajectories of sensory axons.
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  • 162
    ISSN: 1432-041X
    Keywords: Drosophila ; Dorsalizing mutant ; Phenotypic rescue ; Poly(A)+ RNA ; Cytoplasm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary spätzle (spz), a maternal effect gene of Drosophila, is involved in the establishment of the dorso-ventral axis during embryogenesis. Eggs from females lacking the spz gene product develop into completely dorsalized embryos, i.e. the ventral and lateral pattern elements fail to develop. Upon injection of either cytoplasm or poly(A)+ RNA from early wild-type embryos, spz embryos develop lateral pattern elements represented by Filzkörper and in the case of injected cytoplasm additional ventral pattern elements represented by ventral setae. Wild-type cytoplasm retains the rescuing activity longer than the poly(A)+ RNA fraction does, and cytoplasm is always more effective in provoking the rescue than poly(A)+ RNA. Mosaic females containing spz germ cells surrounded by spz + tissues were generated by pole cell transplantations; a mutant genotype in the germ cells is sufficient to produce all aspects of the spz mutant phenotype, suggesting that the maternal source of spz gene product is the germ line.
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  • 163
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    Cellular and molecular life sciences 43 (1987), S. 213-215 
    ISSN: 1420-9071
    Keywords: Drosophila ; reproductive behavior ; oviposition ; pheromones
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A residual influence of males and females on oviposition has been examined in 7 drosophilids. There was evidence for oviposition deterrence inDrosophila funebris, with males as well as females producing the inhibitory effect. In contrast, male residues stimulated oviposition inZaprionus tuberculatus. Male residues also stimulated oviposition and appeared to serve as an aggregation cue inD. melanogaster.
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  • 164
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    Cellular and molecular life sciences 43 (1987), S. 931-933 
    ISSN: 1420-9071
    Keywords: Hyperthermia ; gamma ray ; crossing-over ; Drosophila ; repair mutant
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Hyperthermia of 1 h at 38°C did increase gamma-ray induced crossing-over in meiotic cells of male larvae and adults. However, there was considerably less effect of the heat treatment upon radiation induced crossing-over (a chromosome breakage event) in an excision repair mutanty mei-9 a.
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  • 165
    ISSN: 1432-1939
    Keywords: Drosophila ; Polymorphism ; Stenocereus ; Host-plant-shift ; Developmental homeostasis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Chromosomally polymorphic populations of Drosophila mojavensis from Baja California feed and breed on agria cactus, Stenocereus gummosus; whereas, monomorphic Arizona populations are associated exclusively with organ pipe cactus, S. thurberi. The effects of this host plant shift in expanding the kinds of feeding and breeding sites were assessed by manipulating larval density and recording differences in egg to adult development time and viability, and adult thorax size in both populations on artificially rotted substrates of both cactus species. Older agria rots increased development time but had no effect on viability. Organ pipe rots were qualitatively poorer substrates than agria rots for both monomorphic and polymorphic populations of D. mojavensis, especially at higher larval densities causing longer egg to adult development times, lower viabilities, and smaller thorax sizes than agria. The Baja population expressed shorter development times, higher viabilities, and smaller thorax sizes than the Arizona population on both cactus substrates. No evidence for cactus host race formation was found. The Baja population was less sensitive to increasing larval densities for all fitness characters studied on both cactus substrates indicating greater developmental homeostasis than in the monomorphic Arizona population. These data support the hypothesized central-marginal population structure within this species coincident with the distribution of host plants and lend insight into the process of adaptive divergence at different life history stages caused by host plant shifts.
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  • 166
    ISSN: 1573-4927
    Keywords: mutagenesis ; alcohol dehydrogenase ; formaldehyde ; Drosophila ; deletions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Adh fn23 andAdh fn24 are two formaldehyde-induced, homozygous-viable, alcohol dehydrogenase-null mutants that bear lesions in the gene tht codes for the alcohol dehydrogenase (ADH; EC 1.1.1.1) ofDrosophila melanogaster. Adh fn23 contains a 34-base pair deletion in the C-terminal coding region of the alcohol dehydrogenase structural gene. By immunological and molecular analysis, we show that the deletion shifts the translation reading frame and results in a prematurely truncated polypeptide product (10 amino acids shorter than wild type) that cross-reacts with antibody raised against ADH. The steady-state level of alcohol dehydrogenase mRNA present in this mutant is close (97%) to that in the wild type, but the steady-state level of alcohol dehydrogenase-like protein is 50% lower. Moreover, the rate of alcohol dehydrogenase synthesis inAdh fn23 flies is reduced to 60% of that found in the wild type. Hence both the rate of synthesis and the rate of degradation of alcohol dehydrogenase are affected. In contrast,Adh fn24 which contains an 11-base pair deletion in the N-terminal coding region of the ADH gene, synthesizes no immunodetectable protein, and the amount of alcohol dehydrogenase mRNA is less than half that of wild-type flies. As withAdh fn23, the deletion inAdh fn24 results in a change in the reading frame. UnlikeAdh fn23, however, nucleic acid sequence data indicate that polypeptide chain elongation can proceed for a considerable distance (over 130 amino acids) beyond the deletion. Based upon antigenic binding-site predictions, the resultant aberrant protein (projected 195 amino acids in length) would share few antigenic sites with the alcohol dehydrogenase from the wild type, which may account for the lack of immunoprecipitable material in this mutant. The contrasting effects these two deletions have on theDrosophila ADH mRNA levels and ADH protein levels are discussed.
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    Journal of chemical ecology 13 (1987), S. 2069-2081 
    ISSN: 1573-1561
    Keywords: Drosophila ; D. mojavensis ; D. nigrospiracula ; D. mettleri ; Diptera ; Drosophilidae ; cactus ; alkaloids ; viability ; development ; longevity ; host-plant relationships
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Drosophila mettleri is a soil-breeding, cactophilic drosophilid which lives in the Sonoran Desert. Several chemical constituents of cacti in this region have been identified as having major roles in insect-host plant relationships involvingDrosophila. For example, isoquinoline alkaloids, which are present in senita cactus, have been shown to be toxic to seven of the nine species tested. The two tolerant species areD. pachea, the normal resident, andD. mettleri. Necroses of senita cacti are often used as feeding substrates byD. mettleri adults, but this species has never been reared from senita rots. Soil, which have been soaked by juice from saguaro and cardón rots, are the typical breeding substrates of this species. The tissues of both of these cacti also contain alkaloids, chemically related to those in senita, but at much lower concentrations. Alkaloid concentration in saguaro-soaked soil was found to be 1.4–27 times the average concentration in fresh tissue. Alkaloids were extracted from saguaro tissue and used in tests of larva-to-adult viability, developmental rate, and adult longevity. Elevated concentrations of saguaro alkaloids had no significant effect on the longevity ofD. mettleri, but significantly reduced the longevity ofD. nigrospiracula andD. mojavensis, two nonsoil breeding cactophilic species. Viability and developmental rates of all three species were affected, but the effect onD. nigrospiracula was comparatively greater. It is argued that the adaptations that allowD. mettleri to utilize the saguaro soil niche also convey tolerance to alkaloids present in senita tissue. The ability to utilize senita necroses as feeding substrates represents an ecological advantage to D. mettleri, in that the density of potential feeding sites is increased as compared to species which are more specific in their host-plant relationships.
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    Behavior genetics 17 (1987), S. 541-558 
    ISSN: 1573-3297
    Keywords: mating ability ; sexual selection ; fitness ; stress ; domestication ; Drosophila
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Psychology
    Notes: Abstract Mating ability differences between flies of different alcohol dehydrogenase (Adh) genotypes have been assessed in the temperature range 15 to 29°C for laboratory-adapted and field-derivedDrosophila melanogaster. Significant differences amongAdh genotypes were detected principally for the laboratory-adapted strains due to departures from random mating associated with heterozygote superiority at the relatively extreme temperature of 29°C, although mating ability differences could not be attributed directly to theAdh locus. The difference between the laboratory and the field populations can be explained by the effects of genetic back-ground manifested in the form of fitness differences, being enhanced for the laboratory-adapted flies as a consequence of the stress of laboratory culture. In contrast with larval survival and development time, laboratory and field flies do not differe appreciably in their overall abilities to obtain mates, which indicates that mating ability is a direct fitness character not greatly affected by laboratory culture. It follows that direct fitness traits are the least amenable to change under domestication.
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    Behavior genetics 17 (1987), S. 559-569 
    ISSN: 1573-3297
    Keywords: sexual selection ; Drosophila
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Psychology
    Notes: Abstract Since Darwin's formulation of the theory of sexual selection a number of population biologists have developed models that explore the genetic consequences of his theory. In all these models it is assumed that two forces act to counterbalance the runaway process of sexual selection. That is, female preference for a certain male character tends to select for extreme forms of that character until natural selection exerts its forces to maintain the optimum male phenotype that is able to survive in its environment. In this paper, an alternative explanation for the origin of secondary sexual characters is proposed. It is suggested that polymorphism in secondary sexual characters may be maintained not as a direct result of selection for these characters but by being either linked to or as pleiotropic effects of some other feature of the mate recognition pattern. While there are no genetic data to support these observations inDrosophila at this time, there appears to be compelling evidence that mating success is not wholly dependent on the presence of these characters.
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  • 170
    ISSN: 1573-4927
    Keywords: Drosophila ; vermilion ; tryptophan oxygenase ; suppression ; epigenetic suppression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The suppressor gene,su(s)2, inDrosophila melanogaster restores the production of red and brown eye pigments for some purple and vermilion mutant alleles, respectively. We showed previously that the product of thesu(s)+ allele caused inhibition of the sepiapterin synthase A produced by the purple mutant but did not affect the wild-type enzyme. Suppression was accomplished by removingsu(s)+ from the genome. We now report that the tryptophan oxygenase, produced by suppressible vermilion alleles, is also inhibited by extracts fromsu(s)+ flies. The inhibition of the vermilion enzyme can be reduced or eliminated, respectively, by prior storage of the extract at 4 or −20°C or by boiling, whereas the wild-type enzyme is not affected by extracts ofsu(s)+ flies. Also, when the suppressible vermilion strain is raised on certain diets, brown eye pigment production occurs. This epigenetic suppression was reduced by the presence of an extra copy ofsu(s)+ in the genome. These data support a posttranslational mechanism for regulation of enzyme activity in which the activity of the mutant enzyme is reduced by the product of thesu(s)+ allele. How thesu(s)+ gene product can distinguish between the normal and the mutant forms of these two enzymes is discussed, along with other mechanisms for suppression that are currently under investigation.
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  • 171
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    Biochemical genetics 25 (1987), S. 591-602 
    ISSN: 1573-4927
    Keywords: trypsin inhibitor ; homoeoloci ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The aneuploids of Chinese Spring wheat have been used to locate the genes(Ti-2) coding for a novel series of trypsin inhibitors to the long arms of the homoeologous group 5 chromosomes. Three allelic variants at the 5D locus were detected in a limited survey among wheat varieties, but no variation at the loci on either chromosome 5A or chromosome 5B was detected. Homoeoloci were found in a number of alien relatives, and in the majority of cases, these were present on the group 5 homoeologue. However, inAegilops umbellulata, theTi-U2 locus was located on a chromosome presumed to belong to homoeologous group 1. NoHordeum vulgare orH. chilense Ti-2 gene was expressed in a wheat background. This new marker will be especially useful as a screening mechanism for nullisomy of chromosome 5B in work aimed at introgression of alien chromatin into wheat.
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  • 172
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    Behavior genetics 17 (1987), S. 597-611 
    ISSN: 1573-3297
    Keywords: sexual selection ; sexual isolation ; Drosophila
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Psychology
    Notes: Abstract The idea that sexual selection is responsible for most of the characters, morphological, physiological, and behavioral, that are observed as subserving the efficiency of the reproductive act as an important monitor of fitness is developed. As a corollary, sexual isolation is downgraded, being considered a relatively unimportant secondary process for which the term “mechanism” is singularly inappropriate. The reproductive isolation frequently observed between allopatric species appears to me to be mostly an incidental out come of the fine tuning of the intrapopulational efficiency of the process of sexual reproduction. Two points are stressed: first, sexual selection is a powerful means of serving fitness; and second, hybridization poses little threat to the integrity or future well-being of a species.
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  • 173
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    Molecular biology reports 12 (1987), S. 79-83 
    ISSN: 1573-4978
    Keywords: Drosophila ; 20-hydroxyecdysone ; puromycin ; protein synthesis ; cycloheximide ; imaginal wing disc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of cycloheximide and puromycin on 20-hydroxyecdysone-induced protein synthesis in wing discs of Drosophila melanogaster has been studied by one-dimensional and two-dimensional SDS polyacrylamide electrophoresis. It is found that puromycin, but not cycloheximide, when applied simultaneously with the hormone enhanced the hormone-induced synthesis of the ‘early’ and ‘late’ proteins. However, when puromycin was applied after hormone treatment, only the ‘late’ proteins were induced. The possible implication of these observations is discussed.
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  • 174
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    Bioscience reports 7 (1987), S. 239-246 
    ISSN: 1573-4935
    Keywords: antisense RNA ; hsp 23 ; Drosophila
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The specificity of action of antisense RNA for one ofDrosophila low molecular weight heat shock proteins (hsp 23) was tested at the translational level using the rabbit reticulocyte lysate cell-free system. T7 polymerase-driven transcripts of hsp 23 in the antisense orientation were mixed with mRNA from heat-shocked cells under various stringency conditions prior to translationin vitro. Although the four small hsps show considerable sequence homology in their coding sequences, antisense hsp 23 RNA was shown to specifically inhibit hsp 23 mRNA translation under both high (formamide, 45°C and low stringency (37°C conditions. This suggests that the 5′ leader and the ribosome binding region of mRNA are of prime importance in the specificity of action of antisense RNA at the translational level.
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  • 175
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    Molecular genetics and genomics 210 (1987), S. 557-563 
    ISSN: 1617-4623
    Keywords: Drosophila ; Bithorax ; Transvection ; cis interaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have studied the influence of some mutations in the bithorax complex on the observed synapsis dependent phenotype of the genotypes Cbx 1Ubx1/+ and bx 34e/Ubx1. The effect of these mutations is similar to that introduced by disruption of pairing or by the z a mutation. Among the bx mutations, we find that bx 8 behaves differently from most other bx mutations in its influence on the synapsis dependent phenotype. This observation induced us to map the position of bx 8 with respect to other bx mutations; we find that it maps between bx 34e and bx 3. We show how some of the observations reported here can be fitted into a model of activation of the bithorax complex proposed by one of us.
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  • 176
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    Molecular genetics and genomics 208 (1987), S. 226-229 
    ISSN: 1617-4623
    Keywords: Drosophila ; Autosomes ; Mutagenesis ; Compound chromosomes ; Temperature-sensitive
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Mutants of Drosophila melanogaster are being used increasingly for studying different biological mechanisms. However, most attempts to identify new mutations have been restricted to the X-chromosome. It has been very difficult to identify new loci on the autosomes, as recessive mutations have to be made homozygous by setting up independent cultures for each mutagenized chromosome. We introduce a mutagenesis scheme which does not require setting up independent cultures. It uses meiotic recombination in compound autosomes to make recessive mutations homozygous and allows the screening of tens of thousands of mutagenized chromosomes with relatively little effort. In a pilot experiment, we tested about 33,300 chromosomes for temperature-sensitive paralytic mutations. We obtained 62 independent paralytic mutations and a large number of other mutations. Eight out of 25 of the paralytic mutations are on the autosomes. This method makes autosomes, which constitute about 80% of the Drosophila genome, more accessible for mutational analysis of various biological mechanisms.
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  • 177
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    Molecular genetics and genomics 210 (1987), S. 407-412 
    ISSN: 1617-4623
    Keywords: Drosophila ; Embryogenesis ; Heat shock ; protein synthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Very short heat shocks are administered to carefully staged early embryos of Drosophila melanogaster, and the effects on protein synthesis pattern investigated. A shock as short as 2 min will induce the heat shock response (reduction of normal protein synthesis, increased synthesis of the heat shock proteins) in syncytial blastoderm or later stages. Thus the initial events of the heat shock response must occur within 2 min, and not reverse upon rapid return to 22° C. A low level of synthesis of the 70 kDa heat shock protein is sometimes visible in unshocked animals, but may be induced by the labeling procedure. Survival following a short shock is not strictly correlated with a high level of heat shock response. Pre-blastoderm embryos do not produce significant heat shock protein, but survive a 2 min 43°C heat shock better than do heat shock response competent blastoderm embryos. The protein synthesis pattern prior to the blastoderm stage is very stable, possibly enhancing survival following a short shock. Shocks of 3 min or longer are more detrimental to pre-blastoderm embryos than to later stages, confirming the role of the heat shock response in survival following a longer shock. Stage-specific developmental defects (phenocopies) may be induced by heat shock at the blastoderm or later stages. Induction of these defects may require disruption of the normal protein synthesis pattern. Use of very short heat shocks to induce the heat shock response will be valuable in identifying the precise time at which a specific defect can be induced.
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  • 178
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    Molecular genetics and genomics 209 (1987), S. 290-298 
    ISSN: 1617-4623
    Keywords: Drosophila ; Gene structure ; Mutation ; 3′ processing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The Cs gene lies between the functionally and evolutionarily related dopa decarboxylase (Ddc) and l(2) amd loci of Drosophila. The Cs and Ddc genes overlap at their 3′ ends, implying that the transcription termination signals of these genes are polar, since each gene's primary transcript contains the complement of the other gene's transcription termination signals. The mature transcripts of the Cs and Ddc genes are complementary for a short distance and the primary transcripts may be complementary over thousands of base pairs. Despite intensive mutagenesis in this region, no mutations affecting the Cs transcript have been recovered although over 90 alleles of the two flanking genes (Ddc and l(2) amd) have been identified. Unlike the flanking Ddc and l(2) amd genes, the structure of the Cs gene and the temporal and tissue specificity of Cs expression are inconsistent with any structural or functional relatedness to the Ddc gene family. The internal structure of the Cs transcript is unlike that of most protein coding genes; it contains several open reading frames which are not situated favorably for efficient translation of the Cs message. This unusual internal structure may be the basis of the observed mutational silence of the Cs locus.
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  • 179
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    Behavior genetics 17 (1987), S. 307-312 
    ISSN: 1573-3297
    Keywords: Drosophila ; D. melanogaster ; olfaction ; ethanol tolerance ; correlated response
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Psychology
    Notes: Abstract The experiments usedDrosophila melanogaster lines previously selected for increased knockdown resistance to ethanol. Selected lines utilized ethanol as a metabolic resource to a greater extent than unselected lines. Lines were characterized by their olfactory responses to ethanol, ethyl acetate, and acetaldehyde in a wind tunnel. Selected lines were less attracted to ethanol than unselected lines but did not differ consistently in their responses to other chemicals. This suggests that increased tolerance and utilization of ethanol are not necessarily accompanied by increased attraction to this chemical.
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  • 180
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    Behavior genetics 17 (1987), S. 409-425 
    ISSN: 1573-3297
    Keywords: effect of isolation on mating ; rare-male mating advantage ; rare-female mating advantage ; artifact ; bias ; size of mating chamber ; Drosophila
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Psychology
    Notes: Abstract Virgin males (or females) of some species ofDrosophila, when stored singly, are known to be superior in mating to males (or females) stored in groups. This may create a spurious rare-male effect on some occasions. When no account is taken of this storage effect in an experimental setup designed to show rare-male mating advantage, bias in favor of a raremale effect may result. It is shown that merely by storing the rare males singly and the common males in groups, with males not differing in any other respect, a very strong spurious rare-male effect can be produced. Similarly, it is shown that a spurious rare-female effect is possible too. It is proposed that the very strong rare-male effect for pepperment scent as found by Dal Molin [(1979).Am. Nat. 113:951–954] is merely a result of such a bias. The relevance for natural populations of the mating advantage associated with the single housing condition is discussed. In the experiments designed to show a spurious rare-male effect, mating chambers of two different sizes were used. It is shown that sexual selection is more severe in the small chambers, for both males and females.
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  • 181
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    Molecular genetics and genomics 207 (1987), S. 374-384 
    ISSN: 1617-4623
    Keywords: master mind ; Molecular cloning ; Drosophila ; Neurogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The gene master mind (mam) is located in bands 50C23-D1 of the second chromosome of Drosophila melanogaster. mam is one of the neurogenic genes, whose function is necessary for a normal segregation of neural and epidermal lineages during embryonic development. Loss of function of any of the neurogenic genes results in a mis-routeing into neurogenesis of cells that normally would have given rise to epidermis. We describe here the molecular cloning of 198 kb of genomic DNA containing the mam gene. Ten different mam mutations (point mutants and chromosomal aberrations) have been mapped within 45 kb of the genomic walk. One of the mutations, an insertion of a P-element, was originally recovered from a dysgenic cross. Four different wild-type revertants of this mutation were characterized at the molecular level and, although modifications of the insertions were found, in no case was the transposon completely excised. An unusually high number of the repetitive opa sequence, and of an additional previously unknown element, which we have called N repeat, are scattered throughout the 45 kb where the mam mutations map. The functional significance of these repeats is unknown.
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  • 182
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    Molecular genetics and genomics 209 (1987), S. 360-365 
    ISSN: 1617-4623
    Keywords: Drosophila ; Yolk protein ; protein processing ; secretion ; female-sterile mutant ; DNA sequencing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The three yolk proteins (YP1, YP2 and YP3) of Drosophila melanogaster are synthesised in the fat body and ovarian follicle cells and selectively accumulated in the developing oocytes to provide a nutrient source for embryogenesis. We have described the phenotype of a temperaturesensitive female-sterile mutant, fs(1) K313, and characterised its yolk proteins. This mutation affects the secretion of YP2 and is the first mutation affecting YP2 to be described. Using genetic and molecular tests we argue that the female-sterile phenotype results, at least in part, from the abnormal secretion of YP2 perturbing the follicle cell secretory pathway in general and thus causing defects in chorion protein secretion. The gene coding for YP2 in fs (1) K313 has been cloned and sequenced. Two amino acid substitutions have been found which probably cause the abnormal secretion of YP2 and the resulting female-sterile phenotype.
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  • 183
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; electrophoresis system ; acid polyacrylamide gel ; gliadins ; variety identification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In this paper we describe the method of wheat gliadin electrophoresis in use at RIVRO, Wageningen. It differs from other techniques mainly by the application of an alternative buffer system, making it possible to polymerise the gels in a buffered alkaline environment and to perform the run at pH 3.1 without extensive buffer changing steps. Advantages are a greater gel reproducibility and the ease of gel handling. Furthermore, a rationalised protein extraction procedure, a cheap shaking system for staining baths and a better (slower moving) tracking dye are described.
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  • 184
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Hordeum bulbosum ; crossability ; timing of pollination ; frequency of fertilization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effect of manipulating the timing of pollination on the fertilization frequency in wheat × tetraploid Hordeum bulbosum hybridizations was investigated as a possible means of overcoming varietal incompatibility in this cross. Pre- and post-anthesis pollinations were made on previously identified highly crossable and poorly crossable wheat genotypes and the stage of development of the individual florets at pollination was expressed in ‘days from anthesis’ units. In both crossable and non-crossable wheat genotypes, a significant negative linear relationship was observed between floret age and fertilization frequency. The magnitude of the response of early pollination was dependent on the number of alleles for non-crossability present at the Kr loci. The non-crossable cultivar Highbury, possessing more than one allele for incompatibility, showed a slight response. The greatest response was shown by the highly crossable cultivar Chinese Spring, possessing alleles for crossability at all Kr loci. The single chromosome substitution line, Chinese Spring (Hope 5B), possessing the most potent allele for non-crossability, Kr 1, showed an intermediate response. The H. bulbosum genotype had no effect on the magnitude of this response, influencing only the mean fertilization frequency.
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  • 185
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Hordeum bulbosum ; pollen grain germination ; pollen tube growth ; frequency of fertilization ; gibberellic acid ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A quantitative examination of pollen grain germination and pollen tube growth within the overy wall was made in selected wheat × tetraploid H. bulbosum hybridizations, to investigate the effect of the wheat and the H. bulbosum genotype on these characters. As expected, variation at the known crossability loci had no effect on pollen grain germination. The frequency of pollen tube penetration of the ovary wall was, however, severely reduced when the dominant alleles were present. Pollen tube penetration was nevertheless observed in 3 of the 110 ovaries examined of the non-crossable cultivar Highbury. The H. bulbosum genotype had a much smaller effect on these characters, but significant differences between the clones were observed in the numbers of pollen tubes initially penetrating the ovary wall. Although two H. bulbosum genotypes showed no significant differences in the number of ovaries with pollen tubes at the base of the ovule, significant differences in the frequency of fertilization were observed. The possible cause of this discrepancy is discussed. The frequency of fertilization in crossable wheat × H. bulbosum hybridizations was improved by the application of gibberellic acid within 10 minutes of pollination, and reduced by an increase in the ambient temperature from 20°C to 26°C. Fertilization following the pollination of non-crossable wheat genotypes was not affected by either of these factors.
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  • 186
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Hordeum bulbosum ; frequency of fertilization ; seed set ; seed survival ; gibberellic acid ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary An investigation was made of factors affecting the frequency of development of fertilized ovules following wheat x Hordeum bulbosum hybridizations. Seed survival, a term used here to represent the relationship between seed set and fertilization, was shown to vary between the wheat genotypes tested, but was unrelated to the crossability genes present within the wheat genotype. A high seed survival was obtained in crosses involving the variety TH3929 and the single chromosome substitution line, Chinese Spring (Hope 5B). Chinese Spring showed a poor seed survival. Some influence of the H. bulbosum genotype on seed survival was detected. The two environmental factors studied, namely the post-pollination application of gibberellic acid and the ambient temperature, were both shown to affect seed survival. The application of gibberellic acid immediately after pollination or 1 and 2 days after pollination improved seed survival, although the extent of the response was dependent upon the number and timing of the application(s). A genotype-dependent response to the ambient temperature was observed, TH3929 showing a slight but insignificant reduction and Chinese Spring a significant improvement in seed survival with an increase in temperature from 20°C to 26°C. The physiological processes which may be influenced by GA and the ambient temperature and may thereby affect seed survival are discussed.
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  • 187
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    Euphytica 36 (1987), S. 831-839 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; honeycomb selection ; breeding methodology ; selection intensity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effectiveness of the honeycomb selection method for yield in spring wheat (Triticum aestivum L.) was evaluated using progenies from two wheat crosses, Glenlea x NB131 and Glenlea x Era. Honeycomb selection was carried out in the F2 and F3 generations, grown at the University of Manitoba in the summers of 1980 and 1981, respectively. In both generations, divergent selection was made for both high and low yield. Plants selected in the F3 generation were entered in an F4 yield test in the summer of 1982. Results of the experiment showed that honeycomb selection for yield in the F2 and F3 generations was effective in identifying parents of high- and low-yielding lines. F3 plants from highyielding F2 selections gave higher yields than those from low-yielding F2 selections by 11.5% and 13.0% for Glenlea x NB131 and Glenlea x Era crosses, respectively. The F4 yield test showed that high yielding selections from both crosses significantly outyielded by 8.9% low yielding selections and by 14.4% the unselected composite lines. It is concluded that the honeycomb selection method can be used for early generation selection in spring wheat.
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  • 188
    ISSN: 1573-5028
    Keywords: wheat ; maize ; bean mitochondria ; tRNAPro (UGG) ; gene localization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The four Sal I fragments of wheat mitochondrial DNA containing the 18S and 5S ribosomal RNA genes were screened for the presence of tRNA genes. Upon sequencing, a tRNAPro (UGG) gene was found in two of these four fragments. The localization of the corresponding gene on the maize mitochondrial genome was established. Transcriptional studies have shown that this gene is transcribed in wheat and maize mitochondria. The sequence of the corresponding tRNAPro (UGG) of bean mitochondria was determined using in vitro post-labeling techniques.
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    Plant and soil 101 (1987), S. 51-60 
    ISSN: 1573-5036
    Keywords: dry matter ; field capacity ; growth function ; nitrogen uptake ; rooting ; sowing date ; thermal time ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The quantities,Y, of nitrogen taken up, and dry matter produced, at various times during the growth of six winter wheat crops at Rothamsted were shown to be related to thermal time,x, based on soil temperature, by a simple equation $$Y = (A^{ - 1/n} + \exp ( - kx))^{ - n} $$ whereA is the ultimate maximum ofY, n a shape factor andk a rate constant that is related toA andn throughx′, the inflexion point of the function. The value ofn was 1.5 for both N uptake and dry matter. The value ofA for N uptake,A N , was well described by a multiple regression on sowing data,t s , expressed as the number of days after August 31st, and rainfall,R Apr , in the April before harvest, but no such regression could be found for the value ofA for dry matter,A D . The rate constants,k N andk D , for N-uptake and dry-matter production respectively, could be related to the date of sowing and the weather through the corresponding inflexion points,x′ N andx′ D . Highly significant regressions were found, forx′ N on the time,t sf , between sowing and the return of the soil to field capacity and forx′ D on the reciprocal oft s . The function was used to generate N uptake curves from values ofA N andk N (obtained fromx′ N ) given by inserting the appropriate values ofR Apr ,t s andt sf in the regressions. These fitted measured N uptakes satisfactorily for the six crops used to obtain the regressions, and four grown subsequently, at Rothamsted, and also for six crops at Woburn. Values ofA D had to be set arbitrarily because no regression had been found to predict them, but using these arbitrary values in the function gave dry matter curves that fitted the measurements satisfactorily for all ten Rothamsted crops and two of the Woburn crops. Tests for seasonal and site effects showed thatA N was influenced more by differences between the two sites than by seasonal differences other than those inR Apr , whereasA D was strongly influenced by these seasonal differencess and very little by those between the sites.
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  • 190
    ISSN: 1573-5036
    Keywords: barley ; Cochliobolus sativus ; common root rot ; subcrown internode ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The progression of common root-rot lesions on subcrown internodes of Neepawa wheat and Bonanza barley plants inoculated 14, 26, 38 and 50 days after seeding was measured in growth chamber tests. Both in wheat and barley, lesion development was more rapid in older than in younger plants. Variables such as mean daily rates of linear progression of lesions, disease ratings, and proportion of plants becoming severely diseased were higher in older than in younger plants. The possible stress resulting from the removal of the crown roots on more rapid disease development in older than in younger plants is discussed.
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  • 191
    ISSN: 1573-5036
    Keywords: amino acids ; salinity ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A lysimeter study was conducted on Cajeme wheat (Triticum aestivum L.) to investigate the impact of salinity on protein and free amino acid content of the grain. Cross correlations were obtained between 16 different soil-plant-water based parameters and the concentration and total accumulation of amino acids. The results indicated that after 3 years of irrigation, the majority of protein bound and free amino acids increased in concentration in the grain. However, both free tryptophan and free proline revealed decreasing concentrations with increasing salinity. Free tryptophan showed a synergism between total accumulation, yield and concentration. Free proline concentrations decreased in association with increasing protein concentrations. Cross correlations of the 16 soil-plant-water based parameters with free and protein bound amino acids revealed significant correlations for free aspartic acid and glycine with total accumulation but not with concentrations. Only methionine plus cystine was lower than suggested FAO levels for essential amino acids and was lower in the third year than in the first year.
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  • 192
    ISSN: 1573-5036
    Keywords: copper ; iron ; manganese ; Phaseolus vulgaris ; phosphorus ; VAM ; wheat ; zinc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effect of vesicular-arbuscular mycorrhizal fungi (VAM) on field bean and spring wheat dry matter production and on phosphorus, zinc, copper, iron and manganese uptake was determined under greenhouse conditions. Nutrient availability was varied by using different sizes of pots and by diluting the soil with sand. VAM increased plant dry matter production under all sets of growth conditions. VAM were found to directly increase the uptake of P, Zn, Cu and Fe by field beans and of P and Zn for wheat in both experiments. Increased uptake of the other nutrients measured was attributed to increased dry matter production or other factors. The effect of VAM decreased as the pot size holding the host plants decreased, but was not affected by the ratio of soil to sand if the pot size was kept constant. Nutrient uptake by beans as a proportion of total amount of nutrient present increased as the amount of nutrient decreased. Increase in root-soil contact and altered chemical equilibria are probable reasons for increased efficiency of nutrient uptake by beans as the level of available nutrient decreased. For wheat, which has a relatively fibrous root system, decreasing the nutrient availability had minimal effects on nutrient uptake in these experiments. Increases in total uptake of a particular nutrient resulting from inoculation with VAM are not necesarily indicative of a direct uptake of that nutrient by the VAM.
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  • 193
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    Plant and soil 104 (1987), S. 253-262 
    ISSN: 1573-5036
    Keywords: aluminium tolerance ; lime ; phosphate ; potassium ; root growth ; subsoil acidity ; translocation ; varietal differences ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Two wheat varieties with differing aluminium tolerance were grown in pots of acid soil. Liming did not change significantly the amounts of chemically extractable P and K, but caused improved vegetative growth, increased inflow of P and K and reduced uptake of Al. Without lime, roots had a higher content and concentration of P than shoots; liming reversed this. Without lime the sensitive variety with a shorter root length had an Al inflow ten times that of the tolerant one: tolerance involves a mechanism for exlcuding Al. The inflow of P per unit inflow of Al (mol ratio) without lime was three times greater for the tolerant variety which therefore has more P to counteract the effects of Al. The same varieties were grown in two-layer soil columns, with a low P status and a limed topsoil and acid subsoil. Liming the subsoil improved plant growth but this was still restricted by low P availability. Addition of P to the topsoil caused good growth regardless of subsoil acidity: root growth increased in both layers and P (labelled with32P) taken up from the topsoil was translocated to roots in the subsoil. This P inactivated root Al and allowed the roots to grow and take up more P from the acid subsoil with however a reduction in inflow. The sensitive variety was affected more by the acid subsoil and low P availability, had a similar ability to translocate P to subsoil roots but could not attain the growth rate of the tolerant wheat even with P and lime.
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  • 194
    ISSN: 1573-5036
    Keywords: competition ; nitrogen ; nutrition ; phosphorus ; wheat ; wild oats
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In the cultivated wheat fields of the Mediterranean area, wild oats (Avena sterilis L.), is commonly found a weed, which competes with the wheat for soil nutrients, water and light. To study this mutual competition for N and P, a pot experiment was devised in which the planting of wheat and weed seeds, in the pots, was so arranged that the lengths of time between the emergence of each specie was different for each treatment. When the time of emergence of the wild oat coincided with that of wheat, the latter was affected in its development, productivity and total accumulation of N and P. However, the earlier the emergence of wheat was relative to that of wild oat, the greater was the suppression of the weed, with a lower total accumulation of N and lower P content in the plant. Bringing forward the emergence of either of the two species conferred advantages for the development of the earliest emerger.
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  • 195
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    Euphytica 36 (1987), S. 221-224 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; auricle color ; compact head ; stem rust resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Previous evidence suggested that the gene for red auricle (Ra) might be linked to the gene Sr6 for stem rust resistance on chromosome 2D. However, tests indicated that Ra is not linked to either Sr6 or C, which is also on 2D.
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  • 196
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    Euphytica 36 (1987), S. 513-519 
    ISSN: 1573-5060
    Keywords: Tricticum aestivum ; wheat ; genetic analysis ; vernalization inhibiting genes ; Vrn genotypes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A study of the Vrn genotypes of 642 spring wheats supports the theory that only Vrn1, Vrn2 and Vrn3 exist in Tricticum aestivum. In none of the varieties investigated Vrn4 was present. Seven varieties, which according to literature carry Vrn4, showed to carry Vrn1, Vrn2 and/or Vrn3. Some varieties were mixtures of Vrn-genotypes, which could mislead geneticists in pooled data analysis. Other causes for misinterpretation of the data could be hybrid necrosis, hybrid dwarfness or a wrong determination of plants with a winter habitus. Only Hope was dominant on another Vrn locus. Its haploid Vrn-genotype is Vrn1 vrn2vrn3 Vrn5.
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  • 197
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    Plant growth regulation 5 (1987), S. 235-239 
    ISSN: 1573-5087
    Keywords: Triticum turgidum ; wheat ; Chlormequat ; CCC ; dwarfing genes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Two crosses between Triticum turgidum wheat lines differing in their response to chlormequat (CCC) were tested. In the F2 population of one cross, which was segregating for the Rht1 dwarfing allele, each plant was cloned by separation of two tillers, one of which was treated with CCC. The tall (rht1/rht1) and the intermediate (Rht1/rht1) genotypes showed a greater response to CCC than the semi-dwarf (Rht1/Rht1) genotype, as expressed by culm length and date of ear emergence. The F3 families of another cross and their two semi-dwarf parents were grown in a three-replicated field test in paris of rows, one of which was treated with CCC. In one of the parents and in 1/4 of the F3 families CCC induced a wide-angled tiller growth, suggesting a monogenic control of this growth habit in response to CCC.
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  • 198
    ISSN: 1573-5044
    Keywords: wheat ; somatic embryogenicis ; embryogenic callus ; 3,6-dichloro-o-anisic acid ; 2,4-dichlorophenoxyacetic acid ; 6-furfurylaminopurine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Nine experiments were conducted to determine effects of various culture medium addenda on inducation of embryogenic calli from immature embryos of a responsive Triticum aestivum L. genotype (PCYT 10). Effects were quantified by counting somatic embryos (embryoids) per callus. Optimal auxin concentrations to induce and maintain somatic embryogenesis were 3.62 μM 2,4-dichlorophenoxyacetic acid (2,4-D) or 9.05 μM 3,6-dichloro-o-anisic acid (dicamba). In general, dicamba permitted formation of significantly more embryoids than 2,4-D. Kinetin (6-furfurylaminopurine) at 2.56 μM or 4.65 μM significantly increased percentage scutellar callus when added to 2,4-D or dicamba-containing medium, respectively. Kinetin at 4.65 μM significantly increased the numbers of embryoids formed when added to medium containing either synthetic auxin. Significantly fewer embryoids formed when cultures were incubated under diffuse light (16-h photoperiod). Casein hydrolysate (200 mg1-1) or L-arginine (0.23 mM) had no effect on numbers of embryoids formed, whereas L-tryptophan (0.20 mM) enhanced such formation with 2,4-D and decreased such formation with dicamba. Two additional experiments generally demonstrated that response to auxin source in the genotypes ND 7532, PCYT 20, Yaqui 50, and Oasis was similar to that in PCYT 10. The higher molar concentration of dicamba required to induce embryogenic callus coupled with more evident embryoid precocious germination and a more rapid rate of tissue necrosis upon extended incubation without subculture suggests that dicamba is metabolized more rapidly than 2,4-D in T. aestivum callus cultures.
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  • 199
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    Nutrient cycling in agroecosystems 12 (1987), S. 157-163 
    ISSN: 1573-0867
    Keywords: barley ; wheat ; nitrate concentration ; plant analysis ; genotypic differences
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Wheat and barley varieties from breeding plots in advanced yield trials were tested for NO3 concentration in their stems at the tillering stage. The study was carried out for three years, at three different locations with a high number of varieties, aiming to determine whether there are varietal differences which could affect the interpretation of prognostic standards set up in a previous study. From nine comparisons between 12 different varieties it was shown that differences in NO3 concentration occurred on only three occasions and these were due to factors which affect NO3 in plants, other than genotypic. The study concluded that varietal differences in NO3 concentration are not a critical consideration for the interpretation of prognostic standards for genotypes which have been developed for certain environmental conditions.
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  • 200
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    Euphytica 36 (1987), S. 521-523 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; plant height ; dwarfing gene ; Rht 12 ; F2 monosomic analysis ; chromosomal location
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The chromosomal location of the dwarfing gene Rht12 in the mutant winter wheat Karcagi 522M7K was investigated using F2 monosomic analysis. The segregation ratio for F2 progenies of Chinese Spring monosomics × Karcagi 522M7K, and that of Cheyenne monosomics × Karcagi 522M7K indicated that the near complete dominant dwarfing gene Rht12 is located on chromosome 5A. The heterozygous and hemizygous states of the genes Rht12 have the same effect on plant height.
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