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  • Chemistry  (47,406)
  • Chemical Engineering  (1,449)
  • AERODYNAMICS
  • Pflanzenkrankheit
  • Wiley-Blackwell  (47,360)
  • Ambleside, UK  (27)
  • FISON  (11)
  • American Association for the Advancement of Science (AAAS)  (9)
  • 2020-2022  (38)
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  • 1
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    Final report on the updating service on environmental chemicals in freshwater for the period 9.1.89 to 9.7.89 (2021)
    Freshwater Biological Association | Ambleside, UK
    In:  http://aquaticcommons.org/id/eprint/5238 | 1256 | 2011-09-29 15:13:42 | 5238 | Freshwater Biological Association
    Details
    Publication Date: 2021-07-09
    Description: This report presents five batches of data which have been despatched to the Joint Research Centre, Ispra. The data as been allocated to various ECDIN (Environmental Chemicals Data and Information Network) files. The data comprises environmental chemicals in the freshwater environment, taking in paricular consideration: aquatic toxicity, bioaccumulation, metabolism and elimination biodegradation.
    Keywords: Chemistry ; Ecology ; Limnology ; Data ; Data processing ; Data collections ; Chemical compounds ; Toxicity ; Bioaccumulation ; Biodegradation ; Metabolism
    Repository Name: AquaDocs
    Type: monograph
    Format: application/pdf
    Format: application/pdf
    Format: 8
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  • 2
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    Changes of water quality and sediment phosphorus of a small productive lake following decreased phosphorus loading (2021)
    Freshwater Biological Association | Ambleside, UK
    In:  http://aquaticcommons.org/id/eprint/5293 | 1256 | 2011-09-29 15:08:09 | 5293 | Freshwater Biological Association
    Details
    Publication Date: 2021-07-09
    Description: Esthwaite Water is the most productive or eutrophic lake in the English Lake District. Since 1945 its water quality has been determined from weekly or biweekly measurements of temperature, oxygen, plant nutrients and phytoplankton abundance. The lake receives phosphorus from its largely lowland-pasture catchment, sewage effluent from the villages of Hawkshead and Near Sawrey, and from a cage-culture fish farm. From 1986 phosphorus has been removed from the sewage effluent of Hawkshead which was considered to contribute between 47% and 67% of the total phosphorus loading to the lake. At the commencement of phosphorus removal regular measurements of phosphorus in the superficial 0-4 cm layer of lake sediment were made from cores collected at random sites. Since 1986 the mean annual concentration of alkali-extractable sediment phosphorus has decreased by 23%. This change is not significant at the 5% level but nearly so. There has been no marked change in water quality over this period. Summer dominance of blue-green algae which arose in the early 1980s after decline of the previous summer forms, Ceratium spp., has been maintained. Improvement in water quality is unlikely to be achieved at the present phosphorus loading.
    Keywords: Chemistry ; Limnology ; Pollution ; Eutrophication ; Freshwater lakes ; Limnological surveys ; Phosphorus ; Phytoplankton ; Primary production ; Sediment analysis ; Water quality ; England ; Esthwaite Water
    Repository Name: AquaDocs
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    Format: 119-131
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  • 3
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    Proximate composition and amino acids profile of smoked catfish Clarias gariepinus and tilapia Oreochromis niloticus (2021)
    FISON | Lagos, Nigeria
    In:  http://aquaticcommons.org/id/eprint/23226 | 19325 | 2018-03-18 14:51:17 | 23226 | Fisheries Society of Nigeria
    Details
    Publication Date: 2021-07-13
    Description: The proximate composition and amino acids assay of smoked Clarias gariepinus and Oreochromis niloticus were determined using standard procedures. The mean proximate composition of Oreochromis niloticus are 8.9% moisture, 16.00% lipids, 5.6% ash, 44.5% crude protein, 0.42% crude fiber and 24.9% NFE while Clarias gariepinus has 8.19% moisture, 15.55% lipids, 4.725 ash, 40.46% crude protein, 0.50% crude fiber and 30.58% NFE. There is therefore a nutrient variation between the 2 species of fish Oreochromis niloticus has higher crude protein, lipids, moisture and ash content than Clarias gariepinus.The amino acids profile shows that both fishes have 17 amino acids. Apart from Tryptophan, the following essential amino acids Histidine, Methionine, Threonine, Isoleucine, Leucine, Lysine, Valine and Phenylalanine are present in both fish. Oreochromis niloticus however has higher quantities of the essential amino acids except for Histidine than Clarias gariepinus
    Description: Includes:- 2 tables.;2 figs.;7 refs.
    Keywords: Chemistry ; Fisheries ; Clarias gariepinus ; Oreochromis niloticus ; Nigeria ; Kainji L. ; freshwater environment ; Amino acids ; Ash content ; Lipids ; Organic compounds ; Moisture ; Proteins
    Repository Name: AquaDocs
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    Format: 91 - 95
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  • 4
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    Evaluations off heavy metals in fish from Lake Geriyo Yola, Nigeria using x-ray fluorescence technique (2021)
    FISON | Akure (Nigeria)
    In:  http://aquaticcommons.org/id/eprint/23338 | 19325 | 2018-04-07 17:17:18 | 23338 | Fisheries Society of Nigeria
    Details
    Publication Date: 2021-07-14
    Description: The evaluation of Cr. Cu, Mn, Fe, Ni, Zn,As and Pb concentrations in Clarias gariepinus and Oreochromis niloticus from Lake Geriyo Yola,Adamawa, Nigeria using X - ray fluorescence (XRF) technique is discussed. The analytical samples are irradiated with high energy electrons of 109Cd to produce fluorescent X-rays was produced which passes to the Silicon-lithium detector through Mo target as a source of monochromatic X-rays. The spectrum of energy generated on the detector was processed by a Multi- Channel Analyzer to obtain analytical data. The intensity of the fluorescent X-rays on the detector is proportional to the concentration of the individual element of interest in the sample. This method can identify up to 30 or more elements at the same time. The results showed varying levels of heavy metals in the fishes. The concentrations of Cr, Mn, Cu, and Ni in the fishes were much higher than WHO and PEPA maximum permissible limits, while the concentrations of Zn and Pb were lower than the standards. The results suggest that the lake is polluted with Cr, Mn, Cu and Ni and the consumption of fishes of the lake is life threatening to man.
    Description: Includes:- 1table.;11 refs.
    Keywords: Ecology ; Chemistry ; Pollution ; Clarias gariepinus ; Oreochromis niloticus ; Nigeria ; Geriyo L. ; freshwater environment ; Heavy metals ; Fish ; X-ray fluorescence analysis ; Pollution ; Pollution effects
    Repository Name: AquaDocs
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    Format: 69-71
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  • 5
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    Effects of salinity on larval dimension of Tilapia guineensis (2021)
    FISON | Lagos (Nigeria)
    In:  http://aquaticcommons.org/id/eprint/23422 | 19325 | 2018-04-25 19:24:46 | 23422 | Fisheries Society of Nigeria
    Details
    Publication Date: 2021-07-14
    Description: Tilapia guineensis eggs were spawned and hatched in different salinities (17, 12, 7, 5, 2 and 0) ppt. The study was conducted in eight replicates. The aim of the investigation was to ascertain disparity in hatching size of Tilapia guineensis attributable to salinity. Immediately hatching was observed, body weight of five hatchlings in each replicate was individually weighed in milligramme. The individual total length of the five hatchlings was also measured in millimeter. The height of the yolk sac larva was determined with micro-metre screw gauge graduated in millimeter from the top of the yolk sac to the dorsal region of the larva. Data obtained were used to calculate yolk sac volume. Significant differences (P〈0.01) were observed in body weight and total length of larvae hatched in different salinities. The heaviest and longest larvae were hatched in 12 ppt saline water. The yolk sac volume of larvae hatched in lower salinities were significantly higher (P〈0.01) than the yolk sac volume of larvae hatched in higher salinities. The smallest yolk sac volume was recorded in 12ppt saline water. Our findings show that salinity could be manipulated for economic production of heavier seeds of Tilapia.
    Description: Includes:- 5 tables.;6 refs.
    Keywords: Aquaculture ; Chemistry ; Tilapia guineensis ; Nigeria ; brackishwater environment ; Salinity effects ; Larvae
    Repository Name: AquaDocs
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    Format: 51-55
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  • 6
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    Physico-chemical parameters of fish ponds as it affects fish production in Ado-Ekiti, Ekiti State, Nigeria (2021)
    FISON | Lagos (Nigeria)
    In:  http://aquaticcommons.org/id/eprint/24590 | 19325 | 2018-05-20 16:05:58 | 24590 | Fisheries Society of Nigeria
    Details
    Publication Date: 2021-07-15
    Description: The water analysis of five selected ponds in Ado-Ekiti in Ekiti State, was carried out to determine their suitability for fish culture. Pa rameters analyzed were: Temperature, Turbidity, Carbon (IV) oxide, Alkalinity, Hardness, pH and Dissolved oxygen. Values for temperature ranged between 20.0~'C and 29.0~'C, Turbidity values were between 46.0-50.2cm, Carbon (IV) oxide: 11.5-12.2mg/litre; alkalinity: 100-120mg/liter; hardness: 110-200mg/litre; pH: 6.8-7.8 and dissolved oxygen: 5.8-6.8mg/liter. The values obtained were found to be within acceptable range for fish production.
    Description: Includes: 10 references.
    Keywords: Aquaculture ; Chemistry ; Nigeria ; Physico-chemical parameter ; Fish pond ; Ado-Ekiti ; Fish production ; freshwater environment ; automation
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  • 7
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    Effect of Terminalia suparba on fecundity and latency period of Clarias gariepinus broodstocks (2021)
    FISON | Minna (Nigeria)
    In:  http://aquaticcommons.org/id/eprint/24067 | 19325 | 2018-05-10 10:15:21 | 24067 | Fisheries Society of Nigeria
    Details
    Publication Date: 2021-07-15
    Description: This study focused on the assessment of Terminalia suparba (bark, leave and root) based diet to improve the ovulation of Clarias gariepinus brood stocks. Ten female and ten male brood stocks were obtained from a reputable fishfarm in Ogun State, Nigeria and were fed with the diets for four weeks before the breeding exercise. The latency period observed in treatments 1 to 4 was 4hours 30minutes while the ovulation period in treatment 5 was 8 hours. There was no significant different (P〉0.05) among the means. The brood stocks in Treatment 2 (Root) has the highest fecundity count (2001) while those in Treatment 5 (control) has the least fecundity count (1496). The phytochemical analysis of the plant materials showed the presence of Alkaloids, Tannin, Phenol, Glycoside, Flavonoids, Steroids, Phlobatannin, Saponin, Anthraquinones. The physical and chemical parameters of water observed showed that dissolved oxygen value ranges from 3.28 -4.60, pH 6.5 - 8.5, temperature 28.0 - 28.8 and conductivity 8.9 - 9.20. It is therefore concluded that the inclusion of Terminalia suparba in the feed of Clarias gariepinus broodstock will not only improve ovulation but also reduce the latency period.
    Description: Includes: 5 tables;Also includes: 11 refs.
    Keywords: Aquaculture ; Chemistry ; Ecology ; Clarias gariepinus ; Nigeria ; broodstocks ; Terminalia specie ; Latency ; fecundity ; Clarias gariepinus ; freshwater environment ; Artificial feeding ; Feeding experiments ; Fecundity ; Brood stocks ; Sublimation heat ; Ovulation ; Physicochemical properties ; Fish culture
    Repository Name: AquaDocs
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  • 8
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    Survey of physical characteristics of the upper reaches of new Calabar River, Niger Delta, Nigeria (2021)
    FISON | Lagos (Nigeria)
    In:  http://aquaticcommons.org/id/eprint/24618 | 19325 | 2018-05-22 06:12:36 | 24618 | Fisheries Society of Nigeria
    Details
    Publication Date: 2021-07-15
    Description: One of the priority research projects of the Nigerian Institute for Oceanography and Marine Research (NIOMR) is broodstock development and mass propagation of silver catfish, Chrysichthys nigrodigitatus of which the broodstock is naturally abundant in New Gala River. Selected physical characteristics and water physico-chemical parameters of the upper reach of the New Calabar River at Aluu were investigated. The highest depth value of 9.20 m was recorded in the middle of the river. The width of the river measured during spring high tide and low tide were 174 m and 110 m respectively. The cross-sectional area and average flow velocity of the river were 797.13 m2 and 0.374 m/s respectively. The discharge of the river was computed as 298.13m3/s. The river water was acidic most of the period (Hydrogen ion concentration, pH: 5.12 to 7.43), with low total alkalinity values (4.00 to 12.00 mgCaCO3/L) and low total hardness values (11.5 to 51.2 mgCaCO3/L) which indicate that the river water is black and soft. There was significant (p~,0.05) seasonal variation in temperature (23.1 to 31.7~'C\}, Secchi transparency (9.30 to 19.00 cm), total hardness (11.5 to 51.2 mgCaCO3/L), dissolved oxygen (5.20 to 7.50 mg/L) and dissolved oxygen deficit (0.02 to 2.95mg/L). These results are useful for several stake holders who use the river as a sink for trade wastes, as source of water for fish farming, as source of broodstocks of Chrysichthys nigrodigitatus, as raw water for water treatment plants, and as means of transportation of industrial materials.
    Description: Includes: 16 references.
    Keywords: Chemistry ; Fisheries ; Limnology ; Chrysichthys nigrodigitatus ; Nigeria ; New Calabar River ; New calabar River ; Physical characteristics and physico-chemical features ; Seasonal variation ; freshwater environment ; automation
    Repository Name: AquaDocs
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    Format: 206-210
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  • 9
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    Physico-chemical parameters in relation to fish production of Dadin Kowa Dam, Gombe State, Nigeria (2021)
    FISON | Lagos (Nigeria)
    In:  http://aquaticcommons.org/id/eprint/24619 | 19325 | 2018-05-22 06:14:16 | 24619 | Fisheries Society of Nigeria
    Details
    Publication Date: 2021-07-15
    Description: Eight month physicochemical study of Dadin Kowa Dam was carried out forthnightly from July, 2011 to february 2012. Air temperature ranged from 18.25~'C ~c 28~'C ~c 0.50 to 28~'C ~c 2.16. Water tmperature ranged from 18.75~'C ~c 2.21. Transparency ranged from 11.00cm ~c 1.83 to 29.63 cm ~c ~c 2.14. Conductivity ranged from 13.00~Ks/cm ~c 13.33 to 41.38~Ks/cm ~c 5.54. Hydrogen ion (pH) ranged from 6.69 ~c 1.03 to 8.39 ~c 1.17. Dissolved oxygen ranged from 6.39mg/l ~c 1.03 to 10.14mg/l ~c 1.17. Total alkalinity ranged from o.69mg/l ~c 0.38 1.34mb/l ~c 0.02. Total ammonia ranged from 0.20mg/l ~c 0.027 to 0.83mg/l ~c 0.05. There were various levels of correlation between these parameters. There was no significant difference between both sites and months (p〉0.05) of all the parameters except total nitrogen which show a significant difference between the sites (p〈0.05) and transparency also shows a significance between the months (p〈0.05). All physicochemical parameters were almost within the tolerable limits for supporting aquatic life.
    Description: Includes: 27 references.
    Keywords: Fisheries ; Chemistry ; Nigeria ; Dadin Kowa Dam ; Physico-chemical parameters ; Fish production ; Dadin Kowa Dam ; freshwater environment ; automation
    Repository Name: AquaDocs
    Type: conference_item , TRUE
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    Format: 211-213
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  • 10
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    Physico-chemical and bacteriological investigation of fish and water in Aiba reservoir, Iwo (2021)
    FISON | Akure (Nigeria)
    In:  http://aquaticcommons.org/id/eprint/23326 | 19325 | 2018-04-06 16:03:20 | 23326 | Fisheries Society of Nigeria
    Details
    Publication Date: 2021-07-14
    Description: This study looked into the physicochemical and bacteriological investigation of fish and water in Aiba Reservoir. Water and fish samples collected were studied to assess their bacteriological and physicochemical characteristics and their suitability for domestic purposes. Structural questionnaires were administered to the fishermen around the reservoir and experiments were carried out in the laboratory to analyze the samples. The results showed that the total viable bacteria count was high(1.02 x 105 cfu/ml) while few Staphylococcus aureus and E. coli occurred insignificantly. The population of S. aureus and E. coli in the fish gills, liver and tissue were insignificant. The results obtained from the physicochemical analysis of the water samples showed that the reservoir has optimum water temperature of 29degreesC ~c 0.25, a pH of 7.70 ~c 0.20, dissolved Oxygen level of 7.20mgO2/1 ~c 0.20 and total hardness of 45.00 mg/ICaC03 ~c 1.05. The microbial load in the fish sample is higher than that in the water sample. Further strict hygienic measures and surveillance is recommended so as to improve the water quality and to avoid deleterious effects on the health of the Iwo inhabitants and its neighboring communities who also benefit from the reservoir.
    Description: Includes:- 2 figs.;11 refs.
    Keywords: Chemistry ; Fisheries ; Nigeria ; Aiba Reservoir ; freshwater environment ; Physicochemical properties ; Bacteriology ; Water quality ; Water ; Bacterial counters
    Repository Name: AquaDocs
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  • 11
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    Primary production and physico-chemical parameters of a freshwater fish farm in the south east Nigeria (2021)
    FISON | Akure (Nigeria)
    In:  http://aquaticcommons.org/id/eprint/23410 | 19325 | 2018-04-12 09:04:25 | 23410 | Fisheries Society of Nigeria
    Details
    Publication Date: 2021-07-14
    Description: Comparative primary production in Little Stream Farms, near Uyo was investigated in relation to physico-chemical parameters of the adjacent perennial stream and the fish ponds. Except for temperature, which showed the same values for both stations, physico-chemical variables generally showed significant difference (P〈O.05) between the pond water and the adjacent stream. Values of silicates, Turbidity (NTU), Secchi Disc Transparency and Total Suspended Solids (TSS) exhibited significantly variable trends (P〈O.05), especially in the stream water, in response to the commercial sand dredging activities upstream from the fann location. Lowest value of stream transparency (O.1M) recorded was during the peak of the sand dredging, whereas the stream water showed absolute transparency in the absence of the dredging. The water quality parameters also exhibited a general trend of significant seasonal variation (P〈O.05). Five phytoplankton families: Bacillariophyceae, Chlorophyceae, Cyanobacteria, Euglenophyceae and Dinophyceae occurred in the ponds, while three families, Bacillariophyceae, Chlorophyceae and Cyanobacteria occurred in the stream. Bacillariophyceae showed the highest relative abundance in both stations A and B.
    Description: Includes:- 1 table.;3 figs.;11 refs.
    Keywords: Aquaculture ; Chemistry ; Ecology ; Nigeria ; Akwa Ibom State ; freshwater environment ; Physicochemical properties ; Freshwater ecology ; Water quality ; Phytoplankton ; Suspended particulate matter
    Repository Name: AquaDocs
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    Format: 170-177
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  • 12
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    Assessment of water quality parameters in Lake Alau (2021)
    FISON | Akure (Nigeria)
    In:  http://aquaticcommons.org/id/eprint/23404 | 19325 | 2018-04-12 08:55:07 | 23404 | Fisheries Society of Nigeria
    Details
    Publication Date: 2021-07-14
    Description: The physico-chemical parameters of Lake Alau were monitored for four months to assess the water quality. Parameters such as temperature, transparency, conductivity, dissolved oxygen, biochemical oxygen demand, Ca, P, etc were studied in five stations (A, B, C, D and E). Water samples were collected monthly from 8:00 to l2:00noon, for four months (July - October 2008, wet season) and analysed. The physico-chemical parameters were within the ranges of unpolluted water bodies. Variation occurs in almost all the months of the study. Significant difference (P〈0.05) occurred in pH, transparency and Ca among the stations, and insignificant difference (P〉0.05) in temperature, conductivity, dissolved oxygen, biochemical oxygen demand, Mg and P. These variations may be due to effects of fertilizer application, herbicides and insecticides in irrigated farms around the Lake.
    Description: Includes:- 2 tables.;24 refs.
    Keywords: Chemistry ; Limnology ; Nigeria ; Alau L. ; freshwater environment ; Physicochemical properties ; Water quality ; Temperature ; Turbidity ; Transparency ; Dissolved oxygen
    Repository Name: AquaDocs
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    Format: 149-152
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  • 13
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    Preliminary studies on some physico-chemical parameters and fish species composition of Doma Dam, Nasarawa State, Nigeria (2021)
    FISON | Lagos (Nigeria)
    In:  http://aquaticcommons.org/id/eprint/24638 | 19325 | 2018-05-24 09:36:38 | 24638 | Fisheries Society of Nigeria
    Details
    Publication Date: 2021-07-15
    Description: Study of some Physico-chemical parameters and fish species composition of Doma Darn was carried out between the month of August 2012. Fishing communities along the dam were selected at sampling stations and collection of fish species. The values of physico-parameters obtained for stations were in the range of 28.10-92 m for depth, 27.70-28.48~'for temperature, 7.03-7.64 for PH, 7.23-7.64mg/l for dissolved oxygen (DO), 30.08-31.75 ntu for turbidity, 94.64-105.17mg/l for alkalinity and 79.17-80.58mg/l for total hardness. The result indicated that there was significant difference (p〈0.5)for depth, temperature turbidity and alkalinity, while there was no significant difference (p〈0.05) for DO, pH and water hardness among the stations. The result of these parameters among the duration shows that there was significant different (p〈0.05) in all the parameters except pH. The study revealed that there were twenty-five (25) fish species belonging to 12 families in the dam. The family Cichlidae and Mochokidae and Bagridae which has three each, While the family, Polypteridae, Channidae, Centropomidae, flepsetidae and Protopteridae has the lowest number of one (1) each. The overall result revealed that the Phvsico-chemical parameters were mostly influenced by duration and not much by stations; and parametersoaram measured were within the recommended water quality requirement for growth and survival of aquatic organisms.
    Description: Includes: 10 references.
    Keywords: Chemistry ; Fisheries ; Nigeria ; Doma Dam ; Doma Dam ; Fishing community ; Physico-chemical parameter ; Fish species ; freshwater environment ; automation
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  • 14
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    The changing chemical composition of chalk streams and rivers (2021)
    Freshwater Biological Association | Ambleside, UK
    In:  http://aquaticcommons.org/id/eprint/5157 | 3949 | 2011-09-29 15:19:58 | 5157 | Freshwater Biological Association
    Details
    Publication Date: 2021-07-08
    Description: The area studied was the River Frome system below Dorchester. The main river has its origins mainly in chalk springs, although some of its tributaries have surface run-off from farm lands and heath-lands. Thus the chemistry of the river is affected by changes in land practice and differences in the geology of the catchment area. Regular chemical analysis of chalk waters started at the River Laboratory in 1964, Regular weekly analyses have been carried out since 1965 at Bere Stream (a small chalk stream) and the River Frome (a large chalk stream); also single samples have been analysed to provide preliminary information. In 1970-71 an attempt was made to discover the contribution each main source made to the flow and chemical composition of the River Frome. Results of these investigations are presented in the paper.
    Keywords: Chemistry ; Environment ; Chemical analysis ; Chemical composition ; Rivers ; Stream flow ; Annual report ; England ; Dorset
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  • 15
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    Geochemistry of lipids in lacustrine sediments (2021)
    Freshwater Biological Association | Ambleside, UK
    In:  http://aquaticcommons.org/id/eprint/5171 | 3949 | 2011-09-29 15:18:48 | 5171 | Freshwater Biological Association
    Details
    Publication Date: 2021-07-08
    Description: An explanation of the basic premises of the subject and its terminology is given. The article then outlines aspects of research on the organic material found in lacustrine sediments, and the analytical methods involved.
    Keywords: Chemistry ; Earth Sciences ; Diagenesis ; Freshwater lakes ; Geochemistry ; Lipids ; Sediment analysis ; Annual report
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  • 16
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    Joint research project on the aquatic ecotoxicity of organo-aluminium: Chemical reactions. First interim report (2021)
    Freshwater Biological Association | Ambleside, UK
    In:  http://aquaticcommons.org/id/eprint/5225 | 1256 | 2011-09-29 15:12:36 | 5225 | Freshwater Biological Association
    Details
    Publication Date: 2021-07-09
    Description: The overall goal of the joint research project is to relate the chemical reactions involved in the formation of organo-aluminium complexes under acid conditions to their toxic effects on the physiology of aquatic organisms. Finally, this research is intended to predict toxic effects arising from acidity and aluminium under varying environmental conditions. This interim report examines the chemical modelling of ion-binding by humic substances where a computer model has been developed and is being tested using field data, and conditions required for the precipitation of aluminium in surface waters.
    Keywords: Chemistry ; Limnology ; Pollution ; Modelling ; Models ; Humic acids ; Surface water ; Aluminium compounds ; Aluminium ; Ions ; Toxicity ; England ; Duddon River
    Repository Name: AquaDocs
    Type: monograph
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  • 17
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    Decomposition in lake sediments: bacterial action and interaction (2021)
    Freshwater Biological Association | Ambleside, UK
    In:  http://aquaticcommons.org/id/eprint/5239 | 3949 | 2011-09-29 15:13:47 | 5239 | Freshwater Biological Association
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    Publication Date: 2021-06-24
    Description: This review discusses the processes involved in the decomposition of organic carbon derived initially from structural components of algae and other primary producers. It describes how groups of bacteria interact in time and space in a eutrophic lake. The relative importance of anaerobic and aerobic processes are discussed. The bulk of decomposition occurs within the sediment. The role of bacteria in the nitrogen cycle and the iron cycle, and in sulphate reduction and methanogenesis as the terminal metabolism of organic carbon are described.
    Keywords: Biology ; Chemistry ; Anaerobic bacteria ; Bacteria ; Biodegradation ; Carbon cycle ; Eutrophic lakes ; Fresh water ; Geochemical cycle ; Iron ; Manganese ; Nitrates ; Nitrogen cycle ; Organic carbon ; Oxygen consumption ; Partical size ; Sedimentation ; Sulphates ; Sulphur ; Annual report
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    Biological and climatic influences on the dace Leuciscus leuciscus in a southern chalk-stream (2021)
    Freshwater Biological Association | Ambleside, UK
    In:  http://aquaticcommons.org/id/eprint/5247 | 3949 | 2011-09-29 15:08:19 | 5247 | Freshwater Biological Association
    Details
    Publication Date: 2021-07-09
    Description: The dace, Leuciscus leuciscus (L.) is an important cyprinid in terms of population biomass in chalk streams of southern England. Dace recruitment has been shown to vary widely from year to year and it is thought that this variation is largely as a result of the influence of abiotic factors, chiefly water temperature. From 1968 to 1981 there was a thirteen-fold difference in the year class structure index between the minimum index (0.25 in 1972) and the maximum (3.21 in 1976). The problems of such variation, especially those that could ensue from a succession of poor year-classes, are offset by the spread of reproductive effort by each female over several years.
    Keywords: Biology ; Chemistry ; Fisheries ; Abiotic factors ; Age composition ; Annual variations ; Climate ; Fecundity ; Fish eggs ; Fish larvae ; Freshwater fish ; Life history ; Mortality ; Population dynamics ; Predation ; Recruitment ; Sexual reproduction ; Survivial ; Water temperature ; Cyprinidae ; Leuciscus leuciscus ; England ; Dorset ; Chalk streams ; Annual report
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  • 19
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    Analysis and adsorption of phosalone on aquatic sediment (2021)
    Freshwater Biological Association | Ambleside, UK
    In:  http://aquaticcommons.org/id/eprint/5257 | 1256 | 2011-09-29 15:09:23 | 5257 | Freshwater Biological Association
    Details
    Publication Date: 2021-07-09
    Description: Phosalone is a non systematic, wide spectrum organophosphate pesticide which was discovered in 1961 in the laboratories of the Societe des Usines Chimique Rhone-Poulenc in France. It has been approved for commercial use since 1964 in France, in Australia since 1966, in the United Kingdom in 1967 and in many other countries including Japan, Egypt, USSR and the USA. This study provides a full literature review on all aspects of phosalone including its physical, biological and chemical characteristics, and analytical methods of analysis with particular reference to soils/sediments. Furthermore, it aims to develop a method for the determintion of phosalone in aquatic sediments and to determine the adsorption of phosalone onto kaolinite.
    Keywords: Chemistry ; Limnology ; Pollution ; Insecticides ; Metabolism ; Toxicity ; Biodegradation ; Adsorption
    Repository Name: AquaDocs
    Type: monograph
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    Format: application/pdf
    Format: 54
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    Petroleum hydrocarbons in fresh waters: a preliminary desk study and bibliography (2021)
    Freshwater Biological Association | Ambleside, UK
    In:  http://aquaticcommons.org/id/eprint/5278 | 1256 | 2011-09-29 15:11:09 | 5278 | Freshwater Biological Association
    Details
    Publication Date: 2021-07-09
    Description: A literature survey was carried out into the effects of petroleum hydrocarbons in freshwater, from the toxicity, biodegradability and concentration aspects. It was supplemented by a selective search on hydrocarbons in the marine environment for comparison. The aim was to determine the major inputs of these hydrocarbons, their accumulation, effects and fate in freshwaters. The search was confined to the period 1965-1978. The bibliography contains 390 references, divided by subject.
    Keywords: Chemistry ; Limnology ; Pollution ; literature reviews ; petroleum ; toxicity ; biodegradation
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    Concentrations of major ions in the stream water of the River Duddon, England, 1970-1974 [Dataset] (2021)
    Freshwater Biological Association | Ambleside, UK
    In:  http://aquaticcommons.org/id/eprint/5349 | 1256 | 2011-05-13 11:55:43 | 5349 | Freshwater Biological Association
    Details
    Publication Date: 2021-07-10
    Description: This dataset provides raw data of chemical analyses made during studies on seasonal variations of some major ions in the stream water of the River Duddon in Cumbria. Measurements of sodium, calcium, potassium, magnesium and chloride ions and pH were taken at 5 stations in the River Duddon between January 1970 and August 1974.
    Description: This data was used in the publication: Carrick, T.R. & Sutcliffe, D.W.: Concentrations of Major Ions in Streams on Catchments of the River Duddon (1971-1974) and Windermere (1975-1978), English Lake District. Freshwater Biological Association, Ambleside, UK, 1983 (FBA Occasional Publications 22)
    Keywords: Chemistry ; Limnology ; Calcium ; chlorides ; fresh water ; ions ; magnesium ; potassium ; seasonal variations ; sodium ; water analysis ; dataset
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    Analysis of stream waters in the catchment of Lake Windermere (1975-1978), England [Dataset] (2021)
    Freshwater Biological Association | Ambleside, UK
    In:  http://aquaticcommons.org/id/eprint/5351 | 1256 | 2011-05-13 11:55:21 | 5351 | Freshwater Biological Association
    Details
    Publication Date: 2021-07-10
    Description: This dataset provides raw data of chemical analyses made during studies on seasonal variations of some major ions in the stream water of the catchment of Lake Windermere in Cumbria. Measurements of sodium, calcium, potassium, magnesium, chloride ions and pH were taken at 37 stations in the catchment between 1975 and 1978.
    Description: This data was used in the publication: Carrick, T.R. & Sutcliffe, D.W.: Concentrations of major ions in streams on catchments of the River Duddon (1971-1974) and Windermere (1975-1978), English Lake District. Freshwater Biological Association, Ambleside, UK, 1983 (FBA Occasional Publications 22)
    Keywords: Chemistry ; Limnology ; Calcium ; chlorides ; fresh water ; ions ; magnesium ; potassium ; seasonal variations ; sodium ; water analysis ; England ; Lake Windermere ; dataset
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    Concentrations of major ions in 182 tarns in the English Lake District (1953-1978) [Dataset] (2021)
    Freshwater Biological Association | Ambleside, UK
    In:  http://aquaticcommons.org/id/eprint/5354 | 1256 | 2011-09-16 12:01:28 | 5354 | Freshwater Biological Association
    Details
    Publication Date: 2021-07-10
    Description: This dataset provides raw data of chemical analyses made during studies on seasonal variations of 182 tarns in the English Lake District, Cumbria. Measurements of sodium, calcium, potassium, magnesium, pH, chloride ions, alkalinity, sulphite, strong acids and nitrate were taken between 1953 and 1978.
    Description: This data was used in the publication: Carrick, T.R. & Sutcliffe, D.W.: Concentrations of major ions in lakes and tarns on the English Lake District (1953-1978). Freshwater Biological Association, Ambleside, UK, 1982 (FBA Occasional Publications 16)
    Keywords: Chemistry ; Limnology ; Calcium ; chlorides ; fresh water ; ions ; magnesium ; potassium ; seasonal variations ; sodium ; water analysis ; alkalinity ; England ; Lake District ; dataset
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  • 24
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    Analyses of treated sewage effluent from Grasmere Treatment Unit (1974-1976), England [Dataset] (2021)
    Freshwater Biological Association | Ambleside, UK
    In:  http://aquaticcommons.org/id/eprint/5352 | 1256 | 2012-07-04 19:32:47 | 5352 | Freshwater Biological Association
    Details
    Publication Date: 2021-07-10
    Description: This dataset provides raw data of chemical analyses made during studies on seasonal variations of treated sewage effluent from Grasmere Treatment Unit in Cumbria. Measurements of sodium, calcium, potassium, magnesium and chloride ions were taken between 1974 and 1976.
    Description: This data was used in the publication: Carrick, T.R. & Sutcliffe, D.W.: Concentrations of major ions in streams on catchments of the River Duddon (1971-1974) and Windermere (1975-1978), English Lake District. Freshwater Biological Association, Ambleside, UK, 1983 (FBA Occasional Publications 22)
    Keywords: Chemistry ; Limnology ; Calcium ; chlorides ; fresh water ; ions ; magnesium ; potassium ; seasonal variations ; sodium ; water analysis ; England ; Grasmere ; dataset
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  • 25
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    Concentrations of major ions in the stream water of the upper basin of the River Duddon, England, 1972-1974 [Dataset] (2021)
    Freshwater Biological Association | Ambleside, UK
    In:  http://aquaticcommons.org/id/eprint/5350 | 1256 | 2011-05-13 11:56:03 | 5350 | Freshwater Biological Association
    Details
    Publication Date: 2021-07-10
    Description: This dataset provides raw data of chemical analyses made during studies on seasonal variations of some major ions in the stream water of the upper basin of the River Duddon in Cumbria. Measurements of sodium, calcium, potassium, magnesium and chloride ions and pH were taken at 26 stations in the River Duddon basin between 1972 and 1974.
    Description: This data was used in the publication: Carrick, T.R. & Sutcliffe, D.W.: Concentrations of Major Ions in Streams on Catchments of the River Duddon (1971-1974) and Windermere (1975-1978), English Lake District. Freshwater Biological Association, Ambleside, UK, 1983 (FBA Occasional Publications 22)
    Keywords: Chemistry ; Limnology ; Calcium ; chlorides ; fresh water ; ions ; magnesium ; potassium ; seasonal variations ; sodium ; water analysis ; England ; River Duddon ; dataset
    Repository Name: AquaDocs
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  • 26
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    Major ions in 25 frequently sampled tarns in the English Lake District (1954-56, 1974-1976) [Dataset] (2021)
    Freshwater Biological Association | Ambleside, UK
    In:  http://aquaticcommons.org/id/eprint/5353 | 1256 | 2012-07-04 19:33:33 | 5353 | Freshwater Biological Association
    Details
    Publication Date: 2021-07-10
    Description: This dataset provides raw data of chemical analyses made during studies on seasonal variations of 25 frequently sampled tarns in Cumbria. Measurements of sodium, calcium, potassium, magnesium, pH, chloride ions, alkalinity, sulphite, strong acids and nitrate were taken between 1954 and 1956 and between 1974-1976.
    Description: This data was used in the publication: Carrick, T.R. & Sutcliffe, D.W.: Concentrations of major ions in streams on catchments of the River Duddon (1971-1974) and Windermere (1975-1978), English Lake District. Freshwater Biological Association, Ambleside, UK, 1983 (FBA Occasional Publications 22)
    Keywords: Chemistry ; Limnology ; Calcium ; chlorides ; fresh water ; ions ; magnesium ; potassium ; seasonal variations ; sodium ; water analysis ; alkalinity ; England ; Lake District ; dataset
    Repository Name: AquaDocs
    Type: other
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  • 27
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    Changes in the phytoplankton (2021)
    Freshwater Biological Association | Ambleside, UK
    In:  http://aquaticcommons.org/id/eprint/5111 | 3949 | 2011-09-29 15:23:22 | 5111 | Freshwater Biological Association
    Details
    Publication Date: 2021-07-08
    Description: An article discussing changes observed in phytoplankton of the Lake District. An overview is given of previous phytoplankton studies undertaken in the area, detailing some changes found in various waterbodies. Water quality changes in Lake Windermere are mentioned, including the gradual increase of dissolved organic matter (DOM), believed to be caused by the increase of sewage to the lake. The lakes in the Alps are given as an example of a similar anthropogenic pollution scenario. The treatment of a Lake District tarn with bone meal is described. The article goes on to discuss the composition of plankton throughout the year under a variety of climatic conditions. A figure shows seasonal variation in the consistuents of phytoplankton in Windermere (north basin).
    Keywords: Biology ; Chemistry ; Limnology ; Phytoplankton ; Limnology ; Dissolved organic matter ; Seasonal variations ; Sewage ; Water quality ; Annual reports ; England ; Lake District
    Repository Name: AquaDocs
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  • 28
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    The chemical budget of a lake (2021)
    Freshwater Biological Association | Ambleside, UK
    In:  http://aquaticcommons.org/id/eprint/5107 | 3949 | 2011-09-29 15:23:50 | 5107 | Freshwater Biological Association
    Details
    Publication Date: 2021-07-08
    Description: A progress report on research undertaken on the chemical budget of a lake, outlining the importance of nitrogen and phosphorus in governing the production of life in freshwater. The report uses the Rivers Brathay and Leven, which flow into Windermere, as examples. The report also refers to the Rivers Rothay, Troutbeck and Cunsey. A table is including which shows the monthly average nitrate content (mg per litre) of the River Brathey and River Leven for 1937 into 1938. The report also includes a figure showing Windermere lake levels, discharge and rainfall during 1937. It also briefly considers possible anthropogenic influences on water quality.
    Keywords: Chemistry ; Ecology ; Limnology ; Phosphorus ; Organic compounds ; Sewage ; Diatoms ; Algae ; Sediment chemistry ; Seasonal variations ; Annual reports ; Nitrates ; River discharge ; England
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    Bacteria in freshwaters (2021)
    Freshwater Biological Association | Ambleside, UK
    In:  http://aquaticcommons.org/id/eprint/5114 | 3949 | 2011-09-29 15:21:32 | 5114 | Freshwater Biological Association
    Details
    Publication Date: 2021-07-08
    Description: An article looking at the numerical distribution of bacteria in lakes, the types most commonly present and the nitrogen-cycle, in Lake Windermere. The methodology is explained, including the sampling frequency and depths, and bacteria culturing techniques. Water bodies other than Lake Windermere were also sampled and these are detailed, including a water reservoir. A summary of the work into horizontal of bacteria is given, including a table showing the number of bacteria per millilitre at different distances from inflow into Windermere. A summary of the work into vertical distribution is also given, including two figures, one showing seasonal variation during 1938-9 in numbers of bacteria at different depths in Winderemere, and the other showing the relationship between the numbers of bacteria at 10 metres depth in Windermere and the rainfull during the previous 7 days. A summary of the types of bacteria found, as well as work on the nitrogen cycle in Esthwaite Water, Lake Windermere and Blelham Tarn are given.
    Keywords: Biology ; Chemistry ; Limnology ; Nitrogen cycle ; Bacteria ; Freshwater ecology ; Limnology ; Seasonal variations ; Water column ; Water reservoirs ; Annual reports ; England ; Lake District
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  • 30
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    Changes in physical and chemical variables (2021)
    Freshwater Biological Association | Ambleside, UK
    In:  http://aquaticcommons.org/id/eprint/5112 | 3949 | 2011-09-29 15:23:20 | 5112 | Freshwater Biological Association
    Details
    Publication Date: 2021-07-08
    Description: An article reviewing the work undertaken looking at the seasonal variation of chemical conditions in water at various depths in lakes. The laboratory tests undertaken for the research is outlined, as well as details of the sampling locations and the staff involved with the work. One figure shows the seasonal variation in the amounts of dissolved substances in the surface water of Windermere during 1936. Another figure shows seasonal varation inthe dry weight of phyto- and zooplankton in Windermere. Seasonal changes are discussed further and a table is included showing chemical conditions in winter and summer for Windermere.
    Keywords: Biology ; Chemistry ; Limnology ; Limnology ; Chemistry ; Seasonal variations ; Diatoms ; Phytoplankton ; Zooplankton ; Water column ; Surface water ; Annual reports ; England ; Lake District
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    Biological surveillance of chalk-streams (2021)
    Freshwater Biological Association | Ambleside, UK
    In:  http://aquaticcommons.org/id/eprint/5122 | 3949 | 2011-09-29 15:22:47 | 5122 | Freshwater Biological Association
    Details
    Publication Date: 2021-07-08
    Description: An article reviewing the methods of biological surveillance of chalk-streams developed and commonly used at that time, with a focus on their application to the River Frome catchment in Dorset. In evaluating the surveillance methods, the author looks at sampling methods (including cores and kick-sampling), the level of identification of macroinvertebrates, and temporal and spatial variations. Responses of indices to organic pollution are also discussed. A number of accompanying figures are also included.
    Keywords: Biology ; Chemistry ; Ecology ; Environment ; Chalk ; Water management ; Cores ; Aquatic communities ; Freshwater ecology ; Invertebrate larvae ; Biological surveys ; Temporal variations ; Spatial variations ; Annual reports ; England
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    The design and development of the Windermere Profiler. An instrument for measuring how various environmental parameters vary with depth in lakes, reservoirs and rivers (2021)
    Freshwater Biological Association | Ambleside, UK
    In:  http://aquaticcommons.org/id/eprint/5123 | 3949 | 2011-09-29 15:22:51 | 5123 | Freshwater Biological Association
    Details
    Publication Date: 2021-07-08
    Description: A review article detailing the background, development and functionality of the Windermere Profiler, a multi parameter environmental monitoring instrument for use in lakes, reservoirs and rivers. The article explains the requirement for regular data collection by the Freshwater Biological Association at Windermere. The article covers the requirements of a profiling instrument, the design considerations, the electronic circuitry, the computer program, the operation of the computer software, the profiler in use and further developments to the design. A number of figures and images accompany the article.
    Keywords: Chemistry ; Environment ; Limnology ; Profilers ; Inland water environment ; Water reservoirs ; Lakes ; Rivers ; Temperature data ; Electrical conductivity ; Dissolved oxygen ; Depth measurement ; Light attenuation ; Annual reports ; England ; Lake District
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    The growth of brown trout (2021)
    Freshwater Biological Association | Ambleside, UK
    In:  http://aquaticcommons.org/id/eprint/5117 | 3949 | 2011-09-29 15:22:21 | 5117 | Freshwater Biological Association
    Details
    Publication Date: 2021-07-08
    Description: An article discussing the factors affecting the rate of growth of brown trout, detailing the research udnertaken at Wray Castle, designed to clarify the influencing factors in different waters and investigate what would be required to increase their size. The article considers factors such as alkalinity, total hardness, the presence of coarse fish in the waterbodies and competition for food. Previous work undertaken on brown trout in Ireland by other authors is reviewed. A figure showing average growth rate of brown trout in five lakes, as determined from their scales (Lough Derg, Windermere, Loch Leven, Ullswater, Haweswater).
    Keywords: Biology ; Chemistry ; Limnology ; Coarse fish ; pH effects ; Water hardness ; Food availability ; Limnology ; England ; Lake District
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    Acid precipitation and its effects on aquatic systems in the English Lake District (2021)
    Freshwater Biological Association | Ambleside, UK
    In:  http://aquaticcommons.org/id/eprint/5189 | 3949 | 2011-09-29 15:16:49 | 5189 | Freshwater Biological Association
    Details
    Publication Date: 2021-07-09
    Description: There is no evidence of an increase in the acidity (lower pH or alkalinity) of water-bodies in the Lake District over the last 50 years. Brown trout occur in acid streams and upland tarns where pH is 4.5-5.2 throughout the year. Their occurrence in such waters in Britain and Ireland has been known for most of this century and there is no previous evidence of harmful effects on salmonid fisheries, though numbers of fish are naturally low. However, many benthic invertebrates that are common in hill-streams where pH is above 5.7 do not occur in more acid streams. This phenomenon occurs in the headwaters of several western rivers in Cumbria. It is not a recent response to "acid rain". Harmful effects of pH are undoubtedly more pronounced in waters that are poor in other dissolved ions. Low concentrations of sodium, potassium, calcium and chloride are especially important and may limit the distributions of some aquatic animals even where pH is above 5.7. The concentration of sulphate ions is usually relatively high but this is not important to the fauna; concentrations are at least two times higher in productive alkaline water-bodies than they are in unproductive acid waters.
    Keywords: Chemistry ; Ecology ; Limnology ; Acid rain ; Acidification ; Alkalinity ; Freshwater pollution ; pH ; Pollution effects ; Invertebrata ; Salmonidae ; England ; Lake District ; Annual report
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  • 35
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    Studies of chemical speciation in naturally anoxic basins (2021)
    Freshwater Biological Association | Ambleside, UK
    In:  http://aquaticcommons.org/id/eprint/5181 | 3949 | 2011-09-29 15:17:17 | 5181 | Freshwater Biological Association
    Details
    Publication Date: 2021-07-09
    Description: The chemical speciation of both metals and non-metals, the use of polarographic techniques, and application to the study of the chemistry of anoxic waters are considered. In the first part of the paper unfamiliar terminology is explained and then an example of simple lake chemistry is presented to illustrate why the concept of speciation is necessary.
    Keywords: Chemistry ; Limnology ; Chemical speciation ; Anoxic basins ; Annual report
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  • 36
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    The kinetics of calcite precipation and related processes (2021)
    Freshwater Biological Association | Ambleside, UK
    In:  http://aquaticcommons.org/id/eprint/5195 | 3949 | 2011-09-29 15:15:06 | 5195 | Freshwater Biological Association
    Details
    Publication Date: 2021-07-09
    Description: This review is concerned with the kinetics of calcium carbonate formation and related processes which are important in many hard waters.
    Keywords: Chemistry ; Calcite ; Calcium carbonates ; Chemical precipitation ; Fresh water ; Geochemical cycle ; Annual report
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  • 37
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    Treading in Mortimer's footsteps: the geochemical cycling of iron and manganese in Esthwaite water (2021)
    Freshwater Biological Association | Ambleside, UK
    In:  http://aquaticcommons.org/id/eprint/5193 | 3949 | 2011-09-29 15:15:03 | 5193 | Freshwater Biological Association
    Details
    Publication Date: 2021-07-09
    Description: A study of the geochemical cycling of iron and manganese in a seasonally stratified lake, Esthwaite water is described. This work is based on speculative ideas on environmental redox chemistry of iron which were proposed by C.H. Mortimer in the 1940's. These observations have been verified and some speculations confirmed, along with a new understanding of the manganese cycle, and detailed information on the particulate forms of both iron and manganese. Details on the mechanisms and transformations of iron have also emerged.
    Keywords: Biology ; Chemistry ; Chemical precipitation ; Freshwater lakes ; Iron ; Manganese ; Oxidation ; Redox potential ; Reduction ; Stratification ; England ; Esthwaite Lake ; Annual report
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  • 38
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    Long-term natural acidification of upland sites in Cumbria: Evidence from post-glacial lake sediments (2021)
    Freshwater Biological Association | Ambleside, UK
    In:  http://aquaticcommons.org/id/eprint/5197 | 3949 | 2011-09-29 15:15:33 | 5197 | Freshwater Biological Association
    Details
    Publication Date: 2021-07-09
    Description: Studies by the Freshwater Biological Association over the last 25 years have supplied data relevant to the levels of acidity in local soils and water before the onset of industrial pollution and current interest in acid rain. This article reviews published analysis from cores of lake sediments, in or near the catchment of the River Duddon. Electron spin resonance spectra of humic acids and iodine values confirm evidence from pollen analysis for a history of progressive acidification of the source material of lake sediments since before 5000 radiocarbon years, in upland catchments of the Lake District. Processes involved included: removal of basic ions from soils by rainfall, the effects of which were intensified by removal by man of deciduous forest; acidification of soils and waters by decomposition products of Calluna and further acidification of waters by Sphagnum species which colonized habitats where drainage became impeded by paludification processes.
    Keywords: Biology ; Chemistry ; Earth Sciences ; Acid rain ; Acidification ; Freshwater lakes ; Lake Basins ; Lake deposits ; pH ; Palnology ; England ; Cumbria ; Annual report
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    20 years after the wall. Aufbau Ost: Max Planck's East German experiment (2009)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2009-11-07
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Vogel, Gretchen -- New York, N.Y. -- Science. 2009 Nov 6;326(5954):788-91. doi: 10.1126/science.326_788.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/19892956" target="_blank"〉PubMed〈/a〉
    Keywords: *Academies and Institutes/economics/organization & administration ; Anthropology ; Biology ; Chemistry ; Germany ; Germany, East ; Physics ; Research Personnel ; Universities
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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    Engineering entropy-driven reactions and networks catalyzed by DNA (2007)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2007-11-17
    Description: Artificial biochemical circuits are likely to play as large a role in biological engineering as electrical circuits have played in the engineering of electromechanical devices. Toward that end, nucleic acids provide a designable substrate for the regulation of biochemical reactions. However, it has been difficult to incorporate signal amplification components. We introduce a design strategy that allows a specified input oligonucleotide to catalyze the release of a specified output oligonucleotide, which in turn can serve as a catalyst for other reactions. This reaction, which is driven forward by the configurational entropy of the released molecule, provides an amplifying circuit element that is simple, fast, modular, composable, and robust. We have constructed and characterized several circuits that amplify nucleic acid signals, including a feedforward cascade with quadratic kinetics and a positive feedback circuit with exponential growth kinetics.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Zhang, David Yu -- Turberfield, Andrew J -- Yurke, Bernard -- Winfree, Erik -- New York, N.Y. -- Science. 2007 Nov 16;318(5853):1121-5.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Computation and Neural Systems, California Institute of Technology, MC 136-93, 1200 East California Boulevard, Pasadena, CA91125, USA. dzhang@dna.caltech.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/18006742" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Catalysis ; Chemical Engineering ; *Computers, Molecular ; DNA/*chemistry ; Entropy ; Equipment Design ; Feedback, Physiological ; Mice ; Nanotechnology ; Nucleic Acid Hybridization ; Rabbits
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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    Don't forget long-term fundamental research in energy (2007)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2007-02-10
    Description: Achieving a fundamental understanding of the phenomena that will underpin both global stewardship and future technologies in energy calls for a thoughtful balance between large-scale immediate solutions using existing technology and the fundamental research needed to provide better solutions in the 50-year period.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Whitesides, George M -- Crabtree, George W -- New York, N.Y. -- Science. 2007 Feb 9;315(5813):796-8.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA 02138, USA. gwhitesides@gmwgroup.harvard.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17289985" target="_blank"〉PubMed〈/a〉
    Keywords: Biomass ; Biotechnology ; Carbon Dioxide/chemistry ; Catalysis ; Chemical Phenomena ; Chemistry ; Electricity ; Electrodes ; *Energy-Generating Resources ; Environment ; Photosynthesis ; *Research ; Solar Energy
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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    Breakthrough of the year. The runners-up (2007)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2007-12-22
    Description: The runners-up for 2007's Breakthrough of the Year include advances in cellular and structural biology, astrophysics, physics, immunology, synthetic chemistry, neuroscience, and computer science.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉New York, N.Y. -- Science. 2007 Dec 21;318(5858):1844-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/18096772" target="_blank"〉PubMed〈/a〉
    Keywords: Algorithms ; Animals ; Cellular Reprogramming ; Chemical Phenomena ; Chemistry ; Cosmic Radiation ; Humans ; Imagination ; Memory ; Physical Phenomena ; Physics ; Pluripotent Stem Cells ; Receptors, Adrenergic, beta-2/chemistry ; *Science ; T-Lymphocyte Subsets/cytology/immunology
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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    Evolution. Darwin for all seasons (2006)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2006-07-22
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Szathmary, Eors -- New York, N.Y. -- Science. 2006 Jul 21;313(5785):306-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Institute of Biology, Eotvos University Budapest, and Collegium Budapest (Institute for Advanced Study), 2 Szentharomsag utca, H-1014 Budapest, Hungary. szathmary@colbud.hu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/16857926" target="_blank"〉PubMed〈/a〉
    Keywords: *Biological Evolution ; Chemical Phenomena ; Chemistry ; Computational Biology ; Cooperative Behavior ; Cultural Evolution ; Exobiology ; Humans ; Language ; Models, Biological ; Models, Theoretical ; Molecular Biology ; Origin of Life ; *Research ; Selection, Genetic
    Print ISSN: 0036-8075
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    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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    Herve This profile. The joy of evidence-based cooking (2006)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2006-11-25
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Enserink, Martin -- New York, N.Y. -- Science. 2006 Nov 24;314(5803):1235-6.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/17124302" target="_blank"〉PubMed〈/a〉
    Keywords: Chemistry ; *Cooking ; *Food ; France ; History, 20th Century ; History, 21st Century
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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    France. Chemist claims innocence to spying charge (2006)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2006-04-29
    Description: 〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Enserink, Martin -- New York, N.Y. -- Science. 2006 Apr 28;312(5773):512.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/16645058" target="_blank"〉PubMed〈/a〉
    Keywords: Chemistry ; China ; *Fatty Acids ; France ; History, 21st Century ; *Theft
    Print ISSN: 0036-8075
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    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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    Cyberinfrastructure for e-Science (2005)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2005-05-10
    Description: Here we describe the requirements of an e-Infrastructure to enable faster, better, and different scientific research capabilities. We use two application exemplars taken from the United Kingdom's e-Science Programme to illustrate these requirements and make the case for a service-oriented infrastructure. We provide a brief overview of the UK "plug-and-play composable services" vision and the role of semantics in such an e-Infrastructure.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Hey, Tony -- Trefethen, Anne E -- New York, N.Y. -- Science. 2005 May 6;308(5723):817-21.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Engineering and Physical Sciences Research Council, Polaris House, North Star Avenue, Swindon SN2 1ET, UK.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/15879209" target="_blank"〉PubMed〈/a〉
    Keywords: Chemical Phenomena ; Chemistry ; Combinatorial Chemistry Techniques ; *Computational Biology ; *Computer Communication Networks ; *Computing Methodologies ; Databases as Topic ; Graves Disease/genetics ; *Internet ; *Research ; *Software ; Williams Syndrome/genetics
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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    The class of 2005. United States: two scientists and a baby (2005)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 2005-10-22
    Description: If you trust the conventional wisdom, Amy Palmer and Alexis Templeton did a lot of things wrong in their job search. Then why did things turn out so right?〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Austin, Jim -- New York, N.Y. -- Science. 2005 Oct 21;310(5747):518-9.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/16239480" target="_blank"〉PubMed〈/a〉
    Keywords: *Career Choice ; *Career Mobility ; Chemical Phenomena ; Chemistry ; Education, Graduate ; *Faculty ; Microbiology ; *Research ; United States ; *Universities
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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    Electronic Resource
    Electronic Resource
    Masthead (1969)
    New York : Wiley-Blackwell
    Biopolymers 8 (1969) 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.1969.360080101
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    Electronic Resource
    Electronic Resource
    Influence of temperature on the helix sense of several polyamino acids (1969)
    New York : Wiley-Blackwell
    Biopolymers 8 (1969), S. 21-27 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: An increase in temperature causes an increase in the amplitudes of intramolecular and intermolecular motions and mainfests itself as an increase in the effective radii of the various atoms, especially hydrogen atoms. If one uses a Lennard-Jones 6-12 potential function to account for nonbonded interactions, this increase in the radius of the hydrogen atom is equivalent to a modification of the coefficient of the repulsive part of the Lennard-Jones potential. Accordingly, this effect of increasing temperature on the helix sense of several polyamino acids is computed by allowing the effective radius of the hydrogen atom to increase. It is found that the preferred sense of several polyamino acid helices changes from right- to left-handed as the temperature is increased. This accounts for recent experimental observations of changes in helix sense with temperature.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.1969.360080103
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  • 50
    Electronic Resource
    Electronic Resource
    Helix-coil transition for poly(L-glutamic acid) in water-ethanol solutions (1969)
    New York : Wiley-Blackwell
    Biopolymers 8 (1969), S. 57-68 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Potentiometric titrations and some complementary optical rotation data are presented for solutions of poly(L- glutamic acid) (PGA) in several H2O-ethanol mixtures. The data allow the determination of the intrinsic pK (pK0), slope of the apparent. pK (pKapp), versus degree of ionization curves and of the enthalpy of ionization as a function of ethanol concentration. The variation of the degree of ionization at which the helix-coil transformation occurs with ethanol and temperature is also determined. Finally free energy, enthalpy, and intropy changes associated with the helix-coil transformation for the uncharged conformers are determined from the titration curves. The effect of the ethanol is to increase the stability of the helical conformation of PGA for both the charged and the uncharged forms of the polymer. The stabilization of the uncharged helix is essentially an entropic effect.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.1969.360080106
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    Electronic Resource
    Electronic Resource
    Low-frequency infrared bands and chain conformations of polypeptides (1969)
    New York : Wiley-Blackwell
    Biopolymers 8 (1969), S. 91-99 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Infrared spectra of polypeptides were measured in the region of 1800-400 cm-1. For the α-helical form, disordered form, and antiparallel-chain β-form, amide V band- arising from N-H out-of-plane bending models were observed at 610-620, around 650, and 700-705 cm-1, respectively, and amide V′ bands arising from N-D out-of-plane bending modes were observed at 455-465, around 510, and a 515-530 cm-1, respectively. These correlations are useful for conformation diagnoses, particularly for copolyamino-acids or proteins which are not oriented. The nature of low-frequency amide bands are discussed with reference to potential energy distributions calculated for the α-helical form and β form.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.1969.360080108
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    Electronic Resource
    Electronic Resource
    Etude, par spectroscopie infra-rouge, de la conformation de quelques composés peptidiques modèles (1969)
    New York : Wiley-Blackwell
    Biopolymers 8 (1969), S. 69-89 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Some experimental data are given on the infrared spectra between 3300 and 3500 cm-1 of dilute solutions in carbon tetrachloride of three types of model compounds: CH3-CONH-CH(R1)-CONH(R2), (I); CH3-CON(CH3)-CH(R1)-CONH(R2), (II) and CH3-CONH-CH(R1)-CON(R2)2, (III). In studying the N-H stretching bands, it was found that there are two types of intramolecular hydrogen bonds in these molecules; these result in two different cyclized conformations, C5 and C7, which contain respectively, five and seven atoms in the ring. By using model substances I, II, and III, in which the nitrogen atoms are unequally substituted, it is possible to identify the N-H stretching bands which are to be ascribed to the N-H oscillators included in the two different chelated conformations. It is found also that the stretching frequency of a free N-H oscillator depends upon the substituent on the nitrogen atom. Thus, it is possible to observe, with some of the model compounds I, four different absorption bands located at 3340, 3420, 3440, and 3460 cm-1. The first two are ascribed to the N-H oscillators included in the H—bonds which lock the C7 and C5 conformations; the last two correspond to free N-H which differ with the substituent on the nitrogen atom.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.1969.360080107
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    Electronic Resource
    Electronic Resource
    Interaction of poly-L-lysine with nucleic acids. V. Effect of salt concentration (1969)
    New York : Wiley-Blackwell
    Biopolymers 8 (1969), S. 153-155 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.1969.360080112
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  • 54
    Electronic Resource
    Electronic Resource
    Quantum mechanical approach to the conformational analysis of macromolecules in ground and excited states (1969)
    New York : Wiley-Blackwell
    Biopolymers 7 (1969), S. 207-213 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Molecular orbital calculations of the extended Hückel type have been used to study the conformations of glycyl and alanyl residues in ground and excited states. The ground-state surfaces show features similar to those obtained with the standard calculational methods in which the total energy is partitioned into components such as torsions, nonbonded and electrostatic interactions. The molecular orbital calculations provide the first independent theoretical check on such calculations. The excited-state surfaces, Uniquely available from the molecular orbital calculations, exhibit a better definition and sharpening of potential minima in the sterically allowed regions.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.1969.360070207
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    Electronic Resource
    Electronic Resource
    Computation of molecular weight averages for DNA molecules containing both preformed and randomly induced single-strand breaks (1969)
    New York : Wiley-Blackwell
    Biopolymers 7 (1969), S. 215-221 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: We derive equations for calculating the number-, weight-, and z-average lengths between single-strand breaks in DNA molecules. Provision is made for the existence of preformed breaks under genetic control: these are assumed to be fixed in number in each molecule, but may be variable in position. Breaks due to random degradation may be superimposed on the pre-existing breaks. An example is given for illustrative purposes.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.1969.360070208
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    Electronic Resource
    Electronic Resource
    Studies on the secondary structure of ribosomal ribonucleic acid components of rabbit reticulocytes (1969)
    New York : Wiley-Blackwell
    Biopolymers 7 (1969), S. 223-239 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The conformation in solution of fractionated 30 S and 19 S ribosomal RNA from rabbit reticulocytes has been studied by optical rotatory dispersion, analysis of thermal melting profiles and their derivatives, and spectrophotometric acid-base titration. From a consideration of the limitations of these methods, it has been possible to set limiting values on the degree of base-pairing and the lengths of the double helices: between 60 and 80% of the bases in 19 S and 30 S RNA are estimated to be paired. The paired segments are not shorter than 4 base pairs, and evidence from other sources is available which indicates that they are not longer than 8-16 base pairs. The spread of helix lengths is greater in the 30 S than in 19 S RNA; and other differences are noted. Several distinct populations of double helices, differing in their thermal stability, are present. Estimates are presented from spectrophotometric and titration data for the base compositions of the paired and unpaired regions.
    Additional Material: 9 Ill.
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    URL: http://dx.doi.org/10.1002/bip.1969.360070209
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  • 57
    Electronic Resource
    Electronic Resource
    Instability in diffusing fluid layers (1969)
    New York : Wiley-Blackwell
    Biopolymers 7 (1969), S. 251-263 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A small perturbation analysis is carried out to determine the stability of a fluid containing two layers of diffusing solutes in a common solvent and acted upon by a uniform gravitational field. It is found that instability can arise even though the unperturbed diffusion does not lead to the formation of a density inversion within the fluid.
    Additional Material: 5 Ill.
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    URL: http://dx.doi.org/10.1002/bip.1969.360070211
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    Electronic Resource
    Abbreviated nomenclature of synthetic polypeptides (polymerized amino acids). Tentative rulesDocument of the IUPAC-IUB Commission on Biochemical Nomenclature (CBN), approved by IUPAC and IUB in October, 1967, and published by permission of the International Union of Pure and Applied Chemistry, the International Union of Biochemistry, and the official publishers of IUPAC, Messrs. Butterworths Scientific Publications. (1969)
    New York : Wiley-Blackwell
    Biopolymers 8 (1969), S. 161-166 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.1969.360080202
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  • 59
    Electronic Resource
    Electronic Resource
    Kinetics of denaturation of DNA (1969)
    New York : Wiley-Blackwell
    Biopolymers 7 (1969), S. 475-493 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The kinetics of denaturation of DNA have been studied by relaxation techniques. Examination of the terminal relaxation times for a variety of DNA's under a variety of conditions has shown that DNA denaturation is principally a hydrodynamically limited process. Measurements within the helix-coil transition have demonstrated that the experimentally measured terminal relaxation times are a function of the following: (1) position in the helix-coil transition; (2) ionic strength of the solvent; (3) solvent viscosity; (4) DNA concentration; (5) molecular weight; (6) number and position of single-strand breaks. The dependence of the terminal relaxation time on the above mentioned factors can be attributed to hydrodynamic effects. Thus a hydrodynamic model for DNA unwinding is required. The model which best fits the data involves the assumption of a rotational frictional coefficient independent of molecular weight. This assumption is suggested by the fact that the relaxation time is proportional to the first power of the molecular weight.
    Additional Material: 9 Ill.
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    URL: http://dx.doi.org/10.1002/bip.1969.360070406
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    Electronic Resource
    Electronic Resource
    Counterion binding in partially neutralized poly(D-glutamic acid) and poly(DL-glutamic acid) (1969)
    New York : Wiley-Blackwell
    Biopolymers 7 (1969), S. 495-502 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Sodium counterion association with partially neutralized poly(D-glutamic acid) or poly(DL-glutamic acid) was measured by use of Wall's transference method with radioactive sodium. In the region where both polyacids are in completely random coil form, fractions of association were considerably less than that with poly(acrylic acid) in the same region of degree of neutralization. Even in the region where poly (D-glutamic acid) is in the helical form, the fraction of association was less than that with poly(acrylic acid) in the same region. No pronounced characteristics attributable to counterion association corresponding to the helix-coil transition could be found. The association phenomena were discussed on the basis of a rodlike model of polyelectrolyte.
    Additional Material: 2 Ill.
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    URL: http://dx.doi.org/10.1002/bip.1969.360070407
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    Electronic Resource
    Electronic Resource
    NMR studies in solution of poly-L-proline (1969)
    New York : Wiley-Blackwell
    Biopolymers 7 (1969), S. 411-415 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The isomerization of poly-L-proline in different solvents has been studied by NMR spectroscopy. Different resonance signals for the CHα protons have been obtained for the two different helical conformations of thus compound, namely form I and form II.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.1969.360070311
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    Synthesis of poly-L-asparagine and poly-L-glutamine (1969)
    New York : Wiley-Blackwell
    Biopolymers 7 (1969), S. 417-422 
    Details
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    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: Poly-β-N-diphenylmethyl-L-asparagine and poly-γ-N-diphenylmethyl-L-glutamine were prepared from the corresponding N-carboxyanhydrides. Poly-L-aspuragine and poly-L-glutamine were obtained by removal of the diphenylmethyl protecting groups with liquid anhydrous hydrofluoric acid.
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    Hydrogen-tritium exchange of the random chain polypeptide (1969)
    New York : Wiley-Blackwell
    Biopolymers 7 (1969), S. 379-393 
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    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: The Hydrogen-tritium exchange character of poly-D,L-alanine was studied in detail as a model for the hydrogen exchange behavior of the unhindered, polymeric peptide group. The random chain nature of poly-D,L-alanine was evident in the uniformity of exchange rate of all its hydrogens and in the similarity between this rate and that of random chain poly-D,L-lysine and other known, unhindered secondary amide groups. An equilibrium isotope effect favoring the binding of tritium over protium to the extent of 21% was measured. Specific acid and base catalysis of the exchange and the absence of detectable general catalysis were demonstrated. Apparent energy of activation is 17 kcal/mole for deprotonation, largely due to dependence of Kw on temperature, and 15 kcal/mole for protonation, which correlates with the extreme apparent pK. The hydrogen -tritium exchange half-time rate; of poly-D,L-alamine at any pH and temperature (T: °C) is given by the equation: \documentclass{article}\pagestyle{empty}\begin{document}$$ \[t_{\frac{1}{2}} \,(\min )\, = \,200\,\, \times \,10^{0.05{\rm T}} /\,[10^{{\rm pH} - 3} \, + \,10^{3 - {\rm pH}} ]\] $$\end{document}
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    Optical rotation of some α-1,4-linked glucopyranosides in the system H2O-DMSO and solution conformation of amylose (1969)
    New York : Wiley-Blackwell
    Biopolymers 7 (1969), S. 581-593 
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    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The specific rotation of starch components, corrected for refractive index variation, exhibits a discontinuity in the region of the water-dimethyl sulfoxide (H2O-DMSO) system that corresponds to the composition of the complex 2H2O-DMSO. This discontinuity is a property dependent upon the presence of a number of consecutively linked α-1,4 glucose units and, therefore, must reflect a change in symmetry of a segment, of polymer chain. The optical rotation of amylose between 26.5 and 92.5°C. does not change in DMSO and is only slightly lowered in water at the higher temperature. The behavior of amylose in both DMSO and H2O is like that of a random coil, as indicated by viscosity and sedimentation measurements. These results may be interpreted either as being compatible with models of amylose in solution in which the polymer backbone has helical twist, or as indicating removal of strong interactions between polymer chain segments by a good solvent.
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    Calorimetric investigation of the influence of polymer concentration on the transition enthalpy in the helix-coil transition of the double-stranded helical complex poly(A + U) (1969)
    New York : Wiley-Blackwell
    Biopolymers 7 (1969), S. 611-613 
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    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 3 Ill.
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  • 66
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    Polarized ultraviolet absorption spectra and electronic transitions of poly-L-proline (1969)
    New York : Wiley-Blackwell
    Biopolymers 7 (1969), S. 614-618 
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    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
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    Masthead (1969)
    New York : Wiley-Blackwell
    Biopolymers 7 (1969) 
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    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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    John Warren Williams (1969)
    New York : Wiley-Blackwell
    Biopolymers 7 (1969), S. 1-3 
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    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Chemically reacting systems of the type A + B ⇄ AB. II. Osmometry (1969)
    New York : Wiley-Blackwell
    Biopolymers 7 (1969), S. 5-19 
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    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The osmotic pressure equation for nonideal, associating systems of the type nA +mB ⇄ AnBm, has been derived, by using the assumption yAnBm/yAnyBm = 1. This treatment can also be applied to related associations such as nA + mB ⇄ AB + AB2 + A2B + …. From osmotic pressure experiments on the pure reactants it is possible to obtain the molecular weights (MA and MB) of the reactants and also the virial coefficients (BAA and BBB) of the reactants. The osmotic pressure of a nonreacting mixture of A and B can be calculated from these measurements. It can be used along with osmotic pressure measurements on equilibrium mixtures of A and B to obtain expressions containing the equilibrium constant (or constants) and the cross-virial coefficients (BAB and BBA). Several procedures are described for the evaluation of the equilibrium constant (or constants) and the BAB or BBA terms. It appears that this procedure is a general one which is applicable to associations of the type nA + mB ⇄ AB + A2B + AB2 + …. By correcting for nonideal behavior, one should then be able to apply it to any method available for analyzing ideal associations of the types considered here. In addition it is possible, subject to certain restrictions, to analyze associations of the type 3A + B ⇄ A2 + AB.
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    Differential elution of histones from gel-trapped nuclei (1969)
    New York : Wiley-Blackwell
    Biopolymers 7 (1969), S. 39-58 
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    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A method has been developed for characterizing at, submicrogram levels the heterogeneity of histones from purified nuclei. The histones are eluted with a smooth concentration gradient from nuclei trapped in polyacrylamide-gel threads and are collected in a micro fraction collector suitable for volumes in the 10-100 μl range. The gradient and fraction collection systems are governed by cam driven syringes. Samples obtained are subjected to electrophoresis in a starch-gel system and the gels are stained with a highly sensitive stain specific for guanidinium groups. Seven major and a similar number of minor components are demonstrated in the histones. The method of differential elution of trapped macromolecules is suitable for use with systems other than nuclei and histones.
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    Evaluation of lipoprotein size-density distributions from sedimentation coefficient distributions obtained at several solvent densities. I. Theory (1969)
    New York : Wiley-Blackwell
    Biopolymers 7 (1969), S. 119-132 
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    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A method is presented for the treatment of distribution functions obtained by ultra-centrifugal sedimentation velocity experiments on the plasma lipoproteins, so that adequate corrections can he made for the effects of diffusion and concentration dependence of the sedimentation coefficient. The method involves the use of modified equations derived from those presented in 1952 by Gosting for the transform from g* (S) to g(S) distributions. The evaluation of the molecular parameters of lipoprotein density and size, from a set of g(Si°) distribution functions obtained in solvents of varying density, is also outlined. Preparations of the β-lipoprotein of human plasma are found to follow a bivariate normal distribution that requires evaluation of five parameters, x̄1, σ1, x̄2, σ2and p. The first two represent the mean and standard deviation of the lipoprotein density, the third and fourth the same quantities for the size, expressed as ft “logarithmic diameter parameter”, and the final value is a measure of the correlation between these two variables.
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    Conformations of poly-L-valine in solution (1969)
    New York : Wiley-Blackwell
    Biopolymers 7 (1969), S. 133-133 
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    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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    Masthead (1969)
    New York : Wiley-Blackwell
    Biopolymers 7 (1969) 
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    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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    URL: http://dx.doi.org/10.1002/bip.1969.360070201
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    Nuclear magnetic resonance investigation of the helix to random coil transformation in poly-α-amino acids. I. Poly-L-alanine (1969)
    New York : Wiley-Blackwell
    Biopolymers 7 (1969), S. 155-171 
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    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: High-resolution nuclear magnetic resonance spectra at 100 MHz and 220 MHz have been obtained on two samples of poly-L-alanine of differing molecular weights (2500 and 42 500) in the chloroform-trifluoroacetic acid system under various conditions of solvent composition, temperature, and polypeptide concentration. Separate helix and random coil peaks are observed for the α-CH and peptide NH backbone proton resonances, thereby permitting the determination of helix content. This observation of separate peaks demonstrates that the lifetimes of the helix and random coil portions of poly-L-alanine have lower limits of about 10-1 sec. It is suggested that solvent-peptide versus peptide-peptide hydrogen bond competition, coupled with a destabilizing effect of the trifluoroacetic acid on the helix, is responsible for the helix-random coil transformation.
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    Preparative fractionation of lignin by gel-permeation chromatography (1969)
    New York : Wiley-Blackwell
    Biopolymers 7 (1969), S. 135-153 
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    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The combination of an agarose gel (Bio-Gel A) and a dioxane-water (1:1) solvent system allowed the fractionation, on a preparative scale, of a very polydisperse, non-derivatized lignin preparation (enzymatically liberated lignin prepared from sweetgum sapwood with Lenzites trabea). Three fractions differing markedly in molecular weight were obtained. A gel of crosslinked alkylated dextran (Sephadex LH-20) with the same solvent system allowed division of the lowest molecular weight fraction into two fractions. These materials were characterized by measurements of intrinsic viscosity and number-average molecular weights in dimethylformamide and dioxane-water. It was established that the two highest molecular weight fractions were associated in an average trimeric form in dioxane-water (1:1) as compared to the form (considered to be molecular) that occurred in dimethylformamide. Molecular size distributions and eluant volumes of the fractions were determined with a Sephadex G-100-formamide system, the latter being one of the most powerful nonaqueous solvents for lignin. Adsorption effects were known to be absent in this case, and the lignin molecules were considered to be unassociated in formamide. The four fractions were distinguishable with the formamide-G-100 system, thus indicating that the original fractionation was based on molecular size. The enzymatically liberated lignin contained molecules that comprised a continuum of molecular weights from approximately monomeric to molecules that were at the limit of the solvating power of dioxane-water (1:1) and dimethylformamide. Limited physicochemical data were consistent with a compact, approximately spherically symmetric shape of the lignin in solution.
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    Intrachain potential energy of the α-helix and of a coiled coil strand (1969)
    New York : Wiley-Blackwell
    Biopolymers 7 (1969), S. 189-197 
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    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A comparison has been made of the intrachain potential energy of an infinite straight α-helix of poly-L-alanine with that of the distorted form adopted in a coiled coil conformation. The energy terms included were the van der Waal's, electrostatic, hydrogen-bond, and the rotational potential terms. The results indicate that the potential energies of the structures investigated are almost the same, and so a transformation from one state to another may occur without significant changes in potential energy. Particular care has been taken to ensure that the electrostatic and van der Waal's interaction terms are fully convergent. The values obtained for the α-helix were compared with those already published, and some significant differences were found.
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    Optical rotatory dispersion of mucopolysaccharides and mucopolysaccharide-dye complexes. II. Ultraviolet cotton effects in the amide transition bands (1969)
    New York : Wiley-Blackwell
    Biopolymers 7 (1969), S. 173-187 
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    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Ultraviolet optical rotatory dispersion curves of mucopolysaccharides exhibit particular Cotton effects in the spectral region of the n-π and π-π amide transitions. Two general patterns emerge: (1) enhancement of negative rotation and of the first negative Cotton effect (troughs 217-220 mμ) and (2) relative dominance of the positive Cotton effect in the π-π transition region (peak ∼198 mμ). Groups (1) and (2) can be correlated with a structural difference in the linkages of the amino sugars: (1) occurs with polymers containing 3-1-linked glycosamino sugars and (2) with glycosamino moieties linked 4-1 by either α- or β-glycosidie bonds. Measurements of the circular dichroic absorption bands support the qualitative conclusions from optical rotation. All mucopolysaccharides exhibit a first, negative band centered at 208-211 mμ, while only those in group (2) show, in addition, a positive band centered at 189-192 mμ. A suggested unifying model considers that difference in kind and/or degree of preferred geometry of the amide groups obtains from two forms of secondary order: (1) having a linear hydrogen bond from the N (acceptor) to the (C2)O - H of the preceding uronic acid and (2) having a linear hydrogen bond from the N (acceptor) to the (C2 or 3) O - H of the following sugar. The hydrogen bonds would have similar strength but opposite directions in two systems towards the nonreducing end (1) or towards the reducing end (2)], closing eight-membered rings
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    Studies on the myosin molecule from smooth muscle (1969)
    New York : Wiley-Blackwell
    Biopolymers 7 (1969), S. 99-106 
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    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The myosin molecule was extracted from the smooth muscle parts of horse esophagus and purified by ammonium sulfate fractionation. The schlieren pattern of the sedimentation velocity run showed a very sharp single peak of.5.9. S (s20,w). Molecular weight of the protein was measured by means of the Archibald and sedimentation equilibrium methods, both in 0.5M KCI buffered by 1/150 M phosphate at pH 7.5 and at 5°C. The values obtained were 6.25 × 105 and 5.81 × 105respectively, for the two methods. The second virial coefficients were 1.1 × 104 and 1.2 × 10-4 ml/g. Denatured smooth muscle myosin was prepared in a solution of 5M guanidine HC1 containing 0.4 M KC1 and 0.2 M β-mercaptoet hanol buffered at pH 8.0. The weight-average molecular weight of the denatured smooth muscle myosin was 2.24 × 105 and the second virial coefficient was 7.6 × 10-4 ml/g. The values described above are in good agreement with those reported for rabbit skeletal myosin with ammonium sulfate fractionation. The molecular dimension of the molecule is estimated as the value for an axial ratio of 100, assuming a rigid rod molecular model for this molecule, both the thermodynamical and hydrodynamical treatment being in a good agreement with this estimation.
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    Masthead (1969)
    New York : Wiley-Blackwell
    Biopolymers 7 (1969) 
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    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
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    Conformation of polypeptide in the, helix-coil transition region (1969)
    New York : Wiley-Blackwell
    Biopolymers 7 (1969), S. 433-434 
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    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: No. Abstract
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    Experimental thermodynamics of the helix-random coil transition. II. Calorimetric measurement of transition enthalpies of poly A in acid aqueous solution (1969)
    New York : Wiley-Blackwell
    Biopolymers 7 (1969), S. 423-431 
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    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The enthalpy change accompanying the double helix-coil transition of polyriboadenylic acid (poly A) in aqueous solution has been measured optically and calorimetrically in the pH range 5.7-4.5. The course of this cooperative transition was followed optically by measuring changes in ultraviolet absorption as a function of temperature at different pH values, and calorimetrically by determining the heat capacity of the solution through the transition region. From the latter measurements, the enthalpy of transition was calculated. It is shown, that ΔH is dependent on pH as it is expected from the influence of protonation of the double helix of poly A.
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    Mechanical properties of poly(glutamic acid) in aqueous solution (1969)
    New York : Wiley-Blackwell
    Biopolymers 7 (1969), S. 453-458 
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    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The interaction between ionizable carboxyl groups and the conformation of poly-(glutamic acid) (PGA) in aqueous solution were investigated by the mechanical method. The dynamic rigidity of the PGA solution has a maximum value at the pH corresponding to about 50% neutralization point. This may be due to establishing of a maximum attractive force by proton/charge fluctuation between ionizable carboxyl groups at that pH. The dynamic viscosity has a sharp change in the region of pH 5.5-6.5. It is suggested that this behavior is due to the helix-coil transition.
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    Proton relaxation in aqueous DNA solutions (1969)
    New York : Wiley-Blackwell
    Biopolymers 7 (1969), S. 447-452 
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    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: By use of D2O we found that the shortening of the longitudinal proton relaxation time which occurs in the investigated aqueous yeast DNA solutions (≦ 2.4% with 2% protein) was not based on a hydration effect, but was caused by magnetic impurities only. An estimate shows that the mobility of the hydrated water molecules is reduced by less than two orders of magnitude in comparison with the free water molecules.
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    Method of determining the relative stability of different conformational states of biological macromolecules (1969)
    New York : Wiley-Blackwell
    Biopolymers 7 (1969), S. 435-445 
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    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A general approach to the determination of relative stability of any pair of con-formational states of biological macromolecules or their complexes (in particular, to the determination of relative stability of native and disordered states of the macromolecule) has been suggested. For determining the free energy difference of the two states under the conditions when one of them is considerably more advantageous than the other, it is necessary for the macromolecule to be influenced by the transforming agent which levels free energies of both the conformational states, and to determine the external parameter derivative of the free energy difference in the region of the conformational transition induced by the change in this parameter. If the character of the dependence of this derivative on the external parameter (temperature, solvent composition, etc.) is known, then this allows the determination of the free energy difference of the two states under the conditions considered, even including conditions far from the transition region. The value of the derivative of the free energy difference in the transition region in many cases can be measured directly (in particular, when using calorimetry), while in cases when a direct measurement of the derivative is impossible, it can often he estimated experimentally from the steepness of the conformational transition. The methods of this estimation and also a possible character of the change of the considered derivative during variation of the external parameter are considered for the case when the transforming agent is one of the components of the solvent and, consequently, the derivative of the free energy difference is equal to the difference of number of molecules of this component hound with the macromolecule in two conformational states.
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    Heats of fusion of various amounts of absorbed water in wool keratin (1969)
    New York : Wiley-Blackwell
    Biopolymers 7 (1969), S. 459-474 
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    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Wool-water systems, at water contents above a threshold value of 22.7 g of water per 100 g of wool keratin, yielded curves of specific heat against temperature showing peaks that are ascribed to the fusion of the absorbed water. Integral and incremental heats of fusion were obtained. Integral heats increase as the square of the water content above the threshold, and incremental heats follow a linear relationship. The incremental heat reaches a value of 55 cal/absorbed water, at the saturation water content, at 0°C of 33.9 g of water per 100 g of keratin. The state of the absorbed water is discussed. Clustering of water molecules takes place only above the threshold water content.
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    Raman spectroscopy of poly-L-proline in aqueous solution (1969)
    New York : Wiley-Blackwell
    Biopolymers 8 (1969), S. 173-179 
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    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The laser-excited Raman spectra have been obtained of poly-L-proline in the solid and aqueous solution. In the solid state, the Raman spectra are of the form II which is the left-handed helical form with the peptide bonds in the trans configuration. Dissolution of this form in water produced only minor changes in the Raman spectra. The differences in frequencies produced by dissolving the polymer are associated with vibrational modes of the pyrrolidine ring as indicated by comparison with the Raman spectrum of L-proline. The similarity of the Raman spectra dictates that, the conformations of poly-L-proline chain are similar in the solid and aqueous solution.
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    URL: http://dx.doi.org/10.1002/bip.1969.360080204
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    Mise en évidence d'une relation entre la longueur el la constante de sédimentation des molécules de DNA en solution (1969)
    New York : Wiley-Blackwell
    Biopolymers 8 (1969), S. 181-185 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A comparative study on sonicated calf thymus DNA was made by using electron microscopy and sedimentation methods. The length distribution, established by electron microscopy by using Kleinschmidt's technique, is related to the sedimentation curve obtained with the same solution. The relation permits a determination of the sedimentation constant corresponding to a given length up to 8000 Å and vice versa.
    Additional Material: 2 Ill.
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    URL: http://dx.doi.org/10.1002/bip.1969.360080205
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  • 88
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    Optical anisotropy of synthetic polynucleotides. II. Calculation of π-electron polarizability of uracil and adenine (1969)
    New York : Wiley-Blackwell
    Biopolymers 8 (1969), S. 199-216 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The π-electron polarizability of conjugated base molecules such as uracil and adenine are calculated semiempirically by using molecular orbital theories. The Hüekel method and the self-consistent-field (SCF) theory are used in the present calculation. The theoretical values obtained by the Hüekel method are consistently larger than the experimental results. The values obtained by the SCF theory are in good agreement with observed values. It is clearly demonstrated that the SCF method is better than the Hükel method for the calculation of polarizability. The polarizability of the adenine-uracil pair is calculated as the sum of the polarizabilities of adenine and uracil and also calculated by solving the eigenvalue problem for the A-U pair as an entity. The second method gives considerably larger polarizability than the first method. Discussions are presented and it is demonstrated that the second method is the correct way to calculate the polarizability of the A-U pair.
    Additional Material: 5 Ill.
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    URL: http://dx.doi.org/10.1002/bip.1969.360080207
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    Reversibility of thermal transitions in proteins: Racemization and aggregation as factors in the reversible denaturation of a soluble keratin derivative (SCMKA) (1969)
    New York : Wiley-Blackwell
    Biopolymers 8 (1969), S. 187-198 
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    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The reversibility of the thermal denaturation of a low-sulfur fraction of solubilized wool keratin (SCMKA) has been studied under a variety of conditions of time, protein concentration, and pH. Two types of irreversibility for the transition have been encountered. One of these is associated with an aggregation of the protein on denaturation to give a product which may contain elements of a β conformation. This type of irreversibility is favored by high protein concentration, and the original conformation may in fact be regained if the aggregated structure is broken down by a solvent such as 8M urea and the urea subsequently removed by dialysis. The other type of irreversibility appears to be due to racemization of the protein. It does not seem to be dependent on protein concentration and is apparent only at temperatures beyond the actual transition range (∼40-65°C) at pH values below 11, At pH 12, however, racemization appears to proceed slowly even at 4°C. The thermal transition at pH 9 and pH 10 has been shown to be multistage in nature. Over the pH range 9-12 there is a progressive decrease in thermal stability with increase of pH. Addition of NaCl at pH 10 leads to an increase in thermal stability of the molecule.
    Additional Material: 2 Ill.
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    URL: http://dx.doi.org/10.1002/bip.1969.360080206
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  • 90
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    Studies of the optical properties of the proflavine-DNA complex (1969)
    New York : Wiley-Blackwell
    Biopolymers 8 (1969), S. 217-235 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: We report studies of the optical properties of the proflavine-DNA complex, using absorbance and circular dichroism spectroscopy. From comparison of the absorption spectra of proflavine complexed with calf thymus and T2 DNA, we conclude that stacking of the dyes external to the double helix is comparatively much weaker with T2 DXA, probably because of its glucosylation. Several sources are found for the circular dichroism induced in proflavine when it is complexed with DNA. There is a relatively weak circular dichroism induced when the dye is infinitely dilute on the DNA lattice; this presumably arises from the environmental asymmetry of the binding site. Stronger circular dichroism effects are induced by interaction of intercalated and stacked dyes; studies with T2 DNA, for which stacking seems to be blocked, permit a tentative resolution of effects due to the two modes of binding. One recurring theme of these studies is the observation that the optical properties are quite dependent on environment. The most dramatic example is a strong variation with salt concentration of the amplitude of the circular dichroism induced in the isolated (intercalated) monomer by the surrounding DNA. This suggests that the structure of the intercalated complex is quite sensitive to external conditions.
    Additional Material: 17 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.1969.360080208
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  • 91
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    Binding of alcohols to the peptide CO-group of poly-L-proline in the I and II conformation. I. Demonstration of the binding by infrared spectroscopy and optical rotatory dispersion (1969)
    New York : Wiley-Blackwell
    Biopolymers 8 (1969), S. 237-246 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The binding of n-butanol, benzyl alcohol, and trifluoroethanol to polyproline I (all peptide bonds in cis configuration) and II (all peptide bonds in trans configuration) has been demonstrated. It was found by infrared spectroscopy that hydrogen bonds are formed between the CO groups of the polymer and the OH groups of the alcohols. Their strength increases from n-butanol via benzyl alcohol to trifluoroethanol. The binding of benzyl alcohol could also be followed by optical rotatory dispersion due to the fact that the electronic transitions of the benzyl ring are rendered optically active by the binding to the polymer.
    Additional Material: 2 Ill.
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    URL: http://dx.doi.org/10.1002/bip.1969.360080209
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  • 92
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    Helix-random coil phase transitions in polypeptide systems with random defects: A statistical mechanical model (1969)
    New York : Wiley-Blackwell
    Biopolymers 8 (1969), S. 259-274 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The statistical mechanics of the effect of a small number of randomly occurring defects on the melting of a polypeptide chain is developed by using a perturbation of Boltzmann factors technique. The method is illustrated by an example where a second-component impurity contributes more to the energy of the hydrogen-bonded superstructure of the polypeptide chain than does the dominant amino acid residue, resulting in the overall destabilization of the helical conformation.
    Additional Material: 1 Ill.
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    URL: http://dx.doi.org/10.1002/bip.1969.360080211
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    Deformation behavior of erythrocyte ghost (1969)
    New York : Wiley-Blackwell
    Biopolymers 8 (1969), S. 275-287 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Biological membranes consist mainly of lipids and proteins. At present, the structure of the lipid phase appears to be established, but hypotheses on the molecular organization of the protein are difficult to support. Thus the deformation behavior of whole human erythrocyte ghosts, ghosts after the selective removal of lipids and ghosts stripped of lipids as well as nonlipid components have been examined in the hope of securing indirect information on the organization of the protein. It has been found that large localized deformations result in partial membrane failure and long uniformly wide fibrils, frequently in excess of 3000 Å, are drawn across the rupture. These data are interpreted in terms of currently favored membrane models and the possibility of a fibrous membrane framework consisting predominantly of protein is reviewed. The behavior of the membrane in its various stages of extraction is compared and contrasted to that of synthetic polymer films of known organization.
    Additional Material: 6 Ill.
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    URL: http://dx.doi.org/10.1002/bip.1969.360080212
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    Helix-coil transition of poly-N5-(3-hydroxypropyl)-L-glutamine: Thermodynamic and statistical analysis (1969)
    New York : Wiley-Blackwell
    Biopolymers 8 (1969), S. 247-257 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The thermally induced conformational changes of poly-N5-(3-hydroxypropyl)-L-glutamine in water and in methanol-water (3:7 v/v) have been analyzed in terms of the Lifson-Roig theory. The transitions in both solvents can be described by using v = 0.017. The thermodynamic parameters for the random coil-to-helix transition of one amino acid residue at room temperature were found to be: in water, ΔH = - 130 cal/mole and ΔS = - 0.45 e.u.; in methanol-water (3:7 v/v), ΔH = - 170 cal mole and ΔS = - 0.45 e.u. The size distribution of helical segments is broad, and the results of numerical calculations are presented for three degrees of polymerization (DP = 100, 300, and 750).
    Additional Material: 8 Ill.
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    URL: http://dx.doi.org/10.1002/bip.1969.360080210
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    Extrinsic cotton effects of 9-aminoacridine bound to deoxyribonucleic acid and poly(α, L-glutamic acid) (1969)
    New York : Wiley-Blackwell
    Biopolymers 8 (1969), S. 289-292 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.1969.360080213
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    Molecular weight and dimensions of phycocyanin monomer and aggregates (1969)
    New York : Wiley-Blackwell
    Biopolymers 8 (1969), S. 293-295 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.1969.360080214
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    Molecular weight and shape of phycocyanin monomer and aggregates (1969)
    New York : Wiley-Blackwell
    Biopolymers 8 (1969), S. 297-299 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.1969.360080215
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    Equilibrium sedimentation of a polyelectrolyte in a density gradient of a low-molecular weight electrolyte. I. DNA in CsCl (1969)
    New York : Wiley-Blackwell
    Biopolymers 8 (1969), S. 303-304 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.1969.360080217
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    Cesium chloride density distributions in preparative equilibrium ultracentrifugation and computer program (1969)
    New York : Wiley-Blackwell
    Biopolymers 8 (1969), S. 301-301 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.1969.360080216
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    Nmr studies in solution of poly-L-proline (1969)
    New York : Wiley-Blackwell
    Biopolymers 8 (1969), S. 304-304 
    Details
    ISSN: 0006-3525
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bip.1969.360080218
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