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  • Artikel  (67.927)
  • Molecular Diversity Preservation International  (45.224)
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  • 2020-2023
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  • 1
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    The Uncommon Active Site of D-Amino Acid Transaminase from Haliscomenobacter hydrossis: Biochemical and Structural Insights into the New Enzyme (2021)
    Molecular Diversity Preservation International
    Details
    Publikationsdatum: 2021-08-20
    Beschreibung: Among industrially important pyridoxal-5’-phosphate (PLP)-dependent transaminases of fold type IV D-amino acid transaminases are the least studied. However, the development of cascade enzymatic processes, including the synthesis of D-amino acids, renewed interest in their study. Here, we describe the identification, biochemical and structural characterization of a new D-amino acid transaminase from Haliscomenobacter hydrossis (Halhy). The new enzyme is strictly specific towards D-amino acids and their keto analogs; it demonstrates one of the highest rates of transamination between D-glutamate and pyruvate. We obtained the crystal structure of the Halhy in the holo form with the protonated Schiff base formed by the K143 and the PLP. Structural analysis revealed a novel set of the active site residues that differ from the key residues forming the active sites of the previously studied D-amino acids transaminases. The active site of Halhy includes three arginine residues, one of which is unique among studied transaminases. We identified critical residues for the Halhy catalytic activity and suggested functions of the arginine residues based on the comparative structural analysis, mutagenesis, and molecular modeling simulations. We suggested a strong positive charge in the O-pocket and the unshaped P-pocket as a structural code for the D-amino acid specificity among transaminases of PLP fold type IV. Characteristics of Halhy complement our knowledge of the structural basis of substrate specificity of D-amino acid transaminases and the sequence-structure-function relationships in these enzymes.
    Digitale ISSN: 1420-3049
    Thema: Chemie und Pharmazie
    Publiziert von Molecular Diversity Preservation International
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  • 2
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    Electrical Stress on the Medium Voltage Medium Frequency Transformer (2021)
    Molecular Diversity Preservation International
    Details
    Publikationsdatum: 2021-08-19
    Beschreibung: This paper proposes an equivalent circuit model to obtain the transient electrical stress quantitatively in medium voltage medium frequency transformers in modern power electronics. To verify this model, transient simulation is performed on a 1.5 kV/1 kHz transformer, revealing voltage overshoot quantitatively between turns and layers of the transformer’s HV winding. Effects of rise time of the input pulse voltage, stray capacitance of the winding insulation, and their interactions on the voltage overshot magnitude are presented. With these results, we propose limiting the voltage overshoot and, thereafter, enhancing medium voltage medium frequency transformer’s insulation capability, which throws light on the transformer’s insulation design. Additionally, guidance on the future studies on aging and endurance lifetime of the medium voltage medium frequency transformer’s insulation could be given.
    Digitale ISSN: 1996-1073
    Thema: Energietechnik
    Publiziert von Molecular Diversity Preservation International
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  • 3
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    Review of the Potential of Consumer Neuroscience for Aroma Marketing and Its Importance in Various Segments of Services (2021)
    Molecular Diversity Preservation International
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    Publikationsdatum: 2021-08-19
    Beschreibung: In the current era of a strongly competitive business environment, it is more difficult for companies to attract customers. Consumer neuroscience has growing potential here, as it reveals internal consumer preferences by using innovative methods and tools, which can effectively examine consumer behavior and attract new customers. In particular, smell has a great ability to subconsciously influence customers and, thus, support profitability. This paper examines the importance of consumer neuroscience and its modern technologies used for exploring human perceptions to influence customers and benefit from the aromatization of business spaces. We focused our analysis on various service sectors. Despite the potential of the examined issue, there are a limited number of studies in the field of service providers that use neuroscience tools to examine the effect of aromas on human emotions. Most studies took place in laboratory conditions, and the used methodological procedures varied widely. Our analysis showed that, in spite of the positive impact of aromatization in the majority of aromatized spaces, service companies still do not use the potential of consumer neuroscience and aroma marketing to a sufficient degree. Innovative methods and tools, in particular, are still very underused.
    Digitale ISSN: 2076-3417
    Thema: Allgemeine Naturwissenschaft
    Publiziert von Molecular Diversity Preservation International
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  • 4
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    Measuring Ocean Surface Current in the Kuroshio Region Using Gaofen-3 SAR Data (2021)
    Molecular Diversity Preservation International
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    Publikationsdatum: 2021-08-20
    Beschreibung: The Kuroshio is the strongest warm current in the western North Pacific, which plays a crucial role in climate and human activities. In terms of this, the accurate acquisition of ocean surface current velocity and direction in the Kuroshio region is of great research value. Gaofen-3 synthetic aperture radar (SAR) provides data support for the study of ocean surface current measurements in the Kuroshio region, but no relevant experimental result has been published yet. In this paper, four available stripmap mode SARs’ data acquired by Gaofen-3 in the Kuroshio region are used for measuring the ocean surface current field. In general, the Doppler centroid anomaly (DCA) estimation is a common method to infer ocean surface currents from single-antenna stripmap data, but only the radial velocity component can be retrieved. In order to measure current vectors, a novel method combining the sub-aperture processing and the least squares (LS) technology is suggested and demonstrated by applying to the Gaofen-3 SAR data processing. The experiment’s results agree well with model-derived ocean current data, indicating that the Gaofen-3 SAR has the capability to accurately retrieve the ocean surface current field in the Kuroshio region and motivate further research by providing more data.
    Digitale ISSN: 2076-3417
    Thema: Allgemeine Naturwissenschaft
    Publiziert von Molecular Diversity Preservation International
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  • 5
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    Steam Explosion-Assisted Extraction of Protein from Fish Backbones and Effect of Enzymatic Hydrolysis on the Extracts (2021)
    Molecular Diversity Preservation International
    Details
    Publikationsdatum: 2021-08-20
    Beschreibung: The development of an efficient pretreatment, prior to enzymatic hydrolysis, is a good strategy for the sustainable use of refractory fish byproducts. This study compared hydrothermal pretreatments at 159 °C for 2 min, followed by water extraction (steam explosion-assisted extraction, SE) and 121 °C for 70 min (hot-pressure extraction, HPE), for the recovery of proteins from fish backbones. The effect of enzymatic hydrolysis on the properties of the obtained fish bone protein (FBP) was also evaluated. The results demonstrated that FBP had high contents of protein (81.09–84.88 g/100 g) and hydroxyproline (70–82 residues/1000 residues). After hydrolysis with Flavourzyme, for 3 h, the FBP hydrolysates that were pretreated with SE (SFBP-H) exhibited a better degree of hydrolysis (DH) and nitrogen recovery (NR), and a higher level of umami taste free amino acids (151.50 mg/100 mL), compared with the HPE-treated samples. The obtained SFBP-H mainly distributed below 3000 Da and had strong scavenging effects on 1,1-diphenyl-2-picrylhydrazy (DPPH) (IC50 = 4.24 mg/mL) and 2,2-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) (IC50 = 1.93 mg/mL) radicals. Steam explosion-assisted extraction is a promising route for recovering proteins from native fish bone materials, and improving the flavor and antioxidant activity of the hydrolysates.
    Digitale ISSN: 2304-8158
    Thema: Chemie und Pharmazie
    Publiziert von Molecular Diversity Preservation International
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  • 6
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    Long-Term Impact of COVID-19 on Mental Health among the General Public: A Nationwide Longitudinal Study in China (2021)
    Molecular Diversity Preservation International
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    Publikationsdatum: 2021-08-20
    Beschreibung: COVID-19 might have long-term mental health impacts. We aim to investigate the longitudinal changes in mental problems from initial COVID-19 peak to its aftermath among general public in China. Depression, anxiety and insomnia were assessed among a large-sample nationwide cohort of 10,492 adults during the initial COVID-19 peak (28 February 2020 to 11 March 2020) and its aftermath (8 July 2020 to 8 August 2020) using the Patient Health Questionnaire-9, Generalized Anxiety Disorder-7, and Insomnia Severity Index. We used generalized estimating equations and linear mixed models to explore factors associated with long-term mental health symptoms during COVID-19. During the five months, mental health symptoms remained consistently elevated (baseline 46.4%; follow-up 45.1%). Long-term depression, anxiety and insomnia were associated with several personal and work-related factors including quarantine (adjusted OR for any mental health symptoms 1.31, 95%CI 1.22–1.41, p 〈 0.001), increases in work burden after resuming work (1.77, 1.65–1.90, p 〈 0.001), occupational exposure risk to COVID-19 (1.26, 1.14–1.40, p 〈 0.001) and living in places severely affected by initial COVID-19 peak (1.21, 1.04–1.41, p = 0.01) or by a COVID-19 resurgence (1.38, 1.26–1.50, p 〈 0.001). Compliance with self-protection measures, such as wearing face masks (0.74, 0.61–0.90, p = 0.003), was associated with lower long-term risk of mental problems. The findings reveal a pronounced and prolonged mental health burden from the initial COVID-19 peak through to its aftermath in China. We should regularly monitor the mental health status of vulnerable populations throughout COVID-19.
    Print ISSN: 1661-7827
    Digitale ISSN: 1660-4601
    Thema: Energietechnik , Medizin
    Publiziert von Molecular Diversity Preservation International
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  • 7
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    Recent Development in the Design of Wind Deflectors for Vertical Axis Wind Turbine: A Review (2021)
    Molecular Diversity Preservation International
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    Publikationsdatum: 2021-08-20
    Beschreibung: Developments in the design of wind turbines with augmentation are advancing around the globe with the goal of generating electricity close to the user in built-up areas. This is certain to help lessen the power generation load as well as distribution and transmission network costs by reducing the distance between the user and the power source. The main objectives driving the development and advancement of vertical-axis wind turbines are increasing the power coefficient and the torque coefficient by optimizing the upstream wind striking on the rotor blades. Unlike horizontal-axis wind turbines, vertical axis turbines generate not only positive torque but also negative torque during operation. The negative torque generated by the returning blade is a key issue for vertical-axis wind turbines (VAWTs) that is counterproductive. Installation of wind deflectors for flow augmentation helps to reduce the negative torque generated by the returning blades as well as enhance the positive torque by creating a diversion in the upstream wind towards the forwarding blade during operation. This paper reviews various designs, experiments, and CFD simulations of wind deflectors reported to date. Optimization techniques for VAWTs incorporating wind deflectors are discussed in detail. The main focus of the review was on the installation position and orientation of the deflectors and their potential contribution to increasing the power coefficient. Topics for future study are suggested in the conclusion section of the paper.
    Digitale ISSN: 1996-1073
    Thema: Energietechnik
    Publiziert von Molecular Diversity Preservation International
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  • 8
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    Food Safety of Consuming Black Soldier Fly (Hermetia illucens) Larvae: Microbial, Heavy Metal and Cross-Reactive Allergen Risks (2021)
    Molecular Diversity Preservation International
    Details
    Publikationsdatum: 2021-08-20
    Beschreibung: Black soldier fly (Hermetia illucens) larvae (BSFL) are a promising, sustainable source of nutrients, however, there is limited knowledge regarding the food safety of consuming BSFL. This study determined the safety of consuming BSFL for direct human consumption in terms of microbial, heavy metal and allergen content. Microbial counts were determined using ISO (International Organization for Standardization) methods, heavy metals were determined using inductively coupled plasma mass spectrometry and allergens were determined via Orbitrap mass spectrometry and ELISA (enzyme-linked immunosorbent assay) kits. Feed and killing method influenced the presence of Bacillus cereus (p = 0.011), and only the killing method influenced Escherichia coli (p 〈 0.00) and total viable count (TVC) (p 〈 0.00). Blanching resulted in a 3-log reduction in E. coli and a 3.4 log reduction in the TVC counts. Salmonella spp. and Listeria spp. were not detected in the BSFL samples. Heavy metals were detected although they were below maximum legal limits. Cross-reactive allergens, tropomyosin and arginine kinase, were detected in the BSFL samples, although the clinical significance requires research. The feed fed to the BSFL and blanching were found to influence the safety of consuming BSFL, highlighting the importance of incorporating sufficient decontamination steps, such as blanching, to ensure food safety.
    Digitale ISSN: 2304-8158
    Thema: Chemie und Pharmazie
    Publiziert von Molecular Diversity Preservation International
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  • 9
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    Perception of the Movement Control Order during the COVID-19 Pandemic: A Qualitative Study in Malaysia (2021)
    Molecular Diversity Preservation International
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    Publikationsdatum: 2021-08-20
    Beschreibung: Malaysia implemented its first Movement Control Order (MCO) during the early phase of the COVID-19 pandemic to slow the transmission of the virus. This study aimed to explore the public perception of the MCO implementation and people’s experiences during this period. The study employed qualitative explorative in-depth interviews conducted with 23 Malaysian adults from various demographic backgrounds. Thematic analysis was performed using NVivo 12. Three main themes were identified: a period of information surge, heterogeneous emotional response, and attempts to adapt. During the MCO, the participants obtained information from multiple platforms. They suggested the need for clear and repeated instructions to avoid confusion and misinformation. They also acknowledged the importance of the MCO in breaking the chain of transmission and safeguarding high-risk groups; however, they also expressed that stricter enforcement from the authorities was warranted. The changes in the participants’ work–life routines, lack of physical interaction, and uncertainty about their health and the economy due to the MCO negatively impacted their psychological states. Despite these challenges, the participants attempted to adapt to life under the MCO in different ways. The findings imply that during a crisis, the public tends to seek clear and reliable information, experience emotional turmoil, and adapt to changes. The MCO implementation can be improved through an effective communication strategy and efforts to battle misinformation.
    Print ISSN: 1661-7827
    Digitale ISSN: 1660-4601
    Thema: Energietechnik , Medizin
    Publiziert von Molecular Diversity Preservation International
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  • 10
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    Ultimate Compressive Strains and Reserves of Bearing Capacity of Short RC Columns with Basalt Fiber (2021)
    Molecular Diversity Preservation International
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    Publikationsdatum: 2021-08-19
    Beschreibung: Increasing the bearing capacity of reinforced concrete structures, reducing material consumption, and ensuring quality are critical in modern construction. The article presents an experimental study of the ultimate compressive strains of short fiber basalt reinforced concrete columns and provides recommendations for increasing the bearing capacity using steel reinforcement bars with greater strength. The columns were tested in an upright position using a hydraulic press. Strains were measured with dial indicators and a strain gauge station. It was shown that the addition of 10% coarse basalt fiber increased the ultimate compressibility of concrete on ordinary crushed stone by 19.8%, and expanded clay concrete by 26.1%, which led to the strain hardening of concrete under compression by 9.0% and 12%, respectively. Ultimate compressive strains in fiber-reinforced concrete short columns with combined reinforcement increased 1.42 times in columns on a lightweight aggregate and 1.19 times on heavy aggregate. An increase in the ultimate compressibility of concrete makes it possible to use steel reinforcement with greater strength in compressed elements as the concrete crushing during compression occurs primarily due to the reaching of critical values by tensile stresses in the transverse direction. This makes it possible to manufacture structures with a higher load-bearing capacity and less material consumption. A practical example of the application of the proposed approach is given.
    Digitale ISSN: 2076-3417
    Thema: Allgemeine Naturwissenschaft
    Publiziert von Molecular Diversity Preservation International
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