Coffee is a crop of global importance, and it is especially important in countries such as Peru. However, the presence of the pest
Hypothenemus hampei represents a significant challenge with a notable economic impact. This study addresses this challenge using entomopathogenic fungi such
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Coffee is a crop of global importance, and it is especially important in countries such as Peru. However, the presence of the pest
Hypothenemus hampei represents a significant challenge with a notable economic impact. This study addresses this challenge using entomopathogenic fungi such as
Beauveria peruviensis and
Metarhizium sp. The compatibility of three strains of
Beauveria peruviensis (F5, P19, and P4) and seven strains of
Metarhizium sp. (MMR-M1, LLM-M2, MHR-M4, PMR-M12, MMR-M15, TOR-M16, and GOR-M18) was evaluated for approximately 2 months. A total of 14 treatments were designed, each consisting of one strain of
B. peruviensis and one strain of
Metarhizium sp. The Skott–Knott test (
p ≤ 0.05) revealed that strain LLM-M2 (
Metarhizium sp. strain) had the highest conidial production (3.75 × 10
7 conidia/mL). Except for T6 (MMR-M1/F5), which showed a mutual growth type interaction (type A), all other strain combinations showed a type B interaction (mutual inhibition by contact or separation between colony margins (<2 mm)). The combination with the highest germination rate was T10 (MHR-M4/F5) at 89%. In addition, the pathogenicity of the combined strains was evaluated, showing a direct correlation with mortality and mycosis development in the coffee berry borer in treatments T1 (PMR-M12/P19), T10 (MHR-M4/F5), and T11 (MMR-M15/P19), reaching 100% mortality at 72 h with grade 4 mycosis. Regarding mycelial growth, treatments T1 (PMR-M12/P19), T4 (MMR-M1/P19), and T12 (GOR-M18/P19) reached the highest percentages, between 85.8% and 83.10% at 240 h. This study demonstrates the feasibility of using native strains of
B. peruviensis and
Metarhizium sp. as a biocontrol strategy against the coffee berry borer in the Amazon department, presenting them as an alternative to traditional chemical methods.
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