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  • Analytical Chemistry and Spectroscopy  (101)
  • conservation  (53)
  • Wiley-Blackwell  (101)
  • Springer  (53)
  • National Academy of Sciences
  • 2000-2004  (154)
  • 1985-1989
  • 1960-1964
  • 1950-1954
  • 2000  (154)
Collection
Keywords
Publisher
  • Wiley-Blackwell  (101)
  • Springer  (53)
  • National Academy of Sciences
Years
  • 2000-2004  (154)
  • 1985-1989
  • 1960-1964
  • 1950-1954
Year
  • 1
    Electronic Resource
    Electronic Resource
    A set of microsatellite loci for the hairy-nosed wombats (Lasiorhinus krefftii and L. latifrons) (2000)
    Springer
    Conservation genetics 1 (2000), S. 89-92 
    Details
    ISSN: 1572-9737
    Keywords: conservation ; marsupial ; remote censusing ; microsatellites ; wombat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1010146107009
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  • 2
    Electronic Resource
    Electronic Resource
    Farmers indigenous practices for conserving Garcinia kola and Gnetum africanum in southern Cameroon (2000)
    Springer
    Agroforestry systems 48 (2000), S. 289-302 
    Details
    ISSN: 1572-9680
    Keywords: conservation ; humidforest zone ; native plants ; non-timber forest products
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Traditional approaches to conservation of Garcinia kola and Gnetum africanum were assessed by a survey of 15 villages (three per province) in the humid forest zone of southern Cameroon. Four major land use systems [evergreen forest, degraded forest, bush fallow (10 years and over) and food crop fields] were identified as major niches for the species. The distribution of the plant stands varied from province to province. Gnetum africanum is intensively harvested (up to four times per week throughout the year) and reported to generate substantial income (averaging US$2,630 per household per annum). Indigenous practices used by farmers to protect the species include selective clearing during land preparation for cropping, sustainable bark harvesting of stands in wild population, transplantation of wildings, artificial propagation and recognition of individual property ownership on certain wild stands of G. kola.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1006393709637
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  • 3
    Electronic Resource
    Electronic Resource
    Occurrence of Larca lata H.J. Hansen (Pseudoscorpionida: Garypidae) and Allochernes wideri C.L. Koch (Pseudoscorpionida: Chernetidae) in tree hollows in relation to habitat quality and density (2000)
    Springer
    Journal of insect conservation 4 (2000), S. 23-31 
    Details
    ISSN: 1572-9753
    Keywords: Allochernes wideri ; conservation ; hollow tree ; Larca lata ; pseudoscorpion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Nature of Science, Research, Systems of Higher Education, Museum Science
    Notes: Abstract Many beetles associated with old trees are on national red lists, but pseudoscorpions living in similar habitats have received little attention. This study reports the habitat and occurrence patterns of two species of pseudoscorpions living in hollow trees. Their occurrence has been assessed by sieving wood mould from 274 oaks in southeastern Sweden and from museum specimens collected in Sweden. Larca lata is confined to hollow oaks with a large girth and a plentiful supply of wood mould. Allochernes wideri is much less particular about wood mould volume, trunk diameter and tree species. Larca lata inhabits hollow trunks with characteristics that are typical of very old trees, whereas A. wideri predominantly occurs in trunks in an earlier stage of hollow formation. Larca lata was almost exclusively found in larger assemblages of hollow oaks, which suggests long-term survival may be difficult when the network of suitable hollow trees is too sparse. Larca lata is a rare species in Europe and probably vulnerable to extinction, since it is dependent on a habitat which has declined severely in the last few centuries.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009682722905
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  • 4
    Electronic Resource
    Electronic Resource
    Genetic Status and Fluctuating Asymmetry in an Endangered Population of the Moth Dysauxes ancilla L. (Lepidoptera: Ctenuchidae) (2000)
    Springer
    Journal of insect conservation 4 (2000), S. 93-98 
    Details
    ISSN: 1572-9753
    Keywords: allozymes ; fluctuating asymmetry ; Dysauxes ancilla ; conservation ; Sweden
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Nature of Science, Research, Systems of Higher Education, Museum Science
    Notes: Abstract Genetic status and fluctuating asymmetry (FA) were assessed in a small, isolated and endangered population of the moth Dysauxes ancilla in Sweden. A sample from the German population, within the continuous breeding area of the species, was used for comparison. The levels of FA were significantly higher in the Swedish population, indicating a reduced ability to withstand developmental stress. Two polymorphic loci showed significantly higher variation in the Swedish population, indicating that there are no serious effects on genetic factors. Therefore, it is suggested that the increased level of FA in the Swedish population is due to the stress of living in an ecologically marginal habitat. The Swedish population is a northern outpost separated from the continuous distribution area of species and environmental stress caused by variable and extreme abiotic factors, for example climatic conditions, could explain a higher FA. However, it is still an open question if a higher FA from environmental stress also constitutes an increased extinction risk.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009648622775
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  • 5
    Electronic Resource
    Electronic Resource
    Comparative Biogeography of Grasshoppers (Orthoptera: Acrididae) in North America and Siberia: Applications to the Conservation of Biodiversity (2000)
    Springer
    Journal of insect conservation 4 (2000), S. 161-172 
    Details
    ISSN: 1572-9753
    Keywords: Acrididae ; conservation ; distribution ; diversity ; rarity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Nature of Science, Research, Systems of Higher Education, Museum Science
    Notes: Abstract The objective of this study was to apply knowledge of holarctic grasshopper biogeography in representative, temperate ecosystems (Russia, South Siberia, Sayans region; 685,000 km2 and USA, Wyoming; 272,000 km2) to develop a comparative basis for understanding and conserving insect biodiversity. Maps of species distributions and vegetation zones were digitized, and a Geographic Information System was used to identify habitats with the greatest biodiversity and to characterize this diversity as a function of selected attributes. With respect to subfamilies, the greatest differences were in cold, mesic zones, where gomphocerines and melanoplines were dominant in Sayans and Wyoming, respectively. In terms of mobility, the Sayans has more flightless species and individuals, with the taiga supporting the greatest frequency of flightless acridids in both countries. With regard to feeding types, the diversity and richness of graminivores and forbivores were similar in the two regions, but mixed feeders were much more frequent in Wyoming. In the Sayans and Wyoming, pest species were most common in boreal and prairie zones, respectively. Ecoregions with a high diversity of pests also supported a high diversity of rare species. Shrub and desert zones supported many rare species in both countries. Thus, in terms of conservation, the Sayans' acridofauna appears to be at greater risk in terms of ecological vulnerability; acridid biodiversity is dispersed among habitats, with high frequencies of flightless and oligophagous species. The acridofauna of Wyoming could be conserved in fewer habitats than in the Sayans, but these habitats are subject to considerable human disturbance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1009618425473
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  • 6
    Electronic Resource
    Electronic Resource
    Status and Breeding Sites of Three Presumed Endangered Scottish Saproxylic Syrphids (Diptera, Syrphidae) (2000)
    Springer
    Journal of insect conservation 4 (2000), S. 215-223 
    Details
    ISSN: 1572-9753
    Keywords: conservation ; red-listed ; flight period ; distribution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Nature of Science, Research, Systems of Higher Education, Museum Science
    Notes: Abstract From 1987 to 1999 efforts were made to understand the status and breeding sites of three presumed endangered flies in Britain: Blera fallax (Linnaeus), Hammerschmidtia ferruginea (Fallén) and Callicera rufa Schummel (Diptera, Syrphidae). Historical data on flight periods, localities and breeding sites were collated from the literature and captured specimens in museums and other collections. Using these data, life cycles were investigated, and cited and other localities searched for adults and early stages. Looking for early stages was more productive than looking for adults. B. fallax is the most endangered. It has declined in abundance, is restricted to two localities and, in 1999, breeding sites were destroyed at one of these localities. In contrast C. rufa is widespread and not uncommon throughout northern Scotland. H. ferruginea is not as endangered as B. fallax but adverse factors such as habitat destruction affect most of its sites.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1011380316156
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  • 7
    Electronic Resource
    Electronic Resource
    Polyphyly of mtDNA lineages in the Russian sturgeon, Acipenser gueldenstaedtii: forensic and evolutionary implications (2000)
    Springer
    Conservation genetics 1 (2000), S. 81-88 
    Details
    ISSN: 1572-9737
    Keywords: caviar ; conservation ; genetics ; mtDNA ; sturgeon
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Molecular species identification methods are an important component of CITES monitoring programs for trade in sturgeon and caviar. To date, obtaining molecular evidence for distinguishing caviar from four closely related Eurasian sturgeon species Acipenser baerii (Siberian sturgeon), A. gueldenstaedtii (osetra), A. persicus (Persian sturgeon), A. naccarii (Italian sturgeon) remains problematic. Using approximately 2.3 kb of mtDNA sequence data (cytochrome b, NADH5, control region), we find this to be attributable to the polyphyletic nature of these mitochondrial DNA markers in the Russian sturgeon, A. gueldenstaedtii. Two mitochondrial lineages are present within this species: one is phylogenetically affiliated with A. persicus and A. naccarii, while the other clusters with A. baerii. These findings have a direct impact on molecular testing of commercial caviar and demonstrate the necessity of using large sample sizes when constructing forensic databases. Furthermore, the results affect current taxonomic designations for these species as well as hypotheses concerning their evolutionary origins.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1010141906100
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  • 8
    Electronic Resource
    Electronic Resource
    Sex ratio of leopards taken in trophy hunting: genetic data from Tanzania (2000)
    Springer
    Conservation genetics 1 (2000), S. 169-171 
    Details
    ISSN: 1572-9737
    Keywords: conservation ; Panthera pardus ; sport hunting ; tourism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The sex ratio of leopards, Panthera pardus, taken by trophyhunters in Tanzania is examined. We used sex specific molecularmarkers to analyze 77 samples collected from animals shot betweenthe years 1995–1998 and found that 28.6% were females, despitethe fact that only males are allowed on licenses and all skinswere tagged as males. The model used for quota setting assumesthat only males are shot, but the effect of this violation ofquotas is unknown. Off-take in Tanzania does not currently fillquotas, but when off-take approach maximum levels, compliancewith set quotas and regulations will be critical for sustainableharvest.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1026543308136
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  • 9
    Electronic Resource
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    Landscape change patterns in mountains, land use and environmental diversity, Mid-Norway 1960–1993 (2000)
    Springer
    Landscape ecology 15 (2000), S. 155-170 
    Details
    ISSN: 1572-9761
    Keywords: biodiversity ; conservation ; disturbance ; forest succession ; fragmentation ; grazing ; land use change ; mountain ; semi-natural grasslands ; sub-alpine ; sustainable agriculture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Norwegain mountains have had a central role in the subsistence agroecosystems by providing vast biological resources for humans and their livestock since 4000–3500 BP as indicated by paleoecological records. Later with the development of the summer farming system the use of the mountains was intensified. This long-term use of the mountains has shaped a montane cultural landscape by livestock grazing, mowing for hay, fuel collection and a variety of other uses. The result is a significant increase of the grassland areas at the expense of the forest. Those semi-natural grasslands and heathlands with specific biological diversity have until recently dominated the mountains but are today decreasing due to forest invasion – which in turn is a result of changes in human land use. The present paper focuses on changes in landscape pattern and differences in landscape development in two mountain valleys with summer farming activities, in Mid-Norway, over the period 1960s–1990s, and seeks to interpret the changes in relation to differential land use and environmental factors. This study contributes examples from human shaped ecosystems in mountains where the fragmentation of semi-natural habitats is addressed. A set of landscape pattern indices commonly used in landscape ecological studies is also used here, and their ecological relevance in the present context is dealt with. The implications of changed land use for biodiversity conservation in those mountains and the relationships to future sustainable agriculture is also briefly discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008173628016
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  • 10
    Electronic Resource
    Electronic Resource
    Integrated Biodiversity Management in Paddy Fields: Shift of Paradigm From IPM Toward IBM (2000)
    Springer
    Integrated pest management reviews 5 (2000), S. 175-183 
    Details
    ISSN: 1572-9745
    Keywords: paddy agroecosystem ; minor insects ; non-target insects ; conservation ; agroecology ; aquatic insects
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The insect fauna in paddy fields is composed of resident, migratory and aquatic species each corresponding to the continuous cropping of rice in the same field, harvesting rice as an annual crop, and originating from still water habitats in wetlands. Although IPM is becoming popular in the control of rice pests, those ‘minor’ insects and aquatic insects that have no direct economic impact on rice production have received little attention. Consequently, some of them are in danger of extinction requiring conservation. A new concept, ‘Integrated biodiversity management (IBM)’, is proposed under which IPM and conservation are reconciled and made compatible with each other. As an operational concept in agroecology, premises for implementing IBM are suggested.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1011315214598
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  • 11
    Electronic Resource
    Electronic Resource
    Gap analysis: concepts, methods, and recent results* (2000)
    Springer
    Landscape ecology 15 (2000), S. 5-20 
    Details
    ISSN: 1572-9761
    Keywords: biodiversity ; conservation ; large-area mapping ; gap analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Rapid progress is being made in the conceptual, technical, and organizational requirements for generating synoptic multi-scale views of the earth's surface and its biological content. Using the spatially comprehensive data that are now available, researchers, land managers, and land-use planners can, for the first time, quantitatively place landscape units – from general categories such as ‘Forests’ or ‘Cold-Deciduous Shrubland Formation’ to more categories such as ‘Picea glauca-Abies balsamea-Populus spp. Forest Alliance’ – in their large-area contexts. The National Gap Analysis Program (GAP) has developed the technical and organizational capabilities necessary for the regular production and analysis of such information. This paper provides a brief overview of concepts and methods as well as some recent results from the GAP projects. Clearly, new frameworks for biogeographic information and organizational cooperation are needed if we are to have any hope of documenting the full range of species occurrences and ecological processes in ways meaningful to their management. The GAP experience provides one model for achieving these new frameworks.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008184408300
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  • 12
    Electronic Resource
    Electronic Resource
    Population structure and genetic variability of pearl oyster Pinctada mazatlanica along Pacific coasts from Mexico to Panama (2000)
    Springer
    Conservation genetics 1 (2000), S. 299-308 
    Details
    ISSN: 1572-9737
    Keywords: conservation ; genetic variability ; isolation by distance ; mitochondrial DNA ; pearl oyster
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Populations of the Calafia pearl oyster Pinctada mazatlanica ofthe American Pacific coasts have been considered endangeredbecause of overfishing and/or alteration to coastal areas. Weassessed genetic variability and the pattern of populationstructure among 9 samples collected from Mexico to Panama, usingmtDNA RFLP analysis of two genes: 12S rRNA and subunit one ofCytochrome oxydase (COI). Haplotype diversity varied from 0.000to 0.856. The Panama population appeared to be monomorphic, whilethe other samples exhibited a level of haplotypic variabilitysimilar to those reported in the literature for the same kind ofanalysis on other bivalves species. A test for the impact ofdemographic history on genetic diversity was applied on thesequence data, and the results were congruent with a recentdecline of population sizes. Genetic differentiation was shown tofollow a scheme of isolation by distance, with low levels ofdifferentiation at the scales of ten to one hundred kilometres,whereas stronger and significant genetic structure was detectedat a larger scale. Three significantly distinct groups could thenbe defined, which correspond to Northern Mexico, Southern Mexico,and Panama.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1011575722481
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  • 13
    Electronic Resource
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    Effects of flooding duration on species richness, floristic composition and forest structure in river margin habitat in Amazonian blackwater floodplain forests: implications for future design of protected areas (2000)
    Springer
    Biodiversity and conservation 9 (2000), S. 1-14 
    Details
    ISSN: 1572-9710
    Keywords: Amazonia ; blackwater ; Brazil ; conservation ; community ordination ; floodplain forests ; forest structure ; species richness ; species distributions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Rivers in central Amazonia experience annual water-level fluctuations of up to 14 m, flooding vast areas of adjacent forest for periods ranging from a few to 270 days per year. At different sites, variation in the duration and type of flooding results in a mosaic of habitats that includes lakes, grasslands, forests, and streams. To study the effects of flood duration on plant species richness and floristic composition, two river margin sites were surveyed on the rivers Jaú and Tarumã-Mirim. Both areas are seasonally flooded by blackwaters, and plots were made at different topographic levels (lower, middle and upper slopes). All woody plants with DBH 〉 5cm were inventoried in five 10 × 40 m plots in each of the three topographic levels, which varied in length of flood duration and mean water level. Plant species richness did not vary significantly between topographic levels, but species composition varied substantially. At both study sites, the species composition exhibited distinctive distribution patterns with respect to the three topographic levels and river site. Differences in the distribution of dominant species in both sites probably relate to the ability of species to withstand seasonal flooding, although other edaphic factors associated with the topographic levels may also be important, especially for less-dominant, locally rare, and habitat generalist species. Species composition overlap among topographic levels at the two sites was highly variable ranging from 15% to 43%. Knowledge about the complex pattern of species composition and distributions between and among topographic levels and river sites is important for the preservation of the diverse flora of the blackwater forests and for the creation of future conservation management plans and design of protected areas in this ecosystem that will maintain the biodiversity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008989811637
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  • 14
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    Bird species richness in vegetation fences and in strips of residual rain forest vegetation at Los Tuxtlas, Mexico (2000)
    Springer
    Biodiversity and conservation 9 (2000), S. 1399-1416 
    Details
    ISSN: 1572-9710
    Keywords: bird diversity ; conservation ; corridors ; forest fragmentation ; Los Tuxtlas ; Mexico ; tropical rain forests
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Fragmentation of the lowland tropical rain forest has resulted in loss of animal and plant species and isolation of remaining populations that puts them at risk. At Los Tuxtlas, Mexico, lowland rain forests are particularly diverse in the avian fauna they contain and while most of the forests have been fragmented by human activity, many of the fragments still harbor diverse assemblages of bird species. In these landscapes, linear strips of residual rain forest vegetation along streams as well as linear strips of vegetation fences (live fences) crossing the pastures might provide some connectivity to bird populations existed in forest fragments. We investigated bird species richness and relative abundance in one 6-km long section of live fences (LF) bordering a dirt road and in two 6-km long sections of residual forest vegetation along a river (MR) and one permanent stream (BS). We used point count procedures which resulted in the count of 2984 birds representing 133 species. At the LF site we detected 74% of the species, 72% at the BS site and 57% at the MR site. Only 38% of the species were common among sites. Neotropical migratory birds accounted for 34–41% of the species counted at all sites. While edge and open habitat birds accounted for 6–10% of the species and for 50% of the records at the three vegetation strips, about 90% of the species were forest birds. Distance to forest fragments and degree of disturbance of the vegetation seemed to negatively influence bird species presence at the BS and MR strips. Rarefaction analysis indicated that the LF strip was richer in species than the other two sites, but the occurrence of the three vegetation strips in the landscape seem to favor the presence of many more species. We discuss the value of these vegetation strips to birds as stepping stones in the fragmented landscape.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008935016046
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  • 15
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    Comparison of genetic population structure of the large blue butterflies Maculinea nausithous and M. teleius (2000)
    Springer
    Biodiversity and conservation 9 (2000), S. 419-432 
    Details
    ISSN: 1572-9710
    Keywords: allozymes ; conservation ; Lycaenidae ; Maculinea ; metapopulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We investigated the genetic population structure of two rare myrmecophilous lycaenid butterflies, Maculinea nausithous and M. teleius, which often live sympatrically and have similar biology. In Europe, both species occur in highly fragmented populations and are vulnerable to local extinction. The proportion of variable allozyme loci, average heterozygosity and genetic differentiation among populations was higher in M. nausithous than in sympatrically living M. teleius populations. We hypothesise that the differences in heterozygosity are mainly due to the known higher efficiency of typical host ant nests in rearing M. nausithous pupae compared to M. teleius pupae. This implies a larger probability of larval survival in M. nausithous, which buffers populations against environmental and demographic stochasticity. In contrast, the lower carrying capacity of ant nests in rearing M. teleius pupae requires higher nest-densities and makes M. teleius populations more prone to losing genetic variation through drift if this condition is not fulfilled. The single investigated Russian population of M. teleius showed much higher levels of heterozygosity than any of the Polish populations, suggesting a more viable and still intact metapopulation structure.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008970232079
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  • 16
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    Mammal fauna of the Muni-Pomadze Ramsar site, Ghana (2000)
    Springer
    Biodiversity and conservation 9 (2000), S. 541-560 
    Details
    ISSN: 1572-9710
    Keywords: biodiversity ; bushmeat ; conservation ; duikers ; hunting ; insectivores ; mammals ; monitoring ; rodents
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A survey of the terrestrial mammal fauna of Muni-Pomadze Ramsar site along the Ghana coastline recorded 13 species, with 9 additional species reported by local villagers. Small mammal communities are most diverse in the grassland-thicket habitats surrounding the lagoons. Less diverse communities were present in and around managed Ecalyptus plantations or near villages. Duikers, antelope, bushbuck (Tragelaphus scriptus), and cane rats (Thryonomys swinderianus) are hunted (or trapped) for bushmeat. Bushbuck, in particular, play an important role in certain cultural ceremonies at Muni-Pomadze. Conservation efforts at these localities should focus on the management of bushmeat species and the establishment of a long-term monitoring program using small mammal communities as bioindicators of the health of the terrestrial habitats bordering Muni Lagoon.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008964000018
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  • 17
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    Postmodernism and African conservation science (2000)
    Springer
    Biodiversity and conservation 9 (2000), S. 559-577 
    Details
    ISSN: 1572-9710
    Keywords: Africa ; conservation ; deconstruction ; ecology ; knowledge ; science ; sociology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In Africa, the movement away from traditional protectionist conservation to community-based approaches is partially related to postmodernist influences. Proposed transfrontier conservation areas will incorporate local communities, and a clearer understanding of the limitations of community-based conservation is thus needed. The sustainability of community-based conservation projects is questioned on economic and other grounds, and many African countries lack the prerequisites (ecological, demographic and sociological) for successful programmes. The romanticisation of pre-colonial societies gives undue weight to traditional systems of resource management, and we challenge the postmodernist notion that traditional peoples practised sustainable harvesting of natural resources. It is suggested that this will occur only under unlikely conditions of low human population density, lack of access to modern technology, and limited exposure to consumerism. In agriculture, postmodernists interpret the overstocking of livestock as a rational socioeconomic response, thus giving the practice unjustified legitimacy. The allied proposition that peasants enjoy a rich diversity of farming practices is largely unfounded, at least in some parts of Africa. We conclude that postmodernist thinking has had a significant negative impact on conservation science in Africa, largely by marginalising the central issue of human population pressure. Towards more effective African conservation, we suggest roles for the ecologist, for the social scientist, and for the donor community.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1008972612012
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  • 18
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    Distribution and Conservation of Slender Lorises (Loris tardigradus lydekkerianus) in Southern Andhra Pradesh, South India (2000)
    Springer
    International journal of primatology 21 (2000), S. 721-730 
    Details
    ISSN: 1573-8604
    Keywords: Loris tardigradus lydekkerianus ; Andhra Pradesh ; South India ; conservation ; Eastern Ghats
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Slender lorises live in forests of southern India and Sri Lanka. Little is known about their distribution patterns and relative densities. We report the results of a survey conducted in 6 forest divisions in the southern parts of the state of Andhra Pradesh, South India. Relatively high densities of lorises occurred in mixed deciduous forests and in adjoining farm lands interspersed with trees. Three distinct populations inhabit the study area. We recommend conservation measures for Loris tardigradus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1005573506489
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  • 19
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    Primate Community Dynamics in Old Growth Forest over 23.5 Years at Ngogo, Kibale National Park, Uganda: Implications for Conservation and Census Methods (2000)
    Springer
    International journal of primatology 21 (2000), S. 269-286 
    Details
    ISSN: 1573-8604
    Keywords: conservation ; censuses ; population dynamics ; primates
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Few data exist regarding long-term changes in primate populations in old-growth, tropical forests. In the absence of this information, it is unclear how to assess population trends efficiently and economically. We addressed these problems by conducting line-transect censuses 23.5 years apart at the Ngogo study area in Kibale National Park, Uganda. We conducted additional censuses over short time intervals to determine the degree to which the temporal distribution of censuses affected estimates of primate numbers. Results indicate that two species, blue monkeys and red colobus, may have experienced significant reductions over the past 23.5 years at Ngogo. In contrast, five other species, baboons, black-and-white colobus, chimpanzees, mangabeys, and red-tailed guenons, have not changed in relative abundance. Additional findings indicate that different observers may vary significantly in their estimates of sighting distances of animals during censuses, thus rendering the use of measures of absolute densities problematic. Moreover, censuses conducted over short periods produce biased estimates of primate numbers. These results provide guidelines for the use of line-transect censuses and underscore the importance of protecting large blocks of forests for primate conservation.
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    URL: http://dx.doi.org/10.1023/A:1005477504728
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    Coping with Forest Fragmentation: The Primates of Kibale National Park, Uganda (2000)
    Springer
    International journal of primatology 21 (2000), S. 587-611 
    Details
    ISSN: 1573-8604
    Keywords: forest fragmentation ; forest patches ; primate communities ; Colobus guereza ; Kibale National Park ; conservation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A goal of conservation biology is to determine which types of species are most susceptible to habitat disturbance and which types of disturbed habitats can support particular species. We studied 20 forest fragments outside of Kibale National Park, Uganda, to address this question. At each patch, we determined the presence of primate species, tree species composition, patch size, and distance to nearest patch. We collected demographic, behavioral, and dietary data for Abyssinian black-and-white colobus (Colobus guereza). Black-and-white colobus and red-tailed guenons (Cercopithecus ascanius) were in almost all fragments; Pennant's red colobus (Procolobus pennantii) and chimpanzees (Pan troglodytes) were in some fragments; and blue monkeys (Cercopithecus mitis) and gray-cheeked mangabeys (Lophocebus albigena) were absent from all fragments. No species characteristics—home range, body size, group size, or degree of frugivory—predicted the ability of species to live in patches. No characteristics of patches—area, distance to the nearest patch, distance to Kibale, or number of food trees present—predicted the presence of a particular species in a patch, but distance to Kibale may have influenced presence of red colobus. Black-and-white colobus group size was significantly smaller in the forest patches than in the continuous forest of Kibale. For a group of black-and-white colobus in one patch, food plant species and home range size were very different from those of a group within Kibale. However, their activity budget and plant parts eaten were quite similar to those of the Kibale group. The lack of strong predictive variables as well as differences between other studies of fragmentation and ours caution against making generalizations about primate responses to fragmentation.
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    URL: http://dx.doi.org/10.1023/A:1005509119693
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    Vegetative propagation of three mangrove tree species by cuttings and air layering (2000)
    Springer
    Wetlands ecology and management 8 (2000), S. 281-286 
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    ISSN: 1572-9834
    Keywords: conservation ; excoecaria ; heritiera ; intsia ; mangroves
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Large scale propagation of three mangrove species, Excoecaria agallocha, Heritiera fomesand Intsia bijugausing cuttings and air layering was attempted. The effect of auxins and season on rooting potential of these mangrove species was recorded. Maximum rooting was recorded when the cuttings and air layers were treated with IBA alone up to 2500 ppm in all the three species. October was found to be best followed by January for the plantation of cuttings and initiation of air layers. All the plants were hardened and field transferred into the mangrove forests of Pichavaram, Tamilnadu, India.
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    URL: http://dx.doi.org/10.1023/A:1008481222718
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    The use of allozymes at three life stages influences genetic variation analysis and clustering in Cicer arietinum germplasm collections (2000)
    Springer
    Euphytica 112 (2000), S. 109-115 
    Details
    ISSN: 1573-5060
    Keywords: allozymes ; Bangladesh ; chickpea ; Cicer arietinum ; conservation ; genetics ; germplasm ; populations ; variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Levels of genetic variation using 6 enzyme systems for a total of 11 interpretable loci were examined in chickpea (Cicer arietinum) originating from 9locations in Bangladesh. The measurement of genetic variation at enzyme loci was carried out on the seed embryo, on the early leaves of seedlings and on the mature leaves at the vegetative stage. A total of 592individuals, including 240 seeds, 200 seedlings and152 mature leaves were investigated. Using electrophoretic data, chickpea was found to express higher percentages of polymorphic loci at the seed stage (36–64%) than at seedling (22–56%) or the vegetative stage (11–44%). The proportion of mean number of alleles and the average mean observed heterozygosity also were higher at the seed stage when compared to the seedling and vegetative stages. Unique alleles were absent, and only differences infrequencies could be noticed. Positive values of the fixation index were noted for pgm-1 and 6pg-1 for all stages and in both mnr loci for the seed embryo's. A trend towards lower genetic distances of all possible pairs of populations could be observed when comparing those of seed embryo's with seedlings or mature leaves. This trend was even more pronounced when pooling the data of 9 populations into their 3regions. Slight differences in genetic distances caused a separative clustering of population 3 at seed embryo, of population 2 at seedling and of population5 at vegetative stages. It is suggested that careful examination of enzyme polymorphisms at different developmental stages is a prerequisite before drawing conclusions on the genetic distance between germplasm collections from different origins since small differences in the data entry for clustering results in ties that may affect tree topologies.
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    URL: http://dx.doi.org/10.1023/A:1003851113039
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    Micropropagation of the critically endangered Western Australian species, Symonanthus bancroftii (F. Muell.) L. Haegi (Solanaceae) (2000)
    Springer
    Plant cell, tissue and organ culture 63 (2000), S. 23-29 
    Details
    ISSN: 1573-5044
    Keywords: acclimatisation ; antioxidants ; conservation ; potassium citrate ; tissue browning ; tissue culture ; triazoles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A micropropagation protocol was developed for the conservation of the critically endangered Western Australian shrub,Symonanthus bancroftii. It was necessary to screen antioxidant treatments to prevent the occurrence of lethal browning of explants upon excision. Potassium citrate and citric acid (0.1% w/v in a 4:1 ratio) prevented oxidative browning and was superior to the untreated control or other antioxidant treatments tested. Half strength Murashige and Skoog (MS) medium containing 0.5 μM kinetin and 0.25 μM benzyladenine produced three-fold multiplication compared to 1.75×, 1.5×, 1.8× and 1× multiplication for 2.5 μM kinetin + 0.25 μM benzyladenine, 0.5 μM kinetin + 5 μM gibberellic acid, 1 μM kinetin + 3 μM gibberellic acid and half strength MS with no plant growth regulators, over 4 weeks. Root production was achieved with indole-3-butyric acid (IBA) and α-naphthaleneacetic acid (NAA) at 0.5/0.5 μM (31% rooting) and 1.0/1.0 μM (36% rooting), after four weeks. Paclobutrazol (PBZ) at 0, 3.4 (1 mg 1−1), 10.2 (3 mg 1−1), or 17 μM (5 mg 1−1) improved tolerance to desiccation after transfer ofin vitro rooted shoots to soil. PBZ at 10.2 μM increased survival to 90% compared to 50% for those plantlets not treated with PBZ. The acclimatisation period from the glasshouse to the shadehouse was 1 week for plantlets treated with PBZ compared to 4 weeks for plantlets without any PBZ. PBZ at 3.4 μM increased the number of roots per shoot compared to untreated controls.
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    URL: http://dx.doi.org/10.1023/A:1006457624282
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    Distribution and status of the medicinal leech (Hirudo medicinalis L.) in Turkey (2000)
    Springer
    Hydrobiologia 441 (2000), S. 37-44 
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    ISSN: 1573-5117
    Keywords: distribution ; status ; population assessment ; zoogeography ; conservation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A survey of all the major potential habitats in western Turkey showed that medicinal leeches, Hirudo medicinalis L., are widely distributed over the country and are not rare. They occur in practically all suitable habitats and the only region where they were found to be absent is that of the large river deltas in the south of the country (Çukurova deltas, Göksu delta). There may be zoogeographic reasons for this (Taurus mountains barrier). The application of a semi-quantitative survey method using collecting efficiency (number of leeches collected per hour by a single person) allowed a rapid assessment to be made of its status in a large number of wetlands. Leech density varied considerably from wetland to wetland, and the results enabled a ranking of the Turkish wetlands to be made according to their importance for medicinal leeches. Taking both the leech density and the size of leech habitats into account, the largest populations were identified on the Black Sea coast (Kizilirmak delta, Yeşilirmak delta and Karagöl Marshes near Sinop) and in inner and south-west Anatolia (Eber Gölü, Karamik and Sultan Marshes). Commercial exploitation for the pharmaceutical industry and for other purposes takes place at only a few places and does not appear to affect the population seriously. However, many populations are threatened by the draining of their habitats.
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    URL: http://dx.doi.org/10.1023/A:1017555322002
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    Genetics of marine bioinvasions (2000)
    Springer
    Hydrobiologia 420 (2000), S. 63-71 
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    ISSN: 1573-5117
    Keywords: ballast water ; conservation ; invasive species ; population structure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Anthropogenic biological introductions have captured the attention of marine scientists and resource managers in recent years. Human-mediated marine bioinvasions are presently acknowledged as often ecologically and financially devastating events. Despite recent increases in scientific interest and financial resources devoted to nonindigenous nuisance species globally, fundamental questions pertaining to taxonomic identity, geographic source, introduction vector and invasive population dynamics frequently remain unanswered. Ecological surveys based on photometric and observational methods are unable to identify cryptogenic taxa, source populations, multiple introductions, or genetic diversity. The twofold goal of this paper is to discuss the application of molecular genetic techniques to fundamental ecological questions pertaining to bioinvasions and to demonstrate the utility of DNA technology in providing data useful in the development of predictive models for marine bioinvasion science.
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    URL: http://dx.doi.org/10.1023/A:1003929519809
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    Temporal Genetic Variation in a Population of Aphanius fasciatus (Cyprinodontidae) from a Brackish-water Habitat at Elba Island (Italy) (2000)
    Springer
    Environmental biology of fishes 57 (2000), S. 107-112 
    Details
    ISSN: 1573-5133
    Keywords: conservation ; allozymes ; generations ; genetic distance ; genetic drift ; selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Allozyme electrophoresis was used to assess temporal genetic variation in three successive generations of the Mediterranean killifish, Aphanius fasciatus. Samplings were carried out in 1995, 1996 and 1997 in a brackish-water habitat at Elba Island, Italy and a total of 212 specimens were collected. The five loci for which polymorphism has been detected in a previous study were assayed. Mean expected heterozygosity values [H=0.397 (SE 0.077), H=0.336 (SE 0.092) and H=0.313 (SE 0.092) in 1995, 1996 and 1997, respectively] were not significantly different by ANOVA test. Deviations from Hardy–Weinberg equilibrium were minimal, with only one out of the 15 probability tests showing a significant departure from the equilibrium; whereas genotypic linkage disequilibrium was not detected. Values of Nei's genetic distance were lower than 0.04. Temporal genetic variation in the A. fasciatus population at Elba Island was observed, with F-statistics indicating significant genetic divergence among samples (θ=0.035, SE 0.027, p〈0.001). Genetic drift acting on two loci (GPD-1 * and LDH-3 *) is presumably the main force determining the temporal genetic heterogeneity observed; however, the occurrence of selection on individual loci and/or sampling error cannot be excluded. The observed allelic variation among generations in a single population of A. fasciatus is much less than levels observed among geographically discrete samples in previous studies.
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    URL: http://dx.doi.org/10.1023/A:1007604831242
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    Predicting Population Recovery Rates for Endangered Western Atlantic Sawfishes Using Demographic Analysis (2000)
    Springer
    Environmental biology of fishes 58 (2000), S. 371-377 
    Details
    ISSN: 1573-5133
    Keywords: largetooth sawfish ; Pristis perotteti ; smalltooth sawfish ; Pristis pectinata ; life tables ; conservation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sawfish are a group of endangered elasmobranchs that were common in tropical inshore, estuarine and freshwaters. The demography of two species of sawfish that occur in the western Atlantic – Pristis pectinata and P. perotteti – was investigated using age-structured life tables. Life history parameters for use in the life tables were obtained from published data. Five scenarios were tested for P. pectinata to incorporate uncertainties about life history data. Values of intrinsic rate of increase ranged from 0.08 to 0.13 yr−1, and population doubling times from 5.4 to 8.5 yrs. Eight scenarios were tested for P. perotteti. The most likely range for the intrinsic rate of increase was 0.05–0.07 yr−1, with population doubling times of 10.3–13.5 yrs. Four scenarios investigating the sensitivity to methods of estimating natural mortality produced similar results. The demographic results were sensitive to changes in reproductive periodicity and natural mortality. The results indicate that if effective conservation plans can be implemented for sawfish and sawfish habitats, recovery to levels where there is little risk of extinction will take at least several decades.
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    URL: http://dx.doi.org/10.1023/A:1007675111597
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    Decline of Native Freshwater Fishes in a Mediterranean Watershed on the Iberian Peninsula: A Quantitative Assessment (2000)
    Springer
    Environmental biology of fishes 59 (2000), S. 11-19 
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    ISSN: 1573-5133
    Keywords: conservation ; distribution ; environmental degradation ; endangered species ; introduced species
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We examined changes in the distribution of 9 native and 18 introduced freshwater fishes in the south-eastern Pyrenees watershed, Iberian Peninsula, using data from 1996, 1984–1988 and historical information. This region suffers many modifications to its freshwater ecosystems that are linked to human activity in the Mediterranean regions. Fish communities, stream physical habitat and environmental degradation were assessed at 168 sites from 11 basins in 1996. Seven native species (78%) showed decline from previous data, one of which became extirpated in the first half of the 20th century. On the other hand, introduced species are expanding. As a consequence, intact native communities are increasingly rare, declining from presence in 22% of river courses in 1984–1988 to 15% in 1996. The most typical community type is a mixture of native and introduced species occupying 30% of river courses. Stream degradation seems to be the main cause of this process because fish communities differed between degraded streams and streams suffering less impact. A principal component analysis showed that water pollution and modifications to the habitat were the two anthropogenic factors that accounted for most changes in the fish community integrity. Habitat alteration, primarily through construction of dams and water diversions, has fragmented habitats and isolated native fish communities in headwater streams. Current protection measures do not offer effective conservation of threatened species and communities. A global conservation and restoration programme from an ecosystem-based approach is essential to reverse the trend affecting native freshwater fishes in this Mediterranean region.
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    URL: http://dx.doi.org/10.1023/A:1007618517557
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    Aquarium Maintenance of Reef Octocorals Raised from Field Collected Larvae (2000)
    Springer
    Aquarium sciences and conservation 2 (2000), S. 227-236 
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    ISSN: 1573-1448
    Keywords: aquarium ; conservation ; Eilat ; planula larvae ; soft corals
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The development of standardized and sustainable aquaculture techniques for the cultivation of marine organisms offers many advantages: it would help reduce the quantity of living material harvested from coral reefs; it could be used as an efficient means of rehabilitating impoverished reef ecosystems (e.g. by reseeding depleted natural stocks of corals); it would provide an educational and research tool for the intensive study of marine animal biology under controlled laboratory conditions. Here, we present the results of aquarium maintenance of 3 Red Sea soft coral species (Clavularia hamra, Nephthea sp., Litophyton arboreum) raised from field collected larvae. Planulae settlement was enhanced when dead coral fragments and stones freshly collected from the sea were added to the settlement dishes. Young colonies (n=106, 258, 60; respectively) were monitored for 307, 475 and 207 days, respectively. The survival rate at the end of the observations ranged between 17% and 30%. Growth rates of colonies differed and showed species-specific variations. The most successful growth was recorded in Nephthea where the average colony size reached 324.5 polyps. Current aquaculture techniques for alcyonarian corals not only reveal that these organisms can thrive in aquaria but also provide significantly improved yields of colonies, as compared with yields under field conditions. Our studies have shown that various steps involved in the cultivation of young corals in captivity may need to be specifically tailored to suit the coral species in question.
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    URL: http://dx.doi.org/10.1023/A:1009627313037
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    Preliminary success in closing the life cycle of exploited seahorse species, Hippocampus spp., in captivity (2000)
    Springer
    Aquarium sciences and conservation 2 (2000), S. 179-196 
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    ISSN: 1573-1448
    Keywords: seahorses ; Hippocampus ; conservation ; trade ; husbandry ; aquaculture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The trade in seahorses for aquarium fishes is contributing to the depletion of many wild populations of these animals. Many seahorses are sold to replace those that have died in captivity as a result of husbandry problems. It can be particularly difficult to rear the young seahorses, because of their need for varied live food and their vulnerability to disease. We here report a pilot study on rearing broods from males of three species (H. fuscus, H. barbouri, and H. kuda) that had mated in the wild and gave birth in captivity. The new-born seahorses were fed an initial diet of enriched Artemia until 7 days, after which copepods were added to the diet. From 5 weeks, frozen mysids were gradually phased in to replace both other food items. Scrupulous hygiene was maintained. We achieved 100% survival of the partial broods we reared for all three species and achieved life cycle closure in two of these during the experimental period. Of the three species, H. kuda grew to be largest and longest, and H. barbouri grew least. However, H. kuda were the slowest to mature and reproduce while H. fuscus (intermediate in growth) were the fastest. Techniques used in this work should be more generally applicable, both for aquarium husbandry and for small-scale aquaculture to help provide alternative incomes for small-scale fishers who are otherwise dependent on catching wild seahorses.
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    URL: http://dx.doi.org/10.1023/A:1009629130932
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    Ex situ Culture of Colonial Marine Ornamental Invertebrates: Concepts for Domestication (2000)
    Springer
    Aquarium sciences and conservation 2 (2000), S. 237-250 
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    ISSN: 1573-1448
    Keywords: aquarium industry ; conservation ; coral reef ; domestication ; marine ornamental invertebrates
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The worldwide market for ornamental saltwater invertebrates supplies the needs of millions of aquarium hobbyists, as well as for public exhibition (zoos, aquaria), universities, and research institutions. The large-scale continuous collection of marine organisms is responsible, in many places, for the destruction of habitats, including coral reefs. The perceived expansion of the animal trade further threatens these fragile habitats. In the present paper, several concepts for the domestication of marine ornamental invertebrates (mainly colonial species) are discussed, offering an alternative commercial approach. The major rationale is based on future ex situ propagation, not field collections; a strategy aimed to circumvent the need for wild-harvested animals. This strategy is based on: (1) collection, settlement and metamorphosis of large numbers of larvae from marine organisms or of naturally shed germ cells under aquarium conditions, where survivorship exceeds several orders of magnitude than that in nature; (2) fragmentation of very small pieces (such as the size of a single polyp in colonial corals or blood vessel ampullae in tunicates) for the production of new colonies; (3) the development of replicates and inbred-lines from chosen ornamental species; (4) the use of cryopreservation of larvae and germ cells which will support the supply of material year-round; (5) several concepts for husbandry methods. Some benefits and deficiencies associated with the strategy for ex situ cultures are discussed, revealing its importance to the future of the trade.
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    URL: http://dx.doi.org/10.1023/A:1009664907098
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    Geographical perspectives on Polish tourism (2000)
    Springer
    GeoJournal 50 (2000), S. 173-179 
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    ISSN: 1572-9893
    Keywords: conservation ; employment ; leisure ; Lower Silesia ; Poland ; recreation ; regulation ; tourism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geography
    Notes: Abstract Like other sectors of the Polish economy, the tourist industry is going through a radical restructuring process which began at the start of the 1990s and reached a new stage with the 1998 Tourist Services Act. The business is now firmly market-oriented and standards are being set in line with EU norms. Tourism is of great importance for Poland because, through the promotion of attractive natural and cultural landscapes of international importance, it plays a central role in the search for a more sustainable economy and it also enhances the potential for development in many of the country's poorer regions. Employment in the industry is increasing both relatively and absolutely. This paper examines the opportunities with particular reference to Lower Silesia where the tourism potential offers an important approach to the solution of the region's economic and social problems. Particular reference is made to the refurbishment of historic buildings for tourist use and the significance of the restoration of Krobielowice Palace near Wroclaw is fully evaluated.
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    Shifting Cultivation and Conservation of Biological Diversity in Tripura, Northeast India (2000)
    Springer
    Human ecology 28 (2000), S. 605-629 
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    ISSN: 1572-9915
    Keywords: shifting cultivation ; biological diversity ; conservation ; Northeast India ; Tripura
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Ethnic Sciences
    Notes: Abstract Shifting cultivation (jhooming) has been identified as one of the main human impacts influencing biodiversity in Tripura, Northeast India. Over the last few years a new class of shifting cultivators has emerged that has adopted non-traditional forms of jhooming, which have been responsible for the loss of biological diversity in the state. This paper describes the successes achieved by the state government in providing the jhumias (tribes practicing jhooming) with various non-jhooming options. Recommendations include the need for short and long term control measures, improvement of existing jhooming methods, and integration of traditional knowledge with new practices.
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    URL: http://dx.doi.org/10.1023/A:1026491831856
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    Romania's Apuseni Mountains: Safeguarding a cultural heritage (2000)
    Springer
    GeoJournal 50 (2000), S. 285-304 
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    ISSN: 1572-9893
    Keywords: accessibility ; conservation ; cultural landscape ; demography ; light industry ; rural planning ; rural settlement ; tourism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geography
    Notes: Abstract The Apuseni Mountains are unusual on account of the extreme fragmentation of the settlement pattern particularly in the upper Aries valley. Not only is hamlet settlement very much the rule, but these small units of settlement may be quite widely separated from each other by distance and altitude. Such networks have evolved through clearance of the high surfaces which cannot be efficiently farmed from the valleys in which modern infrastructure has been concentrated over the last century. Important questions therefore arise with regard to the survival capacity of communities which are critical elements in a cultural landscape that constitutes a valuable resource for both Romania and Europe. In other parts of the region dispersal is not so extreme, but there are still depopulation tendencies which are potentially damaging for the cultural landscape. The paper examines the lifestyles associated with outlying settlements and the extent to which centralising tendencies have been resisted in recent times. In the context of the present transition it considers the actions being considered to safeguard fragile mountain communities in a vulnerable area exposed to growing commercial penetration associated with cross-border cooperation on the Hungarian- Romanian frontier.
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    URL: http://dx.doi.org/10.1023/A:1007170018729
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    Environmental economics of the Khangchendzonga National Park in the Sikkim Himalaya, India (2000)
    Springer
    GeoJournal 50 (2000), S. 329-337 
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    ISSN: 1572-9893
    Keywords: contingent valuation ; bidding game ; biodiversity ; protected areas ; conservation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geography
    Notes: Abstract A contingent valuation survey was conducted involving local community members, domestic and foreign visitors to estimate the environmental economics of the Khangchendzonga National Park and to elicit their willingness-to-pay (WTP) for its maintenance and conservation. Using a random survey, the average WTP was US$ 8.84 for foreign visitors per visit, followed by US$ 6.20 per household per year by local community members and US$ 1.91 per domestic visitor per visit for improvement in environmental conservation. The WTP was strongly influenced by age, education and income. The present study demonstrated that the contingent valuation method (CVM) is a promising approach, however it lacks inclusion of non-monetary contributions. The WTP for environmental management by the local communities was mostly in kind or time for services. The CVM can be a useful tool for decision-makers regarding investment and policy purposes for management of biodiversity hot spots and protected areas in developing countries.
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    URL: http://dx.doi.org/10.1023/A:1011032813319
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    Policies for rural Eastern Europe in transition: The case of Slovakia (2000)
    Springer
    GeoJournal 50 (2000), S. 235-247 
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    ISSN: 1572-9893
    Keywords: conservation ; cross-border cooperation ; land use ; regional development ; restructuring ; rural development ; rural policies ; small and medium sized enterprises
    Source: Springer Online Journal Archives 1860-2000
    Topics: Geography
    Notes: Abstract After losing much of their demographic vitality under communism, rural areas of Eastern Europe are now handicapped by greatly reduced commuting opportunities and by a lack of foreign investment in the countryside. Although restitution and privatisation has placed much of the land under the control of private farmers, there is a need to modernise agriculture and rural infrastructure generally and also to increase the scope for pluriactivity. At the same time, functional links with the towns must be restored through a growth of employment linked with the central place system. Despite the importance of private enterprise under the conditions of a market economy, the government must take a lead and this paper deals with initiatives taken in rural Slovakia to mobilise the human resources of the countryside. The paper is heavily oriented towards research by the geographers at Nitra who have been much involved with the transformation of their local area. It is evident that while there has been no shortage of ideas, progress has been limited on account of low investment and the tensions associated with a series of three Meciar governments which have dominated Slovak politics through the 1990s.
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    URL: http://dx.doi.org/10.1023/A:1007149431025
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    In vitro storage of Eucalyptus grandis germplasm under minimal growth conditions (2000)
    Springer
    Plant cell, tissue and organ culture 61 (2000), S. 161-164 
    Details
    ISSN: 1573-5044
    Keywords: alginate ; axillary buds ; conservation ; E. grandis ; forestry ; shoot cultures
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Slow growth-storage, for up to 10 months, has been achieved for Eucalyptus grandis shoot cultures by either the addition of 10 mg l−1 abscisic acid to the growth medium or by the halving of nutrient supply (half MS) and removal of exogenous plant growth regulators. Reduction of light intensity or the addition of mannitol to the media were less effective in reducing growth rate. Isolated in vitro axillary buds encapsulated in calcium alginate and stored under low temperature and low light intensities survived for up to 3 months without loss in viability. Storage of such encapsulated fresh axillary buds at higher temperature resulted in a loss in viability. These methods have immediate applications to forestry breeding and clonal programs.
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    URL: http://dx.doi.org/10.1023/A:1006447506869
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    The development of integrated methods for assessing river conservation value (2000)
    Springer
    Hydrobiologia 422-423 (2000), S. 413-428 
    Details
    ISSN: 1573-5117
    Keywords: rivers ; conservation ; integrated ; evaluation ; SERCON
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In recent years the conceptual barriers between river `management' and river `conservation' have begun to erode. As the environmental aspirations of water managers and conservationists broaden, there is a need for integrated methods of conservation assessment which can be of value to both groups. This paper considers the requirements inherent in such methods, and explores some of the problems in their design, using for illustration the development of SERCON (System for Evaluating Rivers for Conservation) in the UK. The design of integrated assessment methods, including the selection of attributes for evaluation, must be closely related to the objectives of any conservation activity. For example, selecting a representative series of rivers for statutory protection may require more focused assessment techniques than those needed for setting river conservation management objectives. In the European Union, the Habitats Directive provides a good example of the former approach, in which specific river types and species listed in the Directive must be evaluated for selecting Special Areas of Conservation. In contrast, the forthcoming Water Framework Directive will need to rely on a more comprehensive method of integrated assessment to ensure that `good ecological quality' can be reliably defined, monitored and maintained. Attempts at developing integrated conservation assessment methods need to address six main issues: (i) differences in perception of conservation `value'; (ii) defining conservation criteria (such as `naturalness'), many of which are to an extent subjective; (iii) establishing weighting systems to differentiate important from less important conservation features; (iv) ensuring that methods are rigorous and consistent; (v) the need for extensive datasets to allow comprehensive assessment; and (vi) providing clear guidance on interpreting the outputs. This paper illustrates these problems using experience gained in the UK. It concludes that the inevitable search for pragmatism should not detract from the need for an inclusive approach to river conservation assessment.
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    URL: http://dx.doi.org/10.1023/A:1017054908152
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    Ecology of the River Dolphin (Platanista gangetica) in the Upper Brahmaputra (2000)
    Springer
    Hydrobiologia 430 (2000), S. 97-111 
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    ISSN: 1573-5117
    Keywords: Brahmaputra river ; hydrology ; aquafauna ; fishes ; river dolphin ; conservation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract There are nearly 200 species of aquatic vertebrates, almost exclusively fishes, in the Brahmaputra River System. This faunal composition includes not only a wide variety of food-fishes, but also about 50 varieties of aquarium fishes. The most spectacular animal in the Brahmaputra is undoubtedly the river dolphin, Platanista gangetica. The population of many species, particularly of the dolphin, is in steady decline. River dolphins, mostly juveniles, often entangle themselves in gill and drift nets, while feeding on trapped fishes. Proper implementation of Indian Fisheries Act, especially a total ban on destructive nets and on killing of brooders and juveniles by explosives and poisoning should be strictly implemented. Poor communication facilities in remote riverbank areas and lack of funds are two important reasons for the slow progress of the conservation of river dolphin and this endangered species is now on the verge of extinction. The anthropogenic activities and their impact on the habitat of river dolphin are discussed.
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    The Reintroduction and Reinterpretation of the Wild (2000)
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    Journal of agricultural and environmental ethics 13 (2000), S. 144-165 
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    ISSN: 1573-322X
    Keywords: Wild ; domestic ; animal ; conservation ; farming ; hunting ; tourism ; France
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Philosophy
    Notes: Abstract This paper is concerned with changing social representations of the ``wild,'' in particular wild animals. We argue that within a contemporary Western context the old agricultural perception of wild animals as adversarial and as a threat to domestication, is being replaced by an essentially urban fascination with certain emblematic wild animals, who are seen to embody symbols of naturalness and freedom. On closer examination that carefully mediatized ``naturalness'' may be but another form of domestication. After an historical overview of the human-animal, domestic-wild construction, an anthropological approach is used to interpret the social representation of wild animals held by different social actors – farmers, hunters, and tourists – within the context of an inhabited National Park, that of the Cévennes in south east France. Within the Park, the domestic and the wild, along with agriculture, hunting, conservation, re-introduced wild animals, and tourists co-habit. It is argued that changes in the representation of ``wildness'' may well be an important indicator ofchanges in the social representation of nature.
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    URL: http://dx.doi.org/10.1023/A:1009593919595
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    Biology and conservation of sturgeon and paddlefish (2000)
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    Reviews in fish biology and fisheries 10 (2000), S. 355-392 
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    ISSN: 1573-5184
    Keywords: Acipenseriformes ; conservation ; paddlefish ; phylogeny ; sturgeon ; threatened status
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The Acipenseriformes (sturgeon and paddlefish)live in the Northern Hemisphere; half of thesespecies live in Europe, mostly in thePonto-Caspian region, one third in NorthAmerica, and the rest in East Asia and Siberia.They reproduce in freshwater and most of themmigrate to the sea, either living in brackishwater (Caspian, Azov, Black and Baltic Seas) orin full seawater on the oceanic continentalshelf. Most species feed on benthic organisms.Puberty usually occurs late in life (5–30 yearsof age) and adult males and females do notspawn on an annual basis. Adults continue togrow and some species such as the beluga (Huso huso) have reached 100 years of age andmore than 1,000 kg weight. Stocks of sturgeonsare dramatically decreasing, particularly inEurasia; the world sturgeon catch was nearly28,000 t in 1982 and less than 2,000 t by 1999.This decline resulted from overfishing andenvironmental degradation such as: accumulationof pollutants in sediments, damming of rivers,and restricting water flows, which becomeunfavorable to migration and reproduction.Several protective measures have beeninstituted; for example, fishing regulation,habitat restoration, juvenile stocking, and theCITES listing of all sturgeon productsincluding caviar. In addition, sturgeon farmingpresently yields more than 2,000 t per year(equivalent to wild sturgeon landings) andabout 15 t of caviar. Hopefully, thisartificial production will contribute to areduction of fishing pressure and lead to therehabilitation of wild stocks.
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    The use of specimen-label databases for conservation purposes: an example using Mexican Papilionid and Pierid butterflies (2000)
    Springer
    Biodiversity and conservation 9 (2000), S. 1441-1466 
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    ISSN: 1572-9710
    Keywords: butterflies ; conservation ; Mexico ; museum databases
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In recent years, use of databases of the labels of specimens deposited in museums and herbaria is becoming increasingly common as a tool for addressing biodiversity conservation and management problems. These databases are often large in size and complex in structure, and their application to conservation deserves a wider appreciation of some of the biases, gaps and potential pitfalls common to them. In this paper, we discuss some of the problems associated with using such databases for obtaining lists of species for arbitrary sites, as well as for the estimation of the distribution area of single species. The possibility of obtaining these closely related variables using specimen databases is shown to be scale-dependent. A tool based on mark-recapture techniques is applied to the problem of: (i) detecting sites with low number of species due to lack of adequate in-site sampling and, (ii) species with small estimated areas due to poor spatial coverage of samples.
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    URL: http://dx.doi.org/10.1023/A:1008987010383
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    Attenuated tree species diversity in human-impacted tropical evergreen forest sites at Kolli hills, Eastern Ghats, India (2000)
    Springer
    Biodiversity and conservation 9 (2000), S. 1493-1519 
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    ISSN: 1572-9710
    Keywords: anthropogenic activities ; conservation ; Eastern Ghats ; permanent plot ; stand structure ; tree diversity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Tree diversity (≥30 cm gbh) in undisturbed and human-impacted tropical evergreen forest sites was investigated in the Kolli hills, Eastern Ghats, India. Four 2-ha contiguous permanent plots were erected, one each in Perumakkai shola (site PS), Vengodai shola (VS), Kuzhivalavu shola (KS) and Mottukkadu shola (MS) at 1000, 1050, 1200 and 1250 m elevation, with increasing human disturbance, to evaluate the difference in tree species composition, stand structure and dynamics. This paper discusses the results of the first survey. A total of 3825 individuals and 78 species from 61 genera and 36 families were enumerated in the 8 ha area. Among the four 2-ha sites, species richness was greatest (58) in the undisturbed site PS and lowest (39) in the highly disturbed site MS. Shannon, Simpson, Hill diversity and evenness indexes revealed a progressive reduction in diversity with increasing disturbance. The asymptote species-area curves imply adequate site sampling. Tree density (1151 to 651 trees ha−2) and basal area (106 to 46.6 m2 ha−2) decreased from undisturbed to disturbed site, due to selective felling. Single species, Memecylon umbellatum dominated sites MS (39%) and VS (26%), while Nothopegia heyneana, Memecylon umbellatum and Diospyros ovalifolia were dominant in PS, and Meliosma simplicifolia, Myristica dactyloides and Phoebe wightii in KS. Based on species abundance, we classify the study area as Memecylon–Phoebe–Beilschmiedia association with Neolitsea and Myristica as codominants. Tree population structure revealed a step-wise decline in girth frequencies with increasing size class in undisturbed site PS, whereas tree density fell sharp (〉50%) in medium girth class in the disturbed site MS. Population of the dominant species varied widely. The diversity values of this inventory are compared with similar studies in India and other tropical forests. Evidently, the reduction in species richness (by 52%), basal area (56%) and tree density (58%) in disturbed sites, with 57.6% of species rarity of this tropical evergreen forest, in secluded patches (‘sholas’) of Kolli hills, underlines conservation need to prevent species loss.
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    URL: http://dx.doi.org/10.1023/A:1008971015545
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    Diversity of surface dwelling beetle assemblages in open-cast lignite mines in Central Germany (2000)
    Springer
    Biodiversity and conservation 9 (2000), S. 1297-1311 
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    ISSN: 1572-9710
    Keywords: beetles ; conservation ; diversity ; open-cast lignite mining ; restoration ; succession
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We investigated species richness of ground dwelling beetle assemblages in two non-reclaimed lignite mines and a dump in Central Germany by means of pitfall trapping. During a period of five months, a total of 203 beetle species within 27 families represented by 4099 individuals were trapped. This included 75 species of ground beetles represented in a sample of 957 individuals from which 10 species are regionally endangered. The number of individuals, species richness, as well as the proportions of endangered species did not differ between successional stages whereas species composition of sites could be related well to a set of environmental variables. High values of beta-diversity between sites indicated that the total number of species recorded is caused by habitat diversity. From the viewpoint of nature conservation, we conclude that postmining areas can play a key role in conservation of beetle diversity in agricultural areas since they harbour threatened species whose original habitats are now rare due to human impact. An important task for future management of postmining areas is to maintain successional processes and to prevent loss of habitat diversity through afforestation. Areas with extreme soil conditions should also be preserved for long-term availability of bare soil and pioneer vegetation and associated fauna.
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    URL: http://dx.doi.org/10.1023/A:1008904605567
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    Planning for biodiversity conservation based on the knowledge of biologists (2000)
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    Biodiversity and conservation 9 (2000), S. 1153-1169 
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    ISSN: 1572-9710
    Keywords: biodiversity ; concepts ; conservation ; nature ; planning ; questionnaire survey
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract To conserve biodiversity, complementary approaches are necessary. Besides using museum data from sightings and specimens, the knowledge of experts can also be employed. Often such valuable information is lost on retirement or death. To investigate the value of this knowledge for nature conservation planning, we sent questionnaires to 124 professional conservationists in the province of KwaZulu-Natal, South Africa. Fifty-two replies illustrated that the historical context biases our concepts of nature and the conservation of biodiversity. Despite an awareness of all the spatial scales, complexities and dynamics of nature, there is still a strong focus on large-sized animals and visibly discrete ecosystems, such as wetlands. Nevertheless, the respondents illustrated that an awareness of infrequently-seen and less well known organisms is increasing. Harnessing this expert knowledge was valuable for conservation planning, but had the weakness that many taxa and localities were neglected. Similar problems arose with data from museum specimens. However, both these approaches were synergistic and highlighted the geographical areas that need far more exploration of their biodiversity. Such information gathering is an important ethical and practical exercise for conserving biodiversity.
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    Forest conservation and environmental management in Nepal: a review (2000)
    Springer
    Biodiversity and conservation 9 (2000), S. 1235-1260 
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    ISSN: 1572-9710
    Keywords: conservation ; environment ; forest ; management ; Nepal
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This paper provides information on the status of forests and environmental problems related to forestry in Nepal. It aims to integrate the conservation and sustainable management of forests as a part of environmental planning. A critical review of the main environmental problems with prioritization within the forestry sector; thorough review of all forest related documents, strategies, development plans, programs, institutional arrangements, policies mentioned in the Master Plan for Forestry Sector Nepal of 1988, and other relevant documents; identification of major gaps and constraints of forestry policy and plans and current practices of forest conservation and management have been done. The decline in forestry resources in Nepal took place in the past due to lack of appropriate policy to guide the legal, institutional and operational development for the forestry sector. Forestry policy in Nepal, in the past, was shaped by political and economic motives rather than ecological considerations. Policy formulation mechanisms exist in Nepal; however, there is an excessive delay in translating policies into legislation and then into operational rules and administrative orders. There is a need to implement the international obligations by transforming them into regulations in order to make them legally binding. The data to be generated in the coming years in the areas of forestry at both national and international levels suggest that we should revise the research priorities and strategies. Research to add value to the forest resource products has yet been neglected. More attention is required on regular monitoring and evaluation of the projects. A thorough evaluation of the programs and planning for the forestry sector in Nepal is urgently required to update the progress and revise the programs.
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    URL: http://dx.doi.org/10.1023/A:1008900216876
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    Waterbird communities along the estuarine salinity gradient of the Schelde estuary, NW-Europe (2000)
    Springer
    Biodiversity and conservation 9 (2000), S. 1275-1296 
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    ISSN: 1572-9710
    Keywords: communities ; conservation ; estuarine zonation ; salinity gradient ; Schelde estuary ; waterbirds
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The zonation of non-breeding waterbirds along the Schelde estuary (The Netherlands–Belgium), one of the longest estuaries in NW-Europe with still a complete salinity gradient, including a large freshwater tidal area, was described. Numbers of birds were counted monthly over the period October 1991 to June 1997. Highest numbers of waterbirds were observed in late autumn and winter, with annual peak numbers ranging between 150,000 and 235,000 individuals for the whole estuary. Based on a multivariate analysis different waterbird communities were observed along the salinity gradient. The polyhaline areas of the estuary were numerically dominated by the waders Oystercatcher and Dunlin. Due to the presence of a large brackish marsh in the mesohaline zone, the waterbird community in this area was dominated by the herbivores Wigeon and Greylag Goose. In the oligohaline and freshwater tidal areas, the waterbird community was dominated by duck species, with Teal and Mallard being the most important. The international importance of the Schelde estuary for waterbirds was evidenced by the fact that for 21 waterbird species the 1% level criterion, according to the Ramsar convention, was exceeded. The relation of the observed diversity and community patterns with the functional and habitat diversity of the Schelde estuary as well as the effect of recent conservation measures to preserve this habitat were discussed.
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    The Muni-Pomadze Ramsar site (2000)
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    Biodiversity and conservation 9 (2000), S. 447-464 
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    ISSN: 1572-9710
    Keywords: biodiversity ; conservation ; Ghana ; RAMSAR ; wetland
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Coastal Wetlands Management Project (CWMP), funded by the Global Environment Facility and implemented by the Ghana Wildlife Department, seeks to preserve the ecological integrity of coastal lagoons that serve as important sites for migratory waterbirds. This report describes the geomorphology, hydrology, soils, water chemistry, and vegetation of the Muni-Pomadze Ramsar site. Muni lagoon is a saline, shallow water lagoon separated from the sea by a sand bar which may be breached occasionally. The bulk of the rainfall in the catchment evaporates or flows as surface runoff into three streams that empty into Muni lagoon. Open water in the lagoon varies seasonally from 100 ha in the dry season to over 1000 ha in the wet season. The natural flora of the site can be divided into four main types; flood plain (including mangrove and wetland vegetation), dune vegetation, riverine vegetation, and terrestrial vegetation on elevated ground. The latter consists of a combination of grasslands, thickets, and Eucalyptus plantations. Fifty-three percent of the site is classified as natural vegetation. An additional 32.5% is agricultural land and 12.6% is residential area for the 11 communities within the site. The main source of employment are farming or fishing. The area surrounding Muni lagoon is used extensively for bushmeat hunting and as the tribal hunting grounds of the Efutu people. Apart from the global importance of the Muni-Pomadze site for biodiversity, management of the site is further justified by its considerable potential for development as an income-generating and educational nature reserve with an eco-cultural theme, managed by the local communities in partnership with the governmental and non-governmental conservation agencies.
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    Plant species diversity and tree population structure of a humid tropical forest in Tamil Nadu, India (2000)
    Springer
    Biodiversity and conservation 9 (2000), S. 1643-1669 
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    ISSN: 1572-9710
    Keywords: conservation ; plant diversity ; population structure ; regeneration ; tropical forest ; Western Ghats
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Vegetation structure and species composition of tropical ecosystems were studied through nine transects at Veerapuli and Kalamalai reserve forests in the Western Ghats of Tamil Nadu, India. Species diversity, dominance, species richness and evenness indices of plant communities and also population structure of woody plants were enumerated. A total of 244 species (183 genera and 76 families) were recorded. Species richness (number of species) were 82,142 and 96 species per 0.3 ha respectively for the study areas of low-elevation forest (LEF), mid-elevation forest (MEF) and high elevation forest (HEF). Species diversity indices were greater in MEF compared to the other two forests except juveniles. In contrast, greater dominance value indices were recorded in LEF than other forests. Density and basal area of the MEF were twice greater than the LEF, while HEF showed greater tree density and low basal area when compared to LEF. The stem density and species richness (number of species) decreased with increased size classes of trees observed in the present study indicated good regeneration status. Population structure of juveniles and seedlings also reflects good regeneration status. Terminalia paniculata (IVI of 99.9) and Hopea parviflora (IVI of 103.8) were dominant tree species respectively in LEF and MEF whereas in HEF Agrostistachys meeboldii (63.65), Cullenia excelsa (63.67) and Drypetes oblongifolia (39.67) share the dominance. Past damage (anthropogenic perturbation) may be one of the reasons for single species dominance in LEF and MEF. Occurrence of alien species such as Eupatorium odoratum and Ageratum conyzoides also indicated the past disturbance in LEF. The variations in plant diversity and population structure are largely due to anthropogenic perturbation and other abiotic factors.
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    Conservation of biodiversity in Scandinavian boreal forests: large carnivores as flagships, umbrellas, indicators, or keystones? (2000)
    Springer
    Biodiversity and conservation 9 (2000), S. 857-868 
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    ISSN: 1572-9710
    Keywords: biodiversity ; boreal forest ; carnivore ; conservation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Large carnivores are often used as focal species (indicators, umbrellas, flagships or keystones) in conservation strategies either aimed at conserving carnivores, the rest of the biodiversity that occupies their habitats, or both. We evaluate their suitability for these roles in the context of boreal forest biodiversity conservation in the muti-use landscapes of Scandinavia. The enormous conflicts, especially with livestock, that carnivores cause in these areas makes them very controversial flagships to the extent that it may affect rural people's attitudes to conservation in general. Because of the broad habitat tolerance of large carnivores and their prey, and the difficulties in surveying carnivore numbers, they are very insensitive and impractical indicators of forest biodiversity. This ability of large carnivores to thrive in industrial forests means that the many species that are sensitive to modern forestry will not fall under the umbrella of areas managed for large carnivores. If large carnivores have a keystone function with respect to affecting the density of their ungulate prey it is likely to lead to even further conflicts with hunters who gain economic benefit from harvesting wild ungulates. In other words, none of the classic `ecological' arguments are likely to help justify large carnivore conservation, and large carnivore conservation is unlikely to help conserve the rest of the boreal forest's biodiversity. Based on these arguments we recommend that (1) justification for large carnivore conservation focus on the real philosophical and value orientated reasons rather than ecological justifications, (2) that this conservation should be brought about in practice by dedicated management programs that specifically address the conflicts caused by large carnivores, and (3) that boreal forest biodiversity is best conserved by specific actions designed to establish reserves or change forestry practices.
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    Leaf litter inhabiting beetles as surrogates for establishing priorities for conservation of selected tropical montane cloud forests in Honduras, Central America (Coleoptera; Staphylinidae, Curculionidae) (2000)
    Springer
    Biodiversity and conservation 9 (2000), S. 617-653 
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    ISSN: 1572-9710
    Keywords: beetles ; biodiversity ; cloud forest ; conservation ; Curculionidae ; Honduras ; Staphylinidae ; tropical forest
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Thirteen tropical montane cloud forests (TMCFs) in Honduras and adjacent El Salvador were evaluated for species diversity and endemism in leaf litter inhabiting Curculionidae (weevils) and Staphylinidae in June and August of 1994. Totals of 26,891 specimens representing 293 species of Curculionidae, and 7349 specimens representing 224 species of Staphylinidae, were collected. Regional endemism was high with 173 species (58.7% of total) of Curculionidae and 126 species (56.3% of total) of Staphylinidae, restricted to single sites. Measures of diversity (number of observed species [Sobs]; number of endemic species) and estimates of biodiversity (Chao 2, first and second order Jackknife and Bootstrap richness estimators) are given for each site for Curculionidae and Staphylinidae. Priority-areas analyses based on Sobs (‘Greedy’), Sobs, Chao 2, number of endemic species, site complementarity and higher taxonomic diversity (Curculionidae only) are presented. Weak or lack of correlation was noted between site area and site diversity for either Curculionidae or Staphylinidae. The optimum sequence for site conservation was determined based on Sobs (‘Greedy’). Four sites are identified as the highest conservation priorities; Parque Nacional Montecristo, P.N. La Muralla, P.N. Santa Barbara and P.N. Comayagua. Identification as high priority sites supports their designation as Parques Nacionales rather than the less diverse Reservas Biologias or Reservas Vidas Silvestres. While closely approximating performance of Sobs (‘Greedy’), no one of Sobs, number of endemics, Chao 2 or site complementarity give results equivalent to the optimum sequence based on Sobs (‘Greedy’) and the latter is concluded to be the best method for establishing conservation priorities in TMCF. Results of the analyses based on one of Curculionidae or Staphylinidae differ in the ordering of site priorities based on each of Sobs, Chao 2 or number of endemics at each site, with at best, weak positive correlations between results based on each taxon. Data from other taxa are necessary to determine if one of Curculionidae or Staphylinidae emerges as the better surrogate for general patterns of biodiversity in TMCF. Conservation strategies in Central America should emphasize the importance of TMCF particularly in view of high regional endemism. In formulating these strategies, consideration needs to be given to the preservation of many small regional preserves rather than fewer larger preserves. Data from inventories of other taxa should be included where available and all biological data should be integrated with social and cultural issues of regional importance.
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    Species diversity of Amphibia, Reptilia and Lipotyphla (Mammalia) at Ambolokopatrika, a rainforest between the Anjanaharibe-Sud and Marojejy massifs, NE Madagascar (2000)
    Springer
    Biodiversity and conservation 9 (2000), S. 1587-1622 
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    ISSN: 1572-9710
    Keywords: Amphibia ; biodiversity ; conservation ; Lipotyphla ; Madagascar ; rainforest corridor ; Reptilia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Réserve Spéciale d' Anjanaharibe-Sud and the Parc National de Marojejy are two important areas of biodiversity and endemicity in northeastern Madagascar. These reserves are separated by about 40 km, including the extensive Andapa Basin, and connected by a rather narrow mid-altitude montane ridge. Nothing was previously known about the biota of this corridor and its faunal relations with the two reserves. At this purpose, during 1997 the Ambolokopatrika rainforest (lying about midway between Anjanaharibe-Sud and Marojejy massifs) was surveyed for amphibians, reptiles, and for small mammals belonging to the order Lipotyphla. As a general rule these vertebrates may be important ecological indicators, while the herpetofauna (Amphibia, Reptilia) exhibits a high degree of habitat specialisation and endemicity. Furthermore, the herpetofauna and lipotyphlans of Ambolokopatrika Forest were compared to those known from the forests of Anjanaharibe-Sud, Marojejy, and Tsararano massifs, the latter site being a southern extension of the Anjanaharibe-Sud chain. These animals were surveyed by use of opportunistic searching and pitfall trapping during two seasonal periods, May–June (winter), and November–December (summer). Forty-two species of amphibians, 23 of reptiles, and nine of lipotyphlans were recorded at Ambolokopatrika Forest. The biodiversity of Ambolokopatrika is comparable to those of other analysed sites, and this stresses the value of this forest in assuring biotic exchange between Anjanaharibe-Sud and Marojejy reserves. Considerations are also provided on the faunal similarities and differences in terms of exclusivity and endemisms. It is therefore suggested that a certain degree of protection should be given to Ambolokopatrika rainforest, to assure a biological connection and exchange between the protected areas of Anjanaharibe-Sud and Marojejy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1026559728808
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    Biological Diversity - an Overview (2000)
    Springer
    Water, air & soil pollution 123 (2000), S. 299-310 
    Details
    ISSN: 1573-2932
    Keywords: biodiversity ; conservation ; ecosystems ; gene banks ; Israel
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Despite the overwhelming importance of biodiversity and the growing demand for the genes and chemicals, biodiversity is being lost at alarming rates, largely as a result of human action enhancing degradation of biologically rich ecosystems like tropical rain forests, grassland and coral reefs. A positive global change was achieved at the Earth Summit in Rio, 1992, requiring all nations to take four basic steps: develop national strategies for conservation, establish a system of protected areas, begin to rehabilitate damaged ecosystems and integrate conservation policy into national decision making. The conservation of biological diversity, sustainable use, technology transfer, intellectual property rights, provision of financing and the principle of equitable sharing of benefits were also promoted. On the local scene, Israel is known for its rich natural vegetation and diversified species which contribute to the biological diversity and plant species population. Beyond the intrinsic value, the diversified plant population is an extremely valuable genetic source for improvement of agricultural crops and extraction of new drugs. The Israeli Gene Bank (IGB) was established to preserve the natural endowment, and is responsible for in-situ conservation, formation of gene banks and other related activities with emphasis on regional and international cooperation.
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    URL: http://dx.doi.org/10.1023/A:1005268804246
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    Determination of Pollutant Phenols by Capillary High-Performance Liquid Chromatography with UV Detection (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 367-372 
    Details
    ISSN: 0935-6304
    Keywords: Capillary HPLC ; gradient elution ; temperature programming ; phenolic compounds ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---This paper describes a liquid chromatographic method using a reversed phase capillary column coupled to an UV detector for the quantitation of thirteen pollutant phenols. Chromatographic separation was carried out with gradient elution at 25.0 ± 0.1°C. The two major anisocratic elution modes (gradient elution and temperature programming) were evaluated. The detection limit range was 10-81 pg (100 nL injected). The chromatographic method combined with liquid-liquid extraction was applied to analysis of these compounds in river water. Recoveries of 75-103% were achieved for most of them.
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    Use of Capillary Supercritical Fluid Chromatography in the Analysis of Zinc Dialkyldithiophosphates (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 389-392 
    Details
    ISSN: 0935-6304
    Keywords: Capillary SFC ; zinc dialkyldithiophosphates ; lubricating oils ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: No abstract
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    Application of Supercritical Fluid Chromatography to the Analysis of Waxes in Objects of Art (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 393-396 
    Details
    ISSN: 0935-6304
    Keywords: Supercritical fluid chromatography (SFC) ; micropacked capillary columns ; waxes ; restoring ; objects of art ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: No abstract
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    Identification of Free and Glycosidically Bound Volatiles and Glycosides by Capillary GC and Capillary GC-MS in “Lulo del Chocó” (Solanum topiro) (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 379-385 
    Details
    ISSN: 0935-6304
    Keywords: Aroma ; “lulo del Chocó” ; Solanum topiro ; volatiles ; glycosidically bound volatiles ; glycoside TFA derivatives ; enantioselective gas chromatography ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---The volatile constituents of lulo del Chocó (Solanum topiro) fruit pulp obtained by liquid-liquid extraction were analyzed by capillary GC and capillary GC-MS. In total, 30 components were identified with methyl salicylate, hexadecanoic acid, hexanal, guaiacol, ethyl butanoate, and ethyl acetate being the major components. Chirospecific MDGC analysis revealed the predominance of (R)-ethyl-3-hydroxybutanoate (ee 40%) and the presence of racemic mixtures both of δ-octalactone and of δ-decalactone. For γ-hexalactone, γ-octalactone, and γ-decalactone enantiomeric distributions of 22.4 : 77.6, 22.9 : 77.1, and 20.0 : 80.0, (R) : (S), respectively, were determined. Glycosidically bound aroma compounds were identified by capillary GC and capillary GC-MS after isolation of the glycosidic fraction obtained by Amberlite XAD-2 adsorption and methanol elution followed by hydrolysis with a commercial pectinase enzyme. In total 13 bound aroma compounds (aglycones) were identified. These aglycones mainly consisted of compounds exhibiting aromatic structures. Additionally, with the aid of capillary GC and capillary GC-MS (EI and NCI) of trifluoroacetylated derivatives we identified eight glucosides: the novel 3,6-epoxy-7-megastigmen-5,9-diol β-D-glucopyranoside and the hexyl, benzyl, linalyl oxide (furanic), 2-phenylethyl, vomifolyl (isomer 1), (6S,9R)-vomifolyl, and scopoletin β-D-glucopyranosides.
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    System Peaks of Cyclodextrins in Capillary Electrophoresis (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 409-412 
    Details
    ISSN: 0935-6304
    Keywords: Cyclodextrin ; capillary electrophoresis ; system peak ; CHES 1 ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Using a running buffer containing cyclodextrins (CDs) and 2-[N-cyclohexylamino]-ethanesulfonic acid (CHES), positive system peaks were observed in the analysis of a ganglioside mixture by CE-UV. These system peaks were related to CDs in the running buffer because these peaks were also detected when a plug of solution devoid of CDs but having the same CHES concentration and pH as the running buffer was injected. Neutral CDs were separated owing to the formation of inclusion complexes with the anionic CHES ion. One possible explanation for the positive system peaks is that the anionic CD-CHES inclusion complex is displaced by co-ions with higher UV absorptivity.
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    Liquid Chromatography of Unstable Zinc Dithiocarbamates. Application to Rubber Gloves Analysis (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 565-566 
    Details
    ISSN: 0935-6304
    Keywords: Liquid chromatography ; zinc dithiocarbamate ; vulcanization accelerator ; rubber gloves ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: No abstract
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    Characteristic Stationary Phase Constants for Two Popular Open-Tubular Column Stationary Phases for Gas Chromatography (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 603-608 
    Details
    ISSN: 0935-6304
    Keywords: Solvation parameter model ; ystem constants for poly(dimethyldiphenylsiloxane) HP-5 and poly(ethylene glycol) HP-INNOWax stationary phases ; temperature ; molecular interaction ; retention models ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---No abstract
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    Determination of Cinnamomi Constituents by High-performance Liquid Chromatography and Capillary Electrophoresis (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 561-564 
    Details
    ISSN: 0935-6304
    Keywords: Retention factors ; Cinnamomi ramulus ; high-performance liquid chromatography ; capillary electrophoresis ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---No abstract
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    Ion Chromatography of Inorganic Anions on Graphitic Carbon as the Stationary Phase (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 590-594 
    Details
    ISSN: 0935-6304
    Keywords: Ion chromatography ; graphitic carbon stationary phases ; anions ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---Inorganic anions could be separated on porous graphitic carbon stationary phases in ion chromatography. Ion exchange between eluent anions and sample anions on the stationary phase was confirmed by the retention behavior and the possibility of indirect photometric detection. The elution order of anions was different from that observed for commercially available anion exchangers. Chloride, nitrate, and sulfate contained in tap water could be determined in 7 min.
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    Solid-Phase Microextraction of Pyrethroid Pesticides from Water at Low and Sub-ppt Levels at Different Temperatures (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 485-488 
    Details
    ISSN: 0935-6304
    Keywords: Pyrethroids ; solid-phase microextraction ; water analysis ; temperature ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---Synthetic pyrethroids are increasingly being used for insect control on field crops because of their advantageous environmental properties such as short field life, broad spectrum of insecticide activity, and relatively low mammalian toxicity. In the present work, experimental conditions were optimized for trace analysis of pyrethroids in water by using solid-phase microextraction followed by capillary gas chromatography. Effects of temperature, SPME-fiber diameter, and matrix were studied. SPME used off-line with a gas chromatograph electron-capture detection (GC-ECD) showed a linear response over a wide concentration range. Our results showed a strong temperature effect on pyrethroid extraction yield. The detection limit after 5 min of SPME-fiber exposure to the samples changed from 1 ppb (at 30°C) to 0.1 ppt (at 90°C), which is a 10000 increase in pyrethroid detection. The standard deviations of the analyte peak area ratios were typically in the range of 1-7% at 30°C and of 3 to 15% at 90°C. Best conditions for pyrethroids analysis were achieved using high temperature solution and a 100 μm polydimethylsiloxane fiber. No matrix (river water) influence was observed on detection of the investigated pyrethroids.
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    Microfabricated Monolith Columns for Liquid Chromatography. Sculpting Supports for Liquid Chromatography (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 19-26 
    Details
    ISSN: 0935-6304
    Keywords: Microfabrication ; monolith ; electrochromatography ; peptides ; reactive ion etching ; reversed phase ; nanocolumns ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Monolith columns are generally fabricated by polymerization of monomers within a column. This paper reviews an alternative strategy in which the bed is microfabricated in an inorganic material by ablation. Channels of 1.5 μm width and 10 μm depth were sculpted in quartz by deep reaction ion etching. Using this approach chromatographic beds were constructed in which cubic support structures were created and arranged in rows to mimic particles in a conventional column. Beds ranging from hundreds of thousands to millions of “particles” with volumes of 15 nL to 15 μL were produced. Columns that had been derivatized with an octadecyl silane stationary phase were used to separate both low molecular weight analytes and peptides in the CEC mode. Plate height in the CEC mode was 1.2 μm at maximum efficiency.
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    Dynamic Capacity Studies of CIM (Convective Interaction Media)® Monolithic Columns (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 39-43 
    Details
    ISSN: 0935-6304
    Keywords: Monoliths ; CIM® monolithic column ; dynamic binding capacity ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The characterization of CIM® DEAE monolithic columns in terms of dynamic binding capacity is presented in this paper. Breakthrough experiments were performed for capacity determination. Bovine serum albumin (BSA) was used as a model protein. It is shown that CIM® monolithic columns have good batch-to-batch reproducibility as well as long-term stability. The experiments performed under different linear velocities demonstrated that the dynamic capacity is unaffected at least up to a linear velocity of 2450 cm/h. Furthermore, the breakthrough curve slope is constant, indicating that the capacity would remain constant at even higher linear velocities. The adsorption isotherm of BSA dissolved in 20 mM Tris-HCl buffer shows a constant capacity of around 30 mg/mL of support down to a concentration of 20 μg/mL. The capacity is substantially influenced by the ionic strength; however, 20% of the maximal capacity is still preserved at 0.3 M NaCl.
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    Affinity Chromatography of Human Blood Coagulation Factor VIII on Monoliths with Peptides from a Combinatorial Library (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 47-58 
    Details
    ISSN: 0935-6304
    Keywords: Monolith ; factor VIII ; affinity chromatography ; combinatorial peptide library ; peptide synthesis ; recombinant proteins ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: FVIII is a very complex molecule of great therapeutic significance. It is purified by a sequence of chromatographic steps including immunoaffinity chromatography. A peptide affinity chromatography method has been developed using peptides derived from a combinatorial library. Spot technology using cellulose sheets has been applied for this purpose. The dual positional scanning strategy was used for identification of the amino acids in random positions. Approximately 5000 possible candidates found in the first screening round were reduced to a panel of 36. Six candidates have been selected empirically. Five peptides seem to be directed against the light chain of FVIII, one peptide seems to be directed against the heavy chain. The peptides have been immobilized on conventional beaded material and CIM polymethacrylate monoliths. Much better performance with respect to capacity and selectivity has been observed with the monolithic material. Exposure of the ligand and its ensuing accessibility are responsible for these properties.
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    Capillary Electrochromatography with Monolithic Poly(styrene-co-divinylbenzene-co-methacrylic acid) as the Stationary Phase (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 67-72 
    Details
    ISSN: 0935-6304
    Keywords: Capillary electrochromatography ; monolithic column ; electroosmotic flow ; retention mechanism ; aromatic compound ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A new kind of monolithic capillary electrochromatography column with poly(styrene-co-divinylbenzene-co-methacrylic acid) as the stationary phase has been developed. The stationary phase was found to be porous by scanning electron microscopy and the composition of the continuous bed was proved by IR spectroscopy to be the ternary polymer of styrene, divinylbenzene, and methacrylic acid. The effects of operating parameters, such as voltage, electrolyte, and organic modifier concentration in the mobile phase on electroosmotic flow were studied systematically. The retention mechanism of neutral solutes on such a column proved to be similar to that of reversed-phase high performance liquid chromatography. In addition, fast analyses of phenols, chlorobenzenes, anilines, isomeric compounds of phenylenediamine and alkylbenzenes within 4.5 min were achieved.
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    Continuous-Bed Columns Containing Sol-Gel Bonded Packing Materials for Capillary Electrochromatography (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 73-80 
    Details
    ISSN: 0935-6304
    Keywords: Continuous-bed ; sol-gel ; octadecylsilica ; mixed-mode stationary phase ; large-pore ; column technology ; capillary electrochromatography ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---Sol-gel bonded packing materials in continuous-bed columns have been prepared for capillary electrochromatography (CEC). Three packing materials were investigated: small-pore Spherisorb ODS1 (3 μm, 80 Å) with octadecyl as stationary phase, small-pore mixed-mode Spherisorb ODS/SCX (3 μm, 80 Å) with octadecyl and propyl sulfonic acid as stationary phases, and large-pore Nucleosil ODS (7 μm, 1 400 Å) with octadecyl as stationary phase. The characteristics of these columns were compared in terms of electroosmotic flow, efficiency, inertness, and retention factors. In contrast to columns containing sol-gel bonded ODS, columns containing sol-gel bonded mixed-mode ODS/SCX generated nearly pH independent electroosmotic flow (EOF) over pH 2-9. Columns containing sol-gel bonded large-pore ODS produced nearly three times lower reduced plate height than those containing small-pore ODS. Efficiencies of 220,000 plates per meter and 175,000 plates per meter were obtained from columns containing sol-gel bonded 7 μm, 1 400 Å ODS and columns containing sol-gel bonded 3 μm, 80 Å ODS, respectively, which are among the highest reported efficiencies for continuous-bed columns. In CEC, over one million plates per meter and pH independent EOF are expected from continuous-bed columns containing sol-gel bonded 1.5 μm particles with large pores and mixed-mode stationary phases.
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    Particle Loaded Monolithic Sol-Gel Columns for Capillary Electrochromatography: A New Dimension for High Performance Liquid Chromatography (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 81-88 
    Details
    ISSN: 0935-6304
    Keywords: Monolithic chromatography columns ; capillary electrochromatography (CEC) ; micro high performance liquid chromatography ; sol-gel based columns for chromatography ; fritless capillary chromatography columns ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---Particle-loaded (3 μm, octadecylsilica) monolithic sol-gel columns have been prepared and selected characteristics measured. Several electrical properties may be calculated from simple current measurements in the column as a whole. Resistivity in the packed segment is approximately three times that in open segments, resulting in a 60% increase in field strength in the packed regions compared to the capillary with no packing. The surprisingly high specific permeability of these sol-gel columns is characteristic of 8-μm particles, which allows their operation in the microLC mode at pressures as low as 69 kPa where their efficiency is about 50,000 plates per meter and in the CEC mode where efficiency is about 106,000 plates per meter at 5 kV. There is a relatively rapid loss of efficiency with increasing linear velocity beyond 0.2 mm/s in microLC mode, which may be due to additional diffusion processes in the inter-particulate voids. A rapid loss of efficiency above 0.5 mm/s is also observed in the CEC mode, for the same reasons. Chromatographic retention behavior in either separation mode is characteristic of conventional octadecylsilica particles, indicating that analytes have significant access to the surface within the pores of the immobilized bonded phase.
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    Preparation of Fritless Packed Silica Columns for Capillary Electrochromatography (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 89-92 
    Details
    ISSN: 0935-6304
    Keywords: Capillary electrochromatography ; packed columns ; fritless columns ; stationary phases ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---Fritless packed silica gel columns were prepared using sol-gel technology. A part of a 75 μm i.d. fused silica capillary was filled with a mixture of tetramethoxysilane and poly(ethylene glycol). After gelling at 40°C and heating at 300°C, the resultant silica gel was derivatized with dimethyloctadecylchlorosilane. A scanning electron micrograph of a cross-section of the capillary column showed that the gel took the form of a spherical particle aggregate and adhered to the column inner wall. The column performance was evaluated for electrochromatography using acetonitrile-50 mM HEPES buffer (pH 6.6) (60/40 or 40/60, v/v) as the mobile phase. An electroosmotic flow of 1.0 mm/s was generated with (60/40, v/v) acetonitrile/HEPES buffer at a field strength of 546 V/cm. Using a sol-gel-derived packed column at an electroosmotic flow of 0.5 mm/s, efficiencies of up to 1.1×105 plates/m were obtained for retained solutes.
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    A New Monolithic-Type HPLC Column For Fast Separations (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 93-99 
    Details
    ISSN: 0935-6304
    Keywords: HPLC ; monolithic column ; silica rod column ; fast separations ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---The application of a new silica-based, monolithic-type HPLC-column for fast separations is presented. The column is prepared according to a new sol-gel process, which is based on the hydrolysis and polycondensation of alkoxysilanes in the presence of water soluble polymers. The method leads to “rods” made of a single piece of porous silica with a defined pore structure, i. e. macro- and mesopores. The main feature of silica rod columns is a higher total porosity, about 15% higher than of conventional particulate HPLC columns. The resulting column pressure drop is therefore much lower, allowing operation at higher flow rates including flow gradients. Consequently, HPLC analysis can be performed much faster, as it is demonstrated by various applications.
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    Monolithic Stationary Phases for Capillary Electrochromatography Based on Synthetic Polymers: Designs and Applications (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 3-18 
    Details
    ISSN: 0935-6304
    Keywords: Capillary electrochromatography ; monolithic columns ; synthetic polymers ; stationary phase ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Monolithic materials have quickly become a well-established stationary phase format in the field of capillary electrochromatography (CEC). Both the simplicity of their in situ preparation method and the large variety of readily available chemistries make the monolithic separation media an attractive alternative to capillary columns packed with particulate materials. This review summarizes the contributions of numerous groups working in this rapidly growing area, with a focus on monolithic capillary columns prepared from synthetic polymers. Various approaches employed for the preparation of the monoliths are detailed, and where available, the material properties of the resulting monolithic capillary columns are shown. Their chromatographic performance is demonstrated by numerous separations of different analyte mixtures in variety of modes. Although detailed studies of the effect of polymer properties on the analytical performance of monolithic capillaries remain scarce at this early stage of their development, this review also discusses some important relationships such as the effect of pore size on the separation performance in more detail.
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    Preparative Monolithic Silica Sorbents (PrepRODs™) and Their Use in Preparative Liquid Chromatography (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 100-105 
    Details
    ISSN: 0935-6304
    Keywords: Preparative chromatography ; silica monoliths ; PrepROD™ ; productivity ; SMB-chromatography ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---The use of monolithic silica sorbents for the isolation of substances by preparative liquid chromatography is demonstrated. Preparative liquid chromatography is recognized as a valuable technique for the isolation and purification of substances in the pharmaceutical and fine chemicals industry. The system technology has meanwhile reached a high standard, and the greatest future improvements are expected to arise from new and improved adsorbents. Monolithic silica sorbents offer some unique features for preparative liquid chromatography. They exhibit high efficiencies even at high flow rates due to their fast convective mass transfer and can therefore be used at very high mobile phase velocities, leading to high productivity and hence to maximum process economy. The benefits of this new type of adsorbent are illustrated for an example in batch-chromatographic mode and an example using the continuous simulated moving bed (SMB) technology.
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    Tailoring Mesopores in Monolithic Macroporous Silica for HPLC (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 106-110 
    Details
    ISSN: 0935-6304
    Keywords: Silica ; monolithic column ; aging ; mesopores ; pore size distribution ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---Mesopore formation in silica gels having continuous macropores has been investigated. The macroporous wet silica gel prepared by the sol-gel process including phase separation was aged in a basic solvent making use of hydrolysis of urea in a closed condition. The mesopore structure was finally obtained by subsequent evaporation drying of solvent and heat-treatment at 600°C for 2 h. The dissolution-reprecipitation kinetics at the interfaces between wet gel skeletons and an external solvent affected the size and volume of pores formed within the skeletons. Below 120°C, mesopores suitable for various chromatographic applications have been formed typically within 24 h. On the other hand, at 200°C, the pore size attained the macropore dimensions (〉50 nm), and the whole macroporous morphology was significantly modified.
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    Monolithic Silica Columns for HPLC, Micro-HPLC, and CEC (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 111-116 
    Details
    ISSN: 0935-6304
    Keywords: Monolithic column ; silica monolith ; HPLC ; capillary liquid chromatography ; capillary electrochromatography ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---Two types of monolithic silica columns derivatized to form an ODS phase, one prepared in a fused silica capillary (SR-FS) and the other prepared in a mold and clad with an engineering plastic (poly-ether-ether-ketone) (SR-PEEK), were evaluated. The column efficiency and pressure drop were compared with those of a column packed with 5-μm ODS-silica particles and of an ODS-silica monolith prepared in a mold and wrapped with PTFE tubing (SR-PTFE). SR-FS gave a lower pressure drop than a column packed with 5-μm particles by a factor of 20, and a plate height of 20 μm at a linear velocity below 1 mm/s. SR-PEEK showed higher flow-resistance than the other monolithic silica columns, but they still showed a minimum plate height of 8-10 μm and a lower pressure drop than popular commercial columns packed with 5-μm particles. The evaluation of SR-FS columns in a CEC mode showed much higher efficiency than in a pressure-driven mode.
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    Note on the Net Retention Volume in Chromatography with a Real Gas as a Carrier (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 119-126 
    Details
    ISSN: 0935-6304
    Keywords: Gas chromatography ; retention volume ; real gas carrier ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The problem of the calculation and interpretation of the net retention volume with a real gas as carrier is revisited. The properties of the carrier are described by a first order virial equation of state. The net retention volume of a solute is related to the mean flow rate of the carrier, therefore determination of the mean flow rate of a real gas carrier is reviewed. It is shown that the mean flow rate cannot be calculated from the mean column pressure. With a real gas carrier the local capacity factor depends on the local pressure of the real gas. The basic relationship between the net retention volume and the function describing this pressure dependence is also reviewed. Precise formulae as well as practical approximations are presented for the calculation of the mean flow rate, of the mean column pressure, and of a representative pressure related to the mean capacity factor.
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    Detection of Polyols by HPLC Coupled with an On-Line Photochemical Oxidation Reactor and Chemiluminescence Detection (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 127-130 
    Details
    ISSN: 0935-6304
    Keywords: Chemiluminescence ; diol compound ; oxalate ; post-column reaction ; ruthenium complex ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---In this paper we propose a new post-column detection method for polyols containing 1,2-diol, 1,3-diol, and saccharides. The polyols are oxidized in a photochemical reactor to yield oxalate with subsequent chemiluminescence detection using [Ru(III)(bpy)3]3+. A mixing solution of eluate and oxidizing reagent is delivered to a reaction coil, which is then irradiated with ultraviolet light to promote the oxidation reaction. The detection limits for 1,2-ethanediol (ethylene glycol) and 1,3-propanediol were 38 pmol and 23 pmol, respectively.
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    Coupled LC-GC-NPD for Determination of Carbazole-Type PANH and Its Application to Personal Exposure Measurement (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 131-137 
    Details
    ISSN: 0935-6304
    Keywords: On-line coupled LC-GC ; nitrogen selective detection ; NPD ; carbazoles ; PANH ; personal exposure measurement ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A coupled LC-GC method for the analysis of carbazole-type PANH has been developed and evaluated. Group separation and isolation of carbazoles from interfering acridines in a complex sample matrix was accomplished by using a back-flush technique and an in situ end-capped dimethylaminopropyl silica column in the HPLC part of the system. On-line injection of the carbazole fraction into the GC column was performed with a loop-type interface utilizing concurrent solvent evaporation technique. An LOD of 1-3 pg of individual carbazole compounds was achieved by nitrogen selective detection using an NPD. The method is shown to be robust and is demonstrated by application to personal exposure measurement in an aluminum reduction plant.
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    Fast Separation of Nucleosides by Capillary Electrochromatography on Non-Endcapped Phenyl-Bonded Silica Phase Using Short-End Injection Method (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 138-142 
    Details
    ISSN: 0935-6304
    Keywords: Capillary electrochromatography ; nucleosides ; short-end injection ; phenyl-bonded silica ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---The influence of several experimental parameters (pH, ionic strength, organic modifier content of hydro-organic buffer) upon EOF, migration time, and retention factor has been studied in CEC with a phenyl-bonded silica column on a model mixture of five nucleosides. This paper illustrates the current interest in CEC as a method of resolving complex mixtures of neutral and ionic solutes and demonstrates the potential of the short-end injection method as a means of reducing analysis time.
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    Separation of Anthraquinones by Capillary Electrophoresis and High-Performance Liquid Chromatography (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 143-148 
    Details
    ISSN: 0935-6304
    Keywords: Analysis of anthraquinones ; capillary electrophoresis ; high-performance liquid chromatography ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---The separation and determination of twelve anthraquinones, viz. anthraquinone 1, chrysphanol 2, aloe-emodin 3, alizarin 4, anthraquinone-2-carboxylic acid 5, purpurin 6, sennoside B 7, sennoside A 8, emodin 9, quinalizarin 10, rhein 11, and anthraflavic acid 12, were achieved by capillary electrophoresis (CE) and high-performance liquid chromatography (HPLC). Detection at 260 nm with a buffer solution containing 30 mM sodium borate (adjusted to pH = 10.56 with 0.05N NaOH) and acetonitrile (9 : 1) in CE or with a linear gradient elution containing 20 mM KH2PO4 with 0.05% phosphoric acid (pH = 2.91) and methanol in HPLC was found to be the most suitable approach for this separation. Contents of six components (2, 3, 7, 8, 9, 11) in crude Rhei Rhizoma extract could easily be determined within 39 min by CE or 63 min by HPLC. The effects of buffers on this separation and the validation of the two methods were studied.
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    Capillary Gas Chromatography of Trichlorophenols Using Ammonia as Carrier Gas (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 156-157 
    Details
    ISSN: 0935-6304
    Keywords: Capillary gas chromatography ; trichlorophenols ; ammonia ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---No abstract
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    Large Volume Injection in Fast Gas Chromatography with On-Column Injector (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 149-155 
    Details
    ISSN: 0935-6304
    Keywords: Capillary GC ; fast GC ; on-column injection ; narrow-bore column ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---In this work a fast gas chromatography set-up with on-column injection was optimized and evaluated with a model mixture of C8-C28 n-alkanes. Usual injection volumes when using narrow-bore (e. g., 0.1 mm i.d.) analytical columns are ca. 0.1 μL. The presented configuration allows introduction of 10-30-fold larger sample volumes without any distortion of peak shapes. In the set-up a normal-bore retention gap (1 m×0.32 mm i. d.) was coupled to a narrow-bore (4.8 m×0.1 mm i. d.×0.4 μm film thickness) analytical column using a low dead volume column connector. The effects of the experimental conditions such as inlet pressure, sample volume, initial injection temperature, and oven temperature on a peak focusing are discussed. H-u curves for helium and hydrogen are used to compare their suitability for high speed gas chromatography and to show the dependence of separation efficiency on the carrier gas velocity at high inlet pressures. In the fast gas chromatography system a baseline separation of C10-C28 n-alkanes was achieved in less than 3 minutes.
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    Proper Tuning of Comprehensive Two-Dimensional Gas Chromatography (GC×GC) to Optimize the Separation of Complex Oil Fractions (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 182-188 
    Details
    ISSN: 0935-6304
    Keywords: Comprehensive two-dimensional gas chromatography ; GC×GC ; thermal modulation ; oil analysis ; petroleum analysis ; analysis of middle-distillate oil fractions ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---Comprehensive two-dimensional gas chromatography (GC×GC) is an utterly suitable separation technique for the analysis of complex samples, such as oil fractions. Once the two columns and the operating conditions are properly tuned, the technique is able to provide a detailed characterization of such materials. Some considerations applying to the tuning of a GC×GC system for a specific separation are presented and discussed. The authors present a number of different column sets and conditions which allow the separation of a non-aromatic hydrocarbon solvent, a kerosene, the light end of a crude oil, and an olefinic fraction, respectively. The highly structured GC×GC chromatograms, together with chemical knowledge about the samples, provide a much more comprehensive characterization of the samples than hitherto possible.
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    Comprehensive Two-Dimensional Gas Chromatography with a Rotating Thermal Desorption Modulator and Independently Temperature-Programmable Columns (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 189-196 
    Details
    ISSN: 0935-6304
    Keywords: Comprehensive two-dimensional gas chromatography ; thermal desorption modulator ; temperature programming ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---In comprehensive two-dimensional gas chromatography, two individual separations are coupled by means of a rotating thermal desorption modulator interface. The injection pulse introduced via the interface onto the second column should be as short as possible. Parameters affecting the modulator operation are studied. In the set-up used in this study, the temperature of the second column can be programmed independently from that of the first column. Optimization of the second-dimension separation to minimize peak broadening and maximize resolution is discussed and an elegant approach to determine second-dimension retention times using a non-constant modulation frequency is demonstrated. The high separation power of the comprehensive system is demonstrated by the analysis of technical and biota samples containing chlorinated biphenyls and toxaphene.
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    Determination of Oxygenates in Gasoline by GC×GC (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 197-201 
    Details
    ISSN: 0935-6304
    Keywords: Multidimensional gas chromatography ; comprehensive two-dimensional gas chromatography ; GC×GC ; oxygenates ; MTBE ; reformulated gasoline ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---Comprehensive two-dimensional gas chromatography (GC×GC) has been applied to the quantitation of oxygenates in reformulated gasoline. Target oxygenates were C1-C4 alcohols, tert-pentanol, methyl tert-butyl ether (MTBE), diisopropyl ether (DIPE), ethyl tert-butyl ether (ETBE), and tert-amyl methyl ether (TAME). These were separated from the gasoline matrix using a volatility-based selectivity in the first chromatographic dimension, followed by a mixed-phase polarity/shape selectivity in the second dimension. The high resolving power of this stationary phase combination completely separated all oxygenates except DIPE, ETBE, and TAME, which exhibited coelution with other nonpolar gasoline components. Oxygenates quantitation was achieved with the use of an internal standard, an FID detector, and calibration curves. Quantitation results are in good agreement with ASTM and EPA standard methods. When coupled with our previous method for BTEX and aromatics, a single GC×GC method can now quantitate MTBE, alcohols, BTEX, and aromatics in a one-hour analysis.
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    Biodegradation of Pharmaceutical Residues Investigated by SPE-GC/ITD-MS and On-Line Derivatization (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 474-478 
    Details
    ISSN: 0935-6304
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---Solid-phase extraction, on-line derivatization, and measurement by ion trap mass spectrometry (ITD-MS) were used to investigate the biological degradation of pharmaceutical residues (clofibric acid, ibuprofen, diclofenac). The results of the single steps of sample pretreatment and analytical determination are reported. MS/MS measurements were performed on an ITD-MS by selecting collision induced dissociation of the molecular ions (M+) as parent ions to defined daughter ions. A pilot sewage plant and biofilm reactors operating under oxic and anoxic conditions were run as model systems with synthetic sewage water containing 10 to 50 mg/L dissolved organic carbon (DOC) and pharmaceuticals in concentrations of 10 μg/L. Clofibric acid displayed its persistent character in all cases. The pilot sewage plant and the oxic biofilm reactor showed comparable results for diclofenac and ibuprofen, which both were partly degraded. These results can explain the occurrence of these substances in sewage effluents and in the aquatic environment. A high degree of degradation was found especially for ibuprofen in the oxic biofilm reactor, which was attributed to adaptation of the biofilm to the residue. Two metabolites of ibuprofen could be identified on the basis of their mass spectra and comparison with literature data, viz. hydroxyibuprofen and carboxyibuprofen.
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    Characterization of Polydimethylsiloxanes Using an Off-Line Combination of Supercritical Fluid Chromatography with Matrix-Assisted Laser Desorption Ionization Mass Spectrometry (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 502-506 
    Details
    ISSN: 0935-6304
    Keywords: Silicone oil ; supercritical fluid chromatography (SFC) ; micropacked columns ; MALDI-TOF ; GC/MS ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---No abstract
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    Separation and Detection of Metal Ions in Ecological Samples by Capillary Zone Electrophoresis with Indirect UV Detection (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 511-514 
    Details
    ISSN: 0935-6304
    Keywords: CZE ; indirect UV detection ; metal ions ; ecological samples ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---No abstract
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    Solventless Sample Preparation Techniques in Environmental Analysis (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 297-303 
    Details
    ISSN: 0935-6304
    Keywords: Sample preparation ; gas extraction techniques ; membrane-type techniques ; water analysis ; soil analysis ; gas chromatography ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---This review focuses on the role of so-called solventless methods of sample preparation in contemporary environmental analysis. The basic classification of this group of methods as well as the principal features and advantages of the techniques in current use are discussed.
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    Comparison of Thermal Sweeper and Cryogenic Modulator Technology for Comprehensive Gas Chromatography (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 253-258 
    Details
    ISSN: 0935-6304
    Keywords: Comprehensive gas chromatography ; cryogenic modulation ; semi-volatile aromatics ; thermal sweeper ; peak widths ; peak asymmetries ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---The two current technologies for achieving comprehensive gas chromatography (GC×GC) - the thermal sweeper and the cryogenic modulator - are compared in an interlaboratory study using a multicomponent semi-volatile aromatic compound sample. The same column set (phases, film thickness, dimensions of columns) and conditions of oven temperature program were used. Carrier gas flow settings however were different for the data reported here. The thermal sweeper has a longer overall length due to the extra ca. 30 cm length of narrow bore tubing used for the modulator/accumulator section. Data reveal that the two methods behave in an analogous manner in respect of delivering GC×GC results, with key peak parameters of peak widths and symmetry measures showing good correlation. Retention time dissimilarity on the first dimension columns in the two systems arises from different flow rates used, however the second column retention is similar, and this is due to the resulting different elution temperatures that peaks elute on the first dimension in each system. Overall, the two approaches to GC×GC appear to produce equivalent results within the scope of the application studied. Each system does have its experimental limitations; the thermal sweeper has what may be called a ‘thick film effect’, where at high temperature it can be difficult to sufficiently trap the migrating bands in the accumulator column, and the pulsing of solutes in the cryogenic system may suffer from a ‘thick wall effect’ if a column with too thick a wall dimension is used at low oven temperature.
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    Is Multidimensional High Performance Liquid Chromatography (HPLC) an Alternative in Protein Analysis to 2D Gel Electrophoresis? (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 259-265 
    Details
    ISSN: 0935-6304
    Keywords: Proteomics ; protein analysis ; multidimensional HPLC ; ion-exchange chromatography ; reversed phase chromatography ; comprehensive HPLC ; two-dimensional HPLC ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---The interactive modes of High Performance Liquid Chromatography (HPLC) of proteins provide a platform for the construction of a multidimensional HPLC system coupled to mass spectrometry. We present a system composed of both anion and cation exchanger columns, in the first dimension, and n-octadecyl bonded 1.5 μm nonporous silica columns in the second dimension. Both columns are operated under gradient conditions. A system suitability test with standard proteins showed that the total analysis can be performed within about 20 minutes. The fractions taken from the ion exchanger column are directly analyzed within one minute on the reversed phase column at a high flow rate. Two reversed phase columns are applied and operated alternatively: while the first column performs the separation within one minute, the analytes leaving the first dimension are enriched in an on-column focusing mode on top of the second column. The sample clean-up and enrichment is performed on a novel type of restricted access cation exchanger column with internal sulfonic acid groups and external diol groups. The columns exhibit a molecular weight exclusion limit for globular proteins of about 15 kDa. Our next studies will be directed towards the analysis of proteins and peptides from extracts of fibroblasts.
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    Fiber Conditioners for Solid Phase Microextraction: Design, Testing, and Application (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 343-347 
    Details
    ISSN: 0935-6304
    Keywords: SPME ; desorption ; fiber conditioning ; field sampling ; sample preparation ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: No abstract
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    Silver Ion High-Performance Liquid Chromatographic Separation of Conjugated Linoleic Acid Isomers, and other Fatty Acids, after Conversion to p-Methoxyphenacyl Derivatives (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 348-352 
    Details
    ISSN: 0935-6304
    Keywords: Silver ion high-performance liquid chromatography (Ag-HPLC) ; conjugated linoleic acid (CLA) ; p-methoxyphenacyl derivatives ; single-column Ag-HPLC ; fatty acid analysis ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---No abstract
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    Seed Oil Fatty Acids of Mongolian Compositae: The trans-Fatty Acids of Heteropappus hispidus, Asterothamnus centrali-asiaticus and Artemisia palustris (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 360-366 
    Details
    ISSN: 0935-6304
    Keywords: Artemisia palustris ; Asterothamnus centrali-asiaticus ; capillary gas liquid chromatography ; Compositae (Asteraceae) ; Heteropappus hispidus ; seed oil ; silver ion thin layer chromatography ; trans-fatty acids ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---Seed oils from the Compositae plant family are known to contain a variety of unusual fatty acids. Subsequent to the recent discovery of γ-linolenic acid in Saussurea and Youngia, further Mongolian Compositae species were investigated for their seed oil fatty acid composition. A number of δ3trans-fatty acids (16 : 1δ3t, 18 : 1δ3t and 18 : 3δ3t, 9c, 12c) were found in the seed oils of Heteropappus hispidus and Asterothamnus centrali-asiaticus. The latter fatty acid, but not the trans-monoenes, was also found in one species of Artemisia. These unusual fatty acid isomers were characterized by capillary gas-liquid chromatographic (GLC) separations in combination with other chromatographic techniques (analytical thin layer chromatography, TLC and preparative argentation TLC), and infrared spectrocsopy (IR). Their identity was further confirmed by co-chromatography with other seed oils known to contain these trans-fatty acids. The fact that within the Compositae plant family there are apparently two or three distinct groups of genera containing δ3trans-fatty acids is discussed.
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    2H/1H Ratio Analysis of Flavor Compounds by On-Line Gas Chromatography Pyrolysis Isotope Ratio Mass Spectrometry (HRGC-P-IRMS): Benzaldehyde (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 357-359 
    Details
    ISSN: 0935-6304
    Keywords: 2H/1H ratio analysis ; HRGC-P-IRMS ; stable isotopes ; benzaldehyde ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---Isotope ratio monitoring gas chromatography-mass spectrometry of the 2H/1H ratio by pyrolysis isotope ratio mass spectrometry (P-IRMS) was used to analyze benzaldehyde originating from various sources. Based on the δ2HSMOW value of an authentic reference sample determined with an elemental analyzer (EA), the range of reproducibility and linearity was checked. Correct (EA related) and reproducible data were obtained for sample amounts 〉0.6 μg benzaldehyde (on column). In another series of experiments, the influence of sample preparation, i. e. simultaneous distillation-extraction (SDE) was found to be negligible. The following ranges of δ2HSMOW values were determined for benzaldehyde using five types of samples, i. e. (i) synthetic (δ2HSMOW -78 to -85‰, ex benzal chloride; +420 to +668‰, ex toluene) and ‘natural’ (including ‘ex-cassia’) references (δ2HSMOW -83 to -144‰); (ii) bitter almond oils (δ2HSMOW -113 to -148‰); (iii) fruits (δ2HSMOW -111 to -146‰); (iv) kernels (δ2HSMOW -115 to -188‰); and (v) leaves (δ2HSMOW -165 to -189‰).
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    Effects of Injected Volume and Applied Voltage on Column Efficiency in Capillary Electrochromatography with Open Tubular Columns of 10 μm i. d. (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 373-378 
    Details
    ISSN: 0935-6304
    Keywords: Open tubular columns ; capillary electrochromatography ; column efficiency ; injected volume ; applied voltage ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The effect of some instrumental parameters on column efficiency in open tubular capillary electrochromatography (OTCEC) has been evaluated. First, it was investigated whether band broadening due to the sample injection process is within a tolerable range when an open tubular column (OTC) of about 10 μm i. d. is used. As a result of the small injection profile factor (K2 = 1.3), injected volumes must be sufficiently small (less than 10 pL) to avoid a significant efficiency loss (〉5%) when hydrodynamic injection by siphoning is employed. Secondly, the kinetic performance of OTCs in a CEC system was estimated from the variation of the reduced plate height (h) with the reduced linear velocity (ν) which was controlled by the voltage applied. Reasonable agreement was obtained between the theoretical h versus ν curve and the experimental values for a group of polycyclic aromatic hydrocarbons used as test compounds. Values of 0.25 for minimum h at an optimum ν of 16 are estimated, which permit separations with around 400,000 plates per meter to be obtained in less than 5 min. Finally, the possibility of estimating the diffusion coefficients of the solutes in the mobile phase from the plot of the height of a theoretical plate versus electroosmotic flow velocity is shown.
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  • 97
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    Electronic Resource
    Separation of Enantiomers of Drugs by Capillary Electrophoresis with Permethyl-gamma-Cyclodextrin as Chiral Solvating Agent (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 413-429 
    Details
    ISSN: 0935-6304
    Keywords: Capillary electrophoresis ; enantiomer separation ; chiral drugs ; TM-γ-cyclodextrin ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---High-throughput screening is a promising new approach in analytical chemistry. Within the framework of an extended screening program (The German-Chinese Drug Screening Program), the enantioseparation of 86 drugs was investigated by capillary zone electrophoresis in the presence of the chiral solvating agent (CSA) octakis-(2,3,6-tri-O-methyl)-γ-cyclodextrin (TM-γ-CD). By this means, 15 drugs could be separated into enantiomeric pairs. Approximate measures for the degree of interaction (migration retardation factor, Rm) and for the degree of enantiomer recognition (migration separation factors, αm) revealed intriguing patterns that were compared with those found for native γ-cyclodextrin (γ-CD). Although there is a distinct influence of the analyte structure on the electrophoretic data, interpretation remains difficult. Most remarkably, permethylation of γ-CD leads neither to a higher affinity nor to better chiral recognition, in contrast to the findings with α-CD.
    Additional Material: 10 Ill.
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  • 98
    Electronic Resource
    Electronic Resource
    Retention Prediction System of O-Aryl,O-(1-methylthioethylidene-amino)phosphates on RP-HPLC (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 445-448 
    Details
    ISSN: 0935-6304
    Keywords: O-Aryl,O-(1-methylthioethylideneamino)phosphates ; retention prediction ; quantitative structure-retention relationship (QSRR) ; reversed-phase HPLC ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---Using factor analysis and stepwise linear regression methods, two parameters - CMR and ECCR - were selected from eight solute-related structure parameters as the most retention-influencing parameters. The relationships between the retention data (k ´) and the two structure parameters were established for 13 O-aryl,O-(1-methylthioethylideneamino)phosphate compounds under a wide range of experimental conditions. The retention data (k ´) of another seven compounds with similar structures were predicted using these QSRR equations. Good agreement was obtained between the experimental k ´ values and predicted ones.
    Additional Material: 1 Ill.
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  • 99
    Electronic Resource
    Electronic Resource
    Temperature-Programmed Packed Capillary Liquid Chromatography Coupled to Fourier-Transform Infrared Spectroscopy (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 525-530 
    Details
    ISSN: 0935-6304
    Keywords: Packed capillary liquid chromatography ; temperature programming ; Fourier-transform infrared spectroscopy ; polymer additives ; pneumatic nebulization ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---Temperature-programmed packed capillary liquid chromatography has been coupled off-line to Fourier-transform infrared spectroscopy, utilizing a commercially available interface with a pneumatic nebulizer rebuilt to handle low flow rates at elevated temperatures. The modified interface showed excellent performance with regard to non-aqueous reversed phase separations of polymer additives, resulting in constructed Gram-Schmidt chromatograms comparable to chromatograms obtained using UV detection. The spray of the in-house constructed nebulizer was not influenced by temperature changes of the column effluent, and hence temperature-programmed gradient separations could be used successfully. The relative standard deviation of peak height was 4.4% (n = 5) and the mass limit of detection was determined to be about 40 ng, using a polymer antioxidant as model compound. The present instrumental coupling has been used for characterization of the antioxidant Irgafos P-EPQ.
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  • 100
    Electronic Resource
    Electronic Resource
    Separation of Enantiomers by On-Line Capillary Isotachophoresis-Capillary Zone Electrophoresis (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 531-538 
    Details
    ISSN: 0935-6304
    Keywords: Capillary isotachophoresis ; capillary electrophoresis ; column-coupling electrophoresis ; enantiomers ; chiral ; amino acids ; tryptophan ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---The ability of capillary zone electrophoresis (CZE) coupled on-line with capillary isotachophoresis (ITP) sample pretreatment in the column-coupling capillary electrophoresis equipment to separate trace enantiomers present in samples of complex ionic matrices and enantiomers present in their mixtures at significantly differing concentrations has been studied. Enantiomers of 2,4-dinitrophenyl labeled norleucine (DNP-Nleu) and tryptophan enantiomers were employed as model analytes in this work while urine and mixtures of tryptophan enantiomers of differing concentrations served as model samples. Experiments performed with urine samples spiked with the DNP-Nleu racemate at sub-μmol/L concentrations demonstrated excellent sample pretreatment capabilities of ITP (concentration of the analytes, in-column and post-column sample clean up) when coupled on-line with chiral CZE separations. In the CZE separations of enantiomers present in the samples at trace concentrations the sample pretreatment could be performed in both achiral and chiral ITP electrolyte systems. The use of a chiral electrolyte system was found to be essential in the ITP pretreatment of the samples containing the enantiomers at very differing concentrations. For example, a 2×10-7 mol/L concentration of L-tryptophan could be detected in the CZE separation stage of the ITP-CZE combination in samples containing about a 104 excess of D-tryptophan only when the ITP pretreatment was carried out in the electrolyte system providing the resolution of enantiomers (α-cyclodextrin served for this purpose in the present work). A post-column ITP sample clean up was found effective in enhancing the destacking rate of the trace enantiomer in the CZE stage when the migration configuration of the enantiomers was less favorable (the trace constituent migrating behind the major enantiomer).
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