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  • Column liquid chromatography  (119)
  • stability  (54)
  • Coleoptera  (44)
  • Springer  (217)
  • Institute of Physics
  • 1995-1999
  • 1990-1994  (217)
  • 1940-1944
  • 1993  (217)
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Keywords
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  • 1995-1999
  • 1990-1994  (217)
  • 1940-1944
Year
  • 1
    Electronic Resource
    Electronic Resource
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    Computing 51 (1993), S. 271-292 
    ISSN: 1436-5057
    Keywords: 65N15 ; 65N99 ; 35A40 ; Box method ; boundary value problem ; finite volume method ; variational formulation ; stability ; error bounds
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Description / Table of Contents: Zusammenfassung Box-Methoden (Finite-Volumen-Methoden) sind verbreitete Verfahren zur Lösung physikalischer Erhaltungsgleichungen, insbesondere in der Strömungsmechanik. In dieser Arbeit werden zwei Methoden für elliptische Differentialgleichungen untersucht, die Diagonal-Boxen und die Schwerpunkt-Boxen. Da die Box-Methoden im Sinne von Petrov-Galerkin-Verfahren interpretiert werden können, erhält man vergleichbar zur Finiten-Element-Methode eine variationsrechnerische Stabilitäts- und Fehleranalyse. Damit werdenO(h)- undO(h 2)-Fehlerabschätzungen hergeleitet. Lokale Eigenwertprobleme führen zu Stabilitätsaussagen. Allerdings ergibt sich eine Abhängigkeit von der Anzahl und Art gestörter Vierecke. Insbesondere die Diagonal-Boxen sind anfällig für lokale Störungen.
    Notes: Abstract Box schemes (finite volume methods) are widely used in fluiddynamics, especially for the solution of conservation laws. In this paper two box-schemes for elliptic equations are analysed with respect to quadrilateral meshes. Using a variational formulation, we gain stability theorems for two different box methods, namely the so-called diagonal boxes and the centre boxes. The analysis is based on an elementwise eigenvalue problem. Stability can only be guaranteed under additional assumptions on the geometry of the quadrilaterals. For the diagonal boxes unsuitable elements can lead to global instabilities. The centre boxes are more robust and differ not so much from the finite element approach. In the stable case, convergence results up to second order are proved with well-known techniques.
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  • 2
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    Mathematical programming 61 (1993), S. 197-214 
    ISSN: 1436-4646
    Keywords: Epi-convergence ; epi-distance ; stability ; convex optimization ; approximate solutions ; subgradients ; level sets
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science , Mathematics
    Notes: Abstract We prove that theε-optimal solutions of convex optimization problems are Lipschitz continuous with respect to data perturbations when these are measured in terms of the epi-distance. A similar property is obtained for the distance between the level sets of extended real valued functions. We also show that these properties imply that theε-subgradient mapping is Lipschitz continuous.
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  • 3
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    Entomologia experimentalis et applicata 69 (1993), S. 13-20 
    ISSN: 1570-7458
    Keywords: Artificial diets ; Coccinellidae ; rearing ; biological control ; Chilocorus spp. ; Coleoptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Promising diets were screened and the most successful modified with additives used in artificial diets for other entomophagous insects. Two suitable diets were obtained, one for adults and one for larvae ofChilocorus nigritus (Fabricius) (Coleoptera: Coccinellidae). They were still inferior to natural prey and not adequate as the sole food source for rearing consecutive generations. They are valuable as substitute food in the insectary during shortages of natural prey. Oleander scaleAspidiotus nerii Bouché andAsterolecanium miliaris (Boisduval) were evaluated as natural prey forC. nigritus and two other potential biocontrol agents in southern Africa,C. bipustulatus (Linnaeus) andC. infernalis Mulsant.A. nerii andA. miliaris were suitable for all life stages ofC. nigritus and adults ofC. bipustulatus andC. infernalis. A. miliaris was inadequate for larvae ofC. bipustulatus andC. infernalis.
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  • 4
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    Entomologia experimentalis et applicata 66 (1993), S. 161-169 
    ISSN: 1570-7458
    Keywords: avoidance behaviour ; capture efficiency ; Coleoptera ; time-lapse video ; visual recording
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Pitfall trap capture forSitophilus oryzae (L.),S. zeamais (Motschulsky),S. granarius (L.),Tribolium confusum (Duval),T. castaneum (Herbst);Oryzaephilus surinamensis (L.),Cryptolestes pusillus (Schonherr),Rhyzopertha dominica (Fabr.), andProstephanus truncatus (Horn) in millet was assessed by visual and time-lapse video recordings. The behaviour of different beetle species in arenas containing millet was monitored over 24 h and the frequency of encounters with the trap rim resulting in capture recorded. The capture efficiency of four types of pitfall traps (i.e. polystyrene, polythene, glass and tin-plated steel can) with rims exposed or submerged below the millet surface level were compared. Capture was related to beetle size, locomotory rate, and beetle behaviour at the trap rim as well as trap design and placement. The lighter and smaller species were least captured. Glass jars were more effective than plastic and metal containers. Traps placed with their rims submerged below the grain surface level were more efficient than those with rims exposed. Capture rate was unrelated to trap size. The frequency of encounters with trap rims was not correlated with capture rate. Three types of avoidance behaviours at the trap rims i.e. probing, skirting and spontaneous retreat, were related to capture rate, spontaneous retreat being the most effective escape mechanism and probing least.
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  • 5
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    Entomologia experimentalis et applicata 66 (1993), S. 191-196 
    ISSN: 1570-7458
    Keywords: Leptinotarsa decemlineata (Say) ; Coleoptera ; Chrysomelidae ; distribution ; diapause ; overwintering ; mortality
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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  • 6
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    Journal of intelligent manufacturing 4 (1993), S. 183-198 
    ISSN: 1572-8145
    Keywords: Assembly planning ; subassembly ; liaison graph ; geometric reasoning ; assembly cost ; stability ; partial-order graph
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: The distribution of assembly workstations enables assembly operations to be done in parallel, while the multiple routing of parts in flexible assembly systems allows the opportunistic scheduling of assembly operations. This paper presents an assembly planning system, called the Assembly Coplanner, which automatically constructs an assembly partial order and generates a set of assembly instructions from a liaison graph representation of an assembly based on the extraction of preferred subassemblies. Assembly planning in Coplanner is carried out by the co-operation of multiple planning agents, such as the geometric reasoner, the physical reasoner, the resource manager and the plan coordinator, under the constraints of finding a cost-effective assembly plan in a flexible assembly system. The Coplanner identifies spatial parallelism in assembly as a means of constructing temporal parallelism among assembly operations. This is achieved in the following way: (1) the selection of a set of tentative subassemblies by decomposing a liaison graph into a set of subgraphs based on feasibility and difficulty of disassembly; (2) the evaluation of each of the tentative subassemblies in terms of assembly cost represented by subassembly selection indices; and (3) the construction of a hierarchical partial order graph (HPOG) as an assembly plan. A case study applying the Coplanner to a mechanical assembly is illustrated in this paper.
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  • 7
    ISSN: 1572-8900
    Keywords: Chemiluminescence ; oxidation ; stability ; acrylonitrile-butadiene-styrene (ABS) ; ABS/polycarbonate blend
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The thermal oxidative stability of various ABS/PC compounds was studied by means of the chemiluminescence technique. Similarly to pure ABS, Irganox 1076 and Irganox MD 1024 perform as moderate antioxidants in ABS/PC and (ABS/PC + lubricant) blends. Neither Tinuvin 144, Irgaphos 168, nor their mixture affects the durability of the ABS/PC blend. At the same time, (Irgaphos 168 + Tinuvin 144) in combination with Irganoxes was found to provide a noticeable enhancement in durability to the (ABS/PC + lubricant) system. Titanium dioxide pigments by themselves have only a slight influence on the oxidative stability of the ABS/PC blend. Durability of the (ABS/PC + pigment) and (ABS/PC + lubricant) systems was found to be the same and the overall protective effect of Irganox 1076 was similar in both the (ABS/PC + lubricant) and the (ABS/PC + lubricant + pigment) systems. Certain modifiers significantly improve the durability of the ABS/PC compounds, although their function may differ in the systems with and without pigments.
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  • 8
    ISSN: 1572-8900
    Keywords: Chemiluminescence ; oxidation ; stability ; acrylonitrile-butadiene-styrene (ABS)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Energy, Environment Protection, Nuclear Power Engineering , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The influence of lubricants, UV stabilizers, antioxidants, and metal deactivators on the resistance of ABS to thermal oxidation was studied by means of the chemiluminescence technique. Neither of the additives seems to affect significantly the induction period of oxidation. At the same time, the influence of various additives on the oxidation rate constant is remarkably different: the introduction of lubricants and UV stabilizers increases its value, while antioxidants and metal deactivators have the opposite effect. For the particular systems studied durability is decreased in samples containing the lubricant and UV stabilizers and increased in samples stabilized with the antioxidant and metal deactivator.
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  • 9
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    Queueing systems 13 (1993), S. 87-110 
    ISSN: 1572-9443
    Keywords: Manufacturing systems ; semiconductor manufacturing ; thin film lines ; re-entrant lines ; scheduling policies ; queueing networks ; buffer priority policies ; due date policies ; stability ; stochastic control ; mean delay ; variance of delay ; machine failures ; set-up times
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Notes: Abstract Traditionally, manufacturing systems have mainly been treated as either job shops or flow shops. In job shops, parts may arrive with random routes, with each route having a low volume. In flow shops, the routes are fixed and acyclic, as in assembly lines. With the advent of semiconductor manufacturing plants, and more recently, thin film lines, this dichotomy needs to be expanded to consider another class of systems, which we call “re-entrant lines”. The distinguishing feature of these manufacturing systems is that parts visit some machines more than once at different stages of processing. Scheduling problems arise because several parts at different stages of processing may be in contention with each other for service at the same machine. There may be uncertainties in the form of random service or set-up times, as well as random machine failures and repairs. The goal of scheduling is to improve performance measures such as mean sojourn time in the system, which is also known as the mean “cycle-time”, or the variance of the cycle-time. In this paper we provide a tutorial account of some recent results in this field. We describe several scheduling policies of interest, and provide some results concerning their stability and performance. Several open problems are suggested.
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  • 10
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    Celestial mechanics and dynamical astronomy 56 (1993), S. 45-50 
    ISSN: 1572-9478
    Keywords: Restricted problem ; stability ; planets of double stars
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract Numerical simulations are made within the framework of the plane restricted three-body problem, in order to find out if stable orbits for planets around one of the two components in double stars can exist. For any given set of initial parameters (the mass ratio of the two stars and the eccentricity of their orbit around each other), the phase-space of initial positions and velocities is systematically explored. In previous works, systematic exploration of the circular model as well as studies of more realistic (elliptic) cases such as Sun-Jupiter and the nearby α Centauri and Sirius systems, large stable planetary orbits were found to exist around both components of the binary, up to distances from each star of the order or more than half the binary's periastron separation. The first results presented here for the η Coronae Borealis system confirm the previous studies.
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  • 11
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    Journal of dynamics and differential equations 5 (1993), S. 625-671 
    ISSN: 1572-9222
    Keywords: Scalar reaction-diffusion equation ; singular perturbation methods ; internal layer ; Neumann layer ; stability ; 35K57 ; 35B25 ; 35B35
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract The multiple existences and their stability properties of stationary solutions with a single transition layer in some scalar reaction-diffusion equation are shown. Each solution is constructed by using classical singular perturbation methods and its stability property is determined by a simple algebraic quantity, say index, appearing in the construction of a solution.
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  • 12
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    Queueing systems 14 (1993), S. 159-175 
    ISSN: 1572-9443
    Keywords: Queueing networks ; nonproduct form networks ; stability ; stochastic continuity ; functional limit theorems
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Notes: Abstract A finite number of nodes, each with a single server and infinite buffers, is considered in discrete time. The service may be FIFO and the service times are constant. The external arrivals and the routing decision variables form a general stationary sequence. Stability of the system is proved under these assumptions. Extension to multiple servers at a node and general stationary distributions holds. If the external input is i.i.d. and the routing is Markovian then stochastic ordering, continuity of stationary distributions, rates of convergence, a functional CLT and a functional LIL and various other limit theorems for the queue length process are also proved. Generalizations to multiple servers at nodes, customers with priority, multiple customer classes, general service length and Markov modulated external arrival cases are discussed.
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  • 13
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    Journal of dynamics and differential equations 5 (1993), S. 189-218 
    ISSN: 1572-9222
    Keywords: Equivariant bifurcation ; symmetry ; singularity ; equivariant jets and transversality ; normal forms ; universal unfolding ; stability ; structural stability ; 58F14 ; 58E07 ; 58C27
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract The theoretical machinery from singularity theory introduced by Golubitsky, Stewart, and Schaeffer, to study equivariant bifurcation problems, is completed and expanded while generalized to the multiple parameter context. In this setting the finite determinacy theorems or normal forms, the stability of equivariant bifurcation problems, and the structural stability of the universal unfolding are discussed.
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  • 14
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    Set-valued analysis 1 (1993), S. 393-402 
    ISSN: 1572-932X
    Keywords: Primary: 49J40, 65K10, 47A55, 47H05, 65L20 ; Variational inequality ; perturbation ; unbounded and nonsmooth operators ; convex sets ; Hausdorff distance ; regularization ; monotonicity ; convergence ; stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract The stability and convergence of the solutions of perturbed and regularized variational inequality to the solutions of the primary (unstable a priori) variational inequality with proper monotone operator are investigated. All the objects of inequality: the operatorA, the right-hand partf and the set of constrains Ω are to be perturbed. At the same time no assumptions of boundedness and smoothness of the operatorA are used. The connection between the parameters of perturbations, which guarantees strong convergence of approximate solutions, is established. It is proved that the existence of the solution to the unperturbed variational inequality is necessary and sufficient condition for convergence of the regularized perturbed inequality solutions.
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  • 15
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    Journal of dynamics and differential equations 5 (1993), S. 105-128 
    ISSN: 1572-9222
    Keywords: Delay system ; stability ; relative variance ; dynamical disease
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract A new approach to the study of delay systems, applicable to physiological control systems and other systems where little information about the time delays is available, is examined. The method is based on the fact that stability information can be deduced from the statistical properties of the probability distribution that encodes the structure of the time delay. The main statistical variables used are the usual expectation parameter,E, and a modified variance, calledrelative variance and denotedR, that is invariant under time scale changes. Recent work of the author has shown that stability often improves asR increases whileE remains fixed. A four-parameter family of delay models is analysed in this paper, and the (E, R) pair is found to be a reliable indicator of stability over the global parameter domain of the family.
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  • 16
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    Journal of solution chemistry 22 (1993), S. 1151-1158 
    ISSN: 1572-8927
    Keywords: Molecular complexes ; solvent effect ; stability ; solid CT complexes ; nicotine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Interaction of nicotine with tetracyanoethylene and iodine was investigated spectrophotometrically and found to form strong CT complexes (n−π and n-σ*, respectively). The donor site involved in CT interaction is the pyrrolidine nitrogen. The nicotine-I2 complex exists as the ionic structure (nicotine) I+-I 3 − . Formation constants of the CT complexes in various solvents were determined from 10 to 25°C and are discussed in terms of the nature of the electron acceptor and solvent polarity. Solid CT complexes were synthesized and were characterized by microchemical analysis and infrared spectra techniques.
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  • 17
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    Annals of global analysis and geometry 11 (1993), S. 387-395 
    ISSN: 1572-9060
    Keywords: Curvatures of hypersurfaces ; Reilly's inequality ; stability ; 53 A 10 ; 53 C 42
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract We generalize Reilly's inequality for the first eigenvalue of immersed submanifolds ofIR m +1 and the total (squared) mean curvature, to hypersurfaces ofIR m +1 and the first eigenvalue of the higher order curvatures. We apply this to stability problems. We also consider hypersurfaces in hyperbolic space.
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  • 18
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    BIT 33 (1993), S. 332-350 
    ISSN: 1572-9125
    Keywords: AMS(MOS): 65L06 ; Multistep collocation method ; continuous solution ; stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract This paper describes an implementation of multistep collocation methods, which are applicable to stiff differential problems, singular perturbation problems, and D.A.E.s of index 1 and 2. These methods generalize one-step implicit Runge-Kutta methods as well as multistep one-stage BDF methods. We give numerical comparisons of our code with two representative codes for these methods, RADAU5 and LSODE.
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  • 19
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    Entomologia experimentalis et applicata 68 (1993), S. 9-13 
    ISSN: 1570-7458
    Keywords: bioassay ; behavioural response ; Coleoptera ; carob extract ; stored product
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The behavioural responses ofCryptolestes pusillus Oryzaephilus surinamensis andProstephanus truncatus to different doses of carob extract were assessed in a two-choice pitfall bioassay and in plastic grain probe traps. The extract evoked a quick directional response and induced high beetle attraction to treated pitfall arenas compared with the controls. Overall, the extract improved probe trap efficiency by about 50% compared to unbaited probes. Beetle response was dose-dependent with the medium dose of 10 μl probably eliciting optimum response.
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  • 20
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    Annals of global analysis and geometry 11 (1993), S. 221-235 
    ISSN: 1572-9060
    Keywords: Second variation formula ; Morse index ; stability ; 53C
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract Iff is a minimal, isometric immersion of the Riemannian manifoldM of dimensionn in a Riemannian manifold $$\overline M$$ of dimensionm and ifI N is the „differential Jacobi operator” acting on the cross sections of the normal bundleN(M), we obtain some information on the Morse index and on the stability ofM through a detailed geometric analysis of the immersionf obtained when considering the higher fundamental forms off.
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  • 21
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    Celestial mechanics and dynamical astronomy 56 (1993), S. 323-324 
    ISSN: 1572-9478
    Keywords: chaos ; stability ; asteroids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
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  • 22
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    Celestial mechanics and dynamical astronomy 57 (1993), S. 59-94 
    ISSN: 1572-9478
    Keywords: asteroids ; libration ; proper elements ; stability ; chaos ; families
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract I have computed proper elements for 174 asteroids in the 1 : 1 resonance with Jupiter, that is for all the reliable orbits available (numbered and multi-opposition). The procedure requires numerical integration, under the perturbations by the four major planets, for 1,000,000 years; the output is digitally filtered and compressed into a “synthetic theory” (as defined within theLONGSTOP project). The proper modes of oscillation of the variables related to eccentricity, perihelion, inclination and node define proper elements. A third proper element is defined as the amplitude of the oscillation of the semimajor axis associated with the libration period; because of the strong nonlinearity of the problem, this component cannot be determined by a simple Fourier transform to the frequency domain. I therefore give another definition, which results in very good stability with time. For 87% of the computed orbits, the stability of the proper elements-at least over 1M yr-is within the following bounds: 0.001AU in semimajor axis, 0.0025 in eccentricity and sine of inclination. Half of the cases with degraded stability of the proper elements are found to be chaotic, with e-folding times between 16,000 and 660,000yr; in some other cases, chaotic behaviour does not result in a significantly decreased stability of the proper elements (stable chaos). The accuracy and stability of these proper elements is good enough to allow a search for asteroid families; however, the dynamical structure of the Trojan belt is very different from the one of the main belt, and collisional events among Trojans can result in a distribution of fragments difficult to identify. The occurrence of couples of Trojans with very close proper elements is proven not to be statistically significant in almost all cases. As the only exception, the couple 1583 Antilochus — 3801 Thrasimedes is significant; however, it is not easy to account for it by a conventional collisional theory. The Menelaus group is confirmed as a strong candidate collisional family; Teucer and Sarpedon could be considered as significant clusters. A number of other clumps are detected (by the same automated clustering method used for the main belt by Zappalà et al., 1990, 1992), but the total number of Trojans with reliable orbits is not large enough to detect many significant candidate families.
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    Celestial mechanics and dynamical astronomy 55 (1993), S. 249-259 
    ISSN: 1572-9478
    Keywords: Asteroid ; libration ; resonance ; stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract This paper investigates the stability of the motion in the averaged planar general three-body problem in the case of first-order resonance. The equations of the averaged motion of bodies near the resonance surface is obtained and is analytically integrated by quadratures. The stability of the averaged motion is analytically investigated in relation to the semi-major axes, the eccentricities and the resonance phases. An autonomous second-order equation is obtained for the deviation of semiaxes from the resonance surface. This equation has an energy integral and is analytically integrated by quadratures. The quasi-periodic dependence on time with two-frequency basis of the averaged motion of bodies is found. The basic frequencies are analytically calculated. With the help of the mean functionals calculated along integral curves of the averaged problem the new analytic first integrals are constructed with coefficients periodic in time. The analytic conditions of librations of resonance phases are obtained.
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  • 24
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    Celestial mechanics and dynamical astronomy 55 (1993), S. 323-330 
    ISSN: 1572-9478
    Keywords: Periodic orbits ; rigid body dynamics ; stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The equations of motion of a rigid body about a fixed point in a central Newtonian field is reduced to the equation of plane motion under the action of potential and gyroscopic forces, using the isothermal coordinates on the inertia ellipsoid. The construction of periodic solutions near the equilibrium points, by using the Lipaunov theorem of holomorphic integral, is obtained and the necessary and sufficient conditions for the stability of the system are given.
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    Celestial mechanics and dynamical astronomy 55 (1993), S. 351-367 
    ISSN: 1572-9478
    Keywords: Sitnikov's problem ; invariant rotational curves ; stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The Sitnikov's Problem is a Restricted Three-Body Problem of Celestial Mechanics depending on a parameter, the eccentricity,e. The Hamiltonian,H(z, v, t, e), does not depend ont ife=0 and we have an integrable system; ife is small the KAM Theory proves the existence of invariant rotational curves, IRC. For larger eccentricities, we show that there exist two complementary sequences of intervals of values ofe that accumulate to the maximum admissible value of the eccentricity, 1, and such that, for one of the sequences IRC around a fixed point persist. Moreover, they shrink to the planez=0 ase tends to 1.
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    BIT 33 (1993), S. 74-84 
    ISSN: 1572-9125
    Keywords: 65J10 ; 65M12 ; Analytic semigroup ; Banach space ; rational approximation ; A-acceptable ; A(θ)-acceptable ; stability ; Crank-Nicolson method
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract It is shown thatA-acceptable and, more generally,A(θ)-arational approximations of bounded analytic semigroups in Banach space are stable. The result applies, in particular, to the Crank-Nicolson method.
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    BIT 33 (1993), S. 285-303 
    ISSN: 1572-9125
    Keywords: 65L05 ; stiffness ; stability ; pseudospectra
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract It is argued that even for a linear system of ODEs with constant coefficients, stiffness cannot properly be characterized in terms of the eigenvalues of the Jacobian, because stiffness is a transient phenomenon whereas the significance of eigenvalues is asymptotic. Recent theory from the numerical solution of PDEs is adapted to show that a more appropriate characterization can be based upon pseudospectra instead of spectra. Numerical experiments with an adaptive ODE solver illustrate these findings.
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    Cellular and molecular life sciences 49 (1993), S. 171-174 
    ISSN: 1420-9071
    Keywords: Coleoptera ; Meloidae ; Epicauta funebris ; chemical defense ; biosynthesis ; terpenoid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Cantharidin, a potent defensive chemical, is present in all ten life stages of the blister beetleEpicauta funebris. The first five larval stages accumulate cantharidin as they feed and grow in size. When disturbed, they exude cantharidin in a milky oral fluid, not in hemolymph which adult beetles reflexively discharge from leg joints. Two subsequent larval stages and the pupa do not feed, grow, regurgitate, or change in their defensive reserves (110 μg cantharidin/insect, regardless of sex). Adult beetles kept in isolation for 60–90 d exhibit a pronounced sexual dimorphism in cantharidin production: the male biosynthesizes about 17 mg of the toxin, representing 10% of his live weight, whereas the female actually loses most of her defensive reserves. But in the wild a female beetle repeatedly acquires cantharidin as copulatory gifts from her mates.
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  • 29
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    Cellular and molecular life sciences 49 (1993), S. 272-275 
    ISSN: 1420-9071
    Keywords: Semiochemical ; pheromone ; host selection ; competition ; Coleoptera ; Scolytidae
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    Topics: Biology , Medicine
    Notes: Abstract Bark beetles,Ips typographus andPityogenes chalcographus, attracted by synthetic or natural pheromone to Norway spruce logs,Picea abies, preferred to colonize uninfested logs rather than logs occupied by these beetles, probably as a means of avoiding intra-and interspecific competition. The aggregation pheromone components ofP. chalcographus, chalcogran and methyl (E, Z)-2,4-decadienoate, inhibited the attraction response ofI. typographus to its pheromone components (methyl butenol andcis-verbenol), while the converse was not true. However, verbenone released from colonized bark inhibited pheromonal response ofP. chalcographus.
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  • 30
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    K-Theory 7 (1993), S. 333-367 
    ISSN: 1573-0514
    Keywords: Locally flat embedding ; pointed Hermitian module ; splitting ; stability
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    Topics: Mathematics
    Notes: Abstract We prove an existence result for topologically locally flat embeddings of 2-spheres in simply connected 4-manifolds. This topological result is deduced from a splitting theorem for pointed Hermitian modules over a cyclic group ring. A stability result for such modules is also proved. This applies to the isotopy classification of locally flat embeddings.
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  • 31
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    Multidimensional systems and signal processing 4 (1993), S. 91-101 
    ISSN: 1573-0824
    Keywords: Bilinear transformation ; polynomial ; stability
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    Topics: Electrical Engineering, Measurement and Control Technology
    Notes: Abstract The paper presents a unified approach to calculation, for a given, nominal,G-stable polynomial, a corresponding stability region in the space of perturbed coefficients
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  • 32
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    Multidimensional systems and signal processing 4 (1993), S. 331-354 
    ISSN: 1573-0824
    Keywords: two-dimensional discrete-time systems ; two-dimensional digital filters ; bivariate polynomials ; stability ; Bistritz tabular form ; Jury tabular form ; Schur-Cohn minors
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    Topics: Electrical Engineering, Measurement and Control Technology
    Notes: Abstract In determining root distribution of univariate polynomials with real or complex-valued coefficients, the Bistritz tabular form offers a significant computational advantage. Stability studies of two-dimensional (2-D) discrete-time systems involve univariate polynomials possessing parameter-dependent coefficients, where the parameter takes values on the unit circle in the complex plane. This paper investigates the application of Bistritz tabular form in determining stability of 2-D discrete-time systems, and for this purpose we present two algorithms. Both algorithms utilize a recent result that has established the relationship between Schur-Cohn minors and the entries of the Bistritz tabular form corresponding to a given polynomial. A comparison between the use of the modified Jury table and the Bistritz table in stability checking of 2-D discrete-time systems is also presented.
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  • 33
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    Journal of superconductivity 6 (1993), S. 55-57 
    ISSN: 1572-9605
    Keywords: Superconductivity ; Bi-2223 ; stability
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    Topics: Electrical Engineering, Measurement and Control Technology , Physics
    Notes: Abstract A general acid-base equilibrium theory was proposed to explain the formation and stability of the Bi-2223 phase based on the Lewis acid-base theory, and principle of metallurgical physical chemistry. The acid-base nature of oxide was defined according to the electrostatic force between cation and oxygen anion. A series of experimental facts were systematically explained based on the theory: substitution of Bi for Ca in. the Pb-free 2223 phase, and the effect of substitution of the high-valent cation for Bi3+; oxygen-pressure atmosphere, and the heat-schocking technique on the formation and stability of the 2223 phase.
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  • 34
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    Chemoecology 4 (1993), S. 29-32 
    ISSN: 1423-0445
    Keywords: chemical defence ; alkaloids ; predation ; Coleoptera ; Coccinellidae ; Adalia bipunctata ; Coccinella septempunctata ; Hymenoptera ; Formicidae ; Lasius niger
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Garden black ants,Lasius niger L., in a laboratory colony, attacked three species of live ladybirds found near their nest, killing the smaller two species. A second colony was offered artificial diets containing crushed ladybirds of two species, and the ants' choice of feeding site noted. Both the diets were aversive compared to control, but that containing 7spot,Coccinella septempunctata L., was more aversive than the diet containing 2spot,Adalia bipunctata L. The implications of this lesser protection for 2spots in terms of the chemical defence of the species are discussed.
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  • 35
    ISSN: 1423-0445
    Keywords: chemical defense ; predator-prey interaction ; synergism ; Coleoptera ; Staphylinidae ; Oxytelinae ; Bledius ; Carabidae ; Dyschirius ; Pogonus ; Dichirotrichus ; Formicinae ; Cataglyphis ; Dermaptera ; Labidura
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The adaptation of defensive secretions to their target organisms was examined for the abdominal gland secretions ofBledius furcatus, B. spectabilis andB. arenarius. Therefore the target organisms of the secretion of theseBledius species (i.e. their predators) had to be identified. At the collection sites examined these were the earwigLabidura riparia, the antCataglyphis bicolor, the flyLispe candicans, different carabids of the generaPogonus, Dichirotrichus, Dyschirius, Bembidion andCalathus and the wading birdsHaematopus ostralegus andCalidris alba. The secretion of the abdominal glands contains the toxin ptoluquinone dissolved in eitherγ-dodecalactone and 1-undecene (B. furcatus andB. spectabilis) or in octanoic acid and octyloctanoate (B. arenarius). The ratio of these solvents is species-specific. Application experiments using some of the natural insect predators (L. riparia, C. bicolor, Pogonus, Di. gustavii, Dyschirius) revealed that these solvent ratios provided a more effective deterrent than other possible ratios. Thus by combining the solvents in certain ratios, the capability of cuticular penetration and therefore the effectiveness of the defensive secretions are adapted to their natural targets.
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  • 36
    ISSN: 1432-1939
    Keywords: Coleoptera ; Herbivory ; Host selection ; Maternal effect ; Paternal effect
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    Topics: Biology
    Notes: Abstract The natural host of Ophraella notulata is Iva frutescens (Asteraceae); its close relative feeds on a related plant, Ambrosia artemisiifolia. We reared beetles on both plants, obtained progeny from the four possible crosses (two sexes X two parental hosts), and reared the progeny on both plant species. Survival to the imaginal stage of progeny reared on Iva varied with both maternal and paternal host. Hatchling feeding response to both plants showed a maternal host X paternal host interaction. Consumption of Ambrosia by adult beetles was, counter to expectation, higher for progeny of Iva-reared males than Ambrosia-reared males. Oviposition response, although based on too few data to be definitive, was peculiar: parental host did not affect oviposition on Ambrosia; on Iva daughters of Iva-reared males laid significantly more eggs than did daughters of Ambrosia-reared males, but only if they had been reared on Iva; those reared on Ambrosia displayed the reverse pattern. We discuss the possibility that nongenetic paternal transmission of host plant effects may explain these results, but offer a somewhat uncomfortable hypothesis of selection as a preferable explanation. An important outcome of the experiment is that it provided no evidence of maternal effects of host plant on offspring feeding or oviposition.
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  • 37
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    Journal of nonlinear science 3 (1993), S. 477-539 
    ISSN: 1432-1467
    Keywords: nearly integrable systems ; spectral transform ; attractors ; traveling waves ; stability ; numerical methods
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    Topics: Mathematics , Physics
    Notes: Summary In this paper we rigorously show the existence and smoothness inε of traveling wave solutions to a periodic Korteweg-deVries equation with a Kuramoto-Sivashinsky-type perturbation for sufficiently small values of the perturbation parameterε. The shape and the spectral transforms of these traveling waves are calculated perturbatively to first order. A linear stability theory using squared eigenfunction bases related to the spectral theory of the KdV equation is proposed and carried out numerically. Finally, the inverse spectral transform is used to study the transient and asymptotic stages of the dynamics of the solutions.
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  • 38
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    Journal of nonlinear science 3 (1993), S. 307-327 
    ISSN: 1432-1467
    Keywords: bifurcation ; saddle node ; Hopf ; stability ; robustness ; optimization ; numerical methods ; transcritical ; pitchfork ; extended systems
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics , Physics
    Notes: Summary Engineering and physical systems are often modeled as nonlinear differential equations with a vector λ of parameters and operated at a stable equilibrium. However, as the parameters λ vary from some nominal value λ0, the stability of the equilibrium can be lost in a saddle-node or Hopf bifurcation. The spatial relation in parameter space of λ0 to the critical set of parameters at which the stable equilibrium bifurcates determines the robustness of the system stability to parameter variations and is important in applications. We propose computing a parameter vector λ* at which the stable equilibrium bifurcates which is locally closest in parameter space to the nominal parameters λ0. Iterative and direct methods for computing these locally closest bifurcations are described. The methods are extensions of standard, one-parameter methods of computing bifurcations and are based on formulas for the normal vector to hypersurfaces of the bifurcation set. Conditions on the hypersurface curvature are given to ensure the local convergence of the iterative method and the regularity of solutions of the direct method. Formulas are derived for the curvature of the saddle node bifurcation set. The methods are extended to transcritical and pitchfork bifurcations and parametrized maps, and the sensitivity to λ0 of the distance to a closest bifurcation is derived. The application of the methods is illustrated by computing the proximity to the closest voltage collapse instability of a simple electric power system.
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  • 39
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    Acta applicandae mathematicae 32 (1993), S. 59-88 
    ISSN: 1572-9036
    Keywords: 47A56 ; 47A55 ; 15A54 ; 35A30 ; 34A30 ; Operator-valued functions ; matrices ; nonself-adjoint operators ; spectrum perturbation ; eigenvalues ; stability
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    Topics: Mathematics
    Notes: Abstract A survey is presented of estimates for a norm of matrix-valued and operator-valued functions obtained by the author. These estimates improve the Gel'fand-Shilov estimate for regular functions of matrices and Carleman's estimates for resolvents of matrices and compact operators. From the estimates for resolvents, the well-known result for spectrum perturbations of self-adjoint operators is extended to quasi-Hermitian operators. In addition, the classical Schur and Brown's inequalities for eigenvalues of matrices are improved. From estimates for the exponential function (semigroups), bounds for solution norms of nonlinear differential equations are derived. These bounds give the stability criteria which make it possible to avoid the construction of Lyapunov functions in appropriate situations.
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  • 40
    ISSN: 1573-1561
    Keywords: Encelia farinosa ; Asteraceae ; Trirhabda geminata ; Coleoptera ; Chrysomelidae ; feeding preferences ; acidic deposition ; plant stress
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Container grownEncelia farinosa were exposed to three 3-hr episodes of acidic fog (pH 2.5) typical of events in southern California. Adults and larvae of the specialist leaf-feeding herbivore,Trirhabda geminata, preferred to feed on the acidic-treated foliage compared to control fogged (pH 6.3–6.5) foliage. Previous feeding damage on the plants did not affect feeding preference. The acidic-fogged foliage was significantly higher in total nitrogen and soluble protein but not different from control-treated tissue in water content. Stress on native populations of this drought-deciduous shrub caused by atmospheric pollutants may also result in altered feeding ecology of the beetle.
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  • 41
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    Journal of chemical ecology 19 (1993), S. 395-410 
    ISSN: 1573-1561
    Keywords: Oreina gloriosa ; Coleoptera ; Chrysomelidae ; chemical defense ; cardenolides ; quantitative variation ; aging ; HPLC
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The defensive secretion of the alpine chrysomelidOreina gloriosa is a complex mixture of mainly cardenolides and tyrosine betaine. Individually sampled secretions of adult laboratory-reared and field-collected beetles were analyzed by reverse-phase HPLC; 16 secretion components were quantified. Quantities and concentrations of different components were significantly affected by the age, sex, and reproductive status of individual beetles. Aging was correlated with marked increases (up to 4.4-fold) and decreases (up to 2.7-fold) of quantities and concentrations of several components. Differences between the sexes were smaller, but quantities of all components and concentrations of several components were larger in laboratory-reared females than in males. There was less of one component of the secretion in mated than unmated females, but the concentrations of four secretion components were higher (up to 1.6-fold) in mated females.
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  • 42
    ISSN: 1573-1561
    Keywords: Rapeseed ; Brassica spp. ; allyl isothiocyanate ; glucosinolates ; allelochemicals ; Limonius californicus (Mann.) ; Coleoptera ; Elateridae ; toxicity ; sublethal effects
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Acute toxicity of soil amended with allyl isothiocyanate (AITC) to three size classes ofLimonius californicus (Mann.) wireworms was determined in the laboratory. Wireworms were exposed to AITC at initial concentrations of 120–300 nmol/g soil for one day. During this time, extractable AITC concentrations decreased by 66 to 93 %. Probit analysis estimated LC50 values of 238 and 226 nmol/g soil at one day posttreatment for medium and large wireworms, respectively. For small wireworms, LC50 values decreased from 211 to 157 nmol/g soil during 1–137 days posttreatment. Sublethal concentrations of AITC significantly reduced feeding activity of treated wireworms at three posttreatment times and over the entire 137 days. Wireworm weight was not significantly affected by AITC. The potential exists to use glucosinolate-containing plant tissue as an isothiocyanate (ITC) source to reduce crop damage caused byL. californicus wireworms.
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  • 43
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    Journal of chemical ecology 19 (1993), S. 1219-1231 
    ISSN: 1573-1561
    Keywords: Host recognition behavior ; entomopathogenic nematodes ; feces ; Spodoptera exigua ; Lepidoptera ; Noctuidae ; Popillia japonica ; Coleoptera ; Scarabaeidae ; Blattella germanica ; Blatteria ; BlattellidaeAcheata domesticus ; Orthoptera ; Gryllidae
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Host recognition by entomopathogenic nematodes may occur through contact with insects' excretory products, cuticle, or gut contents. We analyzed the behavioral responses of four species of entomopathogenic nematodes during contact with feces of natural or experimental hosts. Host recognition by nematodes was manifested in alterations in the frequency and/or duration of one or more search parameters including forward crawling, headwaving, body-waving, stopping, backward crawling, head-rubbing, and headthrusting.Heterorhabditis bacteriophora andSteinernema glaseri showed behavioral responses to contact with feces of their natural hosts,Spodoptera exigua (Lepidoptera) andPopillia japonica (Coleoptera), and to the experimental hosts,Acheata domesticus (Orthoptera) andBlatella germanica (Blatteria).Steinernema carpocapsae responded only toB. germanica feces, whereas5. scapterisci did not significantly respond to any of the insect species. During contact with cockroach feces, all nematodes, exceptS. scapterisci, showed avoidance behavior. We suggest that ammonia present in cockroach feces is inhibitory to nematodes. Specific host recognition by entomopathogenic nematodes may be an important mechanism to maintain host affinities.
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  • 44
    ISSN: 1573-1561
    Keywords: Holotrichia parallela ; large black chafer ; scarab beetle ; Coleoptera ; Scarabaeidae ; isoleucine methyl ester ; linalool ; sex pheromone ; circabidian periodicity ; pheromone titer
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract (R)-(−)-Linalool was identified as a minor component sex pheromone of the scarab beetleHolotrichia parallela (Coleoptera: Scarabaeidae). Field evaluations revealed that, although not attractive per se, (R)-(−)-linalool enhances the attractiveness of the major sex pheromone,L-isoleucine methyl ester (LIME). Analyses of the pheromone titers in the glands of field-collected females demonstrated the occurrence of peak levels of 48-hr (“circabidian”) periodicity. The levels of LIME in the glands of 45-day-old virgin females increased over three times from the scototo the photophase of a calling day, but the amounts of (R)-(−)-linalool did not significantly change. Virgin females had in average two times more LIME and 3.6 times more (R)-(−)-linalool than the average amount found in the field-captured beetles throughout the season.
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  • 45
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    Journal of chemical ecology 19 (1993), S. 1453-1459 
    ISSN: 1573-1561
    Keywords: Anomala schonfeldti ; Popillia japonica ; scarab beetle ; Coleoptera ; Scarabaeidae ; 2-(E)-nonenol ; sex pheromone ; mark-and-recapture ; field test ; mass trapping
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Synthetic 2-(E)-nonenol, previously identified as the sex pheromone ofAnomala schonfeldti (Coleoptera: Scarabaeidae), is demonstrated to be very attractive to males in the field. Nevertheless, no significant differences were found between treatments with 1, 5, 10, and 20 mg dosages. Males ofA. schonfeldti were more significantly attracted to traps at 30 cm high than at 90 cm. Although the observed behavior seemed to indicate a trend of more attraction to buried traps than those placed at 30 cm, there was no statistical difference between the two treatments. Pheromone-baited traps caught significantly more beetles than traps containing three virgin females. Over 70% of released beetles were recaptured in six traps surrounding the point of release and separated from each other by 50 m, suggesting a possible use of the pheromone (in combination with floral compounds) in mass trapping.
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  • 46
    ISSN: 1573-1561
    Keywords: Coleoptera ; Staphylinidae ; Tenebrionidae ; larvae ; defensive glands ; quinone ; naphthoquinone ; taxonomy
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The abdominal glands of three bark-inhabiting larvae of generaBolitochara, Leptusa (Staphylinidae), andHypophloeus (Tenebrionidae) were studied chemically and morphologically. Behavior of the larvae indicated that secretion is emitted only after severe disturbance of the larvae. These mechanical contacts may also occur incidentally with coinhabiting nonpredatory arthropods when the beetle larvae move within small interstices under bark. Depending on the species, the secretions contained 1,4-benzoquinone derivatives and three 6-alkyl-naphthoquinones dissolved within various alkanes, alkenes, ethyl-, isopropyl-, and isoamylesters. More erratically distributed gland constituents also detected were acetophenone, benzyl propionate, and methyl hydroxybenzoate. In the laboratory, synthetic quinone-containing solutions simulating those found inLeptusa andBolitochara larvae acted as strong topical irritants and caused further damage to last-stageCalliphora vomitoria larvae if hydrocarbons or esters were used as solvents. The natural secretions ofHypophloeus versipellis elicited considerable mortality in two subcortical sciarid larvae cooccurring with tenebrionid larvae. Bioassay and secretion chemistry of the Staphylinidae/Tenebrionidae larval secretions indicated that they are typical defensive secretions that act topically. Morphological data characterized Bolitocharini larvae as possessing protuberant abdominal tergites supplied with an interiorly situated gland reservoir. After mechanical contact, the defensive secretion is topically applied to other arthropods by dabbing this tergal protuberance on targets. The defensive gland ofHypophloeus versipellis is unusual in possessing a movable reservoir opening situated at the anterior border of tergite IX. By this peculiar gland morphologyHypophloeus larvae are capable of shooting secretion droplets frontally from their slightly depressed dorsal abdominal surface without bending their abdominal tips dorsally. This seems an adaptation to the interstitial habitat of the larvae. The types of defensive glands and their phylogenetic value in Aleocharinae/Tenebrionidae larvae are discussed.
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  • 47
    ISSN: 1573-1561
    Keywords: Hylastes cunicularius ; Hylastes brunneus ; Hylastes opacus ; Dryocoetes autographus ; Hylobius abietis ; Hylobius pinastri ; α-pinene ; terpenes ; turpentine ; ethanol ; ground traps ; Coleoptera ; Scolytidae ; Curculionidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Responses of threeHylastes species,Dryocoetes autographus, and twoHylobius species to terpenes and ethanol were studied in field experiments on clear-cut forest sites in Sweden using baited ground traps.α-Pinene alone did not attract any of the six species. A terpene blend (spruce turpentine consisting mainly ofα-pinene,β-pinene, and 3-carene) attractedHylastes cunicularius, H. brunneus, andHylobius abietis in some experiments, but not in others. The attractiveness of ethanol also varied; the only species consistently attracted wasH. abietis. Baits containing both terpenes and ethanol, particularly the combination of spruce turpentine and ethanol, were attractive to all species exceptHylobius pinastri. InH. abietis, the terpene plus ethanol/ ethanol catch ratios increased during early summer. Seasonal differences in catch levels were observed inH. cunicularius andH. abietis. The addition ofα-pinene reduced the attractiveness of the combination of spruce turpentine and ethanol toH. cunicularius, H. opacus, andD. autographus. The differences in response to the volatiles between species are probably related to differences in reproductive behavior and host preferences.
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  • 48
    ISSN: 1573-5028
    Keywords: antinutritional factor ; pea lectin ; site-directed mutagenesis ; stability
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    Topics: Biology
    Notes: Abstract Legume lectins are considered to be antinutritional factors (ANF) in the animal feeding industry. Inactivation of ANF is an important element in processing of food. In our study on the stability ofPisum sativum L. lectin (PSL), a conserved hydrophobic amino acid (Val103) in a surface loop was replaced with alanine. The mutant lectin, PSL V103A, showed a decrease in unfolding temperature (T m ) by some 10 °C in comparison with wild-type (wt) PSL, and the denaturation energy (ΔH) is only about 55% of that of wt PSL. Replacement of an adjacent amino acid (Phe104) with alanine did not result in a significant difference in stability in comparison with wt PSL. Both mutations did not change the sugarbinding properties of the lectin, as compared with wt PSL and with PSL from pea seeds, at ambient temperatures. The double mutant, PSL V103A/F104A, was produced inEscherichia coli, but could not be isolated in an active (i.e. sugar-binding) form. Interestingly, the mutation in PSL V103A reversibly affected sugar-binding at 37 °C, as judged from haemagglutination assays. These results open the possibility of production of lectins that are activein planta at ambient temperatures, but are inactive and possibly non-toxic at 37 °C in the intestines of mammals.
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  • 49
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    Acta mechanica Sinica 9 (1993), S. 277-288 
    ISSN: 1614-3116
    Keywords: generalized hybrid/mixed model ; element formulation ; equilibrium ; orthogonality ; least energy fit ; convergence ; stability ; coordinate invariance ; distortion insensitivity ; accuracy
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    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Abstract By the modified three-field Hu-Washizu principle, this paper establishes a theoretical foundation and general convenient formulations to generate convergent stable generalized hybrid/mixed element (GH/ME) model which is invariant with respect to coordinate, insensitive to geometric distortion and suitable for improved stress computation. In the two proposed formulations, the stress equilibrium and orthogonality constraints are imposed through incompatible displacement and internal strain modes respectively. The proposed model by the general formulations in this paper is characterized by including assumed stress/strain, assumed stress, variable-node, singular, compatible and incompatible GH/ME models. When using regular meshes or the constant values of the isoparametric Jacobian Det in the assumed strain interpolation, the incompatible GH/ME model degenerates to the hybrid/mixed element model. Both general and concrete guidelines for the optimal selection of element shape functions are suggested. By means of the GH/ME theory in this paper, a family of new GH/ME can be and have been easily constructed. The software can also be developed conveniently because all the standard subroutines for the corresponding isoparametric displacement elements can be utilized directly.
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  • 50
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Bovine milk ; Multivariate optimization ; Glycerophospholipids ; Molecular species separation ; Chemometrics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The molecular species profile, representing the fatty acid combinations on the glycerol backbone, is the most fundamental description of an intact glycerophospholipid class and provides a key to understanding its functional characteristics. We have developed a powerful RP-HPLC method for the separation of molecular species, including so-called “critical pairs”, of natural mixtures of 1,2-diacyl-sn-glycero-3-phosphocholine 1,2-diacyl-sn-glycero-3-phosphoethanolamine from bovine milk and other natural sources. We have used a novel multivariate development and optimization strategy to locate the region of optimum separation conditions within a prechosen domain of the chromatographic parameter space.
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  • 51
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Open tubular columns ; Cross-linked polyacrylate stationary phase ; Photopolymerization ; Antracene derivatives
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    Topics: Chemistry and Pharmacology
    Notes: Summary Polyacrylate films containing a lauryl moiety were fabricated on about 12 μm I.D. fused silica capillaries by in-situ photopolymerization of acrylates. Relatively thick films up to 1 μm were obtained by the technique. The films were tested as retentive layers for reversed phase open tubular liquid chromatography (OTLC). Due to the presence of the incorporated lauryl moiety in the layer considerable retention could be achieved for polar compounds with aqueous mobile phases. The efficiency of the prepared columns approximates to the theoretical value. The polyacrylate films appear to be extremely stable even under strong basic conditions. The films swell considerably in the presence of nonpolar solvents resulting in more favourable retention and a significant improvement of the chromatographic performance.
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  • 52
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    Chromatographia 35 (1993), S. 627-630 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Fatty acid analysis ; Fish oils
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    Topics: Chemistry and Pharmacology
    Notes: Summary A chromatographic method has been developed for the determination of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in fish oil dietary supplements containing triglycerides rich in (n−3) polyunsaturated fatty acids (PUFAs). Following the ester saponification, free fatty acids were resolved by TLC into two fractions: i) a more mobile group of saturated and monounsaturated fatty acids and ii) a less mobile group containing PUFAs. The PUFA fraction was further analyzed by HPLC to determine the levels of EPA and DHA in the fish oil sample.
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  • 53
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Carbidopa and levodopa in plasma ; Electrochemical detection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A validated reversed-phase high-performance liquid chromatographic procedure employing electrochemical detection (LCEC) for the analysis of carbidopa and levodopa in human plasma is reported. The method is sensitive and specific with amperometric detection at a glassy carbon working electrode with Eapp=0.75 V vs. Ag/AgCl. The retention times of levodopa, internal standard, and carbidopa are 3.3, 4.5, and 9.7 minutes, respectively, with an overall chromatographic run time of 12.0 minutes. The peak height ratio versus plasma concentration is linear over the range of 5.0 to 500 ng/mL for each analyte and exhibits correlation coefficients of 0.9957 or better (n=9). The mean absolute recovery of carbidopa and levodopa using the described assay is 36.6 and 66.0%, respectively. The inter- and intra-day accuracy and precision are within 11.8% of the actual values for all concentrations. Also, due to the demonstrated instability of carbidopa and levodopa in plasma a procedure is provided to circumvent this. Blood collected in pre-treated Vacutainer tubes can be stored in an ice bath for up to 4 hours without any significant degradation, thereby providing a practical means for processing several clinical samples simultaneously.
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  • 54
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Fullerenes ; FullereneSep® ; Separation factor ; HPLC-particle beam mass spectrometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary High-performance liquid chromatographic separation of fullerene mixtures in the range C60 to C100 can be achieved using a novel stationary phase which rivals the performance of the more expensive ‘Pirkle-type’ columns currently employed.
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  • 55
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    Chromatographia 35 (1993), S. 675-678 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Ion chromatography ; Trace metal determination ; Sample contamination ; Human hair
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The dynamic ion-exchange chromatographic analysis of bivalent ions, such as Cu, Pb, Zn, Ni, Co and Mn in resistant organic matrices needs preliminary wet ashing with oxidizing acids (HNO3 and HClO4). Such treatment may lead to an increase in the amounts of metals present in the sample due to their release from containers or equipment used in an acidic environment and to metal impurities contained in the acids. The effect of these interferences in the determination of trace metals in human hair was studied.
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  • 56
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; GC/ECD detection ; PCBs, ortho and non-ortho ; Chromatographic separation ; Congener determination ; FTIR identification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary An analytical procedure for the individual determination of ortho and non-ortho PCB congeners in sediments, using high performance liquid chromatography (HPLC) preseparation and gas chromatography/ECD detection, is described. Gas chromatography/FTIR spectrometry (GC/FTIR) and gas chromatography/mass spectrometry (GC/MS) were employed for individual congener identification and determination. Sample extraction, clean-up of extract and selective elution procedures were optimized by using reference certified marine sediment samples. Recovery and precision were typically 83% and 16% respectively at 2 ng/g of total PCB content. The proposed procedure, tested by analyzing real sediment samples, showed a reproducibility better than 20% at 13 ng/g PCB level.
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  • 57
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Glutamine ; Pyroglutamic acid ; Amino acids formulations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The aminoacid glutamine in aqueous solution and in conditions of high temperature and long term storage is partly transformed into pyroglutamic acid which exhibits potential neurotoxic effects. Commercially available aminoacid mixtures supplemented with glutamine are heat-sterilized and some losses of glutamine and formation of pyroglutamic acid may occur. The aim of the work was to set up an easy and reliable HPLC method which allows the determination of pyroglutamic acid as a degradation product of glutamine. The column was a 5 μm Hypersil ODS (100×4.6 mm) and the mobile phase 100% 0.007 M phosphate buffer pH 3.5. Stability studies in different conditions of temperature and time of storage were performed on aminoacid mixture available in the commerce.
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  • 58
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Carbonyl compounds ; Luminarin hydrazides ; Fluorescence detection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Fluorogenic reagents (luminarin 3, luminarin 11 and luminarin 12), having a quinolizinocoumarin moiety as fluorophore and a carboxylic acid hydrazide function as reacting group, have been developed. These reagents were found to be highly sensitive fluorescence derivatization reagents for aldehydes and ketones in high-performance liquid chromatography. The reagents readily react with carbonyl compounds in aqueous sulphuric acid solution (0.1 M) at room temperature to produce the corresponding hydrazone derivatives, which can be separated on both reversed or normal-phase column. The structures of the derivatives were studied, together with their properties in reversed and normalphase chromatographic systems. UV absorbance, corrected fluorescence spectral data and quantum yields of luminarin 3, luminarin 11 and luminarin 12 are presented. The detection limits (signal to noise ratio=3) for aldehydes and ketones were in the sub-pmol range. Luminarin 3 was also applied to the determination of hydroxymethylfurfural (HMF) in orange juices and concentrates. The method for HMF involves the solid-liquid extraction of the juice by using a C-18 cartridge prior to derivatization and normal-phase separation of the derivative with fluorimetric detection at 387 nmex., 444 nm em. The calibration curve was linear for amounts of HMF ranging from 0.1 to 10 nmol. Intrarun relative standard deviation was 12.8% for 0.1 nmol and 2.6% for 1 nmol. Recovery studies indicated an average of 98.7±1.9% for juice concentrate and 99.8±3.2% for pasteurized juice.
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  • 59
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Method development ; Chemometrics ; Response criteria ; Analysis of drug purity
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    Topics: Chemistry and Pharmacology
    Notes: Summary Optimization procedures require adequate response criteria to assess the quality of each chromatogram obtained during the process. The objective of this paper is to evaluate the possibility of using different resolution functions, in the chromatographic separation of a drug and its impurities (particularly the impurity just eluted after the drug). This study shows the limits of some resolution expressions. The interest of the simple Δt criterion, the difference between retention times, is presented in this paper.
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  • 60
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    Chromatographia 36 (1993), S. 147-151 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Polarity ; Retinoids
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    Topics: Chemistry and Pharmacology
    Notes: Summary The retention of retinoids in reversed-phase liquid chromatography was studied using aqueous mobile phases of different composition (methanol 94–86% and acetonityrile 92–82%) at five temperatures (40–60 °C). With both organic modifiers the effect of the molecular structure increased as the water content and the polarity of the mobile phase increased. The temperature-dependence increased in the same manner with aqueous acetonitrile mobile phases. The π-π interactions between the retinoids and acetonitrile diminish when the water content of the mobile phase is increased, as happens also to the hydrophobic interactions with both organic modifiers. The net effect of these changes depends on the composition of the mobile phase. There was excellent correlation of retention with all polarity parameters studied(ϕ, P′, xe, xd, xn, E T N , δT, δ, δo and δd), when the calculations were made separately with methanol and acetonitrile. The volume fraction of the organic modifier, ϕ, was the only parameter describing the retention well in both organic modifiers simultaneously.
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  • 61
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Aromatic amines ; Arylbenzylamide methylthioether derivative ; SL 85.1016 ; Pharmacokinetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary An HPLC method has been developed for the determination of SL 85.1016, a new calcium antagonist arylbenzylamide methylthioether derivative. SL 85.1016 and the internal standard, SL 87.0210, are extracted from alkaline human plasma withn-hexane and back extracted into 0.05 M phosphate buffer (pH 2.5; 0.2 ml). Acetonitrile (50 μl) is added to the final aqueous extract in order to prevent absorption of the compounds of interest onto the walls of the glass tube; this solution then is partially processed by HPLC on a C18 column with UV detection (254 nm). The determination limit of the method is 2 ng.ml−1 of SL 85.1016 in human plasma; the response to the drug is linear in the range 2–200 ng.mg−1.
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  • 62
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    Chromatographia 36 (1993), S. 347-350 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Allium ; Derivatization ; S-alk(en)yl-L-cysteine sulfoxides ; Sulfur amino acids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Two pre-column derivatization procedures coupled with reversed phase HPLC have been compared for the analysis ofS-Alk(en)yl0L-cysteine sulfoxides in variousAllium species. In order to establish external standards some (+/-) sulfoxides were synthesized, using a new method to enhance asymmetric synthesis of the diastereoisomers. The first derivatization method is the formation ofo-phthaldialdehyde/tert.-butylthiol derivatives which can be analyzed using UV detection. The second, presently used for amino acid analysis, is the Waters Pico-Tag method, which employs phenylisothiocyanate as derivatization reagent. As the Pico-Tag method was found to be the most efficient for determination ofS-alk(en)yl-L-cysteine sulfoxides it was used to determine the alliin content of various samples of garlic.
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  • 63
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Chlortetracycline ; Reversed-phase silical gel ; C8 bonded phase ; Collaborative study
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary An established method for the analysis of chlortetracycline by liquid chromatography using octylsilylated silica gel as the stationary phase was examined in a multicentre study involving five laboratories and a total of six columns. Three chlortetracycline hydrochloride samples were analysed. The main component and the impurities were determined. An analysis of variance, treating each column as a different laboratory, showed an absence of consistent between-laboratory bias and the presence of a significant laboratory-sample interaction. The repeatability and the reproducibility of the method, expressed as relative standard deviations of the result of the determination of chlortetracycline hydrochloride, were calculated to be 0.9% and 1.1% respectively.
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  • 64
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    Chromatographia 37 (1993), S. 627-634 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; ODS phases ; NMR and FTIR characterization ; Stationary phase functionality
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Two spectroscopic methods, solid state NMR and diffuse reflectance FTIR, have been used in conjunction with liquid chromatographicretention data to identify and distinguish the functionality of octadecylsilica (ODS) stationary phases. NMR and FTIR spectra are indicative of various features of the different functionalities of the phases and this information can be used to elucidate the configuration of the organosilyl moiety in the ODS on the silica surface and explain why DSS phases have different molecular shape (mainly planarity) recognition capability.
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  • 65
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    Chromatographia 37 (1993), S. 423-428 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Polymer-modified silica ; Polypyrrole cloride ; Polypyrrole dodecylsulfate ; Small molecules ; Polyaromatic hydrocarbons
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    Topics: Chemistry and Pharmacology
    Notes: Summary Polypyrrole chloride and polypyrrole dodecylsulfate coated-silica packing materials have been synthesized. Reverse-phase chromtographuy was employed to characterise both packings. A series of test compounds with known properties was used as molecular probes. They included benzene and derivatives, basic drugs, and polyaromatic hydrocarbons. A commericial C18 column was also used for the purpose of comparison in some cases.
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  • 66
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Chiralcel OD ; N-Alkyl-N-methylanilineN-oxide enantiomers ; Chiral nitrogen centre ; Flavin-containing monooxygenase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A high-performance liquid chromatographic method for the resolution of the enantiomers of a series ofN-alkyl-N-methylanilineN-oxides is reported. The resolutions were achieved using a Chiralcel, OD chiral stationary-phase with a mobile-phase of hexane and ethanol in varying proportions. The chromatographic order of elution of the enantiomers ofN-ethyl-N-methylanilineN-oxide was determined to be (+)-(R) before (−)-(S).
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  • 67
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    Chromatographia 37 (1993), S. 539-542 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Sotolon ; Vin jaune ; Flor-sherry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Sotolon (4,5-dimethyl-3-hydroxy-2(5H)-furanone) is a key flavour compound in the french flor-sherry “Vin jaune”. This compound was determined quantitatively by extraction of 25 ml of wine on a XAD-4 resin, elution with diethyl ether, separation by HPLC on a Lichrospher 100 Diol column, elution with dichloromethane/hexane (60/40) and UV detection at 232 nm. The amount of sotolon in “Vin jaune” (120 to 268 μg/l) was related to the development of the yeast film over a period of 6 years. Only 6 to 51 μg/l were found in the “Vin de paille” which is made with overmaturated grapes of the same Savagnin vine-plant but without development of yeasts, and 80 to 140 μg/l in “Tokai” which are partly grown under a yeast film.
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  • 68
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Immunoaffinity chromatography ; Proteins ; Surface plasmon resonance detection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The BIAcoreTM Biosensor System utilizes a detection principle known as Surface Plasmon Resonance (SPR) which, in simple terms, detects changes in the refractive index of a solution in contact with a gold film. The gold film is surface modified with carboxymethylated dextran to produce a hydrophillic matrix onto which macromolecules may be covalently immobilized. In this respect, the BIAcoreTM is similar to any other insoluble matrix, such as a chromatographic support. The SPR detection principle, however, allows one to directly ‘visualize’ the interaction under study, in real time and without the need for reporter molecules such as enzyme-labels. In addition, the very small sample requirements, automated robotics unit and ease of data analysis suggest the potential use of the BIAcoreTM instrumentation for assay development and possibly process development, especially where bio-specific interactions such as immunoaffinity chromatography are used as a step in the process. In this report, we demonstrate the use of the BIAcoreTM SPR detector for the micro-scale determination of conditions for immunoaffinity chromatography of soluble complementreceptor 1, sCR1.
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  • 69
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    Chromatographia 35 (1993), S. 5-12 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Post-column reaction detector ; Explosives
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Improvements in selectivity and sensitivity in the analysis of common explosives, like nitrate esters, nitramines and nitroaromatic compounds can be achieved by post-column derivatisation in a two step reaction detector. The first step in derivatisation is the photolysis of the analytes with UV at 254 nm. The photo reactor consists of a crocheted 20 m Tefzel capillary, which is coiled around a low pressure mercury lamp. In second step the nitrite ion generated is subsequently detected by a colourimetric reaction. The azo dye formed can be selectively detected at 540 nm. Addition of alkali after chromatographic separation to prevent oxidation of initially formed nitrite to nitrate during photolysis leads to a complex multistage arrangement. However, the contribution to peak broadening by the reactor is negligible and it is possible to detect 25–50 ppb of nitramines and 30–100 ppb of nitrate esters. Another advantage of the method is the selective detection of nitro compounds, even in complex matrices. The trace analysis of explosives is of growing interest in forensic science as well as in environmental analysis. It has been shown [1] that explosives can easily be extracted from soil and debris by the use of supercritical carbon dioxide. The separation and determination of explosives by gas chromatography is hindered by their thermal instability. In HPLC only the nitro aromatic explosives can be detected with sufficient sensitivity. Other types of explosives like the esters of nitric acid or nitramines do not absorb sufficiently in the UV region for sensitive detection. It has been shown [2] that explosives are liable to photochemical decomposition in the UV region, resulting in nitrate and nitrite, which have been detected after separation by ion-pair chromatography with electrochemical detection. A more sensitive and selective detection of nitrite has been possible in flow injection analysis [3]. Here a modified Griess reaction has been used. In a first step nitrite ions are used to form the diazonium salt with sulfanilamide which is coupled in a second step with N-[naphthyl-(1)]-ethylene diamine (NED) to form a redviolet azo dye with an absorption maximum at 540 nm. The advantage of this method is selective detection in the visible region, where hardly any other organic components are detected, which might be present in a crude environmental sample. In this paper the transfer of the Griess reaction to post-column derivatisation in RP chromatography of explosives will be described, and the optimisation of trace analysis of these solutes will be discussed.
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  • 70
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Reverse phase ; Optimization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary An interpretive optimization procedure in which pH can be one of the variables is presented with the emphasis on optimizing separations. When varying the pH in reversed-phase liquid chromatography the retention of ionogenic solutes will change. Thus, the selectivity between ionogenic and neutral solutes or between ionogenic solutes mutually can be optimized. However, pH also greatly affects the efficiency (plate count) and peak shape (asymmetry). Optimum selectivity (i.e. large differences in retention times) may be observed under conditions where peaks are broad and asymmetrical. Thus, it is essential to simultaneously consider retention, peak width and peak shape and their effects on separation (effective resolution) in pH-optimization studies. A procedure in which this is done is presented and applied to optimizing the separation of a synthetic mixture of selected pharmaceuticals. After initial experiments to establish the parameter space (boundaries for pH and binary methanol — water composition), twelve experiments are performed according to a 3×4 experimental design. At each loaction the retention, peak height, peak area and peak symmetry are recorded for each solute. These data are then used to build models for each of the four characteristics and for each solute. From this set of models the response surface, describing the quality of separation as a function of pH and composition, can be calculated. A variety of optimization criteria (quantifying quality of separation) can be used. The optimum corresponds to the highest point on the response surface.
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  • 71
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    Chromatographia 35 (1993), S. 38-44 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Fullerenes ; On-line and off-line identification
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The separation of fullerenes with a monomeric octadecylsilica bonded phase using n-hexane or toluene/methanol mobile phase systems is described. Analytical and preparative separations, incorporating on-line UV/VIS spectral measurements, confirmed the existence of large fullerenes such as C76, C78 and C84. However, isomers of C78 and C84 were not conclusively found.
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  • 72
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    Chromatographia 35 (1993), S. 50-54 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Wine ; Volatile phenols
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary An alternative to the traditional solvent extraction method used to extract and rapidly quantify ethyl-and vinylphenol and ethyl-and vinylgaiacol from wine is presented. The method is based on retention of volatile phenols on adsorbants. Among the tested resins, the most efficient, AG 2-X8 (anion exchange resin), worked as well with a synthetic solution as with wines. The percolation of clarified wine adjusted to pH 9 on this resin permits, in particular, the elimination of organic acids. Phenols are not eluted after rinsing the column with 1N HCl, but are eluted with methanol after this treatment. Good recovery (91 %) and good repeatability are observed. The eluate is directly analysed by HPLC on an RP18 column after two-fold dilution in water. The four volatile phenols were completely separated and detected by UV at 280 nm with high sensitivity (20–40 ppb). No interference with other compounds were noted in the different wines analysed.
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  • 73
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    Chromatographia 35 (1993), S. 61-66 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Sugars ; Ion-pair separation ; Pulsed electrochemical detection ; Hypercarb and PLRP-S
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Sugars, sugar acids, amino sugars and oligomers are separated as ion-pairs with hydrophobic counter ions at alkaline pH using PLRP-S and Hypercarb as solid phases. Important parameters for regulation of retention and selectivity are nature and concentration of the counter ion, pH (hydroxide concentration) and temperature. Reversals in the elution order wer obtained in some cases. Oligosaccharides are highly retained in these systems. The addition of organic modifiers to the mobile phase for elution of the solutes were found to interfere with the pulsed electrochemical detection (PED). Anions added to the mobile phase compete with the solutes for ion-pair retention, hence, decreasing the capacity factors, and phosphate could be used for this purpose in the separation of maltooligomers (M2-M10) from corn syrup.
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  • 74
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    Chromatographia 35 (1993), S. 55-60 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Carbohydrates ; Ion-pair separation ; Glycoconjugates ; PRP-1 and PLRP-S
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A new technique for the separation of carbohydrates as ion-pairs in strongly alkaline solution is presented. Carbohydrates are weakly acidic and partly present as anions at pH≥12 [1]. They are retained as ion-pairs on polymeric solid phases (PRP-1 and PLRP-S) with a hydrophobic quaternary ammonium counter ion present in the mobile phase. The effects of nature and concentration of mobile phase components on the retention of carbohydrates have been investigated and an ion-pair distribution model is proposed. The influence of temperature indicated no changes in retention mechanism with high counter ion concentration, but the resolution decreased with increasing temperature. Saccharides added to the mobile phase were shown to increase the retention and the selectivity.
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  • 75
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    Chromatographia 35 (1993), S. 90-92 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; α1-acid glycoprotein ; Complex glycans ; Allergic reaction ; Terfenadine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Little is known about the alterations that have occurred at the individual glycosylation sites in allergy patients or how these glycosylation patterns may change after anti-allergy treatments. Using reverse-phase HPLC, we have quantitated the glycoforms present at the individual glycosylation sites on orosomucoid isolated from the sera of allergic reaction patients and an allergic reaction patient treated with the antihistamine Terfenadine. The glycan structures isolated from the five glycosylation sites for the individual taking Terfenadine were all within normal ranges. It is suggested that if the changes in glycosylation in OMD in the allergic state are functionally driven, then it should be possible to correlate biological activities with quantitative changes at the individual glycosylation sites, and hence further define the role of OMD in allergy and inflammation.
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  • 76
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Bonded liquid crystals ; Polymeric stationary phases ; Variable temperature studies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Using two polycyclyic aromatic hydrocarbons as solutes, a comparison is made between a bonded liquid crystal stationary phase and a conventional polymeric C-18 phase. The bonded nematic liquid crystal phase was the silanized form of 4-[4-(allyloxy)benzoyl-oxy]biphenyl and the polymeric phase was Vydac 201TP. Both phases display shape and planarity selectivity as indicated by the results of the variable temperature and mobile phase composition studies. The slot theory of retention can be used to explain these results. However, the liquid crystal phase is more sensitive to molecular geometry, probably due to its more ordered structure on the surface. Variable temperature experiments which compare retention during both heating and cooling provides additional support for this conclusion. With the polymeric bonded C-18 phase, each solute had identical retention at the same temperature during both the heating and cooling cycles. On the bonded liquid crystal phase, measurable differences in retention were observed at identical temperatures depending on whether the column was heated or cooled. This effect is attributed to a degree of partially reversible disordering which occurs as the column temperature was increased. However, conditioning with the appropriate mobile phase can restore the original retention characteristics of the bonded liquid crystal phase.
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  • 77
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    Chromatographia 35 (1993), S. 133-138 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Ligand exchange chromatography ; Zirconium oxide ; Aluminum oxide ; Lewis acids and bases
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The chromatographic properties of alumina and zirconia are compared with respect to their relative Lewis acidities when parabenzoic acid derivatives are chromatographed in aqueous media. Weak Bronsted acids show similar capacity factors on alumina and zirconia. As solute Bronsted acidity increases, the capacity factor increases more significantly on the zirconia phase than on the alumina phase. Efficiencies, as determined by the dominant desorption rate constants, are generally comparable between the two phases with alumina showing slightly higher efficiencies, Secondary interactions between the solutes and the stationary phases are nearly absent on zirconia but are very apparent on the alumina phase.
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  • 78
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Formaldehyde in air ; Fluorimetric detection ; Comparison of fluorescent derivatives
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Three different fluorimetric methods for the measurement of atmospheric formaldehyde are described. The relatively unknown 2-diphenylacetylindan-1,3-dionhydrazine (DAIH) method was developed and optimized with an improved limit of detection of 2 ppbv corresponding to a sample volume of 7.5 L. Separation of lutidines necessary for the Nash method was successfully carried out and the separation time was reduced by a factor of 5. The detection limit of the Nash method was improved to 1 ppbv. In comparison, the N,N-diphenyl-1-naphthylamine-5-sulfonhydrazine (DNSH) method performed in a similar manner, reached an LOD of 0.5 ppbv. Finally, measurements made in candle smoke using the three different fluorimetric methods and three standard methods yielded very similar results.
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  • 79
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; 18 β-glycyrrhetinic acid ; Glycyrrhizinic acid ; Toothpastes ; Solid phase extraction (SPE)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary HPLC methods suitable for the selective and sensitive determination of 18 β-Glycyrrhetinic acid (GT) and Glycyrrhizinic acid (GZ) in toothpastes have been developed. The methods involve a preliminary quantitative solid phase extraction (C-18 sorbent) for sample clean-up and analyte concentration. Chromatographic separations were performed on reversed phase (C-8; C-18) columns using mixtures of methanol and phosphate buffers (pH 3.0) as the mobile phase under isocratic or gradient elution conditions. The method were applied successfully to the analysis of commercial toothpastes containing low levels (0.013–0.065%) of GT and GZ.
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  • 80
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Amphetamine in plasma ; Enantiomeric separation ; Solid phase derivatization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary An analytical approach has been developed for the one step determination of enantiomeric amphetamine composition in plasma, using on-line, pre-column solid phase derivatization with reversed phase HPLC separation. The high molecular weight protein components were excluded by the small pore structure of the polymer and washed out of the reaction column before derivatization. Spiked amphetamine in human plasma was extracted and derivatized by the polystyrene based FMOC-L-prolyl solid phase reagent. The derivatized diastereomers were separated on a conventional ODS column with an ACN/H2O mobile phase. No kinetic resolution or racemization was observed in this solid phase derivatization. Calibration plots and reproducibility experiments were performed to demonstrate the validity of the new approach. Automation of the procedure provided a simple and reproducible method for direct chiral recognition in plasma samples.
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  • 81
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Pre-column derivatisation ; Morpholine and degradation products ; Boiler feed water
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Morpholine and its amine-type thermal degradation products present in boiler feed water and steam condensate were derivatised with N-succinimidyl-p-nitrophenylacetate. These pre-column derivatives were determined by high-performance liquid chromatography with UV detection at 280 nm. The analytical column was Supelco-sil-ODS with an isocratic mobile phase. Morpholine and its breakdown products were monitored in the range 0.01–10 μg ml−1 with a relative standard deviation of 0.4–3.0%. Chromatographic analysis of boiler feed water and steam condensate samples collected from a boiler servicing a petroleum refinery is described.
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  • 82
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    Chromatographia 35 (1993), S. 189-192 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Carotenoid pigments ; Chlorophylls ; Photosynthestic tissue
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A high performance liquid chromatography system for the analysis of photosynthetic pigments is presented. The method employs an octadecylsilica stationary phase, a programmed quaternary mobile phase consisting of mixtures of methanol, acetonitrile, water and hexane, and a photodiode array detector. Carotenoids and chlorophylls are rapidly analysed in a single chromatographic separation. Thecis-trans isomers of most carotenoids are separated by this method.
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  • 83
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Monensins A and B ; Fermentation broth ; Streptomyces cinnamonensis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary High performance liquid chromatography on an octadecyl silica column has been used to determine both the monensin A: monensin B ratio and by the method of standard addition, the concentration of both in the fermentation broth ofStreptomyces cinnamonensis. Refractive index detection was preferred to ultraviolet owing to the presence of UV-absorbing components which could not be completely separated from the substances of interest. A linear relationship was obtained from the calibration data. The coefficients of variation for the estimation both of the ratio and the concentrations of the compounds were better then 5%. The estimated limit of detection for both substances was about 1 μg/ml. The results obtained from the determination of the ratios of monensins were compared with those obtained by chemical ionization mass spectrometry. Chromatographic separation of monensins on the silica gel column is also described.
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  • 84
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Ascorbic acid ; Antioxidant synergists ; Food, pharmaceuticals and cosmetics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A specific and sensitive reverse-phase HPLC method for the quantitative determination of ascorbic acid and antioxidant synergists (1-tartaric acid, citric acid, lactic acid as lithium lactate and EDTA) in fatty pharmaceuticals, cosmetics and food has been developed. Two extraction procedures were used; treatment with hot water, and extraction with water from a hexane dilution of the product. No significant differences between the two procedures were found (p〈0.05), except for ascorbic acid. Quantitative determinations were performed using a C-18 column and sulfuric acid (pH 1.95) mobile phase. With detection, at 210 nm, lactic acid overlapped with ascorbic acid, but the former could be readily identified by TLC. Ascorbic acid was detected at 254 nm, when lactic acid (as lithium lactate) did not interfere in the analysis. Mean recoveries for tartaric, citric and lactic acids were in the range 96–101%.
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  • 85
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Microdialysis ; Fermentation monitoring
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary An automated on-line sampling and analytical set-up for the control of fermentations was studied incorporating a microdialysis probe as the sampling device. Applications to a penicillin broth and an ethanol fermentation were studied. Typical recovery values of carbohydrates were found to be close to 100% even after exposure of the microdialysis probe in the process for about 30 h.
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  • 86
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    Chromatographia 35 (1993), S. 264-268 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Enantiomer separation ; Stereospecific synthesis ; Crown ether phase ; Immobilized enzyme reactor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A prototype of a coupled-column chromatographic system has been developed to determine the enantiomeric purity of enzymatically produced chiral compounds. In this system, an immobilized enzyme HPLC reactor based upon α-chymotrypsin (ACHT-IMER) was linked through a switching valve to a chromatographic column containing a crown ether-based chiral stationary phase (CR-CSP). Although ACHT is normally stereospecific for the L-enantiomorphs of aromatic amino acids, L-tryptophan (L-TRP), L-tyrosine and L-phenylalanine, we were able to achieve the hydrolysis of D-TRP methyl and ethyl esters on the ACHT-IMER. The percent of hydrolysis of the D-TRP esters could be manipulated by varying the resident time in the ACHT-IMER. In a series of experiments, D,L-TRP methyl or ethyl esters were injected into the ACHT-IMER, the flow stopped and after resuming the flow, the eluent which contained the hydrolyzed aminoacids, D- and L-TRP, was switched onto the CR-CSP where the enantiomeric composition of the TRP peak was determined. This configuration is a model for other IMER/CSP coupled-column systems which can be used for analytical and preparative applications.
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  • 87
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    Chromatographia 35 (1993), S. 275-280 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Thin-layer chromatography ; Anti-hypoxia drugs ; Lipophilic character and retention
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The lipophilicity of 14 anti-hypoxia drugs has been determined by reversed phase thin-layer (RPTLC) and reversed phase high performance liquid chromatography (RPHPLC) in eluent systems containing different concentrations of acetonitrile and potassium dihydrogen phosphate. There was significant correlation between lipophilicity and the specific hydrophobic surface area of the drugs in RPTLC, indicating that the drugs behave as an homologous series of compounds. In RPTLC the concentration of buffer has a negligible effect on the retention of the drugs whereas in RPHPLC the buffer concentration influenced the retention. This discrepancy can be explained by the lower sensitivity of RPTLC. There was strong correlation between lipophilicity values determined by both methods, proving that both are suitable for the determination of molecular lipophilicity.
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  • 88
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; 1,4-Benzoxazin-3-ones ; Cyclic hydroxamic acids ; β-Cyclodextrin modified silica gel ; Enantiomer separation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A liquid chromatographic procedure on a 5 μm LiChroCART column using chemically bonded β-cyclodextrin (ChiraDex) as chiral selector was developed for the enantiomeric separation of four 2H-1,4-benzoxazin-3(4H)-ones which are close analogs of the cyclic hydroxamic acids 2,4-dihydroxy-2H-1,4-benzoxazin-3(4H)-one (DIBOA) and 2,4-dihydroxy-7-methoxy-2H-1,4-benzoxazin-3 (4H)-one (DIMBOA) naturally occurring inGramineae species.
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  • 89
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    Chromatographia 35 (1993), S. 295-301 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Used column packings ; Particle size distribution ; Scanning electron microscopy ; Column age
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Particle size distribution analysis and scanning electron microscopy (SEM) were carried out on eight used HPLC columns containing either irregular silica based, spherical silica based or spherical polymer based packing material. Particle size distributions of the used irregular silica based columns were at least bimodat at the outlet ends and either biomodal or log-normal at the inlet ends with regular progressions between the two extremes through the column. A new ODS-3 column showed log-normal size distributions from the inlet to the outlet ends. Spherical silica based column particle size distributions showed distinct shoulders on large central distribution peaks in most column sections with various degrees of shoulder erosion. The spherical resin based column showed a broader inlet particle size distribution progressing to a very narrow outlet distribution. SEMs of both irregular and spherical silica based columns revealed a larger number of undersized particles and debris at the outlet than inlet ends which could have resulted from stationary phase degradation, since this was not seen in the new ODS-3 column. While several SEMs of the spherical silica based columns revealed hollow spheres and twins, the spherical resin based column packing showed stress fractures or wrinkle lines resulting from use or dehydration.
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  • 90
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    Chromatographia 35 (1993), S. 311-316 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Isolation of natural products ; Solid phase extraction ; Chemically bonded phase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Esculin (ESC) and rutin (RUT) have been simultaneously isolated from pharmaceutical natural materials by solid phase extraction (SPE). Determination of both substances was performed by reversed phase high performance liquid chromatography (RPHPLC) with UV detection. Optimization of the separation conditions showed that simultaneous isolation and determination of rutin and esculin from pharmaceutical material was possible. The recovery obtained was not lower than 95±2%.
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  • 91
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Enantiomer separation ; Chiral ovomucoid column ; Diltiazem hydrochloride ; Clentiazem maleate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A direct HPLC separation method was developed for the determination of the enantiomers of racemic precursors to diltiazem (I) and its 8-chloro derivatives (II). The enantiomers were successfully separated on a chiral ovomucoid column using an aqueous-organic mobile phase (reversed-phase HPLC). The influence of the organic modifier and buffer pH on the retention and enantioselectivity was investigated. The chromatographic conditions chosen for the separation permitted complete resolution of the enantiomers of both the acid (Ib and IIb) and methyl ester precursors (Ia and IIa) within 20 min. The influence of sample load on retention times, theoretical plates numbers, peak heights and peak areas was also investigated. The peak areas showed a good linearity over the concentration range examined, although all the others were influenced significantly by the sample size. An optical antipode of the intermediate to be determined could be detected by the area-percentage method down to ca. 0.1%, together with the determination of its precursor, including its optical purity.
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  • 92
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Probenecid in urine ; Solid-phase extraction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary This study describes a rapid method for the determination of probenecid in human urine by liquid chromatography with UV detection at 254 nm, after clean-up through a C8 solid-phase extraction column. Liquid chromatography was carried out on a C18-bonded phase using an acetonitrile-acetate buffer (pH=4) gradient elution. Ethacrynic acid was used as internal standard. The system has been applied to the determination of probenecid in the 0.10–100.0 μg/ml concentration range; the limit of detection was 5 ng/mL.
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  • 93
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    Chromatographia 35 (1993), S. 390-394 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Electrostatic model ; Ion-pair reagent ; Organic modifier ; Prediction of k′ values
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The capacity factors of phenylamine and naphthylamine sulphonic acids in reversed-phase, ion-pair liquid chromatography (RP-IPC) were measured. The combined effects of organic modifier (Cb) and ion-pair reagent concentration (Cp) on retention follow an equation based on the electrostatic model: $$\ln k' = a + b lnC_p + cC_b $$ the experimental value of b correlates well with the value of 0.5 z (z=charge on analyte ion) predicted for mono and divalent ions. The measured value of b is, however, lower than that predicted for trivalent ions, which may be due to the effective number of charges being less than the apparent number. The absolute values of a and c are much larger than those in RP-HPLC in the absence of an ion-pair reagent, quantitative correlation of a and c with retention values in RP-HPLC and solute charges has been observed and a good linear relationship between a and c has been obtained, strongly supporting the validity of the electrostatic retention model. A critical value, R, at which the negative effect of methanol on retention is equal to the positive effect of the ion-pair reagent (TBAI) has been proposed. The critical values obtained are related to the behaviour of the solute, the ion-pair reagent and the stationary and mobile phases, and lies between −0.04 and −0.065 in most cases, which means that the increase by exp times (=2.718×) the ion-pair concentration the original is equivalent to a decrease of 0.04–0.065 in volume fraction of methanol
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  • 94
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    Chromatographia 35 (1993), S. 381-386 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Bovine trypsin ; Porcine kallikrein ; Bovine pancreatic trypsin inhibitor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The possibility of determining more than one enzyme at the same time has been examined. A new approach, based on the measurement of a direct and specific chromatographic signal obtained by hydrophobic interaction chromatography (HIC) of the stable complex formed with the inhibitor aprotinin, is proposed. A basic procedure for the quantitative determination of trypsin and kallikrein, taken as models, is described. The method is precise with a mean coefficient of variation of 3.1% and 3.5% for trypsin and kallikrein, respectively; the limit of determination for both enzymes is 0.17 nmol ml−1 in the original sample.
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  • 95
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    Chromatographia 35 (1993), S. 387-389 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Buffer composition effect ; Agarose ; Albumin ; Ionic adsorption
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The adsorption of albumin on a commercial, high quality agarose (Sepharose® 6B) was studied in different buffers of low pH (4.00) and low molarity (9–11 mM). Eluents containing 0.025% albumin were introduced onto small gel columns until saturation and the adsorption capacity of the gel in different buffers calculated. Adsorption was high in solutions of monobasic acids. Although cation-exchange interactions between the protein and the gel matrix were found to be influenced essentially by the sodium ion concentration or ionic strength of the buffer, adsorption in cirate buffers is weak compared to that in formate, acetate or propionate buffers of about the same ionic strength (I=0.01).
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  • 96
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Polyunsaturated fatty acids ; Direct injection ; Enthalpy of adsorption
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Retention studies on arachidonic acid 5, 8, 11, 14 all cis eicosatetraenoic acid, C20:4ω6), eicosapentaenoic acid (5, 8, 11, 14, 17 all cis eicosapentaenoic acid, C20∶5ω3) and docosahexaenoic acid (4, 7, 10, 13, 16, 19 all cis docosahexaenoic acid C22∶6ω3) were performed on a commercial μBondapak free fatty acid analysis column. The ternary mobile phase consisting of acetonitrile, water and tetrahydrofuran was used in an isocratic mode with differential refractometry detection. Retention data were mesured at various flow rates with two different, mobile-phase compositions. Capacity factors and enthalpy of adsorption were calculated from the retention data. Finally the retention mechanism is explained.
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  • 97
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    Chromatographia 35 (1993), S. 403-409 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Reversed phase packings ; Polybutadiene coating ; Polymer coatings
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Non-wetting viscous liquids such as oligobutadiene prefer “active” sites such as pores during the process of physisorption. Thus, polybutadiene (PBD) coatings on porous silica do not result in a homogeneous polymer film but in an inhomogeneous loading where the bulk polymer is mainly sited in the pores of the silica. An increasing polymer loading leads to increasingly filled pores instead of a thicker polymer film. We cannot exclude the possibility that most of the surface is covered at least with a thin polymer film since the chromatographic behaviour is relatively good for polypeptides, which are highly susceptible to the silanol groups of silica.
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  • 98
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    Chromatographia 35 (1993), S. 435-438 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Pirarubicin and metabolites ; Anthracyclines
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary A new and highly sensitive HPLC method for the simultaneous determination of pirarubicine (THP-doxorubicin) and its metabolites, adriamycin and adriamycinol, in human plasma, is described. Samples were treated by liquid-liquid extraction, the organic phase removed and the residue dissolved in methanol. Separation was on a Lichrocart Supersher RP 8 column, (250×4 mm) 4 μm, with a mobile phase of acetonitrile/methanol/formate-buffer.
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  • 99
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    Chromatographia 35 (1993), S. 439-447 
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; Affinity chromatography ; Polythiolpeptides and metallopolythiolpeptides ; Mechanism of separation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary Covalent affinity chromatography with thiol-disulphide interchange (CAD-TDI) is a method for the separation of thiolproteins based on the interaction between disulphide bridges immobilised on a insoluble support and thiol groups in the protein undergoing separation. Mercury-thionein, cadmium-thionein and apothionein have been used as low molcular weight thiolproteins rich in cysteine residue. The proposed separation mechanism is more complex than those in the literature and can have anionic, cationic or a free radical character depending on the protein, the ligand and the conditions during separation.
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  • 100
    ISSN: 1612-1112
    Keywords: Column liquid chromatography ; UV and electrochemical detectors ; Phenolic antioxidants ; Migration studies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Summary The performance of UV diode-array, spectrometric and electrochemical detectors was compared in the chromatographic analysis of trace amounts of six phenolic antioxidants. Quantitative validation was undertaken; the linearity range was wider using UV detection although the limits of detection were lower with electrochemical detection. UV detection was applied both to identification of an antioxidant in a hydrophilic suspension and to a migration study.
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