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  • Zea mays  (43)
  • stability  (40)
  • Springer  (82)
  • American Meteorological Society
  • National Academy of Sciences
  • Periodicals Archive Online (PAO)
  • 1990-1994  (82)
  • 1935-1939
  • 1992  (82)
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  • Springer  (82)
  • American Meteorological Society
  • National Academy of Sciences
  • Periodicals Archive Online (PAO)
  • Wiley-Blackwell  (5)
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  • 1990-1994  (82)
  • 1935-1939
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  • 1
    Electronic Resource
    Electronic Resource
    Characterization of stable matchings as extreme points of a polytope (1992)
    Springer
    Mathematical programming 54 (1992), S. 57-67 
    Details
    ISSN: 1436-4646
    Keywords: Matchings ; stability ; extreme points ; polytope
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science , Mathematics
    Notes: Abstract The purpose of this paper is to extend a modified version of a recent result of Vande Vate (1989) which characterizes stable matchings as the extreme points of a certain polytope. Our proofs are simpler and more transparent than those of Vande Vate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01586041
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  • 2
    Electronic Resource
    Electronic Resource
    Phthalocyanine-containing polymers (1992)
    Springer
    Journal of inorganic and organometallic polymers and materials 2 (1992), S. 79-85 
    Details
    ISSN: 1572-8870
    Keywords: Phthalocyanines ; polymers ; siloxanes ; conductivity ; stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The author's work on the incorporation of phthalocyanines into inorganic polymers is reviewed. The synthesis of poly(siloxane phthalocyanines) and the synthesis and characterization of fluoro(phthalocyanine) Group III compounds and their conducting derivatives are described.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00696537
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  • 3
    Electronic Resource
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    Analytical relationships between the ionization of 1,3-dicarbonylic ligands and stability of their Cr(III), Ni(II), Co(II) and Cu(II) 1∶1 chelates (1992)
    Springer
    Journal of solution chemistry 21 (1992), S. 1145-1152 
    Details
    ISSN: 1572-8927
    Keywords: Chromium(III) ; β-diketones ; β-diketonates ; chelation ; complexation ; correlation ; stability ; equilibrium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract It has been demonstrated that the logarithm of the stability constant of some monochelated chromium(III) compounds, with structurally similar 1,3-dicarbonylic species, is linearly related to the negative logarithm of the acid ionization constant of the ligand. Graphical and analytical correlations which could be useful in predicting equilibrium constants of chromium(III)-β-diketonates, as well as other first-row transition metal derivatives, have been developed. A quantitative evaluation of the complexes stability has been carried out, providing information about the effects of ligand substituents on the equilibrium constants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00651860
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  • 4
    Electronic Resource
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    Interval stability and interval covering property in finite posets (1992)
    Springer
    Order 9 (1992), S. 163-175 
    Details
    ISSN: 1572-9273
    Keywords: Primary 06A07 ; secondary 05C70 ; Partial order ; interval ; stability ; covering ; Sperner property ; symmetric chains ; NP-completeness
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract Given a finite ranked posetP, let α(P) be the maximum size of a subset ofP such that no two elements of it belong simultaneously to some interval ofP and let ϱ(P) be the minimum number of intervals covering all elements ofP. We say thatP has the strong interval stability property (resp. the strong interval covering property) if for each subposetP′ induced by consecutive levels ofP, i.e.,P′=P (l)∪...∪P (u), one has α(P′)=max{|P (l)|, |P (u)|} (resp. ϱ(P′)=max{|P (l)|, |P (u)|}). We prove these properties for several classes of posets and discuss some general facts concerning the numbers α(P) and ϱ(P), e.g., NP-completeness and min-max relations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00814408
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  • 5
    Electronic Resource
    Electronic Resource
    Stationary waiting time derivatives (1992)
    Springer
    Queueing systems 12 (1992), S. 369-389 
    Details
    ISSN: 1572-9443
    Keywords: Perturbation analysis ; stability ; stochastic difference equations ; simulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Notes: Abstract We investigate the stability of waiting-time derivatives when inputs to a queueing system-service times and interarrival times-depend on a parameter. We give conditions under which the sequence of waiting-time derivatives admits a stationary distribution, and under which the derivatives converge to the stationary regime from all initial conditions. Further hypotheses ensure that the expectation of a stationary waiting-time derivative is, in fact, the derivative of the expected stationary waiting time. This validates the use of simulation-based infinitesimal perturbation analysis estimates with a variety of queueing processes. We examine waiting-time sequences satisfying recursive equations. Our basic assumption is that the input and its derivatives are stationary and ergodic. Under monotonicity conditions, the method of Loynes establishes the convergence of the derivatives. Even without such conditions, the derivatives obey a linear difference equation with random coefficients, and we exploit this fact to find stability conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01158809
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  • 6
    Electronic Resource
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    Stability of token passing rings (1992)
    Springer
    Queueing systems 11 (1992), S. 7-33 
    Details
    ISSN: 1572-9443
    Keywords: Token passing rings ; stability ; substability ; ergodicity ; Markov chains ; Loynes' scheme ; stochastically dominant ; Little's formula ; regenerative processes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Notes: Abstract A sufficient stability condition for the standard token passing ring has been “known” since the seminal paper by Kuehn in 1979. However, this condition was derived without formal proof, and the proof seems to be of considerable interest to the research community. In fact, Watson observed that in the performance evaluation of token passing rings, “it is convenient to derive stability conditions ... (without proof)”. Our intention is to fill this gap, and to provide a formal proof of thesufficient and necessary stability condition for the token passing ring. In this paper, we present the case when the arrival process to each queue is Poisson but service times and switchover times are generally distributed. We consider in depth a gatedl-limited (l≤ ∞) service discipline for each station. We also indicate that the basic steps of our technique can be used to study the stability of some other multiqueue systems.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01159285
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  • 7
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    Stable steady state of some population models (1992)
    Springer
    Journal of dynamics and differential equations 4 (1992), S. 161-190 
    Details
    ISSN: 1572-9222
    Keywords: Delay differential equations ; equilibrium ; stability ; limiting equations ; population dynamics ; 34K20 ; 34K25 ; 92A15
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract Applying an analytical method and several limiting equations arguments, some sufficient conditions are provided for the existence of a unique positive equilibriumK for the delay differential equationx=−γx+D(x t ), which is the general form of many population models. The results are concerned with the global attractivity, uniform stability, and uniform asymptotic stability ofK. Application of the results to some known population models, which shows the effectiveness of the methods applied here, is also presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01048159
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  • 8
    Electronic Resource
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    Stability estimates in inverse scattering (1992)
    Springer
    Acta applicandae mathematicae 28 (1992), S. 1-42 
    Details
    ISSN: 1572-9036
    Keywords: 35R30 ; Inverse scattering ; stability ; noisy data
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract An algorithm is given for calculating the solution to the 3D inverse scattering problem with noisy discrete fixed energy data. The error estimates for the calculated solution are derived. The methods developed are of a general nature and can be used in many applications: in nondestructive evaluation and remote sensing, in geophysical exploration, medical diagnostics, and technology.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00047029
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  • 9
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    A new interpolation procedure for adapting Runge-Kutta methods to delay differential equations (1992)
    Springer
    BIT 32 (1992), S. 634-649 
    Details
    ISSN: 1572-9125
    Keywords: AMS(MOS): 65L20 ; CR: 5.17 ; delay differential equations ; numerical solution ; Runge-Kutta methods ; interpolation procedures ; stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract This paper deals with adapting Runge-Kutta methods to differential equations with a lagging argument. A new interpolation procedure is introduced which leads to numerical processes that satisfy an important asymptotic stability condition related to the class of testproblemsU′(t)=λU(t)+μU(t−τ) with λ, μ ε C, Re(λ)〈−|μ|, and τ〉0. Ifc i denotes theith abscissa of a given Runge-Kutta method, then in thenth stept n−1→t n :=t n−1+h of the numerical process our interpolation procedure computes an approximation toU(t n−1+c i h−τ) from approximations that have already been generated by the process at pointst j−1+c i h(j=1,2,3,...). For two of these new processes and a standard process we shall consider the convergence behaviour in an actual application to a given, stiff problem.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01994847
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  • 10
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    On a class of variational equations transformable to the Gauss hypergeometric equation (1992)
    Springer
    Celestial mechanics and dynamical astronomy 53 (1992), S. 145-150 
    Details
    ISSN: 1572-9478
    Keywords: Monodromy matrix ; Gauss hypergeometric equation ; stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract A new class of linear ordinary differential equations with periodic coefficients is found which can be transformed to the Gauss hypergeometric equation, and therefore the monodromy matrices are computable explicitly. These equations appear as the variational equations around a straight-line solution in Hamiltonian systems of the form H = T(p) + V(q), where T(p) and V(q) are homogeneous functions of p and q, respectively.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00049462
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  • 11
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    Effects of small external forces on the planar oscillation of a cable connected satellites system (1992)
    Springer
    Celestial mechanics and dynamical astronomy 53 (1992), S. 219-226 
    Details
    ISSN: 1572-9478
    Keywords: Artificial satellite ; dissipative forces ; stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The effects of small external dissipative and disturbing forces on the non-linear planar oscillation of a cable connected satellites system in the central gravitational field of earth have been studied. Typical non-linear oscillation's phenomena arizing from the aforesaid external forces are shown to take place. The presence of these forces enables the application of asymptotic methods of the theory of non-linear oscillations due to Bogoliubov and Mitropolsky to the equation characterizing the non-linear oscillation of the system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00052610
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  • 12
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    Real-time feedback scheduling of flexible manufacturing systems (1992)
    Springer
    Journal of intelligent and robotic systems 6 (1992), S. 51-63 
    Details
    ISSN: 1573-0409
    Keywords: Scheduling ; flexible manufacturing systems ; stability ; feedback
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract A genetic manufacturing environment is considered. The emphasis is on small-lot, discrete, and asynchronous type of manufacturing systems rather than high volume and continuous type. Two classes of scheduling policies are proposed to render the machine stable. The policies are of feedback type. The decision is made in real-time and on-line.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00314697
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  • 13
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    Force control of robots with nonlinearities (1992)
    Springer
    Journal of intelligent and robotic systems 6 (1992), S. 219-240 
    Details
    ISSN: 1573-0409
    Keywords: Force control ; slip-stick friction ; stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract This paper explores two practical issues related to the force control of manipulators. The first issue examined is how system stability is effected by commonly occurring manipulator nonlinearities, such as sampled-data, control signal saturation and slip-stick friction. It is shown that discretely implemented force control algorithms can drive the feedback force controlled manipulator into a limit cycle, even for a very small sampling period that by far satisfies Shannon's sampling theorem. The bounds of stability are enhanced by the presence of control signal saturation and slip stick friction. The second issue investigated is the inclusion of a high gain inner position loop as a means to minimize the unpredictability in the steady state error due to slip-stick friction. In order to support the theoretical conclusions, experiments were performed with the PUMP 560 industrial robot testbed facility developed at Colorado State University.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00248017
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  • 14
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    A structural approach to robust stability of polynomials (1992)
    Springer
    Multidimensional systems and signal processing 3 (1992), S. 421-425 
    Details
    ISSN: 1573-0824
    Keywords: Convex combination ; polynomial ; stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Electrical Engineering, Measurement and Control Technology
    Notes: Abstract A sufficient condition for Hurwitz stability of a convex combination of two stable polynomials is obtained.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01940234
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  • 15
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    Sequential development of pathogens in the maize tarspot disease complex (1992)
    Springer
    Mycopathologia 117 (1992), S. 157-161 
    Details
    ISSN: 1573-0832
    Keywords: Phyllachora maydis ; Monographella maydis ; Coniothyrium phyllachorae ; Zea mays ; tarspot complex
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The tarspot complex is caused by the interaction of Phyllachora maydis and Monographella maydis. Coniothyrium phyllachorae, possibly a mycoparasite, is found in older ascostromata of P. maydis, which always appears first causing tarspot. M. maydis follows and is responsible for the damaging “fisheye” symptom. The fisheye symptom is always associated with a tarspot in the center of the lesion, whereas 12 to 20% of the Phyllachora ascostromata remained free of M. maydis. Inoculations of maize leaves with the Microdochium anamorph of the Monographella (usually produced in lesions) failed to produce infections. Some infections with M. maydis were, however, obtained under unusual conditions in the field. Inoculations onto tarspots in the laboratory were unsuccessful, but in field experiments inoculations with conidia of M. maydis enhanced severity of the tarspot complex. Fisheye symptoms of the complex naturally appear 2 to 7 days after the manifestation of P. maydis. This is followed a week later by the appearance of M. maydis which became predominant in the lesions and is associated with empty perithecia of P. maydis. In the early stages of the tarspots pycnidia of the anamorph of P. maydis, Linochora sp., could occasionally be observed. Ascomata of M. maydis were rare in the field. Of the 36 genetic materials of CIMMYT tested, 30 developed the fisheye symptom, 4 tarspots only and 2 remained free of symptoms
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00442777
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  • 16
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    The effects of aflatoxin B1 on immature germinating maize (Zea mays) embryos (1992)
    Springer
    Mycopathologia 119 (1992), S. 181-190 
    Details
    ISSN: 1573-0832
    Keywords: aflatoxin B1 ; electron microscopy ; in vitro ; immature maize embryo ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Immature maize (Zea mays L.) embryos were treated with aflatoxin B1 concentrations, ranging from 0.1 μg ml−1 to 25 μg ml−1. Below 5 μg ml−1 aflatoxin B1, root and shoot elongation was not significantly inhibited. Ultrastructurally, root tip cells showed little deterioration, except a possible diffused clearing in mitochondria and plastids. As the toxin concentration was increased above 5 μgml−1, shoot, and particularly root elongation, was progressively inhibited. Associated with this, there was an apparent decrease in the ribosome population. Furthermore, membranes, particularly the vacuolar membrane, became abnormal and vacuolar distension occurred. At 20 and 25 μg ml−1, these effects were exacerbated, and mitochondria and plastid structure was disrupted. At these concentrations, there was evidence of a disruption in lipid metabolism. The results are discussed in the context of known aflatoxin effects on cellular control mechanisms and ultrastructure in animal systems.
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    URL: http://dx.doi.org/10.1007/BF00448817
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  • 17
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    Fertilizer value of pig slurries processed by the Promest procedure (1992)
    Springer
    Nutrient cycling in agroecosystems 32 (1992), S. 61-70 
    Details
    ISSN: 1573-0867
    Keywords: Pig slurry ; processing ; relative efficiency index ; N ; P ; K ; organic carbon ; stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In the Netherlands, pig slurries are processed to improve their fertilizer value as well as handling and application. The relative efficiency index (REI) of N, P and K and the stability of organic carbon (C) in test products of processed pig slurries (PPS) were determined in pot and incubation experiments. The dry and easily manageable PPS were produced according to the Promest procedure. The mineral fraction of total N varied from 60 to 88%. It consisted mainly of nitrate. About 25% of the organic N was rapidly mineralized. The REI of N varied from 66 to 92%. After application REI of P from pelletized PPS varied from 41 to 62% in the first half year. The REI of P in ground but non-pelletized PPS varied from 81 to 100%. The REI of K was estimated to be 100%. PPS contained less easily decomposable organic C than untreated pig slurry due to the anaerobic digestion step in the processing procedure. About 34% of the organic C disappeared as methane and carbon dioxide during processing. It was concluded that processing improved the fertilizer value of PPS compared with that of untreated pig slurry.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01054395
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  • 18
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    A reinforcement connectionist approach to robot path finding in non-maze-like environments (1992)
    Springer
    Machine learning 8 (1992), S. 363-395 
    Details
    ISSN: 0885-6125
    Keywords: Connectionism ; reinforcement learning ; robot path finding ; stability ; reactive systems
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Notes: Abstract This paper presents a reinforcement connectionist system which finds and learns the suitable situation-action rules so as to generate feasible paths for a point robot in a 2D environment with circular obstacles. The basic reinforcement algorithm is extended with a strategy for discovering stable solution paths. Equipped with this strategy and a powerful codification scheme, the path-finder (i) learns quickly, (ii) deals with continuous-valued inputs and outputs, (iii) exhibits good noise-tolerance and generalization capabilities, (iv) copes with dynamic environments, and (v) solves an instance of the path finding problem with strong performance demands.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00992702
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  • 19
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    A Reinforcement Connectionist Approach to Robot Path Finding in Non-Maze-Like Environments (1992)
    Springer
    Machine learning 8 (1992), S. 363-395 
    Details
    ISSN: 0885-6125
    Keywords: Connectionism ; reinforcement learning ; robot path finding ; stability ; reactive systems
    Source: Springer Online Journal Archives 1860-2000
    Topics: Computer Science
    Notes: Abstract This paper presents a reinforcement connectionist system which finds and learns the suitable situation-action rules so as to generate feasible paths for a point robot in a 2D environment with circular obstacles. The basic reinforcement algorithm is extended with a strategy for discovering stable solution paths. Equipped with this strategy and a powerful codification scheme, the path-finder (i) learns quickly, (ii) deals with continuous-valued inputs and outputs, (iii) exhibits good noise-tolerance and generalization capabilities, (iv) copes with dynamic environments, and (v) solves an instance of the path finding problem with strong performance demands.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1022684924132
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  • 20
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    Electroporation and PEG delivery of DNA into maize microspores (1992)
    Springer
    Plant cell reports 11 (1992), S. 567-570 
    Details
    ISSN: 1432-203X
    Keywords: Microspore ; Electroporation ; Transformation ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The ability to deliver and detect reporter gene activity in maize microspores was tested. Tested expression vectors contained the chloramphenicol acetyl transferase (CAT) gene and one of the following promoter-intron combinations: 1) cauliflower mosaic virus (CaMV 35S), 2) CaMV 35S + maize alcohol dehydrogenase 1 intron 6 (Adh1-I6), 3) maize alcohol dehydrogenase 1 + intron 1 (Adh1-I1), or 4) maize ubiquitin 1 + intron 1 (Ubiq 1-I1) promoter + intron. The expression vectors were delivered into maize microspores using electroporation or polyethylene glycol (PEG). Both methods were effective for delivering free DNA into microspores. Although all four promoters were active in maize protoplasts, only two promoters were active in maize microspores. The CaMV 35S and the Adh1 promoters did not promote gene expression in maize microspore. The CaMV 35S + Adh1-I6 and Ubiq1-I1 promoters produced high levels of CAT activity in maize microspores.
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    URL: http://dx.doi.org/10.1007/BF00233094
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  • 21
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    Cleavage polyembryony in maize (1992)
    Springer
    Sexual plant reproduction 5 (1992), S. 224-226 
    Details
    ISSN: 1432-2145
    Keywords: Zea mays ; Maize ; Polyembryony
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two types of cleavage polyembryony are described in the inbred line VIR 17 of maize. Suspensorial embryony was observed to occur spontaneously. Typical cleavage of the zygotic proembryo occurred spontaneously, but could also be induced by treating the developing caryopses with 2,4-Dichlorophenoxyacetic acid (2,4-D) on the second day after pollination. 2,4-D was active as a decorelative factor also evoking the expression of totipotency in individual proembryonal cells.
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    URL: http://dx.doi.org/10.1007/BF00189815
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  • 22
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    Analysis of pollen-tube growth in cultured maize silks (1992)
    Springer
    Sexual plant reproduction 5 (1992), S. 227-231 
    Details
    ISSN: 1432-2145
    Keywords: Zea mays ; Maize ; Pollen-tube growth regulation ; In vitro pollination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary In vitro pollen-tube growth in maize was studied using an in vitro pollination system. In the ‘cut-silk’ method, ovaries with silks were placed on medium in vitro, whereafter the silk was cut and the upper part of the silk was pollinated. Pollen tubes were not able to bridge the space between the two silk parts. Even when silk parts were tightly connected, pollen tubes still were not able to pass the cut ends and reach the lower silk part. Pollen-tube growth rates and the direction of tube growth were not influenced by the presence or absence of an ovary. Prepollination did not have any influence on pollen-tube growth rate. Measurements of pollen-tube growth rate also showed that there was no ‘population effect’, i.e. growth rate was not stimulated by pollination with an excess of pollen grains. We found that the direction in which maize pollen grew was determined only by the positioning of the silk hairs.
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    URL: http://dx.doi.org/10.1007/BF00189816
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  • 23
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    Maturation of maize pollen in vitro (1992)
    Springer
    Plant cell reports 11 (1992), S. 535-539 
    Details
    ISSN: 1432-203X
    Keywords: Zea mays ; in vitro culture ; in vitro pollen ; pollen germination ; fertilization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Maturation of maize pollen was obtained in male reproductive structures cultured in vitro. Immature tassels containing microspores at the mid-uninucleate to late-binucleate stage of development were excised and spikelets, anthers, and/or isolated microspores were cultured on a medium capable of supporting pollen maturation. Microspore mitosis, culminating in the production of starch-filled, trinucleate pollen capable of germination, was observed after 7–15 days, depending on the genotype and stage at which the cultures were initiated. Up to 100%, 70%, and 20% of the cultured spikelets, anthers, and isolated microspores, respectively, produced mature pollen, which germinated, however, at different frequencies (i.e., spikelets, 50–70%; anthers, 5–10%; microspores, 〈1%). Mature kernels were produced following fertilization with pollen from cultured spikelets and anthers. These procedures provide methods for the in vitro manipulation of a significant phase of the maize life cycle.
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    URL: http://dx.doi.org/10.1007/BF00236273
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  • 24
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    Matrix-associated DNA from maize is enriched in repetitive sequences (1992)
    Springer
    Plant cell reports 11 (1992), S. 355-358 
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    ISSN: 1432-203X
    Keywords: Zea mays ; Matrix-associated ; DNA ; repetitive sequences ; DNA loops
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In order to elucidate some features of the topological organization of DNA within the plant nucleus, DNA fragments involved in the attachment of the DNA loops to the nuclear matrix in maize were studied. The matrix-associated DNA from dry embryo and meristematic cells after extensive digestion with DNase I and high salt treatment was about 2% of the total DNA, sized within the range of 50 and 250 bp. This DNA was found to be enriched in repetitive DNA sequences, both for nuclei from dry embryo and meristematic cells. The loop size of the DNA in cells of Zea mays appeared to be between 5 and 25 kbp.
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    Field evaluation of N2 fixation and N partitioning in climbing bean (Phaseolus vulgaris L.) using 15N (1992)
    Springer
    Biology and fertility of soils 13 (1992), S. 142-146 
    Details
    ISSN: 1432-0789
    Keywords: A value ; Common bean ; N remobilization ; Soil N balance ; Atom% 15N excess ; Phaseolus vulgaris ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The common bean (Phaseolus vulgaris L.) is generally regarded as a poor N2 fixer. This study assessed the sources of N (fertilizer, soil, and fixed N), N partitioning and mobilization, and soil N balance under field conditions in an indeterminate-type climbing bean (P. vulgaris L. cv. Cipro) at the vegetative, early pod-filling, and physiological maturity stages, using the A-value approach. This involved the application of 10 and 100 kg N ha-1 of 15N-labelled ammonium sulphate to the climbing bean and a reference crop, maize (Zea mays L.). At the late pod-filling stage (75 days after planting) the climbing bean had accumulated 119 kg N ha-1, 84% being derived from fixation, 16% from soil, and only 0.2% from the 15N fertilizer. N2 fixation was generally high at all stages of plant growth, but the maximum fixation (74% of the total N2 fixed) occurred during the interval between early (55 days after planting) and late podfilling. The N2 fixed between 55 and 75 days after planting bas a major source (88%) of the N demand of the developing pod, and only about 11% was contributed from the soil. There was essentially no mobilization of N from the shoots or roots for pod development. The cultivation of common bean cultivars that maintain a high N2-fixing capacity especially during pod filling, satisfying almost all the N needs of the developing pod and thus requiring little or no mobilization of N from the shoots for pod development, may lead to a net positive soil N balance.
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    URL: http://dx.doi.org/10.1007/BF00336269
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    Odors influence choice of oviposition sites byDiabrotica virgifera virgifera (Coleoptera: Chrysomelidae) (1992)
    Springer
    Journal of chemical ecology 18 (1992), S. 1227-1237 
    Details
    ISSN: 1573-1561
    Keywords: Western corn rootworm ; Diabrotica virgifera virgifera ; Coleoptera ; Chrysomelidae ; bacteria ; carbon dioxide ; pheromone ; semiochemicals ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract FemaleDiabrotica virgifera virgifera LeConte were allowed to choose between oviposition substrates that were and those that were not associated with potential sources of semiochemicals. Females deposited over five times more eggs on moist towelettes that were treated with homogenates of female abdomens than on towelettes treated with distilled water. Similar results were obtained when screening separated the homogenates from the towelettes, indicating that odors alone could elicit the response. In contrast, females did not choose towelettes that had previously been used for oviposition or towelettes containing eggs over unused towelettes. Further tests with homogenates of abdomens and a bacteriostatic agent (sorbate) indicated that the females were probably responding to bacterial odors rather than an oviposition-enhancing pheromone. Four strains of bacteria were isolated from a homogenate of female abdomens; females deposited 4 to 16 times more eggs on substrates with odors of the bacteria than on substrates with odors of uninoculated nutrient agar. In no-choice tests, bacterial odors did not increase the number of eggs deposited per female beetle; however, in choice tests with dishes that tended to retain any beetles that entered, there were more eggs per female (but not more beetles) after 24 hr in dishes with bacterial odors than in those without the odors. Females also chose dishes with odors of excised maize (Zea mays L.) roots or elevated levels of carbon dioxide over “control” dishes.
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    URL: http://dx.doi.org/10.1007/BF00980076
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    Mutational analysis of the ‘conserved region’ of maize streak virus suggests its involvement in replication (1992)
    Springer
    Plant molecular biology 19 (1992), S. 601-610 
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    ISSN: 1573-5028
    Keywords: geminivirus ; agroinfection ; Zea mays ; large intergenic region (LIR)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Maize streak virus as well as other geminiviruses contain a potential hairpin structure with the conserved sequence TAATATTAC in the loop. We assessed the possible involvement of this structure in replication and symptom induction of the virus. A series of insertion and deletion mutants were analyzed by agroinfection. Deletion of the hairpin or insertions in the conserved sequence abolished symptom development. Viral DNA could not be detected in the infected tissue. However, a mutant with a point mutation in the ‘conserved’ sequence, isolated after inoculation of maize plants with an insertion mutant, was able to replicate and to induce symptoms.
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    An mRNA that specifically accumulates in maize roots delineates a novel subset of developing cortical cells (1992)
    Springer
    Plant molecular biology 20 (1992), S. 821-831 
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    ISSN: 1573-5028
    Keywords: cortex ; developmental regulation ; in situ hybridization ; organ-specific gene expression ; roots ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A near full-length cDNA clone (pZRP3) corresponding to an mRNA that accumulates specifically in roots of maize was isolated. The ZRP3 mRNA is ca. 600 nucleotides in length. The amino acid sequence of the predicted polypeptide is rich in leucine (16%), proline (11%), and cysteine (8.5%). The zrp3 gene appears to be expressed exclusively in roots, whereas other ZRP3-related genes are expressed in additional organs of the maize plant. In situ hybridization shows that ZRP3 mRNA accumulation is largely confined to the cells of the cortical ground meristem. Furthermore, accumulation of this mRNA occurs within a distinct subset of cortical cells, the inner three to four cell layers.
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    URL: http://dx.doi.org/10.1007/BF00027153
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    A DNA polymerase from maize axes: its purification and possible role (1992)
    Springer
    Plant molecular biology 20 (1992), S. 1159-1168 
    Details
    ISSN: 1573-5028
    Keywords: DNA polymerase ; germination ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Three different DNA polymerase activities can be resolved by passing a protein extract from 24 h imbibed maize axes through DEAE-cellulose. These activities have been numbered 1, 2 and 3, according to their elution order. One of them, DNA polymerase 2, elutes at 100–120 mM phosphates. This enzyme was further purified by passing it through Heparin-Sepharose, Sephacryl S-300 and DNA cellulose. Purification was nearly 5000-fold. The enzyme needs Mg2+, is stimulated by K+, has an optimum pH of 7.0 and its optimum temperature is 30–37 °C. Specific inhibitors for different types of polymerases, such as aphidicolin, dideoxythymidine triphosphate and N-ethyl maleimide, gave intermediate values of inhibition, making impossible the definition of the type of enzyme purified by its inhibitory pattern. SDS-PAGE indicated the presence of several bands of molecular masses of 28–40, 56 and 15 kDa. Most of these bands could be visualized when proteins from crude extracts were analyzed by western blot, using an antibody against calf thymus DNA polymerase α. A high molecular mass (around 500 kDa) was calculated by western blot of native gels using the same antibody. Finally, specific activity of this enzyme increased 100-fold during maize germination whereas polymerase 3 virtually did not increase. Furthermore, immunoprecipitation experiments with the antipolymerase α-antibody showed a decrease in DNA polymerase activity by 70%. The possibility that polymerase 2 is a replicative enzyme is discussed.
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    Stress responses in maize: Sequence analysis of cDNAs encoding glycine-rich proteins (1992)
    Springer
    Plant molecular biology 18 (1992), S. 847-849 
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    ISSN: 1573-5028
    Keywords: cDNA ; nucleotide sequence ; glycine-rich proteins ; chemical stress ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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    URL: http://dx.doi.org/10.1007/BF00020034
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    Multiple ocs-like elements required for efficient transcription of the mannopine synthase gene of T-DNA in maize protoplasts (1992)
    Springer
    Plant molecular biology 20 (1992), S. 219-233 
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    ISSN: 1573-5028
    Keywords: promoter ; electroporation ; protoplasts ; transient assay ; Agrobacterium ; Ti plasmid ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Regulatory elements controlling transcriptional activity of the mannopine synthase 2′ promoter (mas 2′) were defined by analysis of deletion mutants in transient expression assays in maize protoplasts. Deletion of the region between −305 and −290 containing sequence similarity to the octopine synthase (ocs) promoter element reduced activity by 67% compared to wild type activity. Less than 1% of the activity remained in 5′ deletions downstream of −153. Inclusion of various heterologous enhancer-like sequences immediately upstream of position −325 increased activity by up to 7.5-fold. Insertion of the −325 to −275 sequence alone, or in combination with heterologous enhancer-like elements, restored activity of some of the 5′-deletion mutants. Restoration of activity was not obtained with mutants deleted past position −127. Our results suggest that a single class of nuclear proteins from maize interact with high affinity at elements designated mas b (−306 to −275; mas 1′ element), d (−127 to −108), and e (−82 to −39; mas 2′ element) as well as the 20 bp element from the ocs promoter. Although the binding site at mas d only appears to accommodate a single protein, this element has the potential to make a weak, but positive, contribution to the activity of the mas 2′ promoter. The binding of nuclear proteins could not be demonstrated at mas a and c, both of which showed limited homology to the ocs element. Mutational evidence suggested that mas a and c may also contribute to mas 2′ transcription.
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    Nucleotide sequence of a region of maize chloroplast DNA containing the 3′ end of clpP, exon 1 of rps12 and rpl20 and their cotranscription (1992)
    Springer
    Plant molecular biology 18 (1992), S. 415-418 
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    ISSN: 1573-5028
    Keywords: ribosomal protein ; rps12 ; rpl20 ; clpP ; chloroplast genome ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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    URL: http://dx.doi.org/10.1007/BF00034970
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    Nitrate reductase transcript is expressed in the primary response of maize to environmental nitrate (1992)
    Springer
    Plant molecular biology 18 (1992), S. 55-64 
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    ISSN: 1573-5028
    Keywords: cycloheximide ; leaf ; nitrate induction ; nitrate reductase transcript ; root ; scutellum ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The nitrate induction of NADH:nitrate reductase mRNA in maize roots, scutella and leaves was investigated in the presence and absence of inhibitors of protein synthesis. In the absence of inhibitors, nitrate treatment caused a fairly rapid (2 to 3 h) increase in the level of the nitrate reductase transcript in all tissues. When cytoplasmic protein synthesis was inhibited by cycloheximide, nitrate reductase mRNA was induced by nitrate in all tissues to levels equal to or greater than those found with nitrate treatment alone. Treatment of maize tissues with cycloheximide in the absence of nitrate had only a small effect on the accumulation of the nitrate reductase mRNA. Inhibition of organellar protein synthesis with chloramphenicol also had little or no effect on nitrate-induced nitrate reductase mRNA accumuiation in roots and scutella, but did appear to partially inhibit appearance of transcript in leaves. Excision of scutella in the absence of nitrate was sufficient to cause some accumulation of the nitrate reductase transcript. Since cytoplasmic protein synthesis was not required for expression of nitrate reductase transcripts, induction of these transcripts by nitrate is a primary response of maize to this environmental signal. Thus, it appears that the signal transduction system mediating this response is constitutively expressed in roots, scutella and leaves of maize.
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    cDNA clones encoding Arabidopsis thaliana and Zea mays mitochondrial chaperonin HSP60 and gene expression during seed germination and heat shock (1992)
    Springer
    Plant molecular biology 18 (1992), S. 873-885 
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    ISSN: 1573-5028
    Keywords: Arabidopsis thaliana ; ATPase ; cpn60 ; developmental regulation ; molecular chaperones ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Mitochondria contain a nuclear-encoded heat shock protein, HSP60, which functions as a chaperonin in the post-translational assembly of multimeric proteins encoded by both nuclear and mitochondrial genes. We have isolated and sequenced full-length complementary DNAs coding for this mitochondrial chaperonin in Arabidopsis thaliana and Zea mays. Southern-blot analysis indicates the presence of a single hsp60 gene in the genome of A. thaliana. There is a high degree of homology at the predicted amino acid levels (43 to 60%) between plant HSP60s and their homologues in prokaryotes and other eukaryotes which indicates that these proteins must have similar evolutionarily conserved functions in all organisms. Northern- and western-blot analyses indicate that the expression of the hsp60 gene is developmentally regulated during seed germination. It is also heat-inducible. Developmental regulation of the (β-subunit) of F1-ATPase, an enzyme complex that is involved in the cyanide-sensitive mitochondrial electron transport system, indicates that imbibed embryos undergo rapid mitochondrial biogenesis through the early stages of germination. Based on the functional role of HSP60 in macromolecular assembly, these data collectively suggest that the presence of higher levels of HSP60 is necessary during active mitochondrial biogenesis, when the need for this protein is greatest in assisting the rapid assembly of the oligomeric protein structures.
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    Dissection of a pollen-specific promoter from maize by transient transformation assays (1992)
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    Plant molecular biology 18 (1992), S. 211-218 
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    ISSN: 1573-5028
    Keywords: pollen-specific gene expression ; promoter analysis ; transient assays ; Tradescantia ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have previously reported the isolation and characterization of a gene (Zm 13) from Zea mays which shows a pollen-specific pattern of expression. Stably transformed tobacco plants containing a reporter gene linked to portions of the Zm 13 5′ flanking region show correct temporal and spatial expression of the gene. Here we present a more detailed analysis of the 5′ regions responsible for expression in pollen by utilizing a transient expression system. Constructs containing the β-glucuronidase (GUS) gene under the control of various sized fragments of the Zm13 5′ flanking region were introduced into Tradescantia and Zea mays pollen via high-velocity microprojectile bombardment, and monitored both visually and with a fluorescence assay. The results suggest that sequences necessary for expression in pollen are present in a region from −100 to −54, while other sequences which amplify that expression reside between −260 and −100. The replacement of the normal terminator with a portion of the Zm13 3′ region containing the putative polyadenylation signal and site also increased GUS expression. While the −260 to −100 region contains sequences similar to other protein-binding domains reported for plants, the −100 to −54 region appears to contain no significant homology to other known promoter fragments which direct pollen-specific expression. The microprojectile bombardment of Tradescantia pollen appears to be a good test system for assaying maize and possibly other monocot promoter constructs for pollen expression.
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    Chromosomal and cell size analysis of cold tolerant maize (1992)
    Springer
    Theoretical and applied genetics 84 (1992), S. 798-802 
    Details
    ISSN: 1432-2242
    Keywords: Zea mays ; C-banding ; Cell size ; Chloroplast number ; Cold tolerance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary C-band number, guard cell length, and chloroplast number per guard cell were determined for eight maize populations. These populations consisted of maize selected for cold tolerance at the University of Nebraska as well as the original unselected populations. The genome size of these populations had previously been determined. C-band number fluctuated concertedly with the changes in genome size indicating that deletions and additions of constitutive heterochromatin occurred during selection, resulting in altered genome sizes. Guard cell size of all the cold tolerant populations was greater than the cell size of the respective nonselected populations. Chloroplast number per guard cell was also higher in all the cold tolerant populations than in their parental populations, but the increases were not statistically significant. The results indicate that changes in genome size that occurred during selection for cold tolerance are the result of changes in amounts of C-band heterochromatin and that the selection process results in an increase in cell size in the cold tolerant populations.
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    The structure of the SF6 molecule and the SF 6 − anion excited states (1992)
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    Russian chemical bulletin 41 (1992), S. 504-510 
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    ISSN: 1573-9171
    Keywords: electronic structure ; geometry optimization ; electron affinity ; stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The electronic and geometric structures of the ground state and a number of excited states of the SF6 molecule and the SF 6 − anion have been calculated by the discrete-variation method of the local density-functionals. The anion was found to possess a number of states stable toward the outer electron detachment, and at least one excited state stable toward dissociation. The adiabatic electron affinity (EA) was determined as 3.46 eV at the highest level of theory. This result is correlated to the high EAs of the isovalent compound SeF6 and TeF6; however, it does not agree with the presently accepted experimental estimate of 1.0 ± 0.2 eV for the SF6EA value. The basic anion configuration is octahedral with a S-F bond length of 1.717 Å. The calculated limit for the highest dissociation channel of the ground state SF 6 − → SF 5 − + F is ≈ 1.5 eV lower than the minimum of the total energy of the neutral molecule; this is in good agreement with experimental estimates.
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    Mechanical release and lectin labeling of maize root protoplasts (1992)
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    Protoplasma 169 (1992), S. 49-56 
    Details
    ISSN: 1615-6102
    Keywords: Lectin ; Plasma membrane ; Protoplast isolation ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An improved method for the mechanical release of protoplasts from plant tissues is described. The historically-low yield of mechanically-released protoplasts is greatly increased by use of a simple electrically-driven tissue sheer and by optimization of various other steps in the procedure. As counted by light microscopy of a purified preparation, the number of mechanically-released protoplasts obtained is about 6×104 per gram fresh weight of cortical tissue from the primary root of maize (Zea mays L. WF9×Mo 17) seedlings. Nuclear staining of the preparation, however, shows that about half of these protoplasts lack a nucleus and thus are actually subprotoplasts. Comparison of lectin binding to the plasma membranes of mechanically-and enzymatically-released protoplasts shows that both types contain binding sites forRicinus communis agglutinin. Binding sites for peanut (Arachis hypogaea) agglutinin are not naturally present on mechanically-released protoplasts but are generated by exposure to a mixture of Cellulysin and Pectolyase Y-23, the cell wall-degrading enzymes used to prepare enzymatically-released protoplasts.
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    Plant cell wall architecture is revealed by rapid-freezing and deep-etching (1992)
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    Protoplasma 167 (1992), S. 33-42 
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    ISSN: 1615-6102
    Keywords: Daucus carota ; Zea mays ; Vigna radiata ; Helicoidal cell walls ; Polylamellate cell walls ; Rapid-freeze deep-etch
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary This paper reports on preliminary investigations into the structure of cell walls of varying complexity as revealed by the rapidfreeze deep-etch technique. Three cell types from different species were examined in order to compare the three-dimensional arrangement of random, polylamellate and helicoidal walls. Each cell type displayed a distinctive level of organisation with respect to the cellulose microfibrils and the matrix material. In polylamellated walls, the microfibrils within each layer were linked to each other by 16–20 nm long side chains regularly spaced along the length of the microfibril. In helicoidal walls, the shifting of the microfibrils could cleary be seen, yet no recognisable structures were observed which could mediate this movement.
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    Effect of thefloury-2 locus on protein body formation during maize endosperm development (1992)
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    Protoplasma 171 (1992), S. 123-133 
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    ISSN: 1615-6102
    Keywords: Endosperm ; Floury-2 ; Immunocytochemistry ; Protein bodies ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The seed storage proteins of maize (Zea mays L.) are synthesized during endosperm development on membrane-bound polyribosomes. These proteins, collectively called zeins, are translocated into the lumen of the rough endoplasmic reticulum, where they assemble into protein bodies. Protein body formation in normal genotypes occurs via an ordered deposition of the various types of zeins, and leads to the formation of spherical structures with a diameter of about 1 μm. These structures consist of a central core that contains predominantly α-zein; this central region is surrounded by a peripheral layer of β- and γ-zeins, and the entire structure is bounded by rough endoplasmic reticulum. In the endosperm mutant floury-2 the levels of all classes of zeins are reduced; these kernels exhibit an opaque phenotype instead of the vitreous phenotype observed in normal genotypes. In contrast to the discrete, spherical protein bodies which are formed in normal maize endosperm, the protein bodies within floury-2 endosperm are irregular and the zeins are disorganized; patches of β- and γ-zeins occur within irregularly lobed clusters of α-zein within the lumen of the rough endoplasmic reticulum. The implications of this aberrant distribution are discussed, both with respect to protein body development and kernel characteristics.
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    Increases in binding protein (BiP) accompany changes in protein body morphology in three high-lysine mutants of maize (1992)
    Springer
    Protoplasma 171 (1992), S. 142-152 
    Details
    ISSN: 1615-6102
    Keywords: Endosperm ; Zea mays ; Immunolocalization ; Rough endoplasmic reticulum ; Binding protein ; Protein bodies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A maize 75 kDa protein recently has been identified as a plant homolog of the mammalian binding protein (BiP). To better understand the function of BiP in protein body formation in maize endosperm, immunomicroscopy studies were conducted on three maize endosperm mutants, floury-2, Mucronate, and Defective endosperm-B 30, in which the level of BiP is highly elevated. Our results showed that protein body morphology in all three mutants was altered. In addition, BiP was localized in both the ER and peripheral regions of the abnormal protein bodies. The degree to which protein body morphology differed from normal was positively correlated with increased amounts of BiP. In addition, the accumulation of BiP in abnormal protein bodies increased with protein body maturation. In the three endosperm mutants, the arrangement of zeins within protein bodies had been perturbed, yet none of the specific zein subclasses exhibited the staining pattern found for BiP. The association of BiP with abnormal packaging of proteins in protein bodies may reflect a biological function to mediate protein folding and assembly in maize endosperm.
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    The vibration and stability analysis of moderate thick plates by the method of lines (1992)
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    Applied mathematics and mechanics 13 (1992), S. 523-531 
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    ISSN: 1573-2754
    Keywords: moderate thick plate ; vibration ; stability ; method of lines
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Mathematics , Physics
    Notes: Abstract The method of lines based on Hu Hai-chang's theory for the vibration and stability of moderate thick plates is developed. The standard nonlinear ordinary differential equation (ODE) system for natural frequencies and critical load is given by use of ODE techniques, and then any indicated eigenvalue could be obtained directly from ODE solver by employing the so-called initial eigenfunction technique instead of the mode orthogonality condition. Numerical examples show that the present method is very effective and reliable.
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    Adaptive foraging by predators as a cause of predator-prey cycles (1992)
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    Evolutionary ecology 6 (1992), S. 56-72 
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    ISSN: 1573-8477
    Keywords: dynamics of adaptation ; optimal foraging ; predator-prey cycles ; stability ; type-2 functional response
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary When foraging has costs, it is generally adaptive for foragers to adjust their foraging effort in response to changes in the population density of their food. If effort decreases in response to increased food density, this can result in a ‘type-2’ functional response; intake rate increases in a negatively accelerated manner as prey density increases. Unlike other mechanisms for type-2 responses, adaptive foraging usually involves a timelag, because foraging behaviours do not often change instantaneously with changes in food density or risks. This paper investigates predator-prey models in which there are explicit dynamics for the rate of adaptive change. Models appropriate to both behavioural and evolutionary change are considered. Both types of change can produce cycles under similar circumstances, but under some evolutionary models there is not sufficient genetic variability for evolutionary change to produce cycles. If there is sufficient variability, the remaining conditions required for cycles are surprisingly insensitive to the nature of the adaptive process. A predator population that approaches the optimum foraging strategy very slowly usually produces cycles under similar conditions as does a very rapidly adapting population.
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    Reactivation of Mutator transposable elements of maize by ultraviolet light (1992)
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    Molecular genetics and genomics 234 (1992), S. 353-360 
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    ISSN: 1617-4623
    Keywords: Zea mays ; Somatic instability ; Bronze-2 ; Genomic shock ; Pollen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary After epigenetic loss of Mutator activity, the family of Mu elements in Zea mays becomes immobile and highly methylated; in addition, Mu9, the presumptive autonomous regulatory element, is transcriptionally silent and its copy number decreases in successive crosses to non-Mutator lines. Spontaneous reactivation, scored as restoration of somatic instability of potentially mutable alleles of Bronze-2, of such cryptic Mutator lines is rare, occurring with a frequency of about 10−4. Irradiation of pollen with 254 nm ultraviolet light increases reactivation rate in the progeny kernels by up to 40-fold. Accompanying reactivation, the copy number of Mu9 elements increased, two-fold in one line and 20 to 40-fold in a second line. Reactivation may involve direct DNA damage or immediate physiological stress in the treated pollen.
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    URL: http://dx.doi.org/10.1007/BF00538694
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    Regulated transcription of the maize Bronze-2 promoter in electroporated protoplasts requires the C1 and R gene products (1992)
    Springer
    Molecular genetics and genomics 233 (1992), S. 379-387 
    Details
    ISSN: 1617-4623
    Keywords: Bz2 gene ; Transcriptional regulation ; Anthocyanin pathway ; Transient assay ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The putative maize transcription factor genes R and C1 are required for expression of reporter genes with promoters from the Bz1 and A1 genes, which encode enzymes required for anthocyanin biosynthesis in maize. Bz2 is another anthocyanin biosynthetic gene; we show that expression of a reporter gene from the Bz2 promoter also requires R and C1 when the fusion construct is introduced into maize kernels by particle gun bombardment. When electroporated into maize protoplasts from a suspension cell line not synthesizing anthocyanins, reporter genes with Bz2, Bz1, and A1 promoters are expressed only when both R and C1 expression plasmids are co-electroporated. Electroporation of R and C1 expression plasmids also induces the endogenous genes required for anthocyanin synthesis, resulting in pink protoplasts within 24 h. RNase protection analysis demonstrates that accumulation of mRNA from the endogenous Bzl and Bz2 genes absolutely requires introduced R and C1. In time-course experiments there is a delay of 3–6 h before the Bz2 promoter is activated, supporting the proposed role for R- and C1-encoded proteins in transcriptional control. An excess of R relative to C1 suppresses expression of A1, Bz1, and Bz2 promoters, suggesting an interaction between the R and C1 proteins.
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    Mechanism of Ds1 excision from the genome of maize streak virus (1992)
    Springer
    Molecular genetics and genomics 233 (1992), S. 388-394 
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    ISSN: 1617-4623
    Keywords: Viral vector ; Transposable element ; Zea mays ; Agroinfection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have previously shown that the maize transposable element Ds1 introduced into maize plants by agroinfection can be excised from the genome of geminivirus maize streak virus (MSV). Excision depended strictly on the presence of an active Ac element in the plants. In this study, the excision products or “footprints” left in the MSV genome after Ds1 excision were extensively characterized and the effects of flanking sequences on Ds1 excision were analysed. Most types of footprints obtained were comparable to those described for Ds1 excision in the maize genome, and could be explained by the models proposed for excision of plant transposable elements. In two revertants, however, some terminal sequences of the Ds1 element were found to have been left behind at the excision site. The finding of this novel type of Ds1 footprint indicated that gene conversion events occurred during and/or after Ds1 excision from the MSV genome. A partial deletion of one copy of the 8 by duplications flanking the Ds1 element had no effect on the frequency or on the types of footprints of Ds1 excision from the MSV genome. Thus, the duplicated 8 by sequences flanking the transposable element are not involved in Ds1 excision. These results, as well as a statistical analysis of the modifications of the bases flanking the Ds1 element after excision, are discussed in terms of excision models.
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    Functional analysis of the — 300 region of maize zein genes (1992)
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    Molecular genetics and genomics 231 (1992), S. 369-374 
    Details
    ISSN: 1617-4623
    Keywords: Zea mays ; Transcription ; Transformation ; Endosperm ; Tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A 43 by fragment containing the — 300 region upstream control element common to the endosperm expressed zein genes of Zea mays L. has been analyzed by in vivo and in vitro techniques. Transient transformation studies with protoplasts from a maize endosperm culture indicate that the element positively affects CaMV 35S promoter-driven gene expression, and that this effect is both orientation- and position-dependent. Band-shift and Southwestern blotting experiments demonstrate that the element is specifically bound by different sets of DNA-binding proteins from seedling and endosperm nuclei. A 2 by substitution within the most conserved region of the element both reduces the stimulatory effect on transcription and alters the binding of nuclear proteins from both tissues.
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    URL: http://dx.doi.org/10.1007/BF00292705
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    Structural and functional analysis of theBz2 locus ofZea mays: characterization of overlapping transcripts (1992)
    Springer
    Molecular genetics and genomics 233 (1992), S. 269-277 
    Details
    ISSN: 1617-4623
    Keywords: Zea mays ; Bz2 locus ; Natural antisense transcripts ; Transposable elements ; Gene family
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Analysis of the transcription pattern of theBz2 locus revealed that overlapping transcripts are derived from opposite DNA strands. The most abundant transcript (sense transcript) has an open reading frame coding for a protein of 241 amino acids, whilst in the antisense orientation no open reading frame has been detected; the antisense transcripts are detected only in those tissues that show high levels of sense transcript. Particle gun experiments indicate that the sense transcript is sufficient to provide theBz2 function. The promoter driving the sense transcript contains the elements usually found in front of eukaryotic genes. In addition an element with similarity to theC1 andR binding sites identified in theBz1 promoter is found. Further upstream in the promoter region a transposon-like insertion has been identified. This element has features similar to members of theAc/Ds transposable element family. The putativeBz2 protein shows similarity to various other plant proteins and to anEscherichia coli protein. All related proteins have in common the fact that they are involved in stress responses.
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    In vivo demonstration of acid phosphatase activity in the rhizosphere of soil-grown plants (1992)
    Springer
    Plant and soil 144 (1992), S. 199-205 
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    ISSN: 1573-5036
    Keywords: acid phosphatase ; ectoenzymes ; naphthyl phosphate ; Picea abies ; rhizosphere ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract For in vivo demonstration of acid phosphatase activity in the rhizosphere of soil-grown plants filter papers were treated with a mixture of 1-naphthyl phosphate as substrate and the diazonium salt Fast Red TR as an indicator. After enzymatic hydrolysis, 1-naphthol forms a red complex with Fast Red TR. This method was applied to 8-day old maize plants and 3-year old Norway spruce plants growing in rhizoboxes in soil under non-sterile conditions. The treated filter paper is placed at the surface of roots and soil and acid phosphatase activity is visualized as a red-coloured ‘root print’ on the filter paper. The method can be used as a qualitative analysis of acid phosphatase in the rhizosphere. It also allows a rough estimate of phosphatase activity in different root zones.
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    URL: http://dx.doi.org/10.1007/BF00012876
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    Adaptation of barley (Hordeum vulgare L.) to harsh Mediterranean environments (1992)
    Springer
    Euphytica 62 (1992), S. 29-38 
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    ISSN: 1573-5060
    Keywords: adaptation ; barley ; drought ; heading date ; plant ideotype ; principal component analysis ; stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Barley adapted to the combined stresses of low winter temperatures and terminal drought requires a medium carly heading date, little affected by environmental fluctuations. Two plant ideotypes that are adapted to terminal drought in Mediterranean environments can be distinguished. The first combines early heading with good early vigour, erect winter growth habit, light plant colour, and ability to recover from cold damage. The second combines medium early heading, prostrate winter growth habit, dark winter plant colour which changes to pale green in spring, and cold tolerance. This study was conducted to determine the relationship between consistency in heading date and plant ideotype, and to identify the usefulness of earliness and plant ideotype as criteria for indirect selection for grain yield under drought. Thirty-six two-rowed entries of barley (Hordeum vulgare L.) were sown at 15 environments in northern Syria. Average grain yields ranged from 7 to 331 g/m2; the range in average heading date was 20 days. Consistency in date of heading was related to the second ideotype through vernalization requirement. Early heading was positively correlated with grain yield in most of the environments, but especially in low-yielding environments. After eliminating the effect of heading date, the second plant ideotype was advantageous only under harsh conditions. In early generations, selection under favourable conditions for earliness, prostrate winter growth habit, dark winter plant colour, and cold tolerance is a useful alternative for yield testing, to identify material well adapted to environments experiencing low winter temperatures and terminal drought stress. Selection for the first plant ideotype is proposed for Mediterranean environments with mild winters.
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    Divergent full-sib recurrent selection for germination at low temperature in a maize population (1992)
    Springer
    Euphytica 64 (1992), S. 21-29 
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    ISSN: 1573-5060
    Keywords: cold tolerance ; correlated responses ; germination ; kernel type ; kernel weight ; recurrent selection ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Four cycles of divergent full-sib recurrent selection for the ability to germinate at low temperature were conducted in a maize (Zea mays L.) F2 population. The selection criterion was the high (H) or the low (L) value in algebraic terms of the difference (DG) between germination percentage at 9.5°C (G9.5) detected 19 days after sowing and germination percentage at 25°C (G 25) seven days after sowing; both traits were evaluated in a controlled environment (germinator). Direct and correlated responses estimated during the course of selection were in accordance with those evaluated at the end. Selection for H led to populations with higher DG values, while the reverse was noted for L; differences between H and L populations increased in successive selection cycles, though divergence tended to level off. Selection for H also resulted in higher G 9.5 (day 19), shorter germination time and more flinty kernels, while selection for L led to responses in the opposite direction as well as to a lower G 9.5 detected 37 days after sowing (i.e. at the end of germination). In contrast, responses were negligible for G 25 and varied erratically from one cycle to another for kernel weight.
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    Evaluation of in vitro selection regimes for a mitochondrial trait (methomyl resistance) in cms-T maize (1992)
    Springer
    Plant cell, tissue and organ culture 28 (1992), S. 129-137 
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    ISSN: 1573-5044
    Keywords: cms-T ; in vitro selection ; methomyl resistance ; mitochondrial mutation ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Several factors affecting the success of selection in plant populations were examined for their relevance to in vitro selection. Three in vitro selection schemes and two growth assessment procedures were evaluated for effectiveness in selecting for a mitochondrial trait in maize: resistance to the insecticidal compound methomyl. Regenerable maize callus was derived from immature embryos of the three-way hybrid P39/IL766A2 x W182BN containing Texas male sterile cytoplasm (cms-T). Either low, gradually increasing, or high selection pressures were used to grow callus over a period of 3–5 months. There was no significant difference in recovery of resistant plants using these 3 methods. Growth of callus on medium containing methomyl was assessed by increase in fresh weight during the final month of selection or by increase in number of callus pieces over the course of selection. These quantitative measures of growth were unreliable indicators for gain in resistance within the callus population. A procedure for recovery of methomyl resistant and male-fertile cms-T plants is suggested.
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    Effects of pre-storage conditions on storage of in vitro cultures of Nephrolepis exaltata (L.) Schott and Cordyline fruticosa (L.) A. Chev. (1992)
    Springer
    Plant cell, tissue and organ culture 28 (1992), S. 167-174 
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    ISSN: 1573-5044
    Keywords: dark ; in vitro storage ; light ; stability ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In vitro cultures of Nephrolepis exaltata and Cordyline fruticosa were stored at 5°, 9° or 13°C, at a low irradiance (3–5 μmol m−2 s−1) or in darkness. Prior to storage the cultures were subjected to 18°, 21°, 24° or 27°C and 15, 30 or 45 μmol m−2 s−1 in a factorial combination. The optimal storage conditions for Nephrolepis were 9°C in complete darkness. These cultures were still transferable to a peat/perlite mixture at the end of the experimental period of 36 months. The optimal storage conditions for Cordyline were 13°C and a low light level (±3–5 μmol m-2 s-1). When the pre-storage conditions were normal growth room conditions (24°C and 30 μmol m-2 s-1), in vitro cultures could be stored for 18 months. With the most favourable pre-storage treatment (18°C and 15 μmol m-2 s-1) some cultures still had green shoots after 36 months of storage, but did not survive transfer to peat/perlite. Pre-conditioning before storage was most favourable for Nephrolepis, and not that important, but still favourable, for Cordyline. There was an interaction between pre-storage temperature and pre-storage irradiance. For both species a high irradiance level was less favourable than a low irradiance level when combined with high growth room temperatures.
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    Predictability of streamflow and particulate organic matter concentration as indicators of stability in prairie streams (1992)
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    Hydrobiologia 242 (1992), S. 7-18 
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    ISSN: 1573-5117
    Keywords: POM ; prairie streams ; predictability ; stability ; stream flow
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Predictability of stream discharge and particulate organic matter (POM) in the water column was estimated, using Colwell's indices of constancy and contingency, for 6 Texas prairie streams (1 each of 2nd, 3rd, and 4th order with intermittent or perennial discharge). Stream discharge in these 6 prairie streams varied between 0 and 36000 1 s−1, depending on the stream and season. Predictability (P) of discharge in these streams ranged from 0.45 to 0.62, within the range of values expected for North American streams. Predictability of stream discharge was not significantly different between streams. Particulate organic matter concentrations in these prairie streams are relatively low, ranging from 0.25 to 4.00 mg AFDM 1−1. Predictability of POM concentration in these streams was high, ranging from 0.75 to 0.85, and was largely the result of constancy of POM concentrations. Within the different POM size classes, Fine POM (FPOM) had the highest predictability (P = 0.89−0.96). In spite of relatively unpredictable stream discharge, POM remained fairly constant providing a measure of habitat predictability and stability.
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    Relationship of embryogenic competence in maize (Zea mays L.) leaves to mitotic activity, cell cycle and nuclear DNA content (1992)
    Springer
    Plant cell, tissue and organ culture 31 (1992), S. 215-221 
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    ISSN: 1573-5044
    Keywords: cell cycle ; DNA content ; embryogenic competence ; flow cytometry ; mitotic activity ; somatic embryogenesis ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Embryogenic competence of maize (Zea mays L.) leaf tissues was studied with regard to leaf age, distance from leaf base, mitotic activity, 2C nuclear DNA content, and the distribution of cells within the cell cycle. The highest embryogenic competence was observed in the first segment of the second innermost leaf. In a succession of 8 mm segments from the leaf base, the frequency of cells in the G0/G1 phase of the cell cycle and mitotic activity decreased with leaf age and with the distance from leaf base. No significant differences in 2C nuclear DNA content were found for leaf segments of different age and position. The mean 2C nuclear DNA content was 5.74 pg. The results suggested that the loss of embryogenic competence in mature maize leaf tissues is not related to changes in nuclear DNA content, and that the competence is not simply related to a certain distribution of cells within the cell cycle or to a certain level of mitotic activity.
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    Effect of ferulic acid on growth and hydrolytic enzyme activities of germinating maize seeds (1992)
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    Journal of chemical ecology 18 (1992), S. 1981-1990 
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    ISSN: 1573-1561
    Keywords: Allelopathy ; ferulic acid ; amylase ; maltase ; invertase ; acid protease ; acid phosphatase ; Zea mays ; growth ; root ; shoot
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Ferulic acid was tested for its effect on the growth of root and shoot, fresh and dry weights, and hydrolytic enzyme activities of germinating maize seeds. The results showed that root growth was inhibited more than shoot growth. A significant reduction in the activities of hydrolytic enzymes was also observed, which reflects a mechanism of action for the natural growth inhibitors, which may include other phenolic compounds.
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    Phenylacetic acid as a phytotoxic compound of corn pollen (1992)
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    Journal of chemical ecology 18 (1992), S. 897-905 
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    ISSN: 1573-1561
    Keywords: Pollen ; corn ; Zea mays ; teosinte ; Zea mexicana ; allelopathy ; phenylacetic acid ; phytotoxicity ; Amaranthus leucocarpus ; Echinochloa crusgalli
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Phenylacetic acid (PAA), one of the phytotoxic compounds in corn (Zea mays) pollen, was identified by GC-MS and by direct comparison with a pure commercial sample of PAA. Bioassays were carried out by testing whole pollen, methylene chloride extract of the pollen, and pure PAA on germination and radical growth ofAmaranthus leucocarpus andEchinochloa crusgalli. The effect of corn pollen was compared with that ofZea mexicana (Teosinte), one of the wild relatives of cultivated maize.
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    Lyapunov's direct method in the estimation of the Hausdorff dimension of attractors (1992)
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    Acta applicandae mathematicae 26 (1992), S. 1-60 
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    ISSN: 1572-9036
    Keywords: 58F13 ; Hausdorff measure ; Hausdorff dimension ; strange attractor ; Lorenz system ; Rössler system ; Lyapunov function ; stability ; chaos ; weakly contracting system ; monostability ; frequency theorem
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract This paper surveys results of the authors and others conceming estimates for the Hausdorff dimension of strange attractors, particularly in the case of (generalized) Lorenz systems and Rössler systems. A key idea is the interpretation of Hausdorff measure as an analogue of a Lyapunov function.
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    Chemical reactions as dynamical systems on the interval (1992)
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    Journal of statistical physics 66 (1992), S. 1557-1574 
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    ISSN: 1572-9613
    Keywords: Boltzmann ; chemical kinetics ; stability ; chaos ; entropy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract We consider the most general chemical reaction of the typen 1 A 1+...+n N A N ⇌m 1 B 1+...+m M B M whereN,M⩾1,n 1,...,n N andm 1,...,m M are positive integers defining the stoichiometry, andA 1,...,A N andB 1,...,B M are the names of chemicals or ions. We assume that ∑ i=1 N ni=∑ j=1 M mj. The time evolution of the concentrations is given by the law of mass action and leads to a dynamical system (with discrete or continuous time) which is governed by a polynomial map of the interval [B, C], where B⩾0 and C⩽1. We define the physically meaningful range for the parameters of the map, and we show that, within such a range, the map has a unique fixed point, which is stable and a global attractor, with the exception of one particular case, where bifurcation is observed.
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    Reconsidering the green revolution: Diversity and stability in cradle areas of crop domestication (1992)
    Springer
    Human ecology 20 (1992), S. 145-167 
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    ISSN: 1572-9915
    Keywords: agriculture ; development ; diversity ; stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Energy, Environment Protection, Nuclear Power Engineering , Ethnic Sciences
    Notes: Abstract This paper reconsiders two widely held hypotheses about the effects of the green revolution, that it led to biological simplification and instability. The hypothesis of biological simplification (genetic erosion) is tested with evidence from Andean agriculture, where farmers maintain a significant degree of crop diversity even as they adopt modern crop varieties. The hypothesis of increased instability is tested with evidence from Asia where wheat and rice yields show no general pattern of increased instability. Neither of these hypotheses is confirmed. The conventional wisdom about the green revolution should be reconsidered with emphasis on resilience and variation in modernizing farming systems.
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    Spatial and temporal trends in stream macroinvertebrate communities: the influence of catchment disturbance (1992)
    Springer
    Hydrobiologia 241 (1992), S. 173-194 
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    ISSN: 1573-5117
    Keywords: disturbance ; recovery ; fire ; streams ; stability ; macroinvertebrates ; watershed
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Macroinvertebrate communities of five headwater streams in catchments disturbed by wildfire were compared with five similar streams with no catchment disturbance. Over the five years of observation, communities in disturbed streams were more similar to one another than they were to reference streams. Communities in disturbed streams exhibited more year-to-year variation than reference streams, although some indication of decreasing variation was evident through time, and species richness was greater in reference streams than disturbed streams. No increasing trend in richness over time was observed in disturbed streams. Stability of the relative abundance structure and persistence of dominant taxa through time may be characteristic of temperate streams over moderate time intervals. Local effects of catchment-wide disturbance have persistent effects that alter these trends.
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    Cluster analysis of RFLP data from related maize inbred lines of the BSSS and LSC heterotic groups and comparison with pedigree data (1992)
    Springer
    Euphytica 60 (1992), S. 139-148 
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    ISSN: 1573-5060
    Keywords: coancestry coefficient ; genetic similarity ; maize ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary In this study, 31 elite inbred lines of maize (Zea mays L.) were analyzed with 149 clone-enzyme combinations for their respective RFLP profiles. Objectives were (1) to determine the utility of RFLPs for estimation of genetic similarties among 16 inbred lines from the Iowa Stiff Stalk Synthetic (BSSS) and among 15 inbred lines from the Lancaster Sure Crop (LSC) heterotic groups and (2) to compare genetic similarities based on molecular markers with those based on pedigree information. Coefficients of genetic similarity (GS) and coancestry (f) between pairs of lines from the same heterotic group were calculated from RFLP and pedigree data, respectively. For lines from the BSSS heterotic group, cluster analyses based on RFLP and pedigree data revealed similar associations. GS and f values were closely correlated (r=0.70) for related BSSS lines. For lines from the LSC heterotic group, considerable discrepancies existed between the GS and f values, especially for those pairs involving inbreds Va22 and Lo924. Effect of selection and/or erroneous pedigree records are discussed as possible explanations for the low correlation of GS and f values (r=0.07) for related LSC lines. RFLPs seem useful for investigation of relationships among maize inbreds, verification of pedigree records, and quantification of the degree of relatedness.
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    Structural observations during androgenic microspore culture of the 4c1 genotype of Zea mays L. (1992)
    Springer
    Euphytica 65 (1992), S. 61-69 
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    ISSN: 1573-5060
    Keywords: androgenesis ; in vitro culture ; maize ; microspores ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The capacity of the maize genotype 4c1 to regenerate microcalli and embryos from cultured microspores has been examined by comparing various cold pretreatments and culture media, using microspores and pollen at different stages of development. Viability of cultured cells was tested with FDA and their development was traced with light and fluorescence microscopy using DAPI as a nuclear dye. It was found that a pre-incubation of dissected flowers floating in a liquid nutrient medium at 8°C during 10–14 days was most successful for the induction of cell division. Among the developmental stages tested only the microspores appeared to regenerate. Subculture at 25°C in the same liquid medium, supplemented with 0.1 mg/l TIBA, gave highest rates of microspore division, i.e. up to 70% at 4 to 6 days of culture. All pathways described earlier for maize androgenic embryogenesis were observed within the 4c1 genotype. Symmetric divisions occurred in cultured microspores but most frequently asymmetric divisions lead to the formation of microcalli within 12 days of culture. In at least 60% of all dividing microspores cells were derived from the generative nucleus. Microcalli further developed either into loose or compact calli. Compact calli formed embryo-like structures.
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    Alteration of the structural attributes of a compact clay loam soil by growth of a perennial grass crop (1992)
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    Plant and soil 139 (1992), S. 195-202 
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    ISSN: 1573-5036
    Keywords: bulk density ; epoxy resin impregnation ; image analysis ; macroporosity ; nitrogen ; stability ; strength ; structure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Soil aggregate stability, organic matter content, pH, bulk density, strength, and macropore attributes were assessed in order to evaluate the influence of grass root growth in a field sward. The amount of grass grown was varied by varying the quantity of applied nitrogen fertilizer: following one year with a uniform application rate, nitrogen fertilizer was applied over the subsequent three years to a compact soil at zero (N0), moderate (N1) and high (N2) rates. Differences in herbage production were evident in the three years of the contrasting nitrogen treatments. An index of soil aggregate stability increased in response to the increased grass growth promoted by heavier applications of nitrogen, but both bulk density and vane shear strength were unchanged. Binary images of the soil solid and pore space showed that for each treatment the largest volume of macropores occurred close to the surface, particularly in N0 where there was more pore space than in either N1 or N2. Analysis of the pore structure attributes of the binary images revealed further differences between treatments, in particular, at 40–80 mm depth, the soil in treatment N0 had fewer and smaller pores, and greater distance between pores, than the soil in the N1 and N2 treatments. The larger macropore volume in N1 and N2 constituted a major portion of the air-filled porosity when the soil was relatively wet. It was concluded that the root growth in the intensively cropped grassland was conducive to maintenance of a relatively stable and porous soil structure. An attendant increase in soil acidity close to the soil surface was a disadvantage of the larger nitrogen inputs.
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    URL: http://dx.doi.org/10.1007/BF00009310
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    The impact of genotype x soil texture interaction on the efficiency of selection for yield in maize (Zea mays L.) (1992)
    Springer
    Euphytica 61 (1992), S. 61-65 
    Details
    ISSN: 1573-5060
    Keywords: population improvement ; maize ; Zea mays ; honeycumb selection ; adaptability ; stability ; yield
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary This study was undertaken to investigate the implications of genotype x soil texture interaction on response to selection in maize. Mass honeycomb selection for yield was applied for 11 cycles from the F2 of the single cross maize hybrid F68×NE2 in a field B with silty-clay-loam soil texture. Response to selection compared to the original single cross hybrid was estimated both in absence of competition and under solid stand in the selection field B and in a nearby field A differing in soil texture (clay-loam). A strong crossover type of interaction occurred both under solid stand and in the absence of competition in the two tests the improved population outyielded the hybrid in field B in the two densities, but lagged behing the hybrid in field A. The results suggest that interaction between genotype and soil texture might affect efficiency of selection detrimentally unless provision is taken for parallel selection early in the crop improvement program in fields differing in soil texture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00035547
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    Segregation of genetic markers among wheat doubled haploid lines derived from wheat x maize crosses (1992)
    Springer
    Euphytica 65 (1992), S. 145-152 
    Details
    ISSN: 1573-5060
    Keywords: doubled haploid ; genetic marker ; wheat ; wheat x maize crosses ; Triticum aestivum ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Wheat doubled haploid (DH) lines were produced from the F1 hybrid, Fukudo-komugi x Oligo Culm, through intergeneric crosses between wheat and maize. F2 plants and 203 DH lines were analyzed for the segregation of the eight genetic markers, namely, grain proteins, grain esterases, GA-insensitivity and glume traits. The segregation in the F2 plants fitted to the expected ratios. No deviation was observed among the DH lines, either, except for the glume pubescence. The result indicates the absence of correlation between the markers investigated and the efficiency of embryo formation in the DH lines.
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    URL: http://dx.doi.org/10.1007/BF00022576
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    Genetic improvement of cell-wall digestibility in forage maize (Zea mays L.). I. Performance of inbred lines and related hybrids (1992)
    Springer
    Euphytica 65 (1992), S. 187-194 
    Details
    ISSN: 1573-5060
    Keywords: forage maize ; Zea mays ; breeding ; nutritive value ; cell-wall digestibility ; stalk quality
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The current study deals with genetic improvement of the nutritive value of forage maize. In separate field trials, maize inbred lines without the brown midrib trait and derived hybrids were evaluated for stalk quality as well as some other agronomic traits. The aim was to relate the performance of lines and hybrids. Quality traits studied were the contents of ash and cell walls expressed as percentage of dry matter and the digestibilities of organic matter and cell walls (stalk-dv% and stalk-dcw%, respectively). The performance of hybrids was established in a trial at two locations with three replicates per location and the performance of lines at one location in an unreplicated trial. The range for stalk-dcw% was about 10 percentage units between hybrids and 15 percentage units between inbred lines. Stalk-dcw% had of all quality traits of hybrids the highest broad-sense heritability (h 2=0.74), and determined about 80% of the variation in stalk-dv%. The only stalk quality trait where a significant correlation was found between the mean hybrid performance and the corresponding midparent value was stalk-dcw% (r=0.70, P〈0.01). In conclusion, stalk-dcw% proved to be the only stalk quality trait worth evaluating at the inbred line level in a breeding programme aimed at producing commercial hybrid varieties of forage maize.
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    URL: http://dx.doi.org/10.1007/BF00023082
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    Variation among maize (Zea mays L.) accessions of Bendel State, Nigeria. Multivariate analysis of agronomic data (1992)
    Springer
    Euphytica 66 (1992), S. 65-71 
    Details
    ISSN: 1573-5060
    Keywords: cluster analysis ; principal component analysis ; accession ; landraces ; Zea mays ; maize ; dendrogram ; variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Two multivariate techniques were used to characterize 30 maize accessions collected from three ecological zones of Bendel State, Nigeria. Differentiation of the 30 accessions into five distinct groups was achieved with the unweighted variable group method of the average linkage cluster analysis of 34 agronomic characters. Four of the taxonomic groups contained at least three accessions each, while a fifth group contained only one. The single accession contained in the fifth group was characterised by very early maturity, deeply pigmented leaves and ear husks and short statured plants. Clustering of the accessions into different phenetic groups followed substantially along geographical and traditionally trading routes. A few cases of overlapping of accessions from different geographical locations were obtained. Principal component analysis revealed that days to 50% tasseling and silking, number of nodes/plant, ear length, ear weight, leaf width, and kernel colour were the principal discriminatory characters that differentiated the accessions. Sixty-four percent of the total variation among the 34 characters were accounted for by the first five principal components while the first and second components accounted for 26 and 14 respectively.
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    URL: http://dx.doi.org/10.1007/BF00023509
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    Adaptation and yield stability of selected lines of Lathyrus spp. under rainfed conditions in West Asia (1992)
    Springer
    Euphytica 66 (1992), S. 89-97 
    Details
    ISSN: 1573-5060
    Keywords: chickling ; Lathyrus ; G×E interactions ; adaptation ; stability ; heritability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Sixteen promising lines (selections) of Lathyrus spp. (chicklings), comprising 11 L. sativus, four L. ochrus, and one L. cicera, were evaluated under rainfed conditions in Syria. Two locations in each of four years were treated as separate environments, to give eight environments altogether. Genotype x environment (G×E) interactions were analysed using linear regression. There was considerable variation in herbage and seed yields within both lines and environments. Genotype x environment interactions were present for both herbage and seed yields; a little over half was accounted for by the linear regression. The non-linear component was also significant, although it was smaller than that of the linear component. The most stable herbage and seed yields were obtained from L. sativus. Two selections, 347 and 311, origmating from Syria and Turkey respectively, combined both high herbage and seed yields with wide adaptation and stability, and could be considered the most widely adapted lines. Other lines were identified as suitable for favourable and unfavourable environments; in particular, all four lines of L. ochrus have great potential in frost-free climates. The importance of genotype x environment interactions in future breeding strategies is discussed.
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    URL: http://dx.doi.org/10.1007/BF00023512
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    Differential effect of temperature on mitrochondrial activity in shoots from freshly harvested and moderately aged kernels of maize (Zea mays L.) (1992)
    Springer
    Plant growth regulation 11 (1992), S. 267-271 
    Details
    ISSN: 1573-5087
    Keywords: Mitochondria ; respiration ; seed ageing ; seed storage ; temperature ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This paper reports on a study of mitochondrial activity in etiolated shoots of freshly harvested and moderately aged kernels of maize. Activity was investigated after incubation at a favourable temperature (25°C), sub-optimal temperature (13°C) and after a heat shock (46°C for 2h). Although impaired mitochondrial activity in shoots from moderately aged maize kernels was not detected at 25°C, deficiencies became evident under low temperature stress (13°C). State 3 oxygen uptake, cyanide-insensitive oxygen uptake and cytochrome oxidase activity were lower in mitochondria from these shoots at 13°C than in mitochondria from shoots of freshly harvested kernels at this temperature. After a heat shock, cyanide-insensitive oxygen uptake was higher, and cytochrome oxidase activity lower, in shoots of aged kernels than in shoots of fresh kernels. No significant differences in ADP: O ratio or succinate dehydrogenase activity occurred between mitochondria from shoots of the two seed lots in any of the temperature treatments.
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    URL: http://dx.doi.org/10.1007/BF00024565
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    Degradation of Synthetic Salmon Calcitonin in Aqueous Solution (1992)
    Springer
    Pharmaceutical research 9 (1992), S. 1521-1523 
    Details
    ISSN: 1573-904X
    Keywords: salmon calcitonin ; stability ; kinetics ; peptide ; degradation ; pH–rate profile ; HPLC
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1015839719618
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    Effect of Surfactant Phase in Perfluorocarbon Emulsification Efficiency (1992)
    Springer
    Pharmaceutical research 9 (1992), S. 406-409 
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    ISSN: 1573-904X
    Keywords: centrifugation ; emulsification ; perfluorocarbon ; stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The effect of preparation temperature on the emulsification efficiency of perfluoro-3-butyltetrahydrofuran (FC-75) was investigated. Polyoxyethylene (POE) oleyl ether surfactants were used as the emulsifier(s) in a range of HLB values of 7.5 to 9.5. The emulsions were prepared by paddle mixing as a method of low-shear emulsification. After centrifugation of the resulting O/W emulsions, the volume of FC-75 which separated was utilized as a measure of the emulsification efficiency. In general, emulsions prepared at temperatures where the surfactant was in a lamellar-to-isotropic surfactant solution transition, Lα→ L3, displayed a better emulsification efficiency than those prepared with other surfactant phases.
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    The Influence of Sucrose, Dextran, and Hydroxypropyl-β-cyclodextrin as Lyoprotectants for a Freeze-Dried Mouse IgG2a Monoclonal Antibody (MN12) (1992)
    Springer
    Pharmaceutical research 9 (1992), S. 266-270 
    Details
    ISSN: 1573-904X
    Keywords: monoclonal antibody ; lyophilization ; freeze-drying ; stability ; formulation ; cyclodextrin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The influence of lyophilization on the stability of a monoclonal antibody (MN12) was investigated. MN12 was freeze-dried in different formulations [without lyoprotectant or in the presence of sucrose, dextran, or hydroxypropyl-β-cyclodextrin (HPβCD)] and under varying conditions (with or without secondary drying). Subsequently, the monoclonal antibody was stored for 18 or 32 days at various temperatures (4, 37, or 56°C). For comparison, solutions of MN12 were stored under the same conditions. Regardless of the lyoprotectant used, precipitation and a concomitant reduction of the antigen-binding capacity by about 10% were observed upon recon-stitution of lyophilized MN12. HPβCD proved to be the most effective stabilizer to prevent degradation of lyophilized MN12 during storage. Compared with MN12 solutions, HPβCD-containing lyophilized MN12 cakes were more resistant to heat-induced charge alterations and loss of antigen-binding capacity.
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    URL: http://dx.doi.org/10.1023/A:1018905927544
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    Application of Electrospray Mass Spectrometry (ES-MS) for the Analysis of Monoclonal Antibody Fc Subunits (1992)
    Springer
    Pharmaceutical research 9 (1992), S. 945-951 
    Details
    ISSN: 1573-904X
    Keywords: glycosylation ; mass Spectrometry ; monoclonal antibody ; protein characterization ; stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1015817403321
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    Analysis of Hydroxyurea in Capsules and Aqueous Solution and Stability Study with Capillary Gas Chromatography and Thermionic (N–P) Specific Detection (1992)
    Springer
    Pharmaceutical research 9 (1992), S. 115-118 
    Details
    ISSN: 1573-904X
    Keywords: capillary gas chromatography ; analysis ; stability ; urea derivative ; capsules ; mass spectrometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A method for the analysis of hydroxyurea (HU) in solutions, powder, or capsules by use of capillary gas chromatography with N–P thermionic specific detection is described. Upon injection of an HU solution in a methanol and acetone mixture, the drug formed pyridine which was well separated from the internal standard (thiotepa) on a 30-m fused-silica, SE-30 capillary column with temperature programming. The peak height ratio versus concentration standard curves were linear with correlation coefficient ranging between 0.9942 and 0.9993. The coefficients of variation at 5, 25, and 50 µg/L were 7.2, 5.7, and 5.5%, respectively. Hydroxyurea was extracted from powder or capsule formulations with a mixture of methanol and acetone (50:50, v:v), and the percentage found of the label claim for 10 capsules ranged between 96.7 and 104.9 (mean = 100.1; CV = 2.7%). Further, this assay was used to examine the stability of hydroxyurea in aqueous solutions at 4, 23, and 45°C, and the apparent first-order rate constants observed at these temperatures were 0.06407, 0.08113, and 0.1293 day-1, respectively; the activation energy was 3011 cal · K-1 mol-1.
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    URL: http://dx.doi.org/10.1023/A:1018996130753
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    An Electrically Modulated Drug Delivery Device. III. Factors Affecting Drug Stability During Electrophoresis (1992)
    Springer
    Pharmaceutical research 9 (1992), S. 312-315 
    Details
    ISSN: 1573-904X
    Keywords: controlled drug delivery system ; chronotherapeu-tics ; electrically modulated ; electrophoresis ; degradation of propranolol hydrochloride ; stability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A number of factors affecting the stability of propranolol HC1 during electrophoretic control were investigated. It was found that significant degradation of propranolol HC1 and hydrolysis of water occurred when a current of 15 mA was used with platinized electrodes. This degradation was thought to be due to decomposition of propranolol HC1 at the electrodes. Degradation could be significantly reduced by using uncoated platinum electrodes and currents in the range of 0 to 2.5 mA, while still allowing control of drug delivery rates. Electrode reaction processes were found at high ionic strengths and high drug concentrations but were not thought to be associated with drug decomposition.
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    Solid-State Stability Testing of Drugs by Isothermal Calorimetry (1992)
    Springer
    Pharmaceutical research 9 (1992), S. 939-944 
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    ISSN: 1573-904X
    Keywords: calorimetry ; microcalorimetry ; stability ; kinetics, solid state ; degradation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A new technique has been developed to calculate rapidly the solid-state room-temperature degradation rate of drugs and drug candidates. The technique utilizes measurements of the initial rate of heat output at several elevated temperatures by isothermal calorimetry and the degradation rate of the compound determined at a single elevated temperature by chromatography. The activation energies and degradation rates at 25°C calculated by conventional methods and by isothermal calorimetry are compared and discussed. The compounds studied were phenytoin, triamterene, digoxin, tetracycline, theophylline, diltiazem, and several proprietary ICI compounds.
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    URL: http://dx.doi.org/10.1023/A:1015865319250
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    Directional derivatives of optimal solutions in smooth nonlinear programming (1992)
    Springer
    Journal of optimization theory and applications 73 (1992), S. 27-45 
    Details
    ISSN: 1573-2878
    Keywords: Sensitivity ; stability ; nonlinear programming ; calmness
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract We consider a smooth nonlinear program subject to perturbations in the right-hand side of the constraints. We do not assume that the unique solution of the original problem satisfies any qualification hypothesis. We suppose instead that the direction of perturbation satisfies the hypothesis of Gollan. We study the variation of the cost and, with the help of some second-order sufficiency conditions, obtain some conditions satisfied by the first term of the expansion of the solution. These conditions may vary depending on the existence of a Lagrange multiplier for the original problem.
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    URL: http://dx.doi.org/10.1007/BF00940076
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    6-Methoxy-2-benzoxazolinone: A semiochemical for host location by western corn rootworm larvae (1992)
    Springer
    Journal of chemical ecology 18 (1992), S. 931-944 
    Details
    ISSN: 1573-1561
    Keywords: Diabrotica virgifera virgifera ; 6-methoxy-2-benzoxazolinone ; hydroxamic acids ; semiochemical ; attractants ; western corn rootworm ; host location ; Coleoptera ; Chrysomelidae ; Zea mays ; kairomone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A bioassay-driven sequential fractionation scheme was used to isolate all portions of a crude dichloromethane corn seedling extract behaviorally active to larvae of the western corn rootworm,Diabrotica virgifera virgifera LeConte. 6-Methoxy-2-benzoxazolinone (MBOA) was identified as one of the most important components of an attractive crude corn extract. MBOA was found on or in the intact root tissues by injecting an extract of undamaged roots onto an HPLC immediately after extraction. MBOA was demonstrated to be volatile and functions as a semiochemical in conjunction with carbon dioxide in host location by western corn rootworm larvae, which are oligophagous on the roots of maize and several other species of grasses. Because MBOA occurs almost exclusively in maize and other grasses, it offers a simple way for the larvae to distinguish possible hosts from non-hosts. MBOA has previously been reported as a chemical defense against other insect species. This is the first report in grasses of a secondary compound that is toxic or a deterrent to nonadapted insect herbivores but that is used as a semiochemical in host location by a specialist insect species.
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    Comparison of stability and control characteristics of two twin-lift helicopter configurations (1992)
    Springer
    Nonlinear dynamics 3 (1992), S. 199-223 
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    ISSN: 1573-269X
    Keywords: Twin-lift helicopter ; nonlinear model ; stability ; feedback linearization
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mathematics
    Notes: Abstract The stability and control characteristics of two twin-lift helicopter configurations are analyzed in this paper. In order to address the issue of configuration selection from a handling qualities viewpoint, their open-and closed-loop characteristics are compared. The two twin-lift configurations considered are the twin-lift with spreader bar and twin-lift without spreader bar. The nonlinear models describing the dynamics of these two configurations in the lateral/vertical plane are derived. The open-loop characteristics of the two systems are compared by linearizing the nonlinear models about a symmetric hovering equilibrium condition. The closed-loop characteristics of the two systems are compared using nonlinear controllers based on feedback linearization schemes. The performance of the resulting closed-loop systems in carrying out a typical twin-lift mission is evaluated through nonlinear simulation. Also, the effects of helicopter performance degradation and measurement errors on the overall system performance are discussed. [B] Matrix multiplying the control vector in the nonlinear model [B1] Matrix multiplying the control vector in the linear model [C] Matrix defining vector of variables to be controlled [C1] Damping matrix CijElement of the damping matrix e Parameter used in the linear model = M 1 h 1/I 1=M 2 h 2/I 2,/ft {f} Vector independent of controls in the nonlinear model g Acceleration due to gravity, ft/sec2 h1, h2Distance of tether attachment point to the center of gravity for helicopters 1 and 2, ft h Parameter used in the linear model, =h 1=h 2, ft h′ Distance between rotor hub and the helicopter center of gravity, ft h h/l′ H Distance of the load from the spreader bar c.g., ft H1, H2Length of tethers 1 and 2, ft IRMass moment of inertia of spreader bar, slug-ft2 I1, I2Roll moments of inertia of helicopters 1 and 2, slug-ft2 k′ Non-dimensional hub control moment coefficient KDDerivative gains KIIntegral gains KPProportional gains [Ki] Stiffness matrix KijElement of the stiffness matrix l′ Parameter used in the linear model, =H 1=H 2, ft L Spreader bar length, ft
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    On the stability of multi-layered fluids. Part I: analysis (1992)
    Springer
    Flow, turbulence and combustion 49 (1992), S. 307-334 
    Details
    ISSN: 1573-1987
    Keywords: stability ; linear-stability analysis ; multi-layered flow ; Orr-Sommerfeld equations ; interfacial conditions ; generalized eigenvalue problem ; air-water flow
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The linear stability of the Poiseuille flow of multi-layered different fluids, described mathematically by a system of Orr-Somerfeld differential equations, is investigated. A spectral method is used to rewrite this system into a generalized eigenvalue problem, which can be solved with the QZ-algorithm. Special attention is paid to the tractibility of the interfacial conditions of the stability problem. Since we will limit ourselves to a linear stability analysis, the analytical treatment of the interfacial conditions is simplified. Some results related to simple flow configurations are presented. The origin of certain regions of interfacial instability is explained by simple analytical reasoning.
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    Temporal relationships betweenZea mays, ostrinia nubilalis (Lep.: Pyralidae) and endophyticBeauveria bassiana (1992)
    Springer
    BioControl 37 (1992), S. 525-536 
    Details
    ISSN: 1573-8248
    Keywords: Beauveria bassiana ; Ostrinia nubilalis ; endophytic relationship ; biological control ; insect pathology ; Zea mays ; Beauveria bassiana ; Ostrinia nubilalis ; lutte biologique ; relation endophyte ; pathologie de l'insecte ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Résumé Le champignon entomopathogène,Beauveria bassiana (Balsamo) Vuillemin a été épandu sur maïs, au stade cornet (V7) par application foliaire d'une formulation granulée de grits de maïs contenant des conidies ou par injection d'une suspension de conidies. Toutes les plantes ont été infestées à l'aide de larves de la Pyrale du maïs,Ostrinia nubilalis, au stade V7 (cornet), V12 (fin de stade cornet), ou V17 (apparition de la panicule). Les plantes infestées au stade cornet et à la fin du stade cornet ont eu significativement plus de chenilles de Pyrale en train de miner que les plantes infestées au stade début de panicule mâle. Le pourcentage de plantes infestées parB. bassiana n'était pas significativement différent entre ces 3 stades phénologiques. Au fur et à mesure que les plantes se développaient,B. bassiana était isolé de différentes parties de la plante, la moelle étant plus souvent infestée que les ligules. Les applications foliaires deB. bassiana ont entraîné la destruction immédiate de la pyrale chez les plantes infestées au stade cornet. La baisse de l'efficacité deB. bassiana aux stades intermédiaires par comparaison avec son efficacité au moment de la récolte est discutée.
    Notes: Abstract The entomopathogenic fungus,Beauveria bassiana (Balsamo) Vuillemin, was applied to whorl-stage (V7) corn,Zea mays L., by foliar application of a granular formulation of corn grits containing conidia or by injection of a conidial suspension. All plants were infested with European corn borer larvae,Ostrinia nubilalis (Hübner), at the V7 (whorl), V12 (late-whorl), or V17 (pretassel) stage of plant development. Plants infested at whorl and late-whorl stages had significantly more European corn borer tunneling than did plants infested at the pretassel stage. The percentage of plants colonized byB. bassiana did not differ significantly among the whorl, late-whorl, and pretassel stages. As the plants matured,B. bassiana was isolated from different plant areas, with the pith more frequently colonized than the leaf collars. Foliar application ofB. bassiana provided immediate suppression ofO. nubilalis in those plants infested at whorl stage. The reduced efficacy ofB. bassiana at the intermediate plant stages relative to efficacy at harvest is discussed.
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    URL: http://dx.doi.org/10.1007/BF02372322
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