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  • Articles  (33)
  • Cells, Cultured  (33)
  • 1995-1999
  • 1980-1984  (33)
  • 1935-1939
  • 1930-1934
  • 1920-1924
  • 1984  (33)
  • Physics  (33)
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  • Articles  (33)
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  • 1995-1999
  • 1980-1984  (33)
  • 1935-1939
  • 1930-1934
  • 1920-1924
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  • 1
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    Heterogeneity of normal human diploid fibroblasts: isolation and characterization of one phenotype (1984)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1984-01-13
    Description: Cultures of human diploid fibroblasts contain cells that respond to exposure to the first component of complement (C1) by initiating DNA synthesis and growth. The plasma membranes of these cells have specific binding sites for the C1q subcomponent of C1. A fluorescence-activated cell sorter was used to isolate a subset of cells with a high affinity for C1q, and the growth and synthesis activities of these high-affinity cells were studied after numerous replications in vitro. These cells synthesize DNA and grow faster than the parent cultures and low-affinity cells, and they produce two to three times as much protein. About 40 percent of their total protein synthesis activity is directed to collagen production, unusually high proportions of collagen types III and V being produced. These properties and the high affinity of the cells for C1q are retained for at least six cell transfers. This phenotype has the properties expected of fibroblasts in healing wounds and inflamed tissues.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Bordin, S -- Page, R C -- Narayanan, A S -- DE-02600/DE/NIDCR NIH HHS/ -- DE-03301/DE/NIDCR NIH HHS/ -- New York, N.Y. -- Science. 1984 Jan 13;223(4632):171-3.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6691142" target="_blank"〉PubMed〈/a〉
    Keywords: *Antigens, CD44 ; Carrier Proteins ; Cell Division ; Cell Separation ; Cells, Cultured ; Collagen/*biosynthesis/classification ; DNA/*biosynthesis ; Fibroblasts/analysis/cytology/*physiology ; Flow Cytometry ; Gingiva ; Humans ; *Membrane Glycoproteins ; Mitochondrial Proteins ; Phenotype ; *Protein Biosynthesis ; Receptors, Complement/*analysis
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
    Published by American Association for the Advancement of Science (AAAS)
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  • 2
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    A novel nuclear form of estradiol receptor in MCF-7 human breast cancer cells (1984)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1984-09-14
    Description: Nuclear estrogen receptor from MCF-7 cells undergoes a time-dependent, hormone-inducible transformation to a form that is less extractable from nuclei and less exchangeable with ligand. This receptor-modifying, intranuclear event is independent of receptor loss (processing) and appears associated with hormone responsiveness (progesterone-receptor induction) in these cells. The magnitude of receptor loss, however, is variable and apparently not a prerequisite for hormone action to induce progesterone receptor.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Kasid, A -- Strobl, J S -- Huff, K -- Greene, G L -- Lippman, M E -- New York, N.Y. -- Science. 1984 Sep 14;225(4667):1162-5.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6474170" target="_blank"〉PubMed〈/a〉
    Keywords: Breast Neoplasms/*metabolism ; Cell Nucleus/metabolism ; Cells, Cultured ; Female ; Humans ; Receptors, Estradiol ; Receptors, Estrogen/*metabolism ; Receptors, Progesterone/biosynthesis ; Time Factors
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 3
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    Frequent detection and isolation of cytopathic retroviruses (HTLV-III) from patients with AIDS and at risk for AIDS (1984)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1984-05-04
    Description: Peripheral blood lymphocytes from patients with the acquired immunodeficiency syndrome (AIDS) or with signs or symptoms that frequently precede AIDS (pre-AIDS) were grown in vitro with added T-cell growth factor and assayed for the expression and release of human T-lymphotropic retroviruses (HTLV). Retroviruses belonging to the HTLV family and collectively designated HTLV-III were isolated from a total of 48 subjects including 18 of 21 patients wih pre-AIDS, three of four clinically normal mothers of juveniles with AIDS, 26 of 72 adult and juvenile patients with AIDS, and from one of 22 normal male homosexual subjects. No HTLV-III was detected in or isolated from 115 normal heterosexual subjects. The number of HTLV-III isolates reported here underestimates the true prevalence of the virus since many specimens were received in unsatisfactory condition. Other data show that serum samples from a high proportion of AIDS patients contain antibodies to HTLV-III. That these new isolates are members of the HTLV family but differ from the previous isolates known as HTLV-I and HTLV-II is indicated by their morphological, biological, and immunological characteristics. These results and those reported elsewhere in this issue suggest that HTLV-III may be the primary cause of AIDS.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Gallo, R C -- Salahuddin, S Z -- Popovic, M -- Shearer, G M -- Kaplan, M -- Haynes, B F -- Palker, T J -- Redfield, R -- Oleske, J -- Safai, B -- New York, N.Y. -- Science. 1984 May 4;224(4648):500-3.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6200936" target="_blank"〉PubMed〈/a〉
    Keywords: Acquired Immunodeficiency Syndrome/blood/*microbiology ; Adult ; Antigens, Viral/analysis ; Cells, Cultured ; Cytopathogenic Effect, Viral ; Deltaretrovirus/*isolation & purification/physiology/ultrastructure ; Female ; Homosexuality ; Humans ; Immune Sera/pharmacology ; Interferon Type I/immunology ; Male ; RNA-Directed DNA Polymerase/metabolism ; Risk ; T-Lymphocytes/microbiology
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 4
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    Isolation and culture of a tetraploid subpopulation of smooth muscle cells from the normal rat aorta (1984)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1984-11-02
    Description: Smooth muscle cells with 4C (double diploid) DNA content have been found in major arteries. The proportion of 4C cells increases with normal aging and with hypertension. These cells may represent a state of arrest at the G2 phase of the cell cycle or may be examples of true tetraploidy. Flow cytometric cell sorting was used to isolate 4C smooth muscle cells from the rat aorta, and the cells were cultured. Flow cytometry, Feulgen microdensitometry, and karyotyping of the progeny of the 4C cells established the presence of true tetraploid cells. These findings demonstrate the presence of reproductively viable tetraploid cells in a normal mammalian tissue.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Goldberg, I D -- Rosen, E M -- Shapiro, H M -- Zoller, L C -- Myrick, K -- Levenson, S E -- Christenson, L -- 5-P01-CA-12662/CA/NCI NIH HHS/ -- AG00599/AG/NIA NIH HHS/ -- New York, N.Y. -- Science. 1984 Nov 2;226(4674):559-61.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6494901" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Aorta, Thoracic/analysis/*cytology ; Cells, Cultured ; DNA/analysis ; Flow Cytometry ; Humans ; Karyotyping ; Muscle, Smooth, Vascular/analysis/*cytology ; *Polyploidy ; Rats ; Rats, Inbred Strains
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 5
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    Reversal of knob formation on Plasmodium falciparum-infected erythrocytes (1984)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1984-10-05
    Description: The human malarial parasite Plasmodium falciparum can produce surface protrusions (knobs) on infected erythrocytes; however, long-term culturing of the parasite results in the appearance of knobless cells. In this study it was found that a knob-producing clone lost the ability to produce knobs in vitro. Furthermore, a clone not producing knobs derived from the knob-producing clone regained the capacity to produce knobby cells in vitro. Certain parasite proteins were associated with the knobby phenotype but not with the knobless type. These results indicate that the parasites change in vitro in a spontaneous and reversible manner independent of immunological selection.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Gritzmacher, C A -- Reese, R T -- AI 18695/AI/NIAID NIH HHS/ -- DRR 00833/RR/NCRR NIH HHS/ -- New York, N.Y. -- Science. 1984 Oct 5;226(4670):65-7.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6382613" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Cells, Cultured ; Clone Cells ; Erythrocytes/*parasitology/ultrastructure ; Humans ; Mutation ; Phenotype ; Plasmodium falciparum/analysis/genetics/growth & development/*physiology ; Proteins/analysis
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 6
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    Serotonin selectively inhibits growth cone motility and synaptogenesis of specific identified neurons (1984)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1984-11-02
    Description: The motile activity of growth cones of specific identified neurons is inhibited by the neurotransmitter serotonin, although other identified neurons are unaffected. As a consequence, affected neurons are unable to form electrical synapses, whereas other neurons whose growth is unaffected can still interconnect. This result demonstrates that neurotransmitters can play a prominent role in regulating neuronal architecture and connectivity in addition to their classical role in neurotransmission.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Haydon, P G -- McCobb, D P -- Kater, S B -- HD18577/HD/NICHD NIH HHS/ -- NS15350/NS/NINDS NIH HHS/ -- NS18819/NS/NINDS NIH HHS/ -- New York, N.Y. -- Science. 1984 Nov 2;226(4674):561-4.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6093252" target="_blank"〉PubMed〈/a〉
    Keywords: Animal Population Groups ; Animals ; Cells, Cultured ; Neurons/*drug effects/growth & development ; Serotonin/*pharmacology ; Snails ; Synapses/*drug effects ; Synaptic Transmission
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 7
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    Monoclonal antibody to Thy-1 enhances regeneration of processes by rat retinal ganglion cells in culture (1984)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1984-04-20
    Description: Ganglion cells were dissociated from postnatal rat retinas, identified by specific fluorescent labels, and maintained in culture on a variety of substrates. Regeneration of processes by retinal ganglion cells was enhanced when the cells were plated on glass coated with a monoclonal antibody against the Thy-1 determinant. Plain glass and glass coated with polylysine, collagen, fibronectin, or other monoclonal antibodies supported the growth of neural processes, but were less effective than antibody to Thy-1.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Leifer, D -- Lipton, S A -- Barnstable, C J -- Masland, R H -- EY01075/EY/NEI NIH HHS/ -- EY03735/EY/NEI NIH HHS/ -- EY04179/EY/NEI NIH HHS/ -- New York, N.Y. -- Science. 1984 Apr 20;224(4646):303-6.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6143400" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Antibodies, Monoclonal/*physiology ; Antigens, Surface/*immunology ; Antigens, Thy-1 ; Cell Adhesion ; Cells, Cultured ; Isoantibodies/*physiology ; *Nerve Regeneration ; Polylysine/pharmacology ; Rats ; Retina/cytology/*physiology ; Retinal Ganglion Cells/*physiology
    Print ISSN: 0036-8075
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    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 8
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    Isolation of lymphocytopathic retroviruses from San Francisco patients with AIDS (1984)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1984-08-24
    Description: Infectious retroviruses have been detected in 22 of 45 randomly selected patients with acquired immune deficiency syndrome (AIDS) and in other individuals from San Francisco. The AIDS-associated retroviruses (ARV) studied in detail had a type D morphology, Mg2+-dependent reverse transcriptase, and cytopathic effects on lymphocytes. The viruses can be propagated in an established adult human T cell line, HUT-78. They cross-react with antiserum to the lymphadenopathy-associated retrovirus isolated from AIDS patients in France. Antibodies to ARV were found in all 86 AIDS patients and in a high percentage of 88 other homosexual men in San Francisco. This observation indicates the widespread presence of these lymphocytopathic retroviruses and their close association with AIDS.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Levy, J A -- Hoffman, A D -- Kramer, S M -- Landis, J A -- Shimabukuro, J M -- Oshiro, L S -- CA-34980/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1984 Aug 24;225(4664):840-2.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6206563" target="_blank"〉PubMed〈/a〉
    Keywords: Acquired Immunodeficiency Syndrome/immunology/*microbiology ; Antibodies, Viral/analysis ; Bone Marrow/microbiology ; California ; Cell Line ; Cells, Cultured ; Cross Reactions ; Cytopathogenic Effect, Viral ; Deltaretrovirus/immunology/*isolation & purification/physiology/ultrastructure ; *Homosexuality ; Humans ; Leukocytes/microbiology ; Lymphatic Diseases/immunology ; Male ; RNA-Directed DNA Polymerase/metabolism ; Syndrome ; T-Lymphocytes ; Virus Cultivation
    Print ISSN: 0036-8075
    Electronic ISSN: 1095-9203
    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 9
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    Time-varying magnetic fields: effect on DNA synthesis (1984)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1984-02-24
    Description: Human fibroblasts have exhibited enhanced DNA synthesis when exposed to sinusoidally varying magnetic fields for a wide range of frequencies (15 hertz to 4 kilohertz) and amplitudes (2.3 X 10(-6) to 5.6 X 10(-4) tesla). This effect, which is at maximum during the middle of the S phase of the cell cycle, appears to be independent of the time derivative of the magnetic field, suggesting an underlying mechanism other than Faraday's law. The threshold is estimated to be between 0.5 X 10(-5) and 2.5 X 10(-5) tesla per second. These results bring into question the allegedly specific magnetic wave shapes now used in therapeutic devices for bone nonunion. The range of magnetic field amplitudes tested encompass the geomagnetic field, suggesting the possibility of mutagenic interactions directly arising from short-term changes in the earth's field.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Liboff, A R -- Williams, T Jr -- Strong, D M -- Wistar, R Jr -- New York, N.Y. -- Science. 1984 Feb 24;223(4638):818-20.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6695183" target="_blank"〉PubMed〈/a〉
    Keywords: Cells, Cultured ; DNA/*biosynthesis ; Humans ; *Magnetics ; Mutation ; Periodicity
    Print ISSN: 0036-8075
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    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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  • 10
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    Sequential interaction of glia maturation factor with insulin (1984)
    American Association for the Advancement of Science (AAAS)
    Details
    Publication Date: 1984-03-30
    Description: Astroblasts in culture proliferated when exposed to glia maturation factor for at least 2 hours and then to insulin, but not when exposed in the reverse order. The sequential relation suggests that glia maturation factor is a competence factor.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Lim, R -- Miller, J F -- CA-31796/CA/NCI NIH HHS/ -- New York, N.Y. -- Science. 1984 Mar 30;223(4643):1419-20.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/6367047" target="_blank"〉PubMed〈/a〉
    Keywords: Animals ; Astrocytes/drug effects/physiology ; Cell Division/drug effects ; Cells, Cultured ; Drug Interactions ; Glia Maturation Factor ; Growth Substances/*pharmacology/physiology ; Insulin/*pharmacology/physiology ; Mice ; Mice, Inbred BALB C ; Nerve Tissue Proteins/*pharmacology/physiology ; Rats
    Print ISSN: 0036-8075
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    Topics: Biology , Chemistry and Pharmacology , Computer Science , Medicine , Natural Sciences in General , Physics
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