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  • 1
    ISSN: 1570-7458
    Keywords: Pterostichus melanarius ; Coleoptera ; Carabidae ; pitfall traps ; insecticides ; prey ; hunger ; activity ; Rhopalosiphum padi ; Fenitrothion ; Sumicidin (Fenvalerate) ; spring barley
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Au cours d'essais à grande échelle, effectués en 1981 et 1982, sur orge de printemps, pour évaleur l'effet des insecticides Fenitrothion et Sumicidine (fenvalerate) sur les arthropodes utiles, un accroissement significatif des captures, dans des trappes pièges de Barber, de Pterostichus melanarius (Illiger) a été observé plusierus semaines après le traitement dans les lots traités par rapport aux lots témoins. Significativement plus de femelles de P. melanarius ont été capturées dans les parcelles traitées pendant cette période d'accroissement après traitement. Une diminution correspondante dans les populations de proies a été observée à la suite des traitements. Chaque femelle de P. melanarius des parcelles traitées avait une fraction significativement moins importance de son intestin remplie d'aliments solides (arthropodes) que celles des parcelles non traitées. Des auteurs précédents avaient observé des taux de capture plus rapides dans les trappes et ne plus grande mobilité des carabes affamés. La réduction des populations proies par l'application d'insecticides affamerait les carabes dont l'activité serait par suite plus élevée. Comme le taux de captures dans les trappes est dû à la fois à la taille de la population et à son activité, on en déduit que la capture par trappes seule ne peut donner une image exacte de l'effet des insecticides sur les populations de carabes dans les champs.
    Notes: Abstract During large-scale field experiments in 1981 and 1982, designed to assess the effects of the insecticides Fenitrothion and Sumicidin (Fenvalerate) on beneficial arthropods in spring barley, significant increases in pitfall-trap catches of Pterostichus melanarius (Illiger) were observed in treated plots compared to untreated controls, several weeks after treatment. Significantly more female P. melanarius were caught in treated plots during these post-treatment increases. Corresponding decreases were observed in prey populations following treatments. Individual female P. melanarius from treated plots had significantly fewer of their gut areas full of solid (arthropod) food when compared to those from untreated control plots. Earlier workers observed both faster recapture rates in pitfalls and higher mobility in hungry carabid beetles. The following hypothesis is suggested: reducing prey populations by the application of insecticides results in hungrier carabid beetles with consequently higher activity. Since pitfall-trap catch is determined not only by population size but also activity, it is argued that pitfall-trap catches alone cannot give a true measure of the effects of insecticides on carabid populations in the field.
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  • 2
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    Entomologia experimentalis et applicata 34 (1983), S. 291-296 
    ISSN: 1570-7458
    Keywords: Coleoptera ; Curculionidae ; Cyrtobagous ; Salvinia weevil ; Feeding characteristics of larvae ; Development of larvae ; Plant nutrition ; Temperature effects on development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Après leur naissance, les larves de Cyrtobagous sp. passent de 1 à 4 jours à brouter les racines, et de 0 à 9 jours dans les bourgeons de la plante hôte, Salvinia molesta, avant d'y creuser une galerie dans le rhizome. Bien que les larves aient été capables de survivre jusqu'à 38 jours en broutant les racines, quant elles furent privées de rhizome, elle ne purent achever leur développement. Quand les galeries furent commencées, le développement larvaire, entre 21° et 31°, dépendit de la température et de la teneur en azote de l'hôte. Les larves ne se développèrent pas à 17°. Une haute valeur nutritive de l'aliment consommé par les larves réduisit la durée du développement, mais n'influença pas la durée ultérieure de leur développement nymphal. Les lieux de prise de nourriture et les dégâts produits par des larves isolées furent précisés.
    Notes: Abstract Newly-emerged larvae of a salvinia weevil, Cyrtobagous sp. spent from 1–4 days browsing on the roots and from 0–9 days in the buds of the host plant, Salvinia molesta, prior to tunnelling into the rhizome of this aquatic weed. Although larvae were able to survive up to 38 days browsing on roots when rhizomes were withheld, they were unable to complete development. After tunnelling began, larval development between 21° and 31° was dependent on temperature and nitrogen levels in the host. Larvae failed to develop at 17°. High nutritional intake by the larvae reduced larval development time but did not influence duration of their subsequent pupal development. The feeding sites and plant damage produced by individual larvae were assessed.
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  • 3
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    Entomologia experimentalis et applicata 34 (1983), S. 27-34 
    ISSN: 1570-7458
    Keywords: Hymenoptera ; Aphidiidae ; Aphidius rhopalosiphi ; Sitobion avenae ; Functional response
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Nous avons examiné quelques aspects des relations entre le parasitoïde Aphidius rhopalosiphi et son hôte Sitobion avenae. La fécondité potentielle à l'émergence (144 œufs) était inférieure à la fécondité “réalisée”: nombre de pucerons momifiés (212). La plupart des œufs étaient pondus dans la première semaine de la vie imaginale (fig. 1). Le super-parasi-tisme s'est produit dans les conditions expérimentales, mais la distribution des larves (fig. 2) s'écartait souvent de la distribution au hasard, ce qui indiquait une certaine discrimination parmi les hôtes. La longévité moyenne était de 13,1 jours et le maximum de 23 jours. Le taux sexuel (fig. 3) pour l'ensemble de la descendance était 1/1, mais changeait dans le temps. Après le premier jour de ponte, plus de femelles que de mâles étaient obtenues; mais après 6 jours, il n'y avait presque plus que des mâles. Le taux de rencontre des aphides et des parasitoïdes augmentait avec la taille (fig. 4a). Le temps de prospection de l'hôte était en moyenne de 2 secondes, mais dans certains cas il atteignait 10 secondes ou plus. La proportion de rencontres efficaces était plus faible au 4ème stade (fig. 4b). Il n'y avait pas de différence significative dans le nombre de larves de parasitoïdes obtenues par rencontre pendant les stades 1 à 3 des pucerons, mais elle était significativement inférieure au 4ème stade par rapport au second. Les taux globaux de succès (taux de rencontre et nombre de larves formées par rencontre) étaient plus élevés aux 2ème stades des pucerons. La réponse fonctionnelle était sigmoïde (fig. 5).
    Notes: Summary Aphidius rhopalosiphi produced 212 offspring on average but in one case 509 deposited eggs were found. Superparasitization occurred but the frequency distribution of parasitoid larvae in aphids differed significantly from random, indicating a certain degree of host discrimination. Average life span of adults was 13.1 days and sex ratio was 1:1. It changed in time among successively produced offspring. Handling time was about 2 sec and was somewhat longer in the fourth instar than in the second. Second and third aphid instars were preferred for oviposition. Functional response was sigmoid and at an aphid density of 100 aphids per cage percentage parasitization decreased.
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  • 4
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    Entomologia experimentalis et applicata 34 (1983), S. 78-84 
    ISSN: 1570-7458
    Keywords: Hymenoptera ; Braconidae ; Microplitis croceipes ; Heliothis ; Parasitoid ; Host suitability ; Host selection ; 3-trophic level interactions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung Microplitis croceipes (Cresson), Parasit der Heliothis-Larven, wurde in 9 Kombinationen von Wirtsinsekt/Wirtspflanze gezüchtet. Im allgemeinen war das Ueberleben der Parasiten (1) besser in Wirtsraupen von Baumwolle als von Bohnen oder Tomaten, (2) besser in H. zea als in H. virescens und (3) besser in laborgezüchteten H. zea als in Raupen aus dem Freiland. Mehrere Versuche über die Eiablagepräferenz der Parasiten zeigten, dass mehr Eier in Raupen von Baumwolle als in Raupen von Bohnen oder gar von Tomaten abgelegt wurden. Die Wespen unterschieden aber nicht zwischen H. zea und H. virescens. Die Eiablagepräferenzen waren unabhängig von den Kombinationen Wirtsinsekt/Wirtspflanze, in denen sich die Parasiten entwickelt hatten. Die Ergebnisse weisen darauf hin, dass die Pflanze, auf der die Wirtsraupe frisst, wichtig ist für die Wahrscheinlichkeit eines Parasitenangriffs und für den Erfolg der Parasitierung.
    Notes: Summary Microplitis croceipes (Cresson), a parasitoid of Heliothis larvae, was reared on 9 host insect/plant combinations. In general, parasitoid survivorship was (1) higher in host larvae that were reared on cotton than in hosts reared on either bean or tomato, (2) higher in H. zea hosts than in H. virescens and (3) higher in a laboratory strain of H. zea than in a wild strain. A series of parasitoid oviposition preference tests showed that wasps laid more eggs in host larvae on cotton plants than on bean and more on bean than on tomato. However, the wasps did not distinquish between H. zea and H. virescens hosts. Wasp oviposition preferences were not conditioned by the host insect/plant combination in which the parasitoid developed. These results indicate that the plant on which host larvae feed is an important factor in determining both the likelihood of parasitoid attack and the probability of successful parasitism.
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  • 5
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    Entomologia experimentalis et applicata 34 (1983), S. 155-162 
    ISSN: 1570-7458
    Keywords: Hymenoptera ; Braconidae ; Lepidoptera ; Nymphalidae ; Apanteles bignellii ; Euphydryas aurinia ; Multivoltinism ; Synchronisation ; Weather
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung In einer Population von Euphydryas aurinia (Rottemburg) bei Oxford, England treten während einer Generation von E. aurinia drei Normalgenerationen von Apanteles bignellii Marshall auf. Jede Generation des Parasitoids kann charakterisiert werden durch das befallende Wirtsstadium und durch die aus einem Wirt schlüpfende Zahl Parasitoide. Parasitoide schlüpfen in Normalgenerationen aus dem 3., dem 4. und dem 6. Stadium des Wirts; in Ausnahmegenerationen schlüpfen sie aus dem 2. und 5. Stadium. Bis zu 70 Parasitoide können aus einer Altraupe (6. Stadium) schlüpfen und die Dauer dieses Stadiums kann bis auf 2 Wochen verlängert werden. Die Ausnahmegenerationen von A. bignellii aus Zweitlarven dürften aus Eiablagen in frühe Erstlarven stammen. Fünftlarven des Wirts, aus denen Parasitoide schlüpfen, sind ungewöhnlich klein und fressen nicht; sie dürften das Resultat sein eines Uebermasses von Apenteles-Eiern, die in frühe Viertlarven gelegt wurden. Die Synchronisation zwischen dem Parasitoiden und dem Wirt während der Zeit, da E. aurinia im Puppen-, Adult- oder Eistadium ist, wird aufrechterhalten durch ein verlängertes Coconstadium von Apanteles. Die Puppen des Parasitoiden entwickeln sich normal und die Adulten schlüpfen, bleiben aber bis 4 Wochen lang im Cocon, bevor sie sich eine Ausgangsöffnung machen. Das Wetter kann den Parasitierungsgrad der letzten Wirtsstadien beeinflussen. Wenn der Frühling kalt ist mit klarem Himmel, kann die Synchronisierung zwischen Parasitoiden, die aus Viertlarven des Wirts schlüpfen und potentiellen Fünft- und Sechtstlarven des Wirts schlecht werden. Die Entwicklung von Apanteles-Puppen wird durch die Umgebungstemperatur beeinflusst, während E. aurinia-Larven ihre Temperatur erhöhen, indem sie sich sonnen und deshalb rasch wachsen. Wenn die Parasitoiden unter solchen Bedingungen schlüpfen, sind die meisten potentiellen Wirte schon verpuppt und damit nicht mehr geeignet für die Parasitierung. Die Mechanismen der Synchronisation und der Wettereinfluss auf diese Vorgänge wird diskutiert.
    Notes: Abstract The gregarious endoparasite, Apanteles bignellii Marshall is specific to the nymphalid butterfly, Euphydryas aurinia (Rottemburg) in the British Isles. The synchronisation between host and parasitoid is described at a site near Oxford, England where both occur. Three regular generations of A. bignellii occur in one generation of the host in the studied population. Relevant features of the biology of A. bignellii and E. aurinia are described, including a method of distinguishing the number of Apanteles larval instars based upon shed cuticle remnants. Mechanisms for host-parasitoid synchronisation are outlined, especially a protracted parasitoid cocoon stage when the host is unavailable for attack during the chrysalis, adult and egg stages. Cool, but sunny weather conditions in spring can influence the degree of parasitisation experienced by final instar host caterpillars. The timing of adult A. bignellii emergence and subsequent attack on early instar hosts can lead to additional, partial, generations of parasitoids from second and fifth instar hosts.
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  • 6
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    Entomologia experimentalis et applicata 34 (1983), S. 208-211 
    ISSN: 1570-7458
    Keywords: Dieretiella rapae ; Encyrtidae ; Insecticide ; Integrated control ; Hymenoptera ; Pteromalidae ; searching behavior ; Syrphidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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  • 7
    ISSN: 1570-7458
    Keywords: Coleoptera ; Curculionidae ; Cylas formicarius elegantulus ; sweetpotato weevil ; sweet potato ; digestion ; enzyme distribution ; trypsin-inhibitors ; resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé L'activité endoprotéinase était limitée au liquide de la lumière des régions ventriculaires antérieure et postérieure de l'intestin moyen des larves deCylas formicarius elegantulus Summers. Les aminopeptidases ont été trouvées dans le liquide de la lumière (18%), mais étaient principalement associées avec les fractions insolubles des cellules du ventricule postérieur (82%). Suivant le substrat, l'activité carboxypeptidase était à peu près également répartie entre le liquide de la lumière et les fractions insolubles des cellules du ventricule postérieur. L'amylase, enzyme secrétée, a été observée à la fois dans la lumière antérieure et postérieure. Cinq bandes d'activité amylase ont été révélées sur zymogrammes d'amidon après électrophorèse d'intestins moyens entiers. Trois des bandes d'amylase pouvaient provenir de la plante. Il y avait au moins 2 α-glucosidases, une secrétée et une liée aux cellules du ventricule antérieur. L'activite β-glucosidase était liée aux cellules du ventricule antérieur, fandis que l'activité α-galactosidase était limitée au fluide de la lumière. Les activités β-galactosidase et cellulase ont été trouvées à la fois dans le liquide de la lumière et dans la fraction cellulaire insoluble. Ainsi, la digestion initiale des grosses protéines et des polymères de carbohydrates se produit dans le liquide de la lumière dans les régions ventriculaires antérieure et postérieure. Cependant, l'achèvement de la digestion des oligopeptides se produit principalement dans le ventricule postérieur, tandis que la fin de la digestion des oligosaccharides se produit dans le ventricule antérieur. Les concentrations en inhibiteurs de trypsine de 5 cultivars d'I. batatas L. diffèrent de 1 à 20. Les endoprotéinases deC. formicarius elegantulus ont été inhibées par des extraits de cultivars, mais les cultivars avec des concentrations relativement élevées d'inhibiteur avainent antérieurement montré être susceptibles d'être attaqués par le coléoptère dans des essais en champs.
    Notes: Abstract Endoproteinase activity was confined to luminal fluid from anterior and posterior ventricular regions of midguts of larvae of the sweetpotato weevil,Cylas formicarius elegantulus (Summers). Aminopeptidase was found in luminal fluid (18%) but was primarily associated with insoluble fractions from cells of the posterior ventriculus (82%). Depending on substrate, carboxypeptidase activity was about equally distributed between luminal fluid and insoluble fractions from posterior ventriculus cells. Amylase was found in luminal fluid in both the anterior and posterior ventriculus. Five bands of amylase activity were detected on starch zymograms following electrophoresis of whole midgut samples. Three of the amylase bands may be plant-derived. At least two α-glucosidases were present, one secreted and one bound to anterior ventriculus cells. β-Glucosidase activity was bound to anterior ventriculus cells whereas α-galactosidase activity was confined to luminal fluid. β-Galactosidase and cellulase activities were found in both luminal fluid and the insoluble cell fraction. Thus, initial digestion of large protein and carbohydrate polymers occurs in luminal fluid in both anterior and posterior ventriculus regions. However, terminal digestion of oligopeptides occurs primarily in the posterior ventriculus while terminal digestion of oligosaccharides occurs in the anterior ventriculus. A 20-fold difference in trypsin-inhibitor concentration was found among five sweetpotato cultivars. Endoproteinases from sweetpotato weevil larvae were inhibited by extracts from the cultivars but cultivars with relatively high concentrations of inhibitor were previously shown to be susceptible to weevil attack in field trials.
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  • 8
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    Entomologia experimentalis et applicata 35 (1984), S. 205-207 
    ISSN: 1570-7458
    Keywords: Hymenoptera ; Formicidae ; ants ; gland volumes ; lumen ; attine and myrmicine glands ; hydrocarbon reservoir
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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  • 9
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    Entomologia experimentalis et applicata 36 (1984), S. 31-35 
    ISSN: 1570-7458
    Keywords: Colpoclypeus florus ; Hymenoptera ; Eulophidae ; integrated pest management ; mass rearing ; biological control ; Adoxophyes orana ; Archips podana ; Archips rosana ; Clepsis spectrana ; Hedya nubiferana ; Pandemis heparana ; Ptycholoma lecheana ; Spilonota ocellana
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Colpoclypeus florus Walk., ectoparasite grégaire de Tortricidae, est capable de provoquer un taux de parasitisme élevé dans plusieurs types de cultures, y compris dans les vergers de pommiers. Dans les vergers aux Pay-Bas, ce parasite n'abonde qu'en été sur la première génération d'Adoxophyes orana. Les autres tortricides échappent à ses attaques, parce qu'elles ne peuvent être attaquées qu'au printemps. Le but de ces études était donc la mise au point d'une technique d'élevage de masse de C. florus, pour relâcher ce parasite en grand nombre dans les vergers au printemps. La technique décrite a permis de produire plusieurs milliers de parasites par semaine. Des lâchers de 16 000–28 000 parasites par ha dans un verger soumis à un programme de lutte intégrée, bien que faisant des victimes parmi toutes les espèces importantes de tortricides présentes, étaient loin d'occasionner le taux de parasitisme escompté. Le nombre de degrés-jours nécessaire au développement des différents stades de C. florus a été déterminé au laboratoire, et son cycle évolutif, inconnu aux Pays-Bas, puisque le parasite ne se trouve qu'en été, a été calculé et comparé aux observations faites en élevages en plein air. C. florus a de 4 à 5 générations par an, et la comparaison de sa phénologie avec celle des différentes tordeuses explique son absence hors de l'été. Au laboratoire, Archips podana était plus parasité, et Pandemis heparana moins parasité qu'A. orana. Le nombre moyen de descendants produits par femelle de C. florus et par chenille-hôte variait selon les hôtes, dépendant probablement de la taille des chenilles. Bien qu'A. orana n'ait pas produit le plus grand nombre de parasites par chenille, elle a été néanmoins préférée aux autres tortricides comme hôte pour les élevages.
    Notes: Abstract Colpoclypeus florus Walk. is a gregarious ectoparasite of tortricids. Details of biology and annual cycle in orchards in the Netherland are reported. It has four to five generations per year, but only females of the second generation, and some of the third, meet with abundant hosts (Adoxophyes orana F.v.R.) of the proper size. Therefore, the parasite is scarce to virtually absent from Duth orchards except in July and August. A technique for mass-rearing of C. florus is described, producing 6 000 wasps per week. 16 000–28 000 wasps/ha were released in spring, in order to control leafrollers. Although all important leafroller species were parasitized, the rate of parasitization was too low to be effective.
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  • 10
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    Entomologia experimentalis et applicata 34 (1983), S. 124-126 
    ISSN: 1570-7458
    Keywords: Negret ; Melanic mutant ; X-linked mutation ; Flour Beetle ; Tribolium confusum ; Coleoptera
    Source: Springer Online Journal Archives 1860-2000
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  • 11
    ISSN: 1570-7458
    Keywords: Storage pests ; Coleoptera ; Bostrichidae ; Prostephanus truncatus ; (±)-1-methylbutyl (E)-2-methyl-2-pentenoate ; Rhyzopertha dominica ; (±)-1-methyl-butyl (E)-2,4-dimethyl-2-pentenoate ; Dominicalure ; Aggregation pheromone ; Monitoring
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé Les réponses de Prostephanus truncatus Horn (Coleoptera: Bostrichidae) à deux constituants de la phéromone d'agrégation de Rhyzopertha dominica Fabricius (Coleoptera: Bostrichidae), le (±)-1-méthylbutyl (E)-2-méthyl-2-penténoate et le (±)-1-méthylbutyl (E)-2,4-diméthyl-2-pentéoate, connus comme “Dominicalure 1” et “Dominicalure 2”, ont été étudiées au laboratoire et au champ. Auslaboratoire ces composés ont été essayés individuellement et en mélange 1:2. Une membrane de caoutchouc imprégnée de phéromone a été utilisée comme émetteur lent, et une courbe de réponse en fonction de la dose a été établie. P. truncatus répondait plus vigoureusement à la Dominicalure 2 seule. Tous ces traitements ont été comparés dans un essai aux champs en Tanzanie, dans lesquels des pièges en carton ondulé contenant des diffuseurs de phéromone étaient placés dans des entrepôts de maïs dans les fermes. La Dominicalure 1 et le mélange de 1 et 2 étaient tous deux efficaces pour piéger R. dominica. La Dominicalure 2 a piégé plus de P. truncatus que les autres traitements et était à peu près aussi efficace que le contrôle visuel pour détecter la présence de cet insecte dans les stocks. On peut envisager que la Dominicalure 2 pourrait servir de base à un programme pour lutter contre P. truncatus en Afrique de l'Est.
    Notes: Abstract Laboratory and field studies have been made of the responses of Prostephanus truncatus (Horn) to two components of the aggregation pheromone of Rhyzopertha dominica (Fabricius) — (±)-1-methylbutyl (E)-2-methyl-2-pentenoate and (±)-1-methylbutyl (E)-2,4-dimethyl-2-pentenoate, known as “Dominicalure 1” and “Dominicalure 2” respectively. In the laboratory, these compounds were tested individually and as a 1:2 mixture; P. truncatus responded most strongly to Dominicalure 2 alone. All three treatments were compared in a field trial in Tanzania to monitor both R. dominica and P. truncatus in farm stores. Dominicalure 1 or a mixture of 1 and 2 were both highly effective for trapping R. dominica. In contrast, Dominicalure 2 trapped more P. truncatus than the other treatments and was about as effective as visual inspection at demonstrating the presence of the beetle in stores. Dominicalure 2 could form the basis of a monitoring programme for P. truncatus in East Africa.
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  • 12
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    Entomologia experimentalis et applicata 35 (1984), S. 43-47 
    ISSN: 1570-7458
    Keywords: Opius concolor ; Hymenoptera ; Braconidae ; sex ratio ; host size ; female age
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Résumé L'influence de la taille de l'hôteret de l'âge de la femelle sur le taux sexuel de la descendance a été étudiée sur le parasitoïde Opius concolor Szépl. Le taux sexuel de la descendance des femelles isolées est favorable aux femelles (t.s.=0,26) quand elles ont à leur disposition des hôtes de grande taille, tandis qu'avec des hôtes petits le taux sexuel est favorable aux mâles (t.s.=0.73). On n'a pas détecté de mortalité différentielle des sexes. Les pourcentage de mâles obtenu le premier jour de ponte des femelles sur les hôtes de grande taille est significativement différent de la moyenne, indépendamment de l'âge de la femelle à ce moment. Cependant, sur des hôtes petits, bien que restant favorable aux mâles dans l'ensemble, une plus grande proportion de femelles à partir des premiers jours de ponte a pu être observée.
    Notes: Abstract The sex ratio of the progeny of single females parasitizing large hosts favoured the females (sex ratio=0.26); but on small hosts favoured the males (0.73). No differences in mortality of the sexes were detected. The sex ratio was independent of female age when large hosts were used. The percentage of males observed in the progeny of the first day of female oviposition was significantly greater than the mean, irrespective of the age at which female oviposition began. When females were exposed to small hosts, a greater percentage of females was observed in the progeny from the last days of oviposition.
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  • 13
    ISSN: 1432-1432
    Keywords: Yeast ; E. coli ; tRNA ; rRNA ; Sequence homologies ; Evolution ; Origins ; Coding mechanism
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Many tRNAs ofE. coli and yeast contain stretches whose base sequences are similar to those found in their respective rRNAs. The matches are too frequent and extensive to be attributed to coincidence. They are distributed without discernible pattern along and among the RNAs and between the two species. They occur in loops as well as in stems, among both conserved and non-conserved regions. Their distributions suggest that they reflect common ancestral origins rather than common functions, and that they represent true homologies.
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  • 14
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    Nutrient cycling in agroecosystems 5 (1984), S. 371-382 
    ISSN: 1573-0867
    Keywords: nitrogen ; phosphorus ; dry areas ; apparent recovery fraction ; fertilizer management
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Agronomic efficiency (AE) is defined as the increase in economic yield of a crop per unit fertilizer applied. Components of AE are physiological efficiency and apparent recovery fraction. The latter can be further separated into uptake efficiency and availability index. Ways to increase the nitrogen fertilizer use efficiency in rainfed agriculture through fertilizer management are discussed.
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    Development genes and evolution 192 (1983), S. 42-44 
    ISSN: 1432-041X
    Keywords: Chick embryo ; Gastrulation ; Adenylate cyclase ; cAMP phosphodiesterase ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The ultrastructural localization of adenylate cyclase (E.C. 4.6.1.1.) and cAMP phosphodiesterase (PDE) (E.C. 3.1.4.17.) in the ectoderm of the developmental stage 4 chick embryo was studied. Adenylate cyclase was localized in the lateral surfaces of the ectodermal cells. In the primitive streak cells the enzymatic activity was observed on all the lateral surfaces, whereas in the periphery of the blastoderm the reaction product was localized in the apical parts of the lateral plasma membranes only. cAMP PDE localized in the apical cytoplasm of the ectodermal cells, with highest activity in the globular projections.
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    Development genes and evolution 192 (1983), S. 171-178 
    ISSN: 1432-041X
    Keywords: Differentiation ; Digestive tract ; Endoderm ; Organ culture ; Ultrastructure
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    Topics: Biology
    Notes: Summary The self-differentiation potency of the endoderm of the chick embryo was investigated mainly by transmission electron microscopy. Endodermal fragments isolated from 4- to 6-day stomach or small intestine were cultured in the absence of mesenchyme and were able to differentiate in vitro into organ-specific epithelia. Endodermal fragments isolated from the stomach region differentiated into a pseudo-stratified epithelium with periodic acid Schiff-positive mucous granules in the apical cytoplasm, while those from the small intestinal region differentiated into a simple columnar epithelium with a striated border which was positive in alkaline phosphatase activity. These features are comparable with those of the mucous secretory epithelium of the normal embryonic stomach and the absorptive epithelium of normal embryonic small intestine, respectively. Next, the self-differentiation potencies were investigated of the upper and lower layers of the blastoderms, at stages 1–5 of Hamburger and Hamilton (H. and H.). Both stomach-type and small-intestine-type epithelia developed only when fragments of the lower layer isolated from the blastoderms older than stage 3 of H. and H. were cultured, suggesting that cells possessing the potency to differentiate into the stomach- and small-intestine-type epithelia exist in the definitive endoderm at the beginning of its formation.
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    Calcified tissue international 36 (1984), S. 550-555 
    ISSN: 1432-0827
    Keywords: Enamel crystals ; Length ; Shape ; Apatite ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary An original method for fractionating and preparing isolated crystals of homogeneous size was developed. It was demonstrated that enamel apatite crystals are at least 100 µm long. The flexibility of the very long crystallites was demonstrated. Crystal curvatures, accounting for the irregular course of the prisms through the enamel thickness, were visualized and measured. It was shown that in the deep forming enamel layer, lateral branches may grow out of the crystals and crystal fusing often occurs, inducing the crystallites to assume pyramidal shapes with their wide bases pointing toward the dentino-enamel junction and one or two tops toward Tomes' processes. During the maturation process, the two tops of the still immature crystals also fuse so that the mature crystals acquire a rodlike aspect, with parallel faces and steplike graduations along thec axis, allowing a close contact between the crystals. These results support the hypothesis that the crystallites would be continuous from the dentino-enamel junction to the surface.
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    Current genetics 8 (1984), S. 49-55 
    ISSN: 1432-0983
    Keywords: Protoplast fusion ; Yeast ; Yarrowia lipolytica ; Kluyveromyces lactis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Prototrophic hybrids have been obtained by the fusion of auxotrophic haploid strains of the two yeasts Yarrowia (Saccharomycopsis) lipolytica and Kluyveromyces lactis. The hybrid fusants had a colonial morphology intermediate between that of the two parent strains, were uninucleate, and contained an approximately diploid amount of DNA per cell. The growth rates of all the fusants on a minimal glucose medium were slower than those of the two parents. Two of the fusants studied could utilise a novel range of carbon sources. All of these data suggested that the hybrids contained a diploid nucleus formed by the fusion of the two haploid parental nuclei. However, analytical CsCl density gradient centrifugation demonstrated that the nuclear DNA of the fusants was derived almost entirely from the Y. lipolytica parent. Moreover, an examination of the protein constitution of the fusants by two-dimensional gel electrophoresis showed that their protein patterns were indistinguishable from that of Y. lipolytica. Two possible mechanisms for the formation of a diploid nucleus containing DNA derived almost entirely from one of the haploid parents are discussed.
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    Current genetics 7 (1983), S. 473-480 
    ISSN: 1432-0983
    Keywords: ars sequences ; Yeast ; Chlamydomonas
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    Topics: Biology
    Notes: Summary A hybrid plasmid (pLG4) containing pBR325 and the yeast arg4 gene was constructed then used to isolate DNA fragments of Chlamydomonas able to promote high frequency transformation of yeast. Three plasmids containing EcoRI restriction fragments of chloroplast DNA and two plasmids containing Aval fragments of nuclear DNA were shown to support autonomous replication of plasmids in yeast. The three EcoRI fragments correspond to restriction fragments R4, R5 and R11 of native chloroplast DNA. These fragments are clustered in the physical map of chloroplast DNA constructed by Rochaix (1978). All isolated plasmids were shown to transform yeast at high frequency but the yeast transformants were quite unstable mitotically. Potential cloning sites are still available in the new plasmids which could be used as vectors in yeast and possibly in Chlamydomonas itself.
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  • 20
    ISSN: 1432-0983
    Keywords: Yeast ; Mitochondrial DNA ; Antibiotic resistance mutations ; Suppressor
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    Topics: Biology
    Notes: Summary Strains that are genotypically sensitive to chloramphenicol and also contain one of the nuclear suppressors of mitochondrial chloramphenicol resistance (Waxman et al. 1979) were constructed. A manganese mutagenesis on such a strain produced chloramphenicol resistant mutants, most of which resulted from mutations in nuclear genes. These mutants may be either dominant or recessive, and they probably do not code for membrane proteins. The few mitochondrial mutants fall into several classes, but all result from mutations in the 21S rRNA gene. The suppressor allele effectively prevents the appearance of the most common group of mitochondrial mutants (those that map at cap1), and thereby enhances the selection of novel mutants in the region.
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  • 21
    ISSN: 1432-0983
    Keywords: Yeast ; Mitochondria ; Intragenic recombination ; Mutant polypeptides
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    Topics: Biology
    Notes: Summary Genetic and biochemical studies were performed with mutants allocated to the mitochondrial oxi2 gene. Recombinational analysis of 19 oxi2 mutants was performed using α and a mutant strains derived from the same genetic background. The frequencies of wild-type recombinants in oxi2 − × oxi2 − crosses varied from 0.002 to 17%. The map of oxi2 mutations constructed on the basis of these frequencies shows many internal inconsistencies. In the course of rho − deletion mapping five classes of oxi2 mutations were distinguished. The results of deletion analysis are in agreement with those of recombinational mapping. The analysis of mitochondrial translation products by SDS-polyacrylamide electrophoresis of 20 oxi2 mutants shows that 17 of them are connected with conspicuous changes of 22 kd polypeptide band corresponding to subunit III of cytochrome oxidase. At least four of them carried instead of subunit III clearly visible significantly shorter polypeptides (12.8 to 20.1 kd). These were, most likely, shorter fragments of subunit III resulting from chain termination mutations. Colinearity was observed between the lenght of new polypeptides and the positions of the respective mutations on the recombinational map. These data confirm hat oxi2 encodes subunit III of cytochrome oxidase and suggest that translation of the oxi2 gene is in the direction from V303 to V273.
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    Current genetics 7 (1983), S. 285-288 
    ISSN: 1432-0983
    Keywords: Arginyl-tRNA-Protein transferase ; Yeast ; Post-translational modification
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    Topics: Biology
    Notes: Summary A mutant of Saccharomyces cerevisiae deficient in arginyl-tRNA-protein transferase has been isolated. The responsible mutation designated ate1, was localized near the centromere of chromosome VII. It probably involves the structural gene for the transferase since residual enzyme activity in the mutant is temperature-sensitive.
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    Current genetics 7 (1983), S. 489-492 
    ISSN: 1432-0983
    Keywords: Mitochondrial genes ; Yeast ; Vegetative segregation
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    Topics: Biology
    Notes: Abstract Genes in mitochondria and chloroplasts segregate rapidly during vegetative reproduction. Models to explain this vegetative segregation invoke either random segregation of organelle DNA molecules, or nonrandom segregation with random recombination events. All such models are basically stochastic. To look at vegetative segregation we took heteroplasmic (HET) cells containing mitochondrial mutations at the cap1, eryl and olil loci from several crosses. HETs were repeatedly selected and subcloned. Even after three to five successive subclonings (approximately 60–100 generations) some cells remained heteroplasmic. This confirms and extends previous observations of persistent HETs by Rank and Bech-Hansen (1972) and Forster and Kleese (1975), and by Bolen et al. (1980) for chloroplast genes in Chlamydomonas.
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    Current genetics 8 (1984), S. 29-32 
    ISSN: 1432-0983
    Keywords: Antisuppression ; Suppression ; tRNA ; Yeast
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    Topics: Biology
    Notes: Summary The effect of a previously isolated antisuppressor mutation from bakers' yeast, that reduced the efficiency of the tyrosine-inserting ochre suppressor, SUP7-o, on other tyrosine-inserting ochre suppressors has been determined. As expected, the antisuppressor mutation, mod5-1, restricted the capacity of all eight tyrosine-inserting ochre suppressors to suppress nonsense mutations. Based on the suppression of five ochre alleles in the presence of mod5, the eight class I suppressors can be grouped into three subclasses. The most efficient subclass had only one member, SUP4-o. Members of the second group included SUP2-o, SUP3-o, SUP7-o, and SUP8-o. The third and least efficient subclass included SUP5-o, SUP6-o, and SUP1 1-o. These differences in efficiencies are a function of the relative expression of the eight genes encoding tRNATYR.
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    Current genetics 8 (1984), S. 85-92 
    ISSN: 1432-0983
    Keywords: Chromosome map ; Yeast ; Schizosaccharomyces pombe ; Gene conversion
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    Topics: Biology
    Notes: Summary The genetic map of the nuclear genome of the fission yeast Schizosaccharomyces pombe has been extended by mitotic and meiotic mapping data. A total of 158 markers are now assigned to the three linkage groups known in this organism, and 118 of them have been located on the corresponding chromosome map. Chromosome II and III each consist of one linkage group. There is some indication that the two large fragments which define chromosome I are meiotically linked, but the linkage observed is significant at the P = 0.05 level only. The length of the map is at least 1,700 map units, corresponding to an average of about 8 kilobases per map unit. The latter figure is comparable to the one obtained for intragenic recombination in the sup3 gene (Hofer et al. 1979). The basic frequency of gene conversion as measured for 21 genes varies according to a distribution of Poisson (with a modal value of 0.6% conversion per meiosis and per gene), in sharp contrast with Saccharomyces cerevisiae (Fogel et al. 1980) and Ascobolus immersus (Nicolas 1979). This may reflect the rarity of gene or region-specific rec alleles in S. pombe and may be related to the homothallism of this organism.
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    Current genetics 8 (1984), S. 353-358 
    ISSN: 1432-0983
    Keywords: Hygromycin B ; Yeast ; Plasmids
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    Topics: Biology
    Notes: Summary Saccharomyces cerevisiae is normally sensitive to the drug hygromycin B; a hygromycin B concentration of 200 µg/ml in agar plates is sufficient to completely inhibit growth. We constructed yeast-E. coli bifunctional plasmids which confer hygromycin B resistance to Saccharomyces cerevisiae. Promoters and amino terminal coding regions of a heat shock gene, a heat shock cognate gene, and the phosphoglycerate kinase gene from yeast were fused to a bacterial hygromycin B resistance gene. In all three cases, yeast cells containing plasmids with the hybrid hygromycin B resistance gene were resistant to high levels of the drug. Yeast cells containing these plasmids can also be directly selected after transformation by using hygromycin B. The intact bacterial hygromycin B resistance gene and the kanamycin resistance gene from Tn903 were also tested in yeast for their ability to confer resistance to hygromycin B and G418. The intact bacterial genes were not effective in conferring drug resistance to yeast cells.
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    Current genetics 7 (1983), S. 85-92 
    ISSN: 1432-0983
    Keywords: Yeast ; RAD52 ; Cloning ; S1 and BAL31 Deletions
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    Topics: Biology
    Notes: Summary The RAD52 gene of Saccharomyces cerevisiae has previously been shown to be involved in both recombination and DNA repair. Here we report on the cloning of this gene. A plasmid containing a 5.9 kb yeast DNA fragment inserted into the BamH1 site of the YEp13 vector has been isolated and shown to complement the X-ray sensitive phenotype of the rad52-1 mutation. The rad52-1 cells containing the plasmid form larger colonies than similar cells having lost the plasmid. This plasmid has been shown not to complement either the U.V. sensitivity or the recombination defect of the E. coli recA mutation. From the insert various fragments have been subcloned into the YRp7 and YIp5 vectors. Integration events of two of the subclones have been genetically mapped to the chromosomal location of RAD52, indicating that the structural gene has been cloned. A 1.97 kb BamH1 fragment subcloned into YRp7 in one orientation complements the rad52-1 mutation, while the same fragment in the opposite orientation fails to complement. Various other subclones indicate that a BglII site, within the BamH1 fragment, is in the RAD52 gene. This BglII site has been deleted by Sl-nuclease digestion and the resulting deletion inactivates the RAD52 gene. BAL31 deletions from one end of a 1.9 kb Sal1-BamH1 fragment have been isolated; up to 0.9 kb can be deleted without loss of RAD52 activity, indicating that the RAD52 gene is approximately 1 kb or less in length.
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    Current genetics 8 (1984), S. 69-76 
    ISSN: 1432-0983
    Keywords: Yeast ; Ethidium bromide ; Meiosis
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    Topics: Biology
    Notes: Summary Ethidium bromide was found to inhibit nuclear and mitochondrial DNA synthesis during meiosis which resulted in the inhibition of meiotic gene conversion and sporulation and was also lethal. Protection from the effects of ethidium bromide on meiotic gene conversion and survival was found to coincide with DNA synthesis, but it is possible that protection from sporulation inhibition occurs only later in meiosis.
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    Current genetics 8 (1984), S. 333-340 
    ISSN: 1432-0983
    Keywords: polA+ ; DNA polymerase I ; Cloning ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The E. coli polA + gene has been subcloned from a specialised λ transducing phage onto a low copy number plasmid. Plasmid-encoded DNA polymerase I was synthesised at 2 to 3 times the wild-type E. coli level, and was biochemically indistinguishable from chromosomally-encoded protein. It was able to counteract the radio sensitivity of polA1, polAex1, polAex2 and polA12 mutants, but no complementation of polA107 mutants occurred, even though the plasmid polA+ gene was expressed. S. cerevisiae ars-1 or 2 μ replicative sequences were introduced into the polA+ plasmid. Transformation of yeast with these constructs increased total DNA polymerase levels 2–20 times, depending upon assay conditions. The additional activity was discriminated from yeast DNA polymerases by its ability to use low concentrations of substrate, by its resistance to chemical inhibition, and by co-electrophoresis with pure DNA polymerase I and its proteolytic fragments. The polA+ gene was expressed in yeast without the aid of yeast promotor sequences. However, deletion of cloned DNA more than 99 base pairs in front of the structural gene prevented expression in yeast but not in E. coli, indicating that the two organisms use different sequences for expression of the plasmid polA+ gene.
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    Current genetics 8 (1984), S. 471-475 
    ISSN: 1432-0983
    Keywords: β-Glucanase ; Expression ; Heterologous DNA ; Yeast
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    Topics: Biology
    Notes: Summary A cloned endo-1,3-1,4-β-glucanase gene from the Gram-positive bacterium B. subtilis has been located by deletion analysis on a 1.4 kb PvuI-ClaI DNA fragment. This gene has been sub-cloned in the yeast LEU2 vector pJDB207 to produce a hybrid plasmid designated pEHB9. pEHB9 has been transformed to S. cerevisiae and shown to direct the synthesis of an endo-1,3-1,4-β-glucanase in yeast. The β-glucanase activity was low and could only be detected in crude cell extracts of yeast harbouring pEHB9.
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    Current genetics 7 (1983), S. 69-72 
    ISSN: 1432-0983
    Keywords: Yeast ; Protoplast ; Cybrid ; Plasmid
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    Topics: Biology
    Notes: Summary Data presented here demonstrate that fusion of protoplasts of two different haploid strains of Saccharomyces cerevisiae having the same mating type leads to the formation of “fusants” and “cytoplasmic hybrids”. The nuclear and cytoplasmic genome of a “fusant” combine those of the parent haploid strains. The “cytoplasmic hybrid” possesses the haploid genome of one parent and the combined cytoplasmic genomes of both. In mouse cells lines such products have been termed “cybrids” and this term has therefore been adopted here (Bunn and Wallace 1974).
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    Current genetics 7 (1983), S. 93-100 
    ISSN: 1432-0983
    Keywords: Yeast ; RAD genes ; Cloning
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    Notes: Summary Plasmids that complement the yeast mutations rad50-1, rad51-1, rad54-3 and rad55-3 were obtained by transforming strains that carried a leu2 marker and the particular rad mutation, with YEp13 plasmids containing near random yeast DNA inserts. Integration of these plasmids or of fragments of these plasmids was accomplished. Genetic studies using the integrants established the presence of the genes RAD51, RAD54 and RAD55 in the respective plasmids. However, a BamHI subclone of the rad50-1 complementing plasmid failed to integrate at the RAD50 locus, indicating that no homology exists between this fragment and the RAD50 gene. A BamHI fragment from the RAD54 plasmid was shown to be internal to the RAD54 gene: its integration within a wild type copy of RAD54 causes the cell to become Rad−; its excision is X-ray inducible and restores the Rad+ phenotype. Since cells bearing a disrupted copy of RAD54 are able to survive, we conclude that this gene is not essential.
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  • 33
    ISSN: 1432-0983
    Keywords: Yeast ; Mutant ; Uncoupler ; Resistance ; Permeability
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    Notes: Summary One mutant resistant to carbonylcyanide m-chlorophenylhydrazone (CCCP), an uncoupler of oxidative phosphorylation, was isolated in Saccharomyces cerevisiae. Genetic analysis showed that a single nuclear gene is responsible for increased resistance; this gene was dominant. The mutant showed cross-resistance or collateral sensitivity to several chemically-unrelated inhibitors (cycloheximide, dinitrophenol, tributhyltin chloride, chloramphenicol). The resistance of the mutant is related to a decreased uptake of CCCP which is not expressed in glucose-starved cells. It was shown that glucose induced a CCCP efflux which was more efficient in the mutant than in the wild-type cells. This effect was correlated to a greater acidification of the internal pH by glucose addition in the mutant cells. It was proposed that resistance was not due to a change of permeability of the plasmic membrane itself but to the change of internal pH which determines the extent of accumulation of weak acids or bases.
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    Current genetics 8 (1984), S. 567-573 
    ISSN: 1432-0983
    Keywords: Non-Mendelian ; Yeast ; Suppressor
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    Notes: Summary Omnipotent suppressors cause translational ambiguity and have been associated with poor growth and inviability. We now report that a non-Mendelian element, [eta+], causes this inviability. In [eta−] strains the suppressors are not inviable. The [eta+] genetic element segregates to about 70% of the meiotic progeny, although almost all of the spores probably have the [eta+] phenotype for the first few divisions. Growth on 5 mM guanidine hydrochloride efficiently causes [eta+] strains to become [eta−]. The [eta+] factor has many similarities with the previously described [psi+] factor (Cox 1965, 1971). However, [eta+] and [psi+] differ in their patterns of inheritance, and by the fact that [psi+] affects ochre specific and not omnipotent suppressors, while the converse is true of [eta+].
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  • 35
    ISSN: 1432-0983
    Keywords: Canavanine ; Yeast ; Plasmids
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    Notes: Summary We have found that the application of the amino acid analog canavanine to a culture of yeast cells transformed with chimeric plasmids based on the yeast 2-µm DNA plasmid increases the percentage of cells which have lost the transforming plasmid. This effect is found whether the plasmid carries the CAN1 sensitive allele and the yeast strain carries a can1 mutation confering resistance, or the plasmid contains no CAN1 allele and the yeast strain carries the wild-type CAN1 sensitive allele. Canavanine exerts this effect on yeast strains transformed with chimeric plasmids containing either a portion or the entire 2-µm DNA plasmid, yet canavanine does not appear to effect the maintenance of the native 2-µm DNA plasmid complement within the cell. The effect of canavanine on strains transformed with chimeric plasmids is the same whether or not the yeast strain also contains native 2-µm plasmid DNA. Neither the amino acid analog ethionine, the protein synthesis inhibitor cycloheximide, nor the DNA replication inhibitor hydroxyurea exhibit this effect. Some of the experimental results suggest that canavanine may be a curing agent rather than an agent which selects for spontaneous plasmid loss.
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  • 36
    ISSN: 1432-0983
    Keywords: Yeast ; Polyamines ; Termination ; In vitro Translation
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    Notes: Summary The effects of polyamines (spermidine and putrescine) on yeast suppressor tRNA-mediated readthrough of amber and UGA termination codons, in a homologous cell-free system, was examined. The efficiency of readthrough in a [psi+] lysate, mediated by exogenous suppressor tRNA, was significantly increased by polyamines as was the efficiency of endogenous UGA readthrough. The addition of polyamines, in the absence of exogenous suppressor tRNA, did not induce amber or ochre readthrough, nor could polyamines restore efficient termination readthrough in [psi−] lysates. It is concluded that polyamines interact with tRNA to increase the strength and specificity of the codon: anticodon interaction.
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    Current genetics 8 (1984), S. 265-270 
    ISSN: 1432-0983
    Keywords: DHF Reductase ; Cloning ; Trimethoprim ; Yeast
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    Topics: Biology
    Notes: Summary The dihydrofolate reductase gene of Saccharomyces cerevisiae has been isolated by selection of trimethoprim resistant Escherichia coli transformed with a gene bank of yeast DNA in plasmid pBR322. From 9.2 kilobase pair BamHI DNA fragment this gene has been localized to a 1.76 kb fragment, the restriction map of which appears different from those reported for the E. coli and the mouse dihydrofolate reductase genes. The enzyme encoded by the chimeric plasmid was established as yeast dihydrofolate reductase by its sensitivity to antifolates in vivo through growth studies and in vitro by enzyme assay. Since, the expression of this gene occurs independent of its orientation within the chimeric plasmid, the 1.76 kb fragment may contain functional regulatory sequences in addition to the structural sequences for yeast dihydrofolate reductase.
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  • 38
    ISSN: 1432-0983
    Keywords: Yeast ; Ribosomal protein gene ; Sequence analysis ; Northern blot
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    Notes: Summary Sequence analysis revealed that a gene coding for yeast ribosomal protein L34 comprises an amino acid coding region of 339 nucleotides which is interrupted by an intron after the 19th codon. Like for other yeast ribosomal protein genes analyzed thus far a strong codon bias was observed. The flanking and intervening sequences of this gene encoding L34 show several elements that are conserved in a number of split ribosomal protein genes in yeast. Northern blot analysis using an intron-specific probe demonstrated that the sequenced gene copy coding for L34 is transcribed in vivo.
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    Current genetics 7 (1983), S. 79-84 
    ISSN: 1432-0983
    Keywords: ars sequences ; Ustilago ; Yeast
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    Notes: Summary U. maydis chromosomal DNA sequences which promote the autonomous replication of plasmid YIp5 in S. cerevisiae YNN27 have been isolated and three of them characterised in some detail. Their properties are idential to yeast ars sequences in that plasmids containing them are maintained extrachromosomally as circular double-stranded DNA molecules, are mitotically unstable in yeast transformants and transform yeast at high frequencies. There is no sequence homology between the three U. maydis sequences and they are not reiterated in the U. maydis genome.
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  • 40
    ISSN: 1432-0983
    Keywords: Yeast ; Recombination ; Conversion ; Restoration
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    Notes: Summary Heteroduplex repair models of recombination predict that restoration of the parental genotype from heteroduplex will have the same frequency as conversion to the genotype of the homologue. We have reported previously (Savage and Hastings 1981) that the proportion of intragenic reciprocal events in the his1 locus of yeast is too low for this expectation. In this study, two classes of recombination event involving longer lengths of his1 are compared in a series of crosses using a constant right-hand marker. This comparison gives a value for conversion: restoration for the left-hand markers which is independant of the method used before. The values obtained by the two methods are significantly correlated, confirming that there is a disparity in the ratio of conversion to restoration, and that this disparity is seen as a deficiency of restoration for five alleles of his1.
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    Current genetics 8 (1984), S. 429-437 
    ISSN: 1432-0983
    Keywords: Yeast ; Heat-shock response ; Mitochondrial inhibition ; 2D electrophoresis
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    Topics: Biology
    Notes: Summary Conventional and two dimensional (2D) electrophoresis on ultrathin horizontal slab gels shows that heat shock proteins are synthesized and heat stroke proteins are curtailed after the transfer of Saccharomyces cerevisiae strain Z270 from 23 °C to 37 °C. Upon addition of the mitochondrial translation inhibitor erythromycin to cell cultures which incorporated labelled methionine at 23 °C, and after the transfer to 37 °C, we have shown that: a) in extracts of cells labelled at 23 °C, translational products sensitive to erythromycin could be observed on 2D gels; the synthesis of some of these proteins was enhanced, whereas the synthesis of some others declined when the labelling was carried out 20 min after the transfer to 37 °C; b) there are heat shock proteins whose induction at 37 °C was prevented by erythromycin; c) labelling of a number of proteins became weaker at 37 °C, but not at 23 °C, when this was done in the presence of erythromycin; d) two proteins were detectable only in samples labelled at 37 °C in the presence of erythromycin.
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  • 42
    ISSN: 1432-0983
    Keywords: Yeast ; Mitochondrial ochre mutations ; Specificity of suppressors ; Mitoribosomal misreading ; Intron-encoded maturases
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We describe studies on the action spectra of the mitochondrial suppressor mim3-1 and the three alleles of nuclear suppressor nam3. Their specificity of action was tested on 516 mit − mutations located in different mitochondrial genes. The degree of suppression was quantified by the extent of cytochrome oxidase and cytochrome b synthesis. We show that the four suppressors are allele-specific gene-nonspecific informational suppressors. They would act by changing the structure of the small mitoribosomal subunit which would decrease fidelity of translation enabling misreading of some but not all ochre codons. The implications of the results on the role of intron encoded maturases are discussed.
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  • 43
    ISSN: 1432-0983
    Keywords: Yeast ; Mitochondrial ochre mutations ; Nuclear informational suppressors ; Mitochondrial informational suppressors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A systematic search for suppressors of mutations which cause a deficiency in the splicing of mitochondrial RNA has been undertaken. These splicing mutations were localized in the mRNA-maturase coding sequence of the second intron of the cob-boxgene, i.e. in the box3locus. A total of 953 revertants (mostly spontaneous in origin) were isolated and their genetic nature (nuclear vs. mitochondrial) and phenotype characterized. Most revertants were mitochondrially determined and displayed a wild-type phenotype. A mitochondrial suppressor unlinked with the box3 −target mutation was uncovered among the revertants displaying a pseudo-wild phenotype: out of 26 revertants analyzed, derived from 7 different box3− mutants only one such suppressor mutation mim3-1 was found. It was localized by rho− deletion mapping in the region between the oxi2 and oxi3 gene, within (or in the vicinity) the gene specifying the 15S ribosomal RNA. Nuclear suppressors were isolated from seven different box3 −mutants. All were recessive and had a pseudo-wild phenotype. Three such suppressors nam3-1, nam3-2 and nam3-3 were investigated more extensively. Tetrad analysis has shown that they are alleles of the same nuclear locus NAM3 and mitotic analysis has shown that they do not segregate mitotically.
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  • 44
    ISSN: 1432-072X
    Keywords: Yeast ; Phosphate uptake ; Phosphate-binding protein ; Anti-phosphate-binding protein antibodies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The purification of a phosphate-binding protein (PiBP2) by immunoadsorption is described. The entire anti phosphate-binding protein 2 antibodies as well as the Fab fragments obtained from these antibodies inhibit Pi uptake by whole cells. The inhibition is a mixed type of inhibition (V m and K m are affected). These results should be regarded as a possible involvement of phosphate-binding protein 2 in Pi uptake. The binding of 125I-labelled fragments prepared from anti phosphate-binding protein 2 antibodies to whole cells, to shocked cells and to protoplasts has been investigated. The results confirm the release of phosphate-binding protein by osmotic shock and during protoplast formation. From these findings, a cell-wall localisation, near the cell surface of the phosphate-binding protein should be proposed.
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  • 45
    ISSN: 1432-072X
    Keywords: Methanogenium tatii ; Ultrastructure ; Physiology ; Glycoproteins ; DNA-DNA Homology ; Taxonomy ; Archaebacteria
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    Topics: Biology
    Notes: Abstract A new coccoid methanogen, Methanogenium tatii, was isolated and characterized. The mesophilic isolate can grow on and produce methane from H2:CO2 and formate. For growth acetate is strictly required. The cell shape, the G+C content of 54 mol% and DNA-DNA homology data suggest it to be a Methanogenium species.
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  • 46
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    Archives of microbiology 137 (1984), S. 357-361 
    ISSN: 1432-072X
    Keywords: Yeast ; Saccharomyces cerevisiae ; Killer toxin ; Extracellular glycoprotein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A total of 13 killer toxin producing strains belonging to the genera Saccharomyces, Candida and Pichia were tested against each other and against a sensitive yeast strain. Based on the activity of the toxins 4 different toxins of Saccharomyces cerevisiae, 2 different toxins of Pichia and one toxin of Candida were recognized. The culture filtrate of Pichia and Candida showed a much smaller activity than the strains of Saccharomyces. Extracellular killer toxins of 3 types of Saccharomyces were concentrated and partially purified. The pH optimum and the isoelectric point were determined. The killer toxins of S. cerevisiae strain NCYC 738, strain 399 and strain 28 were glycoproteins and had a molecular weight of Mr=16,000. The amino acid composition of the toxin type K2 of S. cerevisiae strain 399 was determined and compared with the composition of two other toxins.
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  • 47
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    Archives of microbiology 138 (1984), S. 183-186 
    ISSN: 1432-072X
    Keywords: Yeast ; Nitrogen assimilation ; Nitrate reductase ; Nitrite reductase ; Candida utilis ; Food yeast ; Nitrate reduction ; Nitrogenous oxide
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Assimilation of nitrate and various other inorganic nitrogen compounds by different yeasts was investigated. Nitrate, nitrite, hydroxylamine, hydrazine, ammonium sulphate, urea and L-asparagine were tested as sole sources of nitrogen for the growth of Candida albicans, C. pelliculosa, Debaryomyces hansenii, Saccharomyces cerevisiae, C. tropicalis, and C. utilis. Ammonium sulphate and L-asparagine supported the growth of all the yeasts tested except D. hansenii while hydroxylamine and hydrazine failed to support the growth of any. Nitrate and nitrite were assimilated only by C. utilis. Nitrate utilization by C. utilis was also accompanied by the enzymatic activities of NAD(P)H: nitrate oxidoreductase (EC 1.6.6.2) and NAD(P)H: nitrite oxidoreductase (EC 1.6.6.4), but not reduced methyl viologen-or FAD-nitrate oxidoreductases (EC 1.7.99.4). It is demonstrated here that nitrate and nitrite reductase activities are responsible for the ability of C. utilis to assimilate primary nitrogen.
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  • 48
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    Archives of microbiology 138 (1984), S. 229-232 
    ISSN: 1432-072X
    Keywords: Actinomycetes ; Streptomyces torulosus ; Morphology ; Ultrastructure ; Verrucate spores ; Knobby ornamentation ; Sheath
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The type strain of Streptomyces torulosus Lyons and Pridham (1971) was studied by scanning- and transmission electron microscope. Spore chains were formed in spirals by aerial mycelium. The spores were connected by nozzles in which small channels could be observed. The knobby ornamentations of the spores arised on a thin fibrous sheath, enveloping the spore chains. These irregular blunt projections, called knobs, had varying diameters of 100 to 250 nm. The base of the knob, consisting of globose to flattened electron dense material, was sitting directly on the sheath. It was covered by several small vesicles of the same material. Each hollow vesicle beared a thin bowlshaped shell of electron transparent material. In general, the cupular bowls and their supporting vesicles became easily depressed on their base, but not detached from the surface of the spores. This type of knobby spore ornamentation was suggested to be designated as a verrucate spore type.
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  • 49
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    Archives of microbiology 137 (1984), S. 104-108 
    ISSN: 1432-072X
    Keywords: Chloroquine ; Yeast ; Proteolysis ; ATP hydrolysis ; Glucose consumption ; Ethanol formation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In yeast cells, degradation of cellular proteins was inhibited by addition of chloroquine to the medium as shown by a decrease of the release of trichloroacetic acid-soluble radioactivity from prelabelled cell protein. Penetration of chloroquine into the cells was strongly enhanced with increasing pH value of the medium. The concentration in the cells reached 5–14 times that in the medium of pH 8.0. Fluorescence microscopy showed that chloroquine was concentrated in the vacuoles of the cells. Chloroquine, at concentrations attained in the cells, inhibited the activities of vacuolar proteinases in vitro. Furthermore, chloroquine caused a rapid and drastic decrease of the ATP content of the cells and prevented the fermentation of glucose and formation of ethanol under aerobic conditions.
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  • 50
    ISSN: 1432-072X
    Keywords: Agglutination substance ; Cell-cell recognition ; Glycoprotein ; Mating ; Saccharomyces cerevisiae ; Sexual agglutinability ; Yeast
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    Topics: Biology
    Notes: Abstract An a-mating-type-specific substance responsible for sexual agglutination was purified to 397-times in specific activity (units/mg protein) from the cytoplasm of a-mating type cells. The purified substance gave a single band stained with PAS reagent but not with both Coomassie brilliant blue and silver staining reagent by polyacrylamide gel electrophoresis in the presence of 8 M urea. However, incorporation of [35S]methionine and Lowry reaction clearly indicate that the substance is a glycoprotein. The substance specifically masked sexual agglutinability of cells of the opposite mating type α, indicating univalent action. The substance is a glycoprotein with a carbohydrate content of 90%, a pI of 4.5, and a molecular weight of 130,000. The substance was inactivated by 2-mercaptoethanol and proteolytic enzymes but not by glycolytic enzymes. The substance formed a complementary complex having no biological activity when mixed with α-agglutination substance from the wall or cytoplasm of α-cells in vitro.
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  • 51
    ISSN: 1432-072X
    Keywords: Carbon catabolite inactivation ; Yeast ; Malate dehydrogenase ; Fructose-1,6-bisphosphatase ; Glycolytic block mutants
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    Topics: Biology
    Notes: Abstract Experimental conditions have been elaborated to test for reversibility of the malate dehydrogenase inactivation (E.C.1.1.1.37) after addition of glucose to derepressed yeast cells. Malate dehydrogenase inactivation was shown to be irreversible at all stages of inactivation. In contrast fructose-1,6-bisphosphatase inactivation (E.C.3.1.11) remained reversible for at least 30 min after addition of glucose. Rapid reversible inactivation of fructose-1,6-bisphosphatase and irreversible inactivation of malate dehydrogenase were additionally investigated in glycolytic block mutants. Normal inactivation kinetics were observed in mutants without catalytic activity of phosphoglucoseisomerase (E.C.5.3.1.9), phosphofructokinase (E.C.2.7.1.11), triosephosphate isomerase (E.C.5.3.1.1) and phosphoglycerate kinase (E.C.2.7.2.3). Hence, neither type of inactivation depended on the accumulation of any glucose metabolite beyond glucose-6-phosphate. Under anaerobic conditions irreversible inactivation was completely abolished in glycolytic block mutants. In contrast rapid reversible inactivation was independent of energy provided by respiration or fermentation. Reversibility of fructose-1,6-bisphosphatase inactivation was tested under conditions which prevented irreversible malate dehydrogenase inactivation. In these experiments, fructose-1,6-bisphosphatase inactivation remained reversible for at least 120 min, whereas reversibility was normally restricted to about 30 min. This indicated a common mechanism between the irreversible part of fructose-1,6-bisphosphatase inactivation and irreversible malate dehydrogenase inactivation.
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  • 52
    ISSN: 1432-072X
    Keywords: Induction ; Catabolic repression ; galactose metabolism ; Yeast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Induction and repression kinetics of alphagalactosidase, galactose uptake system and Leloir pathway enzymes were studied in chemostat cultures by changing the medium feed from glucose (11 mM) to glucose and galactose (11 mM; 17 mM respectively) in the induction experiments; and from galactose (11 mM) or (111 mM) to galactose plus glucose (83 mM) in the repression experiments. Basal levels of alpha-galactosidase and glucose uptake could be estimated in glucose-limited yeast cells, but it was not possible to detect any glactose pathway enzyme activity. In the repression experiments under galactose-limited or galactose-sufficient yeast cells, alpha-galactosidase and galactokinase decayed with K d=-0.21h-1=-D; that is, synthesis of these enzymes ceased (catabolite repression). In contrast transferase and epimerase activities and galactose uptake, decreased with K d values of-0.33 and-0.54h-1, showing that these activities were also subject to catabolite inactivation.
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  • 53
    ISSN: 1432-072X
    Keywords: Nitrobacter hamburgensis ; Nitrite oxidoreductase ; Nitrate reductase ; Molybdenum iron-sulfur protein ; Ultrastructure
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    Topics: Biology
    Notes: Abstract Nitrite oxidoreductase, the essential enzyme complex of nitrite oxidizing membranes, was isolated from cells of the nitrifying bacterium Nitrobacter hamburgensis. The enzyme system was solubilized and purified in the presence of 0.25% sodium deoxycholate. Nitrite oxidoreductase oxidized nitrite to nitrate in the presence of ferricyanide. The pH optimum was 8.0, and the apparent K m value for nitrite amounted to 3.6 mM. With reduced methyl-and benzylviologen nitrite oxidoreductase exhibited nitrate reductase activity with an apparent K m value of 0.9 mM for nitrate. NADH was also a suitable electron donor for nitrate reduction. The pH optimum was 7.0. Treatment with SDS resulted in the dissociation into 3 subunits of 116,000, 65,000 and 32,000. The enzyme complex contained iron, molydbenum, sulfur and copper. A c-type cytochrome was present. Isolated nitrite oxidoreductase is a particle of 95±30 Å in diameter.
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  • 54
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    Archives of microbiology 134 (1983), S. 270-275 
    ISSN: 1432-072X
    Keywords: Candida utilis ; Brettanomyces intermedius ; Yeast ; Glycolysis ; Vacuole ; Cytoplasm ; Phosphate compartmentation ; Phosphate transport ; Polyphosphate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract 31P NMR was used to study the dynamics of phosphate pools during substrate utilization by aerobic and anaerobic suspensions of the yeast Candida utilis and by aerobic suspensions of the yeast Brettanomyces intermedius. In both yeast, the cytoplasmic pH was monitored; in C. utilis also the vacuolar pH could be measured. When glucose was used as a substrate for C. utilis, the vacuolar store of inorganic phosphorus (both orthophosphate and polyphosphate) was mobilized to replenish cytoplasmic phosphate which had become very low due to the build-up of high sugar phosphate levels. The hydrolysis of polyphosphate was glucose-dependent; it did not occur with ethanol as the substrate. After glucose depletion resynthesis of polyphosphate occurred only under aerobic conditions; anaerobic C. utilis cells continued to hydrolyze vacuolar polyphosphate. This difference between the aerobic and anaerobic suspension could be related to differences in cellular ATP levels. When ethanol was employed as a substrate, both Candida utilis and Brettanomyces intermedius exhibited a substantial increase in polyphosphate levels. These observations suggested a dual role for polyphosphate in yeasts both as a phosphate and an energy store. The cytoplasmic pH in C. utilis displayed characteristic responses to metabolic changes during glucose degradation. B. intermedius experienced a strong cytoplasmic acidification upon ethanol utilization due to acetic acid formation. The mechanism of transport of Pi across the vacuolar membrane in C. utilis appeared to be different from that reported for the plasma membrane.
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  • 55
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    Archives of microbiology 134 (1983), S. 295-298 
    ISSN: 1432-072X
    Keywords: Actinomycetes ; Streptomyces thermoviolaceus ; Sporogenesis ; Spore ornamentation ; Cupular knobs ; Sheath ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The sporogenesis of aerial spores in Streptomyces thermoviolaceus corresponded to a common sporulation type in the genus. The sporulation septum was composed of an outer ring-shaped constriction wall and an inner interspace septum arising by the inwards growth of a double annulus. In mature spores the wall was composed of two layers, the outer one was part of the parent hyphal wall and septum material, the inner one was formed de novo. The spore chains were enclosed by the thin breakable sheath containing small rod-like elements. The ornamentation in the form of knobs, which were a characteristic feature of the species originated from the sheath. The knobs were hemispherical particles with an inner electron dense core and an outer electron transparent shell. The term “cupular knobs” was suggested for this type of tuberculate ornamentation. Frequently, the knobs became detached from the surface in which case the inner core separated easily from the shell.
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  • 56
    ISSN: 1432-072X
    Keywords: Cyanobacteria ; Ultrastructure ; Nitrogen fixation ; Water stress ; Taxonomy ; DNA ; Plasmids
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    Topics: Biology
    Notes: Abstract Two strains of desiccation-tolerant coccoid cyanobacteria, Chroococcus S24, a marine form, and Chroococcus N41, a cryptoendolith isolated from a hot-desert rock, have been characterized. The mol % DNA base compositions of the strains are 47.1 and 48.9% respectively. Plasmid DNA was not detected in either strain. The pigment contents and nutritional characteristics of the strains are identical. Both lack phycoerythrinoid pigments and, in culture, behave as slow-growing halotolerant marine forms with elevated requirements for Na+, Cl−, Mg2+ and Ca2+. Sucrose was the only carbon source of those tested that supported photoheterotrophic growth. Each strain synthesizes nitrogenase under anaerobic conditions but not in air. Morphologically the two strains are indistinguishable. They are considered to be independent isolates of the same cyanobacterial species. Chroococcus N41 was studied in detail with the electron microscope. When brought to equilibrium at matric water potentials of-168 MPa and lower (to-673 MPa=c0.12a w) the protoplast shrinks, but the cells maintain the same size and diameter as those at-2,156 kPa (MN medium; control); the sheath expands and remains attached to the cell wall outer membrane by fibrils. The cell wall, cell membrane, thylakoid membranes, cyanophycin granules and carboxysomes appeared intact in desiccated cells.
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  • 57
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    Journal of chemical ecology 10 (1984), S. 1425-1433 
    ISSN: 1573-1561
    Keywords: Social wasp ; Polistes exclamans ; Polistes fuscatus ; Vespidae ; Hymenoptera ; venom ; alarm pheromone ; alarm behavior
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The venoms ofPolistes exclamans andP. fuscatus elicit alarm behavior and attract attacking wasps. The response is not species specific, for both hetero- and conspecific venoms elicit similar responses in both species. A test in a wind tunnel provided no support for the hypothesis that alarmed wasps release an alarm pheromone on the nest.
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  • 58
    ISSN: 1573-1561
    Keywords: Kairomone ; 2-acylcyclohexane-1,3-diones ; ovipositionEphestia kuehniella Zeller [syn.Anagasta kuehniella (Zeller)] ; Lepidoptera ; Pyralidae ; Nemeritis canenscens (Grav.) [syn.Venturia canescens (Grav.)] ; Hymenoptera ; Ichneumonidae
    Source: Springer Online Journal Archives 1860-2000
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    Notes: Abstract The relative activities of sixteen 2-acylcyclohexane-1,3-diones from the larval mandibular glands ofEphestia (=Anagasta) kuehniella Zeller in causing the parasiteNemeritis (=Venturia) canescens (Grav.) to make oviposition movements are reported.
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  • 59
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    Journal of chemical ecology 10 (1984), S. 1787-1798 
    ISSN: 1573-1561
    Keywords: Coleoptera ; Curculionidae ; Otiorhynchus sulcatus (F.) ; black vine weevil ; Ericaceae ; Rhododendron ; trichomes ; glandular scales ; essential oils ; volatiles ; plant resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Glandular scales on selected lepidote rhododendron species varied in density from 109 ± 13 to 4180 ± 60/cm2 of leaf surface. Globules contained within the scales stained with Sudan IV, a lipophilic dye. Essential oil contents of the scales varied with species from 24 ± 8 to 151 ± 35 ng/scale. Black vine weevil [(Otiorhynchus sulcatus (F.)] feeding on leaves from a sample of rhododendron species was inversely related to leaf essential oil content, and weevil feeding on membrane filters was inhibited by application of essential oil extracts from leaves of most lepidote rhododendrons tested. Results suggest that the glandular scales of the lepidote rhododendrons function, at least in part, in plant defense against insects.
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  • 60
    ISSN: 1573-1561
    Keywords: Leptopilinaheterotoma ; Hymenoptera ; Eucoilidae ; Saccharomyces cerevisiae ; host-habitat searching ; chemoreception ; fermentation products ; ethanol ; ethyl acetate ; acetaldehyde
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Chemical stimuli play an important role in the process of searching for a host habitat by parasitic wasps. Volatile compounds originating from host habitats and/or hosts are the cues that enable such a location.Leptopilina heterotoma, a larval parasite ofDrosophila, is attracted to the food of its host, baker's yeast. Analysis of the fermentation products of baker's yeast, using a mass spectrometer, and olfactometer studies indicate that three fermentation products of this yeast, the main component of the host habitat in our laboratory, attractL. heterotoma: ethanol (5%), ethyl acetate (10−2, 10−3%), and acetaldehyde (1%). A combination of these three compounds, however, cannot compete with baker's yeast in attracting the parasites. Thus other factors, such as different compounds, concentrations, and/or combinations, also, play a role and remain to be tested.Leptopilina heterotoma does not use host-related olfactory cues in long-distance habitat location as it cannot distinguish between host habitat and host habitat with hosts.
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  • 61
    ISSN: 1573-1561
    Keywords: Pine sawflies ; Neodiprion spp. ; Hymenoptera ; Diprionidae ; electroantennogram ; sex pheromone ; isomers ; jack pine ; Pinus banksiana ; diprionol ; 3,7-dimethylpentadecan-2-ol ; acetate ; propionate
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Several species of monophagous jack pine sawflies (Hymenoptera: Diprionidae) were tested in the field and by electroantennograms (EAG) for activity toward the optical isomers of a pine sawfly sex pheromone, the acetate and propionate esters of 3,7-dimethylpentadecan-2-ol.Neodiprion rugifrons andNeodiprion dubiosus were attracted to a mixture of the propionate esters of the 2S,3R,7R and 2S,3R,7S isomers, whereasNeodiprion swainei was attracted to the 2S,3S,7S propionate isomer. Samples containing the 2S,3R,7S propionate isomer elicited the strongest EAG responses in these three species andNeodiprion nigroscutum. The 2S,3S,7S propionate isomer was equally active (EAG) in the case ofN. swainei.
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  • 62
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    Journal of chemical ecology 10 (1984), S. 723-752 
    ISSN: 1573-1561
    Keywords: Ips typographus ; pheromone ; release ; recapture ; diffusion ; model ; Coleoptera ; Scolytidae ; trap ; marking ; dispersal
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The movement of bark beetles near an attractive pheromone source is described in terms of mathematical models of the diffusion type. To test the models, two release experiments involving 47,000 marked spruce bark beetles [Ips typographus (L.)] were performed. The attractive source was a pheromone trap, surrounded by eight concentric rings with eight passive trap stations on each ring. Captures were recorded every 2–10 minutes for the pheromone trap and once for the passive traps. The models were fitted to the distribution in time of the central pheromone trap catch and to the spatial distribution of catch among the passive traps. The first model that gives a reasonable fit consists of two phases: Phase one—After release the beetles move according to a diffusion process with drift towards the pheromone trap. The strength of the drift is inversely proportional to the distance from the traps. Phase two—those beetles attracted to, but not caught by, the pheromone trap are no longer influenced by the pheromone, and their movement is described by a diffusion process without drift. In phase two we work with a loss of beetles, whereas the experiment seems to indicate that the loss of beetles in phase one is negligible. As a second model, the following modification of phase one is considered: After release the beetles move according to a diffusion process without drift, until they start responding to the pheromone (with constant probability per unit time), whereafter they start moving according to a diffusion process with drift. This study, like other release experiments, shows that the efficiency of the pheromone trap is rather low. What is specific for the present investigation is that we try to explain this low efficiency in terms of dynamic models for insect movement. Two factors seem to contribute: Some beetles do not respond to pheromone at all, and some beetles disappear again after having been close to the pheromone trap. It also seems that the motility of the beetles decreased after they ceased responding to the pheromone. Furthermore, the data lend some support to the hypothesis that flight exercise increases the response of the beetles to pheromone.
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  • 63
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    Journal of chemical ecology 10 (1984), S. 759-769 
    ISSN: 1573-1561
    Keywords: Ips typographus ; Dendroctonus micans ; Coleoptera ; Scolytidae ; exo-brevicomin ; (+)-ipsdienol ; single-cell recordings ; interspecific attraction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Olfactory receptor cells were studied electrophysiologically inIps typographus andDendroctonus micans. The investigation revealed cells which were keyed to pheromone compounds characteristic of the reciprocal genus. Thus, cells keyed toexo-brevicomin were found inI. typographus, whereas cells keyed to (+)-ipsdienol were present inD. micans. Laboratory behavioral tests indicated an attractive effect of the two compounds on beetles of the reciprocal genus. InI. typographus the effect ofexo-brevicomin predominantly concerned males and enhanced their response to the pheromone “ipslure.” It is suggested thatexo-brevicomin serves as an interspecific attractant forI. typographus, which may be guided by pheromone compounds of the reciprocal genus in finding suitable breeding material. The function of (+)-ipsdienol inD. micans is more uncertain. It may be either a pheromone or an interspecific messenger.
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  • 64
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    Journal of chemical ecology 10 (1984), S. 1759-1785 
    ISSN: 1573-1561
    Keywords: Cotton boll weevil ; Anthonomus grandis ; Coleoptera ; Curculionidae ; pheromone ; kairomone ; plant odor ; olfaction ; electroantennogram ; attractant ; host plant ; green leaf volatiles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Electroantennogram (EAG) techniques were utilized to measure the antennal olfactory responsiveness of adult boll weevils,Anthonomus grandis Boh. (Coleoptera: Curculionidae), to 38 odorants, including both insect and host plant (Gossypium hirsutum L.) volatiles. EAGs of both sexes were indicative of at least two receptor populations: one receptor population primarily responsive to pheromone components and related compounds, the other receptor population primarily responsive to plant odors. Similar responses to male aggregation pheromone components (i.e., compounds I, II, and III + IV) were obtained from both sexes, but females were slightly more sensitive to I. Both sexes were highly responsive to components of the “green leaf volatile complex,” especially the six-carbon saturated and monounsaturated primary alcohols. Heptanal was the most active aldehyde tested. More acceptors responded to oxygenated monoterpenes than to monoterpene hydrocarbons. β-Bisabolol, the major volatile of cotton, was the most active sesquiterpene. In general, males, which are responsible for host selection and pheromone production, were more sensitive to plant odors than were females. In fact, males were as sensitive to β-bisabolol and heptanal as to aggregation pheromone components. Electrophysiological data are discussed with regard to the role of insect and host plant volatiles in host selection and aggregation behavior of the boll weevil.
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  • 65
    ISSN: 1573-1561
    Keywords: Ips typographus ; spruce bark beetle ; Coleoptera ; Scolytidae ; 2-methyl-3-buten-2-ol ; ipsenol ; cis-verbenol ; ipsdienol ; trans-verbenol ; verbenone ; myrtenol ; trans-myrtanol ; 2-phenylethanol ; ß-isophorone
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Ips typographus beetles were collected in the field, separated into eight attack phases (from beetles walking on the trunk of a tree under attack to those excavating gallery systems with a mother gallery longer than 4 cm), and immediately frozen in liquid nitrogen. 2-Methyl-3-buten-2-ol,cis- andtrans-verbenol, verbenone, myrtenol, trans-myrtanol, ipsenol, ipsdienol, and 2-phenylethanol were quantified from excised hindguts against an internal standard, heptyl acetate, in the extraction solvent. Methylbutenol, the pinene alcohols, and 2-phenylethanol showed the same pattern of variation between attack phases in males, with the largest amounts present before accepting females and then a fast decline. Ipsenol and ipsdienol were not detected in males before the females were accepted, and the amounts increased when the females start their egg laying. Verbenone occurred only in trace amounts. The beetles were sampled from five Norway spruce trees (Picea abies) of differing resin flow. The correlations between the nine pheromone components and five major host monoterpenes in the gut showed that the variation in the amount of methyl-butenol, ipsenol, and ipsdienol could not be explained by the variation in the amounts of host monoterpenes. In contrast over 80% of the quantitative variation ofcis-verbenol,trans-verbenol, and myrtenol was explained by the amount of α-pinene. The nine pheromone components from 36 individual males were also quantified. Both methylbutenol andcis-verbenol showed a large variation in both amounts and proportions. Females containedtrans-verbenol and traces of most other components found in males. When accepted by the male, they also contained a female-specific compound, β-isophorone. Behavioral and biosynthetic implications of the results are discussed.
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  • 66
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    Journal of chemical ecology 9 (1983), S. 1449-1464 
    ISSN: 1573-1561
    Keywords: Coleoptera ; Dytiscidae ; Agabus seriatus ; Agabus obtusatus ; defensive secretions ; steroids ; regeneration ; pygidial glands ; prothoracic glands ; cholesterol
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The defensive secretions of the dytiscid species,Agabus seriatus (Say) andAgabus obtusatus (Say), were qualitatively and quantitatively analyzed by high-pressure liquid chromatography. The intrinsic ability ofA. Seriatus andA. Obtusatus to regenerate their prothoracic gland defensive secretions under laboratory conditions was determined by analyzing the secretions every seventh day for five weeks. Both beetles regenerated ∼ 80% of their prothoracic gland components within two weeks.A. seriatus was injected with [4-14C]cholesterol and after a three-week regeneration period 7.5% of the14Clabel was found in the steroidal defensive secretion from the prothoracic glands.
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  • 67
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    Journal of chemical ecology 9 (1983), S. 1533-1541 
    ISSN: 1573-1561
    Keywords: Aggregation pheromone ; attractant ; 1-methylcyclohex-2-en-1-ol ; Dendroctonus pseudotsugae ; Coleoptera ; Scolytidae ; bark beetle ; Douglas-fir beetle ; 3-methylcyclohex-3-en-1-ol ; Pseudotsuga menziesii
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    Notes: Abstract 1-Methylcyclohex-2-en-1-ol (1,2-MCH-ol) was synthesized, identified as a compound found in volatiles of the female Douglas-fir beetle, and shown by bioassays to be an aggregation pheromone. 1,2-MCH-ol matches in both GC retention index and mass spectrum a compound released by the female after feeding. 3,3-MCH-ol was also synthesized as a candidate compound; its mass spectrum is presented because published mass spectra are incorrect for this compound. Synthetic 1,2-MCH-ol increased arrestment and stridulation of males in olfactory walkways and increased trap catches of flying beetles. Males were more responsive to 1,2-MCH-ol than females.
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  • 68
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    Journal of chemical ecology 9 (1983), S. 181-190 
    ISSN: 1573-1561
    Keywords: Coleoptera ; Buprestidae ; Cerambycidae ; Cleridae ; Scolytidae ; wood-boring beetles ; bark beetles ; ethanol ; host attractants ; hardwood tree insects
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Ethanol, methanol, acetone, and acetaldehyde—chemicals identified in the inner bark of living trees—were used to bait vane traps placed in crowns of oak trees in Connecticut. Ethanol-baited traps caught more cerambycid, scolytid, and clerid beetles than unbaited traps. Buprestidae were not attracted to ethanol. Acetaldehyde and acetone were not attractive to any family. A mixture of ethanol, methanol, and acetaldehyde was no more attractive than ethanol alone. The vane traps were very effective at catching Cerambycidae and Scolytidae, but ineffective compared to sticky panels at catching Buprestidae.
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  • 69
    ISSN: 1573-1561
    Keywords: Coleoptera ; Scolytidae ; bark beetle ; Ips paraconfusus ; aggregation pheromone ; enantiomer ; electrophysiology ; electroantennogram ; interruption ; allomone
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    Notes: Abstract Antennae of male and femaleIps paraconfusus were equally and highly sensitive to their male-produced, multicomponent aggregation pheromone. Female and male antennae were highly sensitive to the pheromonal component, (S)-(−)-ipsenol, but essentially insensitive to its antipode, (R)-(+)-ipsenol. Further, female and male antennae were more sensitive to the pheromonal component, (S)-cis-verbenol, than to its antipode, (R)-cis-verbenol. Dramatic sexual dimorphism in chiral sensitivity to the ipsdienol enantiortiers was found, with female antennae being more sensitive to the conspecific pheromonal enantiomer, (S)-(+)-ipsdienol, and male antennae being more sensitive to the antipode, (R)-(−)-ipsdienol. Since (R)-(−)-ipsdienol is the principal pheromone of CaliforniaIps pini and interruptsI. paraconfusus aggregation, male antennae appear to be more sensitive to an interspecific allomone than a conspecific pheromone. Of the conspecific pheromonal enantiomers, both male and female antennae were most sensitive to (S)-(+)-ipsdienol, intermediately sensitive to (S)-(−)-ipsenol, and least sensitive to (S)-cis-verbenol. However, when enantiomeric sensitivities were compared to the estimated concentrations of these components in the natural pheromone, (S)-(~)-ipsenol tended to equal or approach the potency of (S)-(+)-ipsdienol as an antennal stimulant, while antennal responsiveness to (S)-cis-verbenol was dramatically less than for the other two pheromonal components. The behavioral implications of such physiological sensitivities are discussed in regard to perception of multicomponent synergistic pheromones and the relative efficacy of each component as an orientation cue.
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  • 70
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    Journal of chemical ecology 9 (1983), S. 585-606 
    ISSN: 1573-1561
    Keywords: Coleoptera ; Scolytidae ; bark beetle ; Ips paraconfusus ; pheromone ; enantiomer ; electrophysiology ; electroantennogram ; interruption ; allomone
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The antennal sensitivities of both male and femaleIps paraconfusus were found generally to be greatest for conspecific aggregation pheromones (ipsdienol, ipsenol); intermediate for an additional conspecific pheromone (cis-verbenol), an aggregation synergist (2-phenylethanol), and pheromones/allomones of sympatric species (trans-verbenol, verbenone, and frontalin); and lowest for both host terpenes (alpha-pinene and myrcene) and other bark beetle-produced odorants (exo-brevicomin and linalool). Of the enantiomeric compounds tested, antennae of both sexes did not differ in sensitivity between thetrans-verbenol enantiomers at low dosage levels; but at higher dosages, the conspecific-produced enantiomer, (1R,4S,5R)-(+)-trans-verbenol, elicited larger mean EAG responses than its antipode, (1S,4R, 5S)-(−)-trans-verbenol. At the mid-dosage range, female antennae tended to be slightly more responsive to (S)-(−)-verbenone than to (R)-(+)-verbenone, while male antennae were equally responsive to stimulations by either verbenone enantiomer. In field bioassays there was a large and significant reduction in trap catches ofI. paraconfusus on traps where the (S)-(−)- or (R)-(+)-enantiomers of verbenone were evaporated beside logs containing boring conspecific males. Only when the (S)-(−)-enantiomer of verbenone was evaporated beside logs containing boring males did the sex ratio ofI. paraconfusus trapped shift from female-dominated to male-dominated attraction. Thus both physiological and behavioral data suggest a differential chiral sensitivity of female beetles for the verbenone enantiomers. The relative sensitivities between different chiral compounds derived from one or the other of the common precursoral host terpenes, (S)-(−)- and (R)-(+)alpha-pinene or myrcene, are discussed.
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  • 71
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    Journal of chemical ecology 9 (1983), S. 13-31 
    ISSN: 1573-1561
    Keywords: Aleochara curtula (Goeze) ; Coleoptera ; Staphylinidae ; tergal gland secretion ; defense ; mating stimulants ; female sex pheromone ; hydrocarbons ; n-aldehydes ; substituted 1 ; 4-benzoquinones
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract At high concentrations, the defensive tergal gland secretion (TGS)3 of the staphylinid beetle,Aleochara curtula, inhibits the male copulatory response (grasping with parameres). Inhibitory chemicals, for which a function as alarm substances is assumed, aren-undecane, 1-undecene,n-dodecanal, toluquinone, and 2-methoxy-3-methyl-1,4-benzoquinone. When emitted in small amounts, however, the TGS releases the male grasping response. The main components with aphrodisiac effect are (Z)-4-tridecene,n-dodecanal, and (Z)-5-tetradecenal. These supplementary mating stimulants, which are not sex specific, work synergistically with the aphrodisiac female sex pheromone from thé epicuticular lipids and are discussed as alerting pheromones of short-term effect. Antennal movements of resting males as an indication of the recognition of a female and the approach to the mate are released at somewhat longer distances, when the TGS is additionally present.
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  • 72
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    Journal of chemical ecology 9 (1983), S. 57-65 
    ISSN: 1573-1561
    Keywords: Honeybee ; Apis mellifera ; alarm pheromone ; acetate ; alcohol ; Hymenoptera ; Apidae ; sting
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Twelve compounds identified from honeybee,Apis mellifera L., sting extracts were evaluated in a standardized laboratory test for their effectiveness in eliciting an alarm response from caged worker honeybees. Two-1-decanol and phenol-were judged ineffective as alarm pheromones. The other ten-1-butanol, isopentyl acetate, isopentyl alcohol, 1-hexanol, 2-heptyl acetate, 2-heptanol, 1-octanol, 1-acetoxy-2-octene, 2-nonyl acetate, and 1-acetoxy-2-nonene-produced alarm responses of similar speed and intensity. Three non-sting-derived compounds-β-ionone, methyl benzoate, andtrans-cinnamaldehyde—caused weak or no responses, indicating that the responses were not simply a reaction to concentrated odoriferous substances.
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  • 73
    ISSN: 1573-1561
    Keywords: Scolytus multistriatus ; S. scolytus ; Coleoptera ; Scolytidae ; elm bark beetle ; multistriatin stereoisomers ; Dutch elm disease ; aggregation pheromone ; field responses ; attractant baits
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The field responses of English populations of the Dutch elm disease vectors,Scolytus multistriatus andS. scolytus to baits containing 4-methyl-3-heptanol, a host synergist [(−)-α-cubebene or (−)-limonene] and (±)-α-, (+)-β-, (−)-β-, (±)-γ-, or (±)-δ-multistriatin were examined. (±)-α-Multistriatin, released at 5–10 μg/day, enhanced the response ofS. multistriatus to baits containing 4-methyl-3-heptanol and either of the host synergists but had no effect on the capture ofS. scolytus. The release of larger amounts (57 or 365 μg/day) of (±)-α-multistriatin interrupted the response of both species to the 4-methyl-3-heptanol baits. It appears that α-multistriatin has multiple functions as a behavior-modifying substance for the two beetles. The (+)-β-, (−)-β-, (±)-γ-, and (±)-δ-multistriatins were inactive when released at 5–10 μg/day. The results of these field experiments suggest that one bait can be formulated to capture both species.
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  • 74
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    Journal of chemical ecology 9 (1983), S. 105-111 
    ISSN: 1573-1561
    Keywords: Pogonomyrmex badius ; Hymenoptera ; Formicidae ; oleic acid ; social context ; midden ; pheromone ; harvester ant ; necrophoric
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    Notes: Abstract The response of the southern harvester ant,Pogonomyrmex badius, to oleic acid was found to depend on social context. Social context was specified as the number of ants engaging in each of five categories of behavior. When a large percentage of the colony is doing midden work or nest maintenance, papers treated with oleic acid are taken to the midden, as previously reported. However, when a large percentage of the colony is foraging or convening, treated papers are taken into the nest as if they were food items.
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  • 75
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    Keywords: Larch sawfly ; Pristiphora erichsonii (Hartig) ; Hymenoptera ; Tenthredinidae ; feeding behavior ; nutritional indices ; abietic acid ; dehydroabietic acid ; neoabietic acid ; isopimaric acid ; sandaracopimaric acid ; resin acids
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    Notes: Abstract Diterpene resin acids significantly affect consumption rates, feeding efficiencies, and growth rates of the larch sawfly,Pristiphora erichsonii (Hartig) when topically applied to their natural food, tamarackLarix laricina (DuRoi) K. Koch. Abietic acid, neoabietic acid, dehydroabietic acid, and isopimaric acid significantly reduced consumption rates, feeding efficiencies, and growth rates. Sandaracopimaric acid reduced growth and efficiency but did not influence consumption rate. Two-way analysis of variance indicates a significant interaction between chemical and concentration for growth rate, feeding efficiency, and consumption rate. This interaction indicates that increasing chemical concentrations do not influence the larch sawfly in a uniform manner, supporting the concept of concentration-dependent biological activity of allelochemics.
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  • 76
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    Journal of chemical ecology 9 (1983), S. 397-422 
    ISSN: 1573-1561
    Keywords: Cruciferae ; chemical defense ; gradient ; glucosinolate ; herb-ivory ; insect-plant interactions ; Cardamine cordifolia ; Coleoptera ; Chrysomelidae ; Homoptera ; Psyllidae
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Insect use of native crucifers may be related to patterns in mustard oil content. Consequently, in 1979 we measured glucosinolate content of Rocky Mountain bittercress,Cardamine cordifolia (Cruciferae), using paper and gas chromatography, in relation to: plant organ, phenology, elevation, habitat, leaf position and weight, and plant consumption by two adapted insect herbivores. Results for each are as follows. (1) The predominant constituent in all vegetative organs was 2-butylglucosinolate; concentration of isothiocyanate-yielding glucosinolates (IYG) was highest in roots (1.11 mg/gfr. wt) and lowest in stems (0.07 mg/g). (2) Concentration of IYG appeared to be higher in plants lacking oxazolidinethione-yielding glucosinolates (OYG) than in those with OYG. (3) Terminal cauline leaves had a higher content of IYG than leaves in other positions on a plant. (4) Heavy leaves had significantly higher concentrations of IYG than did lighter leaves. (5) IYG concentrations were not directly related to elevation. (6) Leaves of plants occurring naturally in the sun had concentrations of IYG similar to those of plants in the usual shaded habitat. However, experimental removal of overhanging willows caused a significant, stress-induced increase in IYG concentrations. Finally, (7) feeding by two adapted herbivores, chrysomelids and psyllids, was associated with lower, rather than higher, IYG concentrations. The results demonstrate significant variation in glucosinolate content in a native crucifer and suggest that some of this variation can be partitioned in relation to the ecological and environmental axes examined.
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  • 77
    ISSN: 1573-1561
    Keywords: Cryptolestes ferrugineus ; rusty grain beetle ; aggregation ; pheromone ; macrolide ; (E,E)-4,8-dimethyl-4,8-decadien-10-olide ; (3Z,11S)-3-dodecen-11-olide ; (Z,Z)-3,6-dodecadien-11-olide ; (Z)-5-tetradecen-13-olide ; 11-dodecanolide ; 4-nonanolide ; Coleoptera ; Cucujidae
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Two synergistic macrolide aggregation pheromones were isolated from Porapak Q-trapped volatiles obtained from the frass ofCryptolestes ferrugineus (Stephens). These compounds were identified as (E,E)-4,8-dimethyl-4,8-decadien-10-olide (I) and (3Z,11S)-3-dodecen-11-olide (II) and given the trivial names ferrulactone I and II, respectively. Analysis of captured volatiles from separated male and female adults disclosed that the pheromones are male-produced. Additional macrolides were identified in frass volatiles, but were devoid of any pheromonal activity. The structures of I and II were confirmed by comparison with synthetic materials.
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  • 78
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    Journal of chemical ecology 9 (1983), S. 657-672 
    ISSN: 1573-1561
    Keywords: Pseudoplusia includens ; soybean looper ; Lepidoptera ; Noctuidae ; Epilachna varivestis ; Mexican bean beetle ; Coleoptera ; Coccinellidae ; feeding preferences ; nutrition ; food utilization ; host-plant resistance ; induced resistance ; glyceollin ; isoflavonoids ; soybean ; phytoalexins
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Effects of soybean phytoalexins on the feeding of the soybean looper and Mexican bean beetle were investigated to test the hypothesis that phytoalexins might be a defense mechanism of plants against insects as well as against pathogens. Short-term behavioral responses to the phytoalexins were analyzed using dual-choice tests with phytoalexin-rich and phytoalexin-poor (control) tissues. Phytoalexin production was elicited with ultraviolet radiation. Results from the dual-choice tests indicated that 6th instar soybean looper larvae fed equally on the control and phytoalexin-rich tissues. Feeding by adult and 4th instar Mexican bean beetles, however, was strongly deterred by the phytoalexins as evidenced by “single-bite” mandible scars on the phytoalexin-rich cotyledon discs. Nutritional effects of the isoflavonoid phytoalexin glyceollin on early instar soybean looper larvae were tested by incorporating the phytoalexin into an artificial medium at a level of 1% dry weight (0.15% fresh weight). The larvae were reared for 7 days from emergence on diets of control and glyceollin-containing media. Although survival on the glyceollin diets was initially less than on the control diets, under the experimental conditions glyceollin had no significant effect on the growth, development, or subsequent survival of the larvae. Efficiency of food utilization (ECI) was reduced, indicating that the phytoalexins may be a mild digestibility-reducing factor for the loopers. Implications of the results for host-plant resistance are discussed.
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  • 79
    ISSN: 1573-1561
    Keywords: Xylocopa hirsutissima ; Vespa orientalis ; Camponotus ; Hymenoptera ; Xylocopidae ; Vespidae ; Formicidae ; pheromone ; allomone ; chemical preparation ; cis-3,6-dimethyltetrahydro-2-pyrone ; 6-n-undecyltetrahydro-2-pyrone ; 6-n-pentyl-5,6-dihydro-2-pyrone ; lactones
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Simple preparations ofcis-3,6-dimethyltetrahydro-2-pyrone, VII,6-n-undecyltetrahydro-2-pyrone, XVIIb, and 6-n-pentyl-5,6-dihydro-2-pyrone, XVIa, have been achieved. Products VII and XVIIb, respectively, are the major constituent of the pheromonal blend of a carpenter bee (Xylocopa hirsutissima) and the pheromone of the queens of the Oriental hornet (Vespa orientalis). The lactone XVIa is a suspected defensive allomone in two species of formicine ants of the genusCamponotus. All three compounds have been prepared from dehydroacetic acid, I, a cheap and industrially available starting material.
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  • 80
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    Journal of chemical ecology 9 (1983), S. 1353-1361 
    ISSN: 1573-1561
    Keywords: Canavanine ; Caryedes brasiliensis ; Coleoptera ; Bruchidae ; Dioclea megacarpa ; plant-insect interactions ; amino acids
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    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Larvae of the bruchid beetle,Caryedes brasiliensis (Bruchidae) have the ability to avoid significant incorporation ofl-canavanine, the guanidinooxy structural analog ofl-arginine, into de novo synthesized proteins. This ability is related to a highly discriminatory protein-synthesizing system which exhibits marked ability to avoid processing an array of nonprotein amino acids structurally related to arginine.
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  • 81
    ISSN: 1432-234X
    Keywords: Ultrastructure ; Gills ; Epithelial cells ; Polychaeta
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    Topics: Biology
    Notes: Summary The ultrastructure of gill epidermal cells of Diopatra neapolitana and their relationship with blood spaces are described. The existence of a basal infolding complex, related to the blood spaces, is also reported. A possible involvement of these cells in osmoregulation and ion interchange, apart from their well-known role in respiration, is suggested.
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    Theoretical and applied genetics 68 (1984), S. 305-309 
    ISSN: 1432-2242
    Keywords: Impatiens ; Microspore mitosis ; Plastid distribution ; Ultrastructure
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    Topics: Biology
    Notes: Summary This paper describes the unequal distribution of plastids in the developing microspores of Impatiens walleriana and Impatiens glandulifera which leads to the exclusion of plastids from the generative cell. During the development from young microspore to the onset of mitosis a change in the organization of the cytoplasm and distribution of organelles is gradually established. This includes the formation of vacuoles at the poles of the elongate-shaped microspores, the movement of the nucleus to a position near the microspore wall in the central part of the cell, and the accumulation of the plastids to a position near the wall at the opposite side of the cell. In Impatiens walleriana, the accumulated plastids are separated from each other by ER cisterns, and some mitochondria are also accumulated. In both Impatiens species, the portion of the microspore in which the generative cell will be formed is completely devoid of plastids at the time mitosis starts.
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  • 83
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    Theoretical chemistry accounts 63 (1983), S. 323-338 
    ISSN: 1432-2234
    Keywords: Dynamic systems ; Chaotic chemical reactions ; Kinetic logic ; Turbulence ; Belousov-Zhabotinskii reaction
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    Topics: Chemistry and Pharmacology
    Notes: Abstract Earlier approaches to the analysis of chemical dynamic systems using kinetic logic are refined to deal more effectively with systems having the two or more feedback circuits required for chaos. The essential kinetic features of such a system can be represented by a directed graph (called aninfluence diagram) in which the vertices represent the internal species and the directed edges represent kinetic relationships between the internal species. Influence diagrams characteristic of chaotic chemical systems have the following additional features: (1) They are connected; (2) Each vertex has at least one edge directed towards it and one edge directed away from it; (3) There is at least one vertex, called a turbulent vertex, with at least two edges directed towards it. From such an influence diagram a state transition diagram representing the qualitative dynamics of the system can be obtained using the following 4-step procedure: (1) A logical relationship is assigned at each turbulent vertex; (2) A local truth table is generated for each circuit in the influence diagram; (3) The local truth tables are combined to give a global truth table using the logical relationships at the turbulent vertices; (4) The global truth table is used to determine the corresponding state transition diagram using previously described methods. This refined procedure leads to a more restricted set of influence diagrams having the interlocking cycle flow topology required for chaos than the procedure described earlier. Systems with 3 internal species are examined in detail using the refined procedure. All systems with 3 dynamic variables shown in the simulation studies of Rössler to give chaotic dynamics correspond to influence diagrams which give inter-locking cycle (chaotic) flow topologies by the refined procedure. In addition, two models for the Belousov-Zhabotinskii reaction are examined using the refined procedure. The results are potentially informative concerning possible mechanisms for the limitation of the accumulation of autocatalytically produced HBrO2 (one of the internal species) during the course of this reaction.
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    Cell & tissue research 235 (1984), S. 309-318 
    ISSN: 1432-0878
    Keywords: Ascidian ; Gut ; Cell involution ; Ultrastructure ; Phagocytes
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    Topics: Biology , Medicine
    Notes: Summary Degenerative changes in the digestive tract of zooids of Botryllus schlosseri were studied by light and electron microscopy. Three main processes occurred in the tissues: contraction, involution and phagocytosis. The contraction of epidermis and peribranchial epithelium in which cytoplasmic microfilaments probably participate, seemed to have a special role in compressing the underlying organs. During contraction most of the body cavities collapsed, the branchial walls disintegrated and the fragments were rapidly taken up by large phagocytes. The gut epithelium retained its apparent continuity longer, though isolated phagocytes infiltrated it to eliminate single cells. Cell degeneration came about chiefly either through swelling and lysis of cells or through loss of water and condensation of cytoplasm and nucleus. The fate of all regressed tissues was to be engulfed and digested by wandering phagocytes. However, it was also observed that numerous cells of different epithelia could act as fixed phagocytes by engulfing cell debris and entire cells into heterophagic vacuoles.
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    Cell & tissue research 235 (1984), S. 347-356 
    ISSN: 1432-0878
    Keywords: Blastocyst ; Ultrastructure ; Pig
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Between days 8 and 11 of pregnancy spherical blastocysts from 0.3 to 10 mm in diameter were flushed from the uterine horns of Dutch Landrace pigs. A description of their ultrastructure is given, and the uptake of horseradish peroxidase and ferritin is demonstrated. The ultrastructure of the trophoblast was similar at all ages studied. The trophoblast which has many apical microvilli is able to take up and digest the macromolecules which were offered in the in vitro incubation medium. The hypoblast consists of flattened cells. In blastocysts 2 mm and larger, compact cells bearing microvilli are found below the embryoblast. Cell organelles indicating protein synthesis are found within hypoblast cells of such blastocysts. In the embryoblast, local concentrations of cell organelles are visible, indicating that differentiation has started. After the disappearance of Rauber's layer, which takes place when the blastocyst reaches a diameter of about 2 mm, superficial embryoblast cells develop short microvilli. The cells do not absorb ferritin or peroxidase but are dependent on the trophoblast for their food requirements. All cell layers in the blastocyst contain mitochondria that have characteristics of those found in steroidproducing cells. The significance of the uptake and digestion of macromolecules by trophoblast cells, the synthesis of protein by hypoblast cells and the possible synthesis of steroids is discussed with respect to the relationship between the cell layers of the blastocyst and in the context of conceptomaternal relationships.
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    Cell & tissue research 236 (1984), S. 365-372 
    ISSN: 1432-0878
    Keywords: Skeletal muscles ; Myofibrils ; Ultrastructure ; Exertion ; Man
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The fine structure of muscle fibres from m. vastus lateralis of nine healthy males (mean age 26 years) was investigated. Four individuals constituted non-exercised controls while five subjects participated in a two-months eccentric muscular training program. Specimens from the controls showed a well-preserved, regular myofibrillar band pattern while changes in the myofibrillar architecture were constantly found in specimens taken after the training program. These changes consisted of Z-band alterations, Z-bands being out of register, extra sarcomeres, Z-band extensions and bisected Z-bands. Between the separated Z-band halves, thin and thick myofilaments as well as abundant glycogen particles and/or ribosomes, were observed. Type-2 (fast-twitch) fibres were predominantly affected. Contrary to the controls the trained individuals constantly showed a greater variation in sarcomere lengths in Type-2 fibres than in Type-1 fibres. It is concluded that muscular work of high tension can induce fine-structural alterations. When repeated over a long period of time, extreme tension demands seem to initiate reorganization in the muscle fibres, predominantly in the, ultrastructurally defined, Type-2 fibres. This adaptation probably results in a better stretchability of the muscle fibres, reduces the risk for mechanical damage and brings about an optimal overlap between actin and myosin filaments.
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  • 87
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    Cell & tissue research 236 (1984), S. 393-397 
    ISSN: 1432-0878
    Keywords: Smooth muscle ; Salamander, Amphiuma ; Ultrastructure ; Stereology ; Volume: surface area ratio
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary An ultrastructural and stereological examination was performed on stomach smooth muscle of the salamander Amphiuma. This tissue has very large cells, ranging up to 12×1500 μm when relaxed. The extracellular space is 31% of the tissue volume, and the tissue contains 84.6% water. These values are similar to those of other amphibian and mammalian gastrointestinal smooth muscle. The cells possess the usual smooth muscle organelles. Thick, thin and intermediate filaments are present, along with membrane-associated and cytoplasmic dense regions. There is a well-developed sarcoplasmic reticulum and many microtubules. Caveolae are found in rows along the cellular surface; the caveolae increase the cellular surface area by about 70%. The ratio mean volume: surface area of the cells is 1.26 μm. This tissue appears to be typical of gastrointestinal smooth muscle, with the exception of the very large size of the cells.
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  • 88
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    Cell & tissue research 236 (1984), S. 619-628 
    ISSN: 1432-0878
    Keywords: Gastrotricha, freshwater ; Sperm, reduced ; Ultrastructure ; Spermatogenesis ; Temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Spermatogenesis and spermiogenesis in Lepidodermella squammata are confined to the postparthenogenic phase of the life cycle and coincide with developmental changes in the bilateral female gonads. Male stages are bilateral but asynchronous, in the lateral abdomen anterior to the female gonads. Maximum observed sperm production is two packets per side, or 64 sperm. Sperm formation occurs more rapidly at 27° C than at 20° C (p〈0.001), requiring as little as 1 day. Two spermatogonial mitotic divisions produce a clone of four primary spermatocytes connected by bridges (stage 1). Centrioles are absent. Development occurs within a cyst. Meiotic divisions produce 16 spermatids (stage 2), each containing a dense, elongate, tapered nucleus. Cytoplasmic membranes enclose one end of the nuclear rod, excluding all other organelles. Completion of this process results in stage 3, a packet of 16 sperm associated with one dense sphere, a modified ‘residual body’ containing cytoplasmic debris. The residual body then disappears, leaving the sperm packet of stage 4. Each mature sperm is a dense nuclear rod with surrounding membranes, lacking acrosome, mitochondrion, centrioles, and flagellum. Function of sperm has not been demonstrated. The spermatozoa are of a reduced type not previously described.
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  • 89
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    Cell & tissue research 236 (1984), S. 629-636 
    ISSN: 1432-0878
    Keywords: Oocytes, primary ; Gastrotricha, freshwater ; Ultrastructure ; Synaptonemal complex ; X-body
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Six small cells are present in each of the bilateral gonads of parthenogenically reproductive Lepidodermella squammata. Early in the extended postparthenogenic phase of the life history, these cells undergo limited proliferation followed by differentiation. Primary oocytes of three types are present 0.3 days after deposition of the final parthenogenic egg: small oocytes with presynaptic nuclei; intermediate oocytes with nuclei containing synaptonemal complexes; and larger oocytes with a germinal vesicle. Oocytes persist without further development at least until day four of the postparthenogenic phase. Older isolated animals may contain and even deposit an enlarged egg, but successful progeny does not result. Oocytes are located at the anterior pole of each of the bilateral gonads, adjacent to developing male tissues producing sperm. More posterior cells in the gonad are initially undifferentated in the postparthenogenic phase. Dorsal and central cells first show specialization for secretory activity, and by day four contain peripheral layers of RER and central accumulations of polymorphic secretion droplets. The posterior and ventral cells produce secretion droplets that aggregate into an enlarging bilobed structure called the X-body. Two or three cells in each gonad contribute secretions to the X-body, which is intracellular in a secondary syncytium formed by the contributing cells. Functions for the postparthenogenic gametes and for the X-body are not yet demonstrated.
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  • 90
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    Cell & tissue research 236 (1984), S. 693-697 
    ISSN: 1432-0878
    Keywords: Intermediate filaments ; Microtubules ; Caveolae ; Bullfrog ; Arachnoid mater ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the bullfrog, the meninges surrounding the central nervous system include an arachnoid mater that contains layers of cells with abundant intermediate filaments (IFs) having unique organizational characteristics. This membrane contains an inner lamina of cells that resemble fibroblasts and an outer lamina of flattened cells that are almost filled with IFs. The IFs of the outer arachnoid are arranged in compact, arching bundles that lie parallel to the outer surface of the central nervous system. Thus, sections cut tangentially to the membrane reveal bending of filament bundles, whereas transverse sections do not. In some cells bordering the subdural space, bundles of filaments are organized into highly-ordered spiral arrays. Attachments to the numerous desmosomes and, apparently, to the nuclear envelope suggest anchoring of cytoplasmic structures by the IF system. Microtubules occur primarily near the plasma membrane and the nucleus. Numerous caveolae also are associated with the plasma membrane. The unusual abundance, organization, and cytoplasmic relations of IFs in the bullfrog arachnoid suggest that this membrane may serve as an important model for study of fundamental cytoskeletal relations and function.
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  • 91
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    Cell & tissue research 237 (1984), S. 277-284 
    ISSN: 1432-0878
    Keywords: Swimbladder ; Teleost ; Cholinergic nerves ; Adrenergic nerves ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The general structure, ultrastructure and innervation of the swimbladder of the smooth toadfish, Tetractenos glaber, were examined with light-microscopic, fluorescence-histochemical, and transmission electron-microscopic techniques. The structure of the swimbladder is similar to that of other euphysoclists. Fluorescence histochemistry showed adrenergic fibres in both the secretory and resorptive areas of the swimbladder. Transmission electron microscopy revealed two morphologically distinct axon profiles type-I profiles containing many small, flattened vesicles; type-II profiles containing both large, granular vesicles and rounded, small clear vesicles in varying proportions. The gas-gland cells and surrounding muscularis mucosae are innervated by both type-I and type-II fibres. Type-I fibres also innervate pre-rete arteries. The rete- and gas-gland capillaries do not appear to be innervated. Arteries running to the resorptive area are innervated by type-I fibres. Both type-I and type-II profiles make contact with the muscularis mucosae in the resorptive area. Only type-I fibres innervate the radial dilator muscle in the oval sphincter region, whereas only type II fibres innervate the circular muscle of the oval sphincter. Type-I fibres took up α-methyl-noradrenaline, and could not be found after pre-treatment with 6-hydroxydopamine. They are, therefore, assumed to be adrenergic. Type-II fibres were tentatively identified, by exclusion, as cholinergic.
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  • 92
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    Cell & tissue research 237 (1984), S. 537-547 
    ISSN: 1432-0878
    Keywords: Scale ; Regeneration ; Ultrastructure ; Cichlid ; Hemichromis bimaculatus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Scale regeneration has been studied in Hemichromis bimaculatus. The removed scale, which serves as a control, is covered by its surrounding scleroblasts as can be seen with scanning electron microscopy. Subsequently, during regeneration, a population of scleroblasts arises in the empty dermal pocket as shown with transmission electron microscopy. At first, an elongated papilla of regeneration forms, probably from the differentiation of dermal fibroblasts. A scale anlage composed of the osseous layer appears in the middle of the papilla, which becomes a regenerating bag. All the surrounding large scleroblasts are involved in scale formation, although later three populations of scleroblasts specialize according to their location around the scale. Superficial scleroblasts flatten when the final thickness of the osseous layer of the scale is attained; the deep scleroblasts are responsible for the formation of the basal plate whereas marginal scleroblasts increase the diameter of the osseous layer of the scale. During scale regeneration, scleroblasts are more numerous and larger than during scale ontogenesis. In particular, deep scleroblasts form a columnar epithelium when the basal plate is laid down, a feature which is not found during scale ontogenesis. Moreover, the regenerated basal plate exhibits an orthogonal “plywood” arrangement that is never seen in the embryonic scale where the “plywood” is of the intermediate type.
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  • 93
    ISSN: 1432-0878
    Keywords: Epididymis (marsupials) ; Ultrastructure ; Sperm maturation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ductus epididymidis of the tammar is lined by an epithelium composed of principal, mitochondria-rich, apical and basal cells, and intraepithelial leucocytes. The epithelium is structurally differentiated into 6 zones referred to as the initial segment, middle segment (3 subdivisions) and terminal segment (2 subdivisions). The occurrence of the initial, middle and terminal segments corresponds quite closely to the anatomical differentiation of the epididymis into a head, body and tail. The initial segment epithelium in the tammar is lower and has shorter and more slender stereocilia than in other mammals which have been described. Otherwise, the structure of the epithelium has similar characteristics in the tammar to that described in other mammals. Spermatozoa begin to develop the capacity for motility within the initial segment, but only show structural signs of maturation in the middle segment. The sperm head rotates through 90 degrees in the proximal subdivision of the middle segment. The cytoplasmic droplet is detached and spermatozoa develop the capacity for motility in the middle subdivision of the middle segment. The cytoplasmic droplets are phagocytosed by the epididymal epithelium of the middle segment. Sperm storage appears to be the main function of the terminal segment.
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  • 94
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    Keywords: Peptidergic neurotransmission ; Lymnaea stagnalis ; Immunocytochemistry ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Three neuronal systems of the pond snail Lymnaea stagnalis were immunocytochemically investigated at the ultrastructural level with the unlabeled peroxidase-antiperoxidase technique. Preliminary electrophysiological and cell-filling investigations have shown that a cluster of neurons which reacts positively with an antiserum against the molluscan cardio-active peptide FMRFamide, sends axons to the penis retractor muscle. In this muscle anti-FMRF-amide (aFM) positive axons form neuro-muscular synapses with (smooth) muscle fibers. The morphological observations suggest the aFM immunoreactive system to be involved in peptidergic neurotransmission. In the right parietal ganglion a large neuron (LYAC) is penetrated by aFM positive axons which form synapse-like structures (SLS) with the LYAC. The assumption that the SLS represent the morphological basis for peptidergic transmission is sustained by the observation that iontophoretical application of synthetic FMRFamide depolarizes the LYAC. The axons of a group of pedal anti-vasopressin (aVP) positive cells run in close vicinity to the cerebral ovulation (neuro-)-hormone producing cell system (CDC system) Synapses or SLS between the two systems were not observed. The fact that (bath) application of arg-vasopressin induces bursting in the CDC, may indicate that the vasopressin-like substance of the aVP cells is released non-synaptically.
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  • 95
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    Keywords: Photoperiods ; Pituitary gland, pars tuberalis ; Ultrastructure ; Phodopus sungorus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Conspicuous cytological differences are found between specific secretory cells of the hypophysial pars tuberalis of Djungarian hamsters exposed to long and short photoperiods. The cells differ with respect to the shapes of perikarya and nuclei and show diverse amounts of secretory granules, lysosome-like bodies and glycogen.
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  • 96
    ISSN: 1432-0878
    Keywords: Pituitary gland, pars anterior (distalis) ; Gonadotrops ; Thyrotrops ; Ultrastructure ; Immunolabeling ; Teleosts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Pituitaries of the African catfish (Clarias lazera) were studied with immunocytochemical methods, at the light-microscopic and ultrastructural levels, for the characterization and localization of gonadotropic and thyrotropic cells. Two immunostaining procedures with the use of different markers were carried out: (i) with peroxidase-antiperoxidase, (ii) with protein A-gold. In routinely stained sections for light microscopy two types of basophils were identified in the proximal pars distalis: (1) large, round, purple cells, and (2) small, angular, light-blue cells. Both types were immunolabeled with antibodies against Clarias α,β-gonadotropin (GTH) and salmon G100-GTH. Only the large basophils were immunolabeled with anti-carp β-GTH, whereas the small basophils were the only cells immunolabeled with anti-human thyrotropin beta subunit (anti-h TSH-β). It was concluded that the large basophils represent the gonadotrops and the small basophils the thyrotrops. At the ultrastructural level the immunostaining of the GTH-cells was confined to three types of inclusions: (i) secretory vesicles, (ii) globules, and (iii) electron-dense, membrane-bound irregular masses. Especially the protein A- gold method, in combination with the use of a highly diluted homologous antiserum, resulted in a distinct localization of GTH. The presence of two types of nerve fibres, synaptically contacting the gonadotrops, is discussed with regard to the presence of a peptidergic (stimulatory) and an aminergic (inhibitory) control of GTH-secretion.
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  • 97
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    Cell & tissue research 238 (1984), S. 497-502 
    ISSN: 1432-0878
    Keywords: D2 glycoprotein ; Adrenal gland ; Immunocytochemistry ; Ultrastructure ; Cell adhesion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructural localization of the glycoprotein D2 in rat adrenal gland was investigated using immunohistochemical methods, and D2 localization in cultures of adult bovine chromaffin cells was studied by immunofluorescence. D2 was found to be situated on nerve fibers passing through the adrenal cortex and in the medulla zone, and also on the surface of all chromaffin cells. In addition, it was strongly expressed on the surface of glial (Schwann) cells. Cortical cells were unreactive to the antiserum. In cultures, all adrenalin and noradrenalin [dopamine-β-hydroxylase (DBH)-positive] cells were surface labelled for D2. A less frequent second cell type was recognized in vitro which was DBH negative but D2 positive. Such cells were presumed to be Schwann cells. These data are discussed in terms of the developmental origin of the cells and with regard to the putative functional rôle of D2 in cell adhesion phenomena.
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  • 98
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    Cell & tissue research 234 (1983), S. 411-425 
    ISSN: 1432-0878
    Keywords: Intestines (chicken) ; Innervation ; Catecholamines ; Adrenergic fibres ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Axons in the duodenum, ileum and rectum of the domestic fowl were identified as catecholamine-containing (CA) on the basis of positive reactivity following chromaffin fixation for electron microscopy. CA-axons in association with blood vessels in all regions of the intestine and in non-vascular sites in the small intestine had a ‘ typical’ adrenergic appearance, in that they contained many small granular vesicles (SGV) and variable numbers of large granular vesicles (LGV). In the rectum the non-vascular CA-axon profiles were atypical, in that there were many elongated LGV and few SGV, and the chromaffin reactivity was weak. The nerve profiles in the rectum were dramatically reduced following 6-hydroxydopamine and reserpine treatment and were absent in rectum cultured in the absence of extrinsic ganglia. It was concluded that the profiles, in spite of their low chromaffin reactivity, truely represent CA-axons. The possibility was raised that the atypical morphology and reduced chromaffin reactivity is due to the presence of adrenaline.
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  • 99
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    Cell & tissue research 234 (1983), S. 427-437 
    ISSN: 1432-0878
    Keywords: Corticotropes ; Rat fetus ; Ultrastructure ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Corticotropes of rat fetuses aged 16, 18 and 21 days were localized by the indirect antibody-enzyme method on semithin sections of the pituitary. The development of the ultrastructure of these cells was observed on consecutive ultrathin sections. In comparison with previous data our present results show that identification of a fetal cell type cannot be based entirely on morphological criteria. The structural peculiarities of corticotropes obtained from studies in vivo are compared with those observed in cells maintained in vitro.
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  • 100
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    Cell & tissue research 234 (1983), S. 679-689 
    ISSN: 1432-0878
    Keywords: Odontogenesis ; Rats ; Cyclophosphamide ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Cyclophosphamide-induced changes in rodent odontogenesis were investigated by light and electron microscopy in four-day-old Sprague Dawley rats given one injection of 40 mg/kg of body weight of cyclophosphamide and killed at intervals of one hour, one day, one week and two weeks. Incisor and molar teeth were dissected from the animals, fixed in 2.0% glutaraldehyde in 0.1 M sodium cacodylate with 3.4% sucrose, and subsequently some were incubated for alkaline phosphatase reaction, and embedded in Spurr's medium for sectioning at light- and electron-microscopic levels. From three days a cell-sparse zone was created in the pulp in the growing end of the tooth and progressive cellular changes were observed which became more severe in the one-week and two-week specimens. Subodontoblast and adjacent pulpal cells were the most affected showing nuclear changes, damage to, or loss of, organelles, and inclusion bodies. Odontogenic epithelium was less affected and odontoblasts appeared to be unaffected by the drug. A new irregular matrix was laid down in the defect area and seemed to be the product of depolarized odontoblasts. This new matrix showed alkaline phosphatase activity, as did the cells embedded in it, and later it became mineralized. It is speculated that the polarity of odontoblasts might be maintained by an intact subodontoblastic layer; when this is lost the odontoblasts become depolarized and capable of secreting matrix from both ends.
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