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  • Articles  (23)
  • Nodulation  (15)
  • Rice  (8)
  • Springer  (23)
  • 1980-1984  (23)
  • 1981  (23)
  • Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition  (23)
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  • Articles  (23)
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  • Springer  (23)
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  • 1980-1984  (23)
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  • Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition  (23)
  • 1
    ISSN: 1573-5036
    Keywords: Acetylen reduction assay ; Aluminium toxicity ; Chemical composition ; Cowpea ; Cultivar tolerance to soil acidity ; Liming ; Nitrogen deficiency ; Nitrogenase activity ; Nodulation ; Relative dry matter yields ; Soil acidity ; Ultisol ; Lime-induced yield depression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The differential response of six cowpea (Vigna unguiculata (L.) Walp) cultivars to liming was assessed in a greenhouse trial using an Ultisol (Typic Paleudult) from southeastern Nigeria. Lime rates from 0 to 5.0 t/ha were applied to give a range of soil pH, determined in 1∶1 soil/water from 4.25 to about 7.0. Without liming, relative dry matter yields of tops ranged between 46.6 and 76.8 percent. Significant yield responses to the first lime increment (0.5 t/ha) were observed particularly in the less tolerant cultivars and maximum yields were generally obtained with application of 1.6 to 2.5 t/ha lime. Among the six cultivars, TVu 4557 and Ife Brown were most tolerant, Vita-1 and Vita-3 were moderately tolerant, and TVu 1977-OD and TVu 4552 were least tolerant to soil acidity. The latter two cultivars were the most poorly nodulated when grown in the unlimed soil. The first lime increment enabled excellent nodulation to be achieved in all cultivars. Nitrogen analyses on tops confirmed the visual symptoms of nitrogen deficiency in two cultivars (Vita-1, TVu 1977-OD) grown in the unlimed soil. Analysis of data on chemical composition of plant tops and chemical soil characteristics gave indications that aluminium toxicity is the major growth limiting factor for cowpeas in this soil. This study, albeit with a limited range of cowpea germplasm clearly indicates that cowpea possesses considerable potential as a grain legume on strongly acid soils (pH〈5.0), even in circumstances where lime is not available. Low rates of lime (0.5 t/ha or less) will greatly expand the available choice of cultivars.
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  • 2
    ISSN: 1573-5036
    Keywords: Acetylene reduction assay ; Effect of N fertilizer ; N2 fixation ; Rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Unfertilized and NPK-fertilized plots in the long term fertility trial at Los Baños, Philippines were used to observe the effect of nitrogen fertilizer on heterotrophic N2-fixing activities in plow layer soil and in association with wetland rice. The activities were measured in the field byin situ acetylene reduction assays. Acetylene reduction activity of the plow layer soil in the paddy field was measured by soil cores. No appreciable difference was found-between fertilized and unfertilized plots.
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  • 3
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    Plant and soil 59 (1981), S. 415-425 
    ISSN: 1573-5036
    Keywords: Rice ; Zinc-phosphorus interaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A greenhouse experiment was conducted to study the effect of phosphorus and zinc application, in three lowland alluvial rice soils (Haplustalf) on the growth of rice and the concentration of phosphorus, zinc, copper, iron and manganese in shoots and roots. The results showed that application of phosphorus and zinc significantly increased the dry matter yield of shoots, grains and roots. Application of phosphorus caused a decrease in the concentration of zinc, copper, iron and manganese both in shoots and roots. Application of zinc also similarly lowered the concentration of phosphorus, copper and iron, but increased that of manganese in shoots and roots. The decrease in the concentration of the elements in the shoots was not due to dilution effect or to the reduced rate of translocation of the elements from the roots to tops. This has been attributed more to the changes in the availability of the elements in soil resulting from the application of phosphorus and zinc.
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  • 4
    ISSN: 1573-5036
    Keywords: Exopolysaccharide ; Lipopolysaccharide ; Neutral sugars ; Nodulation ; Plasmid ; Rhizobium trifolii
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The nodulatingRhizobium trifolii strain 24 and its non-nodulating mutant 24 nod−3 have been examined. The exopolysaccharides of both cultures studied contained mannose, galactose and glucose at similar molar ratios. On the other hand some quantitative differences have been found between the lipopolysaccharides in respect of the composition of neutral sugars. Glucose and rhamnose were the main constituents of the nodulating strain 24, whereas rhamnose and galactose in non-nodulating mutant 24 nod−3 deprived of the plasmid pWZ2.
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  • 5
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    Plant and soil 60 (1981), S. 205-211 
    ISSN: 1573-5036
    Keywords: Acetylene reduction ; Acid soil ; K application ; Liming ; N2-fixation ; Nodulation ; P application ; Rhizobium meliloti ; Root dry weight ; Shoot dry weight
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Alfalfa plants,Medicago sativa L., were selected from the Florida 66 cultivar for vigor in an acid (pH 4.4, Al≥.4 meq/100 g) and a limed, fertilized (pH 6.5, Al=0 meq/100 g, P and K added) Cecil topsoil. The selected plants were intermated by selection condition to achieve two germplasms, acid selected (A-1) and limed, fertile selected (L-1). ARhizobium meliloti strain (79-4s) was isolated from a high acetylene reducing nodule from a plant in a similar acid soil. The germplasms and the Rhizobium strain were then tested in greenhouse pots for agronomic performance under a variety of soil pH and fertility conditions. The 79-4s inoculum, as well as commercially prepared inoculum, gave better plant yield and acetylene reduction (N2-fixation) at all harvests when compared to a sterile peat control, but the commercial inoculum was the best inoculum treatment. The A-1 germplasm produced higher shoot dry weight at the final harvest than did the L-1 germplasm at all soil pH’s when P and K were applied at the highest rates. The A-1 germplasm also had better root weight (mainly fibrous roots) and acetylene reduction in these soil conditions. The two germplasms appear to be genetically distinct and respond differently depending on soil pH and fertility conditions.
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  • 6
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    Plant and soil 62 (1981), S. 183-192 
    ISSN: 1573-5036
    Keywords: Liming ; Nitrogen ; Rice ; Submerged rice ; Zinc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effect of N and Zn on the yield of submerged rice and uptake of N and Zn was studied on limed and unlimed soils in greenhouse. Nitrogen was applied at the rate of 0,80 and 160 ppm through urea, ammonium sulphate and ammonium nitrate, Zn at 0 and 10 ppm in limed (4% CaCO3) and unlimed soils. Liming (4% CaCO3) decreased dry matter yield of rice at tillering, heading and straw and grain at maturity significantly against unlimed treatments. Ammonium sulphate gave highest yield with all applied doses followed by ammonium nitrate and urea. Zinc at 10 ppm increased dry matter and grain yield significantly over no Zn treatment in rice. The N sources gave N concentration and uptake in rice plants in decreasing order: $$Ammonium sulphate 〉 Ammonium nitrate 〉 Urea.$$ The highest N concentration was recorded with 160 ppm followed by 80 ppm N through any source and lowest in control. The application of Zn increased and liming decreased N concentration and uptake by rice at all sampling stages. Highest N concentration (2.37 to 3.92%) was observed at tillering followed at heading (0.48 to 1.05%). At maturity N in grain ranged from 0.69 to 1.13% whereas in straw from 0.24 to 0.41%. There were positive significant interactions of N(S) x N(L), N(S) and N(L) x Zn and negative interaction with lime on yield, N concentration (%) and N uptake (mg/pot). The application of N sources and levels and Zn increased Zn uptake (μg/pot), and liming decreased it. But 160 ppm N through any source decreased straw Zn concentration over 80 ppm N in absence of added Zn, however, effects on grain were not so strong particularly that of 160 ppm, when 10 ppm Zn was also added even 160 ppm N increased Zn concentration over 80 ppm significantly in both straw and grain. In this case the effect of N sources on Zn concentration was in the order: $$Ammonium sulphate 〉 Urea 〉 Ammonium nitrate.$$
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  • 7
    ISSN: 1573-5036
    Keywords: Al ; Ca ; Lotus ; Mn ; N ; Nodulation ; P ; Pasture legumes ; Root morphology ; Soil pH ; White clover
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Effects of increasing rates of lime (0, 900, 1725, and 3000 kg Ca(OH)2/ha producing soil pH of 4.0, 4.7, 5.1 and 5.6) and P (50, 150, 250 and 350 kg P/ha) on top and root yield, root morphology and chemical composition of lotus (Lotus pedunculatus Cav.) and white clover (Trifolium repens L.), were studied, using an acid soil in a greenhouse experiment. Increasing rates of applied lime and phosphate resulted in substantial increases in top yields of both species but concomitant increases in root yield were small. In the unlimed soil, lotus out-yielded (tops and roots) white clover at all P levels. However, in the three limed treatments, white clover clearly out-yielded lotus. Yield response curves to applied P levelled off at the two highest lime rates for lotus but not for white clover. Nodulation and N content of white clover increased significantly with increasing lime applications, but for lotus there was a significant decrease in nodulation at the highest lime rate. Increased P rates had a small stimulatory effect on nodulation in both species. Of the total root weight, the percentage contribution of the tap and primary lateral root fractions was smaller and that of the secondary plus tertiary lateral roots was greater for lotus than for white clover although root length per unit weight tended to be larger for white clover at the two highest lime rates. Furthermore, lotus possessed longer and more numerous root hairs than white clover. Lime applications significantly decreased the percentage contribution of the tap and primary lateral roots to the total root weight and increased the percentage contribution of the secondary plus tertiary lateral roots. Al and Mn contents of tops and roots of both species decreased with increasing lime rates. There was a highly significant negative correlation between relative yield and Al content of lotus and white clover tops. In comparison with the limed treatments, in the unlimed treatments a greater percentage of total P, Al, Mn and N content accumulated in the roots of both species. In addition, lotus accumulated a much greater percentage Al in its roots than white clover.
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  • 8
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    Plant and soil 62 (1981), S. 305-308 
    ISSN: 1573-5036
    Keywords: Liming ; Nitrogen ; Phosphorus ; Rice ; Tryptophan ; Zinc
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effects of Zn, P, N and CaCO3 on tryptophan concentration in rice grain were studied in greenhouse at Haryana Agricultural University. Zinc application upto 20 ppm increased tryptophan concentration in rice grain. Zn-EDTA gave highest increase followed by ZnSO4 and then ZnO. Liming at the rate of 4 and 8 per cent decreased tryptophan concentration significantly. Phosphorus application upto 100 ppm also decreased tryptophan significantly but Zn in combination with P increased tryptophan and overcame negative effect of P. Nitrogen application upto 120 ppm increased tryptophan concentration. There was positive interaction between Zn and N. Ammonium sulphate gave highest tryptophan followed by ammonium nitrate and then urea. The tryptophan concentration ranged between 766 ppm and 2011 ppm in paddy grain. The lowest tryptophan concentration was in the plants treated with 8 per cent lime in absence of added Zn and highest with 10 ppm Zn through Zn-EDTA.
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  • 9
    ISSN: 1573-5036
    Keywords: Azotobacter ; Glycine max ; Nodulation ; Rhizobium ; Trifolium repens ; Vigna unguiculata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Azotobacter vinelandii strains caused the formation of increased numbers of root nodules onGlycine max, Vigna unguiculata andTrifolium repens by their respective rhizobial symbionts. Increased nodulation due to inoculation withA. vinelandii also occurred in field grownG. max. Mutant strains ofA. vinelandii unable to fix nitrogen caused nodulation increases comparable to those caused by nitrogen-fixing strains. This indicates that nitrogen fixation byA. vinelandii was not responsible for the enhanced nodulation. The effect ofA. vinelandii on nodulation was greatest when cells from the mid-exponential phase of growth were applied as inoculants. Non viable cell preparations ofAzotobacter vinelandii were also found to cause an increase in the number of root nodules formed onGlycine max Rhizobium japonicum under greenhouse conditions. The nodulation enhancement activity was influenced by the method chosen to kill theA. vinelandii cells. Heat treatment and treatment with lethal levels of streptomycin destroyed the activity, whereas the activity was unaffected by ultraviolet-light treatment of the cells. Cell-free extracts ofA. vinelandii were found to enhance nodulation. On the other hand, culture supernatants ofA. vinelandii had no effect on nodulation. A split-root experiment suggested that the agent(s) responsible for the increased nodulation was not translocatable throughout the plant. The results suggest a non-excretable protein, produced byA. vinelandii, as a possible mechanism for nodulation enhancement.
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  • 10
    ISSN: 1573-5036
    Keywords: Cations ; Interaction ; Mycorrhiza ; Nodulation ; Phosphate ; Trifolium subterraneum ; VAM
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Conclusions Mycorrhizal fungi increase the rate of phosphate uptake by roots (P inflow) over a range of soil P levels even when mycorrhizal growth increases no longer occur. It is likely that the fungi play a direct part in uptake and translocation of P to the roots. V.A.M. effects on nodulation and N2 fixation are largely indirect, probably resulting from improved P nutrition and growth at low soil P levels. Work on inorganic cation nutrition is much less advanced, but it is already clear that there are interactions between P nutrition and cation uptake which may also be indirect. The pattern of N assimilation (N2 fixation vis-à-vis NaNO3 or (NH4)2SO4 uptake) may modify cation/P interactions. Further work is required to distinguish cause and effect and to clarify the role played by V.A.M. fungi.
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  • 11
    ISSN: 1573-5036
    Keywords: Actinorhizas ; Ecology ; Gamma irradiation ; Hippophaë rhamnoides ; Longidorus ; Nematode ; Nodulation ; Root nodules ; Soil sterilization ; Succession
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary To explain the decline of Hippophaë scrub in the vegetation succession in the dunes of The Netherlands, the growth and nodulation of Hippophaë plants grown in pots, using soil from an early stage (site AH) and a post-optimum stage (site HP), were investigated. In HP-soil nodulation, yield, and the nitrogen and phosphorus content of test plants were always lower and the number of necrotic nodules and the dry matter content were always higher than in AH-soil, even after inoculation with crushed nodules and the addition of a nutrient solution. Plants in HP-soil also had darker roots, less root hairs, a higher number of short lateral roots and a higher percentage of dead roots than those in AH-soil. These characteristics of adverse growth conditions disappeared upon ignition or gamma-irradiation of HP-soil. Possible explanations of these results are discussed. The degeneration of Hippophaë scrub cannot be ascribed to the age of the plants, the absence of sufficient infective endophyte particles or to abiotic factors such as unfavourable physical (particle size) or chemical soil conditions but is caused by biotic factors. No indications were obtained that plant-pathogenic fungi and bacteria are involved. HP-soil in contrast to AH-soil, however, contained large numbers of the nematodeLongidorus sp., a species known to cause root deformations. The conclusion was that this nematode is one of the biotic factors involved in the degeneration of the Hippophaë scrub. This degeneration is due to a restriction of the root system resulting in a low phosphate uptake, a low nodulation capacity and, as a consequence, a low nitrogen content. The results demonstrate that biotic soil factors are important in influencing succession in higher plant communities.
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  • 12
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    Plant and soil 63 (1981), S. 465-476 
    ISSN: 1573-5036
    Keywords: Aphanothece ; Beijerinckia ; Klebsiella ; Mycobacterium ; N2-fixation ; Phyllosphere ; Rice ; Streptomyces
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The utility of spraying some known N2-fixing microorganisms on rice leaves grown both in N-less sand culture and under field conditions was examined. The effect was compared with that of spraying a phyllosphere N2-fixing isolate of Klebsiella, KUPBR2, and application of nitrogenous fertilizers. All the growth parameters studied including dry weight and N-content were enhanced. Under field conditions number of tillers was increased by 26% withKlebsiella pneumoniae M5al and by 65% with Aphanothece. The dry weight of the plants was enhanced by 61–119%. The yield per 10 m2 was almost doubled with Aphanothece, Beijerinckia 8007,Mycobacterium flavum, K. pneumoniae M5al and KUPBR2. The increases observed withStreptomyces sp. G12 though less spectacular was significant at 1% level with respect to several growth parameters.K. pneumoniae M5al,M. flavum andStreptomyces sp. G12 exhibited nitrogenase activity both in laboratory culture and in association with rice plants.
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  • 13
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    Plant and soil 59 (1981), S. 17-22 
    ISSN: 1573-5036
    Keywords: Drainage ; Drought tolerance ; Evapotranspiration ; Leaf-water potential ; Lysimeter ; Rice ; Root development ; Soil-moisture regime ; Transpiration ; Varietal response
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Effects of water table depths on plant-water status, and on growth and yields of two rice varieties (TOS 78 and TOS 848) were studied in a lysimetric investigation. A field study was also conducted on a hydromorphic soil to investigate the effects of fluctuating water table on rice. The leaf-water potential of TOS 78 monitored at 1300 hours at 50% flowering stage was −17.5 and −23.0 bars for 0 and 60 cm water table depths, respectively. When grown under soil moisture stress, TOS 848 maintained higher leaf-water potential (−17.5 barvs −22.5 bar) and yielded more than TOS 78. Under hydromorphic soil conditions, no-tillage treatments yielded the same as conventionally tilled plots.
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  • 14
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    Plant and soil 59 (1981), S. 207-215 
    ISSN: 1573-5036
    Keywords: Acetylene reduction ; Coffee plantation ; Inga jinicuil ; Nodulation ; Nutrients ; Woody legume
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effect of soil nutrients on nodulation byInga jinicuil, a leguminous tree used for shade in Mexican coffee plantations, is discussed. Nodulation and C2H2 reduction ofI. jinicuil seedlings, grown in soil, taken at different distances from coffee trunks, is described. Nodule biomass and activity are compared to the nutrient content of soils within various distances of coffee trunks. Seven coffee plantations, that employI. jinicuil for shade, were examined with respect to nodule biomass, C2H2 reducing activity, and soil characteristics. Highest nodule biomass was observed in soils with high levels of available phosphorus. High nitrogen content of the soil, on the other hand, appeared to be correlated with low nodule biomass. Potassium and magnesium, while apparently having little effect on nodule biomass, seem to be positively correlated with C2H2 reduction.
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  • 15
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    Plant and soil 59 (1981), S. 455-463 
    ISSN: 1573-5036
    Keywords: Acetylene reduction ; Acid soil ; Al uptake ; Liming ; N uptake ; Nodulation ; P application ; Plant persistence ; Root dry weight ; Seed germination ; Shoot dry weight
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effect of lime and P application on yield (top and root weigh), nodulation, intervally collected acetylene reduction (N2-fixation), and N and Al uptake of young alfalfa (46 days growth) were investigated in greenhouse pots containing acid Bladen or Bradson topsoils. The effect on seed germination and seedling persistence under these greenhouse conditions was also recorded. Alfalfa yield and acetylene reduction increased with lime and P additions in both soils, but, predominately, with P. There was no advantage of increasing these two parameters with liming past pH 6.0 provided P was adequate. Positive relationships (R2) existed between yield and acetylene reduction, and with both factors and root weight, nodule weight, and N uptake. Increased uptake of Al by alfalfa seedlings depressed yield, but data indicate P may block Al uptake at high soil pH. There were no treatment effects on seed germination, but P application increased plant persistence in the Bladen soil.
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  • 16
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    Plant and soil 60 (1981), S. 139-142 
    ISSN: 1573-5036
    Keywords: Legumes ; Nitrogen fixation ; Nodulation ; Winged beans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Only legumes of the cowpea cross-inoculation group, including the winged bean (Psophocarpus tetragonolobus) were found to form nodules in a temperate zone soil with no previous history of legume cropping. Isolates from root nodules from uninoculated winged beans grown in the field only nodulated legumes in the cowpea cross-inoculation group.Rhizobium japonicum formed ineffective nodules with the winged bean.
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  • 17
    ISSN: 1573-5036
    Keywords: Grain number ; Grain size ; Productivity ; Rate of grain growth ; Rice ; Spikelet filling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Studies on the nature of relationship between grain size (in terms of thousand grain weight), grain number, rate of filling and yield have been worked out with late duration (140–150 days) rice varieties. Grain number per m2, grains per panicle and yield showed a negative relationship with grain size. There was no compensatory balance between grain size and grain number within a range of thousand grain weight of 15 to 30 g. Based on the peaks in grain filling activity, the varieties were classified into four groups. There was no relationship between grain size and rate of grain filling thereby suggesting that grain filling is determined by inherent endogenous factors.
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  • 18
    ISSN: 1573-5036
    Keywords: Nutrient supply ; Nodule effectiveness ; Rhizobium competition ; Nodulation ; Lotus pedunculatus ; Lotus ; Rhizobium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effect of nutrient supply on nodule formation and competition between Rhizobium strains for nodulation ofLotus pedunculatus was studied. Limiting plant growth by decreasing the supply of nutrients in an otherwise nitrogen-free medium, increased the size but decreased the number and the nitrogenase activity of nodules formed by a fast-growing strain of Lotus Rhizobium (NZP2037). In contrast decreasing nutrient supply caused only a small decline in the size, number and nitrogenase activity of nodules formed by a slow-growing strain (CC814s). Providing small quantities of NH4NO3 (50 to 250 μg N) to plants grown with a normal supply of other nutrients stimulated nodule development by both Rhizobium strains and increased the nitrogenase activity of the NZP2037 nodules. Differences in the level of effectiveness (nitrogen-fixing ability) of nodules formed by different Rhizobium strains on plants grown with a normal supply of nutrients were less apparent when the plants were grown with decreased nutrient supply or when the plants were supplied with low levels of inorganic N. Inter-strain competition for nodulation ofL. pedunculatus between the highly effective slow-growing strain CC814s and 7 other fast- and slow-growing strains, showed CC814s to form 42 to 100% of the nodules in all associations. The greater nodulating competitiveness of strain CC814s prevailed despite changes in the nutrient supply to the host plant. A tendency was observed for partially effective Lotus Rhizobium strains to become more competitive in nodule formation when plant growth was supplemented with low levels of inorganic nitrogen.
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  • 19
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    Plant and soil 61 (1981), S. 93-111 
    ISSN: 1573-5036
    Keywords: Ineffective nodules ; Medicago laciniata ; Nitrogen fixation ; Nodulation ; Root temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Medicago laciniata, an annual leguminous plant of Saharo-Sindian origin, is particularly refractory to root nodulation by most strains ofRhizobium meliloti. Using a series of such bacterial strains belonging to the 8 groups of Brockwell and Hely, and a variety of environmental conditions, it was noted that several normally non-nodulating strains (at 20°C) produced ineffective nodules at root temperatures of 24°C to 28°C. Nodulation at 20°C failed to occur in the presence of a wide variety of test compounds and physical conditions. No phytoalexins or anti-Rhizobium growth inhibitors were isolated from inoculated root tissue at any temperature. Temperature shift experiments indicated no infection of the root hairs at 20°C, and infection threads produced at the permissive root temperature failed to elongate after transfer to 20°C. However, if meristematic activity had been initiated in the inner root-cortical cells as a result of infection thread penetration at 28°C, no blockage of nodule maturation occurred upon subsequent transfer to 20°C root temperature. Nodules produced at 28°C were completely devoid of nitrogenase activity, although the apical (but not the distal) regions contained normal-appearing bacteriods, surrounded by enclosing membranes, and possessed a fully functional leghaemoglobin. A shortage of metabolic energy did not appear to be involved in the ineffective response. A hypothesis to explain the nodulation phenomenon observed was based on the observation in the roots of 2 factors present at 20°C but not at 28°C.
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  • 20
    ISSN: 1573-5036
    Keywords: Boron toxicity ; Copper and zinc deficiencies ; Critical limits ; Dilute hydrochloric acid extraction ; Flooded soils ; Rice ; Soil tests
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Because zinc deficiency is a widespread disorder of wetland rice and copper deficiency may occur with it, 0.05M HCl was compared with 0.1M HCl, EDTA and DTPA as an extractant for available zinc and copper. It was also compared with the reflux method for boron assay. Thirty-three wetland rice soils were analyzed for zinc and copper by the four methods. Rice was grown on the flooded soils, scored visually for zinc deficiency, and the plants analyzed for zinc and copper content. In the boron study, 53 soils were extracted by the reflux method and the 0.05M HCl procedure. Rice was grown on the flooded soils, scored visually for boron toxicity, and the plants analyzed for boron content. Fourteen of the 16 soils on which rice showed zinc deficiency gave 〈1.0 mg/kg Zn by the 0.05M HCl method but values far in excess of the critical limits by the other methods. The r values for available and plant zinc were: 0.05M HCl (0.88**); 0.1M HCl (0.55**); EDTA (0.43**); and DTPA (0.31ns). Twelve of the zinc-deficient soils gave〈0.1 mg/kg Cu by the 0.05M HCl method but values exceeding the critical limits by the EDTA and DTPA methods. The r values for available and plant copper were: 0.05M HCl (0.74**), 0.1M HCl (0.64**), EDTA (0.28ns), and DTPA (0.20ns). The critical limit of 1.0 mg/kg by the 0.05M HCl extraction was confirmed for zinc deficiency and a tentative value of 0.1 mg/kg for copper deficiency proposed. The 0.05M HCl method separated boron-toxic soils from non-toxic soils and gave a better correlation (r=0.91**) between available and plant boron than the reflux extraction (r=0.84**). The toxic limit by the 0.05M HCl method was provisionally set at 4 mg/kg.
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  • 21
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    Plant and soil 62 (1981), S. 53-63 
    ISSN: 1573-5036
    Keywords: Acid soils ; Common beans ; Inoculation ; Liming ; Lime pelleting ; Nodulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effect of level of CaCO3, inoculation and lime pelleting on the nodulation, dry matter yield and % N content of common bean plants (Phaseolus vulgaris) grown in five acid soils was investigated in a greenhouse study. The soils represented a range in pH from 3.9 to 5.1, in exchangeable Al from 0.0 to 4 meq/100 gm, in exchangeable Mn from 0.35 to 2.32 me/100 gm, and in %C from 0.69 to 5.60. Nodule weight decreased with increasing %C and for the soil with highest %C (5.60) no nodules were observed. In soils with low organic matter and low exchangeable Al and Mn, inoculation increased nodule weight, dry matter yield and %N especially at the lowest pH level. Where the seeds were not inoculated, nodule weight and dry matter yield increased with soil pH. No such increases were observed where the seeds were inoculated. There was no apparent advantage in lime pelleting in such soils. In soils with low organic matter content and with substantial amounts of Al and/or Mn, liming increased nodule weight and dry matter yield, and decreased exchangeable Al and/or Mn. Lime pelleting was superior to mere inoculation in increasing nodule weight particularly at low lime rates. In soils with relatively high organic matter content, nodulation was very low or none at all. Low lime rates had little effect on exchangeable Al and Ca and dry matter yield. Higher lime rates, however, decreased exchangeable Al and dry matter yield but increased exchangeable Ca.
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  • 22
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 60 (1981), S. 349-356 
    ISSN: 1573-5036
    Keywords: Competition ; Cowpea ; Ineffective ; Mutagenesis ; Mutants ; Nodulation ; Rhizobium ; Symbiotic and asymbiotic nitrogen-fixation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A strain of cowpea Rhizobium was mutagenised and two ineffective mutants, M1 and M2, and an effective mutant, M3, were isolated. M1 produced more, but smaller nodules than the wild-type; these nodules lacked leghaemoglobin. M2 and the parental strain had similar nodulation characteristics, both forming large pink nodules. Plants inoculated with M3, nodulated earlier, produced more nodules (58%), had increased dry weights (26%) and the excised roots expressed greater acetylene (C2H2) reducing activity (39%) than plants inoculated with the wild-type. When competing with an indigenous population of effective rhizobia for nodule sites, M3 produced a higher proportion of the nodules (70–80%) than the parental strain (40–53%). M3 and the parental strain exhibited comparable rates of asymbiotic C2H2 reduction when grown on a defined medium, whereas M1 and M2 were inactive. The symbiotic properties of the mutants were unchanged after their reisolation following plant passage.
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  • 23
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 61 (1981), S. 189-202 
    ISSN: 1573-5036
    Keywords: Actinomycete symbiosis ; Alder ; Alnus glutinosa ; Endophyte ; Farmyard manure ; Forestry ; Frankia ; Inoculation ; Nitrogen fixation ; Nodulation ; Root nodules
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The occurrence and the infectivity of Frankia, the root-nodule endophyte ofAlnus glutinosa, were studied in different kinds of soil in the Netherlands. Both field and pot experiments indicated that many soils, on which alders have not been grown before, had low numbers of endogenous Frankia or none at all. Inoculation of these soils usually enhanced growth and nodulation of alders. The effect of fertilizer treatments on growth and nodulation ofA. glutinosa were studied in experimental plots. Alders grown in sandy soils, dressed with farmyard manure had the highest yield and the most nodules. The influence of inoculation with homogenates of Sp(+) and Sp(−) nodules and with a pure culture of Frankia AvcIl were studied in pot experiments. The quantity of different kinds of inoculum needed to obtain good growth and nodulation of alder was estimated. The results indicated that addition of a nodule homogenate of 90 g fresh AvcIl Sp(+) nodules is sufficient to inoculate one hectare of nursery soil to produce 10 nodules per plant, while a thousand times larger amount of inoculum is necessary when Sp(−) nodules are used. The limitations and the potentials of using nodule homogenates and pure cultures of Frankia for inoculation in forestry are discussed.
    Type of Medium: Electronic Resource
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