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  • 1
    Electronic Resource
    Electronic Resource
    Role of β-amylase in starch metabolism during soybean seed development and germination (1981)
    Oxford, UK : Blackwell Publishing Ltd
    Physiologia plantarum 53 (1981), S. 0 
    Details
    ISSN: 1399-3054
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Biology
    Notes: Differences in starch metabolism during seed development and germination of two soybean [Glycine max (L.) Merrill] genotypes with normal seed β-amylase activity [‘Williams’ (Sp1b and ‘Altona’ (Sp1b)] and two soybean genotypes with undetectable seed β-amylase activity [‘Chestnut’ (Sp1au) and ‘Altona’ (Sp1)] were investigated. Starch and soluble sugar profiles were essentially the same during seed development and germination. Total amylase activity of Williams and Altona (Sp1b) peaked just prior to seed maturity and then dropped off slowly; whereas, the total amylase activity of Chestnut and Altona (sp1) was very low throughout seed development and germination. The differences in amylase activity between Altona (Sp 1 b) and Altona (sp 1) was also seen in leaves. α-Amylase activity was similar in the four genotypes when β-amylase was inhibited with Hg2+ but was higher in the two genotypes with normal β-amylase activity when β-amylase was inhibited with heat plus Ca2+. Low levels of starch phosphorylase activity were detected throughout seed development and germination, and the activity was similar in three of the genotypes and higher in Altona (sp 1).The protein, oil and oligosaccharide contents of mature seeds of the four genotypes were similar. Altona (sp 1 b) and (sp 1), which appear to be near isogenic lines, were not different in any morphological character or yield.Altona (Sp 1 b) showed greater hydrolysis of soybean seed starch than Altona (sp 1), but the evidence indicates that the mutation resulting in greatly reduced or missing β-amylase activity has no effect on starch metabolism of developing and germinating soybean seeds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1399-3054.1981.tb02726.x
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  • 2
    Electronic Resource
    Electronic Resource
    Fatty Acid Alteration by a Δ9 Desaturase in Transgenic Tobacco Tissue (1992)
    [s.l.] : Nature Publishing Company
    Nature biotechnology 10 (1992), S. 675-678 
    Details
    ISSN: 1546-1696
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: [Auszug] Nicotiana tabacum tissue was transformed with a rat stearyl–CoA desaturase gene. Gas Chromatographie analysis showed an increase in monounsaturated 16 and 18 carbon fatty acids in selected transformed calli and leaves. Fractionation of lipid classes indicated that palmitoleic acid was found ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nbt0692-675
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  • 3
    Electronic Resource
    Electronic Resource
    Agrobacterium induced gall formation in lipoxygenase mutant isolines of soybeans (1991)
    Springer
    Plant cell reports 9 (1991), S. 651-654 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Agrobacterium-mediated transformation frequency is very low with cells from some species such as soybeans. Studies were conducted to investigate the Agrobacterium-mediated transformation frequency in near-isogenic lipoxygenase mutant lines of soybeans, since the nigh level of lipoxygenase activity in soybean embryos might be expected to affect interactions with Agrobacterium. The mutant line lacking lipoxygenase 3 showed significantly greater frequency of Agrobacterium-induced transformation than the other soybean lines. Stages of soybean embryo development which showed maximum differences in lipoxygenase 3 activity between mutant and wild-type, also showed maximum differences in transformation frequency. The increased transformation frequency with the absence of lipoxygenase 3 was only seen when both lipoxygenase 1 and 2 were present.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00231809
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  • 4
    Electronic Resource
    Electronic Resource
    Recovery of primary transformants of soybean (1989)
    Springer
    Plant cell reports 7 (1989), S. 615-617 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Three transformants of soybean, Glycine max (L.) Merr., have been recovered among a total of 18 plants regenerated by somatic embryogenesis from immature cotyledon tissues after cocultivation with Agrobacterium strains carrying a 15 kD zein gene (pH5PZ3D). DNA from upper leaves hybridized to a synthetic RNA probe specific for the zein sequence at a level equivalent to at least one copy per haploid genome. Hybridization to a vir G/C probe, however, was negligible, indicating that sequestration of whole bacteria or even persistence of plasmids within the tissues could not account for the zein hybridization signals. Progeny of all plants were uniformly untransformed. Since most somatic embryos have a multicellular origin in the regeneration system used, it is believed that the primary transformants were chimeric. The results indicate that somatic embryogenesis may be adaptable to Agrobacterium-mediated transformation in soybean, but that greater numbers of mitotic cycles under selection before embryo initiation will be required if somatic embryogenesis is to be used efficiently for production of plants with transformed germ-line cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00272042
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  • 5
    Electronic Resource
    Electronic Resource
    Analysis of lipid composition and morphological characteristics in soybean regenerants (1989)
    Springer
    Plant cell reports 7 (1989), S. 701-703 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A study was conducted to examine the extent of somaclonal variation of soybean plants, Glycine max (L.) Merrill cv. ‘McCall’, regenerated via somatic embryogenesis from cultured immature cotyledons using two different protocols. The sexual progeny of regenerants were compared with normal, seed-derived populations for morphological characteristics and fatty acid composition of seeds. First generation progeny of regenerants showed greater phenotypic variation than the control population, but this variation was not observed in the second generation. No stable somaclonal variants for fatty acid composition of the seed oil or morphological characteristics were observed, indicating that this somatic embryogenesis system should be adaptable for transformation with minimal generation of unwanted variation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00272065
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  • 6
    Electronic Resource
    Electronic Resource
    Agrobacterium induced gall formation in bell pepper (Capsicum annuum L.) and formation of shoot-like structures expressing introduced genes (1990)
    Springer
    Plant cell reports 9 (1990), S. 360-364 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The objective of this research was to define an in vitro regeneration and transformation system for bell pepper (Capsicum annuum L.) using six cultivars and one Guatemalan wild accession. The wild accession exhibited the best regeneration response. Only occasional elongation of shoot buds in ‘Yolo Wonder L’ was achieved by culture in the dark on a medium containing 10 mg/l BA and l mg/l IAA. Transformed shoot buds and leaf-like structures were obtained, showing beta- glucuronidase activity predominantly in the vascular and perivascular tissues, with no indication of contaminating Agrobacterium in the tissues. Attempts to regenerate whole transgenic plants from transformed shoot buds were unsuccessful.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232399
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  • 7
    Electronic Resource
    Electronic Resource
    Soybean somatic embryogenesis: interactions between sucrose and auxin (1988)
    Springer
    Plant cell reports 7 (1988), S. 517-520 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The interaction between sucrose and auxin in soybean (Glycine max L. Merr.) somatic embryogenesis was investigated by culturing immature cotyledon explants on factorial combinations of NAA (6.25, 12.5, 25 and 50 mg/l) and sucrose (0.5, 1, 2 and 4%). A significant interaction between sugar and auxin was observed; balanced concentrations of the two components were required for optimal embryo production and normality. The highest numbers of normal somatic embryos were produced on media which contained combinations of low to intermediate levels of sucrose (1 or 2%) and NAA (6.25 or 12.5 mg/l). Cotyledon explants from induction media having a low (0.5%) sucrose content showed the most efficient embryogenesis in secondary culture. The highest frequencies of germination (32 to 41%) were seen among embryos induced on media containing 0.5% sucrose.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00272745
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  • 8
    Electronic Resource
    Electronic Resource
    Effect of genotype on somatic embryogenesis from immature cotyledons of soybean (1989)
    Springer
    Plant cell, tissue and organ culture 16 (1989), S. 15-21 
    Details
    ISSN: 1573-5044
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Genotype has a large effect on the ability of immature soybean cotyledons to undergo auxin-stimulated somatic embryogenesis. Among 33 soybean lines, all those showing good regeneration were found to have in their pedigrees one or both of the highly regenerative ancestral lines, ‘Manchu’ or ‘A.K. Harrow’. When ‘Manchu’ was crossed with ‘Shiro’, a genotype showing extremely poor regeneration, F1 hybrid cotyledons showed intermediate regeneration capacity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00044068
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  • 9
    Electronic Resource
    Electronic Resource
    Cross-inoculation specificity ofPsophocarpus tetragonolobus (L.) DC (1981)
    Springer
    Plant and soil 60 (1981), S. 139-142 
    Details
    ISSN: 1573-5036
    Keywords: Legumes ; Nitrogen fixation ; Nodulation ; Winged beans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Only legumes of the cowpea cross-inoculation group, including the winged bean (Psophocarpus tetragonolobus) were found to form nodules in a temperate zone soil with no previous history of legume cropping. Isolates from root nodules from uninoculated winged beans grown in the field only nodulated legumes in the cowpea cross-inoculation group.Rhizobium japonicum formed ineffective nodules with the winged bean.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02377119
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  • 10
    Electronic Resource
    Electronic Resource
    Effects of some natural volatile compounds on the pathogenic fungiAlternaria alternata andBotrytis cinerea (1992)
    Springer
    Journal of chemical ecology 18 (1992), S. 1083-1091 
    Details
    ISSN: 1573-1561
    Keywords: Headspace ; aldehydes ; E-2-hexenal ; monoterpenes ; lipoxygenase ; spores ; hyphae ; wounding ; Lycopersicon esculentum ; Alternaria alternata ; Botrytis cinerea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A bioassay system was developed to test the effects of volatile compounds on the growth of hyphae from germinating fungal spores. Volatiles from crushed tomato leaves inhibited hyphal growth of two fungal pathogens,Alternaria alternata andBotrytis cinerea. Aldehydes, including C6 and C9 compounds formed by the lipoxygenase enzyme pathway upon wounding leaves, inhibited growth of both fungal species. Terpene hydrocarbons, 2-carene and limonene, had no significant effect on hyphal growth. The quantities of volatile compounds in the vapor phase of the bioassay system were measured by direct headspace sampling and GC analysis.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00980064
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