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  • Articles  (46)
  • Electron microscopy  (46)
  • Springer  (46)
  • American Chemical Society (ACS)
  • 1980-1984  (46)
  • 1980  (46)
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  • Articles  (46)
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  • 1980-1984  (46)
Year
Topic
  • 11
    Electronic Resource
    Electronic Resource
    Scanning and transmission electron microscopic observations of the cloacal epithelia of the domestic fowl (1980)
    Springer
    Cell & tissue research 211 (1980), S. 83-93 
    Details
    ISSN: 1432-0878
    Keywords: Epithelium ; Cloaca ; Electron microscopy ; Hen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The epithela of the three divisions (coprodaeum, urodaeum, proctodaeum) of the cloaca of the hen, and of the excretory ducts (colon, ureter, vagina) which join the divisions, are described using light microscopy, and scanning and transmission electron microscopy. Each region of the cloaca has its typical epithelium. Special attention is focussed in this study on the boundaries between the different epithelia. The coprodaeal epithelium does not differ considerably from that of the colon; a transitional zone is not visible. Distinct border zones, however, are observed between the other regions (ureter — urodaeum; vagina — urodaeum and proctodaeum; urodaeum-proctodaeum; proctodaeum — cutis). Although the vaginal opening is generally thought to lie in the urodaeum, our investigations show that at the vaginal opening into the cloaca the ciliated epithelium changes, on one border to a secretory epithelium characteristic of the urodaeum and on the other border to that characteristic of the proctodaeum. These observations are discussed in relation to functional aspects.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233725
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  • 12
    Electronic Resource
    Electronic Resource
    Ultrastructural alterations of the pancreatic D cell in the domestic fowl following vagotomy (1980)
    Springer
    Cell & tissue research 211 (1980), S. 171-174 
    Details
    ISSN: 1432-0878
    Keywords: Pancreatic D cell ; Neural control ; Vagotomy ; Electron microscopy ; Fowl
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In an attempt to determine the neural control of pancreatic D cells, the pancreatic islets of the domestic fowl were examined electron microscopically from 1 to 28 days after abdominal vagotomy. Exocytotic release of many secretory granules from D cells occurred one day after vagotomy. Rough endoplasmic reticulum developed and formed an arrangement of concentric whorls in the cytoplasm of D cells after axotomy. The altered D cells were also characterized by the occurrence of many peculiar dense bodies in the apical cytoplasm at all time periods studied. These bodies varied in shape and size, containing several round vesicles. The D cells were extensively depleted of granules after the longer time periods following vagotomy. The present results provide new morphological evidence for the vagus-nerve control of D cells, which may regulate the activity of islet cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233732
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  • 13
    Electronic Resource
    Electronic Resource
    Fetal hypothalamic transplants in the third ventricle of the adult rat brain (1980)
    Springer
    Cell & tissue research 211 (1980), S. 191-206 
    Details
    ISSN: 1432-0878
    Keywords: Hypothalamus ; Transplants ; Vasopressin ; Median eminence ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Blocks of anterior hypothalamus were transplanted from 19 day-old fetuses of Wistar/Lewis rats into the third ventricle of adult male Brattleboro rats. Physiological changes in graft recipients and in sham-operated animals were monitored daily. Twenty days after surgery, the graft recipients and shamoperated animals were killed and their brains examined by correlative scanning and transmission electron microscopy. Host animals that exhibited both decreased polydipsia and increased urine concentration were found to have viable grafts within the third ventricle. The observed physiological changes suggested that synthesis and release of vasopressin occurred in the transplanted neurons. Grafts were well vascularized by vessels arising from the host hypothalamus. Neurons, with perikarya ranging from 8 to 30 μm in diameter, glial cells, and neurites were located throughout the transplants. A neurohemal contact zone, similar to that normally seen in the median eminence, could not be demonstrated in the grafts. The absence of complete glial and ependymal barriers indicates a relatively close association between cells in the transplants and the cerebrospinal fluid. A large increase in supraependymal neurons and their processes, including an eruption of neurons through the floor of the third ventricle in one animal, was observed in graft recipients but not in shamoperated animals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00236442
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  • 14
    Electronic Resource
    Electronic Resource
    Fine structure of ependymal cysts in and around the area postrema of the rat (1980)
    Springer
    Cell & tissue research 206 (1980), S. 303-318 
    Details
    ISSN: 1432-0878
    Keywords: Area postrema, rat ; Ependyma ; Cyst ; Circumventricular organs ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Peculiar cells forming cysts were observed in the area postrema and sometimes also in the choroid plexus and the tela chorioidea near the area postrema, and were studied in detail by electron microscopy. The cytological features of the cyst cell and its junctional relationship to neighboring cells imply that cyst cells are derived from ependymal and choroid epithelial cells. The cyst cells usually contact directly the perivascular spaces of postremal, choroidal or pial capillaries, where the cytoplasm is often considerably attenuated. The cystic lumen is commonly filled with a flocculent material. The limiting membrane of the cystic lumen, which frequently bears cilia and microvilli, has the same thickness as the surface cell membrane. In many cases, the cyst is surrounded by the cytoplasm of a single cell. In some cases, however, two cells participate in the formation of the cyst, although one is only a slender process and joined by a zonula occludens with the main cyst cell. Horseradish peroxidase (HRP) injected into the cerebrospinal fluid (CSF) space failed to enter the cystic lumen. A possible significance of the cyst in relation to the CSF and blood circulation was considered.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232774
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  • 15
    Electronic Resource
    Electronic Resource
    Incorporation of 3H-oleic acid by the proximal convoluted tubule cells of the chick (Gallus domesticus) (1980)
    Springer
    Cell & tissue research 210 (1980), S. 447-459 
    Details
    ISSN: 1432-0878
    Keywords: Lipid ; Kidney tubules, proximal ; Autoradiography ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Lipid metabolism in the cells of the renal proximal convoluted tubules (PCT) was investigated in healthy fowls and in fowls with the Fatty Liver and Kidney Syndrome (FLKS). The tissue was fixed at 10–25 min intervals after intravenous injection of 3H-oleic acid. The distribution of autoradiographic grains was analysed by the “circle method”. In normal cells most of the silver grains were associated with the cytoplasmic organelles. Lipid droplets and Golgi elements had the highest specific activity relative to the nuclear activity, which was little above background level. Lysosome-like bodies and mitochondria had lower values. In the cells of the FLKS-affected birds a large proportion of the grains was located over the lipid droplets, which are abundant in this condition. The specific activity of the cytoplasmic organelles was barely 2-fold higher than the nuclear activity. The results suggest that there is a diminished incorporation of esterified fatty acids by the organelles of these cells and that the excess is transferred to the lipid droplets. The identity of low electron density particles observed in the PCT cells of severely affected birds is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00220201
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  • 16
    Electronic Resource
    Electronic Resource
    Gut hormones in Salamandra salamandra (1980)
    Springer
    Cell & tissue research 211 (1980), S. 331-343 
    Details
    ISSN: 1432-0878
    Keywords: Gut hormones ; Endocrine cells ; Electron microscopy ; Immunocytochemistry ; Peptidergic innervation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Histological, cytochemical and immunocytochemical methods were used in light and electron microscopical studies to demonstrate the presence of a neuroendocrine system in the gut of the urodele, Salamandra salamandra. Cytochemical stains capable of detecting peptide-producing endocrine cells demonstrate cells reacting with Masson's silver (argentaffin) method, Grimelius' argyrophil silver method, masked metachromasia method and the lead haematoxylin stain. Using antisera raised to a variety of mammalian gut peptides, cells containing bombesin-, gastrin-, somatostatin-, substance P- and glucagon-like immunoreactivity were identified; vasoactive intestinal polypeptide- and substance P-like immunoreactivities were found in nerve fibres in the submucous and myenteric plexus. No immunoreactivity was detected for motilin, gastric inhibitory polypeptide, cholecystokinin or secretin. The ultrastructure of the immunoreactive cells and nerves was revealed by the semithin/thin method. All the cells identified contained numerous electrondense secretory granules, which varied in their chracteristic morphological structure from one cell type to another. The evidence collected in this study indicates that a complex neuroendocrine system regulating gut function is present in this amphibian and may have developed prior to the emergence of the phylum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00236453
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  • 17
    Electronic Resource
    Electronic Resource
    Monoaminergic neurons of the mudpuppy retina (1980)
    Springer
    Cell & tissue research 210 (1980), S. 269-282 
    Details
    ISSN: 1432-0878
    Keywords: Monoaminergic neurons ; Retina ; Amacrine cells ; Neurotoxins ; Mudpuppy, Necturus maculosus ; Neurotransmitters ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The mudpuppy retina was investigated with the histofluorescence method of Falck and Hillarp in normal animals and in animals injected intraocularly with α-methylnoradrenaline, 5,6-dihydroxytryptamine, or a combination of the two drugs. Catecholaminergic amacrine cells were found to form a thin layer of terminals at the border between the inner nuclear and the inner plexiform layers. Catecholaminergic interplexiform cells were not found. Indoleamine-accumulating amacrine cells were also observed. They are fifteen to twenty times more numerous than the catecholaminergic cells, and their terminals occur diffusely throughout the inner plexiform layer. In a number of eyes the majority of the indoleamine-accumulating terminals were eliminated with intraocular injections of the neurotoxin, 5,7-dihydroxytryptamine, but the reproducibility of this effect was not consistent. Intravitreal injections of 5,6-dihydroxytryptamine were used to label both types of neurons for electron microscopy. They were found to make conventional type synapses on amacrine cells and, less frequently, on bipolar cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00237615
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  • 18
    Electronic Resource
    Electronic Resource
    Merkel cells and nerve endings in the labial epidermis of a lizard (1980)
    Springer
    Cell & tissue research 210 (1980), S. 353-357 
    Details
    ISSN: 1432-0878
    Keywords: Merkel cells (reptiles) ; Epidermis ; Lizard ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Examination of the labial epidermis of the lizard Lacerta sicula revealed cells displaying all features of Merkel cells. These cells are located in the stratum basale of epidermal pegs and are arranged in clusters.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00237623
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  • 19
    Electronic Resource
    Electronic Resource
    Ultrastructural changes in Paneth cells during hibernation in the ground squirrel Spermophilm lateralis (1980)
    Springer
    Cell & tissue research 211 (1980), S. 293-301 
    Details
    ISSN: 1432-0878
    Keywords: Paneth cell ; Hibernation ; Intestine ; Hypothermia ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure of Paneth cells from jejuno-ileal segments of the small intestine of the ground squirrel, S. lateralis, was examined under normal euthermic conditions and during the profoundly depressed metabolic conditions of natural hibernation. Paneth cells obtained from hibernating animals gave evidence of markedly reduced activity when compared to Paneth cells from euthermic animals. In hibernating animals, the nuclei were smaller, with less prominent nucleoli and with an increased proportion of heterochromatin. In hibernating animals, the rough endoplasmic reticulum was fragmentary and poorly organized, in contrast to the typical arrangement of concentric lamellae seen in euthermic animals. Although the total number of ribosomes was decreased in hibernating animals, there were proportionally more free ribosomes than in euthermic animals. Paneth cells from hibernating animals also contained a greater number of apical secretory granules which were smaller and more variable in electron density than granules from control animals. These ultrastructural features indicate that during hibernation the Paneth cell is relatively quiescent.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00236450
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  • 20
    Electronic Resource
    Electronic Resource
    Effect of testosterone on the female hamster harderian gland pigmentation and ultrastructure (1980)
    Springer
    Cell & tissue research 207 (1980), S. 511-517 
    Details
    ISSN: 1432-0878
    Keywords: Female hamster ; Harderian gland ; Testosterone ; Tubular clusters ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Distinct differences occur in the pigmentation and ultrastructural features of the Harderian glands in male and female hamsters. The results of a study on the effect of testosterone on the fine structure of the female Harderian glands are presented here. Glands from three groups of hamsters were examined at intervals up to 49 days: (1) testosterone injected, receiving 2mg testosterone propionate in 0.1 ml sesame oil per day; (2) sham-injected, receiving 0.1 ml sesame oil per day; (3) untreated controls. Testosterone injections caused a reduction in the number of dark-brown pigment granules in the acinar cells starting on the 6th day, whereas clusters of tubules, typical of adult male glands, appeared on the 4th day and increased in number thereafter. Lamellar structures, normally present in the female gland, decreased in testosterone treated specimens. These changes reversed after cessation of testosterone treatment. It is concluded that exogenous testosterone administered to female hamsters modifies the pigmentation and ultrastructure of their Harderian glands towards the male type and that this is a reversable phenomenon. There also appears to be an inverse relationship between the presence of tubular clusters in the acinar cells, and the degree of pigmentation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224624
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