ALBERT

All Library Books, journals and Electronic Records Telegrafenberg

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
Filter
  • Rat  (39)
  • Nicotiana  (26)
  • Wheat  (20)
  • nitrogen
  • phosphorus
  • Springer  (89)
  • Blackwell Publishing Ltd
  • National Academy of Sciences
  • 1990-1994
  • 1980-1984  (89)
  • 1925-1929
  • 1980  (89)
Collection
Keywords
Publisher
  • Springer  (89)
  • Blackwell Publishing Ltd
  • National Academy of Sciences
Years
  • 1990-1994
  • 1980-1984  (89)
  • 1925-1929
Year
  • 1
    Electronic Resource
    Electronic Resource
    Fertilizer regime and changes in the phosphate and potash status of a silty marine clay soil during 25 years (1980)
    Springer
    Nutrient cycling in agroecosystems 1 (1980), S. 95-101 
    Details
    ISSN: 1573-0867
    Keywords: soil fertility ; phosphorus ; potassium ; green manure ; crop rotation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Changes in the soil phosphorus and potassium status on three farming systems in the Northeast Polder during 25 years are related to the fertilizer regime over this period. The changes in the nutrient status of the soil are reflected in fertilizer policy. High rates of phosphate dressings markedly increased the phosphate status of the soil and built up a reserve of phosphate. In contrast, the potassium status decreased by about half, because only potatoes in the rotation were dressed with potassium. Methods of fertilization are discussed and suggestions are made with regard to possible improvements.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01073181
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Fine structure of fetal rat calvarium; provisional identification of preosteoclasts (1980)
    Springer
    Calcified tissue international 31 (1980), S. 21-28 
    Details
    ISSN: 1432-0827
    Keywords: Rat ; Calvarium ; Electron microscopy ; Preosteoclasts ; Osteoclasts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary This is a study of the fine structure of cells of the 20-day fetal rat calvarium. Special attention is given to identifying and characterizing preosteoclasts. These cells are relatively common and located largely, but not exclusively, at the endocranial bone surface. The preosteoclasts are characterized by abundant mitochondria, an incomplete perinuclear Golgi apparatus, and variable-shaped dense granules. The dense granules are unique in appearance in that they contain an internal dense matrix surrounded by a clear halo. Most granules are circular in shape but some are elongate or tubular in form. Granules with identical appearance are observed in osteoclasts. The preosteoclasts are mononucleate, or occasionally binucleate. It is suggested that because preosteoclasts are morphologically distinctive and relatively abundant, it should be feasible to separate these cells from a heterogeneous cell isolate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02407163
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    An automatic microspectrophotometric scanning method for the measurement of bone formation rates in vivo (1980)
    Springer
    Calcified tissue international 32 (1980), S. 63-68 
    Details
    ISSN: 1432-0827
    Keywords: Rat ; Bone formation ; Fluorochrome ; Microphotometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary A method for quantitative studies of the formation rate of bone has been developed. After vital staining with calcein, the fluorescence of a bone section was measured with a microphotometer controlled by a mini computer. After staining the bone structure with alizarin red S in a second step, the section was measured in transmitted light. The two data sets were combined and the shortest distances between the bone surface and the fluorescence lines were computed. With this information the distance distribution and the bone area between the label and the surface could be calculated in two different ways: with the single labeling and the continuous labeling techniques. The advantages and disadvantages of the two methods are discussed and compared with those of other techniques.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02408522
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    A quantitative histologic analysis of the growing long bone metaphysis (1980)
    Springer
    Calcified tissue international 32 (1980), S. 113-122 
    Details
    ISSN: 1432-0827
    Keywords: Rat ; Bone ; Metaphysis ; Quantitative ; Aging
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The purpose of this work was to analyze the proximal tibial metaphysis of the 170 g rat in a quantitative histologic fashion which would allow some relation to tissue age to be established. Stained 3 µm thick tissue sections were analyzed with the aid of a Merz grid on an eyepiece reticule and a light microscope. Tissue mass and cell distribution were studied in all areas. The rate of change in tissue mass during aging of the metaphysis was calculated. Two regions of the metaphysis were identified. One, corresponding to the primary spongiosa, less than 4.45 days of age, is a region of high turnover of hard tissue and high numbers of osteoblasts and osteoprogenitor cells. The other, corresponding to the secondary spongiosa, is a region of relatively low net tissue turnover and low numbers of osteoblasts and osteoprogenitor cells. Osteoclasts were found relatively more uniformly distributed through the metaphysis than were osteoblasts and osteoprogenitor cells. The rate of bone formation in the primary spongiosa is 50 times that found in the Haversian bone of the rib of 5-year-old humans and about 500 times that found at the cortical-endosteal surface of ribs of 5-year-old humans. It is argued that both cell distribution and tissue distribution in the metaphysis support the concept that osteoblasts and osteoclasts, rather than osteocytes, are responsible for the maturation of the metaphysis. The inhomogeneous distribution of both cells and tissue in the metaphysis has definite meaning for the interpretation of findings concerning the incorporation of radionuclides into the skeleton.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02408530
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Role of growth hormone in experimental phosphorus deprivation in the rat (1980)
    Springer
    Calcified tissue international 32 (1980), S. 105-112 
    Details
    ISSN: 1432-0827
    Keywords: Rat ; Hypophysectomy ; Dietary phosphorus deprivation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The demands of growth are known to exacerbate the effect of phosphorus deprivation (PD). We examined whether changes associated with PD could be prevented in young rats in which growth and growth hormone (GH) were eliminated by hypophysectomy (HPX) and whether PD in normal intact rats (INT) was associated with increased secretion of GH. INT or thyroxine- and ACTH-replaced HPX rats were fed one of the three diets: 0.31% P (NP); 0.027% P (LP), and 0.31% P, pair-fed with LP-mates (NP-PF). The results indicate that HPX did not qualitatively alter several physiologic responses to PD: (a) serum and urinary phosphorus (P) decreased and urinary calcium (Ca) increased; (b) net intestinal Ca retention fell and duodenal sac uptake of45Ca rose; and (c) external P balance was restored and duodenal sac uptake of32P-phosphate increased. Only the hypercalcemia seen in INT, LP rats was prevented by HPX. In INT rats serum immunoassayable GH levels, measured in single samples, were not different between different dietary groups while pituitary bioassayable GH was reduced in both LP and NP-PF rats when compared to the NP rats. Thus, except for hypercalcemia, the physiologic responses associated with PD are not prevented by the elimination of growth and GH, and the development of these responses in INT rats was not associated with a consistent or specific alteration in GH secretion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02408529
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Comparison of proteins synthesized in vivo and in vitro by mRNA from isolated protoplasts (1980)
    Springer
    Planta 148 (1980), S. 453-454 
    Details
    ISSN: 1432-2048
    Keywords: mRNA ; Protein synthesis ; Protoplasts ; Nicotiana ; Translation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Studies of proteins synthesized in vitro by messenger RNA (mRNA) extracted from tobacco protoplasts showed that the changes in protein synthesis and especially the lack of certain proteins observed previously in isolated protoplasts did not result from a failure of translation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02395314
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Influence of leaves and roots on meristem development inNicotiana tabacum L. cv. Wisconsin 38 (1980)
    Springer
    Planta 148 (1980), S. 462-467 
    Details
    ISSN: 1432-2048
    Keywords: Flower formation ; Leaves (in flower formation) ; Meristem (shoot) ; Nicotiana ; Roots (and flower formation)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The terminal, apical shoot meristem ofN. tabacum cv. Wisconsin 38 normally differentiates into a flower after producing 30 to 40 nodes. The influence of leaves and roots on the regulation of flowering was evaluated by counting the number of nodes produced after removal of leaves or the induction of adventitious roots. Leaf removal has no effect on the number of nodes produced before flower formation. Root induction significantly increases the number of nodes produced before flower formation. The plant behaves as if it were measuring the number of nodes between the meristem and the roots as a means of regulating meristem conversion from vegetative to floral differentiation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02395316
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Polysomes from expanded tobacco leaves (1980)
    Springer
    Planta 148 (1980), S. 491-497 
    Details
    ISSN: 1432-2048
    Keywords: Leaves (polysomes) ; Nicotiana ; Polysomes ; Poly(A)+ RNA ; Protein synthesis ; RNA (polysomal, polyA+)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The isolation of intact polysomes from leaves of tobacco (Nicotiana tabacum L.) is dependent on the age and state of development of leaves. Undegraded polysomes from young leaves in the early stages of expansion can be isolated easily by extracting the leaves in ice-cold extraction buffer (200 mM tris(hydroxymethyl)aminomethylmethane(Tris)-HCl, pH 9.0; 400 mM KCl; 200 mM sucrose; 35 mM MgCl2). Medium-size leaves give best yields of undegraded polysomes when extractions are carried out in the above buffer and in the presence of ethyleneglycol-bis-(β-amino-ethyl ether)-N,N′-tetracetic acid (EGTA) and mercaptoethanol. Isolation of polysomes from large, nearly fully expanded (mature) leaves requires all of the above plus diethyldithiocarbamate (DIECA) in the extraction medium. An extraction medium consisting of 25 mM EGTA, 0.01 M mercaptoethanol, 25 mM DIECA and 0.5% of the nonionic detergent, Nonidet-P40 (NP 40) was found to be very suitable for extraction of polysomes from all developmental stages of leaves. The polysomes extracted in the above medium showed active translation of protein in the wheat-germ in-vitro protein-synthesizing system. The translational products were similar when translations were carried out directly with polysomes or polysomal RNA, or polysomal poly(A)+ RNA from tobacco leaves. Poly(A)− polysomal RNA was a poor template in the in-vitro wheat-germ system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02395320
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    Nutritional requirements of protoplast-derived, haploid tobacco cells grown at low cell densities in liquid medium (1980)
    Springer
    Planta 149 (1980), S. 7-18 
    Details
    ISSN: 1432-2048
    Keywords: Auxin metabolism ; Cell suspensions (low density) ; Medium conditioning ; Nicotiana ; Nitrogen metabolism ; Protoplasts ; Vitamin requirements
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Preliminary attempts to define a completely synthetic medium able to support divisions of haploid tobacco mesophyll protoplasts at low initial densities have failed. High protoplast concentrations together with large amounts of naphtaleneacetic acid in the medium (3 mg l-1 NAA) were required for maximal induction of protoplast division. However, cell suspensions derived from haploid protoplasts after four days of preculture at high initial cell densities could be diluted to densities as low as 1–4 cells ml-1, provided the concentration of NAA in the medium was lowered to below 0.3 mg l-1. The optimal NAA supply for low cell density growth was affected by the nature of the nitrogen source. A simple minimal medium which supports the growth of these haploid cells with a plating efficiency of 30–40%, independent of the cell density in the range of 1–4 to 3·104 cells ml-1, has been established. In this medium inositol was the only vitamin stringently required for growth. Growth of cells at low densities was also possible in a medium initially containing 3 mg l-1 NAA, provided it was conditioned by the growth of protoplasts at high densities. Preliminary experiments with [14C]NAA showed that the amount of free NAA remaining in the medium after preincubation at high densities was drastically reduced. Simultaneously, NAA conjugates accumulated in the medium. The implications of these results are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00386221
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Characterization of a cytoplasmic auxin receptor from tobacco-pith callus (1980)
    Springer
    Planta 149 (1980), S. 44-47 
    Details
    ISSN: 1432-2048
    Keywords: Auxin receptor ; Callus ; Nicotiana
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cultured tobacco-pith tissue contains a cytoplasmic receptor for indoleacetic acid (IAA). The concentration of binding sites is very low in comparison to that of several auxin receptors found by other investigators. A few obvious possible causes (degradation or inactivation) were investigated. From the results we conclude that the low number of binding sites is real. The receptor binds IAA optimally at pH 7.5–7.8 and at a temperature of 24–30°C, when incubated for 25–30 min. The binding is very specific, as is shown by competition experiments. The concentration of the receptor in the callus tissue changes dramatically during each culture period, which suggests a possible role in development. The receptor was partly purified by gel filtration on Sepharose 6B followed by ion-exchange chromatography on DEAE-cellulose.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00386225
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 11
    Electronic Resource
    Electronic Resource
    The effect of light on the production of ethylene from 1-aminocyclopropane-1-carboxylic acid by leaves (1980)
    Springer
    Planta 149 (1980), S. 196-199 
    Details
    ISSN: 1432-2048
    Keywords: 1-Aminocyclopropane-1-carboxylic acid ; Avena ; Ethylene ; Etiolation ; Light (ethylene production) ; Nicotiana
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract White light inhibits the conversion of 1-amino-cyclopropane-1-carboxylic acid (ACC) in discs of green leaves of tobacco (Nicotiana tabacum L.) and segments of oat (Avena sativa L.) leaves by from 60 to 90%. Etiolated oat leaves do not show this effect. The general nature of the effect is shown by its presence in both a mono- and a dicotyledon. Since the leaves have been grown and pre-incubated in light, yet can produce from 2 to 9 times as much ethylene in the dark as in the light, it follows that the light inhibition is fully reversible. The inhibition by light is about equal to that exerted in the dark by CoCl2; it can be partly reversed by dithiothreitol and completely by mercaptoethanol. Thus the light is probably acting, via the photosynthetic system, on the SH group(s) of the enzyme system converting ACC to ethylene.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00380882
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 12
    Electronic Resource
    Electronic Resource
    Correlation between the nicotine content of tobacco plants and callus cultures (1980)
    Springer
    Planta 149 (1980), S. 205-206 
    Details
    ISSN: 1432-2048
    Keywords: Callus cultures ; Nicotiana ; Nicotine ; Tissue cultures
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Callus cultures of two low-alkaloid lines of Nicotiana tabacum L. had considerably lower nicotine contents than cultures from the respective highalkaloid cultivars which were isogenic except for the two loci for alkaloid accumulation. Thus, there was a strong correlation between the nicotine content of callus cultures and the plants from which they were derived.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00380884
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 13
    Electronic Resource
    Electronic Resource
    Auxin-binding by particulate fractions from tobacco leaf protoplasts (1980)
    Springer
    Planta 150 (1980), S. 9-12 
    Details
    ISSN: 1432-2048
    Keywords: Auxin binding ; Nicotiana ; Plasma membrane ; Protoplast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In vitro binding of 1-naphthaleneacetic acid (NAA) to particulate fractions from tobacco leaf protoplasts was studied. In freshly isolated protoplasts no specific binding could be detected, whereas it was present in particulate fractions from tobacco leaves. It is concluded that the NAA-binding-sites are probably located at the external face of the plasma membrane; they are destroyed during protoplast isolation by proteolytic enzymes in the cellulase and macerozyme preparations. After culturing the protoplasts for 3–4 d, the first cell divisions were observed and at the same time specific NAA-binding became detectable. The affinity constant for NAA was approx. 2·106 mol-1 and the number of binding sites increased during further culture.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00385607
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 14
    Electronic Resource
    Electronic Resource
    Factors influencing the incidence of habituation for cytokinin of tobacco pith tissue in culture (1980)
    Springer
    Planta 150 (1980), S. 264-268 
    Details
    ISSN: 1432-2048
    Keywords: Cytokinin habituation ; Habituation, cytokinin ; Nicotiana ; Position effects (habituation) ; Size effects (habituation) ; Tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Pith tissue of Nicotiana tabacum L. cv. “Havana 425” exhibits a gradient in its tendency to habituate for cytokinin on an auxin-containing medium at 35° C, about 10° C above the standard culture temperature. Explants of pith from below the 8th to 11th internode, counting from the bottom of the plant, rarely habituate for cytokinin; explants from above this threshold habituate rapidly. The explants must also be above a critical size, about 20–30 mg, to habituate. There was a pronounced interaction between size and position effects; the threshold position for cytokinin habituation shifted upward with decreasing explant size.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00390836
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 15
    Electronic Resource
    Electronic Resource
    Stimulation by abscisic acid of RNA synthesis in discs from healthy and tobacco mosaic virus-infected tobacco leaves (1980)
    Springer
    Planta 150 (1980), S. 349-353 
    Details
    ISSN: 1432-2048
    Keywords: Abscisic acid ; Nicotiana ; Ribonucleic acid synthesis ; Tobacco mosaic virus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Uptake of abscisic acid from the culture medium by discs of healthy and tobacco mosaic virus-infected tobacco leaves was measured. Small (two to five-fold) increases in abscisic acid concentration in discs caused increases in rates of [3H]uridine and [3H]adenine incorporation into total nucleic acid, virus RNA and host ribosomal RNA. Net accumulation of virus RNA was also enhanced by abscisic acid. This evidence for stimulation of RNA synthesis is compared with previous reports showing inhibition of RNA synthesis in other tissues. It is suggested that the increase in endogenous abscisic acid caused by tobacco mosaic virus infection may be at least partly responsible for observed increases in rates of RNA synthesis after infection.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00390168
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 16
    Electronic Resource
    Electronic Resource
    The role of peroxidase isoenzyme groups of Nicotiana tabacum in hydrogen peroxide formation (1980)
    Springer
    Planta 147 (1980), S. 467-470 
    Details
    ISSN: 1432-2048
    Keywords: Hydrogenperoxide ; Lignification ; Nicotiana ; Peroxidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Three peroxidase isoenzyme-groups found in cell walls of tobacco were tested for their capacity to form H2O2. Isoenzyme-group GI, located only in cell walls (GII and GIII are also found in protoplasts) showed the highest Kapp-value for H2O2-formation. The lowest Kapp-value, i.e., maximal H2O2-formation was received for group GIII which is ionically bound to the cell wall. As shown before, GI yields maximal polymerization rates for coniferyl- and p-coumarylalcohol. These facts indicate that each of the peroxidase isoenzyme groups of the cell wall is involved with different catalytic functions within the same pathways of H2O2-formation and succeeding lignification. H2O2-formation catalyzed by all 3 groups was increased by very low concentrations of Mn2+-ions. The required amount of Mn2+ leading to maximal stimulation was in each case dependent on the basic rate of H2O2-formation. Maximal stimulation of H2O2-formation by phenolic compounds was achieved by coniferylalcohol at a concentration of 10-4M for all groups. Stimulation by p-coumaryl-and by sinapylalcohol was not as significant.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00380189
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 17
    Electronic Resource
    Electronic Resource
    The induction of cytokinin habituation in primary pith explants of tobacco (1980)
    Springer
    Planta 149 (1980), S. 402-407 
    Details
    ISSN: 1432-2048
    Keywords: Cytokinin ; Habituation rates ; Nicotiana ; Temperature and habituation ; Tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Pith parenchyma tissue ofNicotiana tabacum L. cv. “Havana 425” becomes cytokinin habituated when incubated at 35°C on an auxin-containing medium. Under these conditions, habituated, hyperplastic nodules appear on the tissues. We used these nodules to estimate the incidence of habituation by a statistical method. The rate of habituation varied with the season. Tissue isolated from plants in the spring habituated approx. 7 times faster than did tissue isolated from plants in winter. The fact that the average rate, 〉4×10−3 per cell generation, was 100–1,000 times faster than the rate of somatic mutation inNicotiana species and depended on the physiological state of the tissue provides further evidence that habituation involves epigenetic changes rather than rare, random genetic mutations. We also found that kinetin (6-furfurylaminopurine) induced habituation and that the concentration required depended on the duration of cytokinin treatment. For long incubation times, approx. 6×10−10 M kinetin, which is about 1,000-fold lower than the concentration optimal for growth of cytokinin-requiring pith tissue, was sufficient to induce habituation. These results support the hypothesis that the habituated state is maintained by a positive feedback loop in which cytokinins either induce their own synthesis or inhibit their own degradation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00571176
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 18
    Electronic Resource
    Electronic Resource
    Fatty-acid composition and biosynthesis in cell suspension cultures of Glycine max (L.) Merr., Catharanthus roseus G. Don and Nicotiana tabacum L. (1980)
    Springer
    Planta 147 (1980), S. 384-388 
    Details
    ISSN: 1432-2048
    Keywords: Acetate incorporation ; Catharanthus ; Cell suspension cultures ; Fatty acids ; Glycihe ; Nicotiana ; Temperature and fatty-acid synthesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The fatty-acid composition of C. roseus and N. tabacum cell suspension cultures was unaffected by subculture on Wood and Braun, Murashige and Skoog, or Gamborg B5C media. However, placing the cultures — which were normally grown at 25° C — at 15° C reduced growth but resulted in enhanced formation of oleic and linolenic acids in C. roseus cultures and increased levels of linoleic and linolenic acids in cultures of G. max and N. tabacum, respectively. The incorporation of [14C]acetate into [14C]linoleic acid was more rapid in N. tabacum cells than in G. max cells, but was very poor in C. roseus where the [14C] label was distributed mainly between palmitic and oleic acids.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00380177
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 19
    Electronic Resource
    Electronic Resource
    Expression of nuclear and chloroplastic genes coding for fraction-1 protein in somatic hybrids of Nicotiana tabacum + rustica (1980)
    Springer
    Planta 147 (1980), S. 414-417 
    Details
    ISSN: 1432-2048
    Keywords: Fraction-1 protein ; Hybrid, somatic ; Nicotiana ; Protein (Fraction 1) ; Ribulose-1,5-bisphosphate carboxylase ; Somatic hybrid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In the sexual interspecific cross, Nicotiana rustica L.xN. tabacum L., N. rustica can serve as the female but not as the male parent. By fusion of protoplasts, the barrier to fertilization was overcome and somatic hybrids containing N. tabacum cytoplasm were produced as shown by isoelectric focusing of the Fraction-1 protein (F-1-protein). All somatic hybrids displayed polypeptides of the large subunit of F-1 protein (which is coded by the chloroplast genome) characteristic of only one or the other parental species. Two hybrids had large subunits of the N. tabacum type and two hybrids had those of the N. rustica type. Three hybrids contained three smallsubunit polypeptides (coded by the nuclear genome), one being characteristic of N. rustica, one characteristic of N. tabacum, and one with an isoelectric point common to both species. A fourth hybrid contained only two small-subunit polypeptides of the N. tabacum type but in a F-1 protein macromolecule whose large subunits were of the N. rustica type. One somatic hybrid was self-fertile and its F2 progeny contained large- and small-subunit polypeptides indistinguishable in their isoelectric points from those in the parent F1 hybrid. All somatic hybrids showed an aneuploid chromosome number and morphological characteristics intermediate between those of N. rustica and N. tabacum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00380181
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 20
    Electronic Resource
    Electronic Resource
    The effect of different temperatures on fatty-acid synthesis and polyunsaturation in cell suspension cultures (1980)
    Springer
    Planta 147 (1980), S. 389-395 
    Details
    ISSN: 1432-2048
    Keywords: Catharanthus ; Cell suspension cultures ; Glycine ; Fatty acids (synthesis, desaturation) ; Nicotiana ; Linoleic, linolenic, oleic, acids ; Temperature and fatty acids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cell suspension cultures of Catharanthus roseus G. Don, Glycine max (L.) Merr. and Nicotiana tabacum L. were incubated with [14C]acetate, [14C]oleic acid and [14C]linoleic acid at five different temperatures ranging from 15 to 35° C. When the incubation temperature was increased, [14C]acetate was incorporated preferentially into [14C]palmitate, with a concomitant drop in [14C]oleate formation. Between 15 and 20° C, [14C]oleic acid accumulated in C. roseus cells. In all cultures, optimum desaturation of [14C]oleic acid to [14C]linoleic acid occurred between 20 and 25° C, and in G. max this was also the optimal range for desaturation of [14C]linoleic acid to [14C]linolenic acid. Elongation of [14C]palmitic acid was inhibited when cultures grown at 15° C for 25 h were subsequently incubated with [14C]acetate at 25° C. [14C]oleic acid accumulated in G. max and C. roseus cultures grown at 35° C for 25 h and subsequently incubated at 25° C. Desaturation of [14C]oleic acid increased up to 25° C, but then decreased or leveled off depending on the cell line and on the temperature prior to incubation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00380178
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 21
    Electronic Resource
    Electronic Resource
    Polysomes from expanded tobacco leaves (1980)
    Springer
    Planta 148 (1980), S. 491-497 
    Details
    ISSN: 1432-2048
    Keywords: Leaves (polysomes) ; Nicotiana ; Polysomes ; Poly(A)+ RNA ; Protein synthesis ; RNA (polysomal, polyA+)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The isolation of intact polysomes from leaves of tobacco (Nicotiana tabacum L.) is dependent on the age and state of development of leaves. Undegraded polysomes from young leaves in the early stages of expansion can be isolated easily by extracting the leaves in ice-cold extraction buffer (200 mM tris(hydroxymethyl)aminomethylmethane(Tris)-HCl, pH 9.0; 400 mM KCl; 200 mM sucrose; 35 mM MgCl2). Medium-size leaves give best yields of undegraded polysomes when extractions are carried out in the above buffer and in the presence of ethyleneglycol-bis-(β-amino-ethyl ether)-N,N′-tetracetic acid (EGTA) and mercaptoethanol. Isolation of polysomes from large, nearly fully expanded (mature) leaves requires all of the above plus diethyldithiocarbamate (DIECA) in the extraction medium. An extraction medium consisting of 25 mM EGTA, 0.01 M mercaptoethanol, 25 mM DIECA and 0.5% of the nonionic detergent, Nonidet-P40 (NP 40) was found to be very suitable for extraction of polysomes from all developmental stages of leaves. The polysomes extracted in the above medium showed active translation of protein in the wheat-germ in-vitro protein-synthesizing system. The translational products were similar when translations were carried out directly with polysomes or polysomal RNA, or polysomal poly(A)+ RNA from tobacco leaves. Poly(A)− polysomal RNA was a poor template in the in-vitro wheat-germ system.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00552665
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 22
    Electronic Resource
    Electronic Resource
    Suitable conditions for characterization, identification, and isolation of the mRNA of the small subunit of ribulose-1,5-bisphosphate carboxylase from Nicotiana sylvestris (1980)
    Springer
    Planta 148 (1980), S. 211-216 
    Details
    ISSN: 1432-2048
    Keywords: mRNA ; Nicotiana ; Protein synthesis ; Ribulose-1,5-bisphosphate carboxylase ; RNA (messenger) ; Translation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The products synthesized in vitro by messenger RNA (mRNA) extracted from Nicotiana sylvestris were analyzed by electrophoresis on polyacrylamide slab gels. Only three of the major polypeptides synthesized are considered here: P55, P32, and P20. P55 and P32 were translated from chloroplast mRNA. P55 corresponds to the large subunit of ribulose-1,5-bisphosphate (RuP2) carboxylase; P32 is probably a chloroplast membrane protein. P20, the polypeptide synthesized from cytoplasmic poly(A)+ RNA, is the precursor of the small subunit of RuP2 carboxylase. The balance between P20 and P32, in which their relative proportions varied inversely, was regulated by the age of the leaves and the time of illumination; we took advantage of this phenomenon to isolate the mRNA from the small subunit in relatively large amounts. This mRNA has a molecular weight of 350,000.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00380029
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 23
    Electronic Resource
    Electronic Resource
    Properties and regulation of adenosine 5′-phosphosulfate sulfotransferase from suspension cultures ofNicotiana sylvestris (1980)
    Springer
    Planta 150 (1980), S. 140-143 
    Details
    ISSN: 1432-2048
    Keywords: Adenosine 5′-phosphosulfate sulfotransferase ; Cell suspension culture ; Cysteine ; Enzyme regulation ; Nicotiana
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The properties and the regulation of adenosine 5′-phosphosulfate sulfotransferase extracted from cell suspension cultures ofNicotiana sylvestris was investigated. Optimal adenosine 5′-phosphosulfate sulfotransferase activity was obtained from the cells by extraction with 0.1 M tris-HCl, pH8.0, containing 2 M MgSO4 and 10 mM dithioerythritol. The K m for adenosine 5′-phosphosulfate in the sulfotransferase reaction was about 11 μM. Adenosine 5′-phosphosulfate in concentrations above 50 μM were inhibitory. The extratable adenosine 5′-phosphosulfate sulfotransferase activity decreased during cultivation with sulfate as the sole sulfur source, but after about 3 days it reached a constant level (50 to 100 nmol activated sulfate transferred h-1 mg-1 protein) which was maintained for at least 24 h. Addition of 0.5 mM cysteine to the culture medium decreased the extractable adenosine 5′-phosphosulfate sulfotransferase activity and blocked growth completely. With 0.1 mM cysteine an enzyme level of about 10% of the initial value was reached within 6 to 12 h without significant inhibition of growth. The added cysteine was absorbed rapidly and after 24 h cysteine could no longer be detected in the medium. Before the cysteine was completely depleted, the activity of adenosine 5′-phosphosulfate sulfotransferase started to increase, reaching ultimately a level which was comparable to the initial value.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00582357
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 24
    Electronic Resource
    Electronic Resource
    Ribosylzeatin and zeatin in tobacco crown gall tissue (1980)
    Springer
    Planta 147 (1980), S. 269-273 
    Details
    ISSN: 1432-2048
    Keywords: Crown gall ; Cytokinins ; Nicotiana
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cytokinins were extracted from two cultures of tobacco crown gall tumor tissue: an unorganized tissue and a teratoma which produced leafy shoots. On Sephadex LH-20 column chromatography, extracts of both types of tissue yielded two peaks of cytokinin activity with elution volumes similar to ribosylzeatin and zeatin. Ribosylzeatin and zeatin were detected and quantified by coupled gas chromatography — mass spectrometry selected ion monitoring (GC/MS SIM), comparable quantities being found in the two extracts. Full mass spectral evidence for the presence of ribosylzeatin in both tissues was obtained. No evidence was found for the presence of N6-(Δ2-isopentenyl)adenosine (i6Ade) or N6-(Δ2-isopentenyl)adenine (i6Ade) although these compounds have been reported to occur in cytokinin-habituated tobacco callus tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00379831
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 25
    Electronic Resource
    Electronic Resource
    Multistage disruption of protoplasts by dikegulac (1980)
    Springer
    Planta 147 (1980), S. 274-276 
    Details
    ISSN: 1432-2048
    Keywords: Cell division ; Dikegulac ; Lysis ; Nicotiana ; Plasmalemma ; Protoplasts ; Tonoplast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Dikegulac (2,3:4,6 di-o-isopropylidine-2-keto-I-gulonate) is a growth regulator used to differentially kill terminal apices, and it analogously inhibits basic metabolic functions in dividing cells, but not stationary cells, in suspension culture. This report demonstrates an analogous situation in isolated tobacco protoplasts. At the lowest concentrations, dikegulac partially suppresses division of the protoplasts. Higher concentrations are required to produce visual cytoplasmic damage to the protoplasts, which probably first occurs at the level of the plasmalemma, as the vacuoles can be released intact. Later, tonoplast disruption occurs.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00379832
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 26
    Electronic Resource
    Electronic Resource
    Isolation of abscisic acid-resistant variants from tobacco cell cultures (1980)
    Springer
    Planta 148 (1980), S. 97-102 
    Details
    ISSN: 1432-2048
    Keywords: Abscisic acid ; Cell cultures ; Nicotiana ; Variants, resistant ; Water stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The goal of this work was to begin a genetic study of the molecular mode of action of abscisic acid (ABA), by isolating variant cultured cells resistant to the hormone, or to a factor which induces ABA synthesis, namely water stress. Cell cultures of Nicotiana tabacum L. cv. Wisconsin 38 and N. silvestris Speg. and Comes were chosen as the experimental materials. Studies of the effects of the two stresses on the growth of the cultures demonstrated that ABA or water stress imposed by mannitol could completely inhibit growth. These effects arose in both cases from a constant reduction of the growth rate of the cells throughout the culture period. Mannitol also induced an increase in ABA content of the cells and media of suspension cultures, although not to the concentrations required to achieve the same degree of growth inhibition when the hormone was applied exogenously.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00386408
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 27
    Electronic Resource
    Electronic Resource
    Isolation of abscisic acid-resistant variants from tobacco cell cultures (1980)
    Springer
    Planta 148 (1980), S. 103-107 
    Details
    ISSN: 1432-2048
    Keywords: Abscisic acid ; Cell cultures ; Nicotiana ; Variants, resistant ; Water stress
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Variant clones were isolated from Nicotiana silvestris Speg. et Comes cell cultures at low frequencies following severe abscisic-acid (ABA) or mannitol-induced water-stress treatments of plated cells. N. tabacum L. variants were not recovered. Variants from the ABA selection experiments exhibited a 10-fold increase in resistance to the hormone. This trait was stable in non-selective conditions for as long as was tested (200 days), but did not alter the response of the cells to water stress. Cell lines from the waterstress selection were not more resistant to mannitol than the parent line, and had a wide range of response to ABA.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00386409
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 28
    Electronic Resource
    Electronic Resource
    Comparison of proteins synthesized in vivo and in vitro by mRNA from isolated protoplasts (1980)
    Springer
    Planta 148 (1980), S. 453-454 
    Details
    ISSN: 1432-2048
    Keywords: mRNA ; Protein synthesis ; Protoplasts ; Nicotiana ; Translation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Studies of proteins synthesized in vitro by messenger RNA (mRNA) extracted from tobacco protoplasts showed that the changes in protein synthesis and especially the lack of certain proteins observed previously in isolated protoplasts did not result from a failure of translation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00552659
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 29
    Electronic Resource
    Electronic Resource
    Influence of leaves and roots on meristem development inNicotiana tabacum L. cv. Wisconsin 38 (1980)
    Springer
    Planta 148 (1980), S. 462-467 
    Details
    ISSN: 1432-2048
    Keywords: Flower formation ; Leaves (in flower formation) ; Meristem (shoot) ; Nicotiana ; Roots (and flower formation)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The terminal, apical shoot meristem ofN. tabacum cv. Wisconsin 38 normally differentiates into a flower after producing 30 to 40 nodes. The influence of leaves and roots on the regulation of flowering was evaluated by counting the number of nodes produced after removal of leaves or the induction of adventitious roots. Leaf removal has no effect on the number of nodes produced before flower formation. Root induction significantly increases the number of nodes produced before flower formation. The plant behaves as if it were measuring the number of nodes between the meristem and the roots as a means of regulating meristem conversion from vegetative to floral differentiation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00552661
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 30
    Electronic Resource
    Electronic Resource
    Glutenin subunits of genetically related European hexaploid wheat cultivars: Their relation to bread-making quality (1980)
    Springer
    Theoretical and applied genetics 58 (1980), S. 107-111 
    Details
    ISSN: 1432-2242
    Keywords: Glutenin ; Wheat ; Bread-making ; Chromosomal control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The subunit composition of glutenin from 47 European wheat cultivars was studied using SDS-polyacrylamide gel electrophoresis. These cultivars are genetically related since they originate from the same stock. Moreover, the diversity of sample, containing cultivars with very different French bread-making qualities, makes it possible to investigate the relationship between glutenin subunit composition and bread-making quality. 16 electrophoretic types of glutenin subunits could be distinguished: these were grouped into four classes. Depending on the cultivar, six to eight glutenin subunits with MW more than or equal to 62,000 were detected. Subunits 3 and 5, with an approximate MW of 122,000 and 108,000 respectively, seem to play a prominent role on bread-making quality; they were found in cultivars of good quality and were absent in those unsuitable for making French bread. Two other subunits (9 and 10; MW: 71,000 and 66,000, respectively) have a less defined influence but may be needed in some types of glutenin structure. Aneuploid analysis shows that in ‘Chinese Spring’, subunit 5 is coded by a gene on the long arm of chromosome 1B. The location of genes coding for subunits 3, 9 and 10 could not be determined.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00263099
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 31
    Electronic Resource
    Electronic Resource
    Rye chromosome translocations in hexaploid wheat: a re-evaluation of the loss of heterochromatin from rye chromosomes (1980)
    Springer
    Theoretical and applied genetics 56 (1980), S. 17-23 
    Details
    ISSN: 1432-2242
    Keywords: Wheat ; Rye ; Triticale ; Highly repeated ; DNA sequences ; Heterochromatin ; Translocations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Using in situ hybridization techniques, we have been able to identify the translocated chromosomes resulting from whole arm interchanges between homoeologous chromosomes of wheat and rye. This was possible because radioactive probes are available which recognize specific sites of highly repeated sequence DNA in either rye or wheat chromosomes. The translocated chromosomes analysed in detail were found in plants from a breeding programme designed to substitute chromosome 2R of rye into commercial wheat cultivars. The distribution of rye highly repeated DNA sequences showed modified chromosomes in which (a) most of the telomeric heterochromatin of the short arm and (b) all of the telomeric heterochromatin of the long arm, had disappeared. Subsequent analyses of these chromosomes assaying for wheat highly repeated DNA sequences showed that in type (a), the entire short arm of 2R had been replaced by the short arm of wheat chromosome 2B and in (b), the long arm of 2R had been replaced by the long arm of 2B. The use of these probes has also allowed us to show that rye heterochromatin has little effect on the pairing of the translocated wheat arm to its wheat homologue during meiosis. We have also characterized the chromosomes resulting from a 1B-1R translocation event. From these results, we suggest that the observed loss of telomeric heterochromatin from rye chromosomes in wheat is commonly due to wheat-rye chromosome translocations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00264422
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 32
    Electronic Resource
    Electronic Resource
    Aneuploid analysis of low molecular weight gliadins from wheat (1980)
    Springer
    Theoretical and applied genetics 56 (1980), S. 65-69 
    Details
    ISSN: 1432-2242
    Keywords: Wheat ; Gliadins ; Chromosomal control ; Aneuploid analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The chromosomal location of genes affecting five components from the low molecular weight gliadin (LMWG) fraction from wheat endosperm has been investigated by aneuploid analysis. Genes controlling these proteins were assigned to chromosomes 4B, 7A and 7D. Chromosomes from homoeologous groups 1, 2 and 6, where genes controlling classical gliadins are located, are not involved in the control of LMWG.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00264427
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 33
    Electronic Resource
    Electronic Resource
    Restoration of fertility in cytoplasmic male sterile (CMS) Nicotiana Sylvestris by fusion with X-irradiated N. tabacum protoplasts (1980)
    Springer
    Theoretical and applied genetics 58 (1980), S. 121-127 
    Details
    ISSN: 1432-2242
    Keywords: Nicotiana ; Cytoplasmic-male-sterility ; Somatic hybridization ; Plastid segregation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Restoration of male fertility was achieved by fusing protoplasts from male sterile (CMS) Nicotiana sylvestris plants with X-irradiated protoplasts derived from fertile N. tabacum plants. The CMS N. sylvestris plants were derived from a previous somatic hybridization experiment and contained alien (Line 92) cytoplasm. About one quarter of the regenerated plants were found to be cybrids. i.e. they consisted of N. sylvestris nuclei combined with all or some components of N. tabacum cytoplasm. In one half of these cybrids male fertility was restored to different levels. The chloroplasts of the two parental donors differ in respect to tentoxin sensitivity: chloroplasts of CMS N. sylvestris are sensitive while those of N. tabacum are insensitive. It could therefore be demonstrated that there was an independent segregation of chloroplast type and male fertility/sterility: several somatic cybrids were male fertile but tentoxin sensitive and others were tentoxin insensitive yet they were male sterile. Only in about one half of the somatic cybrids was male fertility restored together with restoration to tentoxin insensitivity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00263102
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 34
    Electronic Resource
    Electronic Resource
    Genotypic effects of the maternal tissues of wheat on its grain weight (1980)
    Springer
    Theoretical and applied genetics 58 (1980), S. 247-252 
    Details
    ISSN: 1432-2242
    Keywords: Maternal tissues ; Grain weight ; Wheat ; Triticum aestivum ; Caryopsis ; Pericarp ; Endosperm ; Embryo
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Reciprocal crosses were made between semi dwarf spring wheat cultivars (Triticum aestivum L.) differing in grain weight. The weights of the F1 grains (on maternal spikes), from intact as well as from defoliated plants, and those of the F2 grains (on f1 spikes), were examined. Grain weight was controlled primarily by the genotype of the maternal tissues (pericarp, testa or other floret or spikelet organs, including the vascular system), with complete dominance of heaviness. No indications suggesting maternal inheritance were obtained. The frequency distribution of the weights of the F2 grains indicated the presence of genotypic effects exerted by the endosperm or embryo. The embryo or endosperm factors for heaviness also seemed to be dominant.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00265174
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 35
    Electronic Resource
    Electronic Resource
    Vasopressin- and oxytocin-containing fibres in the pineal gland and subcommissural organ of the rat (1980)
    Springer
    Cell & tissue research 205 (1980), S. 11-17 
    Details
    ISSN: 1432-0878
    Keywords: Pineal organ ; Subcommissural organ ; Vasopressin ; Oxytocin ; Vasotocin ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Vasopressin and oxytocin were specifically demonstrated in the rat brain using the unlabelled antibody-enzyme method and purification of the first antiserum. Vasopressin and oxytocin fibres extend via the subcommissural organ or habenular commissure into the pineal stalk and terminate in the anterior part of the pineal organ. In addition, immediately adjacent to the subsommissural organ many vasopressin-containing fibres run caudally toward the central grey. These results are discussed in relation to the proposed presence of vasotocin in the pineal gland.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234438
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 36
    Electronic Resource
    Electronic Resource
    VIP (Vasoactive Intestinal Polypeptide)-immunoreactive neurons in the retina of the rat (1980)
    Springer
    Cell & tissue research 210 (1980), S. 167-170 
    Details
    ISSN: 1432-0878
    Keywords: Neuropeptides ; VIP-immunoreactive neurons ; Retina ; Amacrine cells ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Immunoreactive vasoactive intestinal polypeptide (VIP) was detected in a population of amacrine cells in the retina of the rat. Processes of these cells reach both the inner and outer half of the inner plexiform layer where they form sublayers. The VIP neurons are different from previously known amacrine cell types.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232152
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 37
    Electronic Resource
    Electronic Resource
    Simultaneous monoamine histofluorescence and neuropeptide immunocytochemistry (1980)
    Springer
    Cell & tissue research 210 (1980), S. 181-189 
    Details
    ISSN: 1432-0878
    Keywords: Catecholamines ; Neurophysin ; Simultaneous demonstration ; Functional interaction ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary A method was developed that allows the analysis of neuropeptides and monoamines in a single tissue section by the application of the unlabeled antibody method for peptide staining to tissue sections freeze-dried for formaldehyde-induced monoamine histofluorescence. The hypothalamic magnocellular system of male albino rats served as a model for this study; neurons were stained with anti-neurophysin sera, which mark the vasopressin- and oxytocin-associated proteins. Neurophysin-containing perikarya appeared to be surrounded by catecholamine-containing varicosities. This phenomenon was seen to varying degrees within the supraoptic and paraventricular nuclei. The juxtaposition of varicosities and peptidergic neurons suggests an afferent fiber-target neuron relationship that might favor a functional interaction between monoamines and neuropeptides.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00237608
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 38
    Electronic Resource
    Electronic Resource
    Ultrastructural alterations associated with the initiation of follicular atresia (1980)
    Springer
    Cell & tissue research 211 (1980), S. 105-115 
    Details
    ISSN: 1432-0878
    Keywords: Rat ; Preovulatory follicle ; Ultrastructure ; Degeneration ; Atresia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary To identify and describe ovarian follicles committed to undergo follicular degeneration (atresia), immature rats were primed with pregnant mare serum gonadotropin (PMSG). After PMSG treatment, preovulatory follicles develop but subsequently degenerate. Prior to the appearance of pyknotic nuclei (Stage I of atresia), degenerative changes were observed in focal areas of the granulosa cell layer. These changes include “blebbing” of the cytoplasm and alterations in the shape of the granulosa cells. The appearance of these degenerative changes coincides with a decrease in ovarian concentrations of estradiol and testosterone. Since estrogens and androgens maintain the follicle, the decline in estradiol and testosterone could be responsible for the further degenerative alterations that lead to complete deterioration of the preovulatory follicle. In Stage I atretic follicles, lysosome-derived autophagic vacuoles develop and macrophages invade both the thecal and granulosa cell layers. The combined actions of the autophagic vacuoles and macrophages could destroy both the granulosa-cell and thecal layers and thereby transform the preovulatory follicle into an ovarian cyst.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233727
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 39
    Electronic Resource
    Electronic Resource
    Depletion of macrophages and disappearance of postcapillary high endothelial venules in lymph nodes deprived of afferent lymphatic vessels (1980)
    Springer
    Cell & tissue research 211 (1980), S. 375-389 
    Details
    ISSN: 1432-0878
    Keywords: Lymph node ; Macrophages ; Postcapillary high endothelial venules ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The afferent lymphatic vessels of rat popliteal lymph nodes were interrupted, and the histological alterations in the lymph nodes occurring 1 to 14 weeks after operation were studied. One week after operation the number of macrophages was considerably reduced and continued to decrease during the subsequent time periods studied. A 6 weeks most macrophages had disappeared. Simultaneously the immunological activity diminished and had completely disappeared 8 weeks after operation. Three weeks after operation the endothelial cells of the postcapillary high endothelial venules had flattened, and the number of immigrating lymphocytes was greatly reduced. Subsequently the lymph nodes became depleted of both macrophages and lymphocytes, leaving only the reticuloendothelial framework.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234394
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 40
    Electronic Resource
    Electronic Resource
    A scanning electron microscope study of myoepithelial cells in exocrine glands (1980)
    Springer
    Cell & tissue research 209 (1980), S. 1-10 
    Details
    ISSN: 1432-0878
    Keywords: Myoepithelial cell ; Exocrine gland ; Scanning electron microscopy ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary By removing connective tissue components with enzymatic digestion followed by HCl-hydrolysis, myoepithelial cells (MECs) of the terminal portion in a variety of exocrine glands of the rat were examined with the scanning electron microscope. The profile of MECs varied considerably from gland to gland; MECs in the lactating mammary gland have a few long cytoplasmic processes in close contact with those of adjacent cells forming a continuous network around the terminal portion. Those of the exorbital lacrimal gland are stellate with many thin radiating processes with tapered ends that terminate freely. MECs in the sublingual gland are characterized by a number of broad and extensive cellular processes. MECs in the submandibular gland are similar in appearance to those of the exorbital lacrimal gland, but with more extensive cellular processes that form a more or less continuous network with those of the adjacent cells. No MECs were observed on the terminal portion of the parotid gland where the cells appear to be lodged on the intercalated duct. The relative surface area covered by MECs per terminal portion was also found to vary significantly, being 24% in the lactating mammary, 17% in the exorbital lacrimal, 48% in the sublingual, and 25% in the submandibular glands. The findings are discussed in relation to the physical properties of secretions in different glands.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00219918
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 41
    Electronic Resource
    Electronic Resource
    Nongranular vasopressin synthesis and transport in early stages of rehydration (1980)
    Springer
    Cell & tissue research 207 (1980), S. 89-107 
    Details
    ISSN: 1432-0878
    Keywords: Rehydration ; Nongranular vasopressin ; Intercellular clefts ; Axoplasmic reticulum ; Ultrastructural immunocytochemistry ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The vasopressin system of the rat was examined in the course of the first 12 h of rehydration after prolonged thirst at light and electron microscopic levels and by use of the peroxidase anti-peroxidase (PAP) method. Light microscopically, the median eminence was the only part of the system that not only displayed distinct differences between animals of different rehydration times but also showed a characteristic pattern of immunohistochemical reactivity in its rostro-caudal distribution. Ultrastructurally, in the perikarya a maximal labeling of the rough endoplasmic reticulum was observed after 2 h of rehydration, whereas an extensive labeling of the enlarged Golgi zones was attained after 4 h of resupplying water. A labeling of the intercellular clefts in the basal glial labyrinth of the supraoptic nucleus (and to a lesser degree in the subependymal neuropil adjacent to the paraventricular nucleus) was increased 30 min after the onset of drinking, as compared with water-deprived animals; it decreased slightly after 12 h of rehydration. The filling of the swollen fibers by increasing amounts of labeled axoplasmic reticulum, evident in the nuclear areas already after 30 min of water supply, begins in the median eminence after 2 h of rehydration and is fully developed after 4 and 8 h. Corresponding results hold true for the neural lobe but are somewhat delayed in comparison to the findings in the median eminence. The discussion considers (i) synthesis and transport of nongranular vasopressin within the axoplasmic reticulum, and (ii) release not only from the neural lobe but also from the nuclear areas and from the fibers of the median eminence.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00239332
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 42
    Electronic Resource
    Electronic Resource
    Functional reinnervation of the neostriatum in the adult rat by use of intraparenchymal grafting of dissociated cell suspensions from the substantia nigra (1980)
    Springer
    Cell & tissue research 212 (1980), S. 39-45 
    Details
    ISSN: 1432-0878
    Keywords: Neuronal grafting ; Dopamine ; Nigrostriatal system ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Dissociated cell suspensions were prepared from the substantia nigra of 15–17 day-old rat embryos and grafted via an intraparenchymal injection into the depth of the neostriatum of adult recipient rats. The survival and fibre outgrowth of the dopamine-containing neurones in the implants were studied by fluorescence histochemistry, and the functional capacity of the grafts was monitored by repeated testing of the amphetamine-induced turning behaviour of the implanted rats. Before transplantation the target neostriatum of the recipient rats was denervated of its normal dopaminergic innervation by an injection of 6-hydroxydopamine into the ipsilateral nigrostriatal dopamine pathway. The completeness of the denervation was ascertained by measurement of the intensity of the amphetamine-induced turning response. After injection of the dissociated cells large numbers of dopamine-containing neurones were found in clusters at the site of injection as well as scattered in the apparently intact neostriatal tissue up to a distance of about 0.5 mm from the site of injection. Extensive dopamine-containing fibre networks had developed around the implant. These newly formed fibres, which were most abundant around the cell clusters at the injection site, extended in a loose network into large areas of the initially denervated caudate-putamen. In all animals with surviving dopamine neurones the amphetamine-induced turning response was reduced, and in the most extensively reinnervated cases even reversed, within 3–5 weeks after transplantation. This strongly suggests that the implanted dopamine neurones are capable of restoring dopaminergic neurotransmission in the denervated neostriatum, probably via reinnervation of the denervated neostriatal tissue. The use of dissociated brain tissue preparations thus permits reliable intraparenchymal grafting of neurones to plausibly any desired site within the central nervous system, and should open entirely new possibilities for investigation of neuronal growth dynamics and functional reconstruction of damaged brain circuits, perhaps even in brains of larger mammals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234031
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 43
    Electronic Resource
    Electronic Resource
    A peculiar fibrillar pattern in the myocardial cells of trabeculae carneae in the right ventricle of the rat, mouse, and rabbit (1980)
    Springer
    Cell & tissue research 208 (1980), S. 165-168 
    Details
    ISSN: 1432-0878
    Keywords: Cardiac muscle cells ; Trabeculae carneae ; Fibrillar pattern ; Mouse ; Rat ; Rabbit
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The cardiac muscle fibers in the trabeculae carneae of mice, rats, and rabbits show a special arrangement of densely interwoven myofibrils. They cross at various angles; however, a preferred orientation of the fibrils cannot be discerned. It is suggested that due to this arrangement the myocytes of trabeculae are not able to contract to the same extent as ventricular myocytes, but thereby gain a high rigidity during contraction. Hence, they may play a principal role as “guiding ridges” for the flow of blood, thereby improving hemodynamics.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234181
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 44
    Electronic Resource
    Electronic Resource
    The influence of high pressure freezing on mammalian nerve tissue (1980)
    Springer
    Cell & tissue research 209 (1980), S. 201-216 
    Details
    ISSN: 1432-0878
    Keywords: High pressure (2,100 bars) freezing ; Freeze-etching ; Nerve tissue ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Vitrification of biological specimens in liquid nitrogen can be achieved under high pressure (2,100 bars). This procedure obviates the use of aldehyde fixation and cryoprotection (glycerol). The present work demonstrates its applicability to the freeze-etching of mammalian brain tissue. Freeze-fracture replicas from rat cerebellar cortex and subfornical organ prepared by this method are compared to conventionally processed material using aldehyde fixation, glycerination and freezing with Freon. The formation of large ice crystals is prevented in tissue blocks up to 0.5 mm thick; deep etching is markedly enhanced. Cytoplasmic microstructures such as mitochondrial cristae, microtubules and microfilaments, are readily observable against a finely granulated cytosol matrix. An additional advantage is the combined application with freeze-substitution.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00237626
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 45
    Electronic Resource
    Electronic Resource
    The structure of cleft material in spine synapses of rat cerebral and cerebellar cortices (1980)
    Springer
    Cell & tissue research 206 (1980), S. 477-486 
    Details
    ISSN: 1432-0878
    Keywords: Synapse ; Synaptic cleft ; Aldehyde ; PTA technique ; Densitometry ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Synaptic clefts of rat cerebral and cerebellar axodendritic spine synapses were studied after aldehyde-perfusion and subsequent immersion into osmic acid or after processing by the aldehyde-PTA technique. The threedimensional examination of aldehyde-perfused, osmic acid postfixed synapses revealed a double-layered intracleft lamina comparable in dimensions and position to the cleft density of non-osmicated, PTA-stained synapses. The relationship of this lamina to perisynaptic astroglial processes was pointed out. Densitometric analysis of the cleft area suggested the identity of the intracleft lamina of osmicated synapses with the cleft density of non-osmicated, PTA-stained synapses.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00237976
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 46
    Electronic Resource
    Electronic Resource
    Studies on the juxtaglomerular apparatus VI. Sympathetic innervation, catecholamines and the renin-angiotensin-system in rats and tree-shrews (Tupaia belangeri) (1980)
    Springer
    Cell & tissue research 212 (1980), S. 375-382 
    Details
    ISSN: 1432-0878
    Keywords: Juxtaglomerular apparatus ; Sympathetic innervation ; Renin-angiotensin system ; Electron microscopy ; Fluorescence microscopy ; Tupaia belangeri ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary It has previously been reported that the primitive primate Tupaia belangeri develops a renal failure when exposed to psychosocial stress. In order to learn if this high susceptibility to stress of the Tupaia kidney can be correlated with morphological and functional parameters of the Juxtaglomerular apparatus (JGA) and the renin-angiotensin system, comparative experiments were performed on Tupaia and rat. Our results reveal an outstandingly high potency of the JGA and the renin-angiotensin system in Tupaia as evident from the following findings: The Tupaia JGA contains a great number of epithelioid cells abounding in renin granules (electron microscopy). The renin content of the Tupaia kidney is considerably higher than in the rat (radio-immunoassay). The sympathetic innervation of the kidney and especially of the JGA is abundant in Tupaia (fluorescence and electron microscopy). Catecholamine contents of the kidney and other organs are significantly higher in Tupaia than in rats (spectrophotofluorometry). Our results support the previously developed concept of a potent intrarenal neuroendocrine interaction at the JGA level favouring, under certain conditions of social stress, the development of acute renal failure in Tupaia belangeri.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00236504
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 47
    Electronic Resource
    Electronic Resource
    Ultrastructural alterations in the initial segments and in the recurrent collateral terminals of Purkinje cells following axotomy (1980)
    Springer
    Cell & tissue research 212 (1980), S. 415-427 
    Details
    ISSN: 1432-0878
    Keywords: Cerebellum ; Purkinje axon ; Ultrastructure ; Axotomy ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Transection of Purkinje cell axons in adult male rats made 1.5 mm or further from the cell body does not lead to the death of the neuron and results in compensatory structural alterations of the surviving axonal portions of the nerve cell. Near to, and at the emergence of recurrent collaterals of Purkinje cell axons, huge varicosities filled with filaments, granular material, lysosomes and mitochondria develop. Terminals of recurrent axon collaterals also exhibit different degrees of structural changes. Most striking of the morphological alterations is the regular presence of nematosomes in the hypertrophic axonal branches, especially in synaptic terminals. Since nematosomes were shown to contain RNA in other types of neurons, their presence in recurrent collaterals may indicate an enhanced synthetic activity in Purkinje axonal processes and endings after axotomy.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00236507
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 48
    Electronic Resource
    Electronic Resource
    Interlamellar tight junctions of central myelin (1980)
    Springer
    Cell & tissue research 213 (1980), S. 81-94 
    Details
    ISSN: 1432-0878
    Keywords: Interlamellar tight junctions ; Central myelin ; Myelogenesis ; Optic nerve ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The process of myelination in the central nervous system (CNS) of the rat (optic nerve) was studied with the freeze-fracturing technique and ultrathin sectioning to obtain information on the developmental mechanisms of interlamellar tight junctions. Using a tilting cartridge for analysis of thin sections, it could be demonstrated that during the initial phase of wrapping a tight junction formation develops between the joining tips of the oligodendrocytic process. In tannic acid-stained samples these junctions appear as typical quintuple-layered membrane fusions, while in potassium permanganate-stained material membrane thickenings between the apposing glial tips are prevalent. The latter configuration represents the characteristic feature of the so-called radial component of central myelin. Using the freeze-fracturing technique, a biphasic mode of the myelinic tight junction assembly was detected. It is suggested that tight junctions represent a prerequisite of the myelination process.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00236922
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 49
    Electronic Resource
    Electronic Resource
    Dry matter content and mineral composition of potatoes grown in Cyprus (1980)
    Springer
    Potato research 23 (1980), S. 371-375 
    Details
    ISSN: 1871-4528
    Keywords: nitrogen ; phosphorus ; potassium ; calcium ; sodium ; nitrate ; sulphate ; micronutrients
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The specific gravity and dry matter content of 193 tuber samples (cultivars: Arran Banner, Spunta, Up-to-Date, Cara) from crops grown at Kokkinochoria, the major potato growing area of Cyprus, were related to each other as follows: Dry matter (%)=−269.59+268.24×specific gravity. This regression accounted for 82.4% of the variation; the fiducial limits (P〈0.05) for single determinations of specific gravity were±1.54 percentage units. The mineral contents of the tubers were similar to those reported in other countries.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02360677
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 50
    Electronic Resource
    Electronic Resource
    Purification of lumicolchicine for use in studies of plant development (1980)
    Springer
    Protoplasma 103 (1980), S. 105-114 
    Details
    ISSN: 1615-6102
    Keywords: Colchicine ; Lumicolchicine ; Microtubules ; Mitosis ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Lumicolchicine was purified by preparative thin-layer chromatography. Tests for purity were ultraviolet absorption spectrophotometry, analytical thin-layer chromatography, and a bioassay using wheat roots. Wheat roots treated for 3 days with 10−3 M lumicolchicine showed no c-mitosis, but had reduced growth compared with controls.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01276669
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 51
    Electronic Resource
    Electronic Resource
    Selection of embryogenic pollen from cold-treated buds ofNicotiana tabacum var. badischer burley and their development into embryos in cultures (1980)
    Springer
    Protoplasma 105 (1980), S. 161-167 
    Details
    ISSN: 1615-6102
    Keywords: Embryogenic pollen ; Nicotiana ; Pollen culture ; Selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary By using density gradient centrifugation, employing 55% percoll and 4% sucrose as suspension medium, it is possible to select embryogenic pollen from buds after cold treatment at 10 °C for 8 or more days. These buds at the uninucleate stage of pollen were collected from plants grown in 8 hours photocycles at 18 °C and supplied with mineral salts. The embryogenic pollen are small, starch-free with a clear cytoplasm whereas large starch-filled ones are nonembryogenic. The embryogenic pollen regularly form embryos at a frequency of 2% on a mineral medium supplemented with glutamine, asparagine and sucrose at pH 6.5. These results demonstrate, for the first time, that it is possible to have embryos in appreciable frequencies in “ab initio” pollen cultures raised from cold treated anthers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01279858
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 52
    Electronic Resource
    Electronic Resource
    Assimilation of uranium by wheat and tomato plants (1980)
    Springer
    Plant and soil 55 (1980), S. 55-59 
    Details
    ISSN: 1573-5036
    Keywords: Assimilation ; Deficiency ; Irrigation ; Soil ; Tomato ; Toxicity ; Uranium concentration ; Wheat ; Yield
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Greenhouse conditions have been used for the study of uptake of uranium by wheat and tomato plants as affected by its concentration in soil and irrigation applied. The highest yield of wheat was obtained at 3.0 ppm of uranium whereas the tomato yield decreased with the increase of uranium in the soil. The analysis shows that Uranium uptake by wheat and tomato not only depends upon the uranium concentration in the soil but also on the amount of irrigation applied.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02149708
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 53
    Electronic Resource
    Electronic Resource
    The development of waterlogging damage in wheat seedlings (Triticum aestivum L.) (1980)
    Springer
    Plant and soil 54 (1980), S. 77-94 
    Details
    ISSN: 1573-5036
    Keywords: Nutrients ; Oxygen ; Roots ; Soil ; Toxins ; Waterlogging ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effects of waterlogging on concentrations of gases and various solutes dissolved in the soil water were investigated in the laboratory, to determine whether the early disruption to the growth of wheat was most closely associated with depletion of dissolved oxygen, accumulation of toxins, or changes in concentrations of nutrient ions in the soil water. Waterlogging slowed shoot fresh weight accumulation, leaf extension and nodal root growth; it also caused death of the seminal root system and early senescence of the lower leaves. However, the shoot dry weight initially increased above that of the non-waterlogged controls, and thus was not a reliable indicator of the early restriction to plant growth and development. The symptoms of damage to shoots and roots were attributed to the fall in soil oxygen concentrations, rather than to any decrease in concentration of inorganic nutrients in the soil water, or to the accumulation of any other measured solutes to toxic concentrations.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02182001
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 54
    Electronic Resource
    Electronic Resource
    Root exudates of plants (1980)
    Springer
    Plant and soil 57 (1980), S. 69-83 
    Details
    ISSN: 1573-5036
    Keywords: Bacteria ; Concentration gradient ; Exudates ; Pseudomonas putida ; Root exudation ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The release of substances from wheat roots was found to be directly related to the growth of the root system. Plants whose root system did not grow released almost no exudates. When exudate concentration in the vicinity of the roots was lowered by frequent replacements of the nutrient solution or by a simultaneous cultivation of exudate-utilizing bacteria, the release of exudates was enhanced. In axenic wheat cultures, the amount of exudates during a 12-day cultivation with 2- or 4-day intervals between medium replacements represented 50% of root dry weight and 12% of whole plant dry weight. Wheat plants cultivated in the presence of the bacteriumPseudomonas putida released up to double the amount of exudates compared with axenic variants.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02139643
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 55
    Electronic Resource
    Electronic Resource
    Effect of soil temperature alteration by soil covers on seedling emergence of wheat (Triticum aestivum L.) sown on two dates (1980)
    Springer
    Plant and soil 57 (1980), S. 125-129 
    Details
    ISSN: 1573-5036
    Keywords: Mulch ; Polyethylene sheet ; Seedling emergence ; Soil temperature ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A field study was conducted to evaluate the effect of soil temperature altered by various ways of soil coveringviz control (T1), transparent polyethylene (T2), black polyethylene (T3), rice straw (T4), rice straw applied only during night (T5) and farm yard manure (T6) on seedling emergence of wheat sown on November 30, 1978 and January 6, 1979. Maximum increase in soil temperature occurred under transparent polyethylene cover. Temperature alterations were relatively smaller under other treatments and was minimum under farm yard manure cover. With both sowings, the effect of various ways of soil covering on seedling emergence depended on the magnitude of soil temperature alteration caused by them. It was hastened markedly under transparent polyethylene cover and only slightly under black polyethylene cover. Continuous soil covering with rice straw slowed down seedling emergence while covering only during night hastened it slightly. Farm yard manure cover showed negligible effect on seedling emergence. Seedling emergence response to an increase in soil temperature was higher with second sowing when temperature was relatively lower than with first sowing. The result suggests the possibility of expediting seedling emergence of wheat sown under low temperature condition by transparent polyethylene cover. Seedling emergence was only slightly influenced by other soil covers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02139648
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 56
    Electronic Resource
    Electronic Resource
    Assessment of a rapid method, using soil cores, for estimating the amount and distribution of crop roots in the field (1980)
    Springer
    Plant and soil 55 (1980), S. 297-305 
    Details
    ISSN: 1573-5036
    Keywords: Barley ; Cereals ; Root distribution ; Root growth ; Soil ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A study was made of the relationship between the number of roots (Nr) observed on unit area of the freshly exposed, horizontal faces of soil cores, and the amounts of roots (per unit volume) present in the same cores. Soil cores, 7 cm diameter, were extracted to depths of 1 m from cereal crops in 1976 at three field sites located on clay soils. Sampling was either at the start of stem elongation, or at anthesis. Estimates of root length per unit soil volume (L) were derived from Nr by assuming random orientation of roots in the soil. Values of L were found to be highly correlated with the measured lengths of both the main roots (root axes) and the total roots (axes and laterals) washed from the soil at a given growth stage, for each of the soils. On average, L was 3.3 times the length of root axes washed from the soil, and was 0.42 times the length of total roots, but there was appreciable variation between different growth stages and field sites. Possible factors giving rise to differences between L and the measured lengths of roots are discussed. Estimates of root length from observation of soil cores may nonetheless provide a suitable basis for rapidly comparing therelative distribution of roots down the soil profile under field conditions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02181809
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 57
    Electronic Resource
    Electronic Resource
    Thermal conductivity of nitrogen at high pressures (1980)
    Springer
    International journal of thermophysics 1 (1980), S. 375-381 
    Details
    ISSN: 1572-9567
    Keywords: thermal conductivity ; high pressure ; nitrogen ; computer simulation ; modified Enskog theory
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract The results of the measurements of the thermal conductivity coefficients of nitrogen at 298.15 K from atmospheric pressure up to 1 GPa are reported. The experimental values are used to test the Modified Enskog Theory and the corresponding state principle. The experimental values are also compared with the results of computer simulation of the thermal conductivity of a Lennard Jones fluid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00516564
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 58
    Electronic Resource
    Electronic Resource
    An evaluation of seed and seedling drought tolerance screening tests in wheat (1980)
    Springer
    Euphytica 29 (1980), S. 727-736 
    Details
    ISSN: 1573-5060
    Keywords: Wheat ; Triticum aestivum ; bread wheat ; Triticum durum ; durum wheat ; drought tolerance ; germination ; seedling growth ; water stress selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A series of experiments was performed in order to evaluate the significance of seed germination and seedling growth in osmotic media as screening methods for drought tolerance. Ten spring wheat (Triticum aestivum L. em Thell.) and one durum wheat (Triticum durum Desf.) were tested under controlled environments, using polyethylene glycol-6000 (PEG) solutions as the moisture stress inducing media. Tolerance in the rate of endosperm utilization, under stress, prior to the onset of germination varied among cultivars. Germination rate or injury to germination at various concentrations of PEG differed significantly among cultivars. Cultivar rating with respect to injury to germination changed with stress levels. Injury to germination did not correlate with endosperm utilization rate in PEG or in water. Germinating seedlings were tolerant to extreme desiccation up to the stage of emergence of the first leaf from the coleoptile. Growth of photosynthesizing seedlings was monitored as they were carried through an increasing concentration gradient of PEG solutions, ranging from −5.9 to −11.3 bars of water potential. Cultivars significantly differed in seedling growth tolerance to increasing levels of water stress. Seedling growth tolerance across cultivars was not correlated with their germination responses under srress. It is concluded that tolerance to water stress in growing seedlings can be screened for by using PEG-containing nutrient solutions. It can not be predicted from germination tests in osmotica. Work was done under a US-Israel Binational Science Foundation (BSF) Grant no. 1654/78.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023219
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 59
    Electronic Resource
    Electronic Resource
    Studies on biological decomposition of wheat straw (1980)
    Springer
    Plant and soil 55 (1980), S. 235-242 
    Details
    ISSN: 1573-5036
    Keywords: Decomposition ; Groundnut ; Soil properties ; Wheat ; Wheat straw ; Yield
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The incorporation of undecomposed wheat straw in the soil along-with the micro-organisms favourably increased the yield of groundnut crop. An increase of 37 per cent in yield was recorded when wheat straw was inoculated withPenicillium digitatum and the C:P ratio was adjusted to 65. Inoculated treatments of narrower C:P ratio gave a higher yield than wider C:P ratio treatments inoculated with the same cultures. An increase in nitrogen uptake by groundnut plants was recorded due to incorporation of straw alongwith the micro-organisms in soil. The organic carbon and nitrogen content of the soil increased with all the treatments except control. The highest increase in organic carbon and nitrogen of the soil was observed with a treatment of wheat straw of 65 C:P ratio inoculated withS. coccosporum. The yield of wheat crop after groundnut was significantly more with several treatments than control plots. The highest increase of 79 per cent in grain yield of wheat was observed in the plots previouslq received with wheat straw of 200 C:P ratio.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02181803
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 60
    Electronic Resource
    Electronic Resource
    Growth and development of root systems of winter cereals grown after different tillage methods including direct drilling (1980)
    Springer
    Plant and soil 55 (1980), S. 283-295 
    Details
    ISSN: 1573-5036
    Keywords: Clay soils ; Cultivations ; Direct drilling ; Earthworms ; Root growth ; Root sampling ; Soil water content ; Soil structure ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A method is described for rapidly estimating the depth of penetration and density of roots of cereal crops under field conditions. Counts of living roots, traversing horizontal faces of soil cores, were made for winter wheat growing on direct-drilled and ploughed land. The rate of penetration of roots of winter wheat in a clay and a sandy loam soil averaged 5 mm per day throughout winters without extremes of cold or wet. Death of roots near the soil surface occurred wilst others continued downward penetration. The rate of root elongation was slower during prolonged periods when the soil was wet and faster,i.e. to greater depths, during dry conditions. Damage sustained to roots during adverse winter conditions ofter varied between direct drilling and ploughing. More roots at depth were consistently recorded on direct-drilled than on ploughed land when measured in spring after a soil water deficit had developed during the preceding month. After prolonged wet soil conditions during the winter on a soil with a large clay fraction and low hydraulic conductivity, root growth and penetration in spring, before the development of a soil water deficit, was more restricted on direct-drilled than on ploughed land.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02181808
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 61
    Electronic Resource
    Electronic Resource
    The occrrence of vesicular-arbuscular mycorrhiza in barley and wheat grown in some Danish soils with different fertilizer treatments (1980)
    Springer
    Plant and soil 55 (1980), S. 403-414 
    Details
    ISSN: 1573-5036
    Keywords: Infection intensity ; Loamy soil ; N fertilizer ; P fertilizer ; Sandy soil ; VA-mycorrhiza ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Soil samples, roots and shoots were collected from barley crops at three locations which had received different combinations of N and P for 10 years, and from long-term fertilizer experiments on barley in a sandy soil and on barley and wheat in a loamy soil. Soils were analysed for available P by an anion exchange resin procedure, roots were examined for intensity of VAM infection, and shoots wee analysed for N and P. Vesicualr-arbuscular mycorrhizal infection was found at all locations. It was most abundant at the three locations with least soil-P and lightest at the two locations high in soil-P. Within loocations an inverse relation was found between soil-P level and intensity of infection. Infection was also intensity. by increasing N-fertilizer. Spore counts from selected samples correlated well with infection intensity. Shoot-P did not differ significantly between treatments in spite of significant differences in soil-P. This points to the significance of vesicular-arbuscular mycorrhiza: a lower soil-P level accompanied by a higher infection intensity seem to counterbalance each other to a certain extent.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02182701
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 62
    Electronic Resource
    Electronic Resource
    pH optima for crop growth (1980)
    Springer
    Plant and soil 54 (1980), S. 339-357 
    Details
    ISSN: 1573-5036
    Keywords: Calcium ; Cassava ; Chemical composition ; Control of solution pH ; Copper ; Flowing solution culture ; French bean ; Ginger ; Hydrogen ion injury ; Magnesium ; Maize ; Manganese ; Nitrogen ; Optimum pH range ; pH ; Plant growth ; Root weight ratio ; Tomato ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Ginger, cassava, maize, wheat, french bean and tomato were grown for periods up to six weeks in continuously flowing nutrient solutions at seven constant pH values ranging from 3.3 to 8.5. All species achieved maximum or near-maximum growth in the pH range 5.5 to 6.5. However, there were substantial differences in the ability of species to grow outside this range. Ginger and cassava were the most tolerant species to low solution pH, while ginger and tomato were the only species to show no yield depression at the highest solution pH. Roots of all species at pH 3.3 and some species at pH 4.0 exhibited symptoms of hydrogen ion injury. In addition, the concentrations of magnesium in the tops of all six species, of nitrogen in the tops of tomato and cassava, and of manganese in the tops of maize at these pH values were inadequate for optimal growth. Growth depression at high solution pH was associated with iron deficiency in maize and wheat and with nitrogen and/or copper deficiency in cassava. The relevance of the present results to crop growth under field conditions is discussed. The complex interplay of plant and soil characteristics militates against precise definition of an optimum pH range for the growth of a particular crop unless the soil is also specified.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02181830
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 63
    Electronic Resource
    Electronic Resource
    Effect of concentration of boron on the uptake and yield of tomato and wheat at different levels of irrigation (1980)
    Springer
    Plant and soil 54 (1980), S. 479-484 
    Details
    ISSN: 1573-5036
    Keywords: Boron concentration ; Boron deficiency ; Greenhouse ; Irrigation ; Tomato ; Uptake ; Wheat ; Yield
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A study was conducted under greenhouse conditions to know the effects of irrigation levels on the uptake of B by wheat and tomato at different levels of B concentrations. The uptake was found to increase with increase in concentrations as well as with increase in amount of water applied. The highest yield of tomato was obtained at 1.5 ppm of B, whereas that of wheat was obtained at 3 ppm of B at the highest level of irrigation. The boron uptake by tomato and wheat is a function of both boron concentration in the soil and the amount of irrigation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02181840
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 64
    Electronic Resource
    Electronic Resource
    On the concentration of acetic acid in straw and soil (1980)
    Springer
    Plant and soil 56 (1980), S. 93-98 
    Details
    ISSN: 1573-5036
    Keywords: Acetic acid ; Barley ; Diffusion ; Silt loam ; Straw ; Water absorption ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Freshly harvested wheat straw contained 0.096 g water g−1 dry straw and 180 mM acetic acid. The straw absorbed water more rapidly from wet soil. The concentration of acetic acid fell to about 10 mM within 6 h of incorporation of straw in the soil and then remained relatively constant for a period of 12 days, irrespective of soil moisture content. In soil at its maximum water holding capacity after gravitational drainage, the decline in acetic acid concentration (c) with distance (d) from wheat or barley straw was exponential, with c=co e−nd where co is the concentration of acetic acid at the straw surface and n is a constant (0.46 for barley and 0.42 for wheat straw). The presence of acetic acid seems to be a major cause of poor establishment and growth when seeds and seedling roots come into contact with straw.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02197956
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 65
    Electronic Resource
    Electronic Resource
    Free proline accumulation in cereals in relation to salt tolerance (1980)
    Springer
    Plant and soil 57 (1980), S. 167-175 
    Details
    ISSN: 1573-5036
    Keywords: Accumulation ; Barley ; Cations ; Electrical conductivity ; K/Na ratio ; Proline ; Saline water ; Stress ; Tolerance ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In a micro-plot experiment it was observed that free proline accumulation in barley and wheat crops increased with the salt stress. But in sensitive strains of both the crops it did not accumulate in accordance with the preceding values after their tolerance limit and at this point yield of barley was reduced by 25.8% and wheat by 23.2% over control while significant yield reductions were not observed with tolerant strains. Proline accumulation was also found to be related to tissue K/Na ratio and the critical limits of this ratio were observed between 0.60 and 0.34 for barley and 4.2 and 1.4 for wheat.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02211676
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 66
    Electronic Resource
    Electronic Resource
    Aluminium effects on growth and Al, Ca, Mg, K and P levels in triticale, wheat and rye (1980)
    Springer
    Plant and soil 57 (1980), S. 467-470 
    Details
    ISSN: 1573-5036
    Keywords: Aluminum concentration ; Aluminum toxicity ; Calcium ; Magnesium ; Nutrient solution ; Phosphorus ; Potassium ; Triticale ; Rye ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effects of A1 on the growth and mineral composition of different cultivars of triticale (X Triticosecale, Wittmack), wheat (Triticum aestivum L.) and rye (Secale cereale L.) growing in 1/5 strength Steinberg solutions containing 0 or 6 ppm A1 were evaluated after 32 days. Aluminum increased the concentrations of P and K in the roots and K in the tops of most of the cultivars tested. A1 tolerant triticale retained a lower concentration of Mg in the roots and tops than the A1 sensitive triticale, when subjected to A1 stress. In addition, A1 treatments resulted in smaller increases in root P for the A1 tolerant triticale than for the A1 sensitive cultivars. The concentration of root Ca and P of the A1 tolerant wheat cultivars were significantly below that of the more sensitive plants. Aluminum tolerance in rye appeared to be associated with lower Ca and higher Mg concentrations in the tops. The accumulation of P and A1 in the roots was characteristic of sensitivity in triticale, wheat and rye.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02211704
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 67
    Electronic Resource
    Electronic Resource
    Wheat root distribution in sodic soils (1980)
    Springer
    Plant and soil 57 (1980), S. 487-490 
    Details
    ISSN: 1573-5036
    Keywords: Exchangeable sodium percentage ; Restricted root growth ; Sodic ; Soil ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Greenhouse experiment conducted on Ghabdan loam (a Salic Natraqualf), varying in ESP distribution of soil profile showed that wheat roots could develop having an ESP of 45. Above an ESP of 51 absolutely no root growth was observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02211708
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 68
    Electronic Resource
    Electronic Resource
    Annual changes of nitrogen and phosphorus in two aquatic macrophytes (Nymphaea Tuberosa and Ceratophyllum Demersum) (1980)
    Springer
    Hydrobiologia 70 (1980), S. 31-35 
    Details
    ISSN: 1573-5117
    Keywords: macrophyte ; nitrogen ; phosphorus ; nutrients
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The nitrogen and phosphorus content of Nymphaea tuberosa and Ceratophyllum demersum, in Lake Onalaska, Wisconsin, was studied for a year. On a yearly basis, N. tuberosa exhibited nitrogen and phosphorus differences among seasons and among plant parts. Variation among plant parts was also evident in C. demersum. However, within individual plant structures, no seasonal differences were observed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00015487
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 69
    Electronic Resource
    Electronic Resource
    An intermittent somatostatin-immunoreactivity in the cortex and basal ganglia of the rat (1980)
    Springer
    Cell & tissue research 205 (1980), S. 327-331 
    Details
    ISSN: 1432-0878
    Keywords: Somatostatin ; Cortical cells and fibers ; Immunohistochemistry ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Using light microscopic immunohistochemistry, somatostatinpositive structures were observed in the cortex of the rat. These structures, including cells and fibers, are widely distributed in all cortical laminae and are also found in the basal ganglia. The positive results were obtained exclusively in two groups of animals sacrificed during two different months of two subsequent years. The reason for this variability in the immunocytochemical stainability of cortical structures remains enigmatic.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234691
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 70
    Electronic Resource
    Electronic Resource
    Architecture of normal pancreas as revealed by retrograde injection (1980)
    Springer
    Cell & tissue research 205 (1980), S. 445-451 
    Details
    ISSN: 1432-0878
    Keywords: Exocrine pancreas ; Rat ; Architecture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The architecture of the pancreas was revealed by retrograde injection of the pancreatic ductal system of normal rats with a silicone rubber compound, and subsequent study of the preparation by light microscopy and scanning electron microscopy. The injected material became associated with both ducts and “acinar” areas. Examination of these specimens suggests that the arrangement of the exocrine pancreas is that of a complexly curving and branching system of tubules which anastomose and end blindly. This architecture, which is not that of a true acinar gland, provides a rational basis for the understanding of the simple dedifferentiative changes that accompany pancreatic carcinogenesis, and which have been generally interpreted as representing ductular proliferation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232285
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 71
    Electronic Resource
    Electronic Resource
    Electron microscopic immunocytochemical investigation on the postnatal development of the vasopressin system in the rat (1980)
    Springer
    Cell & tissue research 205 (1980), S. 453-471 
    Details
    ISSN: 1432-0878
    Keywords: Vasopressin system ; Postnatal development ; Ultrastructural immunocytochemistry ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The present ultrastructural results indicate that, in the rat, the vasopressin-synthesizing perikarya of the supraoptic nucleus (NSO) attain a certain degree of maturity earlier than those of the paraventricular nucleus (NPV). In the neonate rat, the stainability of the nuclear areas is very weak; in the perikarya of the NSO a few labeled granules can be found, whereas the perikarya of the NPV often display only a labeled Golgi area, the cytoplasm being devoid of granules. At the end of the first (NSO) and the second (NPV) postnatal weeks, the filling of the neurosecretory granules with vasopressin is inhomogeneous with irregular spots of reaction product distributed on the granules. This feature is less obvious during the following week and has nearly disappeared after the third and fourth postnatal weeks. Already in the neonate two types of vasopressin-positive fibers are observed in the median eminence, characterized by the different diameters of their granules and by their typical location in the internal and the external pericapillary contact zone. Especially in one and two week-old animals, in the internal zone of the median eminence and, to a lesser degree in the neural lobe, the immunocytochemical reaction product is deposited on an axonal tubular network. Judging from the presence of very few vasopressin-negative fibers in the neural lobe of the neonate, the development of the oxytocin system appears to be delayed. A characteristic relationship between pituicytes and the neurosecretory fibers can be observed during the first two postnatal weeks. After the third postnatal week the immunocytochemical features of the vasopressin system correspond approximately to that in adult rats.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232286
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 72
    Electronic Resource
    Electronic Resource
    Scanning electron microscopy of the cleft of the rat pituitary (1980)
    Springer
    Cell & tissue research 206 (1980), S. 139-143 
    Details
    ISSN: 1432-0878
    Keywords: Pituitary cleft ; Scanning electron microscopy ; Cell surface changes ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Scanning electron microscopy of the lining of the pituitary cleft was carried out in normal, lactating, castrated, adrenalectomized, and cyproterone-treated adult rats. Four cell types could be differentiated in the posterior wall in control and experimental animals: (1) cells with a smooth surface, (2) cells with microvilli located at the cellular borders, (3) ciliated cells, and (4) cells with evenly distributed microvilli. The anterior wall showed mainly cells with few microvilli located at their margins, and clusters of ciliated cells. In normal, and more frequently in experimental animals, the anterior wall showed shriveled cells, and variously sized cavities. Colloid appeared either as a network of finely granular material or as compact bodies adhering to the epithelial surface. These observations suggest that a compact component of the colloid is derived at least in part from degraded cells.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233614
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 73
    Electronic Resource
    Electronic Resource
    Presence of AVT-, α-MSH-, LHRH- and somatostatin-like compounds in the rat pineal gland and their relationship with the UMO5R pineal fraction (1980)
    Springer
    Cell & tissue research 206 (1980), S. 341-353 
    Details
    ISSN: 1432-0878
    Keywords: AVT ; LHRH ; α-MSH ; Somatostatin ; Pineal Gland ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Description / Table of Contents: Resumé En utilisant des anticorps contre l'AVT, l'α-MSH, le LHRH et la somatostatine, des cellules épiphysaires du Rat ont été immunocytochimiquement colorées. Tous ces anticorps colorent les mêmes cellules. Ces cellules réagissent également quand un anticorps est utilisé contre la fraction épiphysaire UMO5R, fraction qui est douée de propriétés antigonadotropiques in vivo. Il a également été montré que le nombre des cellules immunoréactives était plus important dans la pinéale du jeune rat que dans celle de l'adulte. La comparaison des résultats obtenus avec différents anticorps et l'étude des propriétés de ces anticorps aprés absorption sur différents peptides ou sur différentes fractions épiphysaires, a permis de conclure que les réactions obtenues dans la pinéale du rat n'étaient que la conséquence d'une réaction croisée de ces anticorps avec une/des substance(s) inconnue(s) synthétisée(s) par la pinéale elle-même. La nature endocrine possible de cette substance qui serait chimiquement apparenté aux fractions épiphysaires Mouton UMO5R et Prot. 4, est discutée. Drs. B.L. Baker (Ann Arbor, Mich., USA), M.P. Dubois (Nouzilly, France), J. De Mey (Beerse, Belgium), J.D. Fernstrom (Cambridge, Mass., USA.), H. Goos (Utrecht, The Netherlands), B. Kerdelhué (Gif-sur-Yvette, France) and A.G.E. Pearse (London, U.K.) are also acknowledged for their gifts of various antibodies
    Notes: Summary Using antibodies against AVT, α-MSH, LHRH and somatostatin, immunoreactive cells were detected in the rat pineal gland. All of these antibodies stain the same cells, which also react immunocytochemically when an antibody against the UMO5R sheep pineal fraction, a fraction that presents antigonadotropic properties in vivo, is used. Relatively more immunoreactive cells are present in the pineals of young rats than in the pineals of adult animals. Comparison of the results obtained with different potent antibodies against each of the peptides, and a study of the staining properties of the antibodies in the pineal after solid phase adsorption to different peptides or to different sheep pineal fractions, led to the proposal that the immunoreactivity found in the rat pineal is not due to the presence of AVT, α-MSH, LHRH or somatostatin, but to a cross-reaction of each of these antibodies with (an) unidentified compound(s). This compound is synthetized in the pineal gland, as was demonstrated using cultured pineals. The UMO5R and the Prot. 4 fractions of the sheep pineal seem to be chemically related to this unknown compound, the possible endocrine nature of which is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00237964
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 74
    Electronic Resource
    Electronic Resource
    Stimulation-depletion of serotonin and noradrenaline from vesicles of sympathetic nerves in the pineal gland of the rat (1980)
    Springer
    Cell & tissue research 207 (1980), S. 13-20 
    Details
    ISSN: 1432-0878
    Keywords: Sympathetic nerves ; Synaptic vesicles ; Noradrenaline ; Serotonin ; Pineal gland ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The pineal gland of the rat receives a rich nervous supply originating from the superior cervical ganglia. These fibers contain serotonin in addition to their neurotransmitter, noradrenaline. Cytochemical studies at the ultrastructural level have shown that both amines are present in the cores of the granular vesicles that are characteristic of these nerves. It is presently shown that the bilateral electrical stimulation of the preganglionic fibers innervating the ganglia markedly reduces the number of small sites reacting cytochemically for both noradrenaline and serotonin, these sites corresponding to the cores of small granular vesicles, while the larger reactive sites (cores of large vesicles) remain unaltered. The vesicles are retained in nerve terminals after stimulation, as observed in conventionally processed tissues, although with altered sizes and shapes. Apart from these cytochemical and structural changes, nerve stimulation also reduces the endogenous noradrenaline content of the pineal gland. Thus, both noradrenaline and serotonin are released from their storage sites in pineal sympathetic nerves after electrical stimulation in vivo. This suggests the possibility that several substances with presumed transmitter or modulatory functions might be simultaneously released by nerve impulses from a given nerve terminal.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00239325
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 75
    Electronic Resource
    Electronic Resource
    Organ culture of the islets of Langerhans from young and senescent rats (1980)
    Springer
    Cell & tissue research 207 (1980), S. 429-448 
    Details
    ISSN: 1432-0878
    Keywords: Islet of Langerhans ; Aging ; Insulin-secreting cells ; Organ culture ; Electron microscopy ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The B-cells of the endocrine pancreas constitute an adequate model for in vitro study of the aging process in highly differentiated cells. In the present study, collagenase-isolated islets of Langerhans from young and senescent rats were cultured up to 28 days. The response of the B-cells to the stimulatory conditions of the culture medium involved the nucleus, ribosomes, endoplasmic reticulum, Golgi apparatus, and secretory granules. Correlated data from light microscopy, electron microscopy, and insulin radioimmunoassay show that the differentiation and function of senescent B-cells are maintained in culture, as it has been proven for the B-cells of younger animals. On the other hand, signs of cytological deficiency not directly concerned with the specific function of B-cells were observed: abnormal mitochondria and lysosomes are more numerous in the senescent B-cells. The proliferative capacity of the B-cells of aged rats is reduced.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224618
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 76
    Electronic Resource
    Electronic Resource
    Dynamics of the transganglionic movement of horseradish peroxidase in primary sensory neurons (1980)
    Springer
    Cell & tissue research 207 (1980), S. 479-489 
    Details
    ISSN: 1432-0878
    Keywords: Horseradish peroxidase ; Primary afferent neurons ; Axonal transport ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The dynamics of horseradish peroxidase (HRP) transport in primary sensory neurons were studied in rats by demonstration of the reaction product in spinal nerves, spinal ganglia, dorsal roots and in the spinal cord at different survival times after application of the enzyme to the transected sciatic nerve and to the spinal cord. Using tetramethylbenzidine as the chromogen according to Mesulam (1978), transganglionic transport of HRP was shown in both the disto-proximal direction after peripheral application, and proximo-distal direction after central application. Significant differences in staining intensity between the central and peripheral processes of primary sensory neurons were found after all survival times used in this study. After peripheral application the number of labeled axons and the staining intensity were higher in spinal nerves than in dorsal roots; an inverse situation occurred after central application. These differences as well as the time sequences in staining of different parts of primary sensory neurons suggest that HRP applied to a peripheral nerve and to the spinal cord, respectively, enters the perikarya of spinal ganglion cells in any case before continuing its movement in a cellulifugal direction. Lysosomal degradation of the major portion of the applied HRP is supposed. However, in the post-perikaryal portion of a considerable number of neurons HRP-transport still occurs to a varying extent, thus resulting in labeling of nerve endings. In some neurons a post-perikaryal transport could not be detected light microscopically. The transport rates differ: the calculated transport rate of disto-proximal, cellulipetal movement in the fastest transporting neurons was 7.5 mm/h, that of the disto-proximal cellulifugal movement 2.5 to 3 mm/h.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224621
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 77
    Electronic Resource
    Electronic Resource
    Changes of the caecal villi during postnatal development in rats (1980)
    Springer
    Cell & tissue research 208 (1980), S. 253-259 
    Details
    ISSN: 1432-0878
    Keywords: Villus ; Caecum ; Postnatal development ; Rat ; Scanning electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The villi of the caecal mucosa in postnatal rats were studied using both scanning electron and light microscopy. On the day of birth, numerous villi of various sizes and shapes were present on the caecal mucosa. After the 5th day, the villi decreased very rapidly in length and in number. A strong constriction was observed at the basal region of the caecal villi. During postnatal days 5 ∼ 9 the villi probably separated and disappeared from the caecal mucosa. No villi were observed in rats that were over 10 days of age.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234875
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 78
    Electronic Resource
    Electronic Resource
    Hypothalamo-hypophysial function following the lesion of tanycytes in the median eminence of the rat (1980)
    Springer
    Cell & tissue research 209 (1980), S. 225-238 
    Details
    ISSN: 1432-0878
    Keywords: Tanycytes ; Median eminence ; Electron microscopy ; Rat ; Serum LH
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The transport of hormones from the cerebrospinal fluid to the adenohypophysis by the tanycytes of the median eminence was examined in male rats. Electron microscopy revealed that all ependymal cells including the tanycytes disappear or degenerate in rats subjected to electric cauterization of the ependymal layer lining the third ventricle. However, the granular axons in the palisade layer of the median eminence remain intact. In rats subjected to electric lesion, no significant change was found in either the serum-LH level or in the weight of the adenohypophysis, testes, adrenal and thyroid glands. It is concluded that the tanycytes do not participate in the hypothalamic regulation of hypophysial function.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00237628
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 79
    Electronic Resource
    Electronic Resource
    Immunohistochemical study of the LHRH-synthesizing neuron system of aged female rats (1980)
    Springer
    Cell & tissue research 209 (1980), S. 499-503 
    Details
    ISSN: 1432-0878
    Keywords: LHRH perikarya ; Aging ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The LHRH-synthesizing neuron system was studied in young proestrous and old female rats, and in aged ovariectomized or reserpine-treated females. The medial preoptic area and septal region of old animals contains more LHRH positive perikarya compared to that of young proestrous rats. Reserpine treatment moderately increases the number of immunostainable LHRH cells, while ovariectomy is ineffective in this respect.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234761
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 80
    Electronic Resource
    Electronic Resource
    Luliberin and somatostatin fiber-terminals in the subfornical organ of the rat (1980)
    Springer
    Cell & tissue research 210 (1980), S. 33-45 
    Details
    ISSN: 1432-0878
    Keywords: Luliberin (LRF)-terminals ; Somatostatin-terminals ; Subfornical organ ; Neurohemal regions ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary With the aid of light- and electron- microscopic immunocytochemistry, somatostatin- and luliberin (LRF)-positive fibers can be demonstrated in the rat subfornical organ (SFO). Each of the neurohormones has a specific location: LRF in the lateral parts of the organ, and somatostatin in the center of the posterior zone. Common to both neurohormone-containing fibers is the pattern in which they reach the organ as well as the fact that their terminals are located in the perivascular spaces of fenestrated vessels, i.e., within the limited neurohemal regions of the organ. Since injection of India ink of different colors demonstrates that the capillary bed of the SFO is connected with the central capillaries of the choroid plexus, the question arises as to whether the neurohormones released in the area of the SFO influence the choroid plexus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00232139
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 81
    Electronic Resource
    Electronic Resource
    Morphological investigation of the deep pineal of the rat (1980)
    Springer
    Cell & tissue research 210 (1980), S. 283-294 
    Details
    ISSN: 1432-0878
    Keywords: Deep pineal ; Pineal complex ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The results presented here reveal that in adult Sprague-Dawley and Wistar rats the pineal gland represents a complex rather than a single organ. Regularly one can distinguish (i) pineal tissue in the intercommissural region as a deep pineal, (ii) a superficial pineal, which represents the major part of the pineal complex, and (iii) nearly always a parenchymal stalk of variable length. The volume of the deep pineal with the adjacent parenchymal stalk exhibits great interindividual variation. It amounts to 127±39×105 μm3 (mean ±standard deviation). The histological appearance of the deep and superficial pineal tissue is fairly similar. The intrinsic cells of the deep and superficial pineal differ in nuances only. Karyometry reveals that the nuclear volumes of the intrinsic cells of the deep pineal are very variable ranging from 90–450 μm3, with a mean value of 207 μm3. The changes over a period of 24 h reach statistical significance.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00237616
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 82
    Electronic Resource
    Electronic Resource
    Investigations on the turnover of adrenocortical mitochondria (1980)
    Springer
    Cell & tissue research 210 (1980), S. 333-337 
    Details
    ISSN: 1432-0878
    Keywords: Zona glomerulosa ; Angiotensin II ; Mitochondria ; Stereology ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effects of a chronic treatment with angiotensin II (up to 15 consecutive days) on the mitochondria of the rat zona glomerulosa cells were investigated by electron microscopic and stereological methods. Angiotensin induced a significant increase in the volume of the mitochondrial compartment. Up to the 3rd day of treatment this was due only to the hypertrophy of the organelles, and from the 3rd to the 15th day exclusively to mitochondrial proliferation. The hypothesis that angiotensin controls the growth and proliferation of rat zona glomerulosa mitochondria is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00237620
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 83
    Electronic Resource
    Electronic Resource
    Circadian morphometric variation in gastric parietal cell populations of the rat (1980)
    Springer
    Cell & tissue research 211 (1980), S. 175-177 
    Details
    ISSN: 1432-0878
    Keywords: Parietal cell ; Stomach ; Circadian rhythm ; Morphometry ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Gastric parietal cells of rats maintained under standardized conditions and fed ad libitum were examined by electron microscopy at 6 time points of the 24-h day. Morphometric determinations were made on 4 cell characteristics. The volume density of secretory canaliculi was maximal at the mid-dark sampling point and decreased during the light phase; a secondary peak was seen 1 h before the onset of darkness. The surface density of microvesicles and RER fluctuated inversely with the pattern displayed by secretory canaliculi. The number of multivesicular bodies per cytoplasmic area exhibited a single peak, 1 h after the onset of darkness. It was further noted that parietal cells in the necks and bases of glands differed morphologically and that their organelle populations varied at individual circadian rates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233733
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 84
    Electronic Resource
    Electronic Resource
    Neurohormones in the intercellular clefts and in glia-like cells of the rat brain (1980)
    Springer
    Cell & tissue research 211 (1980), S. 251-268 
    Details
    ISSN: 1432-0878
    Keywords: Somatostatin ; Luliberin (LRF) ; Intercellular clefts (brain) ; Immunoreactive glia-like cells ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary With the aid of electron microscopic immunocytochemistry following the application of antisera against somatostatin and luliberin (LRF), a labeling of the intercellular clefts in different areas of the brain was observed. This labeling is especially conspicuous near the basal pole of the cuboidal ependymal cells, but is also generally present in all regions containing neurohormone-producing perikarya or their processes (for example, the preoptic area, the basal ganglia and the cortex). Furthermore, in all these regions displaying labeled intercellular clefts, glialike cells and sparsely ciliated ependymal cells are found, the secondary lysosomes of which exhibit an immunoreactivity resembling that observed in the intercellular clefts. As sources of the immunoreactive material the following possibilities are discussed: (i) perikarya producing somatostatin or LRF, situated in the wall of the third ventricle and sending fibers between the cuboidal ependymal cells, (ii) hypothalamic and extrahypothalamic projections of both peptidergic systems, and (iii) in the case of somatostatin, immunoreactive perikarya in the cortex.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00236448
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 85
    Electronic Resource
    Electronic Resource
    The adsorptive and transport capacity of tanycytes during the perinatal period of the rat (1980)
    Springer
    Cell & tissue research 211 (1980), S. 493-501 
    Details
    ISSN: 1432-0878
    Keywords: Hypothalamo-hypophysial system ; Rat ; Tanycytes ; Transport ; Ontogeny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Transport of ferritin and horseradish peroxidase from the 3rd ventricle to the median eminence was examined in rats during the perinatal life, the time when functional interrelations between hypothalamus and hypophysis are established. Protein tracers injected into the lateral ventricle are adsorbed on the apical surface of the tanycyte, mainly on its protrusions or in indentations. On the 18th day of prenatal life a few small bleblike protrusions are observed. After birth microvilli appear. In time their concentration increases to result in an increase of adsorbed substances. They are taken up by smooth and coated pinocytotic vesicles and transported to the basal portion of the cell or to the intercellular space bypassing junctional complexes. In addition to pinocytotic vesicles protein tracers fill channels of smooth ER or Golgi complex and multivesicular bodies illustrating a process probably involved in metabolic or secretory processes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234403
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 86
    Electronic Resource
    Electronic Resource
    Localization and neurophysiological properties of motoneurones of the M. triceps surae of the rat after retrograde labelling with evans blue (1980)
    Springer
    Cell & tissue research 212 (1980), S. 73-81 
    Details
    ISSN: 1432-0878
    Keywords: Motoneurones ; Triceps surae muscle ; Retrograde labelling (Evans blue) ; Excitability ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Evans blue was injected into either the medial and lateral gastrocnemius or the soleus muscle of adult albino rats to mark retrogradely the corresponding motoneurones in the spinal cord. The labelled motoneurones were identified by the red fluorescence of their perikarya in the ventral horn of segments L4–L6. In addition, a monosynaptic reflex action potential was recorded only in the ventral roots L4–L6 after stimulation of the nerves to the medial and lateral gastrocnemius and the soleus muscle. Excitability and reflex latencies of labelled and unlabelled motoneurones of segments L4–L6 as well as the conduction velocities of their axons were measured. Apart from a small but statistically nonsignificant increase in excitability, no functional differences were found between labelled and control neurones. Thus, retrograde labelling of motoneurones with Evans blue prior to performing electrophysiological experiments has the advantage that the marked motoneurones can be identified under the fluorescence microscope without the need of additional staining or fixation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234034
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 87
    Electronic Resource
    Electronic Resource
    Functional difference between “classical” neurosecretory material and vasopressin-like substances of the outer layer of the median eminence (1980)
    Springer
    Cell & tissue research 212 (1980), S. 257-277 
    Details
    ISSN: 1432-0878
    Keywords: CRF ; Vasopressin ; Adrenalectomy ; Rat ; Histology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In female Wistar rats the influence of adrenalectomy and NaCl administration on the amount of “classical” neurosecretory material (cNSM) in the supraoptico-hypophysial system and on vasopressin-like substance-containing granules (vlG) in the outer layer of the median eminence has been studied. In conjunction with appropriate sodium replacement, adrenalectomy induces an increase in the amount of vlG but does not alter the amount of cNSM. Administration of hypertonic saline diminishes cNSM but has no or only little influence on the amount of vlG. From the findings it is concluded that cNSM and vlG, in spite of their identical histochemical and immunohistochemical properties, have different functions. The functional significance of the vlG is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233960
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 88
    Electronic Resource
    Electronic Resource
    Two types of luliberin-immunoreactive perikarya in the preoptic area of the rat (1980)
    Springer
    Cell & tissue research 212 (1980), S. 443-455 
    Details
    ISSN: 1432-0878
    Keywords: LRF-immunoreactive perikarya ; Preoptic area ; Light and electron microscopic immunocytochemistry ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary At the light microscopic level, following immunostaining with a single antiserum against luliberin (LRF), two types of hormone-producing perikarya in the preoptic area are demonstrated. The two cell types differ in their morphological features: a bipolar, smooth-contoured cell type can be differentiated from an irregularly contoured unipolar type. Intermediate forms between both cell types occurring in the same area are not observed. Electron microscopically, both cell types contain labeled granules of similar size and immunoreactivity. It is dicussed whether the uneven surface of the one cell type is due to areas of synaptic contacts, and whether both cell types are integrated in different neuronal and functional circuits. Moreover, at the ultrastructural level, from the irregularly contoured LRF-producing perikarya a further positively stained cell type, probably a glial cell, can be differentiated. The specific labeling of the latter is caused by its content of immunoreactive lysosomal bodies. Differentiation between the labeled glial cells and the irregularly contoured LRF-producing perikarya is not possible at the light microscopic level.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00236509
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 89
    Electronic Resource
    Electronic Resource
    Differing immunoreactivities of somatostatin in the cortex and the hypothalamus of the rat (1980)
    Springer
    Cell & tissue research 212 (1980), S. 457-464 
    Details
    ISSN: 1432-0878
    Keywords: Somatostatin immunoreactivity ; Cortex ; Hypothalamus ; Light and electron microscopic immunocytochemistry ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Using an antibody against somatostatin (antiserum “F”), two somatostatin-immunoreactive systems, (i) a hypothalamic and (ii) an extrahypothalamic cortical system, are demonstrated in the rat. Another antiserum raised against somatostatin (antiserum “BS 102”) stains only the axons but not the perikarya of the hypothalamic system; the cortical somatostatin system does not react with this antiserum. The electron microscopic findings do not allow decision whether the above-mentioned hypothalamic and cortical neurons possess a common prohormonal form of somatostatin, immunoreactive only with antiserum “F”. They show, however, that the granules in both neuronal systems differ considerably; in the cortical neurons they measure approximately 65 nm in diameter, in the hypothalamic neurons 90–120 nm in diameter. Thus, both somatostatin systems are different and independent from one another.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00236510
    Location Call Number Expected Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...