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Ribosylzeatin and zeatin in tobacco crown gall tissue

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Abstract

Cytokinins were extracted from two cultures of tobacco crown gall tumor tissue: an unorganized tissue and a teratoma which produced leafy shoots. On Sephadex LH-20 column chromatography, extracts of both types of tissue yielded two peaks of cytokinin activity with elution volumes similar to ribosylzeatin and zeatin. Ribosylzeatin and zeatin were detected and quantified by coupled gas chromatography — mass spectrometry selected ion monitoring (GC/MS SIM), comparable quantities being found in the two extracts. Full mass spectral evidence for the presence of ribosylzeatin in both tissues was obtained. No evidence was found for the presence of N6-(Δ2-isopentenyl)adenosine (i6Ade) or N6-(Δ2-isopentenyl)adenine (i6Ade) although these compounds have been reported to occur in cytokinin-habituated tobacco callus tissues.

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Abbreviations

BAP:

6-benzylaminopurine

GC:

gas chromatography

GC/MS:

coupled gas chromatography-mass spectrometry

i6 Ade:

N6-(Δ2-isopentenyl)adenine

i6 Ado:

N6-(Δ2-isopentenyl)adenosine

RFE:

rotary film evaporation

SIM:

selected ion monitoring

TLC:

thin-layer chromatography

TMS:

trimethylsilyl

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Author notes

  1. I. M. Scott

    Present address: Department of Botany and Microbiology, University College of Wales, SY23 3DA, Aberystwyth, UK

Authors and Affiliations

  1. John Innes Institute, Norwich, UK

    I. M. Scott & G. Browning

  2. A.R.C. Food Research Institute, Norwich, UK

    J. Eagles

Authors
  1. I. M. Scott
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  2. G. Browning
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  3. J. Eagles
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Scott, I.M., Browning, G. & Eagles, J. Ribosylzeatin and zeatin in tobacco crown gall tissue. Planta 147, 269–273 (1980). https://doi.org/10.1007/BF00379831

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  • DOI: https://doi.org/10.1007/BF00379831

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