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  • Calcium  (21)
  • Development  (17)
  • Springer  (38)
  • 2015-2019
  • 1985-1989
  • 1980-1984  (38)
  • 1980  (38)
Collection
Keywords
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  • Springer  (38)
Years
  • 2015-2019
  • 1985-1989
  • 1980-1984  (38)
Year
  • 1
    Electronic Resource
    Electronic Resource
    In vitro effects of vitamin D3 metabolites on rat calvaria cAMP content (1980)
    Springer
    Calcified tissue international 30 (1980), S. 209-216 
    Details
    ISSN: 1432-0827
    Keywords: Calvarium ; cAMP ; Vitamin D3 metabolites ; Calcium ; Parathyroid hormone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Results from in vitro works suggest that 1,25- and 24,25-dihydroxyvitamin D3 (1,25-(OH)2D3 and 24,25-(OH)2D3) act on bone via different mechanisms. The present investigation was performed to study the effect of these two metabolites and of their precursor 25-hyxdroxyvitamin D3 (25-(OH)D3) on bone cAMP content in vitro. Rats' paired half calvaria were incubated under sterile conditions with one vitamin D3 derivative (10−13 to 10−9 M) or with ethanol (0.005 ml for 15 min to 24 h in 1 ml medium containing 0, 0.2, 1, 2, or 3 mM calcium. In some experiments: (a) cycloheximide (10−5M) was added simultaneously with the vitamin D3 metabolites; (b) 1–84 bPTH (5 × 10−8 M) was added for 5 or 15 min at the end of the 24 h incubation. Calvaria were immersed in 1 ml TCA 5% 4°C and homogenized. The cAMP was extracted with diethylether and measured by a competitive protein binding assay. Results bring further evidence for a particular effect of low doses of 24,25-(OH)2D3 (10−9 to 10−12M) and of 25-(OH)D3 (10−9 to 10−11M) on bone, different from that of 1,25-(OH)2D3: cAMP content was higher in 24,25-(OH)2D3- or 25-(OH)D3-treated and lower in 1,25-(OH)2D3-treated calvaria than in ethanol-treated ones with 1 mM calcium. The 1,25-(OH)2D3 effect persisted in calcium-free medium whereas 25-(OH)D3 and 24,25-(OH)2D3 effects could not be observed with 0 mM nor with 3 mM calcium. The required duration of the preincubation (over 1 h) as well as the inhibitory action of cycloheximide may suggest an involvement of protein synthesis in the vitamin D3 metabolites effects. Neither 1,25-(OH)2D3 nor 24,25-(OH)2D3 affected the PTH-induced increase in bone cAMP content.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02408630
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  • 2
    Electronic Resource
    Electronic Resource
    Effects of magnesium ion on parathyroid hormone secretion in vitro (1980)
    Springer
    Calcified tissue international 32 (1980), S. 201-206 
    Details
    ISSN: 1432-0827
    Keywords: Magnesium ; Parathyroid hormone ; Secretion ; In vitro ; Calcium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary In a well-defined in vitro perifusion system, the effects of extracellular magnesium concentration (Mg) on parathyroid hormone (PTH) secretion by bovine parathyroid tissue were examined. At Mg less than 0.8 mM, the ability of the glands to secrete hormone maximally in response to low calcium (Ca) stimulation was progressively impaired. Low Mg also impaired the ability of isoproterenol, dibutyryl cyclic AMP and theophylline to stimulate hormone release. The defect in hormone release at low Mg observed in vitro was analogous to the well-documented inhibition of secretion observed in vivo. Increases in Mg from 0 to 0.8 mM rapidly repaired the defect in hormone secretion. At Mg above 1.0 mM there was a Ca-like effect on hormone release, with a progressive decrease in secretion at increased Mg. Although its mechanism is not yet clear, the low Mg effect appears to impair principally the process of hormone release rather than its biosynthesis or storage.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02408542
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  • 3
    Electronic Resource
    Electronic Resource
    The effect of temperature onshibire ts cell clones in the compound eye ofDrosophila melanogaster (1980)
    Springer
    Development genes and evolution 188 (1980), S. 55-63 
    Details
    ISSN: 1432-041X
    Keywords: Drosophila ; Compound eye ; shibire ts ; Development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have analysed the effect of temperature on both developing and adult eye cell clones homozygous forshi ST139, a temperature-sensitive mutant ofDrosophila melanogaster. The mutant gene, autonomous in its cellular expression, causes structural modifications of ommatidial cells when adult clones of cells are exposed to the restrictive temperature (29°C) for several days. However, the mutant phenotype reverses to normal within 4 days at the permissive temperature (20°C). The results of pulse, shift-up and shift-down experiments show that the temperaturesensitive period for developing compound eye cells is from the late second instar up to the early pupa. Cytodifferentiation of compound eye cells is blocked by restrictive temperature treatment during this period, whereas cell proliferation does not seem to be directly affected. These results are discussed with regard to the other known aspects of the phenotype observed in mutant individuals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00848610
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  • 4
    Electronic Resource
    Electronic Resource
    Ganglioside changes in the chicken optic lobes and cerebrum during embryonic development (1980)
    Springer
    Development genes and evolution 188 (1980), S. 205-213 
    Details
    ISSN: 1432-041X
    Keywords: Gangliosides ; Development ; Brain ; Chicken
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The developmental profiles of 15 different gangliosides of the optic lobes and cerebrum of the chicken were followed from the 6 th day of incubation to hatching and correlated to morphological development. Five of these gangliosides appearing in both structures between the sixth and tenth day, have not been reported previously in higher vertebrates. Three chromatographed on TLC-plates similarly to GT3, GT2, and GT1c gangliosides, which have been demonstrated in fish brain. One fraction moved just below GQ1b and is suggested, to contain GQ1c. These “novel” gangliosides, which are possibly related to a recently proposed separate and probably phylogenetically older biosynthetic pathway, contained up to 20% of total ganglioside sialic acid. The fifth “novel” fraction, containing up to 16% of total ganglioside-sialic acid, moved below the penta-sialoganglioside GP1 and is suggested to contain hexa-sialogangliosides. There were two main changes in ganglioside synthesis, which were identical in both structures. The first occurred from the sixth to the eleventh day, parallel to decreased proliferation, maximal cell migration and neuroblast differentiation, GD3 and GD2 decreased rapidly in favour of GQ1b, GP1, and to the “novel” fractions, described above. The second occurred from the eleventh to the eighteenth day, parallel to increased growth and arborization of dendrites and axons as well as functional establishment of synaptic contacts, there was a sharp rise in the amount of GD1b, GT1b, and GD1a. Concomitantly the “novel” gangliosides decreased. At hatching GD1a was the predominant ganglioside. GM3, GM2, and GM1 were always minor fractions, each accounting for less than 4% of total ganglioside-sialic acid. GM4 was never detected, indicating neglegible myelinisation until hatching.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00849050
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  • 5
    Electronic Resource
    Electronic Resource
    Studies on the regulation of the branched chain amino acyl-tRNA synthetases of the fungusNeurospora crassa (1980)
    Springer
    Development genes and evolution 189 (1980), S. 171-180 
    Details
    ISSN: 1432-041X
    Keywords: Amino acyl-tRNA synthetases ; Development ; Nuclear-mitochondrial interactions ; Neurospora
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The specific activities of the branched chain amino acyl-tRNA synthetases from the cytosolic and mitochondrial fractions ofN. crassa were low in dormant conidia and increased during germination, reaching a maximum 8 h after inoculation. This stage of development is characterised by high rates of many other cellular activities. The increases in activity of synthetases of both cytosol and mitochondria are inhibited by cycloheximide indicating that they are synthesized on cytoplasmic ribosomes. The mitochondrial synthetases show a stimulation of their specific activity when mitochondrial RNA and protein synthesis are inhibited by either ethidium bromide or chloramphenicol suggesting that a mitochondrial translation product regulates the synthesis of the mitochondrial synthetases. The activities of amino acyl-tRNA synthetases are dependent on energy production. When respiration is uncoupled from oxidative phosphorylation, synthetase specific activities decrease although the activities of other mitochondrial enzymes like NADH-dehydrogenase increase. This phenomenon suggests that more than one mechanism regulates the synthesis of mitochondrial proteins which are formed on cytoplasmic ribosomes. The synthesis of branched chain amino acyl-tRNA synthetases ofNeurospora is neither repressed by their cognate amino acids, nor is there inhibition by the precursors of these amino acids, as has been observed in other amino acyl-tRNA synthetases of various organism includingNeurospora.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00868675
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  • 6
    Electronic Resource
    Electronic Resource
    Blood/bone disequilibrium: IV. Reciprocal effects of calcium and phosphate concentrations on ion fluxes (1980)
    Springer
    Calcified tissue international 32 (1980), S. 229-236 
    Details
    ISSN: 1432-0827
    Keywords: Bone ; Ion influxes ; Calcium ; Phosphate ; Exchange
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Quantitative measurements were made of the ion fluxes of calcium and phosphate into and from calvaria (mouse or rat) when clamped in specially designed micro-Ussing chambers. The effects of varying concentrations of calcium were examined on the influx and efflux of calcium and of its counterion, phosphate. A comparable series of experiments was performed with varying phosphate concentrations. Both ions, as their concentrations increased, depressed their own influx, increased their own efflux, and significantly increased the equilibrium concentration, E/K, supported by the calvaria. Similarly, both ions, as their concentrations increased, affected the influx or efflux of their counterion only slightly but did depress the counterion's equilibrium level, E/K, significantly. In spite of these changes it was shown that calvaria effectively buffered the medium at physiological concentrations of calcium and phosphate. The buffering capacity, however, was small, and the balance, E/K, was modified by small uptake or loss of either ion. The small size of the interacting mineral pool was confirmed by direct measurement of the rapidly exchanging fractions of both calcium or phosphate. They were only ∼1% of the total ions present. The significance of these findings is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02408546
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  • 7
    Electronic Resource
    Electronic Resource
    Effects of a bone resorptive factor from human cancer ascites fluid on rat bone cell calcium and cyclic AMP (1980)
    Springer
    Calcified tissue international 30 (1980), S. 191-197 
    Details
    ISSN: 1432-0827
    Keywords: Bone cells ; Cyclic AMP ; Calcium ; Ascites fluid resorptive protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The effects of a bone resorptive protein isolated from human cancer ascites fluid on bone cell calcium and cyclic AMP were studied with fetal rat cells. The osteoclast-activating factor increased bone cell calcium uptake at 37°C and 4°C with no direct effects on calcium efflux. Concentrations of the resorptive factor that increased in vitro bone resorption and cell calcium uptake had no effect on cyclic AMP. The effects of the protein on calcium uptake were not specific for bone cells, and large increases were also observed in isolated fetal rat skin cells. These studies suggest that increases in the permeability of the cell membrane to calcium are involved in the mechanism of action of the ascites fluid resorptive protein.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02408627
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  • 8
    Electronic Resource
    Electronic Resource
    Effects of pyrophosphate and diphosphonates on the dissolution of hydroxyapatites using a flow system (1980)
    Springer
    Calcified tissue international 31 (1980), S. 153-159 
    Details
    ISSN: 1432-0827
    Keywords: Hydroxyapatite ; Dissolution ; Pyrophosphate, Diphosphonates ; Calcium ; Phosphate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Pyrophosphate and diphosphonate ions have been said to diminish the dissolution of hydroxyapatite crystals, because they lower the equilibrium concentrations of calcium and phosphate ions in the bulk solution around hydroxyapatite crystals in a closed system. However, in a closed system these effects are not necessarily due to an effect on dissolution alone. In this paper we have used a continuous flow system to study the effects of pyrophosphate and two diphosphonates, ethane-1-hydroxy-1,1-diphosphonate and dichloromethane diphosphonate, on the dissolution of hydroxyapatite. All three compounds decreased markedly the rate of dissolution of hydroxyapatite as well as the exchangeable pools of calcium and phosphate ions around the cystals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02407176
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  • 9
    Electronic Resource
    Electronic Resource
    Calcium homeostasis and bone pathology in magnesium deficient rats (1980)
    Springer
    Calcified tissue international 31 (1980), S. 231-238 
    Details
    ISSN: 1432-0827
    Keywords: Magnesium ; Bone ; Calcium ; Parathyroid gland
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary Calcium homeostasis and bone pathology were studied in weanling rats fed a low (70 ppm) magnesium diet for 2–21 days. The rats developed significant, progressive hypercalcemia after 6 days on the diet. The increase in blood calcium was accompanied by progressive hypoactivity of the parathyroid gland (PTG), as determined by histologic and morphometric analyses. Thus hyperactivity of the PTG could not have been responsible for the hypercalcemia observed. Histologic examination of femora and humeri from magnesium-deficient rats showed progressive subperiosteal hyperplasia, consisting of undifferentiated osteoprogenitor cells and fibrous tissue, after 7 days of deficiency. The presence of unmineralized osteoid tissue in the metaphyses indicated that mineralization was not proceeding normally. The alterations in differentiation of osteoprogenitor cells, together with the failure of mineralization, resulted in significantly lower rates of bone formation (as measured by fluorochrome labeling) in the magnesium-deficient rats. Basophilic cementing lines and inactive osteocytes in the cortices of bones from magnesium-deficient rats indicated that bone resorption was also severely reduced in magnesium deficiency. We postulate that bone magnesium depletion (66% by day 21) has a direct negative effect on osteoblastic and osteocytic activity, and may explain, in part, the decreased responsiveness of bone to parathyroid hormone (PTH) that has been observed in magnesium-deficient animals.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02407186
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  • 10
    Electronic Resource
    Electronic Resource
    Rhythmic dentinogenesis in the rabbit incisor: Allometric aspects (1980)
    Springer
    Calcified tissue international 32 (1980), S. 45-53 
    Details
    ISSN: 1432-0827
    Keywords: Dentin ; Periodicity ; Allometry ; Calcium ; Sulfur
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary We have described differences in the aspects of biological rhythms for calcium and sulfur deposition on the labial and lingual sides of the growing rabbit incisor, where growth occurs along a spiral axis. The calcium oscillations appear to be smoother on the labial side than on the lingual side. The lingual side is characterized by high-frequency rhythms with high amplitudes which possess the greatest percent of the power (Fourier analysis). These observations also reflect a difference in behavior of the mean Ca concentration across the labial and lingual sides. Sulfur rhythms on the labial side have higher amplitudes than those on the lingual side, but systematic differences in distribution of power between high and low frequencies is not as pronounced as in the case of Ca. The differences in Ca rhythms reflect differences in the growth rates of incisors on either side of the spiral axis. The labial side grows slightly faster than the lingual side, and its odontoblasts secrete Ca along the spiral axis and toward the pulp cavity at the same time. Thus the resultant direction of growth is more nearly opposite the extension of the occlusal end on the labial side, and Ca is consequently deposited over a wider area relative to that on the lingual surfaces. On the lingual side, Ca is deposited within a more limited area, and growth must therefore be continuous at high frequencies. The distribution of Ca on both sides of the tooth reflects these differences in growth rate and periodicity in two ways. First, given a unit area of tooth, the calcium concentration on the labial side is less than that of the lingual side. Second, whereas the calcium concentration on the labial side declines rapidly from the enamel-dentin junction to the pulp cavity, it is uniformly high across the lingual side because its growth is more continuous at high frequencies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02408520
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  • 11
    Electronic Resource
    Electronic Resource
    Effects of 1α-hydroxy vitamin D3 on the rachitic chick intestine: A comparison to the effects of 1,25-dihydroxyvitamin D3 (1980)
    Springer
    Calcified tissue international 32 (1980), S. 9-17 
    Details
    ISSN: 1432-0827
    Keywords: 1α-Hydroxy vitamin D3 ; 1,25-Dihydroxyvitamin D3 ; Calcium ; Transport ; Intestine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The timed sequence of events following the oral administration of 1α-hydroxy vitamin D3 (1αOHD3) to rachitic chicks was compared to that following a comparable dose of 1α,25-dihydroxyvitamin D3 (1,25(OH)2D3). RNA polymerase activity was maximally increased 20% by 1αOHD3 within 1 to 2 h and returned to control values after 8 h. Alkaline phosphatase activity was stimulated by 4 h and was maximal (3- to 5-fold increase) at 24 h. Calcium binding protein (CaBP) was detected initially within epithelial cells at the proximal end of the villus (just above the crypt) 6 to 8 h after 1αOHD3 administration, in epithelial cells lining the proximal half of the villus by 24 h, and in epithelial cells along nearly the entire villus by 48 h. At no time did goblet cells contain CaBP. Serum calcium concentrations were significantly elevated in 2 h and maximal by 12 h (an increase of 3.6 mg/dl). Calcium accumulation by the intestinal mucosa in vitro was increased by 6 to 8 h and maximal (60% increase over controls) at 24 h. Phosphate accumulation by the intestinal mucosa in vitro was increased by 6 h and maximal (105% increase over controls) between 8 and 24 h. 1,25(OH)2D3 increased CaBP and calcium accumulation by 4 h, 2 h sooner than did 1αOHD3. 1,25(OH)2D3 decreased serum calcium levels and increased serum phosphate levels at 2 h unlike 1αOHD3. No difference in the effects of these compounds on alkaline phosphatase activity, RNA polymerase activity, and phosphate accumulation could be demonstrated. These results are consistent with the possibility that 1αOHD3 may not require conversion to 1,25(OH)2D3 for all of its biological effects.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02408517
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  • 12
    Electronic Resource
    Electronic Resource
    In vitro perifusion for the study of parathyroid hormone secretion: Effects of extracellular calcium concentration and beta-adrenergic regulation on bovine parathyroid hormone secretion in vitro (1980)
    Springer
    Calcified tissue international 32 (1980), S. 19-27 
    Details
    ISSN: 1432-0827
    Keywords: Perifusion ; Parathyroid hormone ; In vitro ; Calcium ; Beta regulation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary An in vitro perifusion system was used to study parathyroid hormone (PTH) secretion in response to calcium (Ca) and beta-adrenergic agents. Perifused parathyroid tissue responded to changes in Ca within the physiologic range during experiments up to 5 h. There was rapid secretory stimulation after exposure to low Ca, with the maximum response being observed at 20 min. Normal bovine glands showed a Ca-independent nonsuppressible component of PTH release at concentrations of Ca above physiologic. 1-isoproterenol produced rapid stimulation of PTH release, the response being blocked by a beta antagonist. The maximum secretory response to either low Ca (0.5 mM) or 1-isoproterenol (10−5 M) was enhanced when the two stimuli were applied simultaneously. The response to isoproterenol was blocked by raising Ca to 2.5 mM. Although d,l-propranolol (10−4 M) caused mild suppression of PTH release at a Ca of 1.25 mM, it did not cause additional suppression at 2.5 mM Ca nor did it decrease the response to 0.5 mM Ca stimulation. The secretory response of the gland to low Ca was sustained at a level more than double the baseline rate. The response to isoproterenol was more transient, with a return to or toward baseline secretion within 60 min. These results suggest that beta agonists and low Ca have separate but related mechanisms for stimulating PTH release and may affect different pools of hormone. The perifusion system described is a relatively simple technique for assessing the kinetics and interactions of various stimulators of PTH secretion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02408518
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  • 13
    Electronic Resource
    Electronic Resource
    Rhythmic dentinogenesis in the rabbit incisor: Circadian, ultradian, and infradian periods (1980)
    Springer
    Calcified tissue international 32 (1980), S. 29-44 
    Details
    ISSN: 1432-0827
    Keywords: Rabbit ; Dentin ; Calcium ; Sulfur ; Periodicity ; Circadian ; Ultradian
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary We have identified a variety of biological rhythms involved in the apposition and mineralization of dentin in the rabbit incisor. Animals were injected during the day or night with lead acetate at 2-week intervals—to provide biological time markers in forming dentin—and transverse undecalcified sections of the lower incisors were prepared for electron microprobe analysis. The positions of the lead markers were identified, and the continuous distribution of calcium and sulfur was measured at 1 µm intervals between the markers. In thin sections stained with hematoxylin after decalcification, the widths of a series of structural increments (bands) were measured with an ocular micrometer. Fourier analysis of the data revealed spectra of structural and compositional rhythms with a range of periodicities which extended from a matter of hours [ultradian (〈24 h)] to days [infradian (〉24 h) and circadian (approximately 24 h)]. The structural and compositional rhythms appeared to be independent to the extent that they did not necessarily have the same periods, or amplitudes. Nor were there simple phase relationships between all of the rhythms. At some times, Ca and S fluctuations are inversely proportional (180° out of phase), but in other cases they are directly proportional or out of phase by varying degrees other than 180°. The analyses thus suggest that calcium and sulfur deposition (representing mineral and glycosaminoglycan deposition, respectively) are not simply inversely proportional, and that the hematoxylin-stained structural increments did not solely reflect differences in the distribution of the mineral components in dentin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02408519
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  • 14
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    Electronic Resource
    Ultrastructural localization of calcium in the mandibular condylar growth cartilage of the rat (1980)
    Springer
    Calcified tissue international 30 (1980), S. 27-34 
    Details
    ISSN: 1432-0827
    Keywords: Ultrastructure ; Calcium ; Cartilage ; Vesicles
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine , Physics
    Notes: Summary The potassium pyroantimonate technique was utilized for the selective subcellular localization of calcium in the mandibular condylar cartilage of 1-day-old rats. Electron dense calcium pyroantimonate precipitates were localized principally in mitochondria and at the cell membrane of the chondrocytes. In addition, small intracellular vesicles 0.1–0.2µm in diameter were observed in proximity to the cell membrane of chondrocytes of the mid-hypertrophic zone. The results suggest that these vesicles were being extruded from the cell into the extracellular matrix. Energy-dispersive analysis by X-rays confirmed that calcium is the principal cation of the electron-dense precipitates.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02408603
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  • 15
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    Using ecological knowledge for development planning (1980)
    Springer
    Environmental management 4 (1980), S. 13-20 
    Details
    ISSN: 1432-1009
    Keywords: Planning ; Management ; Natural resource planning ; Development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Since October 1977, the East-West Environment and Policy Institute in Honolulu has been conducting a multinational collaborative project to enhance the preparation and utilization of natural systems assessments in developing countries. This paper presents some of the findings to date: 1. Channels are developing rapidly for transferring ecological knowledge into political and administrative decision making. 2. The systematic approach of ecology is replacing “environmental quality” as the organizing concept for information about natural resources and the environment. 3. Benefit-cost analysis is a promising method for integrating ecological knowledge into economic development decision making. 4. The lack of baseline information, inventories, and predictive capability will not be remedied soon or easily; thus priorities for ecological research are essential.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01866216
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  • 16
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    Changes of the cytoplasmic ultrastructure during development of sclerotia in Claviceps purpurea (Fr.) Tul. (1980)
    Springer
    Archives of microbiology 125 (1980), S. 251-257 
    Details
    ISSN: 1432-072X
    Keywords: Claviceps purpurea ; Ultrastructure ; Development ; Sclerotium ; Oleosomes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The development of sclerotia of Claviceps purpurea was investigated by light and electron microscopy. During the first days after infection sterigma and conidiospores are formed. The spores show a moderately developed vacuolar system, they are thick walled and contain about 20% lipid (related to the cell volume) embedded in glycogen. The sterigma are cylindrical unicellular hyphae with electron dense cytoplasm and isolated strongly contrasted lipid droplets. In maturing sclerotia the hyphae become septated with increasingly thick cell walls and a large lipid content. The lipid forms small droplets in young cells, while in the mature sclerotium it occurs in the form of very large drops, occupying the major part of the cell. Simultaneously the composition of the lipid is changed. The mature cells have several nuclei. They are partially connected by osmiophilic substances, forming a network of intercellular spaces.
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    URL: http://dx.doi.org/10.1007/BF00446885
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  • 17
    Electronic Resource
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    Prenatal and early postnatal development of the glial cells in the median eminence of the rat (1980)
    Springer
    Cell & tissue research 211 (1980), S. 117-137 
    Details
    ISSN: 1432-0878
    Keywords: Eminentia mediana ; Neurohypophysis ; Development ; Pituicytes ; Tanycytes ; Astrocytic tanycytes ; Oligodendrocytes ; Astrocytes ; Microglia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The development of the glial cells of the rat median eminence (ME), including the supraependymal cells, was investigated from embryonic day (ED) 14 through postnatal day (PD) 7, and pituicyte development from ED 12 through ED 17. The anlage of the ME and neurohypophysis shows a neuroepithelial-like structure at ED 12. From ED 13 to 15, the cells of both regions start to differentiate. At the ultrastructural level, only one cell type appears. At the beginning of ED 16, glioblasts of the oligodendrocyte and astrocyte series migrate laterally (from the region of the arcuate nucleus) into the ME. Also at this time the first distinctive structural features appear in the neurohypophysial anlage, the cells of which later develop into pituicytes. Starting at ED 18, tanycytes and astrocytic tanycytes arise in the ME from local glial cells, and somewhat later oligodendroblasts and astroblasts are formed from immigrant glioblasts. Due to their common features, the pituicytes, tanycytes and astrocytic tanycytes apparently represent different forms of the same parent cell type. Microglial and supraependymal cells are first seen at ED 12. Initially, they resemble the prenatal phagocytic connective tissue cells and mature in the fetus into typical electron-dense microglia and macrophage-like supraependymal cells. Both cell types are apparently of mesodermal origin. The microglial elements of the ME probably migrate from the mesenchyma through the basement into the nervous tissue. The intraventricular macrophages of the infundibular region may originate from microglia, epiplexal cells and subarachnoid macrophages.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00233728
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  • 18
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    Postnatal development of somatotrophs and mammotrophs in the pars distalis of the C57BL mouse (1980)
    Springer
    Cell & tissue research 211 (1980), S. 441-448 
    Details
    ISSN: 1432-0878
    Keywords: Somatotroph ; Mammotroph ; Development ; Mouse ; Pituitary
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The pars distalis of the pituitary gland of the C57BL mouse was studied by means of electron microscopy during postnatal stages of males ranging in age from newborn through 24 days, with particular emphasis on the somatotrophs and mammotrophs. During this period, growth curves were plotted in order to correlate postnatal growth patterns with the state of differentiation of the somatotrophs in the pars distalis. In the newborn, the somatotrophs show well developed organelles, including rough endoplasmic reticulum and Golgi complexes. These cells are not as densely packed with granules as the adult somatotrophs; however, from days 5 through 24, they show a progressive accumulation of granules. Although mammotrophs are scarce in the newborn, they are readily distinguishable in the pars distalis at 5 days. Male mice in small litters show a progressive increase in body weight between birth and 11–12 days, at which time the rate slackens until 18–19 days when the rate again increases. Growth curves for mice from large litters are similar to those from smaller litters, except that the transitory decrease in rate is more prominent.
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    URL: http://dx.doi.org/10.1007/BF00234398
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    Role of innervation on the embryonic development of skeletal muscle (1980)
    Springer
    Cell & tissue research 210 (1980), S. 383-393 
    Details
    ISSN: 1432-0878
    Keywords: Avian skeletal muscle ; Development ; Normal, aneural, paralyzed muscle
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The extent to which the motor innervation regulates the embryonic development of skeletal muscle was investigated by comparing changes in normal, aneural, and paralyzed superior oblique muscle of the duck embryo. The muscle was made aneural by permanently destroying the trochlear motor neurons with electrocautery on day 7 i.e., three days prior to innervation. Embryos were paralyzed by daily application of α-bungarotoxin onto the chorioallantoic membrane from day 10 onwards. The differentiation of myoblasts and myotubes in the aneural muscle was severely affected and did not progress to the myofiber stage. A mass of dead cells in the aneural muscle was replaced by connective tissue. Although the differentiation of myoblasts and myotubes was also retarded in the paralyzed muscle, numerous muscle cells progressed to the myofiber stage. Neuromuscular junctions of normal ultrastructure were seen in all paralyzed muscles. Degeneration of some cells in the paralyzed muscle occurred but there was no evidence of a massive wave of cell death similar to that observed in the aneural muscle. These observations suggest that both the trophic factors from the nerve and the nerve-evoked muscle activity are essential for the execution of the developmental program of the muscle. Trophic factors may play a larger role in differentiation, and maintenance of the muscle than muscle activity.
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    URL: http://dx.doi.org/10.1007/BF00220196
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    Studies on formation and resorption of fish scales (1980)
    Springer
    Cell & tissue research 211 (1980), S. 303-316 
    Details
    ISSN: 1432-0878
    Keywords: Fish scale ; Fine structure ; Development ; Calcification ; Cyprinodon variegatus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Scale formation in Cyprinodon variegatus was found to be initiated at about 26 to 30 days after hatching. Ultrastructural investigation revealed that within 4 to 6 h in the first-formed scales the marginal cells begin to flatten and differentiate into osteogenic cells, which later change to osteoblasts and fibroblasts. These cells are separated from the surrounding epithelial cells by a basal lamina. The osteoid is formed by the marginal and osteogenic cells; the osseous layer by the osteoblasts; and the fibrillary plate by the fibroblasts. The osteoid is formed within 2 to 3 h after the initiation of the scale, and within 20 to 24 h the osseous layer is formed. Hydroxyapatite crystals are deposited in the matrix of the osseous layer without apparent association with collagen fibers. No matrix vesicles or dense bodies are evident at the sites of calcification. The fibrillary plate arises 18 to 20 h after the initiation of the scale. It is also partially calcified, but not before the third week of scale formation. The crystals develop almost exclusively between the collagen fibers at the extreme edge of the calcifying front, but solid calcification of the fibers results with further growth of the crystals. The fibroblasts appear to participate in calcification of the fibrillary plate.
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    URL: http://dx.doi.org/10.1007/BF00236451
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    The parcellation theory and its relation to interspecific variability in brain organization, evolutionary and ontogenetic development, and neuronal plasticity (1980)
    Springer
    Cell & tissue research 213 (1980), S. 179-212 
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    ISSN: 1432-0878
    Keywords: Neocortex ; Evolution ; Development ; Plasticity ; Visual system
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Recently discovered neocortical equivalents in anamniotes and certain patterns of interspecific variability in brain organization provide new insights into evolutionary and ontogenetic mechanisms of development. The new data suggest that nervous systems become more complex, not by one system invading another, but by a process of parcellation that involves the selective loss of connections of the newly formed daughter aggregates and subsystems. The parcellation process is reflected in the normal ontogenetic development of the CNS in a given species and can be manipulated, to a certain extent, by deprivation or surgically induced sprouting. The parcellation theory allows certain predictions about the range of variation of a given system at all levels of analysis including the cellular and aggregate levels. For example, the interspecific variability in organization of cortical columns, thalamic nuclei, cortical areas and tectal layers can be explained. The findings, summarized here, suggest that diffuse, undifferentiated systems existed in the beginning of vertebrate evolution and that during the evolution of complex behaviors, and analytical capacities related to these behaviors, a range of patterns of neural systems evolved that relate to these functions. One principle underlying the growth, differentiation and multiplication of neural systems appears to be the process of parcellation as defined by the theory.
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    URL: http://dx.doi.org/10.1007/BF00234781
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    Microtubules, dendritic spines and spine apparatuses (1980)
    Springer
    Cell & tissue research 208 (1980), S. 171-181 
    Details
    ISSN: 1432-0878
    Keywords: Microtubules ; Dendritic spine apparatus ; Synapse ; Development ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Using techniques for enhanced microtubular preservation, including albumin pretreatment (Gray, 1975), occipital cortex of rats was studied electron microscopically at various ages of development. A close structural relationship was seen between microtubules, sacs of SER and the postsynaptic “thickening” in primordial spines and with the dense “plate” material of spine apparatuses. Stereoscopic preparations in addition show a more complicated substructure than previously described for the “plate”. Microtubules may contribute to the formation of the “plate” of the spine apparatus which in turn is associated with the postsynaptic “thickening” of the mature spine. Possible functional correlates are discussed.
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    URL: http://dx.doi.org/10.1007/BF00234868
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    PAS-positive cells of the pars intermedia are calcium-sensitive in the goldfish maintained in a hyposmotic milieu (1980)
    Springer
    Cell & tissue research 212 (1980), S. 29-38 
    Details
    ISSN: 1432-0878
    Keywords: Pituitary ; Pars intermedia ; Calcium ; Deionized water ; Teleosts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Cytological changes in the pars intermedia of the goldfish were investigated after adding calcium to deionized water (DW). In fish maintained in DW, the PAS-positive cells are highly stimulated in comparison to cells of fish kept in fresh water (FW). In DW supplemented with calcium at the same concentration as in FW (2 mM/l), the hyperactivity of the PAS-positive cells is prevented. When calcium ions are added 60 h before the animals are sacrificed, the PAS positive cells start to show signs of regression and their granules are stored: the release of the granular material appears to be suppressed by calcium. In the goldfish, the PAS-positive cells, homologous to a similar cell type in the eel, react only very weakly with the PAS technique. The name “calcium-sensitive cells” appears to be more appropriate in the goldfish for this particular cell type, secreting an unknown factor. This factor, different from the prolactin produced in the rostral pars distalis of the hypophysis, might be an equivalent of a “hypercalcin”.
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    URL: http://dx.doi.org/10.1007/BF00234030
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    Pinocytosis and locomotion of amoebae (1980)
    Springer
    Cell & tissue research 213 (1980), S. 9-20 
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    ISSN: 1432-0878
    Keywords: Pinocytosis ; Calcium ; Chlorotetracycline ; Fluorescence microscopy ; Amoeba proteus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The dynamics of Ca++ during induced pinocytosis were studied in Amoeba proteus using chlorotetracycline (CTC). The fluorescence of the Ca++ -CTC-complex was monitored by an image intensification system, which has certain advantages over standard equipment: (1) Living cells are not subjected to the damaging influence of intensive microscopic illumination, (2) fluorescent probes are not bleached during observation, and (3) the rapid dynamics of Ca++-fluxes can be recorded using short exposure times. The results demonstrate the existence of Ca++ bound to intracellular and extracellular sites of the cell membrane complex in normal locomoting and pinocytoting Amoeba proteus. The application of cations inducing pinocytosis causes a rapid decrease in the external CTC-fluorescence probably due to a release of Ca++ from the mucous layer. The degree of fluorescence intensity is correlated with the capacity of pinocytotic channel formation, i.e., the fluorescence decreases as the number of channels increases. During the phase of vesiculation a distinct fluorescence mainly restricted to the basal region of the channels is observed. Intracellular Ca++ was detected in close vicinity to the plasma membrane after both microinjection and external application of CTC. The internal CTC-fluorescence is slightly decreased during the induction phase of pinocytosis. The observations are in good agreement with previous results on the localization of Ca++-binding sites at the plasma membrane of Amoeba proteus and demonstrate the important role of Ca++-fluxes for the process of pinocytosis.
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    URL: http://dx.doi.org/10.1007/BF00236916
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    The ultrastructural development of the macrogamete ofEimeria stiedai (1980)
    Springer
    Protoplasma 104 (1980), S. 33-41 
    Details
    ISSN: 1615-6102
    Keywords: Development ; Eimeria stiedai ; Macrogamete ; Ultrastructural changes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The development of the macrogamete ofEimeria. stiedai in the epithelial cells of the bile ducts of rabbits was studied by electron microscopy. A macrogamete was first identified by the presence of a large central nucleus with prominent nucleolus, and subsequently by the appearance of wall forming bodies. The macrogamete was limited by an outer single membrane under which there were remnants of a second membrane. The parasitophorous vacuole, in which the macrogamete was located, was often narrow and it contained no intravacuolar-tubules or -folds. As macrogametogony proceeded wall forming bodies of Type I and II, canaliculi, electron pale spaces (lipid) and polysaccharide granules increased in number. Granular endoplasmic reticulum, mitochondria and Golgi bodies were present throughout.
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    URL: http://dx.doi.org/10.1007/BF01279366
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    Development of antennal sensilla during moulting inNeomysis integer (Leach) (Crustacea, Mysidacea) (1980)
    Springer
    Protoplasma 105 (1980), S. 53-67 
    Details
    ISSN: 1615-6102
    Keywords: Crustacean sensilla ; Development ; Sensilla structure ; Moulting
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The sensilla are associated with 6 enveloping cells. The innermost enveloping cell (e 1) secretes the dendritic sheath (=thecogen cell). All other enveloping cells are involved in the formation of the outer cuticular apparatus in secreting the cuticle of a definite region of the new hair shaft. The development of the new sensilla begins when an exuvial space expands between old cuticle and epithelium. The newly forming hair shafts lie folded back in an invagination of the epidermal tissue. Only a distal shaft part projects into the free exuvial space. The cuticle of the distal and middle shaft region is secreted by the three middle enveloping cells (e 2–e 4) (=trichogen cells), which are arranged around the dendritic sheath. The wall of the cylinder, in which the distal shaft is situated, is formed by the cuticle of the future proximal shaft region. It is secreted by the outer enveloping cells (e 5 and e 6). Furthermore, both enveloping cells form the hair socket (=trichogen-tormogen cells). The outer dendritic segments encased within a dendritic sheath run up through the newly formed hair shaft and continue to the old cuticular apparatus. The connection between sensory cells and old hair shaft is maintained until ecdysis. On ecdysis the old cuticle is shed and the newly formed shaft of the sensillum is everted like the invaginated finger of a glove. The dendritic sheath and the outer dendritic segments break off at the tip of the new hair shaft. Morphologically this moulting process ensures that the sensitivity of the receptors is maintained until ecdysis. The internal organization of the sensory cells shows no striking changes during the moulting cycle. An increased number of vesicles is accumulated distally within the inner dendritic segments and distributed throughout the outer segments of the dendrites. The cytoplasmic feature of the enveloping cells indicates that synthesis and release of substances for the cuticular apparatus of the new sensillum take place.
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    URL: http://dx.doi.org/10.1007/BF01279849
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    Description of understory development in a tree plantation with a new method of data structuring (1980)
    Springer
    Plant ecology 40 (1980), S. 185-191 
    Details
    ISSN: 1573-5052
    Keywords: Clustering ; Data-processing ; Michigan ; Numerical method ; Phytosociology ; Stratum ; Understory ; Development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary During May and June, 1974, relevés were obtained from 30 plantation stands in the Saginaw Forest in southeastern Michigan. The canopy trees in these plantations were planted between 1904 and 1938. The understory has developed naturally over the years. The forest plantations offer opportunities for study of the effects of the canopy on the structure and species composition of the understory. A new numerical method of data structuring was used, which is based on the detection patterns of associated species within the table of 30 relevés from Saginaw Forest plantations. The method employs two difference measures. DIF 1 uses presence/absence data and DIF 2 the cover/abundance values obtained from the relevés. Both difference measures supply distance values for every pair of relevés. The least dissimilar relevés are then grouped together by the farthest neighbor agglomerative algorithm of Lance & Williams (1967). The DIF 1 analysis was inconclusive, but the DIF 2 analysis gave coherent results. It has shown that relevés with similar canopies have similar understories. The differences in understories among the relevés are due less to species composition than to the relative abundances of the species.
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    URL: http://dx.doi.org/10.1007/BF00228485
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    Vegetation development in a former orchard under different treatments: A preliminary report (1980)
    Springer
    Plant ecology 43 (1980), S. 95-102 
    Details
    ISSN: 1573-5052
    Keywords: Aegopodion podagrariae ; Arrhenatherion elatioris ; Development ; Dynamics ; Experiment ; Fertilization ; Mowing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Vegetation development in a former orchard on loamy moist soil after removal of the fruit trees under different treatment is described with the help of multivariate methods. Data on 20 plots over 9 years are treated with a simple classification program and a PCA ordination. Two main groups of plots are involved: one in a relatively moist part of the area where the trees were removed in 1970, one in a slightly drier area where the trees had been removed earlier. The first group is characterized by forbs such as Urtica dioica and Lamium maculatum, the second by grasses, mainly Arrhenatherum elatius. Under a mowing regime the plots of the first group change in the direction of the second group. Under continued fertilization the Urtica vegetation can be maintained. Establishment and/or rapid spread of Urtica can be brought about in Arrhenatherum dominated sites by (re-) introduction of nitrogen fertiliser. Not mowing leads to a dense Urtica-Lamium stand in which Lamium maculatum finds an optimum some years after mowing is stopped. The methods of summarizing the site-time data are discussed and some new ones announced for further research.
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    URL: http://dx.doi.org/10.1007/BF00121022
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    Effects of acid irrigation and liming on two clones of Norway spruce (1980)
    Springer
    Plant and soil 57 (1980), S. 305-321 
    Details
    ISSN: 1573-5036
    Keywords: Aluminium ; Acid precipitation ; Calcium ; Calcium carbonate ; Clone ; Iron ; Magnesium ; Manganese ; Nitrogen ; Picea abies ; Phosphorus ; Potassium ; Sulphate ; Sulphur
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effect of acid irrigation on the growth of rooted cuttings ofPicea abies (L.) Karst, was investigated in a pot experiment lasting 3 years. It involved two clones of Norway spruce, H 253 Bogstad I and H 254 Bogstad II. Irrigation water of pH 5.4, 4.0, 3.0 and 2.5 was used. Liming was included in the experiment. After the experimental period, the plants of all treatments were growing reasonably well. However, those plants irrigated at pH 2.5 were slightly discoloured. The plant mortality was only 3% throughout the experiment, and was not connected to acid irrigation. The limiting growth factor was N. All other nutrient elements measured in the plants were close to optimal concentration. Plants irrigated at pH 2.5, and to some extent at pH 3, contained excessively high concentrations of Al, t-S and SO4. The total amount of Ca, Fe and Mn taken up by the plants decreased with increasing soil acidity. The increased growth of clone H 254 relative to H 253, produces a corresponding impression on soil characteristics. Soil acidity is governed by acid irrigation and CaCO3 application, but the clonal effects are also of importance. Norway spruce appears to be tolerant to Al concentrations as high as 50 mmol/kg in the needles.
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    URL: http://dx.doi.org/10.1007/BF02211689
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    Elemental concentrations in plant tissues as influenced by low pH soils (1980)
    Springer
    Plant and soil 55 (1980), S. 157-161 
    Details
    ISSN: 1573-5036
    Keywords: Acid mine drainage ; Aluminum ; Betula nigra ; Calcium ; Magnesium ; Manganese ; Plant tissue analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Soils influenced by acid mine drainage (pH〈5.0) are characterized by low concentrations of essential nutrients and increased solubility of heavy metals. The conditions typically reduce plant establishment and growth. However, river birch (Betula nigra L.) is commonly found along low pH streams in southeastern Ohio. The objective of this study was to determine the concentration of Al, Mn, Ca and Mg inB. nigra tissues. The results indicate Al and Mn are accumulating inB. nigra when compared to other species. Within river birch, Al concentrations are highest in roots; Mn concentrations are highest in leaves. There is not a concomitant reduction in Ca and Mg concentrations as suggested by soil levels.
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    URL: http://dx.doi.org/10.1007/BF02149720
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    pH optima for crop growth (1980)
    Springer
    Plant and soil 54 (1980), S. 339-357 
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    ISSN: 1573-5036
    Keywords: Calcium ; Cassava ; Chemical composition ; Control of solution pH ; Copper ; Flowing solution culture ; French bean ; Ginger ; Hydrogen ion injury ; Magnesium ; Maize ; Manganese ; Nitrogen ; Optimum pH range ; pH ; Plant growth ; Root weight ratio ; Tomato ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Ginger, cassava, maize, wheat, french bean and tomato were grown for periods up to six weeks in continuously flowing nutrient solutions at seven constant pH values ranging from 3.3 to 8.5. All species achieved maximum or near-maximum growth in the pH range 5.5 to 6.5. However, there were substantial differences in the ability of species to grow outside this range. Ginger and cassava were the most tolerant species to low solution pH, while ginger and tomato were the only species to show no yield depression at the highest solution pH. Roots of all species at pH 3.3 and some species at pH 4.0 exhibited symptoms of hydrogen ion injury. In addition, the concentrations of magnesium in the tops of all six species, of nitrogen in the tops of tomato and cassava, and of manganese in the tops of maize at these pH values were inadequate for optimal growth. Growth depression at high solution pH was associated with iron deficiency in maize and wheat and with nitrogen and/or copper deficiency in cassava. The relevance of the present results to crop growth under field conditions is discussed. The complex interplay of plant and soil characteristics militates against precise definition of an optimum pH range for the growth of a particular crop unless the soil is also specified.
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    Association of cation and organic anion accumulation with iron chlorosis of Scots pine on prairie soils (1980)
    Springer
    Plant and soil 56 (1980), S. 293-300 
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    ISSN: 1573-5036
    Keywords: Calcareous soils ; Calcium ; Cation-anion balance ; Induced-iron chlorosis ; Nitrate ; Organic anions ; Pinus sylvestris L ; Prairie
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Iron chlorosis of 4 year old Scots pine (Pinus sylvestris L.) in comparison to areas of adjacent healthy growth on calcareous prairie soil, was associated with slight increases in the soluble ion content of the saturation paste extract. Such increases in soluble ions (mainly calcium sulphate) were associated with significant increases in ash, cation (including iron) and organic anion content of the chlorotic needles. Increasing levels of available soil nitrate were also related to increase in organic anions. Nitrogen and phosphorus assimilation was adversely affected under conditions of iron chlorosis. These observations support the theory of induced iron deficiency associated with elevated levels of organic anions or translocated cations and are applicable to plantings of conifers on prairie soils.
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    URL: http://dx.doi.org/10.1007/BF02205858
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    Effects of artificial acid rain on decomposition of spruce needles and on mobilisation and leaching of elements (1980)
    Springer
    Plant and soil 56 (1980), S. 365-378 
    Details
    ISSN: 1573-5036
    Keywords: Acid rain ; Calcium ; Decomposition ; Leaching ; Magnesium ; Manganese ; Mobilisation ; Nitrogen ; Phosphorus ; Potassium ; Spruce litter
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Dry matter and chemical changes in decomposing spruce needles were investigated after 16 and 38 weeks in laboratory lysimeters treated with distilled water or distilled water acidified to pH 3 or 2 with sulphuric acid. The water was added twice weekly in quantities equal to 100 or 200 mm month−1. The CO2 evolution and leaching of P, K, Mg, Mn, and Ca was followed together with pH measurements of the leachate. The loss of dry matter was approximately 25% during the first 16 weeks and approximately 37% after 38 weeks. At the first samling, 16 weeks, the amount of material decomposed was greater from the lysimeters given 100 mm month−1 of water. At this water quantity dilute sulphuric acid increased the decomposition. After 38 weeks sulphuric acid at pH 3 and 2 had decreased the decomposition at 200 mm month−1. However, the effects of acid application were small. The effect of treatment using acidified water on the content of monosaccharides was not consistent, whereas there was an indication of reduced decomposition of lignin when treated with 200 mm water month−1 at pH 3 and 2. Nitrogen was conserved in the lysimeters with small differences between the various treatments. The order of mobility of metal elements was K〉Mg〉Mn〉Ca. Increasing the quantity of water increased the leaching of K especially, whereas addition of dilute sulphuric acid increased the leaching of Mg, Mn and particularly Ca. During the first 16 weeks of the experiment, sulphuric acid reduced the leaching of P while later on this treatment increased the leaching. The pH of the leachate from the lysimeters treated with distilled water was initially 4.0–4.6 increasing to approximately 6.6 after 22 weeks. The pH of the decomposed needle material was 4.6 and approximately 5.2 after 16 and 38 weeks respectively. When treated with water at pH 3 the pH of the leachate was between 4 and 5, and the pH of the needles 4.2–5.1. Treatment with water at pH 2 gave a leachate with pH just above 2 and decreased the pH of the needles that had received 200 mm ‘rain’ month−1 to 2.9. The effect of the artificial acid rain appears to be more pronounced on the leaching of metal elements than on the biological activity and the dynamics of N and P. The treatments must be considered extreme when compared with the acidity of natural rain.
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    Aluminium effects on growth and Al, Ca, Mg, K and P levels in triticale, wheat and rye (1980)
    Springer
    Plant and soil 57 (1980), S. 467-470 
    Details
    ISSN: 1573-5036
    Keywords: Aluminum concentration ; Aluminum toxicity ; Calcium ; Magnesium ; Nutrient solution ; Phosphorus ; Potassium ; Triticale ; Rye ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effects of A1 on the growth and mineral composition of different cultivars of triticale (X Triticosecale, Wittmack), wheat (Triticum aestivum L.) and rye (Secale cereale L.) growing in 1/5 strength Steinberg solutions containing 0 or 6 ppm A1 were evaluated after 32 days. Aluminum increased the concentrations of P and K in the roots and K in the tops of most of the cultivars tested. A1 tolerant triticale retained a lower concentration of Mg in the roots and tops than the A1 sensitive triticale, when subjected to A1 stress. In addition, A1 treatments resulted in smaller increases in root P for the A1 tolerant triticale than for the A1 sensitive cultivars. The concentration of root Ca and P of the A1 tolerant wheat cultivars were significantly below that of the more sensitive plants. Aluminum tolerance in rye appeared to be associated with lower Ca and higher Mg concentrations in the tops. The accumulation of P and A1 in the roots was characteristic of sensitivity in triticale, wheat and rye.
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    URL: http://dx.doi.org/10.1007/BF02211704
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    Effect of dietary cholecalciferol deficiency on plasma thyroid hormone concentrations in rainbow trout,Salmo gairdneri (Pisces, Salmonidae) (1980)
    Springer
    Environmental biology of fishes 5 (1980), S. 167-173 
    Details
    ISSN: 1573-5133
    Keywords: Vitamin D ; Thyroid gland ; Growth ; Endocrines ; Trout ; Calcium ; Thyroxine ; Triiodothyronine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Synopsis Diets deficient in vitamin D effected a significant increase in plasma triiodothyronine (T3) concentration in raibow trout (Salmo gairdneri); different levels of dietary calcium exerted no effect on plasma T3 levels. These effects of vitamin D deficiency on plasma T3 levels appeared to be reversible, vitamin D supplementation after a period of vitamin D deficiency lowered T3 levels. Vitamin D3, vitamin D2 and the metabolites 25(OH)-D3 and 1, 25(OH)2D3 were all effective in lowering plasma T3 levels; vitamin D3 appeared to be more effective than vitamin D2. There appeared to be a correlation between weight gain and plasma T3 concentration in the groups fed different types and levels of vitamin D supplementation suggesting that the increased T3 levels may be a compensatory increase to the reduced weight gain of the vitamin D deficient fish. Plasma T4 levels were not affected by dietary vitamin D deficiency.
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    URL: http://dx.doi.org/10.1007/BF02391624
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  • 36
    Electronic Resource
    Electronic Resource
    The susceptibility of terrestrial gastropods to experimental infection withElaphostrongylus rangiferi Mitskevich (Nematoda: Metastrongyloidea) (1980)
    Springer
    Parasitology research 62 (1980), S. 7-14 
    Details
    ISSN: 1432-1955
    Keywords: Elaphostrongylus rangiferi ; Rangifer tarandus ; Gastropods ; Susceptibility ; Development
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Sixteen species of terrestrial gastropods, collected at Tromsöya, northern Norway, were experimentally brought into contact with first stage larvae ofElaphostrongylus rangiferi, a parasite causing neurological disorders in reindeer,Rangifer tarandus (L.). All species were susceptible to infection but there were considerable variations in incidence and intensity of infections and in the development of the nematode in the different gastropod species. InDiscus ruderatus, Arion silvaticus, Deroceras laeve, Euconulus fulvus, andTrichia hispida, development to infective third stage larvae took about 20 days at 20°C. InSuccinea pfeifferi, Deroceras reticulatum andArianta arbustorum development was somewhat slower. InCochlicopa lubrica, Arion subfuscus, A. hortensis, Vitrina pellucida, Nesovitrea spp., andClausilia bidentata, only small numbers of larvae developed and at a very slow rate. InVertigo lilljeborgi andPunctum pygmaeum, the larvae did not develop. Growth in length ofE. rangiferi inD. ruderatus was rapid and continuous up to the second molt (about 15 days postinfection). Thereafter there was a slower growth rate. Tissue reactions against the infection were observed in eleven of the gastropod species. No obvious relationship between the degree of tissue reactions and susceptibility to infection was found.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00925362
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  • 37
    Electronic Resource
    Electronic Resource
    Multiple effects of Na+ removal on pancreatic secretion in vitro (1980)
    Springer
    Cell & tissue research 210 (1980), S. 295-303 
    Details
    ISSN: 1432-0878
    Keywords: Pancreas ; Stimulus-secretion coupling ; Sodium ; Calcium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The effects of removing Na+ from the incubation medium on basal and secretagogue induced zymogen release by pancreatic fragments and isolated pancreatic acini were studied by both morphological evaluation and measurement of amylase release. In both fragments and isolated acini, removal of Na+ led to an increased basal secretion of zymogen granule contents from acinar cells via exocytosis; secretory material, however, accumulated in acinar and ductular lumina as a result of the lack of fluid secretion necessary to wash out the enzymes. In studies with fragments, after Na+ removal there was no significant increase in amylase release into the medium; isolated acini, in contrast, showed an increased amylase release consistent with the shorter distance from the acinar lumen to the bathing medium. Stimulation with either bethanechol or caerulein led to a further depletion of zymogen granules in both preparations; in the absence of Na+ secretory product accumulated in intracellular lakes as well as in duct lumens. The hypothesis that Na+ influx is important in stimulus-secretion coupling to release intracellular Ca2+ was directly tested by measuring 45Ca2+ efflux. No effect of removing Na+ on 45Ca2+ efflux was seen. It was concluded, therefore, that while Na+ is essential for pancreatic fluid secretion, it is not necessary for the secretion of zymogen granule contents into acinar lumina.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00237617
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  • 38
    Electronic Resource
    Electronic Resource
    The programming of silk-gland development in Bombyx mori (1980)
    Springer
    Cell & tissue research 213 (1980), S. 311-324 
    Details
    ISSN: 1432-0878
    Keywords: Bombyx ; Silk-gland ; Starvation ; Development ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The cytological development of the silk gland has been studied by light and electron microscopy in silkworms experimentally starved at different periods of the natural feeding stage during the fifth instar. When newly molted animals are not provided with food, no sign of growth is observed. Starvation initiated early during the obligatory feeding period, stops cell growth and development of the organelles involved in protein synthesis and secretion, whereas it induces the appearance of organelles concerned with autolysis. These effects are reversible if starvation is not prolonged beyond two days. Starvation during the facultative feeding period, at the time of massive fibroin production, results in quantitative and qualitative modifications of organelles related to the decrease of fibroin production and the onset of autolysis. Rough endoplasmic reticulum, responsible for fibroin synthesis, forms transitory whorls. Fibroin transport via the Golgi apparatus and secretion of the protein into the gland lumen decrease parallel to fibroin synthesis, so that no fibroin storage can be detected in any organelle. After food deprivation, autophagosomes and secondary lysosomes rapidly develop in the cytoplasm, and if starvation continues portions of the cytoplasm are sequestered and completely destroyed. If animals are refed, fibroin production is resumed and autolysis declines. These ultrastructural alterations of the silk gland during experimental starvation are very similar to those observed during the periods of physiological starvation (molt and cocoon spinning) and generally considered to be under hormonal control. Our results raise the question of the nature of interactions between alimentary and hormonal factors which control silk-gland development.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234790
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