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  • Articles  (14)
  • Rhizobium  (14)
  • 1980-1984  (14)
  • 1945-1949
  • 1982  (10)
  • 1980  (4)
  • Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition  (14)
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  • Articles  (14)
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  • 1980-1984  (14)
  • 1945-1949
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 54 (1980), S. 95-106 
    ISSN: 1573-5036
    Keywords: Acid soil ; Antibiotic resistance ; Competition ; Rhizobium ; Soybean ; Symbiotic nitrogen fixation ; Tropical Africa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The efficacy of inoculating soybean cvs. TGm294-4 and TGm80 in an acid soil (pH 4.6, water) was studied in a field trial in Nigeria.Rhizobium japonicum strains CB1809str r and 46spc r which had been selected for symbiotic competence in the acid soil were used as inocula. Other treatments included no inoculation, combined nitrogen (150 kg N ha−1) and lime (1 t ha−1). Plants grown in acid soil without inoculation and lime had very few nodules, were nitrogen deficient and grain yields were poor (0.3–0.4 t ha−1). Yield responses to combined nitrogen were generally small due to fertilizer loss through leaching. Inoculation greatly increased nodulation, shoot dry matter and shoot N%; grain yields were between 1.6 and 2.0 t ha−1. Lime improved all harvest measurements in the uninoculated and nitrogen treatments but not in the inoculated treatments. In a duplicate trial without lime on near-neutral soil, similar inoculation responses were obtained at 6 weeks. However, at final harvest there were no significant differences in grain yield between treatments due to late, effective nodulation by unidentified strains in the uninoculated treatments. Assays for strain identity confirmed the successful establishment of the inocula in both soils, with 100% of nodules from inoculated plants containing an introduced strain.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 56 (1980), S. 33-37 
    ISSN: 1573-5036
    Keywords: Autochthonous ; Nodule bacteria ; Nodulation ; Plantations ; Rhizobium ; Shelter belts ; Tree legumes ; Wind breaker
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Twenty five species of tree legumes, belonging to genera Acacia, Albizzia, Bauhinia, Colophospermum, Dichrostachys, Leucaena, Peltophorum, Pithecolobium and Prosopis were examined for nodulation under pot culture conditions using unsterilized soil. Out of these, only nineteen tree-legumes nodulated. It was interesting to note that all the three species of Bauhinia and one each of Acacia, Colophospermum and Peltophorum did not nodulate both at Jodhpur and Delhi. Inoculation of tree legumes with their own rhizobial isolates, improved nodulation under sterilized conditions.
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 56 (1980), S. 491-494 
    ISSN: 1573-5036
    Keywords: Aeschynomene ; Azospirillum ; Rhizobium ; Stem nodules
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Fifteen isolates of nodule bacteria were isolated from root and stem nodules ofAeschynomene aspera and they were characterized as Rhizobium by well known laboratory tests. All these isolates together with other efficient strains of known rhizobia belonging to different cross-inoculation groups were evaluated for their nodulation abilities onAeschynomene aspera, Cajanus cajan (pigeon pea),Cicer arietinum (chickpea),Pisum sativum (pea),Trifolium repens (clover),Medicago sativa (lucerne),Lens culinaris (lentil),Glycine max (soybean),Vigna sinensis (cowpea),Vigna radiata (mung bean),Vigna mungo (urd bean) andArachis hypogea (peanut). The results demonstrated that Rhizobium fromAeschynomene could form nodules only on its homologous host (Aeschynomene) but not on other legumes tested. Secondly, none of the rhizobia of other cross-inoculation groups could nodulateA. aspera.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 67 (1982), S. 293-303 
    ISSN: 1573-5036
    Keywords: Burning ; Denitrification ; N-cycling ; N2-fixation ; Nitrification ; Oxisol ; Rhizobium ; Savanna ; South America ; Ultisol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Resumen Las sabanas ocupan alrededor de 300 millones de hectáreas de Sudamérica. Los suelos son básicamente oxisoles y ultisoles de muy baja fertilidad y alta acidez. La vegetación natural varía en densidad y en la cantidad de biomasa producida anualmente, la cual puede llegar a ser igual a la producida por bosques de la región. Entre los microorganismos fijadores de nitrógeno, los únicos bien estudiados son las bacterias del género Rhizobium. En el manejo de la biomasa de estas áreas, es importance considerar la fijación del nitrógeno, como una fuente posible que reemplace al que fué exportado en las cosechas. La nitrificación y la denitrificación en estos, es intensa pero no bien estudiada. La distribución de lluvias durante la estación de crecimiento parece tener una influencia considerable en la provisión de nitrógeno de los suelos. Se registran considerables pérdidas de nitrógeno en este ambiente, cuando amplias áreas son quemadas anualmente.
    Notes: Abstract Savannas cover about 300 million hectares of South America. The soils are mainly oxisols and ultisols and their natural fertility is very low with high acidity. The natural vegetation varies in density and in the amount of biomass produced annually, which can be equal to that produced by forests in the region. Among the nitrogen-fixing micro-organisms, the only ones well-studied are Rhizobium bacteria. In managing the biomass in these areas, it is important to consider biological nitrogen-fixation as a possible source of nitrogen to replace that removed in crops. Nitrification and denitrification in these soils are intense but not well studied. The rainfall distribution during the growing season seems to have a considerable influence of the nitrogen supply to the soils. A considerable loss of nitrogen occurs in this environment when vast areas are burned annually.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 55 (1980), S. 163-165 
    ISSN: 1573-5036
    Keywords: Acetylene reduction ; Nitrogen fixation ; Rhizobium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A method is described to demonstrate nitrogen fixation by free-living Rhizobium cells. After aerobic growth in a nutrient solution, the bacteria are centrifuged. Acetylene reduction by the rhizobial cells in the pellet can be measured within a few days. Hydrogen gas frequently stimulates acetylene reduction.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 65 (1982), S. 133-135 
    ISSN: 1573-5036
    Keywords: Green gram ; Nitrogenase-activity ; Pathogenesis ; Rhizobium ; Rhizoctonia ; Specific activity ; Symbiosis ; Vigna radiata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A pot experiment, using cowpea Rhizobium and one percent inoculum ofRhizoctonia botaticola (Taub.) Butler was carried out on green gram (Vigna radiata cv. PS 16) to study the interaction between symbiosis and root rot in terms of plant growth and nitrogen fixation. The microtomy of the infected roots showed distortion of the outer layers of root as a possible cause for antagonistic interaction of these two bio-processes.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 66 (1982), S. 413-416 
    ISSN: 1573-5036
    Keywords: Growth ; Nematicide ; Nitrogen fixation ; Nodulation ; Peanut ; Rhizobium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effect of two granular nematicidesviz. oxamyl and fenamiphos, on the nodulation and growth of Rhizobium inoculatedArachis hypogaea L. was studied in glasshouse and field trials. In the glasshouse trial at the suggested rates of application shoot fresh weight was significantly reduced by oxamyl whilst root fresh weight was similarly affected by fenamiphos. In the field trial vegetative growth and plant emergence were significantly reduced by both nematicides. Nodulation at the higher rates of application was increased by both oxamyl and fenamiphos whilst oxamyl caused a significant increase in pod number at the highest rate of application.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 66 (1982), S. 439-442 
    ISSN: 1573-5036
    Keywords: N2-fixation ; Nodulation ; Rhizobium ; Soybean ; Symbiosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The effect of mineral nitrogen on establishment and activity of symbioses between soybean and several strains ofRhizobium japonicum and on the establishment of nodules ofR. japonicum isolated from nodules of field crops is studied. All strains were highly susceptible to the effects of 200 ppm NO3−N on the establishment of symbiosis; 50 ppm NO3−N had little effect. Response of symbioses establishhed in the absence of mineral N to short term exposure to nitrate or ammonium varied significantly between strains. Nodule isolates from soybean crops growing in nitrifying soil were no less susceptible to the inhibitory effects of mineral N on nodule formation than a laboratory culture of the commercial inoculant strain.
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  • 9
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 67 (1982), S. 227-239 
    ISSN: 1573-5036
    Keywords: Extension ; Inoculant production ; Latin America ; N2-fixation ; Rhizobium ; Training
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Description / Table of Contents: Resumen Más de 60 instituciones y 100 investigadores están trabajando de la investigación con Rhizobium en América Latina. La mitad de los investigadores están localizados en Argentina y Brasil. La actividad de investigación y la aplicación de los conocimientos científicos varían ampliamente de acuerdo con los paises. Los problemas de la investigación incluyen: 1) Entrenamiento inadecuado de los investigadores y poca atención para la simbiosis Rhizobium/Leguminosas en las escuelas de Agronomía; 2) investigaciones de baja prioridad sin consideración para las necesidades más inmediatas para los agricultores tales como identificación de los factores limitantes ambientales (por ejemplo: deficiencias nutricionales), técnicas para la producción de inoculantes en pequeña escala y poco control de calidad de los inoculantes disponibles; 3) aislamiento de los investigadores e insuficiente apoyo de literatura; 4) baja interdisciplinaridad en las investigaciones (por ejemplo: en muchas instituciones los investigadores son microbiológos sin conocimientos de agronomía, o agrónomos sin entrenamiento en microbiología y 5) insuficiente diseminación de los conocimientos científicos. Los problemas de la producción y control de inoculantes incluyen: 1) las cepas empleadas en los inoculantes (nacionales o importadas) no son seleccionadas localmente; 2) poco control de calidad de los inoculantes y como resultado, inoculantes malos traen descrédito para la práctica de la inoculación, y 3) precios muy altos de los inoculantes. Los problemas de la extensión incluyen: 1) falta o deficiencia de los programas de promoción de leguminosas por las organizaciones gubernamentales, 2) poco contacto entre los investigadores y los extensionistas y 3) administradores líderes, extensionistas y agrónomos que trabajan en el campo no poseen adecuados conocimientos sobre la simbiosis Rhizobium/Leguminosas. Algunas medidas inmediatas para promover la extensión y programas de promoción de las leguminosas y/o control oficial de la calidad de los inoculates son necesarias en Argentina, Uruguay, Brasil, México y posiblemente Colombia. Perú, Venezuela, Costa Rica y Chile necesitan esfuerzos combinados dirigidos prioritariamente para promover la investigación y extensión. En Ecuador, Paraguay, Bolivia, Nicaragua, Honduras, Guatemala, República Dominicana y Panamá, la prioridad debe ser dada para la investigación. Colombia debe ser incluída en este grupo por la razón de que las instituciones nacionales deben ser fortalecidas. La tabla 2 relaciona estas prioridades con mas detalles.
    Notes: Abstract More than 60 institutions and 100 researchers were involved in Rhizobium research in 1978 in Latin America. Half of these researchers were located in Argentina and Brazil. Research activity and the application of research findings vary widely among countries. Problems that plague research include 1) inadequate training of research personnel and insufficient attention paid to the Rhizobium/Legume symbiosis at agriculture schools; 2) poorly-established research priorities that do not sufficiently weigh the immediate needs for the farmers such as the identification of limiting environmental factors (e.g. nutritional deficiencies), techniques for smallscale inoculant production, and quality control of available inoculants; 3) isolation of the researchers and a lack of adequate library support; 4) poorly integrated research teams (e.g. in many institutes researchers are either microbiologists with no agricultural background or agronomists lacking microbiological training); and 5) insufficient dissemination of research findings. Problems with inoculant production and control include 1) a local dependence on national or imported inoculants rather than on locally-selected strains, 2) poor inoculant quality control which results in low inoculation success rates and subsequent discredit to the inoculation practice, and 3) high prices for inoculants. Extension problems include 1) lacking or deficient legume-promotion programs by government agencies, 2) poor contact between research and extension workers, and 3) administrators, leaders, extension workers and agronomists working in the field that lack adequate knowledge of the Rhizobium/Legume symbiosis. Immediate measures to foster extension and legume promotion programs and informal and/or official quality control are needed in Argentina, Uruguay, Brazil, Mexico, and probably Colombia. Countries where combined efforts should primarily be directed toward stimulating research and extension include Peru, Venezuela, Costa Rica, and Chile. In Ecuador, Paraguay, Bolivia, Nicaragua, Honduras, Guatemala, the Dominican Republic and Panama, priority should be given to research. Colombia should also be included in this group as national research institutions need to be strengthened. Table 2 lists these priorities more fully.
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  • 10
    ISSN: 1573-5036
    Keywords: Glycine max ; Iron deficiency ; Mycorrhizae ; Nodulation ; Platte ; Valley ; Yellows ; Rhizobium ; Soybeans
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A chlorotic condition of soybeans in the river valleys of eastern Nebraska was studied. The pattern of chlorosis, presence of petiolar necrosis, reductions in rhizobial and mycorrhizal infection, and plant and soil chemical analyses suggest that the condition is more complex than normal iron deficiency chlorosis or other mineral deficiencies or toxicities. Nodulation is almost totally inhibited in affected plants. Mycorrhizal fungi, while able to infect early in the season, do not persist on affected plants. Breakdown of endodermis was observed in the roots of affected plants. The factors contributing to this condition have not yet been determined.
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  • 11
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 69 (1982), S. 163-169 
    ISSN: 1573-5036
    Keywords: Biodegradation ; Catechin ; p-Hydroxybenzoate ; Oxidation ; Rhizobium ; Salicylate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The ability of rhizobia to utilize catechol, protocatechuic acid, salicylic acid, p-hydroxybenzoic acid and catechin was investigated. The degradation pathway of p-hydroxybenzoate byRhizobium japonicum, R. phaseoli, R. leguminosarum, R. trifolii andRhizobium sp. isolated from bean was also studied.R. leguminosarum, R. phaseoli andR. trifolii metabolized p-hydroxybenzoate to protocatechuate which was cleaved by protocatechuate 3,4-dioxygenasevia ortho pathway.R. japonicum degraded p-hydroxybenzoate to catechol which was cleaved by catechol 1,2-dioxygenase.Rhizobium sp., a bean isolate, dissimilatedp-hydroxybenzoate to salicylate. Salicylate was converted to gentisic acid prior to ring cleavage. The rhizobia convertedp-hydroxybenzoate to Rothera positive substance. Catechol and protocatechuic acid were directly cleaved by the species.R. japonicum converted catechin to protocatechuic acid.
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  • 12
    ISSN: 1573-5036
    Keywords: Isotope techniques ; Nitrogen-15 ; Nitrogen fixation ; Nitrogen utilization ; Phaseolus vulgaris ; Rhizobium ; Varietal comparison
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Differences in N2-fixation byPhaseolus vulgaris bean cultivars were successfully evaluated in the field using15N isotope dilution technique with a non-fixing test crop of a different species (wheat). The Phaseolus cultivars could have been similarly ranked for N2-fixation capacity from either seed yield or total nitrogen yield, but the isotope method provided a direct measure of N2-fixation and made it possible to estimate the proportion of fixed to total nitrogen in the crop and in plant parts. Amounts of nitrogen fixed varied between 24.59 kg N/ha for the 60-day cultivar Goiano precoce to 64.91 kg N/ha for the 90-day cultivar Carioca. The per cent of plant nitrogen due to fixation was 57–68% for the 90-day cultivars and 37% for Goiano precoce (60-day cultivar). Fertilizer utilization was 17–30% of a 20 kg N/ha fertilizer application. 100 kg N/ha fertilizer application decreased N2-fixation without suppressing it totally. Differences in yield between the highest yielding (Carioca) and the lowest (Moruna) 90-day cultivars were also due apparently to varietal differences in efficiency of conversion of nitrogen to economic matteri.e. seed, as well as to differences in capacity of genotypes for N2-fixation.
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  • 13
    Electronic Resource
    Electronic Resource
    Springer
    Plant and soil 65 (1982), S. 425-428 
    ISSN: 1573-5036
    Keywords: Chlorosis ; Cowpea ; Nodulation ; Rhizobitoxine ; Rhizobium
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary While screening cowpea rhizobia from West Africa for ability to nodulate various host species, foliar chlorosis was observed in young mung bean and soybean plants inoculated with certain strains. The chlorosis occurred in the first and sometimes the second trifoliate, but not on subsequent leaves. There was no correlation of symptoms with the presence of nodules. Where extreme chlorosis was induced in soybeans, there was stunting of the primary root. Disease symptoms were obtained with culture-broth supernatants free of rhizobia, indicating an extracellular toxin. In common with rhizobitoxine-producing strains ofR. japonicum, chlorosis-inducing cowpea strains were able to nodulate ‘non-nodulating’ soybeans of the rj1rj1 genotype.
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  • 14
    ISSN: 1573-5036
    Keywords: Breeding ; Cowpea ; Nitrogen nutrition ; Rhizobium ; Seed yield ; Symbiotic interactions ; Vigna unguiculata L
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Plant of cowpea (Vigna unguiculata (L.) Walp.) cv. TVu 1469 were grown in a plastic house set to simulate tropical temperatures. They were inoculated with one of two strains of Rhizobium and irrigated each day with nutrient solution either devoid of inorganic nitrogen (N) or containing 2.14 mM (30 ppm) N. Strain of Rhizobium significantly affected rates of dry matter and N accumulation as well as the total N content of mature plants. Variations in seed yield were due largely to Rhizobium effects on peduncle production and pod set on each peduncle, wheres inorganic N did not change these yield-determining components significantly. The agronomic and physiological implications of these data are discussed.
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