Publication Date:
1999-10-03
Description:
Precursors of alpha-defensin peptides require activation for bactericidal activity. In mouse small intestine, matrilysin colocalized with alpha-defensins (cryptdins) in Paneth cell granules, and in vitro it cleaved the pro segment from cryptdin precursors. Matrilysin-deficient (MAT-/-) mice lacked mature cryptdins and accumulated precursor molecules. Intestinal peptide preparations from MAT-/- mice had decreased antimicrobial activity. Orally administered bacteria survived in greater numbers and were more virulent in MAT-/- mice than in MAT+/+ mice. Thus, matrilysin functions in intestinal mucosal defense by regulating the activity of defensins, which may be a common role for this metalloproteinase in its numerous epithelial sites of expression.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Wilson, C L -- Ouellette, A J -- Satchell, D P -- Ayabe, T -- Lopez-Boado, Y S -- Stratman, J L -- Hultgren, S J -- Matrisian, L M -- Parks, W C -- New York, N.Y. -- Science. 1999 Oct 1;286(5437):113-7.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Pediatrics, Division of Allergy and Pulmonary Medicine, Washington University School of Medicine, St. Louis, MO 63110, USA. wilson_c@kids.wustl.edu〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/10506557" target="_blank"〉PubMed〈/a〉
Keywords:
Amino Acid Sequence
;
Animals
;
Catalysis
;
Cytoplasmic Granules/enzymology
;
Escherichia coli/growth & development
;
Escherichia coli Infections/immunology/microbiology
;
Female
;
Humans
;
*Immunity, Innate
;
*Immunity, Mucosal
;
Intestinal Mucosa/enzymology/immunology/microbiology
;
Intestine, Small/enzymology/*immunology/microbiology
;
Male
;
Matrix Metalloproteinase 7
;
Metalloendopeptidases/genetics/*metabolism
;
Mice
;
Molecular Sequence Data
;
Paneth Cells/enzymology
;
Protein Precursors/genetics/*metabolism
;
Recombinant Fusion Proteins/metabolism
;
Salmonella typhimurium/growth & development/pathogenicity
;
Tissue Extracts/pharmacology
Print ISSN:
0036-8075
Electronic ISSN:
1095-9203
Topics:
Biology
,
Chemistry and Pharmacology
,
Computer Science
,
Medicine
,
Natural Sciences in General
,
Physics
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