Publikationsdatum:
1998-06-20
Beschreibung:
Crystal structures of bovine heart cytochrome c oxidase in the fully oxidized, fully reduced, azide-bound, and carbon monoxide-bound states were determined at 2.30, 2.35, 2.9, and 2.8 angstrom resolution, respectively. An aspartate residue apart from the O2 reduction site exchanges its effective accessibility to the matrix aqueous phase for one to the cytosolic phase concomitantly with a significant decrease in the pK of its carboxyl group, on reduction of the metal sites. The movement indicates the aspartate as the proton pumping site. A tyrosine acidified by a covalently linked imidazole nitrogen is a possible proton donor for the O2 reduction by the enzyme.〈br /〉〈span class="detail_caption"〉Notes: 〈/span〉Yoshikawa, S -- Shinzawa-Itoh, K -- Nakashima, R -- Yaono, R -- Yamashita, E -- Inoue, N -- Yao, M -- Fei, M J -- Libeu, C P -- Mizushima, T -- Yamaguchi, H -- Tomizaki, T -- Tsukihara, T -- New York, N.Y. -- Science. 1998 Jun 12;280(5370):1723-9.〈br /〉〈span class="detail_caption"〉Author address: 〈/span〉Department of Life Science, Himeji Institute of Technology and CREST, Japan Science and Technology Corporation (JST), Kamigohri Akoh, Hyogo 678-1297, Japan.〈br /〉〈span class="detail_caption"〉Record origin:〈/span〉 〈a href="http://www.ncbi.nlm.nih.gov/pubmed/9624044" target="_blank"〉PubMed〈/a〉
Schlagwort(e):
Animals
;
Aspartic Acid/chemistry/metabolism
;
Azides/metabolism
;
Binding Sites
;
Carbon Monoxide/metabolism
;
Cattle
;
Copper/chemistry/metabolism
;
Crystallography, X-Ray
;
Electron Transport Complex IV/*chemistry/*metabolism
;
Heme/analogs & derivatives/chemistry/metabolism
;
Hydrogen Bonding
;
Hydrogen Peroxide/chemistry/metabolism
;
Hydrogen-Ion Concentration
;
Ligands
;
Metals/metabolism
;
Models, Chemical
;
Models, Molecular
;
Myocardium/*enzymology
;
Oxidation-Reduction
;
Oxygen/metabolism
;
Protein Conformation
;
*Proton Pumps
;
Tyrosine/chemistry/metabolism
Print ISSN:
0036-8075
Digitale ISSN:
1095-9203
Thema:
Biologie
,
Chemie und Pharmazie
,
Informatik
,
Medizin
,
Allgemeine Naturwissenschaft
,
Physik
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