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  • Life and Medical Sciences  (610)
  • ASTRONOMY  (384)
  • 1980-1984  (959)
  • 1925-1929  (35)
  • 1981  (959)
  • 1928  (35)
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  • 1980-1984  (959)
  • 1925-1929  (35)
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  • 101
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Vascular architecture and the structure of the intestinal hematopoietic centers of two cyclostomes, the hagfish Eptatretus burgeri and the ammocoetes larva of Entosphenus reissneri, are compared. Blood cells of the hagfish are generated in hematopoietic nests that develop around intestinal veins established primarily for transport of absorbed nutrients. In ammocoetes, on the other hand, blood cells are generated in hematopoietic nests of the typhlosole, closely associated with venous sinusoids developing around the longitudinally oriented mesenteric artery of the typhlosole. A collateral vein of the mesenteric artery is completed in the typhlosole after metamorphosis. Since the spleen of higher vertebrates develops in relation to establishment of the collateral vein of the largest foregut artery, the intestinal hematopoietic nests of ammocoetes may be regarded as a model of the primitive form of the spleen of higher vertebrates. Hematopoiesis in the hagfish intestine is not related to establishment of a collateral vein; hence „primitive spleen“ or „intestinal spleen“ may be improper terms in reference to the intestinal hematopoietic tissue of the hagfish.Morphological characteristics of the hematopoietic nests of the two cyclostomes are essentially the same. Blood cells of these nests are generated in the intervenous tissue, supported by interstitial connective tissue cells and reticulin fibers. Granulated cells are the most common type in the primitive hematopoietic nests. No definitive erythrothrombocytopoiesis has been identified. Lymphocytes have not been observed in the hagfish; however, small lymphocytes have been observed in the vascular lumen of sinusoids around the hematopoietic nests of ammocoetes. These lymphocytes probably originate outside of the typhlosole.
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  • 102
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    Journal of Morphology 170 (1981), S. 207-214 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: In this paper we describe the gross and microscopic anatomy of the internal carotid artery and demonstrate that this vessel does not directly supply blood to the brain, in the Monodontidae (order Cetacea). Our account is based on gross dissections and perfusion casts of the arterial vasculature in Delphinapterus leucas and Monodon monoceros and on histological material from the latter species.The internal carotid artery originates low in the neck and extends to the carotid rete at the base of the brain. The vessel tapers dramatically along its cervical course and changes from an artery elastic in nature to one more muscular. A single large cervical branch occurs in D. leucas and supplies cerebrally related retia in this region and prevertebral muscles. No cervical branches occur in M. monoceros. In otic regions, the internal carotid artery is small and muscular. A lumen is present; however, a split internal and external elastic lamella and a thickened subendothelial layer are evident. Though patent in the neck and ear, the vessel appears occluded within the carotid canal. At this level, the vessel is characterized by absence of a lumen and by fragmented elastic lamellae.We conclude that the internal carotid artery is anatomically closed at a level just proximal to the carotid rete and hence has no direct involvement with cerebral blood supply in the Monodontidae. Our results confirm other investigators' work on smaller cetacean species.
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  • 103
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    Journal of Morphology 170 (1981), S. 321-345 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Fiber type composition distinguished by the level of succinic dehydrogenase, mean fiber diameter, and fiber number were determined for the gracilis major and sartorius in ontogenetic series of the anurans Rana pipiens, Bufo americanus, and Xenopus laevis to characterize muscle composition and mode of muscle growth.A correlation can be made between the respective locomotor mode of these anurans, their activity physiology, and the fiber type composition of the propulsive muscles. R. pipiens and X. laevis have hindlimb muscles composed primarily of fast-twitch, fast-fatiguing type 1 fibers. B. americanus's muscles are composed of slow-twitch, slow-fatiguing type 2 and type 3 fibers. Fiber typing is apparently useful in explaining interspecific anuran locomotor capability previously difficult to correlate with body shape. Fiber typing also affords an explantion for reported variations in metabolism during strenuous activity.Postmetamorphically, anuran muscles grow by a combination of hyperplasia and hypertrophy, but the percent fiber type composition remains unchanged. In R. pipiens and X. laevis, the muscles grow primarily by hypertrophy with the addition of relatively fewer fibers. The converse is true for B. americanus. In all three species the combinations result in strong positive allometric increases in muscle cross section. Anuran muscle growth is similar to that reported for fish and different from that associated with mammals.
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  • 104
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The density of intramembranous protein particles was studied by freeze-fracture. Particle density on the fracture faces of the plasmalemma and the rough endoplasmic reticulum (RER), as well as the outer and inner membranes of the nucleus and the mitochondria in rat hepatocytes were quantified. Comparison among different age groups sampled days postcoitum (dpc), days postpartum (dpp), and months postpartum (mpp) shows age-related changes in particle density in each membrane system. With the exception of the RER, particle densities increased after the 16th dpc, reached a maximum at birth, and then decreased with increasing age. Simultaneously, the number nuclear pores shows a positive correlation with the particle density of the nuclear membranes. The particle density on the membranes of the RER shows a maximum on the 16th dpc, and on the 6th dpp. Thereafter, the density of the RER decreases slightly. In all membrane systems, the density of the particles on the external fracture faces is more variable than the density of the particles on the protoplasmic fracture faces.
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  • 105
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The muscular system in the posterior sucker of Branchiobdella pentodonta Whit. has circular, longitudinal and radial fibers. In the anterior sucker, which has circular and longitudinal fibers, the muscle system is scarce. Concentric fibers are found around the mouth. In both suckers the glandular elements form voluminous complexes secreting mucus for attachment to the substrate. Suckers show neuromuscular junctions and three distinct types of neuroglandular junctions: one with typical neurosecretory granules, one with larger neurosecretory granules produced by cells located at the origin of the segmental nerves, and one with presynaptic vesicles. The second type is peculiar to the posterior sucker. A comparison is made between suckers of Branchiobdella and those of leeches.
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  • 106
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    Journal of Morphology 170 (1981), S. 273-282 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Arboreal salamanders of the Neotropical genus Bolitoglossa are characterized by palmate, fully webbed feet. The feet act as adhesive structures enabling the salamanders to cling to smooth surfaces. Scanning electron microscopy of skin epithelium and light microscopy of serial sections show exceptionally smooth cell surfaces and increased numbers of mucous glands on the soles of the feet. These features enhance the abilities of the feet to adhere by means of surface tension and suction. They are part of a set of morphological characteristics that may have been produced as a result of paedomorphosis.
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  • 107
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The eyestalk of Astacus leptodactylus is investigated immunocytochemically by light, fluorescence, and electron microscopy, using an antiserum raised against purified crustacean hyperglycemic hormone (CHH). CHH can be visualized in a group of neurosecretory perikarya on the medualla terminalis (medulla terminalis ganglionic X-organ: MTGX), in fibers forming part of the MTGX-sinus gland tractus, and in a considerable part of the axon terminals composing the sinus gland. Immunocytochemical combined with ultrastructural investigations led to the identification of the CHH-producing cells and the CHH-containing neurosecretory granule type.
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  • 108
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    Journal of Morphology 169 (1981), S. 21-28 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Standard histological and SEM techniques have been used to examine the pair of statocyst organs located in the telson of the isopod, Cyathara polita. Each organ is formed as an invagination of the dorsal cuticle of the telson. The invagination narrows to form a stalk between the statocyst and dorsal surface. A canal courses longitudinally through this stalk and forms a continuous channel between the lumen of the cyst and the external environment. On the luminal floor of each statocyst, there are three pits; each correlates with a nodule protruding from the ventro-medial wall. From each pit, a single, bifurcating hair projects dorsally to contact the single concretion within the statocyst lumen. No other static organs have been found in this animal. Thus, maintenance of equilibrium in this species appears to be under the control of but six hairs, three in each statocyst. Innervation of each statocyst is provided by a branch of a nerve which connects anteriorly with the last abdominal ganglion.
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  • 109
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    Journal of Morphology 170 (1981), S. 215-237 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Hermaphroditism and sex reversal have not previously been ruled out adequately as possible explanations for the existence of unisexual species of reptiles. Therefore, we examined serial histological sections of the complete urogenital systems of nine F2 specimens belonging to two ontogenetic series of Cnemidophorus exsanguis raised in captivity in isolation from males, as well as the urogenital system of the F1 mother of one of these series. No evidence of spermatozoa or testicular tissue was found in any specimen. Comparative material reveals that the histology of the urogenital tract, including the mesonephric kidney in adults, is similar to that of females of bisexual species of Cnemidophorus. We conclude that C. exsanguis is a unisexual, parthenogenetic species whose normal reproduction does not involve sex reversal, self-fertilization, gynogenesis, hybridogenesis, or spermatozoa in any way whatsoever.
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  • 110
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    Cell Motility and the Cytoskeleton 1 (1981) 
    ISSN: 0886-1544
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
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  • 111
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    Cell Motility and the Cytoskeleton 1 (1981), S. 167-178 
    ISSN: 0886-1544
    Keywords: nerve growth ; actin ; tubulin ; antibodies ; immunofluorescence ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Embryonic chick nerve cells, from dissociated dorsal root ganglia, were cultured on polylysine substrata and examined for tubulin and actin distribution by indirect immunofluorescence.Antibodies generated against chick brain tubulin produced specific fluorescence in growth cones, neurites, and cell bodies without revealing distribution differences or substructure in the nerve cells. However, at reduced antitubulin concentrations, differences were resolved. Tubulin fluorescence remained uniform and intense in neurites and cell bodies, but exhibited reduced intensity and patterning in growth cones. Nonneuronal cells in the reduced intensity and patterning in growth cones. Nonneuronal cells in the cultures served as controls for typical cytoplasmic tubulin fluorescence distribution. Straining controls demonstrated that fluorescence resulted from tubulin-antitubulin binding.Analogous studies, using antibodies generated against chick brain actin, demonstrated distribution differences at reduced antiactin concentrations, including “hot spots” of intense fluorescence in growth cones and a paucity of fluorescence in neurites.
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  • 112
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    Cell Motility and the Cytoskeleton 1 (1981), S. 349-362 
    ISSN: 0886-1544
    Keywords: Ca2+ ; flagella ; symmetry ; vanadate ; spermatozoa ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Increased Ca2+ concentration causes a reversible increase in asymmetry of the flagellar bending waves of “potentially symmetric” demembranated sea urchin spermatozoa. When these flagella are immobilized with 5 μM vanadate, increased Ca2+ concentration causes a reversible increase in the total bend angle between the tip and the base of the immobilized flagella. These effects of Ca2+, and the movement which can be activated by CaATP2-, can be inhibited by vanadate, but in both cases, high concentrations of vanadate, of the order of 100 μM, are required. These observations suggest that ATP, possibly in the form of CaATP2-, is required for the Ca2+-induced change in shape of the flagella, but other observations suggest that the magnitudes of asymmetry and total bend angle are more closely related to Ca2+ concentration than to CaATP2- concentration.
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  • 113
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    Cell Motility and the Cytoskeleton 1 (1981), S. 363-370 
    ISSN: 0886-1544
    Keywords: Ca2+ ; Mg2+ ; symmetry ; flagella ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Potentially asymmetric spermatozoa are obtained when spermatozoa are demembranated in the presence of a low Ca2+/Mg2+ ion concentration ratio. They swim with asymmetric bending waves even when reactivated at low Ca2+ concentrations, and become more asymmetric when Ca2+ is increased. Potentially symmetric spermatozoa, which swim with symmetric bending waves at low Ca2+ and become asymmetric as the Ca2+ is increased, can be obtained by exposing the flagella to a high Ca2+/Mg2+ ratio, either during or subsequent to demembranation. The rate of this conversion is an increasing function of temperature and Triton concentration. Potentially symmetric spermatozoa can be reconverted to potential asymmetry, if the exposure to high Ca2+/Mg2+ is brief, and is terminated by addition of EGTA and Mg2+ before diluting the spermatozoa. The conversion to potential symmetry may involve removal of a labile component from the axoneme.
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  • 114
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    Cell Motility and the Cytoskeleton 1 (1981) 
    ISSN: 0886-1544
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
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  • 115
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    Cell Motility and the Cytoskeleton 1 (1981), S. 455-468 
    ISSN: 0886-1544
    Keywords: intercellular bridge ; intercellular communication ; cytokinesis ; squid ; ultrastructure ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Incomplete cytokinesis followed by the disappearance of the midbody and spindle remnant results in intercellular bridges between the cells of the blastoderm of the squid embryo. An electron microscope study of the morphology of the stages of development of the intercellular bridge is presented. Cytokinesis ceased as the furrow base reached a diameter slightly larger than the midbody. As furrowing stopped, a dense material accumulated to form a cylindrical sheath 50 nm thick, lining the inner surface of the furrow base. Proteolytic enzymes showed this material to have a significant protein component. As the midbody broke down, vesicles lined the inner surface of the bridge sheath. In this configuration, there was cyto-plasmic continuity between the cells, and organelles appeared to pass through the bridge.The intercellular bridge could become temporarily closed. Vesicles entered the channel and fused with the vesicles lining the inner surface of the sheath. The vesicles enlarged until the channel became occluded with a series of transverse cisternae, the edges of which were embedded in the material of the sheath. When the bridge reopened, the transverse cisterna appeared to dissociate from the sheath, move out of the channel, and break down. Occasionally bridges were seen in which the bridge wall appeared distorted into lobes. It is suggested that such bridges might be in the porcess of breaking down, resulting in the final separation of the cells.
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  • 116
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    Cell Motility and the Cytoskeleton 1 (1981), S. 499-515 
    ISSN: 0886-1544
    Keywords: dynein ; tubulin ; axonemes ; microtubules ; microtubule-associated proteins ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Microtubule-associated proteins (MAPs), isolated from brain tubulin, bound to and saturated outer fibers of Chlamydomonas flagella. MAPs present on these microtubules prevented the subsequent recombination of dynein. MAPs also bound to intact axonemes and thus did not specifically bind to the dynein binding sites on the A subfiber. A molar ratio of 1 mole MAP2 per 27 moles tubulin dimers at saturation of the outer fibers with MAP2 suggested that MAPs could effectively interfere with dynein recombination only if the MAPs were near the dynein binding sites to sterically prevent binding. However, electron microscopic observations indicated that MAPs were not localized but, instead, were dispersed around the outer fibers. In addition, MAP2 present at saturating amounts on in vitro assembled brain microtubules had no significant effect on dynein binding. Dynein-decorated microtubules contained clusters of arms suggesting that there may be cooperative interaction between the arms during dynein binding. Because the A subfiber of axonemes contains sites to which dynein preferentially attaches, MAPs may prevent recombination by interfering with cooperative binding to these specific sites. Dynein presumably binds with equal affinity to any protofilament on in vitro assembled microtubules, and, therefore, the MAPs may not be capable of effectively interfering with cooperative binding of dynein to these microtubules.
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  • 117
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    Cell Motility and the Cytoskeleton 1 (1981), S. 485-497 
    ISSN: 0886-1544
    Keywords: actin ; tubulin ; nucleotides ; polymerization ; microfilaments ; microtubules ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Both actin and tubulin, the major proteins of the cytoskeleton, bind nucleotide triphosphate (NTP) and exhibit the phenomenon of “polymerization-coupled” NTP hydrolysis. In this report I review the nature of polymerization-coupled NTP hydrolysis, and its possible role in the cellular function of actin and tubulin. Polymerization-coupled hydrolysis may be viewed as simply reflecting differences in the NTPase activity of free subunit as compared to polymer. Making assumptions concerning the values of various rate constants, it is possible to write expressions for the effects of NTP hydrolysis on the kinetics of polymerization. The role of NTP hydrolysis may be viewed in at least three different ways: (1) Hydrolysis alters the kinetics of assembly and disassembly. This leads to a consideration of the role of subunit flow in microtubule and microfilament function. (2) Hydrolysis is an essentially irreversible step that separates the assembly and disassembly reactions. This suggests a role of NTP in the regulation of polymer content during cellular cycles of assembly and disassembly. (3) NTP may allow transient stabilization of intersubunit bonds. This suggests a role of NTP in nucleation and possible regulation of nonequilibrium states of assembly.
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  • 118
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    Cell Motility and the Cytoskeleton 1 (1981), S. 193-203 
    ISSN: 0886-1544
    Keywords: polygonal network ; rat aortic smooth muscle cell ; cell culture ; electron microscopy ; amino acid analysis ; elastin ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Cultured rat aortic smooth muscle cells (SMC) were examined by electron microscopy and found to contain polygonal networks of 75 A° thin myofilament bundles. The cells also had large bundles of longitudinally aligned thin myofilaments with periodically spaced dense bodies. Abundant plasmalemmal vesicles were present at the cell periphery, and the cells were connected by desmosomes. Intercellular spaces contained sparse amounts of elastic fibers, a material generally present in SMC cultures. Analyses of amino acids by automated column-chromatography showed that isodesmosine and desmosine, two amino acid residues unique for elastin, were present. Accordingly, it was concluded that polygonal networks, previously detected solely in cultured nonmuscle cells, were present in SMC.Other findings suggest (1) a change in myofilament arrangement takes place during cell migration, and (2) rat aortic SMC grown in tissue culture flasks is an important experimental tool in the study of cell motility since such myofilament rearrangements were observed to occur up to fourteen days in first passage.
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  • 119
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    Cell Motility and the Cytoskeleton 1 (1981), S. 179-192 
    ISSN: 0886-1544
    Keywords: actin ; echinoderm ; fascin ; filopodia ; actin cross-linking protein ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Echinoderm coelomocytes transform from petaloid cells with large motile lamellipodia to filopodial forms. During this morphological transformation, actin filaments extensively reorganize from a random meshwork into tight bundles, which become the skeletons or cores of the filopodia. Antibody localization procedures show that fascin, a 58,000 dalton actin cross-linking protein, becomes incorporated into the filament bundles as they form. Isolated filopodial cores have a pronounced transverse striping pattern, which has been previously identified with fascin crosslinks, and gel electrophoresis identifies a protein in the cores that co-migrates with purified egg fascin. A few of the core fragments also have a distinctive “cap,” which we presume is the membrane insertion site for actin filaments.We have developed a radioimmunoassay for fascin and have used it to study the redistribution of this protein during transformation. Data from the assay indicate that fascin constitutes about 5% of the total cell protein and that substantially more fascin, approximately 1.5-2 times more, is found in the Triton-insoluble cytoskeletons of the filopodial cells than in the petaloid cells. Actin, measured by the DNAase I inhibition assay accounts for approximately 10% of the total cell protein. Approximately 65% of this actin is in a soluble non-filamentous form in the petaloid cells. Our results show that actin polymerization must occur during the cell shape change, since we find approximately 25% more actin in the filopodial cytoskeleton than in the petaloid cytoskeleton. The results show a preferential incorporation of fascin into the cytoskeleton as the cells form filopodia.
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  • 120
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    Cell Motility and the Cytoskeleton 1 (1981), S. 205-235 
    ISSN: 0886-1544
    Keywords: capping of receptors ; cell locomotion ; cell-surface interactions ; frictional force ; membrane flow ; polymorphonuclear leukocytes ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: As a cell moves over a surface, the distribution of membrane proteins that adhere to the surface will be changed relative to the distribution of these molecules on a static cell. Observations of this redistribution offer, in principle, evidence as to the mechanisms of membrane dynamics during cell locomotion. Toward extracting such information we present and analyze a mathematical model of receptor transport in the membrane by diffusion and convection, as affected by the making and breaking of the bonds between the receptors and the surface as the cell moves.We show that the disruption of receptor-surface bonds at the tail of the cell provides a mechanism by which the frictional force opposing a cell's motion is exerted, and calculate the magnitude of this force as a function of cell velocity. Assuming this to be the major contribution to the frictional force, we show that when the shear force on a cell is above a critical value it is no longer possible for the cell to slide across the surface. For such large forces, it is still possible for the cell to roll; alternatively the cell can be torn free of the surface.Our analysis of existing data on movement of polymorphonuclear leukocytes indicates that cell motion is not accompanied by a bulk flow of membrane from the front to the back of the cell. The data also indicate that cells do not tend to roll as they move over a surface under normal conditions. The data are most consistent with a model where the membrane as a whole is stationary but where receptors that bind to the surface become coupled to sub-membrane contractile proteins.
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  • 121
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    Cell Motility and the Cytoskeleton 1 (1981), S. 269-272 
    ISSN: 0886-1544
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
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  • 122
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    Cell Motility and the Cytoskeleton 1 (1981), S. 261-268 
    ISSN: 0886-1544
    Keywords: Tetrahymena ; chemotaxis ; temporal-gradient sensing ; modulation of turning frequency ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The motility pattern of Tetrahymena thermophila in a homogeneous attractant field consists of successive “runs” and “turns.” The turning frequency decreases or increases upon an abrupt increase in attractant or repellent concentration, respectively. The dose-response curve for leucine and methionine yields a saturation curve with half maximum modulation of the turning frequency at a concentration of 15 μM and 2 μM, respectively. The turning frequency is modulated at a threshold concentration of 0.02 μM and 0.50 μM for leucine and methionine, respectively. The decrease (increase) in turning frequency in the presence of an attractant (repellent) jump reverts to prestimulus frequency in a time proportional to the concentration jump. Hence, Tetrahymena seem to employ temporal-gradient sensing for chemotaxis. Spatial-gradient taxis is thus exerted by random walk, which is biased in the direction of the gradient.
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  • 123
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    Cell Motility and the Cytoskeleton 1 (1981), S. 237-245 
    ISSN: 0886-1544
    Keywords: centrioles ; symmetry ; triplet blades ; thermal fluctuations ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The paper suggests several principles of construction of a microscopically small device for locating the directions of signal sources in microscopic dimensions. It appears that the simplest and smallest device that is compatible with the scrambling influence of thermal fluctuations as are demonstrated by Brownian motion is a pair of cylinders oriented at right angles to each other. Nine equally spaced blades run in a pitched fashion along the mantle of each cylinder. The blades have a concave cross-section and bend around the circumference of the cylinder in a certain rotational pattern. Considering the striking similarity of this hypothetical device with centrioles, the paper puts forward the conjecture that centrioles locate the direction of hypothetical signals inside cells.
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  • 124
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    Cell Motility and the Cytoskeleton 1 (1981), S. 247-260 
    ISSN: 0886-1544
    Keywords: cilia ; trachea ; ATP-reactivation ; ciliary activity ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Evidence for active sliding of microtubules during ciliary activity has been demonstrated in a number of organisms: sea urchin sperm flagella, protozoan cilia, and mollusc gill cilia. Although there is evidence that active sliding also occurs in mammalian sperm flagella, there is little or no information on whether active sliding of microtubules also occurs in the short (5-μm) cilia of the mammalian trachea or oviduct. Since these cilia are important in tracheobronchial clearance and ovum transport, respectively, it has been important to demonstrate that microtubule sliding is also involved in the activity of somatic cilia. Ciliated apical portions (cortices) and cilia were isolated from rabbit trachea and oviduct, using Triton X-100 to demembranate the cilia. Most of the ciliated cortices reactivated upon addition of ATP, whereas isolated cilia reactivated to a lesser extent. When preparations of cilia were digested with trypsin before or after ATP addition, disintegration of axonemal doublets occurred with about the same frequency as reactivation. These events were recorded using Nomarski optics and dark-field microscopy. When isolated cilia which had been digested by trypsin and exposed to ATP were also prepared for electron microscopy by negative staining, telescoping of doublet microtubules from axonemes could be shown. These results demonstrate that mammalian somatic ciliary doublet microtubules actively slide in a manner similar to that described for invertebrate cilia.
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    Cell Motility and the Cytoskeleton 1 (1981), S. 273-273 
    ISSN: 0886-1544
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
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  • 126
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    Cell Motility and the Cytoskeleton 1 (1981) 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
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  • 127
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    Cell Motility and the Cytoskeleton 1 (1981), S. 371-385 
    ISSN: 0886-1544
    Keywords: rotating filaments ; cytoplasmic streaming ; Nitella ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Our knowledge about the actin-containing characean filaments on the basis of light and electron microscopical investigations is reviewed. Dynamic filamentous networks, known already from isolated droplets, were detected in Nitella rhizoidal cells using light microscopical techniques. Earlier light microscopic observations in cytoplasmic droplets are confirmed and complemented by new model experiments with rotating helices. The motile phenomena occurring at the filament bundles (ring formation, wave propagation, particle translocation, net dynamics, rolling motions, formation of side arms) can, in this way, be imitated in detail. Thus, the concept of cytoplasmic streaming as a translocation along bundles of rapidly rotating helical filaments is supported. In order to explain unidirectional cytoplasmic streaming, a periodic winding up and unwinding of fine filaments is postulated by which ions are periodically bound and displaced. The formation of side arms which is favored during unwinding results in a screw-mechanical different behavior of the filaments in the two directions of rotation and therefore causes permanent particle transport in one direction.
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  • 128
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    Cell Motility and the Cytoskeleton 1 (1981), S. 387-397 
    ISSN: 0886-1544
    Keywords: birefringence ; polarizing microscope ; sea urchin egg ; cortex ; mitosis ; cleavage ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Birefringence (BR) at the cell surface of fertilized eggs of the sand-dollar, Clypeaster japonicus, during mitosis and cleavage was determined with a photoelectric BR detection apparatus [Hiramoto et al, 1981a]. The cortex of about 2 μm thickness is birefringent positive with respect to the normal to the cell surface. The hyaline layer is negatively birefringent. The halo-layer consisting of a row of microvilli surrounding the egg is positively birefringent in normal Ca-free sea water, while it is negatively birefringent in Ca-free sea water with high refractive index. The BR of the cortex gradually increases over the entire surface during mitosis until the onset of cleavage. The BR of the cortex at the polar region reaches a maximum shortly after the onset of cleavage and then decreases, while the BR of the cortex at the equatorial region begins to decrease shortly before the onset of cleavage, reaches a minimum shortly after the cleavage starts, and then increases again as the cleavage furrow advances. The coefficient of birefringence of the cortex is about 2.5 × 10-5 at the maximum. The BR change of the cortex during mitosis and cleavage is interpreted as a passive deformation caused by the constriction of the contractile ring as well as an active structural change of the cortex occurring in the dividing cell.
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  • 129
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    Cell Motility and the Cytoskeleton 1 (1981), S. 399-416 
    ISSN: 0886-1544
    Keywords: myosin heavy chain ; avian muscular dystrophy ; adult and embryonic fast white fibers ; slow red fiber ; rod ; subfragment-1 ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Avian muscular dystrophy is characterized by the degeneration of fast white skeletal muscle fibers, with onset during development. Using a one-dimensional peptide mapping technique, we have detected two forms of the myosin heavy chain in the fast white fibers of adult domestic chickens, one form characteristic of birds homozygous for muscular dystrophy, the other of their normal controls. Four dystrophic strains carrying the same gene for muscular dystrophy were examined.No differences were detected in the embryonic heavy chain peptide maps of normal and dystrophic chickens, consistent with the developmental onset of the condition. Differences were also absent from the peptide maps of heavy chains from slow red fibers, which are unaffected in dystrophy. No dystrophy-specific peptide map differences were detected in the three light chains. Analysis of peptide maps of rod and the heavy chain component of subfragment-1 from normal and dystrophic heavy chains indicates the presence of amino acid sequence differences in the two proteins.
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  • 130
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    Cell Motility and the Cytoskeleton 1 (1981), S. 417-431 
    ISSN: 0886-1544
    Keywords: spindle poles ; centrioles ; cell center ; scaffold ; electron microscopy ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: I have used fluorescence microscopy and antibodies to 10nm filaments and tubulin labelled with contrasting fluorochromes to compare the distribution of these proteins in endothelial cells during cell division. During interphase the two filament systems have entirely different distributions: The bulk of the 10nm filaments form a ring that surrounds the cell center and nucleus and remains parallel to the substrate, while the microtubules radiate from the cell center to the cell's border. When the mitotic spindle replaces the radial microtubule pattern in mitosis, the spindle poles remain within - and in close proximity to - the ring of 10nm filaments. This was confirmed by electron microscopy which showed the ring and centrioles in the same plane separated by a distance of 300-400 nm.
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  • 131
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    Cell Motility and the Cytoskeleton 1 (1981), S. 303-327 
    ISSN: 0886-1544
    Keywords: cilia ; microtubules ; ATPase ; vanadate ; geometry of sliding ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A dynein arm attachment cycle produces sliding between adjacent doublet microtubules (N and N + 1) of cilia. In intact axonemes, in the absence of ATP, almost all arms appear attached at both ends (rigor). When ATP is added, most arms detach from doublet N + 1. In ATP and vanadate, the arms do not return to rigor, suggesting that ATP hydrolysis is required for re-extension and reattachment of the dynein arm, but not for detachment. Using solutions containing dynein to decorate dynein-less axonemal doublets, we confirm this interpretation. In the absence of ATP, both sides of each doublet decorate with arms. Addition of ATP, ATP and vanadate or AMP-PNP causes immediate arm detachment, but only in the first instance, where extensive ATP hydrolysis can occur, does decoration eventually reappear. Dynein decorates heterologous axonemal doublets and brain microtubules, as well as homologous doublets, suggesting that this mechanochemical cycle may have general applicability in microtubule-based cell motility.
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  • 132
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    Keywords: videomicroscopy ; differential interference microscopy ; streaming ; reticulopodial motility ; Allogromia ; microtubules ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: A new method called Allen Video-enhanced Contrast, Differential Interference Contrast (AVEC-DIC) microscopy is shown to be sufficiently sensitive to detect several new features of microtubule-related motility in the reticulopodial network of the foraminifer, Allogromia. The method takes advantage of the variable gain and offset features of a binary video camera to operate the DIC microscope under conditions highly favorable for video imaging, but in which the optical image is virtually invisible to the eye yet retains its full information when viewed by a suitable video camera. The improvements are made possible by setting a dé Senarmont compensator to λ/9-λ/4 at maximal working aperture of internally corrected planapochromatic objectives. Under these conditions, the offset feature of the video camera can reject so much stray light from the instrument and specimen that contrast compares favorably with that observed in high-extinction images, and polarizing rectifiers offer scarcely any advantage. Freed from the constraints of the light-limited conditions of DIC microscopy, video images can be recorded 60 times per second, or over 1,000 times the rate of photomicrographs at comparable magnifications under high-extinction conditions.Application of this method to the reticulopodial network of Allogromia has shown that cytoplasmic organelles are translocated only in contact with single microtubules or bundles of microtubules, and that these organelles fail to move when separated from microtubules. Microtubules themselves undergo both axial translatory (“sliding”) and lateral “zipping and unzipping” movements that have been suggested to occur during mitosis and other biological processes.
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    Cell Motility and the Cytoskeleton 1 (1981), S. 445-454 
    ISSN: 0886-1544
    Keywords: taxol ; microtubules ; polymerization ; tubulin ; mitotic inhibitor ; protein self-assembly ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Dissociated bovine brain microtubule protein has been shown to reassemble at 0°C in the presence of the drug taxol. Tubulin polymerization was monitored both by electron microscopy of the polymeric structures and by incorporation of tritiated GTP into filterable polymeric structures. Most of the labeled guanine nucleotide uptake into tubulin polymeric structures occurred in the first 30 minutes of incubation with the drug. The initial polymerization event results in the formation of protofilamentous tubulin ribbons. The first microtubules were noted after 1 hour of incubation with the drug. After 20 hours of incubation at 0°C with taxol, the bulk of the polymerized tubulin appeared to be in the form of microtubules. Cold-stable tubulin rings with a mean diameter of 34 nm were present in the reaction mixture before the addition of taxol and throughout the 20-hour incubation. Most of the rings were apparantly not involved in the taxol-induced microtubule assembly. The results are consistant with a model whereby taxol induces an initial formation of protofilamentous ribbon structures, mostly from free tubulin dimers, and a slower subsequent folding of the ribbon structures into microtubules.
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  • 134
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    Keywords: videomicroscopy ; polarization microscopy ; streaming ; reticulopodial motility ; Allogromia ; microtubules ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: A new method is described for recording rapid processes of cell motility in polarized light. The Allen video-enhanced contrast (AVEC-POL) method of polarization microscopy achieves significant improvements in resolution, contrast, and the visibility of fine detail by a combination of novel adjustments to a standard (unrectified) polarizing microscope and video camera. Using the full working aperture of a high-power planapochromatic objective lens and compensator setting of λ/9-λ/4, visible images appear lacking in contrast. However, the same images viewed with an appropriate video camera equipped with an electronic offset adjustment can be made to appear with as much contrast as desired, revealing a significantly greater amount of fine detail in the image than can be seen by high extinction visual microscopy alone. At bias retardations between one-ninth and one-quarter wave, the diffraction anomaly observed near extinction disappears. Consequently, polarizing rectifiers are not required with the AVEC-POL method, and images previously requiring photographic exposures of around 20 seconds are sufficiently bright to be registered on the video monitor in 1/60 second. Using an intensity monitor, quantitative measurements of cellular birefringence can be retrieved from live or videotaped images displaying a linear relationship between contrast and phase retardation due to birefringence. The AVEC-POL method also renders accessible to polarized light analysis a number of objects that scatter or depolarize too much light to be studied by high extinction methods. The method is demonstrated on model objects and applied to the highly motile reticulopodial network of Allogromia laticollaris. Rapid motion in close association with microtubules can now be analyzed in greater detail at a significant reduction in the cost of recording.
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    Cell Motility and the Cytoskeleton 1 (1981), S. 329-347 
    ISSN: 0886-1544
    Keywords: actin ; microfilaments ; heavy meromyosin ; mammary gland ; secretion ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Cytochalasin B, a microfilament-altering drug, inhibits lactose synthesis in lactating guinea pig mammary gland [Biochim. Biophys. Acta 392:20, 1975] but not primarily by inhibiting glucose transport [Eur. J. Cell Biol. 20:150, 1979]. In order to study the possible role of microfilaments in lactose synthesis and secretion, we isolated both the alveolar (milk-secreting) and myoepithelial (contractile) cells from lactating mammary gland. Light microscopy shows that the alveolar cell fraction (viability approximately 71%) is homogenous and that the cells retain strong polarity of secretory structures in the apical region. Two proteins were extracted from the alveolar cell fraction. One (mol wt 42,000) comigrates with skeletal muscle actin on SDS-PAGE gels. The other, a high-molecular-weight (180,000) protein (HMWP) may be analogous to actin-binding protein or clathrin. An extract from the myoepithelial cell fraction also contains a protein that comigrates with actin but no HMWP. Whole tissue extract contains the 42K protein, and a 185K HMWP. Examination of the alveolar cell extract by electron microscopic (EM) negative staining revealed meshworks of multistranded, interconnecting filaments, with attached globular structures (100-200 A) (possibly the HMWP) and single filaments (40-60 A diameter) branching off. To localize these filamentous structures in situ, whole tissue was glycerinated and incubated with rabbit skeletal muscle heavy meromyosin (HMM). Masses of filaments in myoepithelial cells served as convenient standards for HMM decoration. Decorated filaments have cross-arms or projections, unlike the narrow, smooth filaments of control tissue. Decorated filaments in alveolar cells are located beneath the plasma membrane, in close association with secretory vacuoles, and near the Golgi apparatus; filaments near the latter two are often oriented perpendicular to the plasma membrane. Microvesicles are embedded in meshworks under the plasmalemma and near the Golgi apparatus. Intermediate-sized (85-115 A diameter), non-decorated filaments diverge from the meshworks of decorated filaments. Microvesicles are associated with intermediate-sized filaments as well. The association of actin-like filaments with secretory vacuoles and microvesicles and their location in areas of the cell concerned with biosynthetic activities suggest a possible function in the intracellular transport of secretory products.
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    Cell Motility and the Cytoskeleton 1 (1981), S. 433-443 
    ISSN: 0886-1544
    Keywords: Physarum ; acellular slime mold ; calcium ion ; calcium-ionophore ; cytoplasmic contraction ; oscillation ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Calcium is now generally thought to play a key role in regulating a variety of cellular movements. When the plasmodium of Physarum polycephalum was treated with the calcium-ionophore A23187 or the quasi-ionophore amphotericin B, Ca2+ leaked out. Ca2+ efflux into the ambient solution from the plasmodial strand segment was measured by the luminescence of a photoprotein aequorin, and the tensile force production was recorded simultaneously. Ca2+ efflux oscillated with the same period as the cycle of tension generation in the strand, but the phase of cyclic changes in Ca2+ efflux was opposite to that of tension generation. That is, Ca2+ efflux fell in the increasing tension phase and rose in the decreasing tension phase. Cyclic changes in efflux of Ca2+ are provisionally interpreted as reflecting corresponding changes in concentrations of free Ca2+ in the cytoplasm.
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    Cell Motility and the Cytoskeleton 1 (1981), S. 469-483 
    ISSN: 0886-1544
    Keywords: microtubules ; nucleation ; mitosis ; nocodazole ; immunocytochemistry ; Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The reassembly of microtubules is described in mitotic cells after release from nocodazole-induced block. The formation of microtubules was followed by light microscopic immunocytochemical staining using the PAP method, combined with to-luidine blue staining of the chromatin. The light microscopic observations on whole cells were compared with ultrastructural observations on thin sections. This step is essential to ascertain complete destruction of microtubules during the nocodazole treatment and to correlate immunocytochemical staining with the presence of microtubules.Removal of nocodazole (10 or 1 μg/ml) after a sufficiently long incubation to induce a complete disappearance of microtubules resulted in the appearance of tubulin staining specifically associated with the centromeres and with one or two isolated points in the cytoplasm. Electron microscopy confirmed that the staining was due to the massive accumulation of small microtubules at the kinetochores and centrosomes. Kinetochore nucleation was seen only in association with condensed metaphase-stage chromosomes and not with the less-condensed prophase chromosomes.In a second type of experiment cells were allowed to enter mitosis in the presence of an incompletely active concentration of nocodazole (0.1 μg/ml). The construction of the mitotic spindle was arrested; however, short microtubules were assembled at the kinetochores and centrosomes.These experiments demonstrate that in living mitotic PTK2 cells the kinetochores, as well as the centrosomes, exert a nucleating action on tubulin assembly.The further elongation of microtubules after removal of nocodazole was seen to occur preferentially along axes between the centrosomes and the kinetochores. This resulted in the construction of normal metaphases that evolved through anaphase and telophase. We have attempted to formulate a hypothesis that may explain the oriented assembly that seems to be essential in the construction of the spindle.
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  • 138
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A technique is developed whereby the large heavily yolk-laden grasshopper egg may be sectioned for cytological study. Eggs of Chortophaga viridifasciata and Circotettix verruculatus were examined.In C. viridifasciata the structure of the late ovarian nucleus and the chromosomes in meiosis, fertilization, and early cleavage are described. In the first maturation twelve rod-shaped tetrads are found. Near the caudal end of the egg a polar body is given off, and in the second maturation division there are twelve dyads. At fertilization, twelve separated vesicles of the female pronucleus are seen scattered about a male pronucleus in which the chromosomes are in prophase.In early cleavage the chromosome numbers in the metaphase are found to differentiate the male- and female-producing eggs. In the former there are twenty-three and in the latter twenty-four chromosomes. A vesicular condition in which the chromosomes retain their boundaries in interkinesis is indicative of chromosome individuality.A comparison of the first and second maturation metaphase chromosomes of the oocyte with those of the spermatocyte shows a similar compact group on the spindle, a likeness in size seriation, and a similarity of form. They differ in that in the spermatocyte complex there are eleven tetrads and one dyad. This latter, the unpaired sex chromosome, falls among the large chromosomes.A comparison of the chromosome complex of Chortophaga viridifasciata with that of Circotettix verruculatus indicates constancy of generic differences in form, size, number, and behavior of the chromosomes.
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    Journal of Morphology 45 (1928), S. 233-257 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The cytological and histochemical evidence presented in this paper shows the following facts: (1) The mitochondria are not directly transformed into yolk. They may be concerned in its synthesis in so far as they are a factor in the interacting cytoplasmic system, but there are no visible morphological expressions of this functioning. (2) The accumulations of aequeous substances in the cytoplasm in the form of droplets stainable vitally by neutral red, ‘vacuoles,’ are the forerunners of the first yolk. During the building up of the yolk the aequeous droplets become more and more dehydrated and lose their capacity for being vitally stained. (3) These aequeous vacuoles give the impregnation results commonly ascribed to the Golgi apparatus and are interpreted as such. Special emphasis is laid on the question of the identification of the Golgi apparatus. (4) The fat arises de novo in the cell independently of the mitochondria or the vacuoles. It becomes dispersed throughout the cell among the yolk plates, and the lipoidal content of the latter increases at the end of vitellogenesis, when the fatty globules are intimately pressed in among the yolk plates. (5) At a late stage in the growth of the yolk plates there is the sudden appearance of large quantities of glycogen in the perinuclear zone and throughout the cytoplasm.
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    Journal of Morphology 45 (1928), S. 441-471 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The septomaxillary bone is described in the various families of the urodeles in which it occurs. Its identity as a hitherto unrecognized cartilage bone of the skull is established and its close relationship with the nasal muscles followed through the group. Its presence or absence, which is found to be another criterion for the recent classification of the urodeles as proposed by Dunn and Higgins, is correlated with the development of the accessory dilatator muscle, since it occurs only in groups where this muscle attains an appreciable size.
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    Journal of Morphology 45 (1928), S. 537-554 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Modifications of the gastro-intestinal tract of Nereis virens are two dorsolateral esophageal cecums, directed anteriorly and posteriorly from the point of attachment. The cecal and esophageal lumina are continuous. Each cecum is composed of acini, possessing a row of elongated cells, basement membrane, and intima. Fibro-elastic tissue is present. The elongated cells contain glycogen and fat.The hepatopancreas of Asterias vulgaris consists of two lobes in each ray, and the numerous acini empty into the bifurcated hepatopancreatic duct which leads into the pyloric stomach. The hepatic cells are columnar and contain glycogen and fat. Pancreatic cells lie in the midregion of the acini.The lobulate liver of Loligo pealii lies on the ventral surface of the duodenum. A capsular membrane envelops the organ. The acini possess basement membrane, columnar cells, and intima; their lumina anastomose, forming a common duct, which leads into the blind sac. The hepatic cells contain fat.The hepatopancreas of Melanoplus femur-rubrum consists of six cecums histologically continuous with the digestive epithelium of the pyloric stomach. Each cecum consists of simple and compound acini which empty into a duct leading to the pyloric stomach. Each acinus possesses basement membrane, columnar hepatic cells, pancreatic cells, and intima. The pancreatic cells occur in islets, usually adjacent to the basement membrane. The hepatic cells contain glycogen and fat.
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    Journal of Morphology 46 (1928) 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
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    The breeding season of the opossum (Didelphis virginiana) and the rate of intrauterine and postnatal developmentThe work on the opossum was begun at the suggestion of Dr. J. T. Patterson, Professor of Zoölogy, the University of Texas, about 1913. It was prosecuted intensively through the generous financial aid and moral support of The Wistar Institute, Dr. M. J. Greenman, Director, and with the assistance of Dr. C. H. Heuser, then fellow of The Wistar Institute. It is to the skill of Doctor Heuser that most of the photographs presented in the four plates accompanying this article and former papers of this series are due. Some of the photographs were taken from fresh living material in January and February of 1917 at Austin, Texas. The embryological investigations soon gave way in large measure to physiological studies in which the following generously aided: Mr. H. A. Wrocbanker, and Mr. Herman Becker, merchant, Austin, Texas; the University of Texas, Department of Zoölogy; The Bache Fund of the National Academy of Science. I take this opportunity of reiterating my indebtedness to these sources of the necessary nervus rerun to carry on the work and for the spirit of helpfulness in which the grants were made. The Wistar Institute is the repository of most of the material collected and will supervise its study in the future. The present writer can primsie only two more installmetns of these ‘studies’: one on the origin of the mesoderm and the chorda dorsalis, the other on pathological ova of the primitive-streak stage and earlier. (1928)
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    Journal of Morphology 46 (1928), S. 143-215 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The breeding season of the opossum at Austin, Texas, begins in January, following a three months' anoestrous period. The modal point for ovulation days is reached in the third week. The rate of intra-uterine development was investigated chiefly by surgical removal of one uterus, noting the stage attained by the ova therein and allowing the surviving uterus to incubate its ova a precalculated period of time. Unique charts epitomize the results. The primitive-streak stage is completed, the medullary groove and chorda begin at seven and one-half days post coitum, seven days post ovulationem, leaving only five and one-half days' actual development of the embryo to birth. The rate of development is compared with Eutherian mammals.The curve of postnatal growth has the shape of embryonic growth curve of higher mammals. The eyes and lips open, at about fifty days (young the size of mice). At this time the young leave the teat for the first time, but are not weaned for about thirty days more. Soon after weaning, the mother may become pregnant again. At ninety to one hundred days (young size of large rats), the young may begin to shift for themselves.
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    Journal of Morphology 45 (1928), S. 1-45 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Five characters-flatness, elongation, posterior pointedness, delay in division of the body, delay in completion of mitosis-are so distributed among the subdivisions of the Opalinidae as to involve either repeated fortuitous appearances of these characters, a thing not to be believed, or trends resident in the germ plasm. The Ophryoscolecidae show similar distribution of two sets of characters. In the Salpidae there is evidence of trends toward: coiling of the gut; decrease in number, size, regularity, and symmetry of body muscles; simplification and degeneration of the eyes. These qualities appear first in the phylogeny in the chain Salpas, the final phase in the life-cycle. In the course of the evolution the solitary Salpas become more and more modified in the same directions, until, in the most highly modified species, the muscles and eyes are as much modified in the solitary Salpas as in the aggregated. These changes, not disadvantageous but rather adaptive in their beginning in the colonial individuals, are harmful to the solitary Salpas, yet the degeneration is, by precocious development, thrown back onto the earlier phase of the life-cycle, the solitary stage. Precocious development is not purely utilitarian, but may be more fundamental, biological.Evolution is discussed in terms of trends resident in the germ plasm, their origin in connection with mutations, their growth, decrease, disappearance, branching; auto-evolution. Discussion is from the standpoint of the germinal stream, internal factors of evolution being emphasized.
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  • 145
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    Journal of Morphology 45 (1928), S. 187-207 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: This paper deals with the description of an organism, externally resembling Müller's larva among the polyclads, collected in the plankton of Monterey Bay, California, during the spring and summer months. Internally, the organization of the various systems is unique. In some respects it bears a certain resemblance to a polyclad in early stages of development; in other regards it approaches the rhabdoceles, more particularly the Acoela. Its more exact relationships are obscured by the fact that, although the larger specimens are not more than 0.7 mm. in length, the sex organs, both male and female, are fully developed and functional. Furthermore, the plan of these systems is unlike that of any turbellarian hitherto described. Whether this organism represents a case of paedogenesis or is a fully developed adult is unknown at present, but in any event the various systems are described and an attempt is made to give it a fairly definite systematic position.
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  • 146
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    Journal of Morphology 45 (1928), S. 259-292 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The digital disks of the tree frogs are covered ventrally with a superficial layer of vertically elongated, fibrous, and distally free cells between which there empty a series of convoluted mucous glands. The latter are surrounded and, in the larger-disked species, divided into blocks by sheets of collagenous fibers. The glands are emptied by the squeezing together of the collagenous fibers when the body weight exerts a pull on the terminal phalanges. The disks function by friction, cohesion, and adhesion.The digital-disk apparatus was fully established before certain groups of frogs became arboreal. It is retained in others which have reverted to the terrestrial habit. The intercalary cartilage increases the efficiency of the apparatus. It did not arise in phylogeny until after the apparatus was developed.As the digital disks vary in extent in both arboreal and terrestrial species, arborealism seems to have resulted from a chance occurrence of large disks in the smaller-bodied forms; at least, there is no progressive modification of the digits toward particular habitat requirements.The subarticular tubercles of many Salientia develop typical climbing apparatus. This may or may not be correlated with an arboreal habit. In the species with the largest subarticular tubercles no apparatus is present. Arboreal salamanders exhibit no special climbing mechanism, but adhere by pressing their moist integument against the substratum.
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  • 147
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    Journal of Morphology 45 (1928), S. 505-535 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: The relation of the hepatopancreas to the pyloric stomach and its physiologic histology were studied. The organ consists of two lobes, united ventrally, which lie along the cardiac and pyloric stomachs and the intestine. Primary and secondary lobulations (acini) are present, and the entire organ is a system of anastomosing tubules (lumina). The tubules progressively converge and form a duct in each lobe which leads ventrolaterally into the pyloric stomach.Each acinus consists of a single layer of hepatic and pancreatic cells supported by a basement membrane, and the interacinar spaces are the seat of fibro-elastic tissue and phagocytic cells. The supporting tissue is laminar with that of the stomach. The arterial capillaries, composed of endothelial cells, lie in the interacinar spaces. The blood supply is by way of the basement membrane to the cells.The hepatic cells of specimens collected in September bear little fat; cells of June specimens are laden with fat. Glycogen is deposited in the hepatic cells; there is no difference between the quantity found in September and in June. The hepatic cells probably contain biliverdin. Chemical analysis indicates the presence of trypsin, amylase, and lipase.A review of the literature and a discussion of homologies with reference to the vertebrate liver are given.
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  • 148
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    Journal of Morphology 45 (1928), S. 599-613 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: A study of spermatogenesis was made on four groups of a pedigreed strain of the moth Philosamia cynthia, and the results were compared with spermatogenesis in the wild material.Deviations from the normal number of chromosomes were observed in two groups. In some individuals the haploid number was 12 instead of 13. Giant spermatocytes were also observed with twenty-four chromosomes. In other individuals, two haploid numbers, 13 and 14, occurred in the same testis.The twelve-chromosome condition is due to linkage of two chromosomes during the late prophase of the primary spermatocyte. The double chromosomes thus formed appeared to divide equally in both divisions. The origin of the fourteenth chromosome was not determined.Correlation of the genetic and cytological data indicates the restriction of aberrant chromosome complexes to two of the four groups and the regularity of the twelve-chromosome complex in certain families, suggesting the conclusion that a new strain arose in the pedigreed material with regard to chromosome variability and that the twelve-chromosome condition is a well-defined characteristic, partly established in some families and probably fully established in others.
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  • 149
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    Notes: The spermatogenesis of five guinea-pigs was studied. The spermatogonial chromosome number is approximately sixty-two plus or minus two. The primary spermatocyte number is approximately thirty-one. The spermatogonial number in the early prophase is lower than it is in later stages. This condition is due to late fragmentation of the large chromosomes found in the earlier stage. A possible sex chromosome of the X-Y type may be identified. Its components segregate during the first maturation division.
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  • 150
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    Journal of Morphology 46 (1928), S. 217-239 
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    Notes: Notwithstanding the fact that several species of Bruchidae have been used by geneticists for several years, no cytological studies have as yet been made on any member of this family of the Coleoptera. The present paper gives a general account of the spermatogenesis of Bruchus quadrimaculatus Fabr.The spermatogonia undergo two mitotic divisions. After the second division, the nuclei remain small and very dense for some time before the beginning of the growth phase. During this interval the nuclei do not assume again the characteristics of the interkinesis stages. In the primary spermatocytes typical tetrads are formed. The chromosomes are asymmetrically V-shaped. The end of one arm of the ‘V’ fuses with the end of the corresponding arm of its synaptic mate. Disjunction takes place in the primary spermatocyte division. After the division of the secondary spermatocytes, the chromosomes become vesicular and form a reticular nucleus in the spermatid, after which the chromatin becomes deposited as a chromatin rim around the nuclear periphery. The diploid number of chromosomes is nineteen in spermatogonia and in male somatic cells, and twenty in female somatic cells. An unpaired X chromosome is present in the spermatogonia, which fails to divide in the primary spermatocyte division, but passes as a whole to one pole in advance of the autosomes. The X chromosome divides normally in the secondary spermatocyte division with the autosomes.In the method of sex determination, Bruchus does not follow the method of the majority of beetles, since most of those studied adhere to the X-Y type.
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  • 151
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    Journal of Morphology 167 (1981) 
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  • 152
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    Journal of Morphology 167 (1981), S. 13-34 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
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    Notes: The structure of the kidney of Leptonychotes weddelli was examined using corrosion casts, India ink injection, and histological methods. Some observations were made on the kidney of the crabeater seal (Lobodon carcinophagus) and the elephant seal (Mirounga leonina). The kidneys in all three species are reniculate, as in many other marine mammalian species. Features that have not been described previously in a phocid seal are a peripyramidal muscle, and venous drainage characterized by a large extrinsic system and a small intrinsic system.Examination of specialized fornices, relative medullary thickness, and the volumes of juxtamedullary relative to peripheral glomeruli (all of which relate to urine concentrating ability) revealed that each reniculus of Leptonychotes is similar to the unilobar kidney of a small mammal that produces only moderately concentrated urine. The high glomerular volume to cortical volume ratio may be related to high glomerular filtration rates after feeding observed in marine mammals.It is concluded that reniculation is more likely to be related to the large size of most marine mammals than to some factor related directly to the marine environment.
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  • 153
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    Journal of Morphology 167 (1981), S. 43-51 
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    Notes: The effects of cold-stress and hibernation on bone dynamics in the femurs of hamsters were investigated using histometric analyses. Control animals were maintained at 27° C for 90 days; experimental animals were kept at 5° C and hibernated for 7, 15, 21, 50, or 90 days. Histometric analyses of cross sections indicated that bone diameter and cortical thickness at the femoral midshaft increased after 83 days of extreme cold and 7 days of hibernation but decreased significantly after 69 days of cold stress and 21 days of hibernation. Osteoporosis was evident although the number of osteons per unit area of bone increased during hibernation. An initial decrease in the number of non-Haversian longitudinal vessels per unit area of bone was seen in experimental animals which was apparently related to a corresponding reduction in cortical thickness. Lacunar area increased in these animals, suggesting that osteocytic osteolysis may be a significant mechanism for calcium regulation during hibernation.
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  • 154
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    Journal of Morphology 167 (1981), S. 65-90 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
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    Notes: Prenatal development of the eye in a microphthalmic hamster strain (“anophthalmic white”) is compared with established normal developmental periods. The mutant eye primordium is first distinguished at an average of ten gestational days (Period 6) by an incompletely invaginated optic cup, uniformly pseudostratified outer neuroepithelial layer and widely separated margins of the optic fissure. The outer layer of the mutant cup subsequently becomes abnormally thickened, especially posteriorly and midventrally, and, except in a few eyes with localized imperfect fusion, the optic fissure is unfused at twelve days (Period 9), by which time fusion is normally complete. At 13 to 15 days (Periods 10-11) the fissure is unfused or irregularly fused in regions of variable location and extent. The occurrence of fissure fusion with concomitant loss of continuity between inner and outer epithelial layers is generally restricted to expanded anterior regions in 14-16 day (Periods 11-12) eyes. The presence of presumptive neural retina in the outer layer of the cup characterizes the mutant eye; and to varying degrees, in day 13-16 eyes, the presumptive neural retina (1) provides persistent continuity between the two cup layers, (2) forms both fused and unfused margins of the optic fissure, and (3) extends into an outer position of the optic cup. As early as 13 days (Period 10), nerve fibers are present in the outer layer of the cup, and by the last prenatal and first postnatal days (Period 12), ectopic nerve fiber bundles are widely distributed.
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  • 155
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    Journal of Morphology 167 (1981), S. 139-165 
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    Notes: The new family Lobatocerebridae, Rieger, contains a group of turbellariomorph worms in the annelid line of evolution. The fine structural organization of the body wall, the digestive tract, and parts of the central and peripheral nervous system are described and the findings are discussed in light of general invertebrate cytology. The epidermis and gastrodermis contain a basal granule cell system which is structurally very similar to the neuroglia cell system of the nervous system. The continuity of the neuroglia cell system, and the epidermal basal granule cell system and the basal granule cell system in the digestive epithelia suggests the existence of a single glial-basal granule cell system, similar to the gliointerstitial cell system first recognized in the Mollusca (see Nicaise, '73). The Annelida may show a dual (ectodermal and mesodermal) origin of such a gliointerstitial cell system as suggested by similarities in the epidermal basal cell system in the Oligochaeta and of certain epidermal and gastrodermal cells in polychaete regeneration with neuroglia in the Annelida. The structural similarity of neuroglia and basal granule cells in Lobatocerebridae may be the result of similarity in the formation, maintenance, or regulation of the extracellular matrix.
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  • 156
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    Journal of Morphology 167 (1981), S. 201-209 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
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    Notes: Eyes of early embryonic chicks possess 14 scleral papillae, derived from the conjuctival epithelium and present as transient structures between seven and 11 days of incubation. These papillae induce the formation of the 14 scleral ossicles, which develop in the adjacent, neural crest-derived ectomesenchyme. Each papilla undergoes a predictable series of developmental changes, divided by Murrary ('43) into six morphological stages (M stages 1-6). We have confirmed his staging, and provide a scanning electron microscopic (SEM) evaluation of papilla development. The earliest stage that can be visualized with the S.E.M. is M stage 2. We describe the initial modifications of the surface of papilla cells, the presence of large microvilli and the asymmetrical morphogenesis and growth of the papillae. Papillae are shed by a mechanism that involves elongation of the cells at the base of the papilla. Such moribund papillae consist of necrotic cells coated with fibers.
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  • 157
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    Journal of Morphology 167 (1981), S. 231-247 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The penetration of the sperm into the egg, and the movements of the male and female pronuclei were followed from sperm attachment through pronuclear fusion, using time-lapse video microscopy of gametes and zygotes of the sea urchin Lytechinus variegatus (23° C). The pronuclei move in four stages: I. Sperm Entry Phase, following sperm-egg fusion and a rapid radiating surface contraction (5.9 ± 1.3 μm/second) when egg microvilli engulf the sperm head, midpiece, and tail to form the fertilization cone and the sperm tail beats in the egg cytoplasm; II. Formation of the Sperm Aster, which pushes the male pronucleus centripetally at a rate of 4.9 ± 1.7 μm/minute starting 4.4 ± 0.5 minutes after sperm-egg fusion, as the male pronucleus undergoes chromatin decondensation; III. Movement of the Female Pronucleus, the greatest and fastest of the pronuclear motions at a rate of 14.6 ± 3.5 μm/minute at 6.8 ± 1.2 minute after sperm-egg fusion, which establishes the contact between the pronuclei; and IV. Centration of the Pronuclei to the egg center at a rate of 2.6 ± 0.9 μm/minute by 14.1 ± 2.6 minutes after sperm-egg fusion. Pronuclear fusion typically occurs after stage IV and proceeds rapidly starting 14.7 ± 3.6 minutes after sperm-egg fusion with the male pronucleus coalescing into the female pronucleus at a rate of 14.2 ± 2.6 μm/minute.
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  • 158
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    Journal of Morphology 167 (1981), S. 297-304 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
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    Notes: Eggs of Chelydra serpentina were incubated at 30°C and 26°C. In addition, incubation was done at 20°C during the temperature-sensitive period for sex determination. Incubation at 20°C and 30°C resulted in females; incubation at 26°C resulted in males in 99% of the cases. The average gonadal length was less in the males. The average length of the 20°C ovaries did not vary significantly from that of the 30°C ovaries.The condition of the oviducts was correlated with histology of the gonads in hatchlings and in 3-month-old animals. When at least one of the oviducts was obvious and intact, ovaries were present. If the oviducts were absent or interrupted, testes were present. Histological characteristics of the gonads resulting from the three incubation temperatures are described. In the 26°C testes, cellular infiltrations occurred frequently. The ovaries of 20°C hatchlings tended to have a less developed germinal epithelium than that of the 30°C animals. Also, epithelial cysts occurred frequently in the 20°C ovaries. The incidence of follicles at 3 months was not differential.
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    Journal of Morphology 168 (1981) 
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  • 160
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    Journal of Morphology 167 (1981), S. 339-375 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
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    Notes: The hemimandibles in carnivorans may be united in various ways at the symphysis menti. The symphysis may contain a readily flexible joint that permits a moderate amount of independent movement of the hemimandibles. This type of symphyseal union is primitive for and widely distributed in extant carnivorans. In other carnivorans, the symphysis is patent but allows slight or essentially no independent movement of the hemimandibles. Finally, the hemimandibles may be rigidly united by synostosis of the symphysis. The morphology, movement and, insofar as possible, function of these types of symphyses are described.
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    Journal of Morphology 168 (1981), S. 5-15 
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    Notes: The lung volume, the morphometrically determined alveolar and capillary surface area, and the capillary volume of 27 dogs (weight 2.65-57 kg) all were linearly correlated with body weight. The thickness of the air-blood barrier increased only slightly with increasing body size. The structural diffusing capacity, containing these parameters, was used to estimate the gas exchange capabilities of the lung and was also found to scale in direct proportion to body size. This coincides with reports on physiologically estimated diffusing capacity but is obviously different from the interspecies slope for metabolism which scales to the 3/4 power of body weight.
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  • 162
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    Journal of Morphology 168 (1981), S. 1-2 
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  • 163
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    Journal of Morphology 168 (1981), S. 43-49 
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    Notes: A realistic model of the distribution of the partially orientated capillaries in skeletal muscles has been introduced for stereological analyses. Distributional parameters not previously estimated for capillary networks in muscles have been quantified. These include the lengths of capillary per unit volume of tissue (Lv) and a dimensionless index of orientation (Ω). The present study demonstrates that surgical techniques for inducing skeletal muscle hypertrophy can be an effective stimulus for the proliferation of additional capillaries. In the hypertrophic muscles studied the capillaries become more highly orientated. This suggests that the growth of new capillaries occurs preferentially along the long axis of the muscle.
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  • 164
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    Journal of Morphology 168 (1981), S. 97-108 
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    Notes: Eggs of a number of cockroach species are parasitized by Tetrastichus hagenowii. The ultrastructure of the sensilla on the antennae of females and males was examined by scanning and transmission electron microscopy. The females have two types of multiporous plate sensilla while the males have only one. Type 1 is found in females and males and has a relatively thin cuticular wall and many pores, while type 2 is found only in females and has a relatively thick cuticular wall and few pores. Both sexes have nonporous, thick-walled, socketed hairs; multiporous, nonsocketed hairs; multiporous, thick-walled pegs; and terminal hairs. In addition, males have multiporous, nonsocketed, long hairs. The sensilla are similar, in many respects, to the sensilla of other chalcid parasitoids. The antennal sensilla of female T. hagenowii are probably involved in ovipositional behavior. The multiporous, long hairs of the male possibly receive stimuli during mating behavior A chemoreceptive function is proposed for the multiporous plate sensilla.
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  • 165
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    Journal of Morphology 168 (1981), S. 137-149 
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    Notes: The ultrastructure of hemopoietic bone marrow of the Spanish lizard, Lacerta hispanica, has been studied for the first time. The organ consists of a stroma formed by venous sinuses and reticular cells. Erythropoiesis takes place in the lumen of blood vessels, while granulopoiesis is extravascular. Pluripotent stem cells are structurally differentiated into erythrocytes and granulocytes. Two types of granulocytes, heterophils and acidophils, have been found, and a third granular cell type is tentatively identified as granular leukocyte. Remarkably, plasmacytopoiesis occurs in the bone marrow of Lacerta hispanica. The possible functional significance of these results is discussed with emphasis on their importance for the reptilian immune system.
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  • 166
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    Journal of Morphology 168 (1981), S. 181-187 
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    Notes: The cells of mandibular organs of female Libinia emarginata exhibit changes in substructure during molting and vitellogenesis. The cytoplasm is that of a steroid-hormone producing cell. The cells do not appear to produce ecdysones.
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  • 167
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    Journal of Morphology 167 (1981), S. 265-276 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Morphogenesis of the eye was studied in a new strain of micro-phthalmic rat. Abnormalities were noted immediately after the formation of the optic cup. The inner layer in the central part of the optic cup was relatively thick and contained many mitotic figures, whereas that of the marginal part was thin and contained only a few. The transitional point in the inner layer between the central and the marginal parts was well marked. This is evidently due to the extreme growth inhibition of the inner layer at the marginal part.At the early developmental stage, an area of the inner layer corresponding to the transitional point protruded toward the lens because the central part of the inner layer continued to differentiate.The differentiation and the protrusion of the inner layer proceeded variably at the later stages depending on the degree of the growth inhibition. The eyes were classified into three groups: Group A-the retina was recognized as a cyst consisting of the pigment layer and the pigment-layerlike structure which originated from the inner layer; group B-the neural retina and its layered structure were inverted; group C-abnormalities, such as the destruction of the lens, were observed.Although previous authors who studied eye mutants suggested the vascular abnormality as the primary cause of the production of abnormal eyes, we feel that this is not the case in our animals.
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  • 168
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    Journal of Morphology 167 (1981), S. 305-312 
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    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The fine structure of Johnston's organ in the pine sawfly, Neodiprion sertifer, was studied by electron microscopy to determine if there exists a dimorphism in this organ corresponding to the sexual dimorphism in antennal shape and surface area.The organ is made up of scolopidia that are ultrastructurally similar to those of other insects. The scolopidia, identical in both sexes, comprise three sensory cells bearing two types of sensory processes: Two are shorter and smaller in diameter than the third, which extends into the cuticle of the membrane connecting pedicel and flagellum and terminates at an epicuticular invagination. The dendrites and sensory processes are surrounded by two types of enveloping (glial) cells-a scolopale cell and an attachment cell. Other enveloping cells occur at different levels of the scolopidium.Sexual dimorphism is evident only in the numbers of scolopidial groups: Males have more groups with fewer scolopidia, but both sexes possess about the same total number of scolopidia.
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  • 169
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    Journal of Morphology 167 (1981), S. 333-337 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A polytrophic ovariole of the flour moth, Ephestia kuhniella, is composed of a linear series of increasingly mature egg chambers, each consisting of an oocyte, an interconnected cluster of seven nurse cells, and a covering layer of follicle cells. This study describes changes in the volume of each component as a function of the position of the egg chamber in the ovariole. Analysis of the growth curve of the Ephestia oocyte yields two possible correlations between accelerated oocyte growth and ultrastructural events enhancing the supply of yolk materials to the oocyte: the first is the initiation of yolk synthesis by the follicle cell layer and its transfer to the oocyte, and the second is the formation of channels between the follicle cells allowing hemolymph to gain access to the oocyte. An Ephestia oocyte increases in volume from approximately 2.5 × 103 μm3 to approximately 2.0 × 107 μm3 over an average series of 58 egg chambers.
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  • 170
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    Journal of Morphology 168 (1981), S. 3-4 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
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  • 171
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    Journal of Morphology 168 (1981), S. 17-42 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Embryonic development of the head of Oxyrhachis tarandus (Membracidae) has been investigated in detail to settle the controversy of head segmentation and to refute the occurrence of an intercalary segment. The head is formed from six distinct elements: the prostominal lobe, the paired cephalic lobes, the antennal segment and the three noncontroversial gnathal segments. The prostomial lobe, which possesses a neuromere and a pair of coelomic cavities, represents the first body segment, called the prostomial segment. The tritocerebral lobes of the brain and the stomatogastric nervous system, consisting of a frontal ganglion, clypeolabral nerves, and the recurrent nerve etc., develop from the neuromere of the prostomial lobe. The tritocerebrum thus belongs to the prostomial segment rather than to an imaginary intercalary segment and mainly represents the ganglionic center of the stomatogastric nervous system in the brain. Frons, clypeus, and labrum develop from the outer wall of the prostomial lobulate plate, whereas the epipharyngeal wall, including the cibarial pump, develops from its inner wall. The presence of three coelomic cavities and of three distinct neural masses in the cephalic lobes during the initial stages of development shows that they have developed by the fusion of three distinct segments during the long phylogenetic history of insects. The portion of the germ band presently considered as the intercalary segment is actually the sternal part of the antennal segment. The neural cells located in this region give rise to the deutocerebrum by shifting forward, around the stomodaeum, and always leaving a commissure behind. The intercalary segment is thus a complete illusion. The antennal segment is postoral in the beginning and bears a pair of coelomic cavities, but later on it shifts forward and its sternal part invaginates into the stomodaeum.
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  • 172
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    Journal of Morphology 169 (1981), S. 1-19 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Hands of two-toed sloths (Choloepus) are long, narrow, hook-like apparatuses with only two functional digits (II and III); rays I and IV are represented only by metacarpals. The proximal phalanges of digits II and III are shortened to essentially proximal and distal articulating surfaces, and all but distal interphalangeal joints of these digits are restricted by interlocking surfaces to minimal ranges of flexion and extension. Several intercarpal joints and the wrist joint, however, allow wide ranges of movement in several axes. Wide excursion at the wrist is permitted by an extremely lax joint capsule, the manner of insertion of several prime movers of the carpus, and the reduced participation of the ulna in the wrist joint. Several extrinsic digital muscles, particularly extensors, are absent and others have unusual actions. Intrinsic musculature consists primarily of mm. interossei and m. extensor digitorum brevis, although other, inconstant muscles do occur. Hands of Choloepus are used as flexible hooks on supports less than 52 mm in diameter and as fixed grapnels on larger supports. In both cases, distal phalanges (and covering claws) form the “hook” element. Whereas bare volar pads seem to be adjunctive on supports smaller than 52 mm in diameter, they are essential on those larger than 65 mm. Two-toed sloths may prefer supports 50 mm in diameter or smaller. The potential importance of vines as supports is discussed.
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  • 173
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    Journal of Morphology 169 (1981), S. 113-140 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: A comparative study of limb morphology indicates that the osteological and myological differences between Didelphis virginiana, the Virginia opossum, and Chironectes minimus, the water opossum, may be associated in Chironectes with decreased resistance to water and increased mechanical advantage of its muscles for increased force. Limb myology is described and a synonymy of terms is applied to the musculature of these two opossums.
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  • 174
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    Journal of Morphology 169 (1981), S. 161-183 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The larval chondrocranium of the pelobatoid anuran Pelodytes punctatus was studied, using Alcian blue-alizarin stained and cleared whole preparations, serial sections, and gross dissections. This beaked tadpole is an unspecialized pond dweller and its chondrocranium closely resembles those of other ecologically similar tadpoles of diverse systematic relationships. Detailed analysis, however, shows many differences among the chondrocrania of anuran larvae. Among other features, these include the configurations of suprarostrals, fronto-parietal fenestrae, palatoquadrate suspensoria, the ligamentum or cartilago tectum of the muscular process of the quadrate and the circumoral ligaments. The lateral circumoral ligament permits differentiation of beaked discoglossoidean and ranoidean larvae. Microhyloids conform to the ranoidean pattern in this feature. Pipoids either lack it or seem to conform to the discoglossoidean pattern. Use of these larval features as key characters enables assignment of Pelodytes to an uncertain position among pelobatoid frogs. This is totally congruent with previous assignments based on adult features and is used to support the hypothesis that anuran phylogenies based on larval characters will closely resemble, in major features at least, those based solely on adult characters.
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  • 175
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    Journal of Morphology 169 (1981), S. 243-251 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The site and process of urine formation in the renopericardial system of Helisoma have been investigated. Osmotic pressure and protein content of hemolymph from the heart, pericardial fluid from the pericardial cavity, prourine from the kidney sac, and urine from the ureter have been determined. Osmotic pressure is equal in hemolymph, pericardial fluid, and prourine, but less in urine. Protein content is similar in hemolymph and pericardial fluid, but much less in prourine and urine. Hemoglobin molecules are present in hemolymph and pericardial fluid but not in prourine. It is suggested that in Helisoma the kidney sac is the site of prourine formation, and prourine is an ultrafiltrate of hemolymph. The kidney epithelial cells contain 6- to 7-nm microfilaments which react with heavy meromyosin producing unidirectional arrowheads. Numerous actin filaments are present in the vicinity of the lateral cell membranes and basal processes. It is possible that the actin filaments regulate the extracellular spaces for prourine passage. It is postulated that the actin-rich kidney epithelium may generate hydrostatic pressure for ultrafiltration. Na+-K+ ATPase is located on the luminal side of the kidney epithelium, which may regulate intracellular fluid level of the kidney epithelial cells, and thereby regulate their cell volume. Thus Na+-K+ ATPase may be involved in the regulation of extracellular spaces in kidney epithelial cells. The enzyme may participate in the production of hyposmotic urine.
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  • 176
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    Journal of Morphology 169 (1981), S. 259-274 
    ISSN: 0362-2525
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The cytoarchitecture and neuromorphology of the torus semicircularis in the tokay gecko, Gekko gecko, were examined in Nissl-stained, fiber-stained, and Golgi-impregnated tissues.From a superficial position, the torus semicircularis extends rostrally under the caudal half of the optic tectum. Caudally, the two tori abut upon one another; rostrally, they diverge. The torus semicircularis consists of central, laminar, and superficial nuclei.The central nucleus consists of fusiform, spherical and triangular neurons. Their dendrites are highly branched, with numerous dendritic spines, and are oriented mediolaterally, dorsoventrally, and rostrocaudally. Fusiform and spherical neurons display two dendritic patterns: “single axis,” ramifying in one axis, and “dual axis,” exhibiting higher-order branches perpendicular to the primary dendrites. Triangular neurons exhibit a “radiate” dendritic pattern.In the rostral half of the torus semicircularis, the laminar nucleus caps the central nucleus. The laminar nucleus encircles the central nucleus in the caudal torus semicircularis. The neurons of the laminar nucleus have dendritic arrays oriented parallel to the border of the central nucleus. These dendrites exhibit a paucity of dendritic spines and higher-order branches. Fusiform and spherical neurons exhibit “single axis” and “dual axis” dendritic patterns. Triangular neurons display “radiate” patterns.The caudal superficial nucleus lies dorsal and dorsolateral to the central nucleus. The superficial nucleus is sparsely populated by small fusiform and spherical neurons with moderately branched dendrites and moderate numbers of dendritic spines. These neurons display “single axis” (fusiform neurons) as well as “dual axis” and “radiate” (spherical neurons) dendritic patterns. They are oriented either parallel to or perpendicular to the boundary of the laminar nucleus.
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  • 177
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    Journal of Morphology 169 (1981), S. 357-357 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
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  • 178
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    Journal of Morphology 170 (1981), S. 43-54 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
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    Notes: Major features of interest in the mature chondrocranium of Agama pallida are striking curvature of the nasal region, lack of the paranasal cartilage and concha nasalis, and presence of a cartilaginous roof over Jacobson's organ. In addition, the course of the ethmoid nerve deviates from the normal lacertilian pattern; there is no foramen epiphaniale, and the temporal region is reduced. The prefacial commissure and facial foramen lie in front of the cochlear portion of the auditory capsule, whereas the prominentia semicircularis anterior is separated from the rest of the otic capsule. Several chondrocranial characters are suggested to be unique to the agamids.
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  • 179
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    Journal of Morphology 170 (1981) 
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  • 180
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    Journal of Cellular Physiology 106 (1981), S. 173-178 
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Proteins of approximately 10,000 daltons (presumably metallothionein) and greater than 75,000 daltons bound 64Cu when this metal was added to fibroblast lysates. Treatment with either 2-mercaptoethanol or the disodium salt of ethylenediamine tetraacetic acid demonstrated that the high molecular weight copper-binding proteins in lysates prepared from both normal and Menkes fibroblasts exhibited a relatively low affinity for copper compared to the 10,000 dalton protein(s). No difference was detected in the affinity of the low molecular weight protein(s) of normal and Menkes fibroblast lysates for copper. The amount of 64Cu bound to the 10,000 dalton protein(s), however, was approximately two to three times greater in lysates prepared from Menkes fibroblasts than from normal fibroblasts. Mixing experiments indicated that the increased binding of 64Cu to the 10,000 dalton protein(s) in lysates of Menkes fibroblasts did not result from the deficiency of a factor that effects the cleavage of copper from this protein(s), from the presence of a soluble inhibitor, or from the lack of an activator. In addition, the use of lysates, rather than whole cells, demonstrated that the observed differences in copper binding between the normal and the Menkes fibroblasts were not caused by an abnormality in the membrane transport of copper in the mutant cells. Thus the findings suggest that the increased accumulation and the reduced efflux of copper previously observed in cultured Menkes fibroblasts result either from an increased amount of the 10,000 dalton copper-binding protein(s) or from an increased capacity of this molecule(s) for copper.
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  • 181
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    Journal of Cellular Physiology 106 (1981), S. 209-213 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Fusion of mononucleate myoblasts to form multinucleated myotubes increases when skeletal muscle cells are grown in progressively higher oxygen concentrations (5%, 20%, and 40% oxygen). At four days of growth fusion of myoblasts (as expressed by the percent of all muscle nuclei that are located in myotubes) is 57 ± 2% in 5% oxygen, 68 ± 1% in 20% oxygen, and 78 ± 2% in 40% oxygen (P〈0.001). However, at a concentration of 40%, oxygen depresses the rate of cell division and thereby affects the number of myoblasts available for fusion. Thus, oxygen concentration significantly modifies growth of skeletal muscle in vitro. Its net effect on myotube formation results from the interaction of its separate effects to enhance cell fusion and to depress cell proliferation.
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  • 182
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    Journal of Cellular Physiology 106 (1981), S. 225-234 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Primary cultures of bone cells and skin fibroblasts were examined for their Ca++ content, intracellular distribution and Ca++ fluxes. Kinetic analysis of 45Ca++ efflux curves indicated the presence of three exchangeable Ca++ compartments which turned over at different rates: a “very fast turnover” (S1), a “fast turnover” (S2), and a “slow turnover” Ca++ pool (S3). S1 was taken to represent extracellular membrane-bound Ca++, S2 represented cytosolic Ca++, and S3 was taken to represent Ca++ sequestered in some intracellular organelles, probably the mitochondria. Bone cells contained about twice the amount of Ca++ as compared with cultured fibroblasts. Most of this extra Ca++ was localized in the “slow turnover” intracellular Ca++ pool (S3). Serum activation caused the following changes in the amount, distribution, and fluxes of Ca++: (1) In both types of cells serum caused an increase in the amount of Ca++ in the “very fast turnover” Ca++ pool, and an increase in the rate constant of 45Ca++ efflux from this pool, indicating a decrease in the strength of Ca++ binding to ligands on cell membranes. (2) In fibroblasts, serum activation also caused a marked decrease in the content of Ca++ in the “slow turnover” Ca++ pool (S3), an increase in the rates of Ca++ efflux from the cells to the medium, and from S3 to S2, as well as a decrease in the rate of influx into S3. (3) In bone cells the amount of Ca++ in S3 remained high in “serum activated” cells, the rate of efflux from S3 to S2 increased, and the rate of influx into S3 also increased. The rate of efflux from the cells to the medium did not change. The results suggest specific properties of bone cells with regard to cell Ca++ presumably connected with their differentiation. Following serum activation we investigated the time course of changes in the amount of exchangeable Ca++ in bone cells and fibroblasts, in parallel with measurements of 3H-thymidine incorporation and cell numbers. Serum activation caused a rapid decrease in the content of cell Ca++ which was followed by a biphasic increase lasting until cell division.
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  • 183
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    Journal of Cellular Physiology 106 (1981), S. 269-277 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: We have shown that collagen gel can be used as a culture matrix for the cloning of granulocyte/macrophage progenitor cells (CFU-C), the production of foci of marrow stromal cells and the maintenance of stem cell proliferation, differentiation and the production of CFU-C. Since collagen is a physiological matrix and allows the simultaneous growth of a variety of cellular elements, the system should prove useful for examining the role of cell/cell interactions and regulatory molecules involved in haemopoiesis.
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  • 184
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    Journal of Cellular Physiology 106 (1981), S. 283-291 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Results of hemacytometer cell counts and of tyrosinase measurements made by the Pomerantz method demonstrate that imidazole added to the medium of cultured B 16 mouse melanoma cells can stimulate tyrosinase specific activity and inhibit cell division. These effects are greater than with adenosine 3′,5′ cyclic monophosphate (cAMP) or the cAMP-phosphodiesterase inhibitor theophylline. The effects of imidazole on cell division and tyrosinase are enhanced by theophylline and antagonized by cAMP. Cyclic AMP-phosphodiesterase activity in cell-free extracts can be inhibited by theophyllne and stimulated by imidazole. However, imidazole does not affect cAMP-phosphodiesterase specific activity in vivo, nor does it affect intracellular cAMP concentrations as determined by competitive protein-binding assays. In contrast, the specific activity of cAMP-phosphodiesterase in vivo is stimulated by cAMP and theophylline, supporting the hypothesis that cAMP and agents which increase intracellular cAMP concentrations induce the synthesis of cAMP-phosphodiesterase. Studies with actinomycin-D and cycloheximide support the hypothesis that cAMP can also mediate posttranslational activation of tyrosinase. Similar experiments suggest that imidazole, or a derivative therof, can induce the synthesis of tyrosinase at the pretranslational level of control. We hypothesize that this type of regulation (pretranslational) by imidazole may define a role for the concept of “Metabolite Gene Regulation” (MGR), in mammalian cells.
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  • 185
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    Journal of Cellular Physiology 106 (1981), S. 399-406 
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The effects of valinomycin (25 pM) on the membrane potential and on initial, passive Na+ and K+ movements have been determined in Ehrlich ascites tumor cells. The membrane potential of steady-state cells in a physiologic environment was - 23.2 mV. Addition of valinomycin induced a small, significant hyperpolarization (Vm = -29.6 mV) when averaged over the population tested. However, analyses of the response of individual cells to valinomycin showed two different potential effects: (1) the majority of cells hyperpolarized after treatment; but (2) a significant fraction depolarized when exposed to valinomycin. The Vm of steady-state cells incubated in saline with K+ at concentrations of 21 mM or 75 mM was - 21.4 mV and -22.0 mV, respectively. Addition of valinomycin to these cells was without effect on Vm, thus establishing the “null point” responses. Only for cells incubated in saline with a K+ of 75 mM was there agreement between Vm and K+ equilibrium potential (Vk). Determinations of cellular Na+ and K+ showed that valinomycin induced net losses of K+ and gains of Na+ by cells incubated in either physiologic saline or saline with a K+ concentration of 21 mM. However, the celular K+ of cells incubated in saline with a K+ concentration of 75 mM was unaltered by valinomycin. There was a two- to threefold increase in K+ permeability of the cell membrane in the presence of valinomycin. These results are consistent with the existence of two null points in the membrane-potential response to valinomycin: One is established when the membrane potential corresponds to Vk; the second occurs when the effects of valinomycin on K+ loss from the cell are exactly offset by its inhibition of active Na+ + K+ transport.
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  • 186
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    Journal of Cellular Physiology 106 (1981), S. 425-434 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The mechanism of glucose entry into human vascular endothelial cells was studied in monolayer cultures of normal (primary) and virally (SV40) transformed umbilical vein endothelium. Radioisotopic uptake studies with the glucose analogues 2-deoxy-D-glucose, and 3-O-methyl-D-glucose, and the nonmetabolizable stereoisomer L-glucose, indicated the presence of a saturable, stereospecific hexose carrier mechanism in both cell types. In other experiments with D-glucose and 3-O-methyl-D-glucose, the phenomenon of countertransport was demonstrable. Hexose transport was not affected by KCN, dinitrophenol, or ouabain, but was inhibited by phloretin and phlorizin in a pattern consistent with facilitated diffusion. Kinetic constants were obtained for both 2-deoxy-D-glucose and 3-O-methyl-D-glucose uptake. Similar Km values (range, 3.3-4.7 mM) were noted with normal and transformed cells, whereas the apparent Vmax was 0.56 nmol/μ1 cytosol/minute for primary cells and 1.7-2.5 nmol/μ cytosol/minute for transformed cells. Under standard culture conditions, as well as following 18 hours of serum deprivation, insulin at concentrations up to 10-5 M did not appear to influence hexose uptake in either cell type. Metabolism of 14C(U)-D-glucose to 14CO2 also was not stimulated by insulin. The presence of an insulin-insensitive, facilitated transport system for glucose in vascular endothelium has relevance for glucose metabolism in this tissue, and potentially for the association of certain vascular diseases (e.g., diabetic microangiopathy, atherosclerosis) with altered glucose homeostasis.
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  • 187
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    Journal of Cellular Physiology 106 (1981), S. 435-444 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Activity and accuracy of chromatin-directed DNA replication have been compared in young and aged Mus musculus and Peromyscus leucopus, two murine species with contrasting maximum lifespans. Chromatin isolated from livers of mature adults of both species copied efficiently exogenous DNA templates using predominantly DNA polymerase-β. The DNA synthetic activity of liver chromatin remained constant in both species throughout their lifetimes. The fidelity of chromatin-directed poly [d(A-T)] synthesis was similar for the comparatively short-lived M. musculus and the relatively long-lived P. leucopus and remained unaltered in old animals. The fidelity of poly [d(A-T)] copying catalyzed by DNA polymerase-β dissociated from liver chromatin was comparable to that of the chromatin-directed synthesis. The dissociated enzymes did not exhibit diminished fidelity of poly [d(A-T)] synthesis with age. In all ages of both species examined, the murine liver DNA polymerase-β, both chromatin-associated and solubilized, exhibited high error frequencies; approximately one dGMP was incorporated for every 500-1,000 complementary nucleotides polymerized. The relationship of these results to the accuracy of DNA replication and repair as a determinant of aging is considered.
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  • 188
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    Journal of Cellular Physiology 106 (1981), S. 419-424 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Exposure of murine leukemia cells in culture to bis-acetyl-diaminopentane (BADP) caused erythroid maturation as measured by the accumulation of hemoglobin in treated cells. The appearance of differentiated cells in cultures exposed to BADP occurred 18 to 20 hours earlier than in those treated with dimethylsulfoxide (DMSO), a standard inducer of differentiation in this system. Studies with [3H]BADP indicated the occurrence of relatively rapid association of the inducer with cells, and subsequent linear accumulation. Fractionation of cellular components and measurement of radioactivity from BADP therein demonstrated that this agent preferentially associates with a fraction enriched for plasma membrane. In addition, [3H]BADP was capable of binding to the plasma membrane-enriched fraction isolated from murine erythroleukemia cells as measured by gel filtration. These findings support the concept that interaction of inducers of murine erythroleukemia differentiation such as BADP with components of the surface membrane may be important in the cascade of events that lead to the erythroid maturation of these leukemic cells.
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  • 189
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    Journal of Cellular Physiology 107 (1981) 
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  • 190
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    Journal of Cellular Physiology 107 (1981), S. 11-19 
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    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Primary cultures of parenchymal cells isolated from adult rat liver by a collagenase perfusion procedure and maintained as a monolayer in a serum-free culture medium were used to study glucoeogenesis and the role that the glucocorticoids play in the control of this pathway. These cells carried out gluconeogenesis from three-carbon precursors (alanine and lactate) in response to glucagon and dexamethasone added alone or in combination. Maximum glucose production was observed with cells pretreated for several hours with dexamethasone and glucagon prior to addition of substrate and glucagon (8- to 12-fold increase over basal glucose production). Half-maximum stimulation of gluconeogenesis was seen with 3.6 × 10-10 M glucagon and 3.6 × 10-8 M dexamethasone. Maximum stimulation was oberved with 10-7 M glucagon and 10-6 M dexamethasone. The length of time of dexamethasone pretreatment was found to be important in demonstrating the effect of glucocorticoids on glucagon-stimulated gluconeogenesis. Treeatment of cells with dexamethasone for 2 hours did not result in an increase in glucose production over identical experimental conditions in the absence of dexamethasone, wherease pretreatment for 5 hours (1.2-fold increase) or 15 hours (1.7-fold increase) did result in an increase in glucose production. The results establish that the adult rat liver parenchymal cells in primary culture are a valid model system to study hepatic gluconeogenesis. In addition, we have established directly that the glucocorticoids amplify the glucagon stimulation of gluconeogenesis.
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    Journal of Cellular Physiology 107 (1981), S. 41-46 
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: An intraperitoneal injection of the beta-adrenergic drug dl-isoproterenol hydrochloride (100 mg/kg body weight) into male (190-210 g) albino rats caused two cyclic AMP surges (peaking at 10 minutes and again between 8 and 12 hours) and the initiation of DNA synthesis (between 16 and 20 hours) in the parotid glands. The parotid cells in hypocalcemic thyroparathyroidectomized rats still responded to isoproterenol injection by generating the two cyclic AMP surges, but they did not initiate DNA synthesis unless a blood calcium-elevating combination of parathyroid hormone (50 USP units/100 g of body weight) and 1α,25 (OH)2 vitamin D3(200 pmoles/100 g of body weight) was injected along with the isoproterenol.
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  • 192
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    Journal of Cellular Physiology 107 (1981), S. 59-67 
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The potent tumor promoter 12-O-tetradecanoyl-phorbol-13-acetate (TPA) can stimulate quiescent, nonproliferating 3T3 cells to reenter the cell cycle and divide. We have previously used a slection technique developed in our laboratory to isolate variant cell lines which no longer divide in response to epidermal growth factor. We have now utilized the same selection procedure to isolate, from 3T3 cells, two variant cell lines, TNR-2 and TNR-9, which retain growth control and divide in response to elevated serum or fibroblast growth factor, but which do not respond to TPA. The variants do not incorporate precursors into DNA in response to TPA, demonstrating that the cells do not enter the S phase of the cell cycle. The TPA nonresponsive variant TNR-2 cannot respond to epidermal growth factor; TNR-9 responds to this mitogen. TNR-2 variant cells, which do not respond to EGF, do not bind 125I-EGF. TPA can modulate 125I-EGF binding to TNR-9 cells in a manner similar to its action on parental 3T3 cells. This TPA-induced alteration of EGF binding indicates that TNR-9 cells still interact with TPA, despite their inability to mount a mitogenic response.
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  • 193
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    Journal of Cellular Physiology 107 (1981), S. 85-100 
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: In an investigation of the changes that occur in cultured neoplastic cells as they outgrow their supply of nutrient, MM96 human melanoma cells were found to diminish in size and to proliferate more slowly. These changes were accompanied by a moderate increase in the proportion of cells with a G1-like DNA content. When replated under favorable conditions, many of these cells gradually resumed active proliferation. Continuing adverse culture conditions led to a continued fall in cell size, loss of reproductive viabllity, and finally to rapid cell death. Simultaneous buoyant-density and velocity-sedimentation-fractionation experiments showed that cells from exponential cultures were moderately dense and rapidly sedimenting, cells from postexponential cultures were less dense and much more slowly sedimenting, and dye-excluding cells from reproductively nonviable, late postexponential cultures were of widely variable though generally high density, and were moderately rapidly sedimenting. Although neither fractionation method resulted in significant enrichment of clonogenic cells, depletion was seen at both extremes of both types of profile. Cells fractionated by velocity were sorted according to DNA content and hence location in the cell cycle. The relationship between sedimentation rate and cell-cycle location was reflected in the continuous thymidine labeling patterns of the separated cells. Study of these patterns suggested that cycle durations lengthened as crowding increased and nutrient became depleted, and shortened upon reseeding at low density into fresh medium.
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  • 194
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    Journal of Cellular Physiology 107 (1981), S. 171-183 
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The production and localization of laminin, as a function of cell density (sparse versus confluent cultures) and growth stage (actively growing versus resting cultures), has been compared on the cell surfaces of cultured vascular and corneal endothelial cells. Comparison of the abilities of the two types of cells to secrete laminin and fibronectin into their incubation medium reveals that vascular endothelial cells can secrete 20-fold as much laminin as can corneal endothelial cells. In contrast, both cell types produce comparable amounts of fibronectin. Furthermore, if one compares the secretion of laminin and fibronectin as a function of cell growth, it appears that the laminin released into the medium by either vascular or corneal endothelial cells, is a function of cell density and cell growth, since this release is most pronounced when the cells are sparse and actively growing, and decreases by 10- and 30-fold, respectively, when either vascular or corneal endothelial cell cultures become confluent. With regard to fibronectin secretion, no such variation can be seen with vascular endothelial cell cultures, regardless of whether they are sparse and actively growing or confluent and resting. Corneal endothelial cell cultures, demonstrated a twofold increase in fibronectin production when they were confluent and resting as compared to when they were sparse and actively growing. When the distribution of laminin versus fibronectin within the apical and basal cell surfaces of cultured corneal and vascular endothelial cells is compared, one can observe that unlike fibronectin, which in sparse and subconfluent cultures can be seen to be associated with both the apical cell surface. In confluent cultures, laminin can be found associated primarily with the extracellular matrix beneath the cell monolayer, where it codistributes with type IV collagen.
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  • 195
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    Journal of Cellular Physiology 107 (1981), S. 209-217 
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Conditions have been established under which the antizyme of ornithine decarboxylase (E.C. 4.1.1.17, L-ornithine carboxy-lyase, ODC) a noncompetitive protein inhibitor of ODC, can be detected in cells in response to as little as 10-7 M putrescine. The maintenance of intracellular antizyme activity depends upon the continued presence of putrescine in the medium. Removal of putrescine results in a rapid decline of antizyme activity. These phenomena are unaffected by the presence of cycloheximide and are comparable to the requirement of L-asparagine for the maintenance of ODC activity.The extent to which the antizyme level is increased is inversely related to the preexisting level of intracellular ODC at the time of addition of putrescine. The time of appearance of free antizyme is delayed in cells that have high levels of ODC; the amount of free antizyme that can be assayed for in these cells, at any particular time is correspondingly less. The converse is also true. In cells that have high levels of antizyme, the delay in appearance of ODC is greater and the amount of ODC that can be assayed for is correspondingly less than in cells with low levels of antizyme.These experiments, as well as others, indicate that the ODC antizyme and ODC interact in vitro with each other to modify their respective activities.
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  • 196
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    Journal of Cellular Physiology 107 (1981), S. 243-249 
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Resting Yoshida AH130 hepatoma cells, harvested at the plateau of tumor development in vivo, were recruited into the cycling state following transfer to an in vitro system whereby these cells were incubated in the autologous ascites plasma diluted with buffered saline and enriched with glucose. In this system, cell recruitment into the phase of DNA synthesis (S phase) strictly depends on the activity of the respiratory chain and is abolished by anaerobiosis as well as by antimycin A, although the intracellular levels of ATP and the rate of protein synthesis are practically unaffected by these treatments. Furthermore, 2,4-dinitrophenol, at concentrations which uncouple the respiratory phosphorylation and hence enhance both glycolysis and oxygen consumption, does not hinder cell promotion into S phase. Thus, the absolute respiration dependence of cycling resumption by resting ascites cells does not seem to rely on respiratory ATP supply, but rather is linked to the electron flow through the respiratory chain.
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  • 197
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    Journal of Cellular Physiology 107 (1981), S. 271-281 
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: The synthesis and turnover of sulfate-labeled glycosaminoglycans (35S-GAGs) has been investigated in diploid human embryo fibroblasts during in vitro cellular aging. With progressive subcultivation, there was a decreased incorporation of Na235SO4 into 35S-GAGs released to the medium, but not into those accumulated at the cell surface. The composition of 35S-GAGs found in extracellular medium, cell surface (removable by gentle proteolysis), and intracellular compartments of the culture after 48-hr labeling did not change significantly with progressive subcultivation. Pulse-labeled 35S-GAGs moved from intracellular to surface and extracellular compartments more slowly in late-passage cultures. Addition of 1 mM β-xyloside to both early- and late-passage cultures produced a ten-fold enhancement of extracellular 35S-GAG production without a concomitant increase in surface-associated 35S-GAG. We interpret the data of this study to mean that secreted and cell-surface glycosaminoglycans represent different pools and that cellular aging has its effect primarily upon the secreted pool of glycosaminoglycans. Late-passage fibroblasts demonstrate marked decreases in proliferation, culture density, fibronectin matrix, and gap-junction formation. Our results suggest that glycosaminoglycan synthesis and composition are not intimately related to these parameters.
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  • 198
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    Journal of Cellular Physiology 107 (1981) 
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
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  • 199
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    Journal of Cellular Physiology 107 (1981), S. 317-327 
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: Conditioned serum-free medium (CSFM) obtained from WI-38 human fibroblasts was found to contain a mitogenic factor(s) with somatomedin (SM)-like activity. Treatment of the cells with cycloheximide eliminated the SM-like activity in CSFM, suggesting that these cells produce and release the activity. Gel filtration revealed that the fibroblast SM-like activity (FSLA) had a molecular size near 45,000. Isoelectric focusing of this FSLA yielded 2 bands of SM activity with pIs of 4.7 and 6.1, and corresponding molecular sizes of ∼29,000 and 16,500, respectively. The FSLA obtained by gel filtration revealed parallel dose response curves with a basic SM in a SM radioreceptor and radioimmunoassay and stimulated: (1) 35So4 uptake by hypophysectomized rat cartilage; (2) (U-14C) glucose oxidation is isolated rat adipocytes; and (3) (3H) thymidine uptake and cell division in these same WI-38 fibroblasts. Out studies indicate that this FSLA and basic SM are similar but not identical.
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  • 200
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    Journal of Cellular Physiology 107 (1981), S. 345-358 
    ISSN: 0021-9541
    Keywords: Life and Medical Sciences ; Cell & Developmental Biology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Medicine
    Notes: We have shown previously (D.A. Sirbasku, 1978, Proc. Natl. Acad. Sci. U.S.A., 75:3786-3790) that an estrogen-inducible growth factor activity for rat mammary and rat pituitary tumor cells can be identified in extracts of rat uteri, although at the time of that report only a limited biochemical characterization of the activity was presented. In this report, we have evaluated the growth factor activity for lipid, steroid hormone or protein-like properties. Uterine growth factor activity was assayed by measure of the increased cell number of the MTW9/PL rat mammary tumor cell line established by this laboratory and described previously (D.A. Sirbasku, 1978, Cancer Res. 38:1154-1165). Studies showed the following characteristics of growth factor activity: destroyed by trypsin treatment; labile when heated at 80°C; partially denatured by 6 M guanidine or 8 M urea treatment or 50% aqueous solutions of organic solvents; inactivated by extremes of pH or overnight treatment with mild acid; not dialyzable at neutral pH; of apparent molecular weight of 70,000 daltons by G-100 Sephadex chromatography; possessing an isoelectric point of 4.8 to 5.2; not chloroform/methanol extractable; and not in any way identified as either a lipid or a steroid hormone. The data available suggest that the uterine growth factor activity is a protein or polypeptide of apparent high molecular weight, and that this activity does not directly correspond to other known growth factors.
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