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  • Escherichia coli  (36)
  • Nitrogen fixation  (36)
  • Wheat  (33)
  • Springer  (105)
  • AGU (American Geophysical Union)
  • American Institute of Physics
  • Institut für Polarökologie Kiel
  • Lipsius & Tischer
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  • 2020-2022
  • 1985-1989  (105)
  • 1890-1899
  • 1987  (105)
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  • 2020-2022
  • 1985-1989  (105)
  • 1890-1899
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 4 (1987), S. 61-66 
    ISSN: 1432-0789
    Keywords: Stem nodulation ; Aeschynomene afraspera ; Legume ; Nitrogen fixation ; Acetylene reduction assay (ARA)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Aeschynomene afraspera is a wild annual legume growing in periodically waterlogged soils in western Africa. This legume is characterized by a profuse stem nodulation. Nodules are formed on the stem at the emergence of lateral root primordia, called nodulation sites. These sites are irregularly distributed on vertical rows all along the stem and branches. Stem nodules are hemispherically shaped. Their outside is dark green and they contain a red-pigmented central zone. Stem nodules exhibit a high nitrogen-fixing potential. Acetylene reduction assays result in stem nodule activity of 309 μmol C2H4 g−1 dry nodule h−1. Field-grown stem nodulated Aeschynomene accumulated more N (51 g N m−2 in 10 weeks) than the root nodulated one. Because of this nitrogenfixing potential and its ability to grow in waterlogged conditions, A. afraspera could probably be introduced into tropical rice cropping systems.
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 5 (1987), S. 83-87 
    ISSN: 1432-0789
    Keywords: Inoculation ; Inoculum dose ; Nitrogen fixation ; Chickpea ; Rhizobium spp. ; Cicer arietinum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The influence of three inoculum rates on the performance of three chickpea (Cicer arietinum L.) Rhizobium strains was examined in the field on a Mollisol soil. Increasing amounts of inoculum improved the performance of the strains. A normal dose (104 cells per seed) applied at different intervals gave non-significant increases in nodulation, nitrogenase activity (acetylene reduction assay), nitrogen uptake and grain yield. A ten-fold increase in inoculum increased nodule number, shoot dry weight, nitrogenase activity (ARA) and grain yield, but increases over the control were significant only for nodule dry weight and nitrogen uptake by shoot and grain. The highest level of inoculum (100 × normal) significantly increased nodule dry weight, grain yield, total nitrogenase activity (ARA) and nitrogen uptake by shoot and grain. Strain TAL 620 was more effective than the other two. Combined nitrogen (60 kg N ha−1) suppressed nodulation and nitrogenase activity (ARA).
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  • 3
    ISSN: 1432-0789
    Keywords: Triticum aestivum ; T. turgidum ; Nitrogen fixation ; Field inoculation ; Acetylene reduction assay (ARA)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Eight commercial Israeli spring wheat cultivars (six Triticum aestivum and two T. turgidum) grown with 40 and 120 kg N/ha were tested for responses to inoculation with Azospirillum brasilense. At the low level of N fertilization (40 kg/ha), five cultivars showed significant increases in plant dry weight measured at the milky ripe stage; however, by maturation only the cultivar “Miriam” showed a significant increase in grain yield. Two cultivars, which had shown a positive inoculation effect at the earlier stages, had a significant decrease in grain yield. No significant effect of inoculation was found at the high N level. To confirm those results, four wheat (T. aestivum) cultivars were tested separately over 4 years in 4 different locations under varying N levels. Only Miriam showed a consistently positive effect of Azospirillum inoculation on grain yield. Inoculation increased the number of roots per plant on Miriam compared with uninoculated plants. This effect was found at all N levels. Nutrient (N, P and K) accumulation and number of fertile tillers per unit area were also enhanced by Azospirillum, but these parameters were greatly affected by the level of applied N. It is suggested that the positive response of the spring wheat cultivar “Miriam” to Azospirillum inoculation is due to its capacity to escape water stresses at the end of the growth season.
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  • 4
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 4 (1987), S. 15-19 
    ISSN: 1432-0789
    Keywords: Nitrogen fixation ; N-balance studies ; Azolla ; Blue-green algae ; Chemical N fertilization ; Rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A nitrogen balance study conducted in ceramic pots under net house conditions for four seasons showed that flooded rice soil leaves a positive nitrogen balance (N increase) in soil after rice cropping in both fertilized and unfertilized soil. Recovery of nitrogen from rice soil was more than its input in unfertilized soil, but it was reverse in fertilized soil. Incorporation of Azolla or BGA twice as basal and 20 days after transplanting (DAT) alone or in combination showed higher nitrogen balance and N2-fixation (N gain) in soil than in that where it was applied once either as basal or 20 DAT. Planted soil showed more N2-fixation than that of fallow rice, and flooded soil fixed more nitrogen in comparison to non-flooded soil in light but less in dark. Soil exposed to light fixed more nitrogen than that of unexposed soil in both flooded and non-flooded conditions.
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  • 5
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 4 (1987), S. 9-14 
    ISSN: 1432-0789
    Keywords: Rhizosphere ; Nitrogen fixation ; Root exudates ; Soil bacteria ; Carbon budget ; Rice
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The association of rice seedlings (cv. Delta) with different strains of Azospirillum was studied under monoxenic conditions in the dark. Axenic 3-day-old seedlings were obtained on a C- and N-free medium and inoculated with 6 · 107 bacteria per plant in a closed vial. Seven days later, different components of a carbon budget were evaluated on them and on sterile controls: respired CO2, carbon of shoot and roots, bacterial and soluble carbon in the medium. Two strains (A. lipoferum 4B and A. brasilense A95) isolated from the rhizosphere of rice caused an increase in exudation, + 36% and + 17% respectively compared with sterile control. Shoot carbon incorporation and respiration were reduced by inoculation. A third strain (A. brasilense R07) caused no significant change in exudation. A. lipoferum B7C isolated from maize did not stimulate rice exudation either. We further investigated a possible effect of nitrogen fixation on this phenomenon: inhibition of nitrogen fixation by 10% C2H2 did not modify the extent of C exudation by rice associated with A. lipoferum 4B or with the non-motile A. lipoferum 4T.
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 4 (1987), S. 37-40 
    ISSN: 1432-0789
    Keywords: Azospirillum brasilense ; Azospirillum amazonense ; rate reductase ; Inoculation ; Wheat ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Three field experiments with wheat were conducted in 1983, 1984, and 1985 in Terra Roxa soil in Paraná, the major Brazilian wheat-growing region, to study inoculation effects of various strains of Azospirillum brasilense and A. amazonense. In all three experiments inoculation with A. brasilense Sp 245 isolated from surface-sterilized wheat roots in Paraná produced the highest plant dry weights and highest N% in plant tops and grain. Grain yield increases with this strain were up to 31 % but were not significant. The application of 60 or 100 kg N ha−1 to the controls increased N accumulation and produced yields less than inoculation with this strain. Another A. brasilense strain from surface-sterilized wheat roots (Sp 107st) also produced increased N assimilation at the lower N fertilizer level but reduced dry weights at the high N level, while strain Sp 7 + Cd reduced dry weights and N% in the straw at both N levels. The A. amazonense strain isolated from washed roots and a nitrate reductase negative mutant of strain Sp 245 were ineffective. Strains Sp 245 and Sp 107st showed the best establishment within roots while strain Cd established only in the soil.
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 4 (1987), S. 199-203 
    ISSN: 1432-0789
    Keywords: Wheat ; Barley ; Urea ; Sulfuric acid ; Straw decomposition
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Wheat straw treated with 0.5 or 1.0 ml/g urea-sulfuric acid (1:1 acid in water v/v) and incubated in Protneuf or Woodburn silt loam soils in the laboratory decomposed faster than nontreated straw the first 4–6 weeks but at 12 weeks the nontreated straw had decomposed 25%–45% more. In a field experiment, urea-sulfuric acid treated straw, removed at 40-day intervals over 160 days, decomposed faster than nontreated straw. The differences were attributed to salt buildup in the laboratory samples, where electrical conductivities up to 17.6 dS/m were observed. In the field, leaching removed the excess salts. Nitrification produced up to 1875 mg NO 3 − N/kg Portneuf silt loam soil in the laboratory, indicating that nitrifying bacteria were not suppressed by the salt. Total plate counts with no straw were 1.8 × 106 microorganisms/g and with urea-sulfuric acid treated straw were 15.7 × 106/g soil after 14 days incubation. The respective actinomycete counts were 0.3 × 106 and 6.7 × 106/g for the no straw and straw-treated soils, respectively. The urea-sulfuric acid treatments suppressed straw decomposition in the laboratory and accelerated straw decomposition in the field.
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Journal of molecular evolution 24 (1987), S. 205-211 
    ISSN: 1432-1432
    Keywords: Endosymbiont ; Aphid ; Genome size ; Nucleotide composition ; Cell organelle ; Mycoplasma ; Escherichia coli
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary An intracellular symbiont was isolated from the mycetocyte of the pea aphidAcyrthosiphon pisum, and its genomic DNA was compared with those ofEscherichia coli andMycoplasma capricolum with respect to nucleotide composition and kinetic complexity. Thermal dissociation, CsCl density equilibrium centrifugation, and high-performance liquid chromatography of the nuclease P1 digest all indicated that the G+C content of the endosymbiont DNA is as low as 30%. In this respect, the endosymbiont resembledMycoplasma species. The reassociation kinetics of genomic DNA labeled by nick translation suggested that the endosymbiont genome is 1.4×1010 daltons in size, about 5 and 18 times as large as those ofE. coli andM. capricolum, respectively. The results were confirmed by reassociation of endosymbiont DNA labeled by incubation with [3Hthymidine in Grace's medium. The endosymbiont genome of the aphid was about 500 times larger than those of leafhopper endosymbionts previously analyzed by ultracentrifugation. These characteristic properties of the aphid endosymbiont genome are discussed in connection with the evolution of cell organelles, and with reference to a previous finding that most of the genes of the aphid endosymbiont are not expressed when present intracellularly.
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  • 9
    ISSN: 1432-1939
    Keywords: Lupinus succulentus ; Fabaceae ; Lupine ; Quinolizidine alkaloids ; Nitrogen fixation ; Defoliation ; Plant-herbivore interactions
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We examined the effects of nitrogen nutrition and defoliation on the alkaloids, nitrogen levels, and growth of Lupinus succulentus by growing plants under five nitrogen/defoliation treatments: 1) fertilization with a high-nitrate nutrient solution, 2) fertilization with a low-nitrate solution, 3) inoculation with N-fixing bacteria but without available soil nitrogen, 4) high-nitrate solution plus periodic partial defoliation, and 5) low-nitrate plus defoliation. In the absence of defoliation, plants from both the N-fixing and high-N treatments had higher concentrations of alkaloids and nitrogen, and higher growth rates than the low-N plants. Periodic defoliation had little effect on the high-N plants, but defoliated N-fixing plants were severely stunted and had lower alkaloid and nitrogen levels. The experimental treatments also affected the relative concentrations of the alkaloids. Our results indicate that 1) alkaloid composition and concentration in L. succulentus are determined by both nitrogen availability and developmental state, 2) plants relying solely on N-fixation respond quite differently to defoliation than those with adequate soil nitrogen, and 3) the food value of the plant tissue can be affected by an interaction between the effects of defoliation and nitrogen status.
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  • 10
    ISSN: 1432-0983
    Keywords: Organellar genomes ; tRNA genes ; Wheat ; Maize
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We have used bean mitochondrial (mt) and chloroplast (cp) tRNATrp as probes to locate the corresponding genes on the mt and cp genomes of wheat and we have determined the nucleotide sequences of the wheat mt and cp tRNATrp genes and of the flanking regions. Sequence comparisons show that the wheat mt and cp tRNATrp genes are 97% homologous. On the wheat cp DNA, a tRNA Pro UGG gene was found 139 by upstream of the cp tRNATrp gene. On the wheat mt DNA, a sequence of 23 nucleotides completely homologous with the 3' end of this cp tRNAPro gene was found 136 by upstream of the rut tRNATrp gene, but there is only 38% homology between cp and mt wheat genomes in the intergenic regions. The overall organization of this region in the chloroplast genome (a tRNATrp gene separated by about 140 by from a tRNAPro gene) is also found in the mitochondrial genome, suggesting that this mitochondrial fragment might have originated from a chloroplast DNA insertion. A comparison of the genes and of the intergenic regions located between the tRNATrp gene and the tRNAPro (or partial tRNAPro) gene shows that there is an almost complete conservation of these sequences in the mitochondrial DNA of wheat and maize, whereas wheat mt and cp intergenic regions show more sequence divergence. Wheat mt tRNATrp gene is encoded by the main mt genome (accounted for by the master chromosome) but, in the case of maize mitochondria, this gene was found to be encoded by the 2.3 kb linear plasmid, indicating that this plasmid is not dispensable in maize mitochondria.
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