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  • Articles  (15)
  • 42.75
  • Animals
  • Chemical Engineering
  • gene transfer
  • Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition  (15)
  • 1
    Electronic Resource
    Electronic Resource
    An extended deletion map of the Lr19 translocation and modified forms (1998)
    Springer
    Euphytica 103 (1998), S. 95-102 
    Details
    ISSN: 1573-5060
    Keywords: gene transfer ; physical mapping ; RFLPs ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A physical deletion map of the Lr19 translocated chromosome segment was extended by mapping three additional Thinopyrum RFLP loci. The relative locations of the marker loci on the translocated segment were determined as: centromere, Sd1, Xpsr165, Xpsr105, Xpsr129, XcsIH81-1, Xwg380, Xmwg2062, Lr19, Wsp-D1, Sr25/Y. Various recombinants, putative recombinats and mutants of the Lr19 segment were also characterised with respect to the additional markers.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1018370718296
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  • 2
    Electronic Resource
    Electronic Resource
    Introduction of new traits into cotton through genetic engineering: insect resistance as example (1997)
    Springer
    Euphytica 96 (1997), S. 163-166 
    Details
    ISSN: 1573-5060
    Keywords: Agrobacterium tumefaciens ; Bacillus thuringiensis ; cotton ; gene transfer ; Gossypium hirsutum ; insect resistance ; protease inhibitors ; regeneration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The main goal of gene transfer into cotton is the development of insect-resistant varieties. The stakes are important since cotton protection against insects uses almost 24% of the world's chemical insecticides market, which is not without consequences on the environment. The first approach was to introduce and express in the cotton genome, genes from the bacterium Bacillus thuringiensis (B.t.) which produces entomopathogenic toxins. The development of an efficient Agrobacterium tumefaciens mediated transformation system was the first step. The expression of B.t. genes was studied and synthetic genes more adapted to a plant genome have been constructed. Studies on their expression in cotton is underway. The second focus was to develop strategies that would minimize the risks of inducing insect resistance. The main approach is to associate several genes coding for entomopathogenic proteins with different modes of action. Genes encoding protease inhibitors were chosen. One possibility is to associate a B.t. gene and a gene encoding a protease inhibitor. Several protease inhibitors were tested in artificial diets on major pests of cotton. The corresponding genes have been introduced into the cotton genome. These various orientations of the research program will be presented.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1002906308304
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  • 3
    Electronic Resource
    Electronic Resource
    Animal Consciousness and Ethics in Asia and the Pacific (1997)
    Springer
    Journal of agricultural and environmental ethics 10 (1997), S. 249-267 
    Details
    ISSN: 1573-322X
    Keywords: Animals ; Asia ; consciousness ; Australia ; Hong Kong ; India ; Israel ; Japan ; New Zealand ; The Philippines ; Russia ; Singapore ; Thailand
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition , Philosophy
    Notes: Abstract The interactions between humans, animals and the environment have shaped human values and ethics, not only the genes that we are made of. The animal rights movement challenges human beings to reconsider interactions between humans and other animals, and maybe connected to the environmental movement that begs us to recognize the fact that there are symbiotic relationships between humans and all other organisms. The first part of this paper looks at types of bioethics, the implications of autonomy and the value of being alive. Then the level of consciousness of these relationships are explored in survey results from Asia and the Pacific, especially in the 1993 International Bioethics Survey conducted in Australia, Hong Kong, India, Israel, Japan, New Zealand, The Philippines, Russia, Singapore and Thailand. Very few mentioned animal consciousness in the survey, but there were more biocentric comments in Australia and Japan; and more comments with the idea of harmony including humans in Thailand. Comparisons between questions and surveys will also be made, in an attempt to describe what people imagine animal consciousness to be, and whether this relates to human ethics of the relationships.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007774409131
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  • 4
    Electronic Resource
    Electronic Resource
    Potential for wild species in cool season food legume breeding (1993)
    Springer
    Euphytica 73 (1993), S. 109-114 
    Details
    ISSN: 1573-5060
    Keywords: wild-germplasm ; interspecific-hybridization ; gene pools ; introgression ; gene transfer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Wild species which are crossable to cultivated pea, lentil, and chickpea have been collected and are maintained in major germplasm collections throughout the world. Wild species of Vicia crossable to the cultivated faba bean have not been found. The primary, secondary, and tertiary gene pools of the cool season food legumes represent potential genetic diversity that may eventually be exploited in cultivated types to overcome biotic and abiotic stresses. Technical difficulties in obtaining hybrids beyond those within the primary gene pool is a major obstacle. Reproductive isolation, embryo breakdown, hybrid sterility, and limited genetic recombination are major barriers to greater use of wild germplasm. Conventional crossing has been successful in producing interspecific hybrids in Lens, Cicer and Pisum and those hybrids are being evaluated for desired recombinants. In vitro culture of hybrid embryos has been successful in overcoming barriers to wider crosses in Lens. The successful transfer of genes from wide sources to cultivated types can be assisted by repeated backcrossing and selection designed to leave behind undesired traits while transferring genes of interest. Molecular marker assisted selection may become a valuable tool in the future use of wild species. In general, too little is known about the possible genetic variation available in wild species that could be valuable in developing resistance to biotic and abiotic stresses. Current efforts on the use of wide hybridization in the cool season food legumes are reviewed and discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00027187
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  • 5
    Electronic Resource
    Electronic Resource
    The potential of gene technology and genome analysis for cool season food legume crops: theory and practice (1993)
    Springer
    Euphytica 73 (1993), S. 177-189 
    Details
    ISSN: 1573-5060
    Keywords: gene cloning ; gene transfer ; virus and insect resistance ; genome analysis ; DNA figerprinting
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The potential of plant gene technology encompasses a multitude of different techniques ranging from the isolation of useful genes, their characterization and in vitro manipulation to the reintroduction of the modified constructs into target plants, where they are expressed at a rate that alters the phenotype of the plants. Genome analysis, on the other hand, aims at characterizing the genome architecture and function(s). Plant gene technology has catalyzed progress in plant breeding, as will be exemplified by a few examples, but has not yet been applied to food legume improvement on a large scale. Genome analysis, however, has a series of practical implications, as is illustrated by the successful introduction of DNA fingerprint and PCR fingerprint techniques to chickpea (Cicer arietinum L.) breeding and Ascochyta rabiei pathotyping. The present overview addresses both areas of plant molecular biology to illustrate their potential for food legume breeding.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00027193
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  • 6
    Electronic Resource
    Electronic Resource
    Genetic improvement of legumes using somatic cell and molecular techniques (1991)
    Springer
    Euphytica 55 (1991), S. 157-169 
    Details
    ISSN: 1573-5060
    Keywords: gene transfer ; genetic manipulation ; chimaeric genes ; legumes ; transformation ; somatic hybridisation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The merits and limitations of somatic cell techniques involving Agrobacterium-mediated transformation, direct gene transfer and protoplast fusion, are discussed in relation to the genetic improvement of forage and grain legumes. Whilst progress with legumes is limited compared to that with plants of other families such as the Solanaceae, the fact that many legumes are readily amenable to tissue culture now permits somatic cell techniques to be targetted to these species. Future development of the subject will necessitate close collaboration between molecular biologists and plant breeders to enable novel plants generated by in vitro technologies to be incorporated into conventional breeding programmes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00025229
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  • 7
    Electronic Resource
    Electronic Resource
    Interspecific transfer of Brassica juncea-type high blackleg resistance to Brassica napus (1984)
    Springer
    Euphytica 33 (1984), S. 295-303 
    Details
    ISSN: 1573-5060
    Keywords: Brassica napus ; rapeseed ; Brassica juncea ; Leptosphaeria maculans ; blackleg resistance ; interspecific cross ; gene transfer ; polygenic resistance ; seedling and adult resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Complete resistance to Leptosphaeria maculans, the cause of blackleg of oilseed rape (Brassica napus), was transferred from B. juncea to B. napus through an interspecific cross. B. juncea-type complete resistance (JR) was recognized first in one F3 progeny (OnapJR) by the absence of leaf-lesions on seedlings and canker-free adult plants. The commercially important characters of B. napus were retained in advanced lines of OnapJR, which combined JR with low erucic acid levels (〈0.5%), high seed yield and variable maturity dates. JR appeared to be inherited as a major gene or genes. Segregation for resistance and susceptibility contintied to occur during later generations of selection of OnapJR. JR was readily transferred from OnapJR to other suitable B. napus cultivars or lines with partial resistance to blackleg and resulted in highly vigorous carly generation selections adapted to cold, wet situations along with complete resistance to blackleg.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00021125
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  • 8
    Electronic Resource
    Electronic Resource
    Gene transfer from non-tuberous to tuberous Solanum species: Evidence from meiosis in hybrids (1982)
    Springer
    Euphytica 31 (1982), S. 565-572 
    Details
    ISSN: 1573-5060
    Keywords: Solanum ; potato ; gene transfer ; interspecific hybridization ; meiosis ; chromosome doubling ; non-tuberous
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The distant hybrids between non-tuberous Solanum species and tuberous S. pinnatisectum display little or no pairing in F1 and predominantly bivalent formation (preferential pairing) after chromosome doubling. In such a situation the question about the potential and extent of gene transfer from the non-tuberous parent to the tuberous one is relevant to potato breeding. This question was investigated by studying meiosis in triploid and hexaploid hybrids from crosses between diploid TV5 x tetraploid (S. etuberosum x S. pinnatisectum). TV5 is similar to S. verrucosum with cytoplasm of S. tuberosum. The following evidence was found for the desirable transfer of S. etuberosum genes to the tuberous species. The triploid F1 hybrids did not display the configurations 12 II+12 I expected if no gene exchange would take place between S. etuberosum and the tuberous species; however, a considerable number of multivalents per cell was observed in all plants studied. In the hexaploid F1 hybrids, obtained from the triploids through somatic doubling in vitro, 36 bivalents could reasonably be expected. Although bivalents were predominant (an overall average of 24.2 per cell) quite a few chromosomes were associated as multivalents in all plants investigated. It is concluded that in the hybrids studied a considerable amount of pairing and chiasma formation occurs between chromosomes of non-tuberous and those of tuberous Solanum species. This pairing affinity is larger than that found in 2x and 4x hybrids from S. etuberosum x S. pinnatisectum. Some hypotheses are put forward to explain this increased pairing affinity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00039194
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  • 9
    Electronic Resource
    Electronic Resource
    The structure and breeding behaviour of a translocation involving the transfer of mildew resistance from Avena barbata Pott. into the cultivated oat (1978)
    Springer
    Euphytica 27 (1978), S. 731-739 
    Details
    ISSN: 1573-5060
    Keywords: Avena sativa ; Avena barbata ; oats ; mildew resistance ; gene transfer ; alien variation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The gene for mildew resistance has been succesfully transferred into the cultivated oat from the wild oat species Avena barbata by means of an irradiation-induced translocation. The translocation has been shown to involve the long arm of chromosome ST21 of A. sativa, the short arm, the centromere and a segment of the long arm of the barbata chromosome. The transmission of the translocation is normal in the cultivar Manod in which it was originally isolated. When the translocation was transferred into other cultivars of oats, transmission through the male gametes was found to be impaired in some genotypic backgrounds. However, there was no evidence that the translocation had any deleterious effect on development and fertility in a range of cultivars. The translocation was shown to involve an exchange between nonhomoeologous chromosomes. The behaviour of the translocation in diverse genotypes indicated that the translocation was a new source of mildew resistance that could be easily used in a breeding programme.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023709
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  • 10
    Electronic Resource
    Electronic Resource
    Domestication of the protein-rich tetraploid wild oats Avena magna and A. Murphyi (1977)
    Springer
    Euphytica 26 (1977), S. 221-223 
    Details
    ISSN: 1573-5060
    Keywords: wena magna ; Avena murphyi ; cultivated oats ; wild oats ; interspecific hybrid ; gene transfer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The first stage in the domestication of the protein-rich tetraploid oats Avena magna and A. murphyi has been achieved by introducing seed non-shattering from the cultivated hexaploid oats A. sativa. A great variation in the protein content was encountered in the tetraploid domesticated types and plants having up to 27 31% protein were selected. The potential of these newly domesticated oats in increasing protein yields is briefly diseussed: also the significance of the tetraploids A magna and A. murphyi in improving the protein content of the hexaploid cultivars is stressed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00032088
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  • 11
    Electronic Resource
    Electronic Resource
    Shoot apical meristems as a target for gene transfer by microballistics (1955)
    Springer
    Euphytica 85 (1955), S. 45-51 
    Details
    ISSN: 1573-5060
    Keywords: meristem ; shoot apex ; ballistic microtargeting ; gene transfer ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The classical approach of gene transfer to a given plant species delivers the foreign gene to transformable cells and then puts the effort into generating plants. This approach is very difficult in many important crop plants, including cereals, and the results of regeneration are very genotype-dependent. In contrast, we use regenerable cells and try to transform them. Shoot apical meristems provide a tissue which regenerates in situ a fertile plant for most given genotypes or species. Transformation of meristem cells may lead to transgenic sectors in chimeras. These sectors may contribute to the gametes and, thus, to transgenic offspring, which then should be homohistonts and not sectorial chimeras like their parents. Our model plant for these studies is wheat. Microtargeting is a ballistic approach which is particularly suitable for the controlled delivery of microprojectiles to meristem cells in situ (Sautter et al., 1991). We summarize in this paper our experience with ballistic microtargeting of transgenes to wheat shoot apical meristem cells in situ.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023929
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  • 12
    Electronic Resource
    Electronic Resource
    Transgenic barley by particle bombardment. Inheritance of the transferred gene and characteristics of transgenic barley plants (1955)
    Springer
    Euphytica 85 (1955), S. 81-88 
    Details
    ISSN: 1573-5060
    Keywords: gene transfer ; Hordeum vulgare ; neomycin phosphotransferase II ; particle bombardment ; transgenic barley
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Transgenic barley plants (Hordeum vulgare L. cv. Kymppi) were obtained by particle bombardment of various tissues. Immature embryos and microspore-derived cultures were bombarded with gold particles coated with plasmid DNA carrying the gene coding for neomycin phosphotransferase II (NPTII), together with plasmid DNA containing the gene for β-glucuronidase (GUS). Bombarded immature embryos were grown to plants without selection and NPTII activity was screened in small plantlets. One plant proved to be transgenic (T0). This chimeric plant passed the transferred nptII gene to its T1 progeny. The presence of the nptII gene was demonstrated by the PCR technique and enzyme activity was analyzed by an NPTII gel assay. Four T0 spikes and 15 T1 offspring were transgenic. The integration and inheritance was confirmed by Southern blot hybridization. Transgenic T2 and T3 plants were produced by isolating embryos from green grains of transgenic T1 and T2 plants, respectively and growing them to plants. After selfing, the ratio of transgenic to non-transgenic T2 offspring was shown to follow the rule of Mendelian inheritance. The general performance of transgenic plants was normal and no reduction in fertility was observed. Microspore-derived cultures were bombarded one and four weeks after microspore isolation. After bombardment, cultures were grown either with or without antibiotic selection (geneticin R or kanamycin). When cultures were grown without selection and regenerated plants were transferred to kanamycin selection in rooting phase, one out of a total of about 1500 plants survived. This plant both carried and expressed the transferred nptII gene. The integration was confirmed by Southern blot hybridization. This plant was not fertile.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023933
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  • 13
    Electronic Resource
    Electronic Resource
    The sulphur-rich Brazil nut 2S albumin is specifically formed in transgenic seeds of the grain legume Vicia narbonensis (1955)
    Springer
    Euphytica 85 (1955), S. 181-192 
    Details
    ISSN: 1573-5060
    Keywords: Vicia narbonensis ; gene transfer ; gene expression ; seeds ; 2S albumin ; methionine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Epicotyl explants were co-cultivated with Agrobacterium tumefaciens EHA101 to transfer a chimeric 2S albumin gene construct carried in the binary Ti plasmid vectors pGSGLUC1 or pGA472 into the grain legume Vicia narbonensis. This gene encoding the sulphur-rich Brazil nut albumin was under the control of either the CaMV 35S promoter which permits gene expression in all organs, or the Vicia faba legumin B4 promoter which elicits seed-specific gene expression. After callus formation and selection for kanamycin resistance, somatic embryos were induced which, in the case of transformation with the vector pGSGLUC1, were screened for GUS activity. Embryos that produced GUS were in addition analysed for 2S albumin formation. Selected transgenic embryos were cloned by multiple shoot regeneration. Rooted and fertile plants were obtained by grafting transgenic shoots on the appropriate seedlings. R1 and R2 generations were raised and analysed for GUS as well as 2S albumin gene expression. Expression of the 35S promoter/2S albumin gene fusion took place in all organs of the transgenic plants including the cotyledons of seeds, whereas seed-specific gene expression was found in transformants with the legumin promoter/2S albumin gene fusion. The 2S albumin accumulated in the 2S protein fraction of transgenic seeds and its primary translation product was processed into the 9 and 3 kDa polypeptide chains. The foreign protein was localised in the protein bodies of the grain legume. Analysis of the R2 plants indicated Mendelian inheritance of the 2S albumin gene. In homozygous V. narbonensis plants the amounts of 2S albumin were twice that present in the corresponding heterozygous plants. Whereas only low level formation of the foreign protein was achieved if the gene was under the control of the 35S promoter, approximately 3.0% of the soluble seed protein was 2S albumin if seed-specific gene expression was directed by the legumin B4 promoter. Some of these transformants exhibited a three-fold increase in the methionine content of the salt-soluble protein fraction extracted from seeds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023947
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  • 14
    Electronic Resource
    Electronic Resource
    Strategies for variety-independent genetic transformation of important cereals, legumes and woody species utilizing particle bombardment (1955)
    Springer
    Euphytica 85 (1955), S. 13-27 
    Details
    ISSN: 1573-5060
    Keywords: gene transfer ; crop species ; particle bombardment ; transgenic plants ; cereals ; legumes ; woody plants
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The limiting component in the creation of transgenic crops has been the lack of effective means to introduce foreign genes into elite germplasm. However, the development of novel direct DNA transfer methodology, by-passing limitations imposed by Agrobacterium-host specificity and cell culture constraints, has allowed the engineering of almost all major crops, including formerly recalcitrant cereals, legumes and woody species. The creation of transgenic rice, wheat, maize, barley, oat, soybean, phaseolus, peanut, poplar, spruce, cotton and others, in an efficient and in some cases, variety-independent fashion, is a significant step towards the routine application of recombinant DNA methodology to the improvement of most important agronomic crops. In this review we will focus on key elements and advantages of particle bombardment technology in order to evaluate its impact on the accelerated commercialization of products based on agricultural biotechnology and its utility in studying basic plant developmental processes and function through transgenesis. Fundamental differences between conventional gene transfer methods, utilizing Agrobacterium vectors or protoplast/suspension cultures, and particle bombardment will be discussed in depth.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023926
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  • 15
    Electronic Resource
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    Improvement of milk protein quality by gene technology
    Amsterdam : Elsevier
    Livestock Production Science 35 (1993), S. 95-115 
    Details
    ISSN: 0301-6226
    Keywords: Dairy ruminants ; gene transfer ; milk protein genes ; polymorphism
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0301-6226(93)90184-J
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