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  • Chromatographie, Dünnschicht  (90)
  • Phytochrome  (56)
  • Springer  (146)
  • American Institute of Physics (AIP)
  • American Meteorological Society (AMS)
  • Nature Publishing Group
  • 1975-1979  (146)
Collection
Keywords
Publisher
  • Springer  (146)
  • American Institute of Physics (AIP)
  • American Meteorological Society (AMS)
  • Nature Publishing Group
Years
Year
  • 1
    Electronic Resource
    Electronic Resource
    Effect of light on the development of glyoxysomal functions in the cotyledons of mustard (Sinapis alba L.) seedlings (1979)
    Springer
    Planta 145 (1979), S. 181-186 
    Details
    ISSN: 1432-2048
    Keywords: Citrate synthase ; Fat degradation ; Isocitrate lyase ; Malate synthase ; Phytochrome ; Sinapis ; Starch (transitory)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The degradation of storage fat in the cotyledons of mustard seedlings is unaffected by phytochrome and photosynthesis (irradiation with continuous red or far-red light from sowing of the seeds) although light imposes a strong constraint on the translocation of organic matter from the cotyledons into the seedling axis. Likewise, the development and disappearance of glyoxysomal enzyme activities (isocitrate lyase, malate synthase, citrate synthase) takes place independently of light. It is concluded that the mobilization of storage fat (fat→carbohydrate transformation) is independent of photomorphogenesis. The surplus of carbohydrate produced from fat in the light seems to be converted to starch grains in the plastids, which function as a secondary storage pool in the cotyledons.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388715
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  • 2
    Electronic Resource
    Electronic Resource
    Phytochrome-mediated uptake of calcium in Mougeotia cells (1979)
    Springer
    Planta 146 (1979), S. 31-39 
    Details
    ISSN: 1432-2048
    Keywords: Autoradiography ; Calcium ; Mougeotia ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Ca2+ is proposed to function as a messenger in such phytochrome-mediated responses as localized cell growth, intracellular movements, and control of plasma membrane properties. To test this hypothesis, the uptake of Ca2+ in irradiated and non-irradiated regions of individual threads of the green alga Mougeotia was studied with the aid of 45Ca2+ and low temperature autoradiography: 10–20 cells within 40–60 cell-long threads were irradiated for up to 1 min, transferred to darkness for 3 to 10 min, submersed in a radioactive medium for 1 min, washed in an unlabelled medium for 30 min, and then autoradiographed at-80° C for several days. The autoradiographs show that those cells which had been pre-irradiated with red light did take up 2–10 times more Ca2+ than the adjacent non-irradiated cells of the same thread. Cells pre-irradiated with farred light or red light followed by far-red light showed no enhanced uptake of Ca2+. These results might be interpreted to indicate, firstly, that phytochrome-Pfr is involved in the enhanced uptake of Ca2+ and secondly, that the accumulation of radioactive Ca2+ in red light irradiated cells is an expression of an increased intracellular concentration of Ca2+. This interpretation is based on the data that (i) the dark interval between irradiation and labelling precluded the involvement of photosynthesis, (ii) the effect of red light was reversible with far-red light, and (iii) the accumulation of Ca2+ persisted during the long wash-out period. We speculate, that the red light-enhanced accumulation of Ca2+ in Mougeotia cells is caused by a Pfr-mediated increase of the Ca-permeability of the plasma membrane, and perhaps by a Pfr-impeding of an active Ca2+-extrusion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00381252
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  • 3
    Electronic Resource
    Electronic Resource
    Control of mitochondrial activities by phytochrome during greening (1979)
    Springer
    Planta 147 (1979), S. 229-235 
    Details
    ISSN: 1432-2048
    Keywords: Avena ; Greening ; Mitochondria ; Oxidative Phosphorylation ; Phytochrome ; Respiration
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Mitochondria isolated from 7-day old darkgrown Avena sativa L. (var. Arnold) laminae given 5 min illumination of red light, followed by varying lengths of darkness up to 3 h, showed at least a twofold increase in the rates of both NADH-dependent oxygen consumption and respiratory chain phosphorylation over those of mitochondria isolated from unilluminated tissue. Similar organelles, isolated from tissue given either far-red or red followed by far-red pretreatment, exhibited rates of both functions of between 25% and 75% below those of the mitochondria from unilluminated tissue. The induction-reversion criteria for phytochrome control of respiration and oxidative phosphorylation were satisfied under all experimental conditions during the greening process. Treatment with continuous far-red light, acting presumably through the ‘high irradiance’ reaction of phytochrome, served to disengage phytochrome activity from photosynthesis. The stimulation of oxidative phosphorylation still occurred under these conditions, slightly slower but much more prolonged in the absence of ATP from photophosphorylation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388743
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  • 4
    Electronic Resource
    Electronic Resource
    Phytochrome and internode elongation in Chenopodium polyspermum L. sites of photoreception (1979)
    Springer
    Planta 145 (1979), S. 405-409 
    Details
    ISSN: 1432-2048
    Keywords: Chenopodium ; Internode elongation ; Photoreception sites ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The elongation of the fourth internode of ‘fully green’ Chenopodium polyspermum L. is modulated by far-red light (FR) given in addition to the main light period. Two different types of organs are responsible for the photoreception of FR producing the end-of-day effect; the stem and the leaves situated just above and below the reacting internode. Photoreversibility can be obtained within certain limits in the two organs. Evidence is presented which shows that in the ‘fully green’ plant there is an interorgan reaction whose primary reaction is the photoconversion of phytochrome.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00380093
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  • 5
    Electronic Resource
    Electronic Resource
    Inhibition of carotenoid biosynthesis by the herbicide SAN 9789 and its consequences for the action of phytochrome on plastogenesis (1979)
    Springer
    Planta 145 (1979), S. 497-505 
    Details
    ISSN: 1432-2048
    Keywords: Carotenoids ; Chlorophylls ; Herbicides ; Phytochrome ; Plastid development ; Ribulosebisphosphate carboxylase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Treatment of the mustard (Sinapis alba L.) seedling with the herbicide SAN 9789 inhibits synthesis of colored carotenoids and interferes with the formation of plastid membrane lipids without affecting growth and morphogenesis significantly. In farred light, which is hardly absorbed by chlorophyll, development of plastid ultrastructure, synthesis of ribulosebisphosphate carboxylase and synthesis of chlorophyll are not affected by SAN 9789. It is concluded that normal phytochrome actions on plastid structural development, protein and chlorophyll syntheses are not affected by the absence of carotenoids provided that there is no significant light absorption in chlorophyll. The findings show that the inhibition of synthesis of one set of plastid membrane components (the carotenoids) does not stop synthesis of other components such as chlorophyll and does not halt membrane assembly. Supplementary experiments with the closely related compound SAN 9785, which affects the amount and composition of plastid lipids but not carotenoid and chlorophyll syntheses, suggest that the effect of the herbicide SAN 9789 is due exclusively to its inhibition of synthesis of colored carotenoids. In the presence of SAN 9789 white or red light at high fluence rate causes photodestruction of chlorophyll and ribulosebisphosphate carboxylase and photodecomposition of thylakoids. These effects are interpreted as resulting exclusively from the self-photooxidation and photosensitizing action of chlorophyll once the protection by carotenoids of chlorophyll against self- and sensitized photooxidation is lost.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00380105
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  • 6
    Electronic Resource
    Electronic Resource
    A systematic relationship between phytochrome-controlled development and species habitat, for plants grown in simulated natural radiation (1979)
    Springer
    Planta 145 (1979), S. 253-258 
    Details
    ISSN: 1432-2048
    Keywords: Phytochrome ; Development habitat ; Natural radiation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A survey of the responsiveness of plant species, typical of open and shade habitats, to simulated natural shade-light quality (i.e. white light plus supplementary far-red) has demonstrated a systematic relationship between habitat and certain developmental responses. Supplementary far-red light has a much greater effect on stem extension rate, petiole length, and leaf dry weight: stem dry weight ratio of the open habitat, shade-intolerant species. Far-red effects on leaf chlorophyll content show no such systematic grading. These results are discussed in relation to habitat adaptation. In most cases, the relationship between developmental response and the estimated phytochrome photoequilibrium, which is established by the radiation treatment, is linear. This is taken as an indication of phytochrome involvement in shade perception.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00454449
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  • 7
    Electronic Resource
    Electronic Resource
    Floral initiation in sorghum hastened by gibberellic acid and far-red light (1979)
    Springer
    Planta 145 (1979), S. 269-272 
    Details
    ISSN: 1432-2048
    Keywords: Flowering ; Gibberellin ; Photoperiodism ; Phytochrome ; Sorghum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Combinations of far-red light (FR) (4 min) and gibberellic acid (GA3), given at the beginning of a daily 12-h dark period in a growth room, were used to study floral induction in four maturity genotypes of the milo group of sorghum (Sorghum bicolor (L.) Moench). The 12-h dark period without GA3 application or FR induced flowering in only the early genotype; FR hastened initiation in the early genotype, while GA3 hastened floral initiation in the two intermidiate-flowering genotypes. GA3 and FR together had a strong synergistic effect, hastening floral initiation by 30 to more than 80 d in the early and intermediate genotypes. Red light (R) did not hasten flowering; FR preceded by R gave the same effect as FR alone. GA3 promoted stem elongation equally whether floral initiation occurred or not; thus, its effect on stem elongation was independent of floral initiation. The capacity of GA3 to induce flowering in sorghum, a short-day plant, seems to be enhanced by phytochrome being in the PR form at the beginning of the night when GA3 was applied.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00454451
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  • 8
    Electronic Resource
    Electronic Resource
    Action spectra for light-induced germination in dormant lettuce seeds (1979)
    Springer
    Planta 144 (1979), S. 133-136 
    Details
    ISSN: 1432-2048
    Keywords: Action spectrum ; Dormancy (seeds) ; Germination (seeds) ; Lactuca ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Light of wavelengths in the range 400–500 nm induces germination of seeds of Lactuca sativa L. (cv. Noran and May Queen) when made light requiring by high temperature (thermodormancy) or prolonged exposure to far red light (far red dormancy). TD seeds proved more sensitive to blue light than FRD seeds by several orders of magnitude. Blue induced germination could be reversed by far red. Fluence response curves for this reversion are less steep for TD than for FRD seeds. The degree of germination of TD seeds after moderate doses of blue light is decreased by dark storage at 22° C or by exposure to far red and this is accompanied by a marked change in the shape of the fluence response curves. In these respects, germination induction by blue resembles that by red light. Continuous exposure to blue light inhibits germination of TD seeds but hardly that of FRD seeds. An action spectrum is given for blue induced germination of TD seeds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00387261
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  • 9
    Electronic Resource
    Electronic Resource
    Action spectra for light-induced germination in dormant lettuce seeds (1979)
    Springer
    Planta 144 (1979), S. 125-131 
    Details
    ISSN: 1432-2048
    Keywords: Action spectra ; Dormancy (seeds) ; Germination (seeds) ; Lactuca ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Fluence response curves for red light-induced germination of thermodormant (TD) seeds of Lactuca sativa L. show two regions that differ in their light sensitivity. In the region of high sensitivity, the germination responses differ between seed batches and can be altered by dark storage or far red irradiation. Induction of germination in far red dormant (FRD) seeds requires far higher fluences. Action spectra for induction to 60% germination were determined for these various response types. Spectra for the regions of low sensitivity response are similar for TD and FRD seeds. In comparison, the action spectrum for the highly sensitive response in TD seeds is significantly shifted to longer wavelengths. Analogous differences exist in the action spectra for far red reversal of the red induced germination responses. Germination induction in the low sensitivity region shows repeated red-far red reversibility. Far red reversal of red induction in the high sensitivity region does not saturate even at the highest far red fluences available and requires increased red fluences for subsequent reinduction. A model quantitatively accounting for these observations is presented. It is pointed out that action spectra of processes involving photoreversible pigments with partly overlapping absorption spectra in general are not identical with the absorption spectra of the partners. They should depend upon the degree of phototransformation required to elicit a given physiological response. In the case of induction of lettuce seed germination the observed action spectra can be interpreted as reflecting different requirements for P fr of the various response types. Our results do not necessitate the assumption of spectroscopically different forms of phytochrome in these seeds.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00387260
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  • 10
    Electronic Resource
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    Red/far-red modulation in vitro of enzyme activity in a membrane fraction from Phaseolus aureus (1979)
    Springer
    Planta 146 (1979), S. 75-81 
    Details
    ISSN: 1432-2048
    Keywords: Adenosine-5′-triphosphate ; Membrane ; Phaseolus ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In a membrane fraction isolated from hypocotyls of Phaseolus aureus Roxb. the activity of a number of enzymes was regulated by red and far-red irradiation in vitro, provided that the tissue received a brief red light treatment before extraction. Other enzymes showed no photoregulation. There were two types of photocontrol, neither of which could be detected in the solute fraction, nor in extracts from completely etiolated material. One (Type I) was a red/far-red reversible regulation of the rate of enzyme activity, depending on the light given (in vivo or in vitro) before the assay was begun. The second (Type II) was a promotion of enzyme activity by red or far-red light given during the assay. The action spectra for type II responses do not coincide with either the phytochrome absorption or difference spectra. However, the effectiveness of red and far-red was correlated with the Pfr/P ratio present at the beginning of the assay, such that far-red was more efficient at high Pfr/P and red at low Pfr/P ratios. All enzymes that were regulated involved ATP. In samples that showed enzyme regulation, small changes in fluorescence yield of tryptophan and the covalent probe “Fluram” (Roche) accompanied the photoconversion of phytochrome, but no fluorescence changes could be measured after briefly incubating the membrane fraction with ATP. The results indicate that light may affect the interaction of ATP with the membrane fraction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00381258
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  • 11
    Electronic Resource
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    Far-red mediated polyribosome formation in radish cotyledons (1979)
    Springer
    Planta 147 (1979), S. 1-5 
    Details
    ISSN: 1432-2048
    Keywords: Polyribosomes ; Phytochrome ; Raphanus ; Ribonucleases
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The total ribosome content of radish cotyledons increases during the first 2–3 days of germination both in darkness and under far-red light irradiation; ribonuclease activity is not under phytochrome control during this period. Changes in ribonuclease activity interfere with the analysis of the polyribosomal population. A maximal ratio of polysomes to monosomes is observed 12 h after the onset of far-red light and then it decreases. A 12 h far-red irradiation stimulates the in vivo incorporation of amino acids into proteins. This stimulation persists when seedlings are transferred for 4 h to the dark.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384582
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  • 12
    Electronic Resource
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    Phytochrome-regulated transfer of fructosidase from cytoplasm to cell wall in Raphanus sativus L. hypocotyls (1979)
    Springer
    Planta 147 (1979), S. 7-13 
    Details
    ISSN: 1432-2048
    Keywords: Cell wall ; Cytoplasm ; β-Fructosidase (transfer) ; Phytochrome ; Raphanus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The far-red absorbing form of phytochrome, Pfr, rapidly increases the rate of transfer of β-fructosidase (E.C.3.2.1.26) from the cytoplasm to the cell wall in radish hypocotyls. Far-red light increases the level of enzyme in a particulate fraction: after two hours of light treatment, the particulate enzyme is associated almost exclusively with the endoplasmic reticulum. Transfer from the endoplasmic reticulum to the cell wall involves an incorporation into Golgi bodies and the plasmalemma: these membrane fractions were separated by centrifugation on a discontinuous sucrose density gradient and their degree of purity was determined by the use of known biochemical markers. With respect to β-fructosidase, light controls, via Pfr: (1) the total amount, (2) the incorporation into the endoplasmic reticulum and (3) the transfer to the cell-wall. These three processes have different sensitivities to cycloheximide.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384583
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  • 13
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    The effect of light pretreatments on phytochrome-mediated induction of anthocyanin and of phenylalanine ammonia-lyase (1979)
    Springer
    Planta 146 (1979), S. 369-376 
    Details
    ISSN: 1432-2048
    Keywords: Anthocyanin ; Mode of action of phytochrome ; Phenylalanine ammonia-lyase ; Phytochrome ; Sinapis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Induction by light of phenylalanine ammonia-lyase (PAL; EC 4.3.1.5) and of anthocyanin in cotyledons of the mustard (Sinapis alba L.) seedling is strongly affected by a light pretreatment which operates through phytochrome. If PAL or anthocyanin is induced by a light pulse, the effectiveness of phytochrome (Pfr) is strongly increased by a light pretreatment; however, if the increase of the PAL level or synthesis of anthocyanin is elicited by continuous far-red light (operating via phytochrome in the ‘High Irradiance Response’), effectiveness of light is strongly reduced by the same light pretreatment. This reduction of effectiveness is correlated with a decrease of total phytochrome (Ptot) caused by the light pretreatment. It is argued that the observations are compatible only with the ‘open phytochrome-receptor model’ as suggested by Schäfer (J. Mathem. Biol. 2, 41–56, 1975). The peaks of the time courses of the PAL levels under continous far-red light are located at 48 h after sowing and do not depend on the original level of phytochrome. The decrease of the PAL levels beyond 48 h after sowing takes place independently of phytochrome and of the actual level of PAL.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00387810
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  • 14
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    Phytochrome-mediated germination control of maize caryopses (1979)
    Springer
    Planta 146 (1979), S. 415-417 
    Details
    ISSN: 1432-2048
    Keywords: Germination (seeds) ; Osmotic stress ; Phytochrome ; Seed germination ; Zea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Maize caryopses sown in water germinate equally well either in darkness or under any light regime. However, when they are imbibed in mannitol solutions, continuous far-red light proves to be strongly inhibitory on the final germination as compared to darkness. Similar but less pronounced inhibition is also exhibited by continuous red or blue light. Intermittent far-red light can partially substitute for continuous far-red light in inhibiting maize caryopsis germination, and its effect is reversed to the intermittent red light level when red light is given immediately after each far-red illumination. These results are interpreted as a proof of existence and involvement of phytochrome in the germination control of maize caryopses, though its manifestation is realized only under osmotic stress.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00380854
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  • 15
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    Evidence for two photoreceptors controlling growth in de-etiolated seedlings (1979)
    Springer
    Planta 146 (1979), S. 545-550 
    Details
    ISSN: 1432-2048
    Keywords: Blue light ; Cucumis ; Hypocotyl growth ; Lactuca ; Lycopersicon ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of blue light on hypocotyl extension in de-etiolated seedlings of lettuce, cucumber and tomato was investigated under conditions which precluded the involvement of phytochrome. Small but highly inhibitory amounts of blue light were added to a high intensity background illumination from low pressure sodium lamps. A log-linear response for inhibition of hypocotyl extension against the blue light fluence rate was obtained for lettuce and cucumber, and inhibition in tomato was also related to the blue light fluence rate. The added blue light did not alter phytochrome photostationary state and its effect was independent of the total fluence rate. Growth inhibition by Pfr could be demonstrated in tomato and cucumber but not in lettuce. The results indicate that two photoreceptors may normally be involved in the control of seedling growth but their relative importance varies greatly between species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388830
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  • 16
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    Phytochrome and internode elongation in Chenopodium polyspermum L. the light fluence rate during the day and the end-of-day effect (1979)
    Springer
    Planta 146 (1979), S. 575-577 
    Details
    ISSN: 1432-2048
    Keywords: Chenopodium ; Day light fluence rate ; End-of-day effect ; Internode elongation ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The elongation of the fourth internode of fully green Chenopodium polyspermum L. is strongly stimulated by far-red light (FR) given at the end of the day. The end-of-day effect is more important when the plants had been cultivated for several days with a main light period of 140 Wm-2 than with a main light period of 85 Wm-2. There exists a quantitative relationship between the FR end-of-day effect mediated by phytochrome and the value of the light fluence during the day.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00388834
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  • 17
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    Identification and detection of carbamate pesticides in autopsy tissues using thin-layer chromatography (1979)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 294 (1979), S. 287-287 
    Details
    ISSN: 1618-2650
    Keywords: Nachw. von Carbamatpesticiden in biolog. Material ; Chromatographie, Dünnschicht
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00481656
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  • 18
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    Information content and equivocation of TLC identification procedures (1979)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 294 (1979), S. 361-365 
    Details
    ISSN: 1618-2650
    Keywords: Chromatographie, Dünnschicht ; Informationsgehalt und »Äquivokation«
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Summary A method has been developed for the calculation of information contents of one-channel identification procedures, which is especially applicable in the area of thin-layer chromatography (TLC). The method can be considered as a refinement of the one proposed by Massart and can easily be extended to the combination of one-channel procedures. In addition, the quantity ‘equivocation’, which is complementary to the information content, is introduced. As an example the best TLC-systems and the best combinations of two TLC-systems for the identification of DDT and twelve related compounds are selected by using information contents or equivocations. The results for the individual systems are compared with those obtained by Massart.
    Notes: Zusammenfassung Eine Methode zur Berechnung von Informationsgehalten von Einkanal-Identifizierungsverfahren wurde entwickelt, die besonders gut für das Gebiet der Dünnschicht-Chromatographie (DC) anwendbar ist. Sie kann als eine Verfeinerung der von Massart vorgeschlagenen Methode angesehen werden und kann auf einfache Weise für Kombinationen von Einkanal-Verfahren erweitert werden. Dabei wird der Begriff »Äquivokation«, komplementär zum Informationsgehalt, eingeführt. Als Beispiel wurden die besten DC-Systeme und Kombinationen von zwei DC-Systemen für die Identifizierung von DDT und zwölf verwandten Verbindungen unter Benutzung von Informationsgehalten oder »Äquivokationen« ausgewählt. Die Ergebnisse für die einzelnen Systeme werden mit den nach Massart gefundenen verglichen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00468523
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  • 19
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    TLC separation of potential antithyroid agents: N-aryl-N′-benzenesulphonylthiocarbamides (1979)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 294 (1979), S. 407-407 
    Details
    ISSN: 1618-2650
    Keywords: Trenn. von Arylbenzolsulfonylthiocarbamiden ; Chromatographie, Dünnschicht
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00468534
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  • 20
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    Universelle, halogenfreie DC-Fließmittel für Arzneisubstanzen (1979)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 295 (1979), S. 398-401 
    Details
    ISSN: 1618-2650
    Keywords: Analyse von pharmazeut. Produkten ; Chromatographie, Dünnschicht ; halogenfreie Fließmittelsysteme
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Mit 2 sauren, 2 basischen und einem neutralen Fließmittel mit unterschiedlichen Polaritäten und guten Trennleistungen konnten 151 von 157 geprüften Arzneisubstanzen mit hRf-Werten zwischen 10 und 90 chromatographiert werden.
    Notes: Summary Using two acidic, two alkaline and a neutral mobile phase of different polarities and high separation efficiencies 151 of 157 tested drug substances could be chromatographed with hRf-values between 10 and 90.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00539712
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  • 21
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    Zur Veränderung der Fluorescenzintensität polycyclischer Aromaten auf Dünnschichtplatten (1979)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 295 (1979), S. 24-29 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Polycyclen in Wasser ; Chromatographie, Dünnschicht ; Änderung der Fluorescenzintensität
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Nach der Verordnung über Trinkwasser und Brauchwasser für Lebensmittelbetriebe (Trinkwasserverordnung) vom 31. Januar 1975 müssen 6 Einzelverbindungen aus der Gruppe der polycyclischen aromatischen Kohlenwasserstoffe nachgewiesen werden. Das empfohlene Analysenverfahren basiert auf der Messung der Fluorescenz nach vorangegangener chromatographischer Trennung auf Adsorberschichten. Sowohl bei dieser Trennung als auch in der anschließenden Zeit verändert sich die Fluorescenzintensität einzelner Verbindungen z.T. beträchtlich. Systematische Untersuchungen an Kieselgel und an Mischschichten aus Aluminiumoxid und Acetylcellulose sollen die kritischen Punkte und die möglichen Fehlerquoten offen legen.
    Notes: Summary According to the regulation on the use of drinking and industrial water in food industry (Drinking Water Regulation) of 31 January, 1975, six individual compounds of the group of polycyclic aromatic hydrocarbons must be traced. The analytical procedure recommended is based on the measurement of fluorescence after preceding Chromatographic separation on adsorber layers. In the course of this separation and during the time that follows, the fluorescence intensity of various compounds changes, in some cases considerably. Systematic studies with silica gel and mixed layers of aluminium oxide and acetyl cellulose are intended to reveal critical points and possible ranges of error.
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    URL: http://dx.doi.org/10.1007/BF00490944
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    Zur Chromatographie von Metallchelaten (1979)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 297 (1979), S. 138-143 
    Details
    ISSN: 1618-2650
    Keywords: Trenn, der Metallchelate von 8-Mercaptochinolin ; Chromatographie, Dünnschicht
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung 8-Mercaptochinolin (Thiooxin) eignet sich zum Nachweis und zur Trennung von Metallen mit Hilfe der Dünnschicht-Chromatographie. Es bildet Neutralkomplexe definierter Zusammensetzung, die in wäßriger Phase schwerlöslich sind und in Chloroform extrahiert werden können. Fällung und Extraktion sind vom pH-Wert abhängig, wodurch eine Vortrennung der Metalle möglich ist. Beschrieben wird das dünnschicht-chromatographische Verhalten von 17 Metallchelaten. Die intensive Eigenfärbung der Komplexe erübrigt den Gebrauch von Anfärbereagentien. Die visuelle Nachweisgrenze der Metalle liegt zwischen 5 und 20 ng (Fluorescenzlöschung bei 254 nm). Spektralphotometrische Auswertung der Chromatogramme läßt eine Erniedrigung der Nachweisgrenze um etwa eine Zehnerpotenz erwarten.
    Notes: Summary 8-Mercaptoquinoline (thiooxine) is qualified for the detection and separation of metals by thin-layer chromatography. It forms neutral complexes in definite composition, which are insoluble in aqueous solution, but readily soluble in CHCl3. Precipitation and extraction are dependent on the pH value, whereby pre-separation of metals is possible. The thin-layer chromatographic behaviour of 17 metal chelates is described. No spraying is necessary for detection, since the complexes show different, characteristic colours. The visual detection limits of the metals are between 5 and 20 ng (fluorescence quenching at 254 nm). Spectrophotometric determination of the chromatograms lowers the detection limit by one power of ten.
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    URL: http://dx.doi.org/10.1007/BF00483594
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    Zur Chromatographie von Metallchelaten (1979)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 297 (1979), S. 144-147 
    Details
    ISSN: 1618-2650
    Keywords: Trenn. der Metallchelate von 1-Hydroxy-2-pyridinthion ; Chromatographie, Dünnschicht
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung 1-Hydroxy-2-pyridinthion (HPT) bildet mit den Metallen der 8. Nebengruppe des Periodensystems intensiv gefärbte Chelatkomplexe. Führt man die Reaktion in wäßriger Phase bei pH 7 aus, so werden alle Elemente der Gruppe erfaßt und als schwerlösliche Neutralkomplexe gefällt. Diese lassen sich mit Chloroform extrahieren und dünnschichtchromatographisch trennen. Die Nachweisgrenze der Metalle liegt zwischen 3 und 40 ng (Fluorescenzlöschung bei 254 nm). Spektralphotometrische Auswertung der Chromatogramme läßt eine Erniedrigung der Nachweisgrenze um eine Zehnerpotenz erwarten.
    Notes: Summary 1-Hydroxy-2-pyridinethione (HPT) forms highly coloured chelate complexes with the metals of the 8th sub-group. If the reaction is carried out in aqueous solution at pH 7, all elements of the group precipitate as insoluble neutral complexes. They can be extracted with CHCl3 and separated by thin-layer chromatography. The detection limit is between 3 and 40 ng (fluorescence quenching at 254 nm). Spectrophotometric determination of the chromatograms lowers the detection limit by one power of ten.
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    URL: http://dx.doi.org/10.1007/BF00483595
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    Rapid T.L.C. separation of some closely related potential antidiabetic guanylpyrazole nitrates (1979)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 298 (1979), S. 44-44 
    Details
    ISSN: 1618-2650
    Keywords: Trenn. von Guanylpyrazolnitraten ; Chromatographie, Dünnschicht
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00470050
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  • 25
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    Diphenylamin im DC-Fließmittel als universelles Detektionsmittel (1979)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 296 (1979), S. 45-45 
    Details
    ISSN: 1618-2650
    Keywords: Chromatographie, Dünnschicht ; Diphenylamin als Detektionsmittel
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00481170
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  • 26
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    Vollautomatische rechnergesteuerte Auswertung von Dünnschicht-Chromatogrammen (1979)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 296 (1979), S. 369-373 
    Details
    ISSN: 1618-2650
    Keywords: Chromatographie, Dünnschicht ; vollautomat. Auswertung von Chromatogrammen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Es werden vollautomatische DC-Photometer beschrieben, bei denen ein Rechner die optimale Positionierung der DC-Flecke im Meßspalt steuert. Es lassen sich nicht nur die normalen Meßkurven, sondern auch die gemäß der Kubelka-Munk-Funktion transformierten Kurven für die Auswertung heranziehen. Infolge der Steuerung des Wellenlängenantriebes lassen sich auch Zweiwellenlängentechniken simulieren. Für die Auswertung eignet sich auch die 1. Ableitung der Meßkurve. Die Genauigkeit und Reproduzierbarkeit wird gesondert nach Auftragefehler, Meßfehler, Positionierfehler und Fehler der Chromatographie ausgewiesen.
    Notes: Summary Automated photometers for evaluating TLC plates by an optimized positioning of the spots in respect to the centre of the light beam are described. The evaluation can be performed by using the original measuring curve or a transformed one in respect to the Kubelka-Munk function. Two-wavelength techniques can be simulated, for the monochromator is controlled by the computer. This method has an advantage in measuring not well separated spots. In this case also an evaluation of the first derivative is often successful. The accuracy and reproducibility is given in respect to the different sources of errors: spotting, measurement, positioning and chromatography.
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    URL: http://dx.doi.org/10.1007/BF00479975
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  • 27
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    Dünnschicht-chromatographische Trennung und Detektion von 14 1,4- bzw. 1,5-Benzodiazepin-Handelspräparaten und 17 Hauptmetaboliten (1979)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 294 (1979), S. 135-139 
    Details
    ISSN: 1618-2650
    Keywords: Trenn. von Benzodiazepinen ; Chromatographie, Dünnschicht ; Metaboliten
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Die DC-Trennung von 14 1,4- bzw. 1,5-Benzodiazepinen und 17 Hauptmetaboliten sowie die Detektion mit Bratton-Marshall-, Dragendorff- und Jodoplateat-Reagens werden beschrieben. Rf-Werte sowie die Reaktionen mit den Reagentien sind tabellarisch zusammengestellt und Biotransformationsschemata angegeben.
    Notes: Summary Experimental details for the separation are described and data are compiled for Rf-values and reactions with Bratton-Marshall, Dragendorff and iodoplateate-reagents. Biotransformation schemes for the individual benzodiazepines are presented.
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    URL: http://dx.doi.org/10.1007/BF00523935
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    Dünnschicht-chromatographischer Nachweis von IOXYNIL in biologischem Material (1979)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 294 (1979), S. 288-288 
    Details
    ISSN: 1618-2650
    Keywords: Nachw. von Ioxynil in Biolog. Material ; Chromatographie, Dünnschicht
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00481657
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    Alcohol separation by thin-layer chromatography. II (1979)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 295 (1979), S. 263-265 
    Details
    ISSN: 1618-2650
    Keywords: Trenn. von Alkoholen ; Chromatographie, Dünnschicht ; C1-C20, 6 Trennsysteme, Dinitrobenzoate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Summary Thin-layer chromatography on rice starch, maize starch, cellulose and talc makes possible the separation of the homologous series of 3,5-dinitrobenzoates of aliphatic alcohols C1 to C20. Six different solvent systems have been employed. Dependence of R f-values on the number of C-atoms on various supports is discussed.
    Notes: Zusammenfassung Mit Hilfe der Dünnschicht-Chromatographie auf Reisstärke, Maisstärke, Cellulose und Talk wurde die homologe Reihe der 3,5-Dinitrobenzoate von aliphatischen Alkoholen C1 bis C20 getrennt. Sechs verschiedene Fließmittel wurden benutzt. Die Abhängigkeit der R f-Werte von der Zahl der C-Atome auf verschiedene Schichten wird diskutiert.
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    URL: http://dx.doi.org/10.1007/BF00481489
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    Rapid T.L.C. separation of some closely related potential antineoplastic arylazopyrazoles (1979)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 295 (1979), S. 272-272 
    Details
    ISSN: 1618-2650
    Keywords: Trenn. von Arylazopyraolen ; Chromatographie, Dünnschicht
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00481495
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    Trennung und Identifizierung von 12 antimikrobiellen Verbindungen mittels Dünnschicht-Chromatographie (1979)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 294 (1979), S. 36-41 
    Details
    ISSN: 1618-2650
    Keywords: Trenn. von Antimikrobiellen Verbindungen ; Chromatographie, Dünnschicht
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Die Trennung und Identifizierung von halogenierten, vorwiegend aliphatischen antimikrobiellen Verbindungen, von Formaldehyd abgebenden Substanzen und von Sulfonsäure-Derivaten auf dünnschicht-chromatographischem Wege wird beschrieben. Die Trennung erfolgt auf einer Kieselgel-Cellulose-Mischschicht mit Äthylacetat/Methanol/10% Ammoniak (65+30+5) als Laufmittel. Zur Sichtbarmachung und zur Identifizierung sind mehrere Sprühmittel notwendig: äthanolischeMethylgelb-Lösung mit anschließender UV-Bestrahlung, Phenylhydrazinsulfonat und Natronlauge sowie Hydroxylamin/Eisen(III)-chlorid und Nitroprussidnatrium-Kaliumhexacyanoferrat (III) u. a. Anhand der verschiedenen Rf-Werte und der Anfärbungen lassen sich die antimikrobiellen Verbindungen rasch und einfach identifizieren.
    Notes: Summary The analysis of halogenated, mainly aliphatic compounds, of formaldehyde donors and of derivatives of sulphonic acid is described. The separation is carried out on plates coated with a mixed layer of silica gel and cellulose and a solvent mixture of ethylacetate/methanol/10% ammonia (65+30+5), whereas for the detection and identification several spray reagents have to be used: ethanolic solution of Methyl Yellow with subsequent UV irradiation, phenylhydrazine-4-sulphonic acid and sodium hydroxide, hydroxylamine/iron(III) chloride, sodium nitroprusside/potassium hexacyanoferrate(III) and others. The Rf-values and the colourations of the spots allow to identify the examined compounds rapidly and without further chemical procedures.
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    TLC separation of some inorganic ions on DCTA impregnated thin-layer plates (1979)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 294 (1979), S. 42-42 
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    ISSN: 1618-2650
    Keywords: Trenn. von anorg. Ionen ; Chromatographie, Dünnschicht ; DCTA-imprägniert
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
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    URL: http://dx.doi.org/10.1007/BF00694660
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    Identification of some closely related potential antidiabetic 4-arylhydrazono-N′-benzylsulphonyl-3-methyl-2-pyrazoline-5-ones by TLC (1979)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 294 (1979), S. 414-414 
    Details
    ISSN: 1618-2650
    Keywords: Identifizierung von Arylhydrazin-benzylsulfonylmethylpyrazolinonen ; Chromatographie, Dünnschicht
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
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    URL: http://dx.doi.org/10.1007/BF00468541
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    Separation and identification of closely related sulpha drugs by TLC on silica gel — calcium oxalate impregnated plates (1979)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 299 (1979), S. 207-207 
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    ISSN: 1618-2650
    Keywords: Analyse von Sulfonamiden ; Chromatographie, Dünnschicht
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00581378
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    Evidence of phytochrome involvement in the entrainment of the circadian rhythm of carbon dioxide metabolism in Bryophyllum (1978)
    Springer
    Planta 138 (1978), S. 271-278 
    Details
    ISSN: 1432-2048
    Keywords: Bryophyllum ; Circadian rhythm ; CO2 output ; Phytochrome ; Red light ; Rhythm (circadian)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The rhythm of carbon dioxide output in Bryophyllum leaves was entrained on exposure to 0.25 h of white light every 24 h. Entrainment also occurred on similar exposure to monochromatic radiation in spectral bands centred at 660 nm and, to a lesser extent, at 730 nm, but a band centred at 450 nm was without effect. A “skeleton” irradiation programme comprising two 0.25-h exposures to white light per 24 h also entrained the rhythm when the intervening dark periods were either 7.5 h and 16 h, or 10.5 h and 13 h. The rhythm disappeared when the two exposures were separated by 11.5-h and 12-h dark periods. Regular 0.25-h exposures to red light separated by 11.75-h periods of darkness also resulted in loss of the rhythm. Red/far-red reversibility was observed in irradiation schedules having either one or two exposures to red light daily. In the latter case, far-red reversal of the effects of one of the exposures to red light resulted in entrainment of the rhythm by the other, instead of abolition of the rhythm. The occurrence of distinct red/far-red reversibility suggests strongly that phytochrome is the pigment involved in entrainment of this rhythm by cycles of light and darkness.
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    Distorted phytochrome action spectra in green plants (1978)
    Springer
    Planta 138 (1978), S. 25-28 
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    ISSN: 1432-2048
    Keywords: Action spectrum ; Chlorophyll ; Phaseolus ; Photomorphogenesis ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Red light absorption by photosynthetic pigments screens stem tissue of Phaseolus vulgaris L. seedlings to such an extent that the most effective wavelength for the phytochrome induction control of stem growth is approximately 628 nm. Screening is greater in the first internode than in the hypocotyl. The consequences are that, compared with etiolated seedlings, much higher irradiances are required for phytochrome control in green plants, and the efficiency of 660 nm light is markedly reduced. Even very high exposures at 660 nm may not achieve appreciable photoconversion of P r to P fr.
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    Effects of narrow-beam irradiations with blue and far-red light on the timing of cell division in Adiantum gametophytes (1978)
    Springer
    Planta 138 (1978), S. 85-90 
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    ISSN: 1432-2048
    Keywords: Adiantum ; Blue-light effect ; Cell division ; Light, microbeam ; Photomorphogenesis ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Protonemata of the fern Adiantum capillusveneris L., grown as single-cell filaments under continuous red light, were irradiated with a narrow beam of blue light. Only irradiation of the region containing the nucleus induced cell division. Beams of 30 μm in width, which corresponds to the diameter of the nucleus, or wider, were equally effective; beams 10 μm wide or less were less effective. The results indicate that the nuclear region is the site of the blue- and near ultraviolet-light-absorbing pigment (PB-NUV) which mediates the timing effect of cell division. In contrast, the effect of a narrow beam of far-red (FR) light, which delays the onset of the blue-light-induced cell division, was found to be present along the entire length of the protonema cell, including the largely vacuolated basal region of the latter. Polarized FR light having the electrical vector parallel to the protonema axis was less effective than that vibrating in other directions. These observations support the hypothesis that the phytochrome controlling the timing effect is localized in the plasma membrane.
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    Phytochrome-mediated changes in the membrane potential of subepidermal cells of Lemna paucicostata 6746 (1978)
    Springer
    Planta 138 (1978), S. 133-136 
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    ISSN: 1432-2048
    Keywords: Lemna ; Membrane potential ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Light-stimulated transmembrane potential changes have been measured continuously after implantation of microelectrodes into subepidermal cells of the short-day plant Lemna paucicostata 6746. Irradiation for 5 min with white or red light caused a transient hyperpolarization. These potential changes could be suppressed with 10-6 M DCMU. Irradiation of DCMU-inhibited plants with far-red light for 5 min hyperpolarized the membrane potential, which thereafter was not changed by further far-red application. Consecutive red light irradiation for 5 min depolarized the membrane potential. The red/far-red reversibility of the potential changes (which could be repeated several times with a single plant) suggests the participation of phytochrome.
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    URL: http://dx.doi.org/10.1007/BF00391169
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    Light requirement, phytochrome and photoperiodic induction of flowering of Pharbitis nil Chois (1978)
    Springer
    Planta 141 (1978), S. 1-7 
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    ISSN: 1432-2048
    Keywords: Flowering ; Pharbitis ; Photomorphogenesis ; Photoperiodic induction ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract For dark-grown seedlings of Pharbitis nil capacity to flower in response to a single inductive dark period was established by 24 h white, far-red (FR) or ruby-red (BCJ) light and by a skeleton photoperiod of 10 min red (R)-24 h dark-10 min R. FR alone was ineffective without a brief terminal (R) irradiation, confirming that the form of phytochrome immediately prior to darkness is a crucial factor for flowering in Pharbitis. The magnitude of the flowering response was significantly greater after 24 h FR or white light (WL) (at 18° C and 27° C) than after two brief skeleton R irradiations, but the increased flowering response was not attributable to photosynthetic CO2 uptake because this could not be detected in seedlings exposed to 24 h WL at 18° C. Photophosphorylation could have contributed to the increased flowering response as photosystem I fluorescence was detectable in plants exposed to FR, BCJ, or WL, but there were large differences between flowering response and photosystem I capacity as indicated by fluorescence. We conclude that phytochrome plays a major role in photoresponses regulating flowering. There was no simple correlation between developmental changes, such as cotyledon expansion and chlorophyll formation during the 24-h irradiation period, and the capacity to flower in response to a following inductive dark period. Changes in plastid ultrastructure were considerable in light from fluorescent lamps and there was complete breakdown of the prolamellar body with or without lamellar stacking at 27 or 18° C, respectively, but plastid reorganization was minimal in FR-irradiated seedlings.
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    An in vitro association of soluble phytochrome with a partially purified organelle fraction from barley leaves (1978)
    Springer
    Planta 141 (1978), S. 71-76 
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    ISSN: 1432-2048
    Keywords: Hordeum ; Membranes ; Organelles ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Red light treatment in vitro increases the pelletability of phytochrome in homogenates of etiolated barley (Hordeum vulgare L. cv. ‘Julia’) leaves. When mixtures of soluble phytochrome (100,000 x g supernatant) and partially-purified organelles (Sephadex G-50 eluate) are irradiated the amount of pelletable phytochrome increases by a factor of two. Pre-irradiation treatments show that phytochrome in both components of the mixture must be in the Pfr form for increased pelletability to be observed. Once associated, photoreversion of Pfr to Pr does not result in decreased pelletability. The results are consistent with a non-artifactual in vitro association of soluble phytochrome to organelle membranes. One possible explanation is that Pfr molecules associate to form dimers.
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    URL: http://dx.doi.org/10.1007/BF00387747
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    Phytochrome photoreversibility: Empirical test of the hypothesis that it varies as a consequence of pigment compartmentation (1978)
    Springer
    Planta 141 (1978), S. 129-134 
    Details
    ISSN: 1432-2048
    Keywords: Avena ; Immunocytochemistry ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Phytochrome of oat (Avena sativa L., cv. Garry) coleoptile cells in the red-light-absorbing form, Pr, is diffusely distributed while after conversion to the far-red-light-absorbing form, Pfr, it is observed only in very small areas within the cell. Comparison of phytochrome photoversibility measurements to the distribution of the pigment within the cell indicates that the spectral assay is not influenced by the observed compartmentalization of the chromoprotein. However, the observed compartmentalization of phytochrome is correlated with a loss in spectrophotometrically detectable Pr.
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  • 42
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    Control by light of hypocotyl growth in de-etiolated mustard seedlings (1978)
    Springer
    Planta 141 (1978), S. 211-216 
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    ISSN: 1432-2048
    Keywords: Hypocotyl growth ; Photomorphogenesis ; Phytochrome ; Sinapis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract After sowing, mustard (Sinapis alba L.) seedlings were grown for 48 h in white light (25°C). These fully de-etiolated, green seedlings were used as experimental material between 48 and 72 (84) h after sowing. The question researched was to what extent control by light of hypocotyl elongation is due to phytochrome in these seedlings. It was found that the light effect on hypocotyl growth is very probably exerted through phytochrome only. In particular, we found no indication for the involvement of a specific blue light photoreceptor pigment.
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    URL: http://dx.doi.org/10.1007/BF00387891
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    Destruction and possible de novo synthesis of phytochrome in subcellular fractions of laminae from Avena sativa L. (1978)
    Springer
    Planta 141 (1978), S. 273-277 
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    ISSN: 1432-2048
    Keywords: Avena ; Etioplasts ; Mitochondria ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Phytochrome was determined in etiolated laminae of Avena sativaL. either without pretreatment or after 5 min of red irradiation followed by different periods of darkness (0–24 h). At given intervals laminae were homogenized and phytochrome was determined spectrophotometrically in the total homogenate and in purified etioplasts and mitochondria. Enhanced specific activity of phytochrome was found in all fractions after the irradiation in comparison to dark controls. Phytochrome destruction was observed in all fractions at the beginning of the subsequent dark period. Whereas the homogenate and the mitochondrial fraction showed a continuous destruction so that phytochrome reached a level far below that in etiolated plants, the phytochrome level in the plastid fraction reacheda minimum at 2 h with a subsequent increase beyond the dark level. This increase was most pronounced between 4 and 8 h after the red irradiation. The results are discussed in terms of the destruction and possible de novo synthesis of phytochrome that may be different in mitochondria and plastids.
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    Light effects upon dry lettuce seeds (1978)
    Springer
    Planta 144 (1978), S. 1-5 
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    ISSN: 1432-2048
    Keywords: Germination (seeds) ; Lactuca ; Phytochrome ; Seed germination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Germination of certain dry “seeds” (achenes) of Lactuca sativa L. cv. Grand Rapids was increased to ca. 75% after irradiation with 665 nm red light (R; 1x103 J m-2); this response was eliminated by far-red light (FR) following the R. The response of dry seeds required an order of magnitude more light than that of wetted seeds, and was not maximal until 48 h after irradiation. Other seeds, which could not be stimulated by R in dry state, showed a partial response after 10 min hydration. Irradiation of dry seeds (or seeds wetted 1 h) with FR (1x103 J m-2) reduced dark germination from 26% to 2%. Seeds dehydrated in an oven (60°C, 90 min) showed a decrease in germination if irradiated with R (1x105 J m-2) before wetting. The results show that phytochrome is present in dry lettuce seeds (and functional in some seed lots) prior to wetting; and that in other seed lots the molecule becomes functional within minutes after wetting the seeds. Transformation of the FR absorbing from of phytochrome (PFR) to the inactive from (PR) occurs at lower seed moisture content than the reverse reaction. It appears that dormancy in seeds ripened in sunlight might be assured during seed drying and maturation by the more effective transformation of PFR to PR than vice versa as phytochrome is dehydrated.
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    Spectrophotometric phytochrome measurements in light-grown Avena sativa L. (1978)
    Springer
    Planta 143 (1978), S. 309-313 
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    ISSN: 1432-2048
    Keywords: Avena ; Light-grown tissue ; Phytochrome ; San 9789
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Phytochrome was studied spectrophotometrically in Avena sativa L. seedlings that had been grown for 6 d in continous white fluorescent light from lamps. Greening was prevented through the use of the herbicide San 9789. When placed in the light, phytochrome (Ptot) decreased with first order kinetics (τ1/2 ≈ 2 h) but reached a stable low level (≈2.5% of the dark level) after 36 h. This concentration of phytochrome remained constant in the light and during the initial hours of a subsequent dark period, but increased significantly after a prolonged dark period. Evidence suggests that the constant pool of phytochrome in the light is achieved through an equilibrium between synthesis of the red absorbing (Pr) and destruction of the far-red absorbing form (Pfr) of phytochrome. It is concluded that the phytochrome system in light-grown oat seedlings is qualitatively the same as that known from etiolated monocotyledonous seedlings, but different than that described for cauliflower florets.
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    URL: http://dx.doi.org/10.1007/BF00392003
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    The circadian rhythm in Bryophyllum leaves: Phase control by radiant energy (1978)
    Springer
    Planta 143 (1978), S. 323-328 
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    ISSN: 1432-2048
    Keywords: Bryophyllum ; Circadian rhythm ; Photocontrol ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A 4-h exposure to white light from fluorescent lamps can shift the phase of the rhythm of CO2 output in leaves of Bryophyllum fedtschenkoi Hamet & Perr. otherwise kept in continuous darkness. The position in the cycle at which irradiation occurs determines the magnitude and direction of the phase shift. Red and white light induce similar advances or delays in the phase, but blue and far-red irradiation have no effect. Far-red irradiation given simultaneously with, or immediately after, exposure to red light, modifies the phase-shift induced by red light alone. Radiation in the red and far-red regions of the spectrum interacted in several experimental régimes, but complete red/far-red reversibility was not observed. The evidence suggests that phytochrome is the receptor molecule involved in the induction of phase-shifts by light.
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    Action spectra for chromatic adaptation in the blue-green alga Fremyella diplosiphon (1978)
    Springer
    Planta 143 (1978), S. 233-239 
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    ISSN: 1432-2048
    Keywords: Adaptation (chromatic) ; Chromatic adaptation ; Cyanophyta (cyanobacteria) ; Phycobiliproteins ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Action spectra for chromatic adaptation in Fremyella diplosiphon Drouet have been determined using techniques previously described. Action maxima are at 540 nm, with a half-band width of 80 nm, for induction of phycoerythrin synthesis (green action) and at 650 nm, with a half-band width of 90 nm, for reversal of induction of phycoerythrin synthesis (red action). The red-action spectrum includes a secondary action band centered at ca. 360 nm. Red and green action overlap from 570 to 590 nm with an isosbestic point in the vicinity of 580 nm. Shoulders are present at 520 and 630 nm. Red light is more active than green light. The 540:650-nm quantum effectiveness ratio is 1:7. There is relatively little action of either kind in the blue. The 387:540 nm and 460:650-nm quantum effectiveness ratios are zero. These results contrast strongly with previous determinations in the same organism, with major activity indicated in the blue; they are consistent with the control of photomorphogenesis in the Cyanophyta by a master pigment, analogous to phytochrome.
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    Light requirement, phytochrome and photoperiodic induction of flowering of Pharbits nil Chois (1978)
    Springer
    Planta 141 (1978), S. 9-14 
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    ISSN: 1432-2048
    Keywords: Flowering ; Pharbitis ; Photoperiodic induction ; Phytochrome ; Spectrophotometry (phytochrome)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The low chlorophyll content of cotyledons of Pharbitis nil grown for 24 h in far-red light (FR) or at 18° C in white light from fluorescent lamps (WL) allows spectrophotometric measurement of phytochrome in these tissues. The Δ(ΔA) measurements utilize measuring beams at 730/802 nm and an actinic irradiation in excess of 90 s. The constancy of the relationship between phytochrome content and sample thickness confirms that, under these conditions of measurement, a true maximum phytochrome signal was obtained. These techniques have been used to follow changes in the form and amount of phytochrome during an inductive dark period for flowering. Following exposure to 24h WL at 18° C with a terminal 10 min red (R), Pfr was lost rapidly in darkness and approached zero in less than 1 h; during this period there was no change in the total phytochrome signal. Following exposure to 24 h FR with a terminal 10 min R, Pfr approached zero in 3 h, and the total phytochrome signal decreased by about half. The relevance of these changes to photoperiodic time measurement is discussed.
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    Light requirement, phytochrome and photoperiodic induction of flowering of Pharbitis nil Chois (1978)
    Springer
    Planta 141 (1978), S. 15-22 
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    ISSN: 1432-2048
    Keywords: Flowering ; Pharbitis ; Photoperiodic induction ; Phytochrome ; Spectrophotometry (phytochrome)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract During an inductive dark period for flowering, changes in phytochrome have been followed in cotyledons of Pharbitis nil seedlings using a spectrophotometric and a physiological technique. There was agreement between the two techniques, both indicating a rapid lowering of the levels of the far-red-absorbing form of the pigment (Pfr) during the first 30–90 min of darkness. Thus, Pfr could provide the cue which signals the beginning of darkness and the length of the critical dark period was, in some instances, correlated with the time of Pfr disappearance. However, there was no correlation with another indicator of photoperiodic time measurement, the time in darkness at which a brief night interruption becomes inhibitory to flowering. These results imply that the transition from light to darkness is not signalled by the disappearance of Pfr. However, the involvement of other photoreceptors seems unlikely. Thus, some mode of phytochrome action other than a simple Pfr-mediated response may be involved in the light/dark-sensing reactions of photoperiodism
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    Phytochrome-mediated control of grana and stroma thylakoid formation in plastids of mustard cotyledons (1978)
    Springer
    Planta 141 (1978), S. 191-198 
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    ISSN: 1432-2048
    Keywords: Chlorophyll ; Chloroplasts ; Grana ; Phytochrome ; Sinapis ; Thylakoids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The etioplast»chloroplast transition in the cotyledons of mustard seedlings (Sinapis alba L.) has been studied by electron microscopy. It was found that the active form of phytochrome, established by a red light pulse pretreatment, increases the initial rate and eliminates the lag of grana and stroma thylakoid formation after the onset of white light 60 h after sowing. The effect of a pretreatment with 15 s red light pulses is fully reversible by 756 nm light pulses. This reversibility is lost within 5 min. Evidence is presented which suggests that the time course of grana and stroma thylakoid formation is not correlated with the time course of the dispersal of the prolamellar body. The different functions of phytochrome and chlorophyll in controlling thylakoid formation are discussed.
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    Investigations on the role of ethylene in phytochrome-mediated photomorphogenesis (1978)
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    Planta 142 (1978), S. 119-122 
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    ISSN: 1432-2048
    Keywords: Chlorophyll ; Ethylene ; Phenylalanine ammonia-lyase ; Phytochrome ; Sinapis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The concept (Burg, 1973) that ethylene mediates the action of phytochrome in seedling photomorphogenesis was tested in the intact mustard (Sinapis alba L.) seedling. The effect of exogenous ethylene (100 μl l−1) on five distinct, phytochrome-mediated photoresponses of the cotyledons was investigated. It was found that anthocyanin contents (see Bühler et al., 1978) and phenylalanine ammonia-lyase levels (EC 4.3.1.5) are strongly reduced by ethylene while the capacity of chlorophyll synthesis is considerably enhanced. Levels of glutathione reductase (EC 1.6.4.2) and pools of photoconvertible protochlorophyll(ide) are unaffected by ethylene. It is concluded that these findings are incompatible with the idea that ethylene plays the role of a mediator in phytochrome-induced photomorphogenesis.
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    The relationship between phytochrome-photoequilibrium and Development in light grown Chenopodium album L. (1978)
    Springer
    Planta 142 (1978), S. 187-193 
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    ISSN: 1432-2048
    Keywords: Chenopodium ; Far-red ; Photoequilibrium ; Phytochrome ; Stem extension
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Chenopodium album seedlings were grown in light environments in which supplementary far-red light was mixed with white fluorescent light during various parts of the photoperiod. Both the logarithmic rate constant of stem extension and the leaf dry weight: stem dry weight ratio were linearly related to estimated phytochrome photoequilibrium (ϕ) in each treatment regime. These data are taken to be indicative of a functional link between phytochrome and development in the green plant. A layer of chlorophyllous tissue only affected the linearity between calculated ϕ and the logarithmic stem extension rate at high chlorophyll concentrations, whilst even low concentrations-equivalent to the levels found in stem tissue-caused a significant shift in measured ϕ. End-of-day supplementary far-red (FR) light induced between 0–35 per cent of the response elicited by all-day supplementary FR, whilst daytime supplementary FR (with a white fluorescent light end-of-day treatment) induced approximately 90 per cent. The ecological significance of this difference is discussed with respect to shade detection.
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    Boron — Key element in the actions of phytochrome and gravity? (1978)
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    Planta 143 (1978), S. 109-111 
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    ISSN: 1432-2048
    Keywords: Boron ; Fluorescein ; Gravity ; Phytochrome ; Vigna
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Hypocotyl sections from dark-grown mung bean (Vigna radiata (L.) Wilczek) seedlings grown without boron exhibit little or no increase in the translocation of fluorescein in phloem cells following gravitational stimulation or red irradiation. In contrast, pretreatment of the hypocotyl sections with low concentrations of boric acid before red irradiation or gravitational stimulation results in a rapid increase in fluorescein movement. Mung bean root tips without boron fail to remain attached to a negativelycharged glass surface after several minutes of red irradiation. A low boron concentration in the solution induces tip attachment. The suggestion is made that boron is required to stabilize a positive electrostatic charge in the plasma membrane that is generated by the actions of phytochrome and gravity.
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    Phytochrome involvement in the induction of resistance to dark abscission by malformin (1978)
    Springer
    Planta 141 (1978), S. 311-314 
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    ISSN: 1432-2048
    Keywords: Abscission resistance ; Malformin ; Phaseolus ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The active portion of the visible spectrum which is required for malformin to produce leaves which are resistant to dark abscission from cuttings of Phaseolus aureus is red light. Abscission resistance was partially to almost completely lost by far irradiation prior to dark incubation. Although Ethrel, an ethylene releasing compound, stimulated dark abscission of resistant and control leaves, resistance was not lost because control leaves always abscised at a greater rate. The participation of phytochrome in the induction of abscission resistance by malformin is indicated.
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    Investigations on the role of ethylene in phytochrome-mediated photomorphogenesis (1978)
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    Planta 142 (1978), S. 109-117 
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    ISSN: 1432-2048
    Keywords: Anthocyanin synthesis ; Ethylene ; Phytochrome ; Sinapis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The etiolating, intact mustard (Sinapis alba L.) seedling exhibits a distinct temporal pattern of ethylene production. Light, operating through phytochrome, increases the rate of ethylene production without changing the pattern. Ethylene production of the isolated plant parts (segments), added together, exceed the production of the intact system even if the wound effect is taken into account. There is no significant light effect on ethylene production of the segments. Phytochrome-mediated anthocyanin synthesis in the cotyledons is inhibited by ethylene. The responsiveness towards ethylene of the anthocyanin producing metabolic chain is decreased by phytochrome. As anthocyanin synthesis is only partly inhibited under saturating ethylene concentrations in the atmosphere around the seedlings (100 μl l−1), a twofactor analysis becomes feasible. This analysis leads to the result that phytochrome and ethylene show multiplicative behavior, meaning that phytochrome and ethylene act on the same metabolic sequence (leading to anthocyanin) but independently of each other, and at different sites. Therefore, the hypothesis that ethylene mediates the action of phytochrome in anthocyanin synthesis and photomorphogenesis in general appears to be inapplicable.
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    Spectral characteristics of phytochrome in vivo and in vitro (1978)
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    Planta 142 (1978), S. 225-228 
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    ISSN: 1432-2048
    Keywords: Hordeum ; Phytochrome ; Receptor site
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The absorption maximum of the far-red absorbing form of phytochrome in the difference spectrum for phototransformation (Pfr λmax) was investigated in vivo and in in vitro pellets from dark grown Hordeum vulgare L. primary leaves. Exposure of pellets in Honda medium from tissue pre-irradiated with red light to far red light gave a Pfr λmax of 734 nm, a slightly longer wavelength than was seen in vivo (730 nm). After incubation as the red absorbing form of phytochrome (Pr) for 2 h at 0° C irradiation with red light showed that Pfr λmax had shifted to shorter wavelength (716 nm) in Honda medium. Further incubation as Pfr for 2 h at 0° C and irradiation with far red light showed that Pfr λmax had shifted to longer wavelength (726 nm). Similar shifts were also seen in other media, although the peak positions were different. Phytochrome remained pelletable throughout these experiments and Pfr λmax is compared to that of soluble phytochrome in similar media. The results are interpreted as indicating changes in molecular environment of the putative phytochrome membrane receptor site and that Pfr λmax can be used to probe the nature of this binding.
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    Quantitative dünnschicht-chromatographische Bestimmung von aromatischen Aminen nach der Remissionsmethode (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 292 (1978), S. 381-384 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Aminen, aromat. ; Chromatographie, Dünnschicht ; Remissionsmessung
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Es wird eine Methode zur quantitativen Bestimmung von aromatischen Aminen beschrieben. Diese werden dünnschicht-chromatographisch auf Kieselgel 60 F254 Fertigplatten in den Laufmitteln n-Propanol/Methanol/Wasser/Eisessig (65∶15∶15∶20; Vol.) oder n-Butanol/Wasser/Eisessig (66∶17∶17; Vol.) von den Begleitstoffen abgetrennt. Die getrennten Aminflecke werden entweder im Ultraviolett-Bereich oder nach Diazotierung im sichtbaren Bereich mit dem Chromatogramm-Spektralphotometer PMQ II nach der Remissionsmethode gemessen und anhand der auf derselben Platte aufgestellten Eichgerade ausgewertet. Die quantitative Bestimmung wird anhand der beiden Amine 5-Amino-2,4,6-trijod-isophthalsäurebis-(2,3-dihydroxy-propyl-N-methylamid) (I) und 2,4,6-Trijod-3-aminobenzoesäure (II) in Röntgenkontrastmittelpräparaten (RKP) demonstriert. Sowohl im UV- als auch im sichtbaren Bereich, läßt sich in 3000 μg RK-Säure (RKS) noch 0,1 μg freies aromatisches Amin, das sind 0,003%, bezogen auf die eingesetzte Menge RKS, mit einer maximalen relativen Standardabweichung von 4% bestimmen. Diese Methode ist spezifischer und empfindlicher als die der Diazotierung in Lösung.
    Notes: Summary The aromatic amines are separated from impurities by thin-layer chromatography on silica gel 60 F254 precoated plates in the mobile solvents n-propanol/methanol/water/glacial acetic acid (65∶15∶15∶20; vol.) or n-butanol/water/glacial acetic acid (66∶17∶17; vol.). The separated amine spots are measured by the remission method by means of the TLC densitometer PMQ II, either in the u.v. region or, after diazotization, in the visible region, and evaluated on the basis of the linear calibration curve set up on the same plate. Quantitative determination is demonstrated by means of the two amines, 5-amino-2,4,6-triiodoisophthalic acid-bis-(2,3-dihydroxypropyl-N-methylamide) (I) and 2,4,6-triiodo-3-aminobenzoic acid (II), in radiopaque contrast media (RCM). Both in the u.v. and visible regions, it is still possible to determine 0.1 μg of free aromatic amine in 3000 μg of radiopaque contrast medium acid (RCA), or 0.003% in terms of the amount of RCA used, with a maximum relative standard deviation of 4%. This method is more specific and more sensitive than that of diazotization in solution.
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    Analyse von Desodorantien in kosmetischen Präparaten (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 293 (1978), S. 135-137 
    Details
    ISSN: 1618-2650
    Keywords: Analyse von Desodorantien, Phenolderivaten in Kosmetika ; Chromatographie, Dünnschicht
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Phenolische Desodorantien in kosmetischen Präparaten lassen sich durch Umsetzung mit Echtrotsalz Al (Anthrachinondiazonium-chlorid-1) in wäßrig-alkalischer Lösung, Extraktion des gebildeten Azofarbstoffes mit einem organischen Lösungsmittel und anschließender Dünnschicht-Chromatographie schnell identifizieren. In den meisten Fällen ist die Abhängigkeit zwischen der Extinktion der Farbstofflösung und der eingesetzten Menge des betreffenden Desodorants linear, wodurch dann auch quantitative Bestimmungen möglich sind.
    Notes: Summary Phenolic compounds, used as deodorants in cosmetic products can be identified quickly by reaction with anthraquinonediazonium chloride-1 (Echtrotsalz Al) in aqueous alkaline medium, extraction of the azo dye with an organic solvent and separation by thinlayer chromatography. The relation between the absorbance of the coloured solution and the concentration of the deodorant concerned is generally linear; in these cases quantitative determinations are always possible.
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    Trennung und Nachweis von N-Heterocyclen auf CuSO4-imprägnierten Schichten (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 291 (1978), S. 366-368 
    Details
    ISSN: 1618-2650
    Keywords: Nachw. von N-Heterocycl. Verbindungen ; Chromatographie, Dünnschicht ; CuSO4-imprägnierte Schichten, Fluorescenz
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Durch die charakteristischen Fluorescenzfarben und die Fluorescenz-Thermochromie der CuJ-Komplexe lassen sich N-Heterocyclen auf Chromatogrammen meist einwandfrei identifizieren. Eine wesentliche Vereinfachung wird durch die Verwendung CuSO4-imprägnierter Kieselgelschichten erreicht. Die Nachweisempfindlichkeiten insbesondere bei tiefer Temperatur ändern sich im Vergleich zur ursprünglichen Technik kaum.
    Notes: Summary N-Heterocycles can usually be identified with certainty through the characteristic fluorescence colours and fluorescence-thermochromism of the CuI-complexes. The use of CuSO4-impregnated silica gel layers facilitates the procedure considerably, and scarcely reduces the sensitivity of detection, particularly at low temperature.
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    TLC separation of some inorganic ions on p-toluidine impregnated layers (1978)
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    Fresenius' Zeitschrift für analytische Chemie 292 (1978), S. 415-416 
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    ISSN: 1618-2650
    Keywords: Trenn. von Anorgan. Ionen ; Chromatographie, Dünnschicht ; mit p-Toluidin imprägnierte Schichten
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
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    A simple procedure for the chromatographic isolation and determination of prostaglandins from human seminal plasma (1978)
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    Fresenius' Zeitschrift für analytische Chemie 293 (1978), S. 45-48 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Prostaglandinen in Sperma ; Chromatographie, Dünnschicht
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Summary A relatively simple procedure for the isolation and determination of the prostaglandins present in human seminal fluid is described. It involves preliminary chromatographic purification of these compounds from the major non-prostaglandin impurities followed by their total elution in one solvent (one-step elution). The prostaglandins thus obtained were almost free from other lipids and were further resolved into prostaglandin-groups and individual prostaglandins by repeated thin-layer chromatography. Data are also presented for prostaglandin contents of fresh semen samples from five individuals and results compared with those from the stored samples.
    Notes: Zusammenfassung Das Verfahren umfaßt eine chromatographische Abtrennung der Verbindungen von den hauptsächlichsten Verunreinigungen und die Gesamtelution mit einem Lösungsmittel. Die von anderen Lipiden fast völlig freien Prostaglandine werden durch wiederholte Dünnschicht-Chromatographie in Gruppen und Einzelverbindungen getrennt. Werte werden angegeben über die Prostaglandingehalte von frischem im Vergleich zu gelagertem Sperma.
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    Trennung und Identifizierung von Inhaltsstoffen der HCH-Salbe „Antiskabiosum“ an Fertigfolien für die Dünnschicht-Chromatographie (Reinheitsprüfung) (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 292 (1978), S. 237-237 
    Details
    ISSN: 1618-2650
    Keywords: Analyse von HCH-Salbe ; Chromatographie, Dünnschicht ; Fertigfolien
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00491816
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    T.L.C. separation of some closely related potential antidiabetic 3,5-dimethyl-4-arylazoisoxazoles (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 292 (1978), S. 238-238 
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    ISSN: 1618-2650
    Keywords: Trenn. von Dimethylarylazoisoxazolen ; Chromatographie, Dünnschicht
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
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    URL: http://dx.doi.org/10.1007/BF00491817
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    Untersuchung der Biotransformation von Pemolin im menschlichen Organismus (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 289 (1978), S. 110-111 
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    ISSN: 1618-2650
    Keywords: Identifizierung von Pemolin-Metaboliten im Harn ; Chromatographie, Dünnschicht ; UV-, IR-, Massenspektren
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
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    URL: http://dx.doi.org/10.1007/BF00443957
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    Bestimmung von Trimethylzinnverbindungen in Dimethylzinn-di(thioglykolsäureisooctylester) (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 289 (1978), S. 285-286 
    Details
    ISSN: 1618-2650
    Keywords: Best. von Trimethylzinnverbindungen in Dimethylzinn-di(thioglykolsäureisooctylester) ; Chromatographie, Dünnschicht
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
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    URL: http://dx.doi.org/10.1007/BF00440214
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    TLC separation of some inorganic ions (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 290 (1978), S. 46-46 
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    ISSN: 1618-2650
    Keywords: Trenn. von Ionen, anorgan ; Chromatographie, Dünnschicht
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
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    URL: http://dx.doi.org/10.1007/BF00468863
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    Separation of rhodium and iridium by anion-exchange thin-layer chromatography (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 290 (1978), S. 46-47 
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    ISSN: 1618-2650
    Keywords: Trenn. von Rhodium und Iridium ; Chromatographie, Dünnschicht ; Anionenaustausch
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
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    URL: http://dx.doi.org/10.1007/BF00468864
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    TLC-separation of potential antineoplastic agents: Ethyl-2, 3-dioxobutyrate-2-arylhydrazones (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 290 (1978), S. 48-48 
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    ISSN: 1618-2650
    Keywords: Trenn. von Äthyl-2,3-dioxobutyrat-2-arylhydrazonen ; Chromatographie, Dünnschicht ; Antineoplastica
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
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    URL: http://dx.doi.org/10.1007/BF00468866
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    Automatische Fluorimetrie von Mikro-Dünnschicht-Chromatogrammen (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 290 (1978), S. 133-134 
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    ISSN: 1618-2650
    Keywords: Analyse von Aminosäuren ; Chromatographie, Dünnschicht ; automat. F luorimetrie
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
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    URL: http://dx.doi.org/10.1007/BF00482294
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    Identification of a new metabolite of digitoxin by radio-thin-layer-chromatography (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 290 (1978), S. 157-157 
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    ISSN: 1618-2650
    Keywords: Nachw. von Digitoxinmetaboliten in Harn ; Chromatographie, Dünnschicht ; Radio
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00482314
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    Semiquantitative estimation of the mycotoxins citrinin, Ochratoxin A, Aflatoxin M1 and penicillic acid on thin-layer chromatograms with a grey scale (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 293 (1978), S. 138-140 
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    ISSN: 1618-2650
    Keywords: Best. von Mycotoxinen, Citrinin, Ochratoxin A, Aflatoxin M1, Penicillinsäure ; Chromatographie, Dünnschicht ; Graukeil
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Summary Spots of citrinin, ochratoxin A, aflatoxin M1 and penicillic acid on thin-layer chromatograms can be semi-quantitatively estimated by using a grey scale, commonly used in photo laboratories. The detection limits are 0.01 μg for citrinin, 0.006 μg for ochratoxin A, 0.002 μg for aflatoxin M1 and 0.01 μg for penicillic acid. Standard curves for these mycotoxins are given.
    Notes: Zusammenfassung Mit Hilfe eines in Photolabors gebräuchlichen Graukeils können dünnschicht-chromatographische Flecke der obengenannten Mycotoxine halbquantitativ bestimmt werden. Die Nachweisgrenzen betragen 0,01 μg für Citrinin, 0,006 μg für Ochratoxin A, 0,002 μg für Aflatoxin M1 und 0,01 μg für Penicillinsäure. Eichkurven werden angegeben.
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    TLC separation of closely related aromatic amines II (1978)
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    Fresenius' Zeitschrift für analytische Chemie 291 (1978), S. 221-221 
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    ISSN: 1618-2650
    Keywords: Trenn. von Aminen, aromat. ; Chromatographie, Dünnschicht
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
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    URL: http://dx.doi.org/10.1007/BF00480695
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    Identification of some 2[2-mercapto (or substituted mercapto)-4-thiazolyl] benzothiazoles by thin-layer chromatography (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 291 (1978), S. 224-224 
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    ISSN: 1618-2650
    Keywords: Nachw. von Mercaptothiazolyl-benzothiazolen ; Chromatographie, Dünnschicht
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
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    URL: http://dx.doi.org/10.1007/BF00480698
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    Untersuchungen zur quantitativen Bestimmung der Seltenen Erden nach dünnschicht-chromatographischer Trennung mit bekannten und neuen Laufmittelsystemen (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 291 (1978), S. 328-331 
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    ISSN: 1618-2650
    Keywords: Trenn. von Seltenen Erden von Uran ; Photometrie ; Chromatographie, Dünnschicht ; Diäthyläther/Tetrahydrofuran/Bis-(2-äthylhexylphosphat)/HNO3
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Mit dem Laufmittel Diäthyläther/Bis-(2-äthylhexylphosphat) (HDEHP)/Salpetersäure (100∶1∶3,5) können alle Seltenen Erden (S.E.) dünnschicht-chromatographisch (DC-Trennung) getrennt werden. Durch Variation von HDEHP und HNO3 ist es möglich, entweder die Rf-Werte der schweren oder leichten S.E. auseinander zu ziehen. Eine weitere DC-Trennung der S.E. gelingt mit dem Laufmittel Diäthyläther/Tetrahydrofuran/HDEHP/ HNO3. Nach der DC-Trennung werden die einzelnen S.E. auf zwei Wegen quantitativ bestimmt: a) Durch Auswertung der Fleckengröße, b) photometrisch nach Abkratzen der Flecken mit Neothorin. Als Analysen-Beispiel wird die Bestimmung von S.E. im ppm-Bereich in Spilitischem Schalstein gezeigt. Die relativen Standardabweichungen sind befriedigend.
    Notes: Summary All rare earths can be separated by TLC with the eluent diethyl ether/bis-(2-ethylhexylphosphate) (HDEHP)/nitric acid (100∶1∶3.5). It is possible to increase the R f-differences between either the heavy or the light rare earths by variation of HDEHP and HNO3. A further separation can be obtained by using diethyl ether/THF/HDEHP/HNO3 as an eluent. After TLC separation the rare earths can be determined quantitatively in two ways: (a) by evaluation of spot area, (b) by photometry with Neothorin as indicator after scraping the spots. The determination of rare earths in the ppm range in spilites is shown as an example. The relative standard deviations are satisfactory.
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    Radiochemical assay of prostaglandins, thromboxane, prostaglandin endoperoxides, 12-hydroxyeicosatetraenoic acid (HETE) and 12-hydroxyheptadecatrienoic acid (HHT) formed by human platelets from arachidonic acid (1978)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 292 (1978), S. 228-232 
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    ISSN: 1618-2650
    Keywords: Analyse von Prostaglandinen, Thromboxan, Prostaglandinendoperoxiden, 12-Hydroxyeicosantetraensäure, 12-Hydroxyheptadecatriensäure aus Thrombocyten ; Chromatographie, Dünnschicht ; gebildet aus Arachidonsäure, radiochemisch
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Summary A three-step thin-layer chromatographic procedure is described for the isolation and measurement of the various compounds formed by human platelets from radioactive arachidonic acid. The data presented show that other unknown compounds may also be formed in substantial amounts. The method can be used on a routine basis by combining a two-dimensional thin-layer chromatographic separation with an additional TLC for a complete resolution of the prostaglandins and other products.
    Notes: Zusammenfassung Ein dreistufiges TLC-Verfahren wird beschrieben zur Trennung und Bestimmung der verschiedenen Verbindungen, die durch Thrombocyten aus radioaktiver Arachidonsäure gebildet werden. Die vorliegenden Ergebnisse zeigen, daß außer den oben genannten noch weitere unbekannte Verbindungen in wesentlichen Mengen gebildet werden können. Durch Kombination einer dreidimensionalen TLC mit einer weiteren TLC-Trennung kann eine vollständige, routinemäßige Analyse der Prostaglandine und anderen Verbindungen durchgeführt werden.
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    URL: http://dx.doi.org/10.1007/BF00491813
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    Phytochrome and phosphotungstate-chromate-positive vesicles from Cucurbita pepo L. (1977)
    Springer
    Planta 133 (1977), S. 169-177 
    Details
    ISSN: 1432-2048
    Keywords: Cell fractionation ; Cucurbita ; Histochemical staining ; Phosphotungstate-chromate staining ; Phytochrome ; Plasma membrane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The phosphotungstic acid-chromic acid (PTA-CrO3) stain, putatively specific for the plasma membrane of plants, has been used in an attempt to monitor the distribution of this membrane in a 20,000 x g particulate fraction from Cucurbita hypocotyl hooks. On discontinuous sucrose gradients, the relative distributions of the phytochrome and PTA-CrO3-positive vesicles present in this fraction appear to be correlated. When intact tissue is stained, however, other components, in addition to the plasma membrane, react positively to the stain. These components include prolamellar-body membranes, lipid droplets, and ribosomes. This lack of specificity calls into question the reliability of the technique for the unequivocal identification and accurate quantitation of plasma-membrane fragments in isolated particulate fractions. The present data do not, therefore, provide unambiguous evidence that phytochrome is associated with plasma membrane in tissue homogenates from Cucurbita.
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    Influence of chlorophyll content on phytochrome measurements in turnip cotyledons (1977)
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    Planta 134 (1977), S. 11-16 
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    ISSN: 1432-2048
    Keywords: Brassica ; Chlorophyll ; Cotyledons ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Phytochrome determinations at 730/800 nm were performed on de-etiolated turnip (Brassica rapa L.) cotyledons in which chlorophyll (Chl) content had been reduced experimentally to varying degrees by pre-treatment with high temperature (HT), or transfer to 3% ethanol. The magnitude of detectable phytochrome depended on Chl content, showing a linear relationshop in vivo. The results were confirmed by an in vitro experiment where, however, the correlation was exponential. An attempt is made to illustrate phytochrome decay in continuous blue or red light after corretion for Chl interference. To overcome the possible objection that the higher Δ(ΔA) measured in continuous light after HT pre-treatment could be caused by a reduced rate of destruction, apparent re-synthesis following red light was measured after several hours of darkness during which time the HT effect was lost. Under these conditions HT pre-treated samples display a more realistic magnitude of apparent new synthesis and make correlation with a physiological response possible.
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    Radiation-induced appearance of phytochrome in soybean cell suspensions (1977)
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    Planta 134 (1977), S. 57-59 
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    ISSN: 1432-2048
    Keywords: Cell culture ; Clycine ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract No phytochrome can be detected spectrophotometrically in soybean (Glycine max (L.) Merr. cv. Mandarin) cell suspensions grown in darkness. White light from a tungsten filament lamp and a band of far-red light have been found to induce the appearance of phytochrome in them. Red light failed to bring about phytochrome increase and destroyed the phytochrome induced by far-red light.
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    Chlorophyll interference with phytochrome measurement (1977)
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    Planta 135 (1977), S. 119-123 
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    ISSN: 1432-2048
    Keywords: Chlorophyll ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Measurements of phytochrome by Δ (ΔA725–815 nm) were completely suppressed at chlorophyll concentrations of the order of 20–40 μg g-1 f.wt. in vivo and 37 μg cm-3 in vitro, and the readings were reduced by 50% at only 12 μg cm-3 in vitro. At these concentrations of chlorophyll in aqueous methanol, the loss of phytochrome signal in vitro appeared to be due to failure of phytochrome photoconversion rather than to interference with ΔA measuremebt by chlorophyll fluorescence in the 125/815 nm measuring beam.
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    Photoreception and photoresponses in the radish hypocotyl (1977)
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    Planta 136 (1977), S. 125-129 
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    ISSN: 1432-2048
    Keywords: Blue-light-response ; Elongation ; High-irradiance-response ; Photomorphogenesis ; Phytochrome ; Raphanus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In etiolated hypocotyls of Raphanus sativus L. the growth responses to continuous red, far-red and blue light have been distinguished on the bases of photoreceptive sites and regions of physiological response. Blue light appeared to retard a fairly mature stage of elongation, acting immediately and directly on the cells irradiated. Far-red light caused a marked inhibition of all stages of elongation after a lag period, and the stimulus could be transmitted from the hook region. The effect of red light was complex and consisted of one promotive and two inhibitory responses.
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    Action spectra for the inhibition of growth in radish hypocotyls (1977)
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    Planta 136 (1977), S. 131-134 
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    ISSN: 1432-2048
    Keywords: Action-spectrum ; Blue-light photoreceptor ; High-irradiance-response ; Photomor-phogenesis ; Phytochrome ; Raphanus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In etiolated seedlings of Raphanus sativus L. the inhibition of hypocotyl elongation by continuous light showed a major bimodal peak of action in the red and far-red, and two minor peaks in the blue regions of the spectrum. It is argued that, under conditions of prolonged irradiation, phytochrome is the pigment controlling the inhibition of hypocotyl elongation by red and far-red light, but that its mode of action in far-red is different from that in red. A distinct pigment is postulated for blue light.
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    Regulation of enzyme levels by phytochrome in mustard cotyledons: Multiple mechanisms? (1977)
    Springer
    Planta 136 (1977), S. 181-186 
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    ISSN: 1432-2048
    Keywords: Enzyme Regulation ; Sinapis alba ; Phytochrome ; Phenylalanine ammonia-lyase ; Ribulosebisphosphate carboxylase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Phytochrome controls the appearance of many enzymes in the mustard (Sinapis alba L.) cotyledons. The problem has been whether the effect of phytochrome on the appearance of enzymes in this organ is due to a common initial action of Pfr, e.g. due to the liberation of a “second messenger”. We have compared the modulation by light (phytochrome) of the appearance of phenylalanine ammonia lyase (PAL)+ and ribulosebisphosphate carboxylase (Carboxylase)+. PAL becomes detectable in the mustard cotyledons at 27 h after sowing while Carboxylase starts to appear only at 42 h after sowing (starting points, 25° C). The starting points cannot be shifted by light. As a major result, in the case of PAL the inductive effect of continuous red light (given from the time of sowing) remains fully reversible by 756 nm-light up to the starting point (27 h after sowing) while with Carboxylase full reversibility in continuous red light is lost at approximately 15 h after sowing. While the induction of Carboxylase is already saturated at a very low level of Pfr (e.g. continuous 756 nm-light saturates the response) and does not depend on irradiance (e.g. continuous 675 mW m-2 red light and 67.5 mW m-2 red light lead to the same time course), PAL induction is a graded response over a wide range of Pfr doses and depends strongly on the fluence rate (high irradiance response, HIR). It is concluded that PAL induction and Carboxylase induction are not only separated in time but differ in every regard except that both responses are mediated by phytochrome. The present data support the previous conclusion that the specification of the temporal and spatial pattern of development is independent of phytochrome even though the realization of the pattern of development can only occur in the presence of phytochrome (Pfr). It seems that there is no feedback from pattern realization to pattern specification.
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    Phytochrome and potassium uptake by mung bean hypocotyl sections (1977)
    Springer
    Planta 137 (1977), S. 61-64 
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    ISSN: 1432-2048
    Keywords: Phaseolus ; Phytochrome ; Potassium uptake
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Uptake of potassium (K) and 86rubidiumlabelled potassium (86Rb) by sub-hypocotyl hook sections of Phaseolus aureus L. was inhibited by red light. The effect was reversible with far red light. Using short exposures of high irradiance the effect on 86Rb-labelled K uptake was observed after 5 min. The response showed no specificity for a particular anion. Uptake of 86Rb-labelled K by sections cut immediately below the cotyledons was enhanced by red light after 10 min incubation and was also far red reversible. These results are interpreted as a rapid phytochrome-induced change in membrane properties resulting in modified K uptake.
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    Phytochrome and calcium ions are involved in light-induced membrane depolarization in Nitella (1977)
    Springer
    Planta 137 (1977), S. 225-229 
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    ISSN: 1432-2048
    Keywords: Calcium Ions ; Membrane potential ; Nitella ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Isolated internodes of Nitella (N. opaca, N. flexilis) and Nitellopsis spec. were punctured with single microelectrodes and their membrane potentials were recorded continuously during various light treatments. In red light the initial response was always a depolarization. This depolarization began with a lag-time of 0.4-3.5s and reached a steady state within 1–2 min of continuous illumination. Repolarization began within several seconds after turning off the light. The magnitude of the red-light-induced depolarization increased with the Ca2+-concentration of the medium. The largest depolarizations were recorded in 5 m mol l-1 Ca2+. Ca2+ could not be replaced in this function by Na+, Mg2+, La3+ or mannitol. Far-red light alone had no effect on the resting membrane potential. Far-red light applied immediately after red light accelerated the repolarization of the membrane potential. Far-red light applied simultaneously with red light reduced the amount of depolarization and increased the rate of repolarization. The results indicate that phytochrome and Ca2+ are involved in the light-induced depolarization of the membrane. They are consistent with the hypothesis that phytochrome may act by triggering a Ca2+-influx at the plasma membrane.
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    Phytochrome modulation of ATPase activity in a membrane fraction from Phaseolus (1977)
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    Planta 137 (1977), S. 203-206 
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    ISSN: 1432-2048
    Keywords: ATPase ; Membrane ; Phaseolus ; Photomorphogenesis ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Membrane-bound phytochrome and ATPase (ATP phosphohydrolase EC 3.6.1.3.) activity extracted from hypocotyl hooks of etiolated Phaseolus aureus Roxb. were both separated from solute proteins by gel filtration on Sepharose C1-2B. The amount of phytochrome detected in the membrane fraction was very small and was not significantly increased by red irradiation (in vivo or in vitro). Membrane-bound ATPase activity was modulated in vitro by the phytochrome in the membrane fraction, being lower after red light than after far-red light. This effect was potentiated by a preliminary light reaction which occurred only in vivo and, in continuous red light, required 60 to 90 s at 25°C. Thus a two minute, in vivo, red irradiation reduced membrane-bound ATPase activity to about half that of the etiolated state. Subsequently bound-ATPase activity was determined by the form of phytochrome (Pr or Pfr) irrespective of whether established in vivo or in vitro. These results indicate that binding or release (of enzyme, cofactors or inhibitors) is not involved in phytochrome modulation of enzyme activity in the membrane fraction.
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    Nature of light requirement for the flowering of Chenopodium rubrum L. (Ecotype 60° 47′ N) (1977)
    Springer
    Planta 133 (1977), S. 103-106 
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    ISSN: 1432-2048
    Keywords: Chenopodium rubrum ; Flowering ; High Energy Reaction ; Photosynthesis ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Seedlings of C. rubrum were irradiated with different light qualities and intensities following a single inductive dark period. Our results show that relatively low intensity white light (35–100 ft. c.) does not support flower development while high intensity white light (650–800 ft. c.) permits 100% flowering. We have shown that the low intensity light inhibiton of flower development is not due to suboptimal photosynthesis. Relatively low intensities of light rich in far-red or blue wavebands sustains optimum flower development, whereas red light is totally ineffective in this respect. Considering that the intensity dependent High Energy Reaction (HER) has its action maxima in the blue and far-red we propose that HER may be positively involved in the flower development of C. rubrum. Our study further suggests that there may be some flower inhibitory component at play in relatively low intensity white light conditions and HER may be required to counteract this flower inhibitory effect.
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    Photocontrol of stem elongation in light-grown plants of Fuchsia hybrida (1977)
    Springer
    Planta 133 (1977), S. 149-156 
    Details
    ISSN: 1432-2048
    Keywords: Fuchsia ; Internode development ; Photoperiodism ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Stems of the caulescent long-day plant, Fuchsia hybrida cv Lord Byron, showed 2 types of response to light. In one, internode length was increased by far-red irradiation given at the end of an 8 h photoperiod: the response was no greater with prolonged exposure and was less when the start of far-red was delayed. The effect of far-red was reversible by a subsequent exposure to red light. Internode length was inversely proportional to the Pfr/P ratio established before entry to darkness and there was no evidence for loss of Pfr during a 16 h dark period. The inhibitory effect of Pfr acted at a relatively late stage of internode growth. With the development of successive internodes a second response appeared in which stems lengthened following prolonged daily exposures to red or far-red light, or mixtures of the two, or to brief breaks with red or white light. In these later internodes, a short exposure to far-red near the middle of the night was not reversible by red because red alone promoted elongation at this time. Internode length increased with increase in the daily duration of light and, when light was given throughout an otherwise dark period of 16 h, with increase in illuminance to a saturation value of 200 lx from tungsten lamps. Elongation increased as a linear function of decrease in photostationary state of phytochrome down to Pfr/P≃0.3; however, internodes were shorter in far-red light than in 25% red/red+far-red. It was concluded that stem length is a net response to two modes of phytochrome action. An inductive effect of Pfr inhibits a late stage in internode expansion, and a phytochrome reaction which operates only in light (and may involve pigment cycling) promotes an early stage of internode development. Stem elongation is thus a function both of the daily duration of light and its red/red+far-red content. The outgrowth of axillary buds was controlled by the first type of phytochrome action only.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00391913
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    Nature of light requirement for the flowering of Chenopodium rubrum L. (Ecotype 60° 47′ N) (1977)
    Springer
    Planta 133 (1977), S. 97-102 
    Details
    ISSN: 1432-2048
    Keywords: Chenopodium rubrum ; Flowering ; High Energy Reaction ; Photosynthesis ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Seedlings of the short-day plant, Chenopodium rubrum L. (Ecotype 60° 47′ N) were irradiated with different intensities and qualities of light for 24 h preceding a single inductive dark period (12 h). Our data shows that a relatively low intensity incandescent light (35–100 ft. c.) is not effective as the photoperiod for flowering. The above effect is not due to a requirement for a relatively high level of photosynthesis. Our results suggest a definite promotory role of a blue High Energy Reaction (HER). We could not demonstrate the involvement of a far-red HER. We suggest that ineffectiveness of far-red may have been due to establishment of rather low Phytochrome, P FR , levels, suboptimal for flowering. A certain critical level of P FR (30–40%, that presumably established by blue light) seems to be necessary for photoreactions involved in flowering of C. rubrum. There are indications in our experiments of the operation of a red radiation mediated flower inhibitory photoreaction.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00391904
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  • 89
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    The capacity of chlorophyll-a biosynthesis in the mustard seedling cotyledons as modulated by phytochrome and circadian rhythmicity (1977)
    Springer
    Planta 133 (1977), S. 295-302 
    Details
    ISSN: 1432-2048
    Keywords: 5-Aminolaevulinic acid ; Chlorophyll synthesis ; Circadian rhythm ; Phytochrome ; Sinapis alba L.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Within the temporal pattern of “primary differentiation” the capacity of chlorophyll — a biosynthesis in the cotyledons ofSinapis alba L. seedlings is controlled by phytochrome (in continuous light) or by releasing the circadian rhythm either with lightdark cycles or by a light→dark transition. The sensor pigment for this process is phytochrome. It is very probable that in continuous light as well as under conditions under which the circadian rhythm plays the major part, the capacity of chlorophyll a biosynthesis is limited by the capacity of the biosynthetic step which produces 5-aminolaevulinate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00380692
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  • 90
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    Light-induced changes in the photoresponses of plant stems the loss of a high irradiance response to far-red light (1977)
    Springer
    Planta 135 (1977), S. 95-100 
    Details
    ISSN: 1432-2048
    Keywords: De-etiolation ; High-irradiance response ; Photomorphogenesis ; Phytochrome ; Raphanus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract De-etiolation results in phytochrome destruction, greening, and the loss of the far-red high irradiance responses (HIR). Evidence is presented against the hypothesis that the loss of the far-red HIR is a direct consequence of phytochrome destruction. Loss of the far-red HIR for the inhibition of elongation in hypocotyls of Raphanus sativus involves two different, but linked, actions of phytochrome. An induction reaction requires the far-red absorbing form of phytochrome for about 20 min after which accumulation of its product depends only on time. A second reaction requires continuous light or frequent short irradiations and involves cycling of the phytochrome system. This acts on the product of the induction reaction. It is proposed that in green plants an important mode of operation of phytochrome in the light depends on pigment cycling, and that during de-etiolation this system is established under phytochrome control.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00387156
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  • 91
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    The loss of phytochrome photoreversibility in vitro (1977)
    Springer
    Planta 135 (1977), S. 217-223 
    Details
    ISSN: 1432-2048
    Keywords: Killer ; Phytochrome ; Pisum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract “Killer”, a substance extracted from stem tissue of etiolated pea seedlings (Pisum sativum L. v. Alaska), interacts specifically with the far-red-absorbing form of phytochrome (Pfr) in vitro in a temperature-independent, rapid, stoichiometric fashion to cause a loss of phytochrome photoreversibility. The chromatographic, solubility, and spectral properties of partially purified fractions indicate that Killer is a cyclic, unsaturated molecule containing ionizible hydroxyl groups; its molecular weight is unknown, although probably low. Possible mechanisms by which the Killer-phytochrome interaction results in the loss of photoreversibility are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00384893
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  • 92
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    The role of cotyledons in phototropism of de-etiolated seedlings (1977)
    Springer
    Planta 135 (1977), S. 51-55 
    Details
    ISSN: 1432-2048
    Keywords: Blue light ; Cotyledons ; Cucumis ; Helianthus ; Phototropism ; Phytochrome
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Simulated “phototropic” curvatures caused by differential masking of the cotyledons of de-etiolated seedlings exposed to white light are unconnected with true phototropism. In Cucumis sativus L. and Helianthus annuus L. such curvatures result from a red-light-induced inhibition coming from the exposed cotyledon. True phototropic bending in these species under long-term exposure to fairly high irradiances (as in nature) is a response to blue light. It occurs even when cotyledons are completely covered. These results show that the cotyledons do not perceive the phototropic stimulus and need not be illuminated for phototropism to occur.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00387975
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  • 93
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    Identification of LSD and ergot alkaloids by thin-layer chromatography (1977)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 283 (1977), S. 32-32 
    Details
    ISSN: 1618-2650
    Keywords: Nachw. von LSD, Ergot-Alkaloiden ; Chromatographie, Dünnschicht
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00439765
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    Thin-layer chromatography of some ergot alkaloids (1977)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 284 (1977), S. 47-48 
    Details
    ISSN: 1618-2650
    Keywords: Analyse von Ergotalkaloiden ; Chromatographie, Dünnschicht
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00464041
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    Detection and determination of dichlorovos (DDVP) in toxicological analysis (1977)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 285 (1977), S. 48-48 
    Details
    ISSN: 1618-2650
    Keywords: Nachw., Best. von Dichlorvos in Biolog. Material ; Chromatographie, Dünnschicht
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00446021
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    Thin-layer chromatographic separation of some isomeric thiazolylbenzothiazoles (1977)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 285 (1977), S. 255-255 
    Details
    ISSN: 1618-2650
    Keywords: Trenn. von Thiazolylbenzothiazolen ; Chromatographie, Dünnschicht ; isomere
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00453575
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  • 97
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    Über chromatographische Trennungen der Seltenen Erden (1977)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 286 (1977), S. 50-53 
    Details
    ISSN: 1618-2650
    Keywords: Trenn, von Seltenen Erden ; Chromatographie, Dünnschicht ; Verteilungsverfahren ; Tetrahydrofuran, Triphenylphosphinoxid, Paraldehyd, Di-iso-propyläther-Tetrahydrofuran
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Die Verteilungskonstanten von Selten-Erd-Nitraten im System Tributylphosphat-wäßrige Nitrat-Lösung werden berechnet. Dünnschicht-chromatographisch können mit Tributylphosphat in Isooctan als Laufmittel die Selten-Erd-Nitrate von La bis Gd vom Gd bis Lu getrennt werden. Eine Trennung von 13 Seltenen Erden auf einem Chromatogramm gelingt mit den Laufmitteln a) Tetrahydrofuran/Paraldehyd/Salpetersäure (20∶70∶10), b) dem Laufmittel Triphenylphosphinoxid (0,3 M in Paraldehyd) und konz. Salpetersäure im Verhältnis 90∶10, c) Di-iso-propyläther/Tetrahydrofuran/Salpetersäure (100 ∶ 60 ∶ 10). Der Trennmechanismus wird diskutiert.
    Notes: Abstract The distribution constants of the rare earth nitrates in the system tri-n-butylphosphate/aqueous nitrate solution are calculated. With thin-layer chromatography it is possible to separate the rare earth nitrates from La to Gd and from Gd to Lu in the solvent system tri-n-butylphosphate in i-octane. A separation of 13 rare earths in only one chromatogram is possible in the solvent systems a) tetrahydrofuran/paraldehyde/nitric acid (20∶70∶10), b) tri-phenylphosphinoxide (0.3 M in paraldehyde)/conc. nitric acid in the ratio 90∶10, c) di-iso-propylether/tetrahydrofuran/nitric acid (100∶60∶10). The mechanism of the separation is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00552348
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    Steuergerät zur automatischen spektralphotometrischen Direktauswertung von Dünnschicht-Chromatogrammen (1977)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 285 (1977), S. 111-120 
    Details
    ISSN: 1618-2650
    Keywords: Chromatographie, Dünnschicht ; Steuergerät zur automatischen Registrierung
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Ein Gerät zur vollautomatischen Steuerung eines DC-Spektralphotometers wurde entwickelt. Zusätzlich zur automatischen Registrierung aller Spuren eines Dünnschicht-Chromatogramms werden Remissionsminima erkannt und ihre Remissionsgrade und Ortskoordinaten ausgedruckt. Eine zweite Betriebsart ermöglicht ein manuelles Abrastern und Ausmessen eines Fleckes an jeder beliebigen Stelle der DC-Platte. Ein Rechneranschluß zur Auswertung der Meßergebnisse ist vorhanden. An Hand von Beispielen wird gezeigt, daß Reproduzierbarkeits- und Konzentrationsfehler bei der automatischen Registrierung strichförmiger Flecke wesentlich kleiner sind als bei Handpositionierung kreisförmiger Flecke. Die Linearität verschiedener Analysenfunktionen wird über 2 Zehnerpotenzen der Strichbelegung untersucht. Dabei erweist sich die Kubelka-Munk-Funktion in bezug auf Linearität allen anderen untersuchten Funktionen überlegen. Eine Kosten-Nutzen-Rechnung zeigt, daß in einem Routinelabor durch Einsatz eines Auftragegerätes und eines Steuergerätes für die automatische Registrierung trotz höherer Investitionskosten die Gesamtkosten einer Analyse um 70% gesenkt werden können.
    Notes: Abstract An instrument has been constructed for the automatic control of a TLC spectrophotometer. In addition to the automatic scanning of all tracks of a thin-layer chromatogram the positions and values of diffuse reflectance minima are recognized and printed. A second mode allows the manual scanning and measurement of a spot at any position on the TLC plate. A digital output is provided for the evaluation of the results with a computer. The errors of reproducibility and of concentration are much smaller using the automatic scanning of bands than using the manual positioning of circular spots as the examples show. The linearity of several analytical functions is analysed within two decades of concentration. The Kubelka-Munk function was found to be superior in linearity to all other functions studied. Calculations of costs show, that the total costs of an analysis in a routine laboratory can be lowered to an extend of about 70% by the use of an application instrument and a controlling instrument for automatic scanning inspite of the higher costs of investments.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00573709
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    TLC Separation of potential antifertility agents: N-aryl-N′-2(p-methoxybenzoylbenzofuranyl)thiosemicarbazones (1977)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 285 (1977), S. 270-270 
    Details
    ISSN: 1618-2650
    Keywords: Trenn. von Aryl-p-methoxybenzoylbenzofuranyl-thiosemicarbazonen, Contraceptiva ; Chromatographie, Dünnschicht
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00453591
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    Dünnschicht-chromatographischer Nachweis herbicider Verbindungen durch Dansylierung (1977)
    Springer
    Fresenius' Zeitschrift für analytische Chemie 286 (1977), S. 95-99 
    Details
    ISSN: 1618-2650
    Keywords: Nachw. von Herbiciden ; Chromatographie, Dünnschicht ; Dansylierung
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Die Reaktion von Dansylchlorid mit verschiedenen Herbiciden und Derivaten, die Aminogruppen enthalten oder zu Anilinen hydrolysierbar sind, wird beschrieben. Auf der Dünnschichtplatte lassen sich die fluorescierenden Stoffe gut trennen und identifizieren, was anhand von schematischen Darstellungen der Dünnschicht-Chromatogramme gezeigt wird.
    Notes: Abstract The reaction of dansyl chloride with various herbicides and derivatives containing amino groups or hydrolyzable to anilines is described. On TLC plates the fluorescent substances can be readily separated and identified as shown by diagrammatic representation of the thin-layer chromatograms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00552357
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