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  • Articles  (10,476)
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  • Articles  (10,476)
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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Environmental management 8 (1984), S. 309-324 
    ISSN: 1432-1009
    Keywords: Animals ; Indicators ; Air pollution ; Ecosystem responses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract With existing and proposed air-quality regulations, ecological disasters resulting from air emissions such as those observed at Copperhill, Tennessee, and Sudbury, Ontario, are unlikely. Current air-quality standards, however, may not protect ecosystems from subacute and chronic exposure to air emissions. The encouragement of the use of coal for energy production and the development of the fossil-fuel industries, including oil shales, tar sands, and coal liquification, point to an increase and spread of fossil-fuel emissions and the potential to influence a number of natural ecosystems. This paper reviews the reported responses of ecosystems to air-borne pollutants and discusses the use of animals as indicators of ecosystem responses to these pollutants. Animal species and populations can act as important indicators of biotic and abiotic responses of aquatic and terrestrial ecosystems. These responses can indicate long-term trends in ecosystem health and productivity, chemical cycling, genetics, and regulation. For short-term trends, fish and wildlife also serve as monitors of changes in community structure, signaling food-web contamination, as well as providing a measure of ecosystem vitality. Information is presented to show not only the importance of animals as indicators of ecosystem responses to air-quality degradation, but also their value as air-pollution indices, that is, as air-quality-related values (AQRV), required in current air-pollution regulation.
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  • 2
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    Archives of microbiology 138 (1984), S. 273-277 
    ISSN: 1432-072X
    Keywords: Cyanobacteria ; Secondary metabolite ; Allelopathy ; Photosynthesis ; Electron transport ; Thylakoids ; Herbicides ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cyanobacterin is a secondary metabolite produced by the cyanobacterium, Scytonema hofmanni. Highly purified cyanobacterin was found to inhibit the growth of many cyanobacteria at a minimum effective dose of 2 μg/ml (4.6 μM). The antibiotic had no effect on eubacteria including the photosynthetic Rhodospirillum rubrum. The site of action of cyanobacterin was further investigated in the unicellular cyanobacterium, Synechococcus sp. Electron micrographs of antibiotic-treated Synechococcus cells indicated that cyanobacterin affects thylakoid membrane structure. The antibiotic also inhibited light-dependent oxygen evolution in Synechococcus cells and in spheroplasts. These data support our conclusion that cyanobacterin specifically inhibits photosynthetic electron transport. This activity is similar to herbicides such as 3-(3,4-dichlorophenyl)-1,1-dimethyl urea (DCMU). The anhydro analog of cyanobacterin had no biological activity.
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  • 3
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    Archives of microbiology 140 (1984), S. 265-270 
    ISSN: 1432-072X
    Keywords: EcoRI ; EcoRI-DNA complexes ; EcoRI* activity ; Recognition sites ; Frequency of binding ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Electron microscopy of negatively stained isolated restriction enzyme EcoRI revealed particle projections with triangular or square outlines, indicating that the enzyme, in its tetrameric state, is tetrahedron-like. The two dimers making up the tetramer appear to be arranged in two planes orthogonal to each other. Complexes formed by EcoRI with the plasmids pBR322 or pGW10 were investigated by electron microscopic spreading techniques. In the presence of Mg2+, EcoRI was bound to the DNA molecules to form pearl necklace-like aggregates. The number of bound EcoRI particles was much higher as the sum of EcoRI-and 5′..AATT..3′ sites (with exceptions, the 5′..AATT..3′ sites may function as one type of EcoRI* sites) along the DNAs, indicating unspecific binding. In the absence of Mg2+, EcoRI was bound to the DNA only at the recognition site for EcoRI and the sites where the tetranucleotide sequence 5′..AATT..3′ was present. A direct correlation of the local concentrations of the bases A and T within the flanking sequences of the binding sites with the frequency of EcoRI to the DNA was observed. Dimers and tetramers of the enzyme was found to bind to the DNA. Tetramers occasionally exhibited two binding sites for DNA as indicated by the observation of DNA loops originating at the sites of bound tetrameric EcoRI particles.
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  • 4
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    Cell & tissue research 237 (1984), S. 219-226 
    ISSN: 1432-0878
    Keywords: Spermatogonia ; Spermatocytes ; Carbohydrates ; Guppy ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The structure of guppy (Poecilia reticulata) spermatogonia and spermatocytes has been studied using electron microscopy. The spermatogonia, situated at the apex of the seminiferous tubule, are almost all surrounded by a network of Sertoli cells; they have very diffuse chromatin and one or two large nucleoli. The cytoplasm contains relatively few organelles, although annulate lamellae are found. The mitochondria have few cristae and are concentrated at one pole of the cell; they are sometimes found with intermitochondrial cement. These spermatogonia are separated from each other, having no intercellular bridges or inclusion in Sertoli cells, and are relatively undifferentiated; they correspond to stem cells. The spermatogonia beneath the apex are organized into cysts. First-generation spermatogonia are more dense and heterogeneous, their nuclei becoming smaller and their chromatin becoming denser during successive generations. In spermatocytes, the synaptinemal complex exists as a modified form until metaphase. The concentration of organelles in the cytoplasm increases and the organelles become more diversified as spermatogenesis progresses. Many cytoplasmic bridges are observed (several per cell), indicating that the cells remain in contact after several divisions. These changes in germ cell structure have been related to some of the characteristic features of spermatogenesis in guppy, e.g. the large number of spermatogonial generations and the complexity of spermiogenesis.
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  • 5
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    Cell & tissue research 238 (1984), S. 413-416 
    ISSN: 1432-0878
    Keywords: Peroxisomes ; DAB-cytochemistry ; Electron microscopy ; Liver, amphibian ; Gymnophiona ; Ichthyophis glutinosus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure of hepatic peroxisomes was investigated in Ichthyophis glutinosus (Amphibia: Gymnophiona), employing perfusion fixation and the diaminobenzidine (DAB) technique for the visualization of catalase. The majority of peroxisomes is circular or rod-shaped, although elongated particles occasionally occur. They contain a finely granular matrix, lightly stained after the DAB procedure. Their mean diameter is approximately 0.25 μm. Serial sections reveal that the circular and rod-shaped peroxisomal profiles are cross and oblique sections of highly tortuous, tubular organelles exceeding 2 μm in length. In addition to tubular profiles, elongated, rectangular particles, as well as straight dumbbell-shaped organelles with distinct marginal plates are observed. They range from 900 to 1650 nm in length (mean = 1200 nm). In the flattened, thin central portion of the dumbbell-shaped particle, the peroxisomal membranes form a cisterna enclosing one or two uniformly thick marginal plates, which display a definite substructure with a periodicity of 10 nm. These findings indicate that peroxisomes in the liver of Ichthyophis exhibit a complex organization. It is suggested that the organelles undergo a specific differentiation process, morphologically characterized by the formation of enlarged segments of unusual shape.
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  • 6
    ISSN: 1432-0878
    Keywords: Ovulation (rabbit) ; Graafian follicle ; Perfusion ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Isolated ovaries from untreated, sexually mature rabbits were introduced into an in vitro perfusion system and perfused with a chemically defined medium containing albumin. The ovaries were perfused for up to 15 h (mean 11.5 h) and then processed for morphological investigation. Both at the light- and electron-microscopical levels, most of the ovaries exhibited a normal structure comparable with ovaries in situ. In two cases, however, marked accumulations of bacteria were found, although not inside the follicles. Since ovulation in the rabbit normally occurs between 9.5–13 h after mating or human chorionic gonadotrophin treatment, this model seems adequate for studies of ovulation in vitro. It is, however, important to study the ovaries microscopically after the perfusion to detect artifacts, e.g., bacterial infection, that may have influence on the process of ovulation.
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  • 7
    ISSN: 1432-0878
    Keywords: Tannic acid ; Acetylcholine receptors ; Tissue culture ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Spinal cord neurons from 9-day chick embryos were maintained in culture for up to 35 days and then fixed in 4% cacodylate-buffered glutaraldehyde containing 2% tannic acid. After about 15 days in culture a small percentage of the synaptic specializations present were characterized by striking electron-dense striations averaging 15 nm in width, oriented perpendicular to the postsynaptic membrane. These structures increased in frequency with time in culture (to a maximum of about 10% of all synapses in the oldest cultures); they were asymmetrical, protruding approximately 8 nm into the synaptic cleft, and more deeply (approximately 15–18 nm), into the postsynaptic cytoplasm. On the basis of earlier work by Sealock (1980) they are interpreted as concentrations of acetylcholine receptors. Similar membrane differentiations were also seen associated with active-zone areas of a few presynaptic membranes, and the possibility that these represent presynaptic acetylcholine receptors is discussed. Additional observations reported are (1) the presence of striations resembling those seen at the postsynaptic membrane in the membranes of some postsynaptic vesicles, and (2) filamentous links between the striations and cytoskeletal elements of the postsynaptic cell.
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  • 8
    ISSN: 1432-0878
    Keywords: Monoamine-containing cells ; Taste bud ; Paracrine cells ; Mechanoreceptors ; Electron microscopy ; Teleosts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The taste buds on the barbels in three species of teleosts (Cyprinus carpio, Misgurnus anguillicaudatus, Parasilurus asotus) were studied by means of fluorescence and electron microscopy. Intensely yellow-fluorescent cells, which are disk-shaped and located exclusively in a basal position, are observed in the barbel-buds of all fishes examined. The basal cells contain a large number of small clear vesicles approximately 40–60 nm in diameter, which show a tendency to aggregate in the cytoplasm facing the junction of the nerve terminals; chemically transmitting synapses are seen in the latter region. It is suggested from the present observations that the basal cells in the barbel-bud may originate from Schwann cells and have a dual function both as mechanoreceptors and paracrine elements. Since the administration of 5,6-DHT results in an appearance of small dense vesicles among the small clear vesicles, the possibility exists that the basal cell may be capable of taking up monoamines and storing them in the small clear vesicles.
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  • 9
    ISSN: 1432-0878
    Keywords: Exocrine pancreas ; Calcium pool ; Calcium release ; Electron microscopy ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In an attempt to identify a cellular Ca2+-pool, from which calcium is released when secretagogues are applied, tissue fragments of the rat exocrine pancreas were incubated and fixed with glutaraldehyde in the presence of calcium. By means of this procedure electron-dense deposits were found on plasma membranes. X-ray microanalysis showed that these deposits contain calcium. Stimulation of tissue fragments with the use of the secretagogues carbachol or cholecystokinin reduced the number of deposits by about 80%. When the antagonist atropine was applied after carbachol stimulation, deposits reappeared on cell membranes, which then disappeared again after a second stimulation with cholecystokinin. In the presence of procaine, carbachol was inhibited and only slightly reduced the Ca2+-deposits on the plasma membranes. These results suggest that a calcium pool, from which calcium is released to induce enzyme secretion on stimulation, is located in the cell membrane
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  • 10
    ISSN: 1432-0878
    Keywords: Chloride cells ; Acid stress ; Gill ; Electron microscopy ; Fathead minnow
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Fathead minnows, Pimephales promelas, were exposed for 129 days to Lake Superior water acidified with sulfuric acid by means of a flow-through toxicant injection system. The effects of chronic acid stress (pH 6.5, 6.0, 5.5, 5.0) on gill histology were examined. Most of the histological effects were seen at pH 5.5 and 5.0 and were confined primarily to changes in numbers, distribution, and morphology of chloride cells. At low pH levels there tend to be more chloride cells in the gill epithelium and an increased percentage of these cells in the secondary lamellae. In contrast to normal chloride cells, chloride cells from fish exposed to low pH frequently had apical pits while some had bulbous apical evaginations. The occurrence of structural changes in chloride cells during exposure to acid water suggests that chloride cells may be involved in acclimation to acid stress.
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  • 11
    ISSN: 1432-0878
    Keywords: Lactating cell ; Lipid droplets ; Secretory vesicles ; Mitochondria ; Intracellular associations ; Electron microscopy ; Milk secretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The size, cellular location, and identity of surface-associated components were determined for lipid droplets in lactating cells. Transmission electron-microscopic measurements were made involving 3801 droplets in approximately 211 cells from three rats and 1197 droplets in 66 cells from a mouse. For the purposes of droplet evaluation, cells were divided into seven locations ranging from basal to secreting positions. Droplets were also categorized with respect to contact with other droplets, basolateral plasma membrane, mitochondria, Golgi apparatus, secretory vesicles, and endoplasmic reticulum-cytoplasm (ERC). Data on droplet size showed that droplet growth occurs mainly in the secretory position, confirming previously published findings. Lipid droplets from mouse tissue, although somewhat smaller in size showed similar growth trends to those of the rat. Data on numbers of droplet contacts and percentages of droplet circumferences involved in associations with other cell components showed that the dominant interaction of lipid droplets was with the ERC. However, intimate association of droplets with mitochondria was noted in all cellular locations. In addition, nursed animals exhibited a greater proportion of droplet surface association with secretory vesicles and less in contact with mitochondria in comparison to those not nursed. The significance of these relationships to milk synthesis and secretion is discussed.
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  • 12
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    Cell & tissue research 236 (1984), S. 249-255 
    ISSN: 1432-0878
    Keywords: Oocyte ; Nucleolus ; Silver staining ; Electron microscopy ; Rat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The nucleoli of dictyate-stage growing oocytes in rat ovaries were examined both with routine electron microscopy and electron microscopy after silver nitrate and ammoniacal silver nitrate (Ag-AS) staining. The nucleoli of the unilaminar follicular oocytes consist of twisted strands of dense fibrillar components, aggregates of granular components, and small fibrillar centers. After Ag-AS staining, silver grains are numerous on the dense fibrillar strands, fewer on the fibrillar centers, and very sporadic on the granular aggregates. The same stainability of three nucleolar components with the Ag-AS method was also confirmed in the nucleoli segregated by actinomycin D. During the transition of growing oocytes from bilaminar to plurilaminar follicle stage, the nucleolar dense fibrillar strands gradually conglomerate and are transformed into large and compact spherules. The stainability of dense fibrillar components with the Ag-AS method was lost along with this nucleolar transformation. These results may provide some new clues on the functional significance of AgAS-positive proteins in the nucleoli.
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  • 13
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    Cell & tissue research 235 (1984), S. 51-58 
    ISSN: 1432-0878
    Keywords: Parathyroid glands ; Electron microscopy ; Light microscopy ; Quantitative histology ; Mongolian gerbil
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Stereology and semi-automatic image analysis were used with the aim of comparing the structure of parathyroid glands from untreated adult Mongolian gerbils fixed by immersion with those fixed by perfusion. Subclassification of the chief cells based upon the staining affinity or electron density of the cytoplasm was readily performed only in glands fixed by immersion, and so-called atrophic cells were observed only in these glands. The atrophic cells were often surrounded by “light” chief cells. In glands fixed by perfusion, “light” chief cells were only rarely encountered. A significant difference between glands fixed by immersion and those fixed by perfusion was found only with regard to the form of cells and nuclei, those fixed by perfusion being more spherical. When comparing individual cells within glands fixed by immersion, “light” chief cells were more spherical and had a significantly larger nuclear and cellular size, and a lower mitochondrial volume density than the “intermediate”/“dark” chief cells. Otherwise there were no significant differences in any of the parameters investigated. These data indicate that occurrence of socalled “light” chief cells and atrophic cells is a result of improper fixation. The results of this study do not favour the concept of a functional cycle with a simultaneous occurrence of active and inactive cells within parathyroid glands.
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  • 14
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    Cell & tissue research 235 (1984), S. 153-158 
    ISSN: 1432-0878
    Keywords: Bovine ; Interdigitating cell ; Lymphoid tissues ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Electron-microscopic studies of lymphoid tissues from bovine fetuses and from calves disclosed a non-lymphoid cell type in the thymus-dependent zones of secondary lymphoid tissues and in the thymus that is distinguishable from reticulum cells, epithelial and endothelial cells, and macrophages. Based on morphological and topographical criteria, the cell is identified as the interdigitating cell. In addition, studies of the tissues of normal and virus-challenged fetuses, and of conventionally reared calves, indicated that the interdigitating cells originate from monocytoid cells, which undergo differentiation in the thymus-dependent zones during an immune response.
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  • 15
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    Cell & tissue research 235 (1984), S. 243-249 
    ISSN: 1432-0878
    Keywords: Gap junction ; Electron microscopy ; Freeze fracture ; Cell-to-cell communication
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Gap junctions exist in the septa between the segments of the lateral giant axons in the ventral nerve cord of the crayfish Procambarus. A large increase in the resistance (uncoupling) of these gap junctions was brought about by mechanical injury to the axonal segments. Both thin sections and freeze-fracture preparations were used to monitor the morphological changes which occurred up to 45 min after injury. There was no apparent change in the organization (a loose polygonal array) of the intramembrane particles which make up the junctional complex up to 45 min after injury. In some instances, however, the intramembrane particles appeared to have moved away from the junctional area. Other junctional regions were internalized and appeared similar to what have been called annular gap junctions. Also at this time (20–25 min after injury), a dense cytoplasmic plug formed in uninjured axon near the junctional region. It is concluded that the gap junctions that exhibit a loose polygonal organization of the intramembrane particles may be either in a state of low resistance (coupled) or a state of high resistance (uncoupled).
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  • 16
    ISSN: 1432-0878
    Keywords: Ovulation ; Perfusion ; Graafian follicle (rabbit) ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Ovulation was induced in rabbits by intravenous administration of human chorionic gonadotrophin (HCG), and 4–5 h later the ovaries were isolated and introduced into an in-vitro perfusion system containing synthetic medium with albumin. Rupture of follicles occurred in vitro within the physiological time range (mean 11.3 h after injection of HCG), although with a reduced frequency. Preovulatory and ruptured follicles were studied in detail by light and electron microscopy. In the granulosa layer of ruptured or preovulatory follicles cytoplasmic blebbing activity, disappearance of CallExner bodies and differentiation toward luteinized cells were found. Perhaps the most important sign of normal preovulatory development in vitro was that the basement membrane surrounding the granulosa layer was penetrated by projections of granulosa cells. In the absence of this penetration phenomenon the granulosa layer prolapsed out of the follicle. Immediately before rupture, follicles showed marked degeneration, restricted to the outer layers of the apical wall, which is compatible with the hypothesis that degradative enzymes are released close to the surface of preovulatory follicles. Although the majority of follicles that ovulated under in-vitro conditions showed the same kind of morphological alterations as can be seen in vivo, occasional atypical ruptures occurred without any overt signs during perfusion. Also technical manipulations of the perfusion system, e.g., nonphysiological increase of perfusion pressure, could force follicles to rupture. This illustrates the importance of careful morphological study of all ovaries perfused in vitro before conclusions are drawn.
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  • 17
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    Cell & tissue research 237 (1984), S. 149-154 
    ISSN: 1432-0878
    Keywords: Pituitary gland, pars distalis ; Innervation ; Synaptoid contacts ; Electron microscopy ; Rana temporaria L
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary In the pars distalis of the hypophysis of adult Rana temporaria, three types of nerve-fiber profiles were found at two distinct sites, in both lateral parts of the bordering regions of the anterior lobe with the intermediate lobe of the hypophysis. The first type of nerve-fiber profile consists of bundles of very fine axonal elements (diameter: 〈0.7 μm). The second type is formed by larger nerve fibers (diameter up to 4 μm) containing a few neurosecretory granules of approximately 100 nm. The third type of nervefiber profile resembles the second type but these nerve fibers make synaptoid contacts on at least two different types of glandular cells. The possible functional significance of these nerve fibers in the pars distalis is discussed. No nerve fibers were found (1) in the central part of the bordering region of the pars distalis with the intermediate lobe, (2) at the bordering region with the median eminence and (3) with the neurohypophysial stalk, and (4) in all other parts of the pars distalis.
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  • 18
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    Cell & tissue research 238 (1984), S. 165-175 
    ISSN: 1432-0878
    Keywords: Mesonephros ; Pig embryo ; Glomerulus ; Microvasculature ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Vascularization of the pig mesonephros was investigated in embryos 5–8 cm in length. Vascular injections with microfil were cleared and dissected; corrosion casts were studied under the scanning electron microscope (SEM). Perfusion-fixed tissue was used for SEM and transmission electron microscope (TEM) studies, including freeze-fracture specimens. The branches of one mesonephric artery carry up to 15 glomeruli. Several glomeruli occupy the same arterial branch, with very short afferent arterioles proper. The efferent vessels, frequently 2–5, leave the extensive vascular pole opposite the entering arteriole and split into peritubular capillaries radiating towards the superficial veins. These capillaries form vascular regions in the shape of flattened pyramids. Along its course, one nephron is supplied by vessels derived from 4–7 glomeruli. The nephrons have less vascular contact than in the definitive kidney. The ultrastructure of the single mesonephric vessels matches the metanephric counterparts. Epithelioid cells with renin granules are common in afferent arterioles, larger arteries, and efferent vessels. The lobulated glomeruli are up to 750 μm long and flattened, showing the usual features of podocytes, mesangial cells, and an attenuated endothelium with fenestrations between 50 and 250 μm. It partially retains its own basement membrane. There is no proximal mesangium.
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  • 19
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    Cell & tissue research 238 (1984), S. 387-394 
    ISSN: 1432-0878
    Keywords: Pacinian corpuscles, cat ; Denervation ; Atrophy ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary The ultrastructure of Pacinian corpuscles of the cat located in the crural region and innervated by the interosseous nerve was studied 1 to 14 months after denervation. Both the Pacinian inner core and capsule remained well preserved one month after denervation. However, the denervated inner cores underwent progressive atrophy and wasting, which resulted in a gradual reduction of the amount of inner-core cells and lamellae, widening of interlamellar clefts, formation of empty spaces in the axial region and a considerable increase in the number of collagen fibrils. In spite of the wasting, the inner core still survived 14 months after denervation, but at least half of its volume became occupied by collagen fibrils which surrounded the remaining inner-core cells and lamellae. Collagen fibrils assembled in the denervated core were markedly thinner than those found in the capsule, as is also the case in normal Pacinian corpuscles. In the capsule, discrete focal degeneration, occasional pyknosis of the innermost capsular cells and macrophage infiltration were observed from the first month after nerve section onward, but the number of capsular layers remained within the normal range (30–40) up to 14 months after denervation.
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  • 20
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    Protoplasma 119 (1984), S. 48-54 
    ISSN: 1615-6102
    Keywords: Electron microscopy ; Marine alga ; Membrane contrast ; Osmium-azole complexes ; X-ray microanalysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Addition of certain heterocyclic nitrogen-carbon compounds to standard osmium tetroxide solutions used as secondary fixative resulted in an enhanced general membrane contrast in cells of the marine algaEmiliania huxleyi (Lohmann) Kamptner. Ultrastructural cell morphology and the contrast distribution were compared between cells treated according to a standard secondary fixation procedure and cells post-fixed when above mentioned heterocyclic compounds were introduced; in both cases some of the ultrathin sections were post-stained. Different compounds were tested: 1,2,4-triazole (TRA), 3-amino-1,2,4-triazole (A-TRA), 5-amino-tetrazole (A-TEA) and 2,4,6-tri-amino-1,3,5-triazine (melamine). The results were interpreted to indicate the possible bonding types arising from interaction of the heterocyclic compounds with osmium tetroxide and with membrane constituents. Interpretations were partly inspired by considerations from coordination chemistry. All above tests which did not include post-staining of thin sections could be performed at alkaline pH, and consequently calcified structures were preserved. The enhanced osmium accumulation at membranes was verified with X-ray microanalysis, which also showed that in the cases where membranes were visibly contrasted, localization of probable sites of intracellular non-crystalline calcium was facilitated.
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  • 21
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    Plant and soil 76 (1984), S. 127-137 
    ISSN: 1573-5036
    Keywords: Adenylate pool ; Biomass volume ; CO2 evolution ; Chitin ; DNA ; Electron microscopy ; Enzymes ; Fluorescent antibody ; Fumigation-respiration ; Fungi Histochemistry ; Imunofluorecence ; Jones-Mollison technique ; Microcosms ; Monoclonal antibodies ; Nitrogen ; Nutrients ; Oxygen consumption ; Phosphorus ; Phytotoxins ; Plate counts ; Rhizobium ; Rhizosphere ; Sulphur ; Xenobiotics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary There is an immense literature on biological and biochemical analyses of soils. Such analyses have revealed the enormous richness of species in soil and their vast range of metabolic potentials and ecological diversity. Accordingly, the approaches used to investigate the soil biota and its biochemistry usually have to be modified or adapted depending upon the purpose of the investigation. Studies of micro-organisms in the soil environment, are complicated because microbial cells are commonly attached to surfaces where they live side-by-side with other populations in consortia usually containing different morphological and physiological types. Such assemblages of organisms cannot be described quantitatively using cultural techniques, such as plate counts, which underestimate both cell numbers and viable biomass. The development of more powerful observational and staining techniques has improved our knowledge of the diverse morphological and biochemical composition of soil micro-communities. Such findings have been amplified at a grosser level by laboratory studies with multi-component systems (microcosms) to mimic field situations and to assess the range of biochemical potentials of microbial consortia. But despite notable advances in analytical methods we are still, with a few exceptions, unable to detect or identify those microorganisms which carry out specific biochemical transformations or determine whether particular cells are alive, dormant or dead at the time of observation. Considerable work has been done to define some of the fundamental ecological attributes of microbial assemblages in soil. Productive work on the metabolic activities of the soil microbiota, specially geochemical transformations of C, N, S and P, has been under way for more than a century. But only in more recent years have more sensitive and reproducible analytical methods become available to measure viable biomass in soil. This will enable some insight to be gained into the role that microbial biomass plays as a labile source and sink for plant nutrients.
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  • 22
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    Plant and soil 76 (1984), S. 319-337 
    ISSN: 1573-5036
    Keywords: Aggregates ; Aluminium ; Bacterial mucilage ; Binding agents ; Calcium ; Cation bridges ; Complexing agents ; Dispersion ; Electron microscopy ; Electrophoretic mobility ; Fungal hyphae ; Glues Iron ; Management Periodate ; Polysaccharides ; Rhizosphere ; Roots ; Slaking
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The stability of pores and particles is essential for optimum growth of plants. Two categories of aggregates macro- (〉 250 μm) and micro- (〈250 μm) depend on organic matter for stability against disruptive forces caused by rapid wetting. Dispersion of clay particles from microaggregates is promoted by adsorption of complexing organic acids which increase the negative charge on clays. The acids are produced by plants, bacteria and fungi. However, the dispersibility of clay in microaggregates is offset by the binding action of polysaccharides, mainly mucilages produced by bacteria, but also by plant roots and fungal hyphae. The stability of microaggregates is also enhanced by multivalent cations which act as bridges between organic colloids and clays. Macroaggregates are enmeshed by plant roots, both living and decomposing, and are thus sensitive to management, and increase in number when grasses are grown and the soil is not disturbed. Lack of root growth,i.e. fallow, has the opposite effect. Various implications for management of soil structure are discussed.
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    International Journal for Numerical Methods in Engineering 20 (1984) 
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    Keywords: Engineering ; Engineering General
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 1-14 
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    Keywords: Engineering ; Engineering General
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    Notes: The solution of dynamic contact (elastic impact) problems is complicated by the changing nature of the contact area. If a finite element approach is used, the system matrices vary with the contact area. If the problem is properly formulated, such changes are rank one. Rank one changes produce easily determinable changes in the LDLT decompositions of the system matrices. This renders a class of dynamic contact problems soluble with the same accuracy and computational effort as is associated with the solution of any dynamic problem using finite element procedures. Consideration of an example problem involving the impact of spring-mass systems confirms this claim.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 101-119 
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    Keywords: Engineering ; Engineering General
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    Notes: A method is described to derive finite element schemes for the scalar convection equation in one or more space dimensions. To produce accurate temporal differencing, the method employs forward-time Taylor series expansions including time derivatives of second- and third-order which are evaluated from the governing partial differential equation. This yields a generalized time-discretized equation which is successively discretized in space by means of the standard Bubnov-Galerkin finite element method. The technique is illustrated first in one space dimension. With linear elements and Euler, leap-frog and Crank-Nicolson time stepping, several interesting relations with standard Galerkin and recently developed Petrov-Galerkin methods emerge and the new Taylor-Galerkin schemes are found to exhibit particularly high phase-accuracy with minimal numerical damping. The method is successively extended to deal with variable coefficient problems and multi-dimensional situations.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 175-181 
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    Keywords: Engineering ; Engineering General
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 169-173 
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    Notes: A two-noded, straight element with one-point quadrature is introduced for the unsymmetrical bending of orthotropic axisymmetric shells. The numerical results indicate that the present simple element performs accurately for both thick and thin shell applications.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 187-194 
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    Notes: Numerical results using uniform h-refinement approaches to the solution of a pair of elasticity problems with singularities are presented and compared to recently published results based on the uniform p-refinement approaches.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 143-167 
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    Notes: Steady-state, free surface seepage through a heterogeneous porous medium underlain by a drain at a finite depth is solved using a fixed domain solution technique. The problems investigated are axisymmetric seepage from a circular pond and plane seepage from a symmetric channel. These ponds and channels may have variable shaped bottoms. Since the Baiocchi transformation was used to define a new dependent variable, the form of the permeability function was restricted to a product of functions of the independent variables. Herein the permeability was chosen to be a function of the depth only. For certain forms of this function, namely those having negative gradients, part of the flowfield becomes unsaturated and this violates the assumed saturation of the flowfield in the flow theory. The governing differential equation, which holds in the sense of distributions, is derived for a fixed solution domain and a simple algorithm (a finite difference successive over-relaxation scheme with projection) is given to obtain the solution to these free surface problems. Numerous comparisons are made with published results. Rigorous mathematical justification of the methods used herein can be found in the references cited.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 195-195 
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    International Journal for Numerical Methods in Engineering 20 (1984) 
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 182-186 
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    Notes: Bathe's basic algorithm of subspace iteration for the solution of the symmetric eigenvalue problem is improved by including a Chebyshev filtering mechanism. To obtain satisfactory convergence for the largest eigenvalues, a shifting strategy is adopted. The shift factor is approximately computed by the Lanczos process.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 479-503 
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    Keywords: Engineering ; Engineering General
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    Notes: A transfer-matrix analysis is presented for determining the static behaviour of thick beams of ‘multimodular materials’ (i.e. materials which have different elastic behaviour in tension and compression, with nonlinear stress-strain curves approximated as piecewise linear, with four or more segments). To validate the transfer-matrix method results, a closed-form solution is also presented for cases in which the neutral-surface location is constant along the beam axis. Numerical results for axial displacement, transverse deflection, bending slope, bending moment, transverse shear, axial force and location of neutral surface are presented for multimodular and bimodular models of unidirectional aramid cordrubber. The transfer-matrix method results agree very well with the closed-form solutions.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 523-528 
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    Notes: An 8-node brick element based on the assumed stress hybrid formulation is described. With three additional stress fields, the element stiffness matrix now has the required rank of 18, and the ‘bending’ response is exact with rectangular elements. Surprisingly, the 2 × 2 × 2 Gauss rule suffices for all numerical integrations, to satisfy the constant stress patch test.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 529-548 
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    Notes: An assumed stress hybrid curvilinear triangular finite element is described which is based upon the Kirchhoff theory of plate bending. The derivation extends the assumed stress hybrid technique to curvilinear boundaries where the twelve connectors are related to those of an equilibrium rectilinear element and to Semiloof. The solution process demands only first derivatives of the shape functions.The element is subjected to various patch tests for constant bending, e.g. where the central element is in close approximation to a circle. All tests are passed for stress couples and vertex displacements, but values of the remaining connectors do not resemble exact results. Patch tests for rigid-body movements are passed exactly in every respect.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 505-514 
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    Notes: A numerical technique has been developed to solve a system that consists of m linear parabolic differential equations with coupled nonlinear boundary conditions. Such a system may represent chemical reactions, chemical lasers and diffusion problems. An implicit finite difference scheme is adopted to discretize the problem, and the resulting system of equations is solved by a novel technique that is a modification of the cyclic odd-even reduction and factorization (CORF) algorithm. At each time level, the system of equations is first reduced to m nonlinear algebraic equations that involve only the m unknown grid points on the nonlinear boundary. Newton's method is used to determine these m unknowns, and the corresponding Jacobian matrix can be computed and updated easily. After convergence is achieved, the remaining unknowns are solved directly. The efficiency of this technique is illustrated by the numerical computations of two examples previously solved by the cubic spline Galerkin method.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 549-553 
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    Notes: It is demonstrated that finite element discretizations may not, in general, satisfy the maximum principle; and sufficient conditions are presented to guarantee that solutions based on bilinear elements do satisfy the maximum principle.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 515-522 
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    Notes: The efficiency and computational accuracy of the boundary element and finite element methods are compared in this paper. This comparison is carried out by employing different degrees of mesh refinement to solve a specific illustrative problem by the two methods.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 565-572 
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    Notes: The BEM is shown to be superior to FEM with respect to economy (man hour and data processing) and convergence (with increasing number of boundary nodes) in the elasic notch stress analysis of compact components.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 555-563 
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    Notes: This note discusses the numerical solution of the kinematic wave equation under those conditions when the solution contains a discontinuous shock. A finite element solution is described in which shocks are represented by discrete nodal discontinuities. The implementation of the method follows conventional finite element practice over the shockless regions of the solution domain which are coupled by frontal constraints. The basis of the method and examples of its application to the solution of the kinematic wave equation in one and two dimensions are presented.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 887-914 
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    Notes: A concise survey of formulation methods of geometric and material nonlinearity problems is given. Differences between existing formulations in the literature are discussed. A consistent Lagrangian and updated Lagrangian formulations are derived from the energy balance equation transformed to the proper reference configuration. Differences between the existing formulations and similar ones in the literature are found to be in specific geometric nonlinear terms in the final incremental equation as well as in the definition of the load increment vector. Specific forms of constitutive equations for elastic and elastoplastic materials are presented. Some of the forms used by other authors in application to elastoplastic materials are shown to be inconsistent. Sample numerical examples intended for checking some particular points in the formulation are presented.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 845-849 
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    Notes: Two numerical methods for solving two-point boundary-value problems associated with systems of first-order nonlinear ordinary differential equations are described. The first method, which is based on Lobatto quadrature, requires four internal function evaluations for each subinterval. It does not need derivatives and is of order h7, where h is the space chop. The second method, which is similar to the first but is based on Lobatto-Hermite quadrature, makes the additional use of derivatives to achieve O(h9) accuracy. Results of computational experiments comparing these methods with other known methods are given.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 915-929 
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    Notes: An almost linear optimization problem of importance in vibration isolation has been identified and algorithms were developed to minimize the forced vibrational response of structural systems. The constraints can be either displacements or accelerations. These algorithms have been studied for transient response, frequency response and stationary random using the direct dynamic solution. Multiple response points and loading conditions may be used.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 949-966 
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    Notes: When applied to large sparse sets of simultaneous equations, classical iterative methods may yield very poor convergence rates. This paper gives an incomplete Choleski-conjugate gradient algorithm (ICCG) which has reliably good convergence rates at the expense of computing and using at each iteration an incomplete Choleski factor of the coefficient matrix. The method is applicable to any problems in which the coefficient matrix is symmetric positive definite and is likely to be advantageous with respect to elimination when it is not possible to represent the equations in a dense band form.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 931-948 
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    Notes: A method is presented for performing efficient and stable finite element calculations of heat conduction with quadrilaterals using one-point quadrature. The stability in space is obtained by using a stabilization matrix which is orthogonal to all linear fields and its magnitude is determined by a stabilization parameter. It is shown that the accuracy is almost independent of the value of the stabilization parameter over a wide range of values; in fact, the values 3, 2 and 1 for the normalized stabilization parameter lead to the 5-point finite difference, 9-point finite difference and fully integrated finite element operators, respectively, for rectangular meshes; numerical experiments reported here show that the three have identical rates of convergence in the L2 norm. Eigenvalues of the element matrices, which are needed for stability limits, are also given. Numerical applications are used to show that the method yields accurate solutions with large increases in efficiency, particularly in nonlinear problems.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 979-979 
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 980-980 
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 967-977 
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    Notes: A simple dynamic finite element algorithm for analysing a propagating mixed mode crack tip is presented. A double noding technique, which can be easily incorporated into existing dynamic finite element codes, is used together with a corrected Ĵ integral to extract modes I and II dynamic stress intensity factors of a propagating crack. The utility of the procedure is demonstrated by analysing test problems involving a mode I central crack propagating in a plate subjected to uniaxial tension, a stationary slanted central crack in a plate subjected to uniaxial inpact loading and an extending slanted-edge crack in plate subjected to uniaxial tension.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 1370-1371 
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 1407-1414 
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    Notes: The paper describes the development of an eight-node plane rectangular finite dynamic element and presents detailed descriptions of the associated numerical formulation involving the higher order dynamic correction terms pertaining to the related stiffness and inertia matrices. Numerical test results of free vibration analyses are presented in detail for the newly developed eight-node element and also the corresponding four-node element in order to make a clear comparison of the relative efficiencies of the corresponding finite element and dynamic element procedures. Such results indicate a superior pattern of solution convergence of the presently developed dynamic element.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 1415-1431 
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    Notes: A computational strategy for the evaluation of delamination stresses in multilayered fibre-reinforced composites is presented. The approach uses special inter-laminar shear elements and an iterative procedure in the calculation of out-of-plane stresses. Both regular and singular elements for the free edge are presented. The solutions are carried on by switching between direct matrix solution methods of small systems of equations and an iterative conjugate gradient method. The proposed discretization avoids ill-conditioning problems, modelling difficulties and large computational cost associated with three-dimensional analysis of delamination of holes, cut-outs, joints and areas of complex stress states.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 1433-1441 
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    Notes: A general, consistent and accurate procedure for obtaining difference equations from differential equations is presented and illustrated by several examples. The procedure is an extension of the integral method and incorporates what is known of the solution to the differential equation in the formation of the corresponding difference equation. For the same accuracy, large increases in grid size over that obtained by standard Taylor's series approximations are possible. Both boundary-value and initial-value problems are treated.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 1443-1459 
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    Notes: The standard isoparametric transformation for finite element analysis is shown to be only a special case of a more general transformation which allows for flexibility in locating side and interior element nodes. Side nodes positioned at the same relative distance from corner nodes in both local and global space, and interior nodes positioned such that they do not affect the Jacobian of the co-ordinate transformation, provide improved accuracy over nodes positioned locally without regard for the global configuration. This concept is demonstrated in the context of two-dimensional quadratic quadrilateral isoparametrics, but is also applicable to higher orders and dimensions. In the test cases examined, the new formulation provides a reduction in error of up to three orders of magnitude and also provides accurate solutions for nodal placements which are not allowed using the standard transformations.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 1461-1475 
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    Notes: A weighting function by which the mass matrix is made to be diagonal is deduced on the two-dimensional simplex element. Adopting the weighted residual method with the deduced weighting function for spatial domain and the two-step Lax-Wendroff method for time differentiation, the two-dimensinoal tidal flow in a model basin is calculated by means of the explicit finite element method. The water mass transport integrated over one tidal period at some cross-sections of the basin is also estimated, in order to verify the water mass conservation. It is found that the water mass is nearly conserved in the present method, although the usual lumped mass matrix technique fails to conserve.The present explicit finite element method with the conservative form of governing equations is also investigated and the water mass conservation is found to be a little more improved in this case than in the case of non-conservative forms. Application to a real bay indicates that the method with the conservative form of equations could be used for the estimation of tidal residual flow.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 1477-1489 
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    Notes: In this work, the plastic stress-strain relation and the incremental elastoplastic stiffness matrix for materials obeying the principal stress yield criterion are derived in the context of the associated theory of plasticity. Particular attention is paid to the treatment of the singularity conditions associated with this yield criterion.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 1491-1506 
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    Notes: It is demonstrated that the residual in a compatible (displacement) finite element solution can be partitioned into local self-equilibrating systems on each element. An a posteriori error analysis is then based on a complementary approach and examples indicate that the guaranteed upper bound on the energy of the error is preserved.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 1507-1519 
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    Notes: The paper develops a new discrete transform pair which economizes on the number of samples of F(s) and f(t) compared with the standard pair. Whereas the standard pair used uniform sampling along the Bromwich contour, the new method allows a progressive increase in the sampling interval, thereby reducing the number of samples required to meet a specified bandwidth requirement. In the case of the computation of transient responses of physical systems, a reduction by a factor of 10 is not unrealistic. The new transform pair is based on the generalization of fundamental sampling principles. In particular, this leads to an integration contour different from the Bromwich contour.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 1553-1554 
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    Keywords: Engineering ; Engineering General
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 1581-1597 
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    Notes: A new family of coupled time integration operators of different orders is developed. It permits different time integration operators to be used simultaneously in various regions of the model. This approach has the advantage of applying a very accurate time integrator, such as Runge Kutta method of order two to four, to the local nonlinear region of interest and a simple time integrator, such as the implicit-explicit or mixed time implicit-explicit method of order one, for the remaining region. The computer implementation aspects, stability analysis as well as the numerical evaluations of these new methods are presented. This is another attractive addition to the repertoire of time integration methods.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 1613-1628 
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    Notes: A finite element formulation for elastoviscoplasticity problems in the presence of large strains and deformations is presented in this paper. The formulation is capable of using any of a class of combined creep-plasticity constitutive models with state variables for the description of material behaviour. The specific problem considered is plane strain extrusion using the constitutive model originally proposed by Hart. Numerical results are presented for sample problems and the important effects of extrusion speed and friction (at the die-workpiece interface) on the residual stresses in an extruded sample are investigated. The computer program developed here is quite general and can be modified to solve problems for other manufacturing processes like rolling, sheet forming, etc.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 1643-1660 
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    Keywords: Engineering ; Engineering General
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    Notes: A new direct search package is presented. Typical test functions solved establish the robustness and efficiency of the package. The most significant features of the package are that it works satisfactorily even in single precision and requires much less memory. It needs only the function values and the unidimensional search is totally dispensed with. Methods such as different descent techniques, the RMS procedure, the co-ordinate movement technique and the gallop-canter equalization scheme form the sequence of strategies.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 1629-1641 
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    Keywords: Engineering ; Engineering General
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    Topics: Mathematics , Technology
    Notes: This paper attempts to answer two commonly raised questions during the preparation of a finite element mesh, for the linear elastic fracture analysis of cracked structure: how to set up the finite element mesh around the crack tip, and what level of accuracy is to be expected from such a modelling.Two test problems, with known analytical expressions for their stress intensity factors, are analysed by the finite element method using the isoparametric quadratic singular element. The modified parameters were the order of integration, aspect ratio, number of elements surrounding the crack tip, use of transition elements, the singular element length over the total crack length, the symmetry of the mesh around the crack tip. Based on these analyses, a data base is created and various plots produced. The results are interpreted, the accuracy evaluated and recommendations drawn.Contrary to previous reports, it is found that the computed stress intensity factor (SIF) remains within engineering accuracy (10 per cent) throughout a large range of l/a (singular element length over crack length) for problems with a uniform non-singular stress distribution ahead of the crack tip (i.e. double edge notch), and l/a should be less than 0·1 for problems with a non-singular stress gradient (i.e three-point bend). Also, it is found that the best results are achieved by using at least four singular elements around the crack tip, with their internal angles around 45 degrees, and a reduced (2 × 2) numerical integration.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 1661-1670 
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    Keywords: Engineering ; Engineering General
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    Notes: This paper presents and analyses methods for design sensitivity analysis of static response for structural systems when general boundary conditions are used during the analysis phase. The design sensitivity analysis methods where the boundary conditions are used to reduce the equilibrium equation have been presented earlier. In such procedures, the reduced stiffness matrix is non-singular. This fact is used to show differentiability of the displacement vector. When reduction procedures are not used, the global structural stiffness matrix may be singular. In such a case, differentiability of the displacement vector may be questionable. However, it is shown that the displacement vector is still differentiable. Several forms of the structural design sensitivity equations are presented. Although these equations are derived with Lagrange multiplier treatment of general boundary conditions, it is shown that they are applicable with any treatment of general boundary conditions. For example, they can also be used with co-ordinate reduction schemes. Therefore, design sensitivity analysis can be carried out with any existing finite element analysis computer code. Moreover, the procedures presented can be used to calculate sensitivity of a force of constraint can that is desired. The formulations are kept quite general such that design dependent boundary conditions can be routinely treated. An example is used to illustrate the procedures.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 1671-1683 
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    Notes: In this paper, an efficient, exact zooming technique is developed which employs static condensation and exact structural reanalysis methods. For a multiple level of zooming such that the successive level of zooming is contained within the prior levels of zoom, repeated application of static condensation will reduce the system to one that is associated only with the degrees-of-freedom (dof) of the original model. Then, application of an exact static reanalysis technique permits the displacements at the dof of the original model that are contained in the final zoomed portion of the structure to be obtained first. Next, the response external to the zoom, as well as the response of additional dof within various levels of zooming, can be computed. With the triangular factor of the stiffness matrix of the original system available, this approach involves only the solution of a system of equations of small order. The proposed method is demonstrated by a numerical example.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 1697-1702 
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    Notes: An inversion formula based on expansion in a Fourier sine series, proposed by Koizumi in 1935, is examined. It is found to give results of moderate accuracy with relatively little work.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 1685-1695 
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    Notes: A new method for the formulation of hybrid elements by the Hellinger-Reissner principle is established by expanding the essential terms of the assumed stresses as complete polynomials in the natural coordinates of the element. The equilibrium conditions are imposed in a variational sense through the internal displacements which are also expanded in the natural co-ordinates. The resulting element possesses all the ideal qualities, i.e. it is invariant, it is less sensitive to geometric distortion, it contains a minimum number of stress parameters and it provides accurate stress calculations. For the formulation of a 4-node plane stress element, a small perturbation method is used to determine the equilibrium constraint equations. The element has been proved to be always rank sufficient.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 1703-1709 
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    Notes: The strain space and traditional stress space formulations of plasticity theory are compared. We show how plasticity theory has been implemented numerically to solve static and dynamic problems, and conclude that the numerical loading criteria that have been used for two decades are identical to the strain space loading criteria proposed by Naghdi and co-workers.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 1729-1733 
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    Notes: A PERQ single user computer can be used to animate structure plots, which is of great assistance when interpreting the results from finite element programs. Excellent appreciation may be obtained of a structure oscillating in complicated natural modes. The technique is also useful for studying deformations due to static forces.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 1711-1728 
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    Notes: Simple solutions are extracted from the exact compatibility equations of inextensional bending for a special class of shell shapes, and it is speculated that these solutions provide useful criteria towards evaluating finite elements for these and closely related shapes. Curved finite elements of a quadratic parametric representation, which do not belong to the special class, are then studied. By using a series of exact inextensional bending displacements, it is concluded from numerical evidence that the criteria apply to finite element shapes in this representation when they are shallow, as is usually the case in practice. Criteria for homogeneous membrane actions follow from Gol'denveizer's static-geometric analogue.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 1745-1749 
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    Notes: Using the partial-boundary element approach, in which only a part of the boundary in problem is discretized, the elastic torsion problem of a prismatic beam of a cracked rectangular cross-section has been solved in this brief paper, together with a numerical example to show the accuracy and versatility of the approach.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 1751-1755 
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    Notes: The radial symmetry of the cable results in circular finite elements to evaluate the potential distribution in a multi-dielectric cable. Owing to this, a two dimensional analysis used in conjunction with triangular finite elements is reduced to one-dimensional analysis. This method therefore suggests an alternative proposal to calculate the potential distribution in a multi-dielectric cable with better accuracy.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 1756-1756 
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 1757-1757 
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 1757-1758 
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 1758-1759 
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    International Journal for Numerical Methods in Engineering 20 (1984) 
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 1791-1796 
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    Notes: Contour plots of finite element solutions, especially for derivatives, often are discarded due to poor plotting algorithms. This note shows how a smoothing scheme based on a minimum surface theory can significantly improve the quality of the graphical results. The scheme proposed is compared with many other smoothing schemes, including least squares, on a simple problem.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 1763-1790 
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    Notes: This paper describes the stages of development of an optimum structural design scheme. Any attempt to generate the optimum scantlings of a structure of even moderate complexity usually poses nonlinear programming problems for which there exist no universally accepted solution method. The structure considered here is a complete compartment of a single-hulled ship acted upon by multiple load cases. The elastic responses under four different load types are obtained by a quasi finite element analysis routine developed specifically for this purpose. Sequential linear programming (SLP) with appropriate artifices to achieve rapid convergence has been successfully employed as an optimization routine to minimize the object function, which in this case was the total weight of the major structural members. The test problem has 490 basic degrees-of-freedom, 21 free design variables and more than 100 mixed constraints, the majority of which are nonlinear implicit stress functions. The method is then applied to a number of cases representative of small warship designs. Suggestions for future extensions and general conclusions are included at the end of the paper.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 1815-1821 
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    Notes: The approach of Cermak and Zlamal1 for solving quasilinear parabolic equations is modified and improved. The modified approach leads to a normal system of ODE, which may be solved with a standard program. The numerical solution of a specially selected example is compared with the exact solution. The method is applied on a system of quasilinear parabolic equations, which describes a real process - crystallization of a metal ingot.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 1797-1813 
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    Notes: The simulation of a large-scale gas transmission network involves the numerical solution of a large system of initial valued, stiff algebraic/differential equations. Rapid changes in the solution are present due to the disturbances generated by the varying consumer demand and the operation of network controlling devices such as compressors. This paper discusses the design of an efficient variable-step integrator for the solution of the problem. Two sets of strategies are presented for implementing the variable-step integrator; one for the implicit numerical method such as the diagonally implicit Runge-Kutta methods, and the other for the linearly implicit Rosenbrock-type method. The performance of the numerical methods implemented are compared with the British Gas simulation program PAN on a number of large, realistic transmission networks.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 1823-1840 
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    Notes: The optimality conditions for the optimal shape remodelling of linearly elastic plates are obtained by introducing the total variation of a function defined on a variable domain, although the variation of a function has been taken on a fixed domain in most literature on calculus of variations. Using these optimality conditions, a solution scheme involving an iterative algorithm is proposed, together with several numerical examples.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 1863-1884 
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    Notes: This paper presents a simple scheme for analysing shells subject to the Kirchhoff-Love hypothesis. The method combines a previously developed, statically determinate truss model with the static-geometric analogy of elastic thin shell theory. In this way, both in-plane and flexural mid-surface actions are obtained by simply repeating the elementary computations performed on an essentially ‘membrane’ truss.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 1841-1861 
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    Notes: This paper presents the modelling of open shells, such as those used in roof construction, by means of a single-layered, pin-jointed truss. The latter exhibits several advantages over existing framework models, such as, for example, statical determinacy and the capacity to cope with arbitrary material constants. In the present article, the truss model is developed for and applied to the numerical analysis of shells subject to the membrane hypothesis. However, the same statically determinate model forms the basis for a computational scheme for analysing shells according to the more formal, statically indeterminate bending theory; the latter application of the truss model will be presented in a subsequent paper.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 1951-1952 
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 1952-1952 
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 1957-1958 
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 1953-1956 
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    International Journal for Numerical Methods in Engineering 20 (1984) 
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 1959-1964 
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    Notes: A new method for static condensation is presented. This method utilizes Choleski decomposition with some modification to obtain the effective stiffness matrix and the effective load vector. A comparison between this modified decomposition method and the usual matrix decomposition method shows that the former is more efficient than the latter.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 1965-1990 
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    Notes: An approach for the fully automatic generation of three-dimensional finite element meshes is presented. The method is specifically designed for use with solid modelling systems which provide a complete and unique definition of the geometry of a part. The method follows from the basic concepts of the octree encoding technique with specific modifications made to produce valid, user controllable finite element meshes. Example problems are included to demonstrate the technique.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 2065-2075 
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    Notes: A method which combines the finite element technique and the singular-integral equation method is presented. The association of the two methods is obtained with the help of Schwarz's alternating method (SAM). The method was applied with satisfactory results to the solution of a series of problems of a circular arc crack lying inside a finite thin plate for various lengths of the circular arc and for various dimensions of the rectangular cracked sheet.
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    International Journal for Numerical Methods in Engineering 20 (1984) 
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 2121-2141 
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    Notes: A 48 degrees-of-freedom (d.o.f.) quadrilateral thin elastic shell finite element using variable-order polynomial functions, B-spline functions and rational B-spline functions to model the shell surface is developed. This development may allow the stiffness formulation of the shell element to be linked to the geometry data bases created by computer aided design systems. The displacement functions are that of bicubic Hermitian polynomials. The displacement functions and d.o.f. are expressed and investigated in both the curvilinear and Cartesian forms. The cuivilinear form is simpler and can provide the proper solution for a certain class of shell problems. For certain highly curved shells such as bellows, however, the curvilinear form fails to properly model some rigid body modes even with either the explicit inclusion of rigid body terms or the high order displacement functions. It is suggested in this study that such difficulty can be circumvented and the rigid body modes can be properly included if a Cartesian form is used for displacement functions. The strain-displacement equations are expressed in curvilinear co-ordinates. Thus, the Cartesian displacement functions require a transformation to curvilinear displacement at each numerical integration point. Examples include a pinched cylinder, a translational shell under central load, a uniformly loaded hypar shell, a pressurized ovel shell, a semi-toroidal bellows and a U-shaped bellows. For the first four examples, geometric modellings consist of polynomials of second-order (subparametric), third-order (isoparametric), and fourth and fifth-order (both superparametric) as well as B-spline functions of fourth- and fifth-order. The geometries of the pinched cylinder, the semi-toroidal bellows, and the U-shaped bellows were modelled exactly using rational B-spline functions. All the results obtained are in good agreement with alternative existing solutions.
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    International Journal for Numerical Methods in Engineering 20 (1984), S. 2175-2195 
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    Notes: A finite element method for contact problems in crack mechanics is developed on the basis of the penalty function method. The method is successfully applied to three important problems in fracture mechanics: a crack propagated from a pin hole, a two-point supported specimen with an edge crack loaded by a stamp, and a thick plate with a through-wall crack under bending force.
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  • 95
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    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 20 (1984), S. 2253-2281 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: A total Lagrangian finite element formulation for the geometrically nonlinear analysis (large displacement/large rotations) of shells is presented. Explicit expressions of all relevant finite element matrices are obtained by means of the definition of a local co-ordinate system, based on the shell principal curvature directions, for the evaluation of strains and stresses. A series of examples of nonlinear analysis of shell and plate structures is given.
    Additional Material: 20 Ill.
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  • 96
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    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 20 (1984), S. 2283-2301 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: A combined iterative method is formulated for the partial solution of the eigenproblem Ax = λBx that arises from the application of the finite element method. The method is a combination of the conjugate gradient (CG) method and the symmetric successive co-ordinate overrelaxation (SSCOR) method. Both of these methods belong to the same family of iterative methods which retain their vectorial form and approach the solution by seeking stationary points of the Rayleigh quotient. The formulation consists in splitting a characteristic matrix into the sum of two matrices, one of which corresponds to the SSCOR matrix, and then accelerating the associated iteration using the CG. The behaviour of the method is illustrated for five test cases and comparisons are made with other iterative methods. The influence of the relaxation parameter ω on the computational efficiency of the methods is also studied.
    Additional Material: 5 Ill.
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  • 97
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    Electronic Resource
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 20 (1984), S. 2303-2309 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: Part 1 of this paper1 presents a very general single step algorithm SSpj for the numerical integration of first and second order time dependent differential equations. In Part 2 we present and discuss results of the accuracy and stability analysis for SS11, SS21, SS31, SS22 and SS32. There is also a detailed comparison of SS22 and the Newmark algorithm.
    Additional Material: 2 Tab.
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  • 98
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    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 20 (1984), S. 2325-2325 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Type of Medium: Electronic Resource
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  • 99
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    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 20 (1984), S. 241-253 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: Recently developed computer aided design systems for the design and modification of complex physical solids using interactive computer graphics offer the exciting possibility of an integrated design/analysis system. Called geometric modellers, these systems build complex solids from primitive solids (cubes, cylinders, spheres, etc.) and macro solids (combinations of primitives). To provide an effective finite element analysis capability for these systems, methods must be devised to ease the burden of discretizing the solid geometry into a user controlled finite element mesh. In this paper we describe a new class of transitional blended finite elements which make substantially simpler the task of finite element mesh generation and local mesh refinement. Computational experience indicates that numerical accuracy is not compromised by use of these flexible elements.
    Additional Material: 19 Ill.
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  • 100
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    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 20 (1984), S. 297-304 
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: A non-iterative method is presented for solving a system of algebraic equations forming a banded matrix with each diagonal submatrix being tridiagonal and all other submatrices being diagonal. A recursion relation is assumed between successive elements of the solution vector. This relation is determined by equating a linearly combined form of it with the original set of equations also being linearly combined. The solution vector is determined by back-substitution of elements in the recursion relation. Since the method takes advantage of the banded structure of the matrix, it is more efficient than the conventional methods. A FORTRAN program that incorporates the present method is included.
    Additional Material: 1 Ill.
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