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  • temperature  (296)
  • fish  (255)
  • Springer  (545)
  • American Institute of Physics (AIP)
  • 2010-2014
  • 2000-2004  (103)
  • 1995-1999  (442)
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  • 1
    ISSN: 1570-7458
    Keywords: Anagyrus kamali ; Encyrtidae ; parasitoid ; Maconellicoccus hirsutus ; Pseudococcidae ; host density ; functional response ; temperature ; photoperiod
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The peformance of the parasitoid Anagyrus kamali Moursi [Hymenoptera: Encyrtidae], as a function of host density, temperature, and photoperiod was investigated with the objective to optimize a mass-rearing system in the context of a biological control program. The number of hosts parasitized at densities varying from 2–100 hibiscus mealybug (HMB), Maconellicoccus hirsutus Green [Homoptera: Pseudococcidae], corresponded to a type II-III functional response in fixed-time conditions and a type III in variable-time conditions. Twenty-six percent of the oviposited eggs led to progeny emergence with a sex ratio of 0.49±0.102 (M/F), regardless of host density. Fecundity and oviposition period under six abiotic combinations (i.e., two temperatures (26±2 °C and 32±2 °C) and three photoperiods (L0:D24, L12:D12, L24:D0)) were measured. Lifetime fecundity and reproductive life were significantly affected by temperature and photoperiod conditions. Optimum female parasitoid lifetime fecundity was attained at 26±2 °C, L0:D24 with an average of 116.1±17.43 eggs. At 32±2 °C, L24:D0 and L12:D12, an average of 79.4±34.57 and 85.8±35.81 eggs were laid, respectively. Reproductive longevity was maximal at 26±2 °C, L0:D24 with 12±4.85 days of oviposition. Because the parasite A. kamali can be reared optimally without light, this may save tremendous energy costs.
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  • 2
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    Entomologia experimentalis et applicata 95 (2000), S. 173-184 
    ISSN: 1570-7458
    Keywords: Aphidius ervi ; Aphidius rhopalosiphi ; Praon volucre ; Sitobion avenae ; temperature ; development time ; parasitization ; superparasitization ; lower temperature thresholds ; immature mortality ; sex ratio
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Temperature dependencies were established for the egg-to-mummy and mummy-to-adult phases, for mummy mortality, and for parasitism of Aphidius ervi Haliday, Aphidius rhopalosiphi De Stefani-Perez, and Praon volucre (Haliday) (Hymenoptera, Aphidiidae), three parasitoids of Sitobion avenae (Fabricius) (Homoptera, Aphididae), at 8 °C, 12 °C, 16 °C, 20 °C, and 25 °C on winter wheat (cv. Haven). A physiological model described temperature-dependent development over the full temperature range, whereas a linear model was fitted for data above 8 °C and used to estimate the lower temperature thresholds and day-degrees (° D) required for development. The thresholds for A. ervi were 2.2 °C for egg-mummy development and 6.6 °C for mummy-adult development, those for A. rhopalosiphi were 4.5 °C and 7.2 °C, and those for P. volucre were 3.8 °C and 5.5 °C. The time to develop into mummies and adults differed significantly between the three species: A. ervi development into mummies required an average of 159 ° D, while development into adults took an average of 73 ° D. The corresponding average times required for A. rhopalosiphi and P. volucre to develop mummies were 124° D and 126° D, while their development into adults required an average of 70° D and 150° D, respectively. Mummy mortality was 25–35% at 8 °C and less at the higher temperatures tested, but began to increase again at 25 °C, showing a quadratic relationship between mortality and temperature. Parasitization was very low or, in the case of P. volucre, absent up to 12 °C and thereafter increased with increasing temperature. The relationship between parasitization, recorded as percent aphids mummified, and temperature was linear at the temperatures tested and depended on species. A. ervisuperparasitized 11.1% aphids at 20 °C and 16.6% aphids at 25 °C, whereas superparasitism was low in A. rhopalosiphi and absent in P. volucre. From 16 °C to 25 °C the P. volucre sex ratio increased. For A. ervi and A. rhopalosiphi there was no trend with temperature, but at 20 °C and 25 °C it was close to even. Field data for 1996 and 1997 allowed for a comparison of actual and expected emergence of overwintering mummies. In both years, parasitoids were predicted to have emerged from overwintering mummies well in advance of the onset of aphid infestation, and more than a month earlier than the first parasitized aphids were found in winter wheat. Observations from trap plants in other crops supported the predictions of the models. Other factors that can affect biological control by cereal aphid parasitoids are discussed.
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  • 3
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    Journal of solution chemistry 29 (2000), S. 63-86 
    ISSN: 1572-8927
    Keywords: Thorium ; hydrolysis ; potentiometry ; solvent extraction ; temperature ; thermodynamics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract The stability constants of thorium(IV) hydrolysis species have been measured at15, 25, and 35°C (in 1.0 mol dm−3 NaClO4) using both potentiometry and solventextraction. The results indicate the presence of the monomeric speciesTh(OH)3+, Th(OH)2+ 2, Th(OH)+ 3, and Th(OH)4, in addition to the polymericspecies Th4(OH)8+ 8 and Th6(OH)9+ 15. The polymeric species were found to beimportant, although the total thorium concentration was limited to 0.01–0.1mmol-dm−3. The solvent extraction measurements required the use of acetylacetone.As such, the stability constants of thorium(IV) with acetylacetone were alsomeasured using both potentiometry and solvent extraction. All logarithms of thestability constants were found to be linear functions of the reciprocal absolutetemperature indicating that ΔH o and ΔSo of reaction are both independent oftemperature (over the temperature range examined in the study).
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  • 4
    ISSN: 1572-8927
    Keywords: Flow-through cell ; conductivity ; electrolyte ; ion association ; temperature ; pressure ; LiCl ; NaCl ; KCl
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A flow-through electrical conductance cell was assembled in order to measuremolar conductances of dilute aqueous electrolytes with a high degree of accuracyat high temperatures and pressures. The design of the cell is based on the conceptdeveloped at the University of Delaware and built in 1995, with modificationsthat will allow the cell to operate at much higher temperatures (to 600°C) andpressures (to 300 MPa). At present, the cell has been tested successfully bymeasuring aqueous (10−4-10−3 mol-kg−1) solutions of LiCl, NaCl, and KCl attemperatures 25–410°C and pressures 9.8–33 MPa. The results are in goodagreement with reported values, including those measured with the Delawareflow-through cell. These new results are also complementary to our previousresults, which were measured with a static high-pressure cell. Measurements attemperatures near the critical point of water (374°C, 22.1 MPa) require the useof lower solution concentrations that were unachievable in the past with thestatic cell.
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  • 5
    ISSN: 1573-0417
    Keywords: diatoms ; climate change ; temperature ; pH ; transfer functions ; lake sediments
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences
    Notes: Abstract The relationships between diatoms (Bacillariophyceae) in surface sediments of lakes and summer air temperature, pH and total organic carbon concentration (TOC) were explored along a steep climatic gradient in northern Sweden to provide a tool to infer past climate conditions from sediment cores. The study sites are in an area with low human impact and range from boreal forest to alpine tundra. Canonical correspondence analysis (CCA) constrained to mean July air temperature and pH clearly showed that diatom community composition was different between lakes situated in conifer-, mountain birch- and alpine-vegetation zones. As a consequence, diatoms and multivariate ordination methods can be used to infer past changes in treeline position and dominant forest type. Quantitative inference models were developed to estimate mean July air temperature, pH and TOC from sedimentary diatom assemblages using weighted averaging (WA) and weighted averaging partial least squares (WA-PLS) regression. Relationships between diatoms and mean July air temperature were independent of lake-water pH, TOC, alkalinity and maximum depth. The results demonstrated that diatoms in lake sediments can provide useful and independent quantitative information for estimating past changes in mean July air temperature (R2 jack = 0.62, RMSEP = 0.86 °C; R2 and root mean squared error of prediction (RMSEP) based on jack-knifing), pH (R2 jack = 0.61, RMSEP = 0.30) and TOC (R2 jack = 0.49, RMSEP = 1.33 mg l-1). The paper focuses mainly on the relationship between diatom community composition and mean July air temperature, but the relationships to pH and TOC are also discussed.
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  • 6
    ISSN: 1349-9432
    Keywords: optical fibres ; temperature ; sensors ; tantalum pentoxide ; thermal optic ; thermal expansion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract A fiber optic low-coherence sensor based on the spectral shift of tantalum pentoxide thin films for absolute temperature sensing up to 650°C is described. A tantalum pentoxide single layer was deposited directly onto the cleaved end-face of a single mode optical fibre and was illuminated with an super luminescence diode (SLD) source through a directional coupler. Interference fringes of the film on reflection were obtained within the optical bandwidth of the SLD using an optical spectrum analyser. The spectral shift versus temperature rise showed no turning points and the output was unambiguous, linear, monotonic and gave about 0.016 nm wavelength shift in the spectrum per°C. A semi-empirical calibration procedure based on the refractive index (n) and thickness (l) of the tantalum pentoxide film for absolute thermometric measurements is described.
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  • 7
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    Optical review 7 (2000), S. 555-560 
    ISSN: 1349-9432
    Keywords: lidar ; remote sensing ; Rayleigh scattering ; wind ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Physics
    Notes: Abstract A new method of simultaneous remote sensing of atmospheric wind and temperature by a ultraviolet Rayleigh lidar is described. This technique uses two narrowband filters located at either side of the wings of the Rayleigh backscatter spectrum to analyze Rayleigh backscattering signals. These filters are selected to be greatly sensitive to both velocity and temperature. By measuring the ratio and the sum of the two normalized filtered signals, the line-of-sight wind velocity and temperature profiles can be retrieved. A lidar system is proposed for the wind velocity and temperature measurements in the middle atmosphere, and the simulation results show that the accuracies of velocity and temperature are about 1 m/s and 2 K at the height of 30 km, respectively. The influence of aerosol component has been estimated for clear weather conditions, and with an uncertainty of aerosol component of 15% the errors are about 0.1 m/s and 2 K above the troposphere, respectively.
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  • 8
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    Journal of thermal analysis and calorimetry 60 (2000), S. 1081-1091 
    ISSN: 1572-8943
    Keywords: second law of thermodynamics ; temperature ; thermometry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract A brief outline of the history of development of the temperature concept in physics is given. Simultaneously, some persisting imperfections in the conceptual basis of classical thermodynamics closely related to the first and the second law of thermodynamics are discussed.
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  • 9
    ISSN: 1573-1561
    Keywords: Allelopathy ; autotoxicity ; activated charcoal ; cucumber ; Cucumis sativus L ; 2,4-dichlorobenzoic acid ; organic acids ; photoperiod ; temperature ; root exudates
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract In order to elucidate the effects of temperature and photoperiod on the quality and quantity of plant root exudates, a Japanese cucumber (Cucumis sativus, cv. Shougoin-Aonaga-Fushinari) was grown hydroponically in growth chambers under controlled temperature and photoperiod conditions with or without the addition of activated charcoal (AC) to the nutrient solutions. Fresh AC was used to trap the organic compounds exuded from cucumber roots every two weeks. Cucumber plants without AC were severely retarded in root growth and in the accumulation of dry matter, especially at high temperature and long photoperiod, compared to those with AC. The growth inhibitors, adsorbed on the AC or accumulated in the nutrient solution without AC, were extracted by organic solvents and analyzed by GC-MS. Benzoic acid and its derivatives, cinnamic acid derivatives, and fatty acids were identified. The rate of root exudation in vegetative and reproductive stages for some of these organic acids increased with the elevation of temperature and the elongation of photoperiod, and the mean rate was two or more times higher than the minimum exudation at low temperature with short photoperiod. Some of the identified compounds significantly inhibited the germination and/or root growth of lettuce and cucumber.
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  • 10
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    Environmental monitoring and assessment 64 (2000), S. 359-377 
    ISSN: 1573-2959
    Keywords: Tillamook ; watershed assessment ; North Coast ; Gene Conservation Area ; physical habitat ; benthic macroinvertebrates ; fish
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract A fine-grained statisticaly robust probability sample of stream segments is used to compare two small (20,000 hectare) subbasins of the Tillamook watershed, north coastal Oregon. The two subbasins are matched with respect to several variables [size coastal climates], but vary in terms of geology and consequently land use. A total of 67 wadeable + non-wadeable sizes were identified for sampling in the two subbasins (combined) over two field seasons from a sampling universe consisting of the River Reach File 3 (blue lines on 1:100,000 maps). Target variables include an extensive array of physical habitat endpoints, selected water chemistry endpoints, species composition, and relative abundance of both benthic macroinvertebrates and fish. Field protocols generally followed those of the U.S. EPA's Environmental Monitoring and Assessment Program (EMAP). Eleven fish species were encountered, a typically low number for coastal Oregon streams. Exploratory analysis using nonmetric multidimensional scaling revealed that 92.4% of the variation in the fish assemblages could be explained with two ordination axes. Environmental factors related to stream size and substate were the most correlated to these axes. Further, stream segments for the two subbasins tended to map in different areas of species space. Therefore, we also give unweighted probability distributions for several of the factors that heavily on these two axes by subbasins, as well as probability distributions for chemical endpoints. Results from the subset of sites sampled during the first year (21 wadeable sites) reveal: 1) differences between samples from the two subbasins relates to dream size and substrate composition that are consistent with known differences in geology and land use, 2) unexpectedly minor differences between samples from the two subbasins for stream temperature, canopy cover, and dissolved oxygen, 3) differences between samples from the two subbasins for total P, and total N, possibly related to land use, and 4) unexpected differences in samples from the two subbasins for conductivity, probably related to geological factors. Sample size for each subbasin is low and therefore our samples cannot be taken to necessarily characterize either subbasin. However, our findings are consistent with a comprehensive assessment that had been previously produced for one of the two subbasins. All field work was completed in 8 weeks 3-person field crew. We conclude that rapid assessment protocols, based on probability samples at this level of resolution, can be a cost-effective approach to watershed analysis. This approach should be seen as a complement to, rather than a replacement for, systematic surveys that produced finer scale, reach specific information on factors such as channel complexity and cover relevant to in-stream restoration planning.
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  • 11
    ISSN: 1573-3017
    Keywords: fish ; reproduction ; endocrine disruption ; xenobiotics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract The reproductive function of fish, which is very sensitive to the variations of environmental factors, appears also to be particularly vulnerable to the presence of xenobiotics in the aquatic medium. Many physiological processes can be impaired, from sexual differentiation to female and male gametogenesis, due to disruptions among complex neuro-endrocrine, endocrine or paracrine regulations. This paper describes the main regulation steps that are known or can be suspected to be disrupted by xenobiotics and gives some examples. The large interspecific diversity of reproductive strategies and the complexity of underlying mechanisms are particularly highlighted to draw attention to possible confusions between real endocrine disruptions and natural physiological variations.
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  • 12
    ISSN: 1573-4943
    Keywords: Ribonuclease A ; limited proteolysis ; temperature ; guanidine hydrochloride ; unfolding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Notes: Abstract Moderate temperatures or low concentrations of denaturants diminish the catalytic activity of some enzymes before spectroscopic methods indicate protein unfolding. To discriminate between possible reasons for the inactivation of ribonuclease A, we investigated the influence of temperature and guanidine hydrochloride on its proteolytic susceptibility to proteinase K by determining the proteolytic rate constants and fragment patterns. The results were related to changes of activity and spectroscopic properties of ribonuclease A. With thermal denaturation, the changes in activity and in the rate constants of proteolytic degradation coincide and occur slightly before the spectroscopically observable transition. In the case of guanidine hydrochloride-induced denaturation, however, proteolytic resistance of ribonuclease A initially increases accompanied by a drastic activity decrease far before unfolding of the protein is detected by spectroscopy or proteolysis. In addition to ionic effects, a tightening of the protein structure at low guanidine hydrochloride concentrations is suggested to be responsible for ribonuclease A inactivation.
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  • 13
    ISSN: 1608-3237
    Keywords: poly-β-hydroxybutyric acid ; Yersinia pseudotuberculosis ; Listeria monocytogenes ; temperature ; growth
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A comparative investigation of the intracellular content of poly-β-hydroxybutyric acid showed that Yersinia pseudotuberculosis strains accumulated, on the average, lower amounts of this reserve substance than Listeria monocytogenes strains. The intracellular pool of poly-β-hydroxybutyric acid was responsible for the growth of the bacteria at low temperatures (4–6°C) in the absence of any exogenous carbon and energy source.
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  • 14
    ISSN: 1608-3407
    Keywords: red microalgae ; collection of algae ; maintenance conditions ; darkness ; temperature ; chlorophyll fluorescence ; phycobiliproteins ; viability
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effects of light and temperature conditions on the maintenance of some red microalgae (order Porphyridiales) in collections were studied. Periodical subculturing on solid and liquid media was used for maintenance. Halophilic species P. aeruginosa, P. cruentum, and P. sordidum were lost in 2.5 months if kept in darkness. The viability of algae in the dim light slightly declined in 4–5 months and was species-dependent. The results were compared with earlier obtained data on mesophilic Chlorococcales. For the green algae, darkness proved to be the optimal condition, while their viability markedly decreased at light. It was suggested that this discrepancy was caused by the presence of phycobiliproteins in the cells of red algae. Dim light conditions are optimal for the synthesis of phycobiliproteins, which are rapidly destroyed in darkness, thus leading to cell death.
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  • 15
    ISSN: 1573-5125
    Keywords: diapause ; Diaptomus ; permanent environment ; photoperiod ; pond chemistry ; proximate cues ; temperature ; temporary environment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Field and laboratory studies were carried out between 1995 and 1997 on four populations of Diaptomus leptopus found in seasonally temporary, occasionally temporary, and a permanent environment to assess the relative importance of photoperiod and temperature regimes versus other proximate local cues in inducing diapause egg production. Patterns of diapausing and subitaneous egg production were determined by observation of individual females bearing clutches that were produced in the field. A laboratory common-garden experiment was performed to assess the effects of four different regimes of temperature and photoperiod on the induction of diapause. Patterns of diapausing egg production differed among ponds: diapause occurred early in the seasonally temporary environment and occurred rarely in the permanent environment. In the common-garden experiment, populations exhibited substantial changes in the onset of diapause when compared to patterns found under field conditions. These results provide indirect evidence that the different populations respond to available cues of environmental change in different ways in nature, or that environmental cues vary among habitats.
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  • 16
    ISSN: 1573-5125
    Keywords: body size ; chlorophyll concentration ; salinity ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The seasonal variation in length of the most abundant copepod species in the lagoon of Venice were compared and the relative influence of temperature, chlorophyll concentration and salinity examined. Temperature seems to be the primary factor influencing copepod body length in the lagoon of Venice, but the different species vary markedly in their response. Calanoid copepods (Acartia clausi, A. tonsa, Paracalanus parvus and Centropages ponticus) showed a more definite trend of size variation with temperature than the cyclopoids (Oithona nana and O. similis) and the harpacticoid Euterpina acutifrons. The size of the poecilostomatoids Oncaea media and O. subtilis was not affected by temperature, and was almost constant over time. Differences in the metabolic rates, longevity and specialization of calanoid, cyclopoid and harpacticoid copepods could explain their different responses to temperature.
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  • 17
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    Journal of applied phycology 12 (2000), S. 527-534 
    ISSN: 1573-5176
    Keywords: aquaculture ; fish ; hatchery ; microalgae ; molluscs ; phytoplankton ; post-larvae ; shrimp
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Algae are utilized diversely in aquaculture, but theirmain applications are related to nutrition. They areused in toto, as a sole component or as a foodadditive to supply basic nutrients, color the flesh ofsalmonids or for other biological activities. The needfor nutritional sources safer than traditional animalproducts has renewed interest in plants in general andalgae in particular. This report deals principallywith the nutritional role of microalgae inaquaculture.The larvae of molluscs, echinoderms andcrustaceans as well as the live prey of some fishlarvae feed on microalgae. Though attempts have beenmade to substitute inert particles for thesemicro-organisms which are difficult to produce,concentrate and store, only shrimp and live prey forfish will accept inert food, and only shrimp accept itfully. Several studies have confirmed that a live,multi-specific, low-bacteria microalgal biomassremains essential for shellfish hatcheries. Majoradvances are expected from new production systemdesigns and operations, from batch-run open tanks tomore sophisticated continuously run and closed loopreactors. Studies are underway to simplify hatcheryoperations by replacing biomass produced on-site withrun-times by that produced and preserved elsewhere.Although still promising, they have not given rise, sofar, to any application for molluscs. Otherapplications of microalgae in aquaculture, from greenwater to making salmon flesh pinker, are examined.Whether produced on or off-site, there remains thequestion of cost effectiveness of microalgalproduction systems. This can only be achieved bysubstantial upscaling and improved quality control.
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  • 18
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    Aquatic ecology 34 (2000), S. 227-242 
    ISSN: 1573-5125
    Keywords: cladocerans ; demography ; Microcystis aeruginosa ; rotifers ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The response of selected rotifers and cladocerans to Microcystis aeruginosa, offered as colonies and single cells, was compared to that on a diet of Chlorella vulgaris using the life table demography approach. The test zooplankton species were Simocephalus vetulus, Daphnia carinata, Moina macrocopa, Scapholeberis kingi, Ceriodaphnia cornuta, Brachionus calyciflorus and Hexarthra mira. To detect the development of resistance to toxins from Microcystis in zooplankton, in addition to the laboratory cultured strain of Ceriodaphnia cornuta, another strain of the same species was also used (designated as C. cornuta 2, this was collected from a pond containing Microcystis and cultured in the laboratory on Chlorella for a few weeks prior to experimentation). Experiments were conducted at 20 °C and 30 °C. Survivorship was high on Chlorella in most species but low on diets of Microcystis. Except for C. cornuta 2, S. kingi and S. vetulus, all other test species were adversely affected by Microcystis. The ability to utilise Microcystis improved at 30 °C in M. macrocopa, D. carinata and H. mira. The longest mean lifespan was recorded for C. cornuta 2 (25.3 ± 4.86 d) and the lowest for B. calyciflorus (0.58 ± 0.05 d). The highest net reproductive rate was observed for C. cornuta 1 (44.9 ± 4.88) and the longest generation time of 26.6 ± 2.13 d for S. vetulus. Among the cladocerans that showed positive values of population growth rate (r), M. macrocopa had the highest of 0.96 ± 0.04 per day.
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  • 19
    ISSN: 1573-5176
    Keywords: desiccation ; growth ; growth model ; inorganic carbon ; nutrients ; photoperiod ; photosynthesis ; pigments ; Porphyra linearis ; PPF ; respiration ; temperature ; water velocity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of environmental parameters on the growthof Porphyra linearis gametophytes was examinedunder controlled conditions, and related to themultilinear regression growth model recently developedfor this seaweed under coastal conditions in theeastern Mediterranean. Growth chambers, a gradienttable, special culture devices and analytical methodswere combined for this culture study.The major factors significantly controlling thegrowth rate of the P. linearis gametophytein glass dishes were: photoperiod, temperature, agein culture, photosynthetic photon flux (PPF), salinityand water dynamics. Maximal growth occurred underdaylength of 12 h, medium temperature (15–20 °C), low PPF (70–140 μmol photon m-2s-1), ambient salinity (30–40 ppt), 1–3 h ofdaily air exposure, and water velocity of 4 cm s-1.Photosynthesis and respiration rates weredominantly affected by daylength and temperature,while the concentration of pigments was dominantlyaffected by PPF and temperature.These conditions correspond well to the optimalnatural growth environment of this local species andare in agreement with the optimum estimated throughthe recently developed outdoor mathematical growthmodel.
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  • 20
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    Aquaculture international 8 (2000), S. 513-530 
    ISSN: 1573-143X
    Keywords: great scallop (Pecten maximus) ; hatchery ; metamorphosis ; seawater flow ; spat development ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Variations in growth and survival of hatchery-reared post-metamorphicjuveniles of great scallop Pecten maximus prompted anexamination of settlement and postlarval development. The effects ofseawater flow and temperature on great scallop metamorphosis andpostlarvae were studied over a 4–5 week period. In allexperiments, and regardless of environmental conditions, great scallopmetamorphosed after a 2–3 week period with values of 35 to70%. Subsequently, spat numbers increased slightly. Spatmortality generally occurred from the third week onward and reachedlevels as high as 30% by the fifth week under standardconditions. At 20 °C, however, 60% mortality levels wererecorded. Differences in spat growth rate, ranging from 37 to 45 μmday−1, were noticed at different seawater flow ratesbut no clear tendency could be discerned. Temperature affected spatgrowth with an increase in size from 24 μm day−1 at15 °C to 35 μm day−1 at 18 °C. Conversely,growth was suppressed at 20 °C (14 μm day−1).For optimal metamorphosis and postlarval development in great scallop, aseawater flow of 4.3 L h−1 per sieve and a temperatureof 15 °C are recommended.
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  • 21
    ISSN: 1573-5184
    Keywords: beaver ; Castor fiber ; Castor canadensis ; fish ; salmonids ; stream communities ; stream habitats
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The Eurasian and North American beavers aresimilar in their ecological requirements, andrequire water deep enough to cover the entranceto their lodge or burrow. A food cache isoften built next to the lodge or burrow, exceptin some southern areas. On small streams (upto fourth order) dams are frequently built tocreate an impoundment, generally on lowgradient streams, although at high populationdensities dams may be built on steeper gradientstreams. On large rivers or in lakes, simply alodge with its food cache may be built. Thebeaver is a keystone riparian species in thatthe landscape can be considerably altered byits activities and a new ecosystem created. The stream above a dam changes from lotic tolentic conditions. There are hydrological,temperature and chemical changes, depending ontypes of dams and locations. Although theinvertebrates may be fewer per unit area, totalnumber of organisms increases, and diversityincreases as the pond ages. In cool, smallorder streams, the impoundments provide betterhabitat for large trout, possibly creatingangling opportunities. However, at sites wherewater temperatures rise above their optimumpreferenda, salmonids may be replaced by otherspecies, such as cyprinids, catostomids,percids or centrarchids. As the habitat isaltered, interactions amongst co-habiting species may change. For example, brown troutor brook trout (charr) may become dominant overAtlantic salmon. In warm water streams theremay be a shift from faster water dwellers topond dwellers. Larger bodied fish, such ascentrarchids and esocids may displace smallerbodied fish such as cyprinids, providing betterangling. Refugia from high or low water flows,low oxygen or high temperatures, may beprovided in adverse conditions in winter orsummer. However, in some cases dams areobstructions to upstream migration, andsediment may be deposited in former spawningareas. The practicality and benefits ofintroducing or restoring beaver populationswill vary according to location, and should beconsidered in conjunction with a managementplan to control their densities.
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  • 22
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    Evolutionary ecology 14 (2000), S. 627-643 
    ISSN: 1573-8477
    Keywords: adaptation ; Bergmann's rule ; clutch size ; egg size ; physiological constraint ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Organisms and parts of an organism like eggs or individual cells developing in colder environments tend to grow bigger. A unifying explanation for this Bergmann's rule extended to ectotherms has not been found, and whether this is an adaptive response or a physiological constraint is debated. The dependence of egg and clutch size on the mother's temperature environment were investigated in the yellow dung fly Scathophaga stercoraria. Smaller eggs were laid at warmer temperatures in the field and the laboratory, where possible confounding variables were controlled for. As clutch size at the same time was unaffected by temperature, this effect was not due to a trade-off between egg size and number. Temperature-dependent egg sizes even persisted within individuals: when females were transferred to a cooler (warmer) environment, they laid third-clutch eggs that were larger (smaller) than their first-clutch eggs. The fitness consequences of these temperature-mediated egg sizes were further investigated in two laboratory experiments. Neither egg and pre-adult survivorship nor larval growth rate were maximized, nor was development time minimized, at the ambient temperature corresponding to the mother's temperature environment. This does not support the beneficial acclimation hypothesis. Instead, this study yielded some, but by no means conclusive indications of best performance by offspring from eggs laid at intermediate temperatures, weakly supporting the optimal temperature hypothesis. In one experiment the smaller eggs laid at 24°C had reduced survivorship at all ambient temperatures tested. Smaller eggs thus generally performed poorly. The most parsimonious interpretation of these results is that temperature-mediated variation in egg size is a maternal physiological response (perhaps even a constraint) of unclear adaptive value.
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  • 23
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    Wetlands ecology and management 8 (2000), S. 37-51 
    ISSN: 1572-9834
    Keywords: Africa ; estuary ; fish ; juvenile ; mangrove ; nursery
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The mangrove system of Sine Saloum in Senegal is characterized by the lack of permanent river flow, in the context of the Sahelian drought which began in the 70s. The main environmental consequence is that Sine Saloum has become a so called ‘reversed estuary’ with salinity increasing upstream and reaching 100‰ and more, with a mean salinity between 45–50‰. A three‐year survey of the juvenile fish community was undertaken with the aim of verifying whether or not this environment is still suitable as a nursery area for exploited fish populations. The main sampling gear used were small fyke‐nets in addition to gill nets and a limited rotenone sampling. One of the six mangrove stations included in the survey exhibited a relatively high species diversity. This station is the only one where salinity may reach levels as low as 25‰ at the end of the rainy season, although salinity is much higher in the upstream region near this station. Such a low salinity is likely due to an underground freshwater connection or underwater springs. These observations highlight the relative importance of estuaries and mangroves in the nursery function.
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  • 24
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    Powder metallurgy and metal ceramics 39 (2000), S. 599-602 
    ISSN: 1573-9066
    Keywords: solid-phase reaction ; solubility ; temperature ; cobalt silicides ; boron
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract The system Co ― Si ― B is promising for development of new soft magnetic materials. We studied the effect of boron on the structure and composition of cobalt silicides during solid-phase reaction of powders of the starting components under a 3·10−3 Pa vacuum in the temperature range 200°C to 1300°C for boron content from 0.5 mass% to 7.7 mass% by differential thermal analysis, metallographic analysis, x-ray analysis, and electron-probe microanalysis. We established that boron is not soluble in cobalt silicides. Formation of ternary chemical compounds is typical for the Co ― Si ― B system.
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  • 25
    ISSN: 1573-5036
    Keywords: Arctic ; Cerastium alpinum ; nitrogen source ; nitrogen-uptake ; organic N ; polar-desert plants ; Saxifraga caespitosa ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Polar-desert plants experience low average air temperatures during their short growing season (4–8 °C mean July temperature). In addition, low availability of inorganic nitrogen in the soil may also limit plant growth. Our goals were to elucidate which N sources can be acquired by polar-desert plants, and how growth and N-uptake are affected by low growth temperatures. We compared rates of N-uptake and increases in mass and leaf area of two polar-desert species (Cerastium alpinum L. and Saxifraga caespitosa L.) over a period of 3 weeks when grown at two temperatures (6 °C vs. 15 °C) and supplied with either glycine, NH4 + or NO3 −. At 15 °C, plants at least doubled their leaf area, whereas there was no change in leaf area at 6 °C. Measured mean N-uptake rates varied between 0.5 nmol g−1 root DM s−1 on glycine at 15 °C and 7.5 nmol g−1 root DM s−1 on NH4 + at 15 °C. Uptake rates based upon increases in mass and tissue N concentrations showed that plants had a lower N-uptake rate at 6 °C, regardless of N source or species. We conclude that these polar-desert plants can use all three N sources to increase their leaf area and support flowering when grown at 15 °C. Based upon short-term (8 h) uptake experiments, we also conclude that the short-term capacity to take up inorganic or organic N is not reduced by low temperature (6 °C). However, net N-uptake integrated over a three-week period is severely reduced at 6 °C.
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  • 26
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    Hydrobiologia 419 (2000), S. 141-148 
    ISSN: 1573-5117
    Keywords: Crustacea ; Ostracoda ; Krithe praetexta praetexta ; life cycle ; ontogeny ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The effect of temperature on growth rate, shell size and shell shape in Krithe praetexta praetexta (Sars) was studied in four thermocultures. From July 1995 to June 1996, the cultures were kept in a continuously flowing open system pumping water from the intermediate watermass of the Gullmarn fjord, west coast of Sweden. Three cultures were kept at constant temperatures of 5, 10 and 14 °C, respectively. The fourth (reference) culture largely followed the natural variation in temperature. At the termination of the experiment, all living ostracods from a 125 μm sieve were sampled from the cultures. Population age structures were analysed for the various thermocultures of K. praetexta praetexta. These were more shifted towards later ontogenetic stages with higher temperature, i.e. the ontogenetic development was more rapid in the warmer cultures. An alternative explanation is due to diapause causing cohorts to accumulate in some ontogenetic stages only when the temperature is constant. The differences in shell size of K. praetexta praetexta among the thermoconstant cultures were not statistically significant.
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  • 27
    ISSN: 1573-5117
    Keywords: Chirocephalus ; metabolic potential ; intermittent lake ; ecology ; physiology ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Electron transport system (ETS) activity was measured in Chirocephalus croaticus from the intermittent lake, Petelinjsko Jezero. The ETS activities were measured at 5, 10, 15, 20, and 25 °C, and were studied separately in juveniles, females and males. Juveniles had significantly higher activity than adults at a standard temperature of 20 °C. The mass-specific ETS activity decreased with increasing size of the animals; the value b was 0.787. Respiration rates (R) were determined at 20 °C and the ratio ETS/R (±standard deviation) for C. croaticus was 1.43±0.46 (n=38). ETS activity increased with temperature. Females had higher Q10 than males in higher temperature range (t-test; t=2.50; d.f.=8; p〈0.05). Activation energy Ea was higher for females than males (t-test; t=2.35; d.f.=8; p〈0.05). Females exhibited lower ETS activity than males over the lower temperature range, but their ETS could function more efficient at higher temperature.
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  • 28
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    Environmental biology of fishes 57 (2000), S. 113-115 
    ISSN: 1573-5133
    Keywords: photoperiod ; temperature ; spawning ; larva rearing ; larva food
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Adult fish of a freshwater population of the Iberian endangered cyprinodontid Aphanius iberus, were induced to reproduce at salinities of 5, 15, 30, 45 and 60 ppt. For each salinity five 30 l aquaria were used, each one including a male and two females. Maturity and spawning outside the natural season, were obtained at conditions of 22 to 28°C and a photoperiod of 14L:10D. The larvae were fed with rotifers Brachionus plicatilis and Synchaeta cecilia valentina. Experiment lasted 40 days. The first spawning occurred on the 17th day at 45 ppt of salinity and the first embryos hatched on the 34th day at 5 and 15 ppt salinity. The final average number of larvae per aquarium ranged from 5.2 (45 ppt salinity) to 10.8 (15 ppt salinity). No significant differences were found between the average values at different salinities (p〈0.01).
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  • 29
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    Environmental biology of fishes 57 (2000), S. 353-361 
    ISSN: 1573-5133
    Keywords: exotic ; fish ; Indian River Lagoon
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract I investigated the reproductive biology of introduced blackchin tilapia, Sarotherodon melanotheron, over a twelve month period within a seasonally impounded mangrove ecosystem in east-central Florida, and compared the data to published accounts from native African habitats. Eggs and free embryos were collected from the buccal cavity of adult males from April to October indicating a protracted spawning season, however gonadosomatic indices suggested that most spawning occurred in April and May. The timing of reproduction was adjusted to occur immediately before temperatures reached optimal levels for juvenile growth and when increasing rainfall produced abundant food and shelter for the young. Temperatures below 24°C inhibited reproduction from November to March. The overall sex ratio was essentially 1:1. The minimum and maximum size at maturity were much larger in this study than those reported from Africa; the smallest mature male was 128 mm standard length and 0.47 of the maximum size, while the smallest mature female was 145 mm standard length and 0.54 the maximum size. However, because of the difficulty in aging this species and a lack of published information, it could not be determined whether the population in this study possessed a relatively early or delayed maturation compared to African populations.
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  • 30
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    Environmental biology of fishes 58 (2000), S. 109-112 
    ISSN: 1573-5133
    Keywords: daily food intake ; food consumption ; fish
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Daily food intake of adult burbot, Lota lota, fed on vendace, Coregonus albula, were estimated experimentally at four different water temperatures (2.4, 5.1, 10.8 and 23.4°C). Mean daily food intake (MDI; g d−1) and relative daily food intake (RDI; g g−1 d−1) increased with temperature from 2.4 to 10.8°C and decreased at 23.4°C. Temperatures of maximum daily food intake values were 13.6°C for MDI and 14.4°C for RDI. No correlation between food intake values and burbot weight was observed. RDI values were used to estimate annual food consumption of burbot population. Annual food consumption estimates were 9.7 kg ha−1 and 24.3 kg ha−1 when burbot biomass was 2.0 or 5.0 kg ha−1, respectively.
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  • 31
    ISSN: 1573-5133
    Keywords: ultraviolet ; UV-blocker ; fish ; MAA ; lens ; cornea
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The visual ecology of fishes places changing demands on their visual system during development. Study of changes in the eye can suggest possible changes in behavioral ecology. The spectral transmission of the pre-retinal ocular media controls the wavelength of light that reaches the retina and is a simply measured indication of their potential visual capabilities. Dascyllus albisella is a coral reef planktivore known to have UV-sensitive retinal cone cells. UV vision probably aids in detection of zooplankton. As a juvenile it is very closely associated with branching coral heads or, more rarely, sea anemones. As it matures, it ventures farther from its coral, above the reef, and eventually assumes a more vagile life style, moving farther and more frequently afield. Their eyes contain short-wavelength blocking compounds in the lens, cornea and humors. As they age, both the lens and the cornea accumulate blocking compounds that increase the 50% transmission cutoff of the whole eye from ca. 330 nm in 2–3 cm juveniles to ca. 360 nm in the largest adults. The cornea increases its cutoff wavelength faster than the lens and becomes the primary filter in large adults. The cutoff of the aqueous and vitreous humors combined does not change with size. The slope of the transmission cutoff curve increases with the size of the fish. The increased blocking of UV radiation is likely not an adaptation to protect the eye from short-wavelength induced damage. Instead it probably reduces the image degradation effects of short-wavelength light in the largest eyes and still allows sufficient penetration of UV radiation to permit functional UV vision.
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  • 32
    ISSN: 1573-5133
    Keywords: deep pools ; young salmonids ; habitat use ; habitat availability ; temporal variation ; temperature ; winter
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract There was a pronounced decline in activity of young pool-dwelling Atlantic salmon, Salmo salar, and brown trout, Salmo trutta, as the water temperatures dropped in the autumn and early winter, and the fish switched from a predominantly diurnal towards a nocturnal activity pattern. Such a switch in activity pattern has previously been observed in young brown trout, but the present study is the first documentation for juvenile Atlantic salmon under natural conditions. Juvenile fish fed actively even when water temperatures were below 0°C, although foraging behaviour at near-freezing temperatures was recorded exclusively during night surveys. This indicates that other proximate factors, in addition to water temperature, affect the activity of young salmon and trout in rivers. Trout kept feeding positions significantly higher above bottom than salmon in August and September, but both species reduced the height above bottom at the onset of winter, possibly due to reduced swimming performance and lowered food availability in the upper part of the water column.
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  • 33
    ISSN: 1573-5117
    Keywords: México ; lake ; fish ; biotic integrity ; IBI ; ecosystem health
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The lakes of central Mexico have great cultural, economic, and biological value, but they are being degraded at an accelerating rate. We employed historical data on fish communities from 19 of these lakes and case studies of community responses to environmental degradation from four of the best-studied, Xochimilco, Cuitzeo, Chapala, and Pátzcuaro, to construct a preliminary index of biotic integrity (IBI). This IBI was designed to be an easily applied method for assessing lake ecosystem health and evaluating restoration efforts. The IBI had 10 metrics: number of total native species, number of common native species, number of native Goodeidae species, number of native Chirostoma species, number of native sensitive species, percent of biomass as tolerant species, percent of biomass as exotic species, percent of biomass as native carnivorous species, maximum standard length of native species, and percent of exotic invertebrate parasite species on or in native fishes. Initial applications of the index showed promise, accurately ranking the relative degradation of the four case-study lakes. Further tests of the index are warranted, and more data are needed to standardize sampling procedures, improve species classifications, and refine metric scoring criteria.
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  • 34
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    Hydrobiologia 441 (2000), S. 55-62 
    ISSN: 1573-5117
    Keywords: climate change ; temperature ; mayflies ; Cloeon dipterum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Populations of the mayfly Cloeon dipterum from 48 ponds (3000 l fibre-glass tanks of 1 m depth) were monitored over the course of 1 year. To simulate possible patterns of climatic change, the ponds were subject to three temperature treatments: continuous heating to 3 °C above ambient; heating to 3 °C above ambient during the summer only; and no heating. Further experimental complexity included enhanced nutrient input into the ponds and the presence or absence of fish, giving a factorial combination of 3 temperature regimes × 2 nutrient levels × presence/absence of fish predation. Few nymphs were found in the presence of fish. Where fish were absent, the temperature treatments did not significantly affect nymph abundances, and only marginally influenced mean nymph body-lengths. In contrast, the nutrient treatment had significant effects on both nymph abundance and size, with greater numbers of generally larger nymphs occurring in those fish-free ponds receiving additional nutrients. Adult emergence began earlier in the year from the heated ponds, particularly those ponds receiving additional nutrients. Adult body-length differed between temperature treatments, but consistent patterns were difficult to ascertain because of interactions with nutrient treatment and seasonal effects. Our results show that during the short term at least, elevated temperature as a simulation of climate change does not have an overwhelming influence on either mayfly abundance or size. The influence of temperature is subtle and subject to complex interaction with other habitat variables. We therefore suggest that the direct consequences of small changes in temperature will likely be of little significance to C. dipterum, relative to indirect effects operating through interactions with predation and nutrient input.
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  • 35
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    Hydrobiologia 422-423 (2000), S. 271-278 
    ISSN: 1573-5117
    Keywords: biodiversity ; bioenergetics ; bioindicators ; disturbance ; fish ; floodplains ; ecological integrity ; monitoring ; restoration ; river engineering
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Fish communities in large rivers are characterized by a high diversity, which reflects the structural diversity and habitat richness of inshore zones and connected floodplains. The connectivity of the different habitat elements in a broad spatio-temporal context, i.e., at various scales from catchment to microhabitat – and their nestedness – define the fitness of fish species both on the individual level (e.g., growth performances) and population level (i.e., population structure, mortality, etc.). Relevant spatial scales can be the whole river course in the case of some anadromous migrators or the availability of complementary microhabitat elements, e.g., during the early ontogeny of a species. The significance of connectivity at various scales from whole river to local reach have to be evaluated based on the requirements, reaction norms, and ecological flexibility of individual species. Integrity has to be evaluated in several respects: (1) with regard to population genetics over extensive biogeographicareas and in long time scales; (2) with regard to supplementary habitats in the course of the life cycle of individual species with ontogenetic habitat shifts and specific requirements during the reproductive phase; (3) with regard to longitudinal and lateral transport and exchange processes determining local habitat conditions and the food supply for fish. Due to these dependencies the status and condition of the fish fauna is a critical sensor of integrity at different scales and thus a good monitoring tool especially with regard to river engineering. The structure of the fish population – in particular the relative abundance of specifically adapted floodplain species – provides information on the overall conditions. For a more detailed evaluation the local distribution pattern of the fish fauna, population structure, seasonality, and growth performances of the early life history stages can indicate the quality and extent of connectedness at a finer scale and can be used to define management and engineering criteria.
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  • 36
    ISSN: 1573-5117
    Keywords: Meganyctiphanes norvegica ; respiration ; temperature ; vertical migration ; Kattegat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Alkor-Deep (140 m), which forms part of a depression system in the northern Kattegat channel east of the island of Læsø (Denmark), is the location of a self sustaining population of Northern krill, Meganyctiphanes norvegica (Euphausiacea). This population is exposed to one of the most pronounced thermal gradients within the distributional range of this pelagic crustacean. During summer, the temperature of the water column ranges between 4 and 6 in the deep to 16 °C near the surface which results in the krill being exposed to temperature differences of 8–10 °C during diel vertical migration. Oxygen consumption rates were used to investigate the physiological adaptation of the animal to such gradients in temperature. The rates were found to increase exponentially from 31 μmol O2 h-1 gdw -1 at 4 °C to 72 μmol O2 h-1 gdw -1 at 16 °C, giving a Q 10-value of 2.0, and indicating that physiological adaptation to varying thermal conditions does not take place. Behavioural adaptations are discussed which may help the krill to cope with large temperature gradients in their environment.
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  • 37
    ISSN: 1573-5117
    Keywords: Cercopagis ; invasion ; Baltic Sea ; depth ; temperature ; salinity ; morphological variations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The onychopod cladoceran Cercopagis that recently invaded the Baltic Sea is reported from new zones of the northern Baltic proper. Because of successful survival and an expanding distribution range, the addition of Cercopagis to the Baltic fauna is considered to be permanent. What has previously been cited as Cercopagis pengoi encompasses the morphology of several other species, subspecies and forms. Either a number of morphologically similar species is present, or there is a number of spurious species in Cercopagis. The last hypothesis is favoured. The spatial distribution pattern of Cercopagis, as well as that of total zooplankton, was correlated with depth. Deep (〉100 m) and shallow (〈10 m) stations had significantly lower abundance than stations of intermediate depth (〈100 m). An overview of the distribution of C. pengoi group in fresh and brackish waters suggests a high tolerance to environmental factors, but with differences among taxa. Due to this ecological flexibility, the colonization of the Baltic is not unexpected. Increasing salinity may restrict dispersal of cercopagids to the southern areas of the Baltic and to the North Sea, but inland lakes (e.g. in Sweden) present an ecological profile suitable for colonization.
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  • 38
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    Hydrobiologia 431 (2000), S. 175-184 
    ISSN: 1573-5117
    Keywords: Dreissena ; lake stratification ; water clarity ; temperature ; oxygen ; metalimnion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Colonization and proliferation of zebra mussel (Dreissena polymorpha) population in Hargus lake, a small thermally stratified reservoir in Ohio, U.S.A., caused a significant increase in water clarity and a remarkable decrease in phytoplankton biomass during the period from 1993 to 1995. Increased light penetration and reduced organic matter loading to the meta-and hypolimnion were reflected in the lake stratification patterns, particularly in the temperature and oxygen profiles in the metalimnion. The meta- and hypolimnetic water temperature increased significantly over three years, irrespective of variation in surface water temperature. The epilimnion depth (mixing depth) increased by about the same magnitude as did the average Secchi depth. However, the total heat content of the lake did not show a consistent trend to increasing zebra mussel abundance, as it was largely influenced by the temperature of the large water volumes near the surface, which were in turn affected by weather conditions. Concurrent with the thermal structure change, the dissolved oxygen structure also changed over three years, though to a lesser extent. The changes in oxygen stratification pattern were reflected by increased oxygen concentrations in the metalimnion and a lowered depth of 3 mg l−1 DO isopleth. These observed changes were likely attributed to increased water mixing depth, metalimnion photosynthesis and reduced oxygen consumption by organic matter. With increased epilimnion thickness and improved oxygen conditions in the metalimnion, the habitable space for aquatic macro-organisms (including fish) expanded substantially. Our results suggest that the indirect impacts of zebra mussels on small lake stratification patterns may have much broader implications than do the direct trophic interactions to the whole ecosystem.
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  • 39
    ISSN: 1573-5117
    Keywords: nutrient flux ; nitrogen ; phosphate ; tidal flats ; temperature ; geographical comparison
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract During an annual cycle, flux rates of oxygen, nitrate, nitrite, ammonium, phosphate and silicate were measured in light and dark bell jars at three sites in Ria Formosa (Algarve, Portugal) enclosing either a natural macrophytic community (macroalgae on sand or mud, a seagrass bed of Zostera noltii) or bare sediments. The results are compared with a preceeding study in which the same bell jar technique has been applied in the Sylt-Rømø Bay of the northern Wadden Sea. Nitrate flux was mainly directed from the water column to the benthic communities in Ria Formosa, as well as in the Sylt-Rømø Bay. However, nitrate uptake was higher in the northern, more eutrophic study area. In Ria Formosa, nutrient concentrations were lower than in the Sylt-Rømø Bay possibly due to strong water exchange with Atlantic waters. High temperatures and strong insolation had a greater impact on nitrate fluxes in Ria Formosa than in the Sylt-Rømø Bay. Bioturbating macrofauna increased ammonium efflux in the Sylt- Rømø Bay while this effect was not as pronounced in the Ria Formosa study sites. Benthic phosphate uptake dominated in the Ria Formosa and was correlated to initial phosphate concentrations in incoming waters. At both study sites, oxygen and nutrient fluxes were correlated with temperature. Additionally, flux rates were strongly influenced by biotic components and levels of eutrophication. A literature survey showed that mainly in temperate regions, material fluxes increase with temperature, whereas in warmer areas, ammonium and phosphate fluxes between sediment and water were generally lower.
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  • 40
    ISSN: 1573-5133
    Keywords: seasonallity ; inter annual variations ; reproductive style ; fish ; cichlid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Fecundity and oocyte size of Oreochromis niloticus females were studied over a period of two annual cycles in six small agropastoral and three large hydroelectric reservoirs of Côte d'Ivoire. Important differences in fecundity and oocyte size were observed among populations and within the same population between successive years. An inverse correlation was found between size and number of oocytes produced by females. This inverse relationship occurred for a constant spawn weight during the first year of study, but not during the second year. Monthly mean residuals of regressions between fecundity and female body weight have shown a seasonal variation in fecundity. The peak of fecundity corresponded with the maximum resource availability and the flooding eminence, which may have a great impact on parents and offspring fitness.
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  • 41
    ISSN: 1573-5133
    Keywords: infrasound source ; acoustic fence ; fish barrier ; fish passage ; migration ; fish
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In an attempt to develop an efficient acoustic fish fence, we have designed an infrasound source able to generate large nearfield particle acceleration. The source generates water movements by means of two symmetrical pistons in an air-filled cylinder with 21 cm bore. The pistons are driven by eccentric coupling to an electric motor, with 5 cm p.p. amplitude. The piston movements are 180° out of phase. The piston reaction forces are thus opposed, leading to vibration free operation. The submergible infrasound source is operated freely suspended in the water mass. The emitted sound frequency is 11.8 Hz. The particle acceleration is about 0.01 m s−2 at a distance of 3 m, corresponding to the threshold intensity for deterring effects of infrasound on Atlantic salmon smolts. The sound source was employed to test the effect of intense infrasound on migrating European silver eels. Fish confined in a tank displayed startle behaviour and prolonged stress reactions, telemetrically monitored as tachycardia, in response to intense infrasound. The field tests were carried out in the River Imsa. A trap that catches all the descending eels is installed near the river mouth. The trap was separated in four equal sections. During the periods with infrasound exposure, the proportion of silver eels entering the section closest to the sound source was reduced to 43% of the control value. In the section closest to the opposite river bank, infrasound increased the proportion of trapped eels to 144% of the control values. This shift of the migrating eels away from the infrasound source was highly significant.
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  • 42
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    Environmental biology of fishes 57 (2000), S. 443-449 
    ISSN: 1573-5133
    Keywords: parental care ; feeding behaviour ; evolution ; trade-off ; individual differences ; fish
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The function of the fin digging behaviour in increasing food availability for the offspring was analysed in the convict cichlid, Cichlasoma (Archocentrus) nigrofasciatum. Consistent individual differences in the frequency of fin digging were found in the parental fish. Examination of the gastrointestinal tract of young revealed that higher frequency of parental fin digging was associated with higher consumption of large and more profitable prey (Diptera larvae), which inhabited deep horizons of the bottom substrate and possibly were difficult to access without parental assistance. Thus, parental fin digging was initially associated with a significant increase of the offspring growth rate. However, at later brood intervals, when parental care ceased, the young of the high-digging parents were characterised by a poorer consumption of small larvae that were most accessible for them without parental aid and represented an increasingly more important component of their ration than large larvae. Offspring of the low-digging parents, on the other hand, presumably as a result of their individual experience, showed a considerably better consumption of small larvae, increasing their growth rate. As a consequence, prior parental fin digging did not affect the offspring body size after independence. Thus, there exist pronounced individual differences and alternative parental styles in the convict cichlid.
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  • 43
    ISSN: 1573-5133
    Keywords: exchange area ; secondary lamellae ; fish ; gas exchange ; respiratory system ; functional morphology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In a previous study we showed that many of the morphological features of the respiratory system of GH (growth hormone) transgenic Atlantic salmon are greater than similarly sized control salmon. Here we show that the manifestation of GH transgene is similar in two different lines of GH transgenic Pacific coho salmon, but that it is very different from that in the GH transgenic Atlantic salmon. The GH transgenic Pacific coho salmon do not have a larger gill surface area than similarly sized control fish.
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  • 44
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    Environmental biology of fishes 58 (2000), S. 145-156 
    ISSN: 1573-5133
    Keywords: salt marsh ; fish ; estuary ; fish distribution ; Zostera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The structure and functioning of salt marsh fish communities in the overall ecology of southern African estuaries is poorly understood. This study compares the ichthyofauna associated with a salt marsh creek and eelgrass bed in an attempt to evaluate the relative importance of these habitats to fishes. Taylor's salt marsh creek and adjacent eelgrass bed in the Kariega Estuary were sampled twice per season between the winter of 1992 and the autumn of 1994. The average density and standing stock of fishes were found to be considerably higher in the eelgrass bed than in the intertidal creek. Both habitats had similar fish diversities but were dominated by different taxa, the most notable of which was the dominance of mugilids in the creek and their scarcity in the eelgrass. Taylor's intertidal creek and adjacent eelgrass beds were dominated by juvenile fish, with both habitats functioning as nursery areas for juvenile fish, albeit for totally different ichthyofaunal communities. The similar fish diversities but lower abundances in the intertidal creek compared to the eelgrass beds are in contrast to similar North American studies, and refute the hypothesis that intertidal salt marsh creeks have higher fish densities but lower diversities than eelgrass beds.
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  • 45
    ISSN: 1573-5133
    Keywords: diadromy ; migration ; otolith microchemistry ; fish
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Anadromous and resident forms of pond smelt, Hypomesus nipponensis, were found to occur in sympatry in Lake Ogawara, Japan. Profiles of Sr: Ca ratios from individuals could be grouped to two patterns (1) a ‘resident’ pattern with low Sr: Ca ratios from core to edge and (2) an ‘anadromous’ pattern with relatively low Sr: Ca ratios near the core with abrupt increases in ratios at a location approximately 0.3 mm from the core. Spawners smaller than 60 mm standard length (SL) were resident, between 60 to 80 mm were mixed resident and anadromous, and larger than 80 mm were anadromous. Anadromous individuals first migrated after 40 to 82 days from hatching (mean±sd, 59.1±13.5 d) and 14.6 to 30.9 mm SL (22.2±5.3 mm). There was no difference in SL between resident and anadromous individuals during age at first migration, suggesting that size may not be the mechanism for divergence of alternative life history styles.
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  • 46
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    Environmental biology of fishes 58 (2000), S. 455-460 
    ISSN: 1573-5133
    Keywords: aquaculture ; Colosoma mitrei ; fish ; overwintering ; Pantanal
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Pacu, Piaractus mesopotamicus (=Colossoma mitrei), is a South American warm water fish species found in the temperature range of 15–35°C. The culture of a warm water species in temperate regions demands knowledge on its temperature requirements. Pacu introduction into the Israeli fish culture system is being considered. Temperature range in the region is 8–33°C, thus the minimum winter water temperatures might be a limiting factor. To determine what is the minimum temperature pacu would tolerate, and hence which overwintering operations in warm-temperate regions are required for this warm water species, low temperature tolerance tests in the laboratory and observations in the field were carried out. Laboratory experiments reducing temperature by 1–3°C per day were carried out with fish of 150–200 g, about the size pacu reach after one culture season. The field observations compared survival of two-year-old pacu of 1.3 kg mean weight overwintered in outdoors and in greenhouse ponds. For one-year-old fish 7.5°C was found to be the lower temperature tolerance limit. Two-year-old fish withstand short exposures to this temperature rather well and their lower tolerance limit might be lower. This indicates that in warm-temperate regions pacu should survive in outdoors ponds. In this case some loss of weight should be expected, and suspension of feeding when temperature drops below 16–18°C is recommended to avoid wasting feed that the fish will not consume anyway. To be in the safe side, inflow of the warmest available water into the ponds is recommended if maximum water temperature drops to 10°C or below. Overwintering in greenhouses or other heated facilities would be recommended if an exceptionally cold winter is expected and for regions with lower winter minimum temperatures.
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  • 47
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    Environmental biology of fishes 59 (2000), S. 91-97 
    ISSN: 1573-5133
    Keywords: age ; growth ; reproduction ; mortality ; fish
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Atherina presbyter is a common fish off the Canary Islands. Age, growth, reproduction, and mortality of the species are studied based on sampling carried out from July 1995 to June 1996. The parameters of the total length–total weight relationship are: a=0.004521, and b=3.0771. Otoliths age readings indicate that the sampled population consists of four age groups (0–III years). The von Bertalanffy growth parameters for all individuals are: L∞=122 mm total length, k=0.79 year−1, and t0=−0.21 years. Individuals grow quickly in their immature first year, attaining approximately 60% of their maximum length. After the first year, the annual growth rate drops rapidly, because the energy is probably diverted to reproduction. It is a gonochoristic species with no evidence of sexual dimorphism. The gonad is present as a single diffuse testis in males and as a single discrete ovary in females. The overall ratio of males to females is not significantly different from 1:1. The reproductive period of the species is protacted (February to June). The peak of the reproductive effort occurs in April–May. The size at first maturity is 68 mm. The population is being heavily exploited.
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  • 48
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    Environmental biology of fishes 59 (2000), S. 29-41 
    ISSN: 1573-5133
    Keywords: sound production ; courtship ; gadidae ; fish ; territorial behaviour
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The codfish family, Gadidae, contains many vocal species. The sounds produced are species-characteristic and relatively simple. Unusually within this family, the haddock Melanogrammus aeglefinus, produces a range of sounds in different contexts. Both male and female haddock produce short sequences of repeated ‘knocks’ during agonistic encounters. During the spawning season, however, male fish produce sounds which vary in their characteristics as courtship proceeds. The repertoire of the male fish consists of a graded series of sounds ranging from a short series of slowly repeated ‘knocks’ to long sounds of rapidly repeated ‘knocks’. The fastest sounds are heard as a continuous humming. Different behavioural acts leading up to the mating embrace are associated with particular sounds, the sounds becoming longer and faster as the level of arousal of the male increases. It is suggested that the sounds serve to bring male and female fish together in the same part of the ocean, and that the sounds also play a role in synchronising the reproductive behaviour of the male and female.
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  • 49
    ISSN: 1573-5133
    Keywords: fish ; Elopiformes ; aquatic respiration ; hypoxia ; gas bladder
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This study quantified the air-breathing frequency (ABf in breaths h−1) and gill ventilation frequency (Vf in ventilations min−1) of tarpon Megalops atlanticusas a function of PO2, temperature, pH, and sulphide concentration. Ten tarpon held at normoxia at 22–33°C without access to atmospheric oxygen survived for eight days, and seven survived for 14 days (at which point the experiment was terminated) suggesting that the species is a facultative, rather than an obligate, air breather. At temperatures of 29°C and below ABf was highest and Vf was lowest at low oxygen partial pressures. Tarpon appear to switch from aquatic respiration to air breathing at PO2levels of roughly 40 torr. The gills were the primary organ for oxygen uptake in normoxia, and the air-breathing organ the primary mechanism for oxygen uptake in hypoxia. At 33°C, both ABf and Vf were elevated but highly variable, regardless of PO2. There were no mortalities in tarpon exposed to total H2S concentrations of 0–232 µM (0–150.9 µM H2S); however, high sulfide concentrations resulted in very high ABf and Vf near zero. Vf was reduced when pH was acidic. We conclude that air breathing provides an effective means of coping with the environmental conditions that characterize the eutrophic ponds and sloughs that juvenile tarpon typically inhabit.
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  • 50
    ISSN: 1573-5133
    Keywords: functional morphology ; habitat heterogeneity ; phenotypic plasticity ; estuary ; durophagy ; intraspecific ; fish
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Archosargus probatocephalusin a Florida estuary was investigated to explore intraspecific variation in prey utilization and jaw biomechanics. Volumetric contribution of major prey types and seven biomechanical features of the oral jaws that characterize prey-capture and processing performance were contrasted between two locations within the estuary. At Mosquito Lagoon, where A. probatocephalusinhabited mostly oyster beds, mangroves and salt marshes, fish consumed mostly thick-shelled bivalves, gastropods, crabs, and tubiculous polychaetes and amphipods. In contrast, conspecifics at Indian River Lagoon that inhabited mostly seagrass beds and algal turf consumed predominantly algae, seagrass, epiphytic invertebrates and small bivalves and gastropods. Difference in magnitude of durophagy between locations was associated with differences in oral-jaw biomechanics. Analyses of covariance indicated that A. probatocephalusat Mosquito Lagoon had more massive jaw muscles and bones, than conspecifics at Indian River Lagoon. Variations in lever ratios for jaw-opening and jaw-closing between locations were not significant. It is hypothesized that intralocality differences in food habits have induced the development of feeding morphologies that enhance the ability of A. probatocephalusto successfully exploit locally dominant prey resources within the estuary. Plasticity of the feeding mechanism of A. probatocephalusmay buffer the species from the adverse effects of settling on heterogeneous habitats that contain variable prey resources such as those found within estuaries.
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  • 51
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    Biogeochemistry 48 (2000), S. 21-51 
    ISSN: 1573-515X
    Keywords: carbon cycle ; decomposition ; global change ; soil organic matter ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences
    Notes: Abstract The world's soils contain about 1500 Gt of organic carbon to a depth of 1m and a further 900 Gt from 1--2m. A change of total soil organic carbon by just 10% would thus be equivalent to all the anthropogenic CO2 emitted over 30 years. Warming is likely to increase both the rate of decomposition and net primary production (NPP), with a fraction of NPP forming new organic carbon. Evidence from various sources can be used to assess whether NPP or the rate of decomposition has the greater temperature sensitivity, and, hence, whether warming is likely to lead to an increase or decrease in soil organic carbon. Evidence is reviewed from laboratory-based incubations, field measurements of organic carbon storage, carbon isotope ratios and soil respiration with either naturally varying temperatures or after experimentally increasing soil temperatures. Estimates of terrestrial carbon stored at the Last Glacial Maximum are also reviewed. The review concludes that the temperature dependence of organic matter decomposition can be best described as: d(T) = exp[3.36 (T − 40)/(T + 31.79)] where d(T) is the normalised decomposition rate at temperature T (in °C). In this equation, decomposition rate is normalised to ‘1’ at 40 °C. The review concludes by simulating the likely changes in soil organic carbon with warming. In summary, it appears likely that warming will have the effect of reducing soil organic carbon by stimulating decomposition rates more than NPP. However, increasing CO2 is likely to simultaneously have the effect of increasing soil organic carbon through increases in NPP. Any changes are also likely to be very slow. The net effect of changes in soil organic carbon on atmospheric CO2 loading over the next decades to centuries is, therefore, likely to be small.
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  • 52
    ISSN: 1573-5168
    Keywords: ELISA ; fish ; immunoassay ; steroids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A simple and rapid Enzyme Linked ImmunoSorbant Assay (ELISA) is described and validated for testosterone, estradiol, and 17,20β-dihydroxy-4-pregnen-3-one (17,20βP). A general procedure for preparation of the acetylcholinesterase labeled steroid is described which is applicable to any steroid. Use of acetylcholinesterase tracer increased the sensitivity of assay so that reliable measurements of each steroid could be achieved with only 10 μl of plasma. The ELISA was applied to measurement of all three steroids every hour for over 24 hours in a female trout using cannulation of the dorsal aorta. This high sampling frequency revealed several short-term (〈2 h) episodic pulses of testosterone and estradiol.
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  • 53
    ISSN: 1573-5028
    Keywords: cell cycle ; kinematic analysis ; leaf development ; light ; temperature ; water deficit
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In planta quantitative studies of cell cycle are necessary for examining the role of cell division in the response of plants to environmental conditions and to analyse the behaviour of transformed plants in this context. We present and discuss non-intrusive kinematic methods which allow estimating the duration of cell cycle with a high spatial resolution in the leaf. Different methods are proposed and discussed for monocotyledons and dicotyledons, and compared with methods involving the use of chemicals. In monocotyledon leaves, cell division is restricted to a limited zone near the leaf insertion point, twice as long in the mesophyll as in the epidermis. In dicotyledons, cell division occurs in the whole leaf with a uniform and constant cell cycle duration for a determinate number of cell cycles, representing about half of leaf development. Over several experiments, this number is well conserved in a given leaf zone in the absence of stresses, but larger near the leaf base than near the leaf tip. After that, cell cycle duration increases because cells are progressively blocked in G1 while the durations of S-G2-M phases do not change with time. Leaf temperature affects neither the distribution of nuclei in each phase of the cycle nor the number of cell cycles in a leaf. Water or light deficits both cause a partial blockage of nuclei in G1 during the stress only, thereby increasing cell cycle duration and decreasing final cell number. These results suggest that a strong developmental programme drives cell division in leaves, so a simple framework allows analysis of temporal patterns, of spatial gradients and of the effect of environmental conditions.
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  • 54
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    Fish physiology and biochemistry 22 (2000), S. 61-66 
    ISSN: 1573-5168
    Keywords: blastomere ; embryo ; fish ; salmonid ; transcriptional inhibitor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Early embryonic development is dependent on proteins translated from messenger ribonucleic acid (mRNA) molecules of maternal origin and the embryonic genome. The present study examined the importance of maternal mRNA during early embryogenesis in the rainbow trout (Oncorhynchus mykiss), and developmentally, when the embryonic genome becomes transcriptionally active. To address the significance of maternal mRNA, eggs were exposed to the translational inhibitor cycloheximide (CHX) for 12 h post-fertilization. Concentrations of CHX beginning at 1 μM significantly delayed the initial stages of cleavage. Exposed embryos had only reached the 4-cell stage, while a significant proportion of the eggs treated with 0.1 μM CHX or no CHX (i.e., control) had attained the 8-cell stage. Treatment of fertilized eggs with the transcriptional inhibitor actinomycin D (AMD) was utilized to demonstrate when synthesis of embryonic mRNA begins. Rainbow trout eggs exposed to 16 μM AMD were unaffected after 12 h, but by 9 days post-fertilization had not developed the embryonic keel characteristic of the controls. To determine when transcriptional inhibition could first be demonstrated AMD exposed embryos were dissected free of the egg, between days 2 and 6 of incubation, and individual blastomeres prepared for diameter measurements. After 3 days of exposure to 16 μM AMD embryonic blastomeres were significantly larger than the control group (P〈0.05), which normally decrease significantly in size due to repeated cellular division. This showed that AMD interfered with nuclear transcription preventing the progression of cellular events necessary for normal cleavage, and indicates that transcriptional activation of the embryonic genome in rainbow trout occurs about 3 days post-fertilization.
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  • 55
    ISSN: 1573-5168
    Keywords: amino acid sequence ; fish ; glutathione ; glutathiolation ; GST ; mass spectrometry ; PCB
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Liver, kidney, gill and olfactory epithelium cytosolic fractions of rainbow trout (Oncorhynchus mykiss) were examined for glutathione S-transferase (GST) contents. Proteins retained on a glutathione (GSH)-affinity matrix were separated as monomers by reversed-phase HPLC and characterized by immunoblotting, mass spectrometry and partial amino acid sequence. For each organ concerned, a specific pattern of these proteins was determined and appeared similar for liver and kidney on one hand, and for gill and olfactory epithelium on the other hand. It was confirmed that the prominent hepatic GST is a class π enzyme, also constitutively expressed as a major isoform in the four organs studied. Moreover, a class π variant and two new class μ GST subunits were characterized in minor fractions. An unknown protein, which was found major in gills and olfactory epithelium, exhibited some characteristics of class θ GSTs. Occurrence of possible GSH-adduct formation observed on two distinct monomers in specific experimental conditions is discussed. These results and methods were used to investigate the effect of 3,3′,4,4′-tetrachlorobiphenyl (TCB), a polychlorinated biphenyl (PCB), on GST expression in trout liver. From HPLC-profiling, significant co-induction of the major class π and the two minor class μ GST subunits was observed in trout after waterborne exposure to TCB which was followed by a slight increase in 1-chloro-2,4-dinitrobenzene (CDNB) activity. The present work allows qualitative evaluation of the specific detoxification potential of rainbow trout. The use of HPLC-profiling of GSTs as a possible tool for the biomonitoring of polluted aquatic environment is suggested.
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  • 56
    ISSN: 1573-5168
    Keywords: development ; endocrine regulation ; environment ; fish ; plasma proteins ; radioimmunoassay ; smoltification ; teleost
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Free plasma thyroxine (FT4) levels were measured in coho salmon, Oncorhynchus kisutch, during parr-smolt transformation (smoltification) using an equilibrium dialysis system followed by a radioimmunoassay. The FT4 data were correlated to total plasma thyroxine (TT4), triiodothyronine (TT3), and growth hormone (GH). Plasma samples were taken weekly from early April to late May, when the salmon where released from the hatchery. Free thyroxine and GH levels increased gradually through smoltification. TT4 levels increased significantly in mid-April and in mid-May. TT3 levels increased in April and remained elevated until late-May after which they declined to the lowest levels. During the first increase in TT4 levels, FT4 levels remained low and TT3 levels did not increase until FT4 levels increased in late-April. In addition, after TT4 levels decreased in late May, FT4 levels remained elevated. These data show that there are differences between the plasma FT4 and TT4 profiles during smoltification. Nevertheless, regression analysis indicates that FT4 levels are highly correlated to the increases in the levels of GH (r=0.73) and TT4 (r=0.70). In addition, GH is less correlated to TT4 and TT3 (r=0.24 and r=−0.46, respectively) compared with FT4 (r=0.73), suggesting a close relationship between the increases of FT4 and GH. In addition, these data suggest that this method of measuring free plasma thyroxine may provide a new tool for studying the timing of thyroid hormone action and regulation during parr-smolt transformation in salmonids.
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  • 57
    ISSN: 1573-5168
    Keywords: air ; ATP ; egg ; fertilization ; fish ; hyperoxia ; hypoxia ; oxygen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract To further examine the concept of egg quality and the physiology of stored salmonid eggs, we investigated the effects of different oxygen tensions on the adenosine triphosphate (ATP) levels of unfertilized, activated, and fertilized chinook salmon (Oncorhynchus tshawytscha) eggs. The ATP levels of unfertilized chinook salmon eggs were 2.61±0.14 nmol ATP per egg (17.6±0.9 μmol l−1 relative to cell water) and ranged from 1.98 to 3.63 nmol ATP per egg. The ATP content of unfertilized eggs maintained at 10 °C under 100% O2, 21% O2, and 100% N2 remained unaltered throughout a 120 h storage period. Storing eggs under identical conditions at 20 °C (in an effort to speed egg metabolism and ATP turnover) resulted in significant O2-independent decreases in ATP levels. However, ATP levels of unfertilized eggs exposed to 1 mmol l−1 potassium cyanide (a potent inhibitor of oxidative phosphorylation) at 10 °C were significantly decreased after 24 h and continued to decline throughout the 120 h maintenance period to about 30% of time=0 values. Maintenance with exogenous nutrients (5 mmol l−1 acetate plus 5 mmol l−1 pyruvate) over 120 h at 10 °C did not alter the ATP content of unfertilized eggs. Eggs activated by exposing them to 10 °C water for a few minutes showed a rapid decrease in ATP values, regardless of whether the eggs were fertilized or not. Following an initial ∼25% drop after fertilization, the ATP levels remained stable for the remainder (5 d) of the incubation period in eggs maintained in 10 °C water. Therefore, unfertilized chinook salmon egg ATP levels appear to be relatively stable and maintained by a low, cyanide-inhibitable metabolism. The stability of egg ATP levels may be one reason that salmonid eggs can be stored for several days while eggs from other fishes cannot.
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  • 58
    ISSN: 1573-5168
    Keywords: epithelial damage ; fish ; intestine ; lipid digestibility ; lipid droplets ; lipid nutrition ; lipoprotein synthesis ; ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Arctic char (Salvelinus alpinus L.) were fed over a three-week period with a commercial diet or one of seven casein-based diets. The latter were either lipid-free or supplemented with 50–200 g linseed oil kg−1 diet by dry weight, 160 g linseed oil and 40 g 14:0 or 160 g linseed oil and 40 g 16:0. Three fish having filled guts were sampled from each dietary group and analysed for ultrastructural changes in the pyloric caecum. Increasing the dietary lipid level increased the accumulation of lipid droplets in columnar absorptive enterocytes from about 9% of epithelial volume in fish fed a diet of 50 g linseed oil, to almost 61% in fish fed a diet of 200 g linseed oil. Replacing linseed oil in the diet with 14:0 (160 g linseed oil + 40 g 14:0 kg−1 diet) appeared to produce a smaller lipid loading (roughly 53%) but the difference was not statistically significant. However, replacing 40 g linseed oil with 40 g of 16:0 in the diet decreased lipid loading significantly to just under 10%. Epithelial damage to enterocytes was assessed using a ranking scale based on ultrastructural signs of cell and organelle swelling and degeneration. The extent of damage closely followed the level of lipid loading, being lowest in fish fed the lipid-free or low-lipid (damage index 0.07–0.13) diets, and highest in char maintained on a diet containing 200 g linseed oil (index 1.41). Replacing linseed oil with 14:0 (160 g linseed oil + 40 g 14:0) appeared to reduce the damage index to 0.77 but this was not significant. However, a significant reduction of the damage index to 0.27 was observed when linseed oil was replaced by 16:0. We conclude that higher dietary linseed oil promotes lipid droplet accumulation in enterocytes. The droplets are probably related to the amount of polyunsaturated fatty acids in the lipid. Intracellular droplet formation and cellular damage are both reduced by adding saturated fatty acids to the diet. This could be related to disruptions in the lipoprotein assembly rate. The cellular damages observed with high lipid diets are likely to be pathological and may lead to intestinal malfunction and represent a major infection route for pathogenic bacteria.
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  • 59
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    Fish physiology and biochemistry 22 (2000), S. 303-310 
    ISSN: 1573-5168
    Keywords: ACTH ; CRH ; fish ; leukocyte cell lines ; peripheral leukocytes ; neuroendocrine-immune function ; POMC
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The interrelationships between the neuroendocrine and immune systems are becoming more understood, at least in mammalian systems. The most characterized of these relationships is that of hormonal signaling within the hypothalamo-pituitary-adrenal (HPA) axis. CNS-perceived signals stimulate the release of corticotropin releasing hormone (CRH) which in turn stimulates the release of pituitary corticotropin (ACTH) and ultimately the release of adrenal-cortex-derived corticosteroids. We demonstrate that channel catfish peripheral blood mononuclear cells, a channel catfish B-cell line (1G8) and a T-cell line (28S.1), constitutively and in response to CRF, secrete a molecule that is reactive with a mammalian RIA for ACTH (irACTH). The T-cell line was the most responsive to CRH and may provide a valuable model for understanding the interrelationships between the neuroendocrine and immune systems in lower vertebrates. Lymphoid derived ACTH, or ACTH-like products, in fish, as well as higher vertebrates, may represent a paracrine or autocrine control on lymphocyte function and immune regulation.
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  • 60
    ISSN: 1573-5168
    Keywords: chloride cells ; Epinephelus coioides ; grouper ; Na+,K+-ATPase activity ; salinity ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The activity of the enzyme Na+,K+-ATPase and morphological changes of gill chloride cells in grouper, Epinephelus coioides larvae and juveniles were determined 6–48 h after abrupt transfer from ambient rearing conditions (30–32 ppt, 26.5–30 °C) to different salinity (8, 18, 32, 40 ppt) and temperature (25, 30 °C) combinations. Na+,K+-ATPase activity in day 20 larvae did not change at salinities 8–32 ppt. Activity decreased significantly (P 〈0.01) after exposure to 40 ppt at 25–30 °C, which was accompanied by an increase (P 〈0.05) in density and fractional area of chloride cells. Enzyme activity in 40 ppt did not reach a stable level and larvae failed to recover from an osmotic imbalance that produced a low survival at 25 °C and death of all larvae at 30 °C. Enzyme activity and chloride cell morphology in day 40 groupers did not change in 8–40 ppt at 25 °C and 8–32 ppt at 30 °C. A significant decrease and a subsequent increase in Na+,K+-ATPase activity in 40 ppt at 30 °C was associated with the increase in chloride cell density resulting in an increased fractional area but a decreased cell size. Enzyme activity and chloride cells of day 60 grouper were unaffected by abrupt transfer to test salinities and temperatures. These results demonstrate that grouper larvae and juveniles are efficient osmoregulators over a wide range of salinities. Salinity adaptation showed an ontogenetic shift as the larvae grew and reached the juvenile stage. This development of tolerance limits may reflect their response to actual conditions existing in the natural environment.
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  • 61
    ISSN: 1573-5168
    Keywords: brush border hydrolases ; dietary lipids ; fatty acids ; fish ; intestine ; membrane lipid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Triplicate groups of juvenile seabass (initial weight of 241 g) were fed during 13 weeks three isonitrogenous experimental diets containing different lipid levels, 12% (LL group), 21% (ML group) and 30% (HL group). At the end of the experiment, fish weight gain was similar among the 3 dietary groups. Intestinal brush border membranes were purified for each dietary group; one part of the brush border fraction was dedicated to enzyme assays, the remaining fraction being used for lipid extraction followed by fatty acid analysis. The fatty acid composition of the brush border membrane differed among the 3 groups, although the 3 experimental diets had the same fatty acid composition. The increase in dietary lipid level resulted in a lowering in (n-3) polyunsaturated fatty acid (PUFA) paralleled with an increase in monounsaturated fatty acid. A significant reduction in the brush border enzyme activities, namely alkaline phosphatase, aminopeptidase N, γ-glutamyl transpeptidase and maltase, was also observed with the elevation of the dietary lipid level. The change in activity of intestinal digestive enzymes, which are membrane-bound proteins, could be attributed to the modification of fatty acid composition and fluidity of the brush border membranes (BBM). Such lowering in PUFA and increase in monounsaturated fatty acid in BBM, concomitant with a decline in membrane enzymatic activity, has been described as a malnutrition indicator in mammals. It raises the question of possible disorders of gut functions in fish fed increasing lipid levels.
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  • 62
    ISSN: 1573-5168
    Keywords: Atlantic cod ; temperature ; melatonin ; photoperiod
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This study investigated the effects of photoperiod and temperature on plasma melatonin secretion in Atlantic cod (Gadus morhua L.). Initial work confirmed the presence of a diel profile of melatonin synthesis, with elevated levels during the dark phase. Unusually for fish, the peak in plasma melatonin occurred towards the end of the dark phase, which is indicative of a type `A' melatonin profile. When exposed to 60 hours of continuous darkness a clear endogenous rhythm of melatonin synthesis was observed, which continued for 4 cycles with a periodicity which, approximated to 24 h. When acclimated to varying temperatures (4, 8, 12 or 16 °C) no variation in melatonin production was seen, however, body size appeared to be an important influence, with the smallest fish exhibiting significantly higher levels of dark phase melatonin. Finally, the application of additional night-time illumination to cod maintained in sea cages i.e. without blackout, did not significantly reduce dark phase plasma melatonin, suggesting that cod are less sensitive to photoperiod manipulation in cages than salmonids.
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  • 63
    ISSN: 1573-5168
    Keywords: cortisol ; ACTH ; α-MSH ; GH ; head kidney ; pituitary ; temperature ; stress ; Sparus aurata
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This study investigated the effects of a drop in water temperature (18 °C to 9 °C in 24 h) on the pituitary and interrenal hormones of the gilthead sea bream Sparus aurata. The in vitro sensitivity of the interrenal tissue to ACTH, plasma levels of cortisol, ACTH, α-MSH, GH, glucose, lactate and ions were determined. In vitro ACTH, stimulated the release of cortisol from isolated interrenal glands from control gilthead sea bream in a concentration dependent fashion. However, the interrenal cells were less sensitive to ACTH as soon as 24 h following the onset of the temperature drop. At this time, plasma cortisol and ACTH levels were raised, and plasma GH concentrations were decreased, whereas no significant changes were found in plasma α-MSH. After 96 h plasma ACTH levels had recovered whereas plasma cortisol levels were still higher than controls after 8 days of the beginning of the experiment. Interrenal sensitivity had recovered after 8 days. The results may help to clarify the relationship between the stress response and the aetiology of the winter syndrome in sea bream.
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  • 64
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    Fish physiology and biochemistry 23 (2000), S. 327-336 
    ISSN: 1573-5168
    Keywords: adrenaline ; cortisol ; fish ; heat shock proteins ; stress proteins ; stress response
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The relationship between stress protein (SP) levels and the hormonal stress response in salmonids was examined through the measurement of gill SP70 and SP30 levels together with plasma cortisol, glucose and ion concentrations, in response to handling stress (45 s holding in a net), intraperitoneal cortisol implants, and heat shock (+10 °C). Handling and cortisol implants resulted in increased plasma cortisol and glucose levels. Heat shock following handling reduced plasma [Na+] below that observed in response to the handling stress alone, and heat shock following cortisol implant significantly lowered both plasma [Cl−] and [Na+] below that of fish receiving the cortisol implant alone. Increased SP70 levels occurred 1 h following the 2 h +10 °C heat shock. Handling the fish prior to the application of heat shock suppressed the increase of SP70 levels in the gills. However, increased plasma cortisol concentrations alone did not attenuate gill tissue SP70 increase caused by heat shock. Physiological (10−7 M) and pharmacological (10−5 M) concentrations of adrenaline caused increased levels of SP70 in hepatocytes. Addition of the β-blocker propanolol blocked this response to adrenaline. The results indicate that handling procedures do not result in an increase of hsp30 or hsp70 and may suppress hsp synthesis under certain circumstances.
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  • 65
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    Reviews in fish biology and fisheries 10 (2000), S. 293-323 
    ISSN: 1573-5184
    Keywords: anthropogenic ; cytonuclear ; disequilibrium ; fish ; Gambusia ; hybridization ; mtDNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Interspecific hybridization occurs widelyacross a taxonomically diverse array of fishspecies. Multiple factors typically interactto effect the outcome of hybridization events. Human influences have been frequently cited ascontributing factors (nearly 50% of reviewedcase studies). Aquacultural activities,species introductions, and loss or alterationof habitats were frequently implicated. Wehighlight the utility of genetic markers andnovel methods of statistical analysis forinferring the extent, rate, direction, andlikely causes of hybridization. Emphasis isplaced on cytonuclear genetic systems. Wedemonstrate the utility of cytonuclear modelsfor hypothesis testing using empirical data.
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  • 66
    ISSN: 1573-6857
    Keywords: altitude ; clines ; latitude ; phenotypic variability ; temperature ; Zaprionus indianus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We analyzed natural populations of Zaprionus indianusin 10 Indian localities along a south-north transect (latitude: 10–31°3 N). Size traits (body weight, wing length and thorax length) as well as a reproductive trait (ovariole number) followed a pattern of clinal variation, that is, trait value increased with latitude. Wing/thorax ratio, which is inversely related to wing loading, also had a positive, but non-significant correlation with latitude. By contrast, bristle numbers (sternopleural and abdominal) exhibited a non-significant but negative correlation with latitude. Sex dimorphism, estimated as the female/male ratio, was very low in Z. indianus, contrasting with results already published in other species. Genetic variations among populations were also analyzed according to other geographic parameters (altitude and longitude) and to climatic conditions from each locality. A significant effect of altitude was found for size traits. For abdominal bristles, a multiple regression technique evidenced a significant effect of both latitude and altitude, but in opposite directions. Genetic variations were also correlated to climate, and mainly with average year temperature. Taking seasonal variations into account failed however to improve the predictability of morphometrical variations. The geographic differentiation of Z.indianusfor quantitative traits suggests adaptive response to local conditions, especially to temperature, but also reveals a complex situation according to traits investigated and to environmental parameters, which does not match results on other drosophilid species.
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  • 67
    ISSN: 1573-6857
    Keywords: Astyanax scabripinnis ; B chromosome ; Characidae ; cytogenetic ; fish
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Specimens of Astyanax scabripinnisfrom three different altitudes (1920, 1800 and 700 m) along the Ribeirão Grande stream in the Campos do Jordão region (São Paulo State, Brazil) were investigated. The same diploid number, 2n = 50, was detected in the three populations, with the following karyotypic constitution: 6M, 22SM, 10ST and 12A. The populations located at 1920 and 1800 m altitude presented a high incidence of B chromosomes varying in number (0–2), shape (meta- and submetacentrics), size (large and small) and sex-related frequency (they were more frequent among females). The two morphologically variant B chromosomes probably evolved from a metacentric macrochromosome, which is the most commonly observed B chromosome in several A. scabripinnispopulations.
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  • 68
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    World journal of microbiology and biotechnology 16 (2000), S. 297-301 
    ISSN: 1573-0972
    Keywords: Anaerobic bacteria ; growth ; protease ; psychrotrophs ; temperature ; volatile fatty acids
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Five anaerobic proteolytic bacteria were isolated from water bodies of Leh, India, where the ambient temperature varies from −25 to 25 °C. Isolates showed growth at all temperatures ranging from 5 to 37 °C except SPL-4 and SPL-5 which showed no growth at 5 °C. The cultures could grow and produce proteases on various protein substrates and the yield varied with the substrates. Two of the cultures showed the presence of spores. Acetate was the dominant VFA during hydrolysis of protein substrates.
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  • 69
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    World journal of microbiology and biotechnology 16 (2000), S. 571-572 
    ISSN: 1573-0972
    Keywords: Anaerobes ; hydrogen sulphide ; rubber stoppers ; sulphate reduction ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Common black rubber stoppers, made from natural rubber and styrene–butadiene, may cause a loss of hydrogen sulphide from aqueous media and impede the growth of sulphate-reducing bacteria under thermophilic conditions.
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  • 70
    ISSN: 1573-0972
    Keywords: Carbondioxide ; fungi ; oxygen ; Rhizopus ; solid-substrate fermentation SSF ; tempe modelling ; temperature ; water activity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Rhizopus microsporus var. microsporus and var. oligosporus are used in the manufacture of various Asian fermented foods (tempe, black oncom, sufu). In view of solid-substrate fermentation (SSF) control, mycelial growth of strains of both varieties was tested for sensitivity to fluctuations of temperature, water activity and interstitial gas composition. This was achieved by measuring radial growth as well as biomass dry weight of pre-germinated microcolonies on defined media. The optimum conditions were temperature 40 °C, a w 0.995 and a gas composition of air for the growth of both strains on a model medium. Whereas radial growth rates of var. microsporus and var. oligosporus were similar, biomass growth rates of var. oligosporus were higher than those of var. microsporus under optimum conditions. The temperature-dependent growth of Rhizopus spp. at a w 〉 0.98 could be described by the Ratkowsky Equation. Carbon dioxide (5–10% v/v) inhibited the growth of Rhizopus spp. at non-limiting levels of oxygen. The two strains were able to grow at low (0.5% v/v) oxygen levels, but the mycelial density was rather low. No interrelation of water activity and gas composition was observed, but at high water activity the fungi were more sensitive to changes of temperature. The implications for process control are discussed.
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  • 71
    ISSN: 1573-0662
    Keywords: monoterpene emission ; Mediterranean pine ; seasonal variation ; light ; temperature ; algorithms ; model
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Geosciences
    Notes: Abstract Current inventories of terpenes released from vegetation consider only the short-term influences of light and temperature on emissions to simulate temporal variation during the year. We studied whole canopy emissions from young Pinus pinea during a 15-month enclosure in greenhouse chambers and examined data for other long-term influences. Mean daytime emission rates strongly increased during spring, reached an annual maximum of ≈ 200 pmol m−2 total needle area s−1 (1.1 μg g−1 leaf dry weight h−1) between mid June and mid August, strongly declined in fall and reached an annual minimum of ≈ 1 pmol m−2 s−1 (0.006 μg g−1 h−1) between January and February. Normalization to standard temperature and light conditions did not change the annual time course of emissions, but reduced summer to winter ratio from a factor of 200 to about 45. Seasonal variation was characterized also by changes in terpene composition: among the six main compounds, three (t-β-ocimene, linalool, 1.8-cineol) were exclusively emitted during sunlit hours in the main vegetation period, whereas the other (limonene, α-pinene, myrcene) were emitted day and night and throughout the seasons. The results suggest that different terpene sources in P. pinea foliage exist and that a great part of the annual emission course observed here results from seasonal influences on these sources. A global model to simulate plant emissions is proposed, which accounts for seasonal influences on emissions in addition to the short-term effects of temperature and light. The model is tested on field data and discussed for its general application.
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  • 72
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    Experimental and applied acarology 24 (2000), S. 579-596 
    ISSN: 1572-9702
    Keywords: Tetranychus urticae ; ambulatory dispersal ; temperature ; humidity ; mortality ; emigration ; immigration ; biological control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In a greenhouse and in an open field, aspects of aerial and ambulatory dispersal of the phytoseiid mite, Neoseiulus fallacis (Garman) were studied with a focus on events that would occur after aerially dispersing mites had landed on soil or associated substrates. We measured recovery of predators on lima bean plants (Phaseolus lunatus L.) that were infested with the two-spotted spider mite, Tetranychus urticae Koch. Factors thought to affect movement and colonization were distance to a receiver unit from a release (landing) point, intervening soil surfaces such as clods, gravel, fine soil and grass, and management of soil surfaces such as mulching, watering or both. In the field, the effect of distance (0.11–1.76 m) from a landing point to a receiver unit was significant, with a negative log-linear relationship. Soil surfaces such as clods and management actions such as watering with mulching allowed for more capture of predators on bean plants with prey than did other treatments. Environmental conditions greatly affected survival of N. fallacis.Predators in the field that were present on bare soil suffered high mortality (ca. 90%) at fluctuating daytime conditions of 26.4 ± 4.8°C and 56 ± 13.4% RH. Predators only suffered 10% mortality in the greenhouse under the same setting, but under more controlled and favorable environmental conditions. Effects of environmental conditions, mode of dispersal and implications to biological control are discussed.
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  • 73
    ISSN: 1573-5095
    Keywords: Coastal Plain sites ; Pinus echinata Mill. ; P. taeda L. ; precipitation ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Seed production was monitored during24 years using seed-collection traps inloblolly–shortleaf pine (Pinus taeda L.–P.echinata Mill.) stands located in southeast Arkansas,north-central Louisiana, and southwest Mississippi onthe southeastern Coastal Plain, USA. Sound seedproduction was correlated with mean monthlyprecipitation and temperature from National Oceanicand Atmospheric Administration weather stationslocated near the seed-collection areas to determinethe potential of weather factors in forecasting pineseed crops. Correlations were restricted to threecritical periods in the pine reproductive cycle –strobili primordia differentiation, pollination, andfertilization. The most important (P ≤ 0.05)variables correlated with pine seed production for combined locations were cumulative precipitation (r = +0.60) during July, August, and September at 27 to 25 months before seed dispersal and mean temperature (r = −0.45) in August at 26 months before seed dispersal. Because multiple environmental factors can negatively impact pine seed development during the two yearsfollowing strobili primordia differentiation,seed-production forecasts based on weather variablesshould be verified by on-site cone counts during thesummer preceding autumn seed dispersal.
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  • 74
    ISSN: 1573-5036
    Keywords: Acacia ; Libya ; root-nodulating bacteria ; salinity ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Thirty isolates of root-nodulating bacteria obtained from Acacia cyanophylla, A. karroo, A. cyclops, A. tortilis (subsp.raddiana), Faidherbia albida and Acacia sp., grown in different regions of Libya, were studied by performing numerical analysis of 104 characteristics. Three fast- and one slow-growing reference strains from herbaceous and woody legumes were included. Five distinct clusters were formed. The fast-growing reference strains were separated from the isolates whereas the slow-growing was included in cluster 4. With the exception of one cluster, the majority of clusters were formed regardless of the host plant or site of origin. Based on plant tests, generation times, acid production and carbon utilization the isolates were diverse (fast and slow-growing isolates). Like slow-growing isolates, most of the fast-growing isolates appeared to be non-specific, nodulated many species from the same genus notably F. albida, known to nodulate only with slow-growing strains. Most clusters grew at temperatures 35 °C and 37 °C; some grew at temperatures above 40 °C. The majority of isolates grew at acid and alkaline pH and only one isolate grew below pH 4. Most isolates were able to utilize many amino acids as nitrogen sources and to reduce nitrate. Urea was hydrolysed by all clusters. Monosaccharides and polyols were used by slow and fast-growing isolates as the only carbon sources whereas assimilation of disaccharides varied: Some isolates, like slow-growing isolates, failed to utilize these carbon sources. Most isolates were unable to utilize polysaccharides. Regarding tolerance to NaCl on agar medium, the majority of isolates were unable to grow at a concentration of 2% NaCl, but some were highly resistant and there was one isolate which grew at 8% NaCl. Most isolates were resistant to heavy metals and to antibiotics.
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  • 75
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    Plant cell, tissue and organ culture 61 (2000), S. 59-67 
    ISSN: 1573-5044
    Keywords: alar ; in vitro plantlet ; mannitol ; nitrogen ; rooting ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The importance of leaf area of in vitro propagated potato (Solanum tuberosum L.) plantlets for further growth during acclimatisation and the after-effects of in vitro treatments on growth were examined. The in vitro treatments included different levels of alar, nitrogen or mannitol or different temperatures during the last in vitro phase, the rooting phase. Leaf area or ground cover was recorded one day after planting to soil and at the end of the first phase of ex vitro growth, the acclimatisation phase. Regression analysis showed that leaf area of a transplant at the end of acclimatisation phase was positively influenced by leaf area of the same plantlet at the beginning of the phase. The relative increase in leaf area during acclimatisation (increase/early leaf area) was linearly related to the inverse of the early leaf area, indicating almost comparable relative increases for plantlets having larger early leaf areas, but more variable responses for plantlets having smaller early leaf areas. In vitro treatments mainly affected leaf area of transplants through their effects on early leaf area. Adding alar, reducing nitrogen and reducing temperature increased leaf area. Reducing mannitol increased ground cover. A lower nitrogen concentration and higher temperature in some cultivars had slight negative effects on the relative increase in leaf area after acclimatisation. For nitrogen these negative effects were less significant than the positive effects through early leaf area. Results stress the importance of manipulation of leaf area in vitro to enhance plant performance in later stages of growth.
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  • 76
    ISSN: 1573-5117
    Keywords: urban runoff ; in situ bioassay ; Gammarus minus ; heavy metals ; water quality ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Thompson Run, a headwater stream in central Pennsylvania (U.S.A.), supports an impaired macroinvertebrate community downstream of the outlet of a detention pond that receives urban runoff. To determine if toxicity from the metals or other pollutants in urban runoff contributed to impairment, we exposed adult, male Gammarus minus to urban runoff during a 42-day in situ bioassay that included 12 rain events. Test animals were collected from a site upstream of the detention pond outlet using two methods: precopula pair separation and sieving. Water quality, temperature and tissue metal concentrations were measured during the bioassay. The survival of precopula G. minus was lower (p=0.048) at a site downstream of the detention pond outlet compared to a site upstream of it, but the survival of sieved G. minus was not different between sites (p=0.803). Large hourly increases in temperature (up to 6.6 °C) and major reductions in water quality including order of magnitude increases in suspended materials (measured as turbidity) and the concentrations of copper, zinc and lead occurred downstream of the detention pond outlet during stormflow (i.e. following rain events). In contrast, changes in temperature and water quality were minor upstream of the pond outlet throughout the bioassay. Copper and cadmium concentrations in leaf samples and copper, zinc and lead concentrations in G. minus samples were significantly higher downstream of the pond outlet than they were upstream of it. Despite harsh conditions downstream of the pond outlet (i.e. metal contamination, inputs of suspended materials and rapid temperature increases), the in situ bioassay did not convincingly demonstrate that urban runoff was toxic to adult, male G. minus.
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  • 77
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    Hydrobiologia 422-423 (2000), S. 257-270 
    ISSN: 1573-5117
    Keywords: fish ; biotest ; Index of Biotic Integrity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The species composition, community structure, trophic composition, abundance and biomass were investigated in 60 Lithuanian rivers. Leaning on the results of investigations, four main types of river fish communities (fish communities of the brooks, streams, middle-sized and large rivers) differing in species number and composition, trophic composition, density and biomass were singled out. Referring to the characteristic features of fish communities, the Index of Biotic Integrity (Karr et al., 1986) was modified and adapted to the conditions of Lithuania. In order to test biointegrity, nine metrics in three categories (species composition, abundance and biomass; trophic composition and fish condition) were selected. The metrics of this biotest assess attributes that are assumed to be correlated with biotic integrity. These metrics, by the sum of their ratings, characterize the underlying biotic integrity of the sampling site. Six integrity classes (excellent, good, fair, poor, very poor and no fish) of river fish communities were distinguished. Individual tests were adapted to brooks (up to 10 km in length), streams (up to 50 km and riverhead of bigger rivers), middle-sized rivers (up to 200 km and the middle reaches of the large rivers) and large rivers (over 200 km and the lower reaches of the middle-sized rivers). While brooks and streams mainly show intact conditions (class 1 and 2), middle-sized and large rivers are significantrly more impacted (class 2 to 3).
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  • 78
    ISSN: 1573-5141
    Keywords: Canada ; dissolved oxygen ; fish ; ice cover ; macroinvertebrates ; pulp mill effluent ; rivers ; sewage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract To determine whether existing dissolved oxygen (DO) regulationswere adequate to protect riverine biota, the Northern River Basins Studyrequired a research and assessment program to establish the effect ofpulp mill and municipal sewage discharges on under-ice DO concentrationsand aquatic biota in the Athabasca, Wapiti and Smoky rivers of northernAlberta, Canada. Analysis of monitoring data collected over 〉30 yearsshowed that pulp mill and municipal effluents have caused “sags” in DObelow their discharges and contributed to an increased rate of declinein DO over 10s of kilometers in the Wapiti-Smoky rivers or 100s ofkilometers in the Athabasca River. To permit forecasting of DO declinesin response to changes in river discharge or effluent biochemical oxygendemand (BOD), a one-dimensional steady-state river water quality modelwas implemented that successfully (r2 =0.74–0.92) predicted DO concentrations over an 820 km reach of theAthabasca River for the 1990–1994 winters. Experiments on nativefish and benthic macroinvertebrate species showed that exposure to lowDO and low temperatures (2–3°C) caused delays in hatch ofmountain whitefish eggs (at 6.5 mg/L DO), reduced mass of bull troutalevins post-hatch (at 5.0 mg/L DO), extended the spawning period ofburbot (at 6.0 mg/L DO), and depressed feeding rates and loweredsurvival of the mayfly Baetis tricaudatus (at 5 mg/L DO).As a result of recommendations from this integrated program of rivermonitoring, modelling and experimentation, effluent BOD loading has beenreduced to the Athabasca and Wapiti rivers and the provincial DOguideline for protection of aquatic life has been increased from 5.0 to6.5 mg/L.
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  • 79
    ISSN: 1573-6849
    Keywords: fish ; fluorescent in-situ hybridization ; meiosis ; repetitive DNA ; synaptonemal complexes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A method of preparing two-dimensional surface spreads of fish synaptonemal complexes (SCs) associated with fluorescent in-situ hybridization is described. This technique permits a novel approach to the analysis of chromatin organization and the construction of physical maps at meiosis, since surface-spread pachytene chromosomes are several times the length of metaphase chromosomes and the decondensed chromatin loops are attached to the lateral elements of the SC. We have applied this technique to analyze the location and organization of three different repetitive DNA sequences, rDNA, an EcoRI satellite DNA of the Sparidae family and telomere DNA in the gilthead seabream Sparus aurata. Our observations indicate that, depending on the type of sequence, the chromatin has different properties with regard to anchorage to the SC.
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  • 80
    ISSN: 1573-6849
    Keywords: chromosomes ; fish ; highly repetitive DNA ; 5S rDNA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
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  • 81
    ISSN: 1573-6857
    Keywords: Apareiodon affinis ; cytogenetics ; fish ; polymorphism ; sex chromosomes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Comparative cytogenetic studies were carried out on Apareiodon affinis from an important hydrographic system at South America, the Paraná river basin. Two distant regions were chosen, which were separated by Guaíra Falls (formerly Sete Quedas); the region in the upper part of the hydrographic basin is called Upper Paraná (Brazil), whereas and the other in the lower part is called Lower Paraná (Argentina). Individuals from Upper Paraná have diploid numbers of 2n = 54 (NF = 108) for males and 2n = 55 (NF = 110) for females, showing female heterogamety with a ZZ/ZW1W2 multiple sex chromosomes system that is endemic for the region. In different localities at Lower Paraná, the specimens presented diploid number of 2n = 54 for both sexes, without any sex chromosomes heteromorphism. However, they have an accentuated polymorphism characterized by variation in number of acrocentric chromosomes, constituting something new for family Parodontidae. The most likely hypothesis to explain the origin of such polymorphism is based on successive pericentric inversions giving rise to acrocentric chromosomes. Thus, it was possible to detect 10 cytotypes along the Lower Paraná basin. Such chromosomal variations possibly are the consequence of an adaptative process. Our data probably indicate the occurrence of distinct species in each region that share the same denomination.
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  • 82
    ISSN: 1573-5117
    Keywords: immunostimulation ; plant extracts ; fish ; antibody response ; neutrophil activity ; disease resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Effect of leaf extract of Ocimum sanctum on (i) the specific and non-specific immune responses and (ii) disease resistance against Aeromonas hydrophila was investigated in Oreochromis mossambicus. Sheep red blood cells (SRBC) and Heat aggregated bovine serum albumin (HA-BSA) were used as antigens for specific and non-specific immune response studies, respectively. Antigens were administered through intraperitoneal route. Anti-SRBC antibody titres were determined by direct haemagglutination and anti-bacterial antibodies were determined by bacterial agglutination. Nitroblue tetrazolium (NBT) assay was used to determine neutrophil activity. Disease resistance was measured in terms of relative percent survival (RPS). The immunostimulatory effect of the leaf extract of O. sanctum, when administered through intraperitoneal and oral routes was obvious. Leaf extract of O. sanctum, when administered intraperitoneally, stimulated both antibody response and neutrophil activity. Dietary intake of O. sanctum also enhanced the antibody response and disease resistance against A. hydrophila. Possibility of using O. sanctum as immunostimulant in the maintenance of finfish health in intensive freshwater aquaculture is suggested.
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  • 83
    ISSN: 1573-5117
    Keywords: Belgium ; Flanders ; rivers ; Index of Biotic Integrity ; fish ; water pollution ; ecological water quality
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A multimetric fish Index of Biotic Integrity (IBI) was composed to assess the biotic integrity of Flandrian water bodies. As fish communities differ substantially between standing waters, running waters of the bream zone and running waters of the barbel zone, eight candidate metrics for each of these water types or zones were identified, representing three major classes of biological attributes. These are species richness and composition, fish condition and abundance, trophic composition. The metrics were tested and modified where needed. The IBI was applied throughout Flanders on 104 locations in standing waters, 500 locations in waters of the bream zone and 257 locations in waters of the barbel zone. Standing waters scored substantially different from running waters. Standing waters rarely contained no fish at all, but their fish communities were very often poor to very poor. Waters of the bream and barbel zone were often fishless (respectively 40% and 35% of all locations contain no fish), but the locations with fish usually scored reasonable to poor. Only 18.5% of all locations were classified as reasonable to excellent (IBI classes 4 or lower on a scale from 1 to 9) and were considered to satisfy the basic ecological quality demands. The Leie-, Dijle-, Dender- and Schelde-basins had a very poor quality (more than 50% of the locations contained no fish). The Maas-, Grote and Kleine Nete-basins scored rather well, with respectively, 44%, 48% and 68% of the locations achieving an IBI of 4 or lower. The IBI is a valuable and complementary tool to assess the ecological quality of water bodies as suggested in the proposal for a Water Framework Directive by the European Commission.
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  • 84
    ISSN: 1573-3025
    Keywords: aerobiology ; airborne pollen ; León (Spain) ; lower atmosphere ; meteorology ; mixing ratio ; relative humidity ; tethered balloon ; temperature ; wind speed
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract This study was undertaken in order to understand thebehaviour of airborne pollen grains, namely therelationship between their concentrations andconcomitant meteorological parameters, at differentaltitudes (ground level, 200, 400 and 600 m) of thelower atmosphere and its daily variations over onesite (the experimental farm of the University ofLeón). The experimental design involved a tetheredballoon (2.25 m3), an ADAS remote meteorologicalstation and an original radio controlled three headedpollen and spore sampler (called GABIS) using theRotorod design. Each head sampled a differentaltitude, while two control samples were taken atground level. Twenty-one takeoffs could be realized inthe 5 weeks period between end of May and end of June1997. Sampling was done early in the morning and sampling time at each altitudewas of 15 minutes. Results show that 45 differenttypes of pollen grains could be collected at this timeof the year and that significant variations could beobserved in the behaviour of the pollen cloud on a dayto day basis, probably because of differentmeteorological situations. Contrary to what isgenerally believed, pollen was in most cases moreabundant at higher altitude – on average by 30% ascompared to ground level – making evident anaerobiological layer of transport at about 500 m aboveground. This was especially the case for trees (Quercus and Castanea). The atmosphere MixingRatio was the most explicative factor at 200 m, whiletemperature dominated significance analysis at both400 and 600 m.
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  • 85
    ISSN: 1573-3017
    Keywords: fish ; chub ; vitellogenin ; environmental estrogens ; testis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Laboratory and field studies were carried out in order to assess, in a common cyprinid freshwater fish, the chub (Leuciscus cephalus), the feasibility of biological methods to detect and quantify estrogenic effects. In the laboratory the effects of 17beta estradiol were investigated on Vitellogenin (Vtg) induction and on testis organisation. Vtg was quantified by immunodetection using an established ELISA for carp (Cyprinus carpio) Vtg. Estradiol exposure resulted in a significant and rapid increase in plasma Vtg in both male and female chub, indicating that vitellogenic response in the chub is sensitive to estrogen(s). Histological examination of the testis in males exposed to estradiol also showed effects on the testis. Preliminary field studies on wild chub showed that fish living in a polluted river had elevated concentrations of plasma Vtg and had some alterations in the testis compared with wild fish from a reference site. Together these preliminary laboratory and field results show that chub could be an interesting fish species for further studies of biological effects of environmental estrogens in the aquatic environment.
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  • 86
    ISSN: 1573-2932
    Keywords: diffusion rate ; migration ; pH ; temperature ; total dissolved solids ; unplasticized PVC pipes ; vinyl chloride monomer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract The migration of vinyl chloride monomer (VCM) from unplasticizedpolyvinyl chloride (uPVC) pipes was investigated using locallymanufactured pipes. Specimens of 33 cm long were used throughoutthe research. The investigation was carried out under differentconditions of water temperature, pH and total dissolved solidsconcentration and at different durations of exposure. The VCMconcentration in the water was evaluated using the gaschromotography (GC)/head-space technique. A VCM concentration ofmore than 2.5 ppb was detected after 30 days of exposure at45 °C. The initial VCM concentration in the uPVC pipewas predicted using equations derived from Fick's first law ofdiffusion. Water tenperature did not affect the migration ofVCM, unless it was raised to high values (i.e. 45 °C).Total dissolved solids (TDS) and pH of water were found toaffect the release of VCM from uPVC pipes. Diffusion rate of VCMwas predicted as a function of pH or TDS values.
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  • 87
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    Environmental monitoring and assessment 60 (2000), S. 337-357 
    ISSN: 1573-2959
    Keywords: cellulose ; charcoal kiln ; decomposition ; herb seeds ; moisture ; respiration ; smoke pollution ; soil ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Long-term charcoal production in small private charcoal kilns (CK) in Eastern Bieszczady Mts. (SE Poland) can cause local smoke contamination of the ambient forest environment. Responses of model soil systems, contaminated or not contaminated by CK smoke, to contrasting combinations of hydrothermic regimes were compared in laboratory microcosms (respiration of soil community, decomposition rate of soil organic matter and cotton stripes, herb seeds germination were studied). The majority of the obtained data show a markedly higher level of soil biological activity in the CK versus the control series. In some cases CK and control soil systems show different patterns of reactions to the tested combinations of microclimate regime. These phenomena should at least partly be attributed to the effects of CK pollution.
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  • 88
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    Environmental monitoring and assessment 61 (2000), S. 207-229 
    ISSN: 1573-2959
    Keywords: benthos ; birds ; diatoms ; ecologicalindicators ; integrated assessment ; fish ; lakes ; monitoring ; zooplankton
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Biological indicators of communitiestypically reflect a common environmental signalreflecting the general condition of the ecosystem, as well asindividual signals by indicators differentiallysensitive to particular environmental conditions. Wedescribe here a method of integrating and interpretingsuch indicators from 19 New England lakes for fivetaxonomic groups (diatoms, benthos, zooplankton, fish,and birds). Our approach provides a systematicstandardized way to integrate multiple metrics fromdifferent taxonomic groups by addressing four elementscrucial to analyzing data from multiple indicators: covariate control, re-scaling of data, standardizing the sign of responses, and dimensional reduction. We evaluated the biologicalmetrics against individual environmental stressors andagainst multivariate physicochemical metricscharacterizing general anthropogenic stress among thelakes. The method detected a response to variationin the gross environmental condition of the lakes thatwas correlated across taxa and metrics. In addition,a differential response to near shore conditions wasdemonstrated for fish. The success of the approach inthis study lends support to its general application toecological monitoring involving complex data sets.
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  • 89
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    Environmental monitoring and assessment 61 (2000), S. 285-291 
    ISSN: 1573-2959
    Keywords: adsorption ; NO2 ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract The sodium arsenite method developed by Jacobs andHochheiser is one of the most widely used manualmethods for nitrogen dioxide (NO2) monitoring inambient air, particularly in developing countries. Asreported, the method gives 82% NO2 absorptionefficiency (NAE) in the concentration range from 40 to750 μg/m3, when only one impinger tube isemployed in the sampling train at a flow rate of 0.2lpm and for 24 h sampling duration. Accordingly,a uniform correction factor (0.82) is used indenominator to calculate the ultimate concentration ofNO2 in ambient air.In the present investigation, the effect oftemperature on absorption efficiency of NO2 isstudied employing four impinger tubes in series tocollect the maximum NO2 generated in the gasstream. The study conducted at 16, 26 and 36 °Ctemperatures shows maximum absorption efficiency(average) of 87.8% at 26°C in 1st impingertube. At lower and higher temperatures, it is foundconsiderably less. A suitable correction factor,therefore, must be applied to estimate actual NO2concentration in ambient air using arsenite method, intropical countries like India, where atmospherictemperature variations are large (less than 5°Cin winter and more than 45°C in summer).
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  • 90
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    Ecotoxicology 9 (2000), S. 115-125 
    ISSN: 1573-3017
    Keywords: phytoestrogens ; estrogenicity ; hepatocytes culture ; estrogen receptor affinity ; fish
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Soy as several other vegetables is rich in phytoestrogens and we demonstrated that these compounds were both estrogenic and endocrine disrupters, in sturgeon and trout, in vivo and in vitro. In our laboratory, we developed several in vitro tests in these two species to screen the activity of estrogenic compounds. This paper reports hepatocytes, which synthesise VTG in response to estrogenic stimulation, and in vitro binding tests on hepatic estrogen receptors. These tests showed different reactivities in the two species suggesting a difference of sensitivity, which is also observed in vivo. Finally, comparing the two species the in vitro affinity of phytoestrogens for estrogen receptors did not reflect the VTG synthesis in hepatocyte cultures. All these results, together with the tyrosine kinase inhibitory effect of certain phytoestrogens demonstrated by other authors, suggest that the stimulation of the VTG gene transcription may not be explained exclusively by a simple ligand-receptor-DNA interaction.
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  • 91
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    Studia geophysica et geodaetica 44 (2000), S. 442-459 
    ISSN: 1573-1626
    Keywords: temperature ; groundwater ; sedimentary basin ; exploitation ; transient effects
    Source: Springer Online Journal Archives 1860-2000
    Topics: Architecture, Civil Engineering, Surveying , Geosciences , Physics
    Notes: Abstract The subsurface temperature field was studied on a set of 46 borehole logs measured in the vicinity of uranium deposits in the Bohemian Cretaceous Basin. Vertical variations of the steady state temperature and the temperature gradient are governed by thermal conductivity which strongly varies in dependence on lithology. Large departures from undisturbed temperature detected in many holes are associated with uranium mining. A positive anomaly is observed in leaching fields where large amounts of acid are injected into the uranium-bearing Cenomanian. A negative anomaly is linked to the operation of hydraulic barriers which enclose the mining area and helps to contain pollution by pumping clean water into the Cenomanian aquifer. The spatial distribution of the observed temperature anomalies helps to map the migration of the fluids used in both processes. The temperature disturbance is propagated from the Cenomanian aquifer up through overlying impermeable sediments. The good fit of transient conductive models to the measured temperatures rules out heat advection and hence upward water flow towards and contamination of the upper Middle Turonian aquifer in the vicinity of the holes studied.
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  • 92
    ISSN: 1573-2932
    Keywords: microalgal communities ; photosynthesis ; temperature ; thermal pollution ; tropical coast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract The influence of thermal discharges on thephytoplankton community from a coastal zone of theGulf of Mexico was evaluated through their structureand photosynthetic behaviour focusing on responses tochanges in light and temperature. Biological andphysicochemical parameters were measured over a periodof two years in an area with permanent hot waterdischarges from a thermoelectric plant. Thetemperature in the sampling area ranged from 23.5 to36 °C with differences between the coldest andthe hottest station from 5.3 to 9.2 °C.Photosynthetically active radiation (PAR) were reducedin the discharge area water column, due to turbulence.One hundred and one different taxa were identifiedwith a strong predominance of Diatoms. The chlorophylla concentration ranged from 0.3 to 6.1 μgL-1, with highest values of thephaeophytin:chlorophyll ratio found at the hottest station.The community structure did not show significativedifferences among sampling stations with respect totemperature variations. However, in the algalassemblages influenced by thermal discharges, it waspossible to observe alterations in the photosynthesisbehaviour. Phytoplankton response to short termphotosynthesis experiments was segregated according tocomposition and origin of microalgal assemblages.Samples with larger heterogeneous composition had moreconsistent oxygen production responses. Algalcommunities exposed to hot effluent showed differentdegrees of photosynthesis rate reduction,higher light requirements (〉500 μE m2 s-1)and lower temperature (25 °C) to achieve Pmaxthan algae sampled in sites without such exposure.
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  • 93
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    Water, air & soil pollution 123 (2000), S. 337-352 
    ISSN: 1573-2932
    Keywords: bleaching ; bacterial infection ; coral ; Oculina ; temperature ; toxin ; zooxanthellae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract Bleaching in stony-corals is the result of disruption of symbiosis between the coral hosts and photosynthetic microalgal endosymbionts (zooxanthellae). Coral bleaching events of unprecedented frequency and global extent have been reported during the last two decades. Recently, we demonstrated that bleaching of the coral Oculina patagonica in the Mediterranean Sea is caused by the bacterium Vibrio shiloi, when seawater temperature rises and allows the bacterium to become virulent. The first step in the infection process is host-specific adhesion of V. shiloi to O. patagonica via a β-galactoside receptor on the coral surface. The bacterium then penetrates into the coral tissue and produces extracellular materials which rapidly inhibit photosynthesis of zooxanthellae and bleach and lyse the algae. The inhibition of pothosynthesis is due to a low molecular weight, heat stable toxin and ammonia. Bleaching and lysis are due to a heat-labile, high molecular weight materials, probably lytic enzymes. Elevated temperature induces different virulence factors within the infectious agent of the disease, V. shiloi. Adhesion was found to be temperature-regulated. When the bacteria were grown at 16°C there was no adhesion to corals maintained at either 25°C or 16°. However, when the bacteria were grown at 25°C they adhered avidly to corals maintained at 16°C and 25°C. In addition, the production of lytic enzymes and the photosynthesis inhibitor was also found to be temperature dependent. Production of the latter toxin was ten times greater at 29°C than at 16°C, and extracellular protease was 5-fold higher in cultures grown at 29°C than at 16°C. The data presented here suggest an explanation for the correlation between elevated seawater temperatures and seasonal coral bleaching.
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  • 94
    ISSN: 1573-2932
    Keywords: fish ; French Guiana ; mercury ; stream
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract This study examines the level of mercury contamination for different components of the biosphere in French Guiana. The spatial variability of the contamination of the waters, soils and sediments in the catchment area of the Sinnamary River is studied. The contamination of the trophic chain isdiscussed by analysis of the flesh of fish for the principal species represented in nine Guianese rivers, especially species consumed by human populations. The mercury contamination of sediments and soils, is extremely heterogeneous and that of fish is generalised. For carnivorous fish, the average mercury content is 0.48 μg g-1 (±0.28) (fresh weight) and for non-carnivorous species, 0.05 μg g-1 (±0.07). The concentrationsmeasured are greater than the European standards forconsumption for 4.7% of the carnivorous individuals.
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  • 95
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    Water, air & soil pollution 124 (2000), S. 301-317 
    ISSN: 1573-2932
    Keywords: fish ; lakes ; mercury ; model ; predictions ; regressions ; remedial measures
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract This work presents a new regression model for mercuryin lake fish, which provides the highest r 2-valueso far reported for a predictive model for Hg in lakefish. A new method to transform static regressionmodels to dynamic (time-dependent) models is alsopresented. The method to mathematically transformregression models to dynamic model uses the ecologicalhalflife concept and two differential equations, onefor the target organism (the predator) and one for theprey. This method is generic and can be applied inanalogous cases. The practical use of the dynamicmodel is illustrated in two case studies involvinglake liming and fertilization as methods to reduce Hgin lake fish. The dynamic model is a tool to obtainrealistic expectations of the recovery process, whichis slow because Hg in lake fish has a relatively longecological halflife (about 3 yr).
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  • 96
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    World journal of microbiology and biotechnology 16 (2000), S. 607-612 
    ISSN: 1573-0972
    Keywords: Aflatoxin ; apple ; fruit oils ; fungi ; patulin ; sodium hypochlorite ; temperature
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract Aspergillus flavus, A. niger, Penicillium expansum and Rhizopus stolonifer were the most frequently isolated fungi from healthy apple fruits. Alternaria alternata was the most common organism of rotten apple fruits, followed by A. niger, A. flavus, P. expansum and R. stolonifer. The prevalent type of decay, brown rot lesion, is caused by R. stolonifer followed by A. flavus, A. niger, A. alternata and P. expansum. Sodium hypochlorite had good curative properties against fruit rots. The main natural mycotoxins produced in rotten apple were patulin and aflatoxins. The optimum temperature for patulin production by P. expansum was 15 °C after 15 days. Complete inhibition of patulin formation was attained using 0.2% lemon oil and 〉 90% inhibition using 0.05% lemon and 0.2% orange oils. Also significant inhibition (〉 90%) of aflatoxin production was observed with 0.2% lemon oil.
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  • 97
    ISSN: 1573-2711
    Keywords: solid lubricants ; lubricious oxides ; TiO2 ; rutile ; oxygen stoichiometry ; Magnéli phases ; tribometry ; friction ; wear ; shear strength ; temperature ; atmosphere ; extreme environment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract In part I of this paper series, wide temperature range SEM-tribometric results generated in vacuum and various partial pressures of oxygen are combined with relevant literature data to examine a hypothesis correlating the oxygen stoichiometry of the Ti n O2n−1 Magnéli phases of the rutile polymorph of titania with their tribological behavior. Single-crystal and polycrystalline rutile specimens of narrow stoichiometry ranges were sliding against α-SiC and themselves. The surface shear strength changes were determined as a function of the thermal–atmospheric test environment, and the shear strength values were estimated by the coefficients of friction, the real area of contact and the published yield strength of rutile. The data appear to be sufficient and sufficiently reliable to confirm the accuracy of the hypothesis. The tendency of the rutile stoichiometry (ergo the friction) to shift as a function of temperature and partial pressure of oxygen causes this material to be thermo-oxidatively unstable for tribological applications in extreme environments. In part II, a study is described to formulate oxidatively more stable Magnéli phases by Cu-doping, and test the new materials by SEM tribometry using a procedure used for pure rutile here in part I. By employing this doping methodology similar to creating high-temperature superconductive oxides in part II, some feasibility of producing oxidatively stable, lubricious oxides with acceptably low wear rates is indicated.
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  • 98
    ISSN: 1573-2711
    Keywords: AFM ; force ; friction ; adhesion ; molecular water layer ; temperature ; capillary forces ; PMIRRAS
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract Under ambient conditions, a water film is always present on a silica substrate and generates additional capillary forces between the nanotip and the studied surface. In the present paper, we report AFM measurements of pull-off and friction forces as a function of the temperature and a comparative FTIR spectroscopy study. The AFM results show a net decrease of the forces as the temperature increases, while the IR spectroscopy indicates that the liquid film is removed at high temperature. Consequently, we deduce that a liquid neck is created between the tip and the surface and that the forces measured are mostly capillary forces. The present work shows that temperature studies with AFM can be a useful way to probe the influence of the capillary force in turn to characterize surface properties.
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  • 99
    ISSN: 1573-2711
    Keywords: solid lubricants ; lubricious oxides ; TiO2 ; rutile ; oxygen stoichiometry ; Magnéli phases ; tribometry ; friction ; wear ; shear strength ; temperature ; atmosphere ; extreme environment ; molecular engineering ; cation doping ; aliovalency
    Source: Springer Online Journal Archives 1860-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Notes: Abstract As a follow-up to the work described in part I of this paper series, a preliminary study was conducted with polycrystalline rutile TiO−x to render its friction and wear performance independent of the environment. The main goal was to confine the most tribologically desirable oxygen stoichiometries of the crystallographic shear-induced Magnéli phases (CSMP) of rutile by doping with cations similar in size and polarizability to the Ti4+, but with lower valences. The resultant chemical expulsion of oxygen from the rutile lattice was intended to generate CSMP free of friction and wear variations caused by changes in the thermal–atmospheric environment. Copper, iron, cobalt and nickel ions were tried as dopants introduced as their stable oxides via a simple ball-milling, hot-pressing and annealing procedure, but only a (Ti + Cu)O1.80 model blend resulted in a desired reaction. A portion of the copper entered the lattice to form a new titanium–copper CSMP, resembling the well-known V3Ti6O17 catalyst equivalent to an undoped rutile CSMP with an O/Ti ratio of 1.89. Although the shear behavior of this new, wear-resistant compound was in accordance with predictions, its friction is higher and more variable than desired for a wide environmental regime lubricant. The preparation technique was only sufficient to demonstrate that oxygen vacancy-induced creation of low-friction CSMP may be possible by doping; it does not appear to be useful for formulating practical, rutile-based lubricious oxides.
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  • 100
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    European journal of plant pathology 106 (2000), S. 77-85 
    ISSN: 1573-8469
    Keywords: Pestalotiopsis sydowiana ; morphology ; pathogenicity ; ericaceous plants ; conifers ; temperature ; pH ; water potential
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Pestalotiopsis isolates obtained from the foliage, stem-base and roots of hardy ornamentals grown on commercial nurseries in the UK were identified and characterised according to pathogenicity and colony morphology. All 18 isolates were identified as Pestalotiopsis sydowiana on the basis of conidia morphology, and confirmation of identification was made by experts at CABI Bioscience. Isolates were pathogenic on the host from which originally isolated. Typical symptoms included foliar browning of foliage and stems, and the presence of black or greenish-black acervuli on diseased tissue. Isolates were not host specific and infected other species of hardy ornamentals. Three colony types on potato dextrose agar were distinguished according to colour and production of acervuli by individual isolates. Three selected isolates of P. sydowiana were characterised by examining the effects of growth media, temperature, pH, and water potential on hyphal extension. Isolates grew well on commonly used growth media, including PDA, Sabouraud dextrose agar (SDA), V8 juice agar (V8), malt extract agar (MEA) and Czapek Dox agar (CDA). The optimum temperature for growth on PDA was in the range 20–25°C, with little or no growth occurring below 5°C or above 30°C. Hyphal extension occurred over a pH range between 2.6–8.6, with optimum values occurring at pH 5.5. In general, decreases in osmotic and matric potential caused a reduction in growth. Hyphal extension on media adjusted osmotically as NaCl ceased between −9.9 and −10.5 MPa. Isolates were more tolerant of osmotic than matric potential, with no growth occurring at −6.5 MPa on media adjusted with polyethylene glycol.
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