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In vitro Estrogenic Effects of Phytoestrogens in Rainbow Trout and Siberian Sturgeon

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Abstract

Soy as several other vegetables is rich in phytoestrogens and we demonstrated that these compounds were both estrogenic and endocrine disrupters, in sturgeon and trout, in vivo and in vitro. In our laboratory, we developed several in vitro tests in these two species to screen the activity of estrogenic compounds. This paper reports hepatocytes, which synthesise VTG in response to estrogenic stimulation, and in vitro binding tests on hepatic estrogen receptors. These tests showed different reactivities in the two species suggesting a difference of sensitivity, which is also observed in vivo. Finally, comparing the two species the in vitro affinity of phytoestrogens for estrogen receptors did not reflect the VTG synthesis in hepatocyte cultures. All these results, together with the tyrosine kinase inhibitory effect of certain phytoestrogens demonstrated by other authors, suggest that the stimulation of the VTG gene transcription may not be explained exclusively by a simple ligand-receptor-DNA interaction.

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Authors and Affiliations

  1. Laboratoire de Biologie de la Reproduction des Poissons, UA INRA, Université Bordeaux I, avenue des Facultés, 33405, Talence cedex, France

    K. Latonnelle

  2. Laboratoire de Biologie de la Reproduction des Poissons, UA INRA, Université Bordeaux I, avenue des Facultés, 33405, Talence cedex, France

    F. Le Menn & C. Bennetau-Pelissero

  3. ENITA de Bordeaux, 1, cours du Général de Gaulle, 33175, Gradignan cedex, France

    C. Bennetau-Pelissero

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  1. K. Latonnelle
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  2. F. Le Menn
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  3. C. Bennetau-Pelissero
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Latonnelle, K., Le Menn, F. & Bennetau-Pelissero, C. In vitro Estrogenic Effects of Phytoestrogens in Rainbow Trout and Siberian Sturgeon. Ecotoxicology 9, 115–125 (2000). https://doi.org/10.1023/A:1008932632135

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