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  • taxonomy  (63)
  • Triticum aestivum
  • Springer  (100)
  • American Physical Society
  • MDPI Publishing
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  • 1965-1969
  • 1935-1939
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  • 1
    ISSN: 1439-0361
    Keywords: mate choice ; taxonomy ; phenotypic hybrids ; fitness ; decision rule
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Description / Table of Contents: Zusammenfassung Die als Unterarten klassifizierten europäischen Formen der Aaskrähe, Rabenkrähe und Nebelkrähe, besiedeln verschiedene, aneinandergrenzende Verbreitungsgebiete und hybridisieren in der Kontaktzone. Die Nachkommen von Mischpaaren sind fruchtbar und können sowohl mit anderen Hybriden als auch mit Raben- und Nebelkrähen erfolgreich brüten. Trotzdem kommt es zu keiner völligen Vermischung der Formen und/oder Verlagerung der Verbreitungsgebiete. Vor diesem Hintergrund untersuchten wir die Partnerwahl von Aaskrähen in der Hybridisierungszone auf der nordfriesischen Insel Amrum und stellten fest, daß Partner gleichen Phänotyps häufiger miteinander verpaart waren, als stochastisch zu erwarten gewesen wäre. Unsere Daten bestätigen vergleichbare Studien aus Hybridisierungszonen in Italien und Sibirien. Wir schließen daraus, daß phänotypisch selektive Partnerwahl bei der Aaskrähe ein allgemeines Phänomen sein könnte und diskutieren, warum dieses Verhalten anfitness-relevante Parameter gekoppelt sein sollte. Um welche es sich dabei handeln könnte, wurde bisher nicht hinreichend untersucht und muß deshalb offen bleiben.
    Notes: Summary Carrion Crow and Hooded Crow are regarded as subspecies of the Crow. They show frequent hybridisation along the adjacent borders of their distribution. Mixed pairs produce fertile offspring which are able to breed successfully with both hybrids and mates of either phenotype. However, hybridisation does not lead to phenotypic changes of Carrion and Hooded Crows in general nor in their distinct distribution. We studied the mating behaviour of Crows in the hybrid zone on the Island of Amrum (Schleswig-Holstein, Germany) and found evidence that Crows may prefer mates of the same phenotype. Our data confirm previous studies which reported assortative mating with respect to plumage coloration from hybrid zones in Italy and Siberia. We discuss why this behaviour should be related tofitness traits which in our opinion have not yet been studied adequately nor identified.
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  • 2
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    Antonie van Leeuwenhoek 74 (1998), S. 229-235 
    ISSN: 1572-9699
    Keywords: Dipodascus capitatus ; D.spicifer ; Geotrichum clavatum ; yeast ; taxonomy ; DNA heterogeneity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The G+C contents of 25 strains of Dipodascus capitatus, Dipodascus spicifer and Geotrichum clavatum were found to be heterogeneous on basis of derivative graphs of the melting profiles. Strains showing similar derivative graphs of the melting curve exhibited high levels of DNA homology (80-100%); strains showing dissimilar derivative graphs exhibited low levels of DNA homology (5 to 45%). Being considered separate taxa on basis of these parameters, D. capitatus, D. spicifer and G. clavatum could be identified by a combination of the key characteristics growth on xylose, cellobiose, salicin and arbutin.
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  • 3
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    Plant molecular biology reporter 16 (1998), S. 147-155 
    ISSN: 1572-9818
    Keywords: allele-specific PCR ; Beta ; ITS1 ; plant identification ; rDNA ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Sequence variation in the ITS1 locus of the nuclear ribosomal DNA in beets has previously been used to reconstruct phylogeny of the species in the genus Beta. We have developed protocols that allow the identification of Beta taxa by use of taxon-specific primers. Beta sections, species and subspecies can be identified. Differences within the ITS1 region of a single base can be exploited for species identification. The results from this study not only provide effective methods for wild beet identification, but also indicate the potential use of the techniques in other crops.
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  • 4
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    Journal of insect conservation 2 (1998), S. 151-159 
    ISSN: 1572-9753
    Keywords: Orthoptera ; biodiversity ; taxonomy ; conservation.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Nature of Science, Research, Systems of Higher Education, Museum Science
    Notes: Abstract It is estimated that only 10–15% of the world's insect fauna has been described and named. Efforts to inventory insect biodiversity are hampered by this taxonomic impediment, which is compounded by the logistical problems of an insufficient taxonomic workforce and their remote location in museums thousands of miles from the areas of highest biodiversity. Compared to most other invertebrate groups however, the taxonomic impediment is relatively benign in the order Orthoptera. This is a small to medium-sized order (approximately 20 000 described species) which is well known taxonomically, owing to the group's agricultural importance worldwide. Furthermore, orthopteran taxonomists are now fortunate to have a published up-to-date catalogue of all known species, which has just become accessible as a regularly updated database on the World Wide Web. Whilst new information technology, in the form of e-mail networks, World Wide Web sites and CD-ROM information archives, is already enhancing communication between specialists and helping to reduce the logistical problems of documenting orthopteran biodiversity, a major reinvestment in basic taxonomic research is needed if we are to reduce the existing taxonomic impediment significantly. There is general agreement that an internationally coordinated approach will be necessary and priorities must be set to tackle the biodiversity/systematics crisis. In the future, the Orthoptera can make an important contribution to invertebrate faunal surveys and have potential as an indicator taxon. Furthermore, the Orthoptera Species File establishes a taxonomic framework which could be readily enlarged to include geographic data and phenology of species from existing museum specimens.
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  • 5
    ISSN: 1573-3017
    Keywords: contamination risks ; fish ; Mississippi River ; ecological factors ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Energy, Environment Protection, Nuclear Power Engineering
    Notes: Abstract We compared contamination levels in fish from contaminated and uncontaminated floodplain swamps of the lower Mississippi River to assess differences in contamination risks between swamps, across different taxonomic and ecological groupings of fishes within and between swamps, and with seasonality in river stage. Fish tissue levels of inorganic contaminants were substantially lower than environmental levels in both swamps, suggesting either that fish were not uptaking these contaminants, or they were effectively eliminating the contaminants from their bodies. Tissue levels of organic contaminants were high relative to environmental levels, suggesting that these contaminants were bioaccumulating. Organic contaminants were significantly higher in fish from the contaminated swamp (Devil's Swamp) than in fish from a reference swamp up river (Tunica Swamp). Because the organic contaminants were largely confined to sediments, we expected bottom-oriented fishes to have higher concentrations than pelagic fishes. Assuming that uptake was primarily through the food chain, we expected top predators to exhibit higher concentrations than low-level consumers. We also expected year- round swamp residents to exhibit higher accumulations than more transitory users of backswamp habitat. However, organic contaminant levels did not differ in the directions expected for any of these groupings. We did observe differences in organic contaminant levels within and between swamps for different taxonomic groupings of fishes (species and genera). Some taxa occupying low to middle positions in the food web (e.g., gizzard shad, Lepomis spp.) exhibited higher concentrations than taxa near the top of the food web. Within Devil's Swamp, organic contaminant levels were significantly higher at low river stage, when fish were confined to the swamp, than at high river stage, when fish were free to move between the river and the swamp. We caught more species and more fish per unit effort in Devil's Swamp than in Tunica Swamp, contrary to expectations if contaminants in the former were negatively impacting population and community structure. Species richness differences between swamps were a consequence of catch differences, with higher catch corresponding to inclusion of more rare species. The lower catch in Tunica Swamp may have resulted from physical modifications of its waterways to support agriculture and hunting. The results of this study underscore the importance in factoring information on the taxonomy and ecology of organisms, and seasonal changes in environmental conditions, into assessments of contamination risks.
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  • 6
    ISSN: 1432-203X
    Keywords: Key words Bread wheat ; Triticum aestivum ; Tritordeum ; Tissue electroporation ; Transient gene expression
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Wheat scutella and tritordeum inflorescences were transformed by tissue electroporation with plasmid DNA containing a β-glucuronidase (GUS) gene (gus A) under the control of the rice actin1 promoter. Factors affecting electroporation efficiency were analysed. Important factors were electroporation voltage and pulse length, the volume of electroporation buffer, the osmoticum of electroporation buffer and medium, the osmoticum of pre-electroporation culture medium, and pre-electroporation incubation time and temperature. Maximum transient gene expression was obtained with a single pulse of 550 V/cm from a 960-μF capacitor, using 200 μl of electroporation buffer, after 2–3 h culture on media with 357 mOsm for wheat scutella or 1 day on media with 222 mOsm for tritordeum inflorescences, and 0.5–1 h pre-electroporation incubation with DNA at 24 °C. Under these conditions, up to 90% of the explants showed GUS expression, and up to 149 expression signals were recorded per replicate. Electroporated explants showed high rates of survival and retained the ability to regenerate plants via somatic embryogenesis.
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  • 7
    ISSN: 1432-2242
    Keywords: Key words Aegilops markgrafii ; Triticum aestivum ; Addition lines ; Chromosome markers ; Homoeology ; Wheat ; Wheat microsatellites
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  We describe the use of wheat microsatellites for the discrimination of Aegilops markgrafii chromosomes. Twenty out of eighty eight wheat microsatellites (WMS) tested were able to distinguish Triticum aestivum-Ae. markgrafii addition lines. Six, three, three, one and six of 18 WMS can be used as markers for single Ae. markgrafii chromosomes B, C, D, F and G, respectively. Addition line A is not available but additional bands, appearing only in Ae. markgrafii and the T. aestivum-Ae. markgrafii amphiploid and not in any of the available addition lines, indicate that three WMS detect markers for Ae. markgrafii chromosomes A. Addition line E could not be detected by any of the WMS markers applied, although the 20 WMS represented all the homologous groups of wheat. All three WMS located on the short arm of group-2 chromosomes were located on Ae. markgrafii chromosome B; three of four WMS, located on the long arm of wheat group-2 chromosomes, were specific to Ae. markgrafii chromosome G and three of four WMS, specific to group-5 chromosomes, were markers for Ae. markgrafii chromosome C, indicating the homoeology of these wheat chromosome arms with the respective Ae. markgrafii chromosomes.
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  • 8
    ISSN: 1432-2242
    Keywords: Key words RFLP analysis ; Triticum aestivum ; Genetic diversity ; Genetic similarity estimates ; Cluster analysis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Restriction fragment length polymorphisms (RFLPs) have been used to characterise the genetic diversity of wheat (Triticum aestivum) germplasm. One hundred and twenty-four accessions comprising all major Australian wheat varieties and lines important for breeding purposes were assayed for RFLPs with clones of known genetic location and selected to give uniform genome coverage. The objectives of this study were to determine RFLP-based genetic similarity between accessions and to derive associations between agronomically significant traits and RFLP phenotypes. Ninety-eight probes screened against genomic DNA digested with five restriction endonucleases detected a total of 1968 polymorphic fragments. Genetic similarity (GS) calculated from the RFLP data ranged from 0.004 to 0.409 between accessions, with a mean of 0.18. Cluster analysis based on GS estimates produced four groupings that were generally consistent with available pedigree information. Comparisons of the RFLP phenotypes of accessions containing disease resistance genes present on introgressed alien segments enabled the identification of specific alleles characteristic of these regions. Associations were derived for a range of stem-rust, leaf-rust and yellow-rust resistance genes. These results suggest that RFLP analysis can be used for the characterisation and grouping of elite breeding material of wheat and RFLP profiling can identify chromosome segments associated with agronomic traits.
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  • 9
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    Theoretical and applied genetics 96 (1998), S. 69-75 
    ISSN: 1432-2242
    Keywords: Key words Aegilops umbellulata ; Co-linearity ; Comparative mapping ; Translocations ; Triticum aestivum ; Wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  A comparative genetic map of Aegilops umbellulata with wheat was constructed using RFLP probes that detect homoeoloci previously mapped in hexaploid bread wheat. All seven Ae. umbellulata chromosomes display one or more rearrangements relative to wheat. These structural changes are consistent with the sub-terminal morphology of chromosomes 2 U, 3 U, 6 U and 7 U. Comparison of the chromosomal locations assigned by mapping and those obtained by hybridization to wheat/Ae. umbellulata single chromosome addition lines verified the composition of the added Ae. umbellulata chromosomes and indicated that no further cytological rearrangements had taken place during the production of the alien-wheat aneuploid lines. Relationships between Ae. umbellulata and wheat chromosomes were confirmed, based on homoeology of the centromeric regions, for 1 U, 2 U, 3 U, 5 U and 7 U. However, homoeology of the centromeric regions of 4 U with wheat group-6 chromosomes and of 6 U with wheat group-4 chromosomes was also confirmed, suggesting that a re-naming of these chromosomes may be pertinent. The consequences of the rearrangements of the Ae. umbellulata genome relative to wheat for gene introgression are discussed.
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  • 10
    ISSN: 1432-2242
    Keywords: Key words Fluorescence in situ hybridization ; Translocation ; WSMV resistance ; Thinopyrum intermedium ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract   Thinopyrum intermedium is a promising source of resistance to wheat streak mosaic virus (WSMV), a devastating disease of wheat. Three wheat germplasm lines possessing resistance to WSMV, derived from Triticum aestivum×Th. intermedium crosses, are analyzed by C-banding and genomic in situ hybridization (GISH) to determine the amount and location of alien chromatin in the transfer lines. Line CI15092 was confirmed as a disomic substitution line in which wheat chromosome 4A was replaced by Th. intermedium chromosome 4Ai?2. The other two lines, CI17766 and A29-13-3, carry an identical Robertsonian translocation chromosome in which the complete short arm of chromosome 4Ai?2 was transferred to the long arm of wheat chromosome 4A. Fluorescence in situ hybridization (FISH) using ABD genomic DNA from wheat as a probe and S genomic DNA from Pseudoroegneria stipifolia as the blocker, and vice versa, revealed that the entire short arm of the translocation was derived from the short arm of chromosome 4Ai?2 and the breakpoint was located at the centromere. Chromosomal arm ratios (L/S) of 2.12 in CI17766 and 2.15 in A29-13-3 showed that the translocated chromosome is submetacentric. This translocated chromosome is designated as T4AL ⋅ 4Ai?2S as suggested by Friebe et al. (1991).
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  • 11
    ISSN: 1432-2242
    Keywords: Key words Triticum spelta ; Triticum aestivum ; SDS-PAGE ; Acid-PAGE ; Seed storage proteins
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Seed storage proteins of a few selected spelt forms and crosses have already been electrophoretically analysed by SDS-PAGE and acid-PAGE and compared with a few winter wheat cultivars. In the analyses presented here further important Central European spelt varieties were included, as well as modern winter wheat cultivars which were chosen as standards. In this study gliadin and glutenin band patterns of modern Central European winter wheat cultivars were analysed, in particular for a comparison with spelt varieties. An improved differentiation within and between the two species was obtained.
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  • 12
    ISSN: 1432-2242
    Keywords: Key words Genetic diversity ; Triticum tauschii ; Triticum aestivum ; RFLP ; Landrace wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Chinese accessions of Triticum tauschii and T. aestivum L. from the Sichuan white (SW), Yunnan hulled (YH), Tibetan weedrace (TW), and Xinjiang rice (XR) wheat groups were subjected to RFLP analysis. T. tauschii and landraces of T. aestivum from countries in Southwest Asia were also evaluated. For T. tauschii, a west to east gradient was apparent where the Chinese accessions exhibited less diversity than those from Southwest Asia. Compared to the Southwest Asian gene pool, the Chinese T. tauschii was highly homogeneous giving a low frequency of polymorphic bands (16%) and banding patterns (1.33 per probe) with 75 RFLP probe-HindIII combinations. Accessions of T. tauschii from Afghanistan and Pakistan were genetically more similar to the Chinese T. tauschii than those from Iran. Of 368 bands found for 39 Chinese hexaploid wheat accessions with 63 RFLP probe-HindIII combinations, 28.3% were polymorphic with an average of 2.6 banding patterns per probe and 5.0 bands per genotype. The individual Chinese landrace wheat groups revealed less variation than those from Afghanistan, Iran, and Turkey. When classified into country based groups, however, the diversity level over all Chinese landraces was greater than that of some Southwest Asian landraces, especially those from Afghanistan and Iran . The XR wheat group was genetically distinct from the other three Chinese landrace groups and was more related to the Southwest Asian landraces. The TW group was genetically similar to, but more diverse than, the SW and YH groups. The Chinese landraces had a higher degree of genetic relatedness to the Southwest Asian T. tauschii, particularly to accessions from Iran, rather than to the Chinese T. tauschii. ‘Chinese Spring’ was most related to ‘Chengdu-guang-tou’, a cultivar from the SW wheat group.
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  • 13
    ISSN: 1432-2242
    Keywords: Key words Aegilops triuncialis ; Triticum aestivum ; Heterodera avenae ; Cereal cyst nematode ; Resistance gene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The cereal cyst nematode (Heterodera avenae) is an important root parasite of common wheat. A high level of resistance was transferred to wheat from Aegilops triuncialis (TR lines) using the cross [(T. turgidum×Ae. triuncialis)×T. aestivum]. Low fertility (3–5 viable kernels per plant) was observed during the process but the surviving hybrid plants were highly vigorous. To obtain stable resistant lines further crosses to T. aestivum were performed. The resistance in TR lines seems to be transferred from the C genome of Ae. triuncialis (genomes CCUU). Ae. triuncialis was highly resistant to the two Spanish populations of H. avenae tested, as well as to four French races and two Swedish populations. The histological analysis showed a hypersensitive reaction in the roots of a resistant TR line inoculated with the Ha71 pathotype of H. avenae, whereas well-formed syncytia were observed in the roots of the susceptible control. Resistance to the H. avenae Ha71 pathotype seemed to be inherited as determined by a single dominant factor in the crosses between resistant TR lines and susceptible cultivars.
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  • 14
    ISSN: 1573-9368
    Keywords: particle bombardment ; transgene expression ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A population of R0 transgenic wheat plants, generated by particle bombardment, was analyzed to define molecular, genetic and phenotypic properties resulting from transformation with a cointegrate vector, or cotransformation with two separate plasmids. By evaluating the progeny of 70 independently-derived transgenic plants, we also identified rare events such as chimerism and transgene elimination, which provide valuable information concerning the development of transgenic cereal plants following bombardment experiments. The frequency of chimerism in our transgenic wheat plants was very low. Furthermore, while transgene elimination did occur, this was also a very rare event. We determined the copy numbers of integrated transgenes and the levels of transgene expression. Comparisons to transgenic rice plants generated in the same manner demonstrated some similarities, but also important differences in transgene behavior. Whereas in rice there is no evidence for any direct relationship between transgene copy number and transgene expression or stability, multicopy populations in wheat demonstrated a bias towards higher levels of expression for the two genes and the maize ubiquitin promoter evaluated in the present study.
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  • 15
    ISSN: 1618-2545
    Keywords: Aphyllophorales ; ribosomalDNA ; synnematous hyphomycete ; taxonomy ; Tretopileus sphaerophorus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Tretopileus sphaerophorus, a synnematous hyphomycete with basidiomycetous affinities was newly isolated from the decaying petiole and peduncle ofCocos nucifera collected in Depok, Indonesia. The species produced first a bulbil as a propagule on the top of a synnema. After the bulbil had fallen, the synnema proliferated about seven times to produce new bulbils, each time making conspicuous nodes at the upper part. By careful morphological observation, clamp connections were confirmed on the hyphae in the specimens and culture. In culture, each hyphal cell with or without a clamp was found to be dikaryotic by DAPI nuclear staining. Germination of the bulbils occurred first from projecting hyphal tips on their upper surface, which have been treated as germ pores. The inner structure of the bulbils, the hyaline mucus of the bulbils, and conidium-like hyphal fragments were also examined. Phylogenetically,T. sphaerophorus was inferred to be related to the Aphyllophorales based on the nuclear encoded small subunit (18S) rDNA using the homology search system (FASTA) and the neighbour-joining method.
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  • 16
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    Mycoscience 39 (1998), S. 97-104 
    ISSN: 1618-2545
    Keywords: Loculoascomycetes ; phyllachoraceae ; phyllachorales ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The order Phyllachorales contains ascomycetous fungi of considerable economic importance. The group is represented mostly by foliar parasites which produce perithecia under a clypeus, inside a stroma, or do not produce any stromatic tissue. A major taxonomic problem with this order is the lack of reliable morphological characters that clearly delimit the entire group. The main purpose of this review is to provide a clear picture of the taxonomic relationships of the order Phyllachorales, along with a key to the most important genera in the family Phyllachoraceae.
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  • 17
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    Mycoscience 39 (1998), S. 31-36 
    ISSN: 1618-2545
    Keywords: Mycogloea ; Platygloea ; Platygloeaceae ; Platygloeales ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Three new species ofMycogloea are described and illustrated; they are:M. amethystina from Canada,M. nipponica, from Japan, andM. bullata from Thailand.Mycogloea tahitiensis is reported from Japan and additional undescribed taxa in the genus are briefly noted. Some characteristics of the genus are discussed, and a key is provided for six species recognized at this time.
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  • 18
    ISSN: 1615-6110
    Keywords: Fabaceae ; Vicia faba ; trnL intron ; PCR-sequencing ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The chloroplasttrnL intron from 46 differentVicia accessions, representing five of the nine sections of the genusVicia subg.Vicia sensuMaxted (1991a) were amplified by the Polymerase Chain Reaction (PCR) using oligonucleotide primers homologous to conserved regions intrnL. The products fell into two distinct groups; those of approximately 250 nt and those of around 450 nt in length. Of these, products from 17 differentVicia species were cloned and their nucleotide sequences determined. Multiple alignments were assembled and phylogenetic trees constructed by the weighted least-squares distance method. ALathyrus latifolius trnL intron sequence was used as an outgroup. The resulting trees clearly group and separate the sectt.Narbonensis, Bithynica andFaba species but were less able to distinguish species from sectt.Hypechusa andPeregrinae. Based on these sequence data,V. faba appears to be more distant from sect.Narbonensis than sectt.Hypechusa andPeregrinae. The results are in general agreement with a recent treatment ofVicia subg.Vicia (Maxted 1993) and lend further support to placingV. faba in the monospecific sect.Faba.
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  • 19
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    Plant systematics and evolution 213 (1998), S. 173-186 
    ISSN: 1615-6110
    Keywords: Fabaceae ; Vicia ; sect.Bithynicae ; sect.Narbonensis ; Allozymes ; genetic diversity ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Electrophoretic analysis of five enzyme systems, LAP, PGI, SKDH, SOD and 6-PGDH, among 102Vicia accessions representingV. bithynica and seven species of theV. narbonensis complex, namelyV. eristalioides, V. kalakhensis, V. johannis, V. galilaea, V. serratifolia, V. narbonensis andV. hyaeniscyamus, has been performed. The recorded variation was tentatively assigned to 41 allelic genes at eight loci; intraspecific variation was observed in all species except forV. eristalioides. The results obtained were compared with the corresponding data reported earlier forV. faba. Hierarchical grouping of the investigated taxa, includingV. faba, was based onNei's genetic identities calculated from the allozyme frequency data.Vicia faba andV. bithynica were shown to be most distantly related to one another and to the remaining species investigated.Vicia serratifolia appeared to be a peripheral member of theV. narbonensis complex. The results are discussed with reference to genetic diversity and taxonomic relationships of the species under study.
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  • 20
    ISSN: 1573-5176
    Keywords: Dunaliella ; ITS ; PCR ; RFLP ; strains ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The genus Dunaliella comprises 28 species defined primarily by morphological and physiological criteria, which vary considerably depending on growth conditions. Concomitantly, the taxonomic status of various species is uncertain. To confirm the taxonomic identity and to better understand the relationship within Dunaliella, seven taxa ( D. salina, D. bardawil, D. tertiolecta, D. parva, D. viridis, D. lateralis, D. peircei) were compared using RFLP analysis of the nuclear rDNA repeats, specifically the internal transcribed spacer regions, including the 5.8S rRNA gene. Volvox aureus was used as an outgroup. A single ITS PCR amplification product was obtained for each taxon. An ITS fragment of ca. 640 bp was present in all the taxa within the subgenus Dunaliella, except for D. salina CCMP 1303 (ca. 540 bp) and D. lateralis (subgenus Pascheria) (ca. 600 bp). A cluster analysis based on the presence or absence of bands generated by digestion of the PCR product with 8 restriction endonucleases (DpnI, HhaI, EcoRI, PvuII, TaqI, HaeIII, MspI, StyI) revealed no correlation between the genetic relationship inferred from the ITS-RFLP data and the morpho-physiological attributes used for taxonomy. In addition, differences in morphology, physiology and in the length and restriction fragment patterns of the ITS region of D. salina CCMP 1303 suggest that this strain does not belong to Dunaliella.
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  • 21
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    Mycoscience 39 (1998), S. 333-335 
    ISSN: 1618-2545
    Keywords: Cortinarius prunicola ; Cortinarius Sect.Sericeocybe ; new species ; Rosaceae ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cortinarius prunicola sp. nov., found in orchards and plantations ofPrunus mume, is described and illustrated. It is characterized by its dry and violet-white carpophores, unpleasant odor, and its close association withP. mume in spring and early summer. The differences betweenC. prunicola and similar species are briefly discussed.
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  • 22
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    Journal of applied phycology 10 (1998), S. 237-251 
    ISSN: 1573-5176
    Keywords: Gelidiales ; Gelidiella ; Gelidium ; Pterocladia ; Pterocladiella ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Segregating Pterocladiella from Pterocladia stimulated new taxonomic studies of the species originally assigned to Pterocladia. A total of 28 species are ascribed to the genus, one of them with doubts. Thirteen of the 27 names are synonyms. Three of the remaining 14 species belong with Gelidium, including G. americanum, G. mcnabbianum (Dawson) comb. nov. and G. musciformis. Seven other species belong with Pterocladiella, including P. bartlettii (Taylor) comb. nov., P. bulbosa, P. caerulescens, P. caespitosa (Kylin) comb. nov., P. caloglossoides (Howe) comb. nov., P. capillacea and P. melanoidea. Two species are retained in Pterocladia, the type P. lucida and P. rectangularis. Two others, P. heteroplatos and P. media are placed in incertae sedis as additional studies of fertile materials are needed to determine their generic status. The genus Pterocladia now appears to include two large-sized species restricted to Australia-New Zealand. Pterocladiella has 8 small-sized species (including P. minima), mainly inhabiting tropical and subtropical waters. Future research documenting sexual reproduction in Gelidiella and solving the presently recognized heterogeneity in Gelidium will help to trace the relationships between Pterocladiella and these two genera.
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  • 23
    ISSN: 1573-8469
    Keywords: citrus bacterial canker ; detection ; epidemiology ; monoclonal antibodies ; variability ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Strains of Xanthomonas axonopodis pv. citri were isolated from Mexican lime (Citrus aurantifolia) trees in several countries in southwest Asia. These strains produced typical erumpent bacterial canker lesions on Mexican lime but not on grapefruit (C. paradisi). Lesions on grapefruit were watersoaked and blister-like in contrast to the typical erumpent lesions seen after artificial inoculation with all described pathotypes of X. axonopodis pv. citri. This group of strains hydrolysed gelatin and casein and grew in the presence of 3% NaCl as is typical of X. axonopodis pv. citri pathotype A. RFLP analyses and DNA probe hybridization assays also gave results consistent with X. axonopodis pv. citri pathotype A. Metabolic fingerprints prepared with the Biolog® system showed similarities as well as differences to X. axonopodis pv. citri pathotype A. In spite of the physiological and genetic similarities to pathotype A of X. axonopodis pv. citri, these strains had no or very little affinity for polyclonal antiserum prepared against any of the reference strains of X. axonopodis pv. citri and also did not react with monoclonal antibody A1, an antibody that detects all strains of pathotype A of X. axonopodis pv. citri. These strains were also insensitive to bacteriophage Cp3 like X. axonopodis pv. citri pathotype A and unlike X. axonopodis pv. citri pathotype B. We conclude that these strains, designated Xcc-A*, represent a variant of X. axonopodis pv. citri pathotype-A with pathogenicity limited to C. aurantifolia. The existence of extensive genotypic and phenotypic variation within pathotype A of X. axonopodis pv. citri was unexpected and further complicates the systematics of this species.
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  • 24
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    Hydrobiologia 369-370 (1998), S. 363-372 
    ISSN: 1573-5117
    Keywords: Euglenophyta ; South America ; geographical distribution ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A taxonomic revision of the Euglenophyta found exclusively in South America is presented. The taxonomy, geographical distribution and critical analysis of the synonyms are given. More than two hundred species and infraspecific entities from South America are described, many of them recorded several times in different regions. The genus Trachelomonas comprises the highest number of new taxa, reaching more than 100 entities. It is followed by Phacus (48 taxa), Strombomonas (33 taxa), Euglena (15 taxa) Lepocinclis (14 taxa), and Distigma, Entosiphon and Sphenomonas with one new taxon each.
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  • 25
    ISSN: 1573-5117
    Keywords: Neocervinia itoi ; Copepoda ; Harpacticoida ; taxonomy ; cold-seep ; Japan
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    Topics: Biology
    Notes: Abstract A new species of harpacticoid copepod, Neocervinia itoi (Cerviniidae), is described on the basis of females and copepodids collected from the Hatsushima cold-seep site in Sagami Bay, Japan. It is morphologically very close to its deep-sea congeners N. tenuicauda (Brotskaya, 1963) and N. unisetosa (Montagna, 1981). The new species differs primarily in the segmentation of the antennule and the endopod of both mandible and maxilliped, and in form and shape of the setae on leg 5. The presence of sensillar structures on the proximal part of the antennule is discussed. A key to the species of Neocervinia is presented.
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  • 26
    ISSN: 1573-5028
    Keywords: calcium ; in situ hybridization ; multigene family ; polyploid ; signal transduction ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Recently, we reported on the characterization of the calmodulin (CaM) gene family in wheat [44]. We classified wheat CaM genes into four subfamilies (SFs) designated SF-1 to SF-4, each representing a series of homoeoallelic loci on the homoeologous chromosomes of the three genomes of common wheat. Here we studied the expression of these wheat CaM genes in the course of wheat development. Northern blot analysis using SF-specific probes revealed differences in SF expression levels in different organs and stages of development. Subsequently, cell-specific expression of CaM SFs was investigated by in situ RNA hybridization. In developing seeds, all CaM SFs showed highest expression in the embryo and less in the aleurone and in the starchy endosperm. In primary roots, all four CaM SFs were expressed in the root cap, meristematic regions and in differentiating cells. During development of the roots, expression gradually decreased. The wheat glutenin gene, which was used as a control throughout our experiments, was found to be expressed in the starchy endosperm but not in the aleurone, embryos or vegetative tissues. In stems, at advanced stages of growth, differences in cell-specific expression of CaM SFs were found. For example, SF-2 was highly expressed in differentiating phloem fibers. Thus, CaM genes in common wheat exhibit a developmentally regulated organ-, tissue-, cell- and SF-specific expression patterns.
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  • 27
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    International journal of primatology 19 (1998), S. 879-905 
    ISSN: 1573-8604
    Keywords: Alouatta seniculus ; taxonomy ; geographic distribution ; Guianas
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Cytogenetic and morphological studies have begun to reexamine the taxonomy of the red howlers Alouatta seniculus which live throughout the northern and western Amazon basin, in the Guianas, and from northern Colombia and Venezuela, south to Bolivia. We briefly review the current state of knowledge of the taxonomy and distributions of red howlers, in particular, that of the Guianan subspecies. Recently, two names have been applied to populations from this region, Simia straminea Humboldt, 1812, and Alouatta macconnelli Elliot, 1910. Allen (1911, 1916) unquestioningly accepted the validity of A. macconnelli from the Guyana coast, but most subsequent taxonomic revisions (Cruz Lima, 1945; Cabrera, 1957; Husson, 1978) have synonymized it with S. straminea. Tate (1939) and Hill (1962) listed Alouatta seniculus macconnelli, but both doubted its validity. Nevertheless, recent cytogenetic and morphological studies, without due consideration of the taxonomic history of the two scientific names, have led to the resurrection of A. macconnelli, as distinct from S. straminea. The use of the name Alouatta macconnelli has evidently arisen from a cursory reading of Hill (1962) or an uncritical interpretation of his provisional subspecific distribution map or both. There are also contradictory interpretations of a reciprocal translocation as indicating that Simia straminea and Alouatta macconnelli are separate species (Bonvicino et al., 1995) or the same subspecies (Sampaio et al., 1996). Doubts about the type locality of Simia straminea Humboldt, 1812, as given by Hill (1962), led us to research its original description and to conclude that Simia straminea is a synonym of Alouatta caraya and therefore unavailable for Alouatta seniculus. Before A. macconnelli is accepted as the next available name for the Guianan red howlers, however, we advocate a thorough review of Guianan, Venezuelan, and Colombian red howler subspeciation, with due consideration for the taxonomic status of Mycetes auratus and Mycetes laniger Gray, 1845. We note that Alouatta guariba (Humboldt, 1812) is a senior synonym of Alouatta fusca (Saint-Hilaire, 1812).
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  • 28
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    Plant systematics and evolution 209 (1998), S. 1-31 
    ISSN: 1615-6110
    Keywords: Fabaceae ; Podalyrieae ; Liparieae ; Morphology ; chromosome numbers ; chemical compounds ; taxonomy ; generic relationships
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Taxonomic relationships amongst the genera of the southern African tribesPodalyrieae andLiparieae are discussed. Data gained from morphological, cytological and chemical investigations are analyzed cladistically to determine relationships. The genusCadia (tribeSophoreae) is included in the investigation to establish whether it should be transferred to thePodalyrieae. The results clearly indicate that thePodalyrieae andLiparieae are monophyletic and that they should be united, but thatHypocalyptus andCadia should be excluded. Within the monophyletic group, there are two distinct subclades each supported by three apomorphies. The results also show that there is a strong sister relationship betweenAmphithalea andCoelidium. In the taxonomic treatment theLiparieae are placed into synonymy under thePodalyrieae and two subtribes are recognized. A key to the genera in the tribe is given, followed by a synopsis of the genera.
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  • 29
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    Plant systematics and evolution 211 (1998), S. 43-56 
    ISSN: 1615-6110
    Keywords: Ranunculaceae ; Adonis ; Morphology ; growth pattern ; taxonomy ; ecology ; climatic requirements ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Adonis sect.Consiligo is split into three subsections based on morphology, growth pattern, pollen and fruit morphology. For all species distribution maps are provided and discussed with regard to their ecology and climatic requirements. These data reveal additional characters that support the classification of the taxa. Most species of the section grow in continental Eurasiatic steppes and mountain steppe habitats. Only the species of subsect.Amurenses grow in Far Eastern temperate forests and prefer a more oceanic climate. The taxonomic position of these strongly divergent species is supported by the distributional and ecological data. The evolution ofAdonis sect.Consiligo occurred under strong climatic control. During evolution the taxa could only slightly widen their ecological amplitude and, thus, ecology remained rather stable except that of the subsect.Amurenses. Ecological and distributional data can be used to validate taxonomy and phylogeny and reveal additional informations.
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  • 30
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    Plant systematics and evolution 210 (1998), S. 25-39 
    ISSN: 1615-6110
    Keywords: Cyanophyta ; Cyanobacteria ; Cyanothece ; Synechococcus ; Cyanobium ; Ultrastructure ; nucleoids ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The fine structure of the type species of the genusCyanothece Komárek 1976,C. aeruginosa, is described and compared with the main cytological characteristics of morphologically related members of the generaCyanobium, Cyanobacterium andSynechococcus. Several morphological features, such as cell walls with thick outer layers containing a special type of vesicles, position of thylakoids, “keritomy” (net-like appearance of protoplast caused by arrangement of thylakoids, net-like nucleoids and/or by tendency to form intrathylakoidal spaces) and a special structure of mucilaginous envelopes were found to be characteristic of this genus, supporting its separate position among coccal cyanoprokaryotes (cyanobacteria, cyanophytes). The taxonomic significance of ultrastructural features in all mentioned genera is discussed.
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  • 31
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    Plant systematics and evolution 213 (1998), S. 121-151 
    ISSN: 1615-6110
    Keywords: Boraginaceae ; Boragineae ; Palynology ; phenetics ; stereostructure ; systematics ; taxonomy ; ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The results of a comparative survey on pollen morphology in theBoragineae by means of light, scanning and transmission electron microscopy are presented and discussed in relation to the taxonomy of the tribe. Multivariate analyses lead to the recognition of 15 pollen morphotypes for which a discriminant key is proposed. The discriminatory characters concern mainly the stereostructural and ultrastructural features of the grains, such as tectum sculpture and aperture morphology, while the number of apertures appears variable within genera and even single species. Seven out of the 12 investigated genera, as currently circumscribed, are matched by palynological data:Anchusella, Borago, Brunnera, Elizaldia, Lithodora, Symphytum andTrachystemon. On account of aperture shape,Lithodora is however closer to theLithospermeae than to theBoragineae. Other genera, and in particularNonea, show a wide variation in tectum ornamentation, shape of grains and number of apertures. Palynological data do not support a broad concept of the genusAnchusa, and point to the autonomy of the ‘satellite’ generaHormuzakia, Gastrocotyle, Phyllocara, Pentaglottis andCynoglottis.
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  • 32
    ISSN: 1615-6102
    Keywords: Cucumis sativus ; Ethylene ; Ferric-reducing capacity ; Iron deficiency ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Dicotyledonous plants respond to Fe deficiency by enhancing the capacity of their roots to reduce Fe(III) to Fe(II). It has been suggested that there are two different ferric redox systems in the roots: the standard reductase, active with ferricyanide and not inducible by Fe deficiency, and the turbo reductase, active with both ferricyanide and ferric chelates and inducible by Fe deficiency. We have used different experimental approaches to test whether or not the Fe(III)-reducing capacity of cucumber (Cucumis sativus L. cv. Ashley) roots can be explained by considering the standard and the turbo reductase as the same enzyme. For this, we used both Fe-sufficient and Fe-deficient plants, which were treated with ethylene inhibitors (cobalt or silver thiosulfate; found to inhibit the turbo reductase in a previous work), a protein synthesis inhibitor (cycloheximide), or an mRNA polyadenylation inhibitor (cordycepin). At different times after application of these inhibitors, reduction of both ferricyanide and Fe(III)-EDTA were determined. In addition, we studied the effects of pH and temperature on the reduction of ferricyanide and Fe(III)-EDTA by both Fe-sufficient and Fe-deficient plants. Results suggest that there are, at least, two different ferric redox systems in the roots. Enhancement of Fe(III)-reducing capacity (turbo reductase) by Fe-deficient plants probably requires the de novo synthesis of a (or several) protein(s), which has a high turnover rate and whose expression is presumably regulated by ethylene.
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  • 33
    ISSN: 1573-5036
    Keywords: AP PCR ; Erwinia ; Klebsiella ; nitrogen-fixing bacteria ; phytopathogenicity ; ribosomal DNA ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Enterobacteria isolates from potato tubers were able to fix nitrogen, to protect plants against phytopathogens and to produce phytohormones thus increasing the plant yield. These isolates were previously phenotypically identified as Erwinia carotovora; however, they differed from typical E. carotovora in a number of biological characteristics and were found to be nonphytopathogenic (avirulent) due to the lack of pectate lyase activity. A data matrix, containing 31 strains and 105 biological characteristics was used for computer cluster analysis. The avirulent strains formed a separate cluster more closely related to Klebsiella spp. strains (with a 0.67 level of similarity) than to typical phytopathogenic bacteria of the E. carotovora group (with a 0.48 level of similarity). A phylogenetic analysis based on restriction polymorphisms of an amplified ribosomal DNA spacer region revealed that the avirulent strains studied here were different from all Erwinia, Klebsiella and other enterobacteria species strains. The AP PCR/hybridization technique showed cross homology of amplified DNA of these avirulent strains and a lack of such homology with the DNA from strains of other species. Numerical taxonomy data, rDNA analysis and AP PCR/hybridization assays confirmed that these avirulent bacteria may be regarded as an independent group of enterobacteria.
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  • 34
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    Euphytica 100 (1998), S. 189-196 
    ISSN: 1573-5060
    Keywords: cytoplasmic effects ; inheritance ; quality ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The inheritances of thousand kernel weight (TKW), protein percentage, protein quality and grain hardness were studied through an 11 x 11 complete diallel set of bread wheat genotypes consisting of four alloplasmic lines of Selkirk, two alloplasmic lines of Siete Cerros 66, and five commercial cultivars. Genetic components accounted for 93%, 90%, 78%, and 92% of total variation for TKW, protein percentage, protein quality, and grain hardness, respectively. General combining ability (GCA) effects were dominant for TKW (48% GCA, 38% SCA [specific combining ability], and 7% reciprocal effects [RE]), protein percentage (70% GCA, 10% SCA, and 10% RE), and grain hardness (59% GCA, 29% SCA, and 4% RE). However, SCA effects dominated for protein quality (30% GCA, 43% SCA, and 5% RE). Broad- and narrow-sense heritabilities were estimated at 0.95 and 0.65 for TKW, 0.94 and 0.82 for protein percentage, 0.83 and 0.47 for protein quality, and 0.95 and 0.74 for grain hardness. Reciprocal effects were highly significant for all quality traits, but less effective than additive and non-additive gene effects. Aegilops cylindrica, Ae. ventricosa, and Triticum turgidum cytoplasms showed positive effects on TKW in some crosses. Ae. cylindrica, Ae. variabilis, and Ae. uniaristata cytoplasms seemed to have potential for improving protein percentage. T. aestivum cytoplasms were superior to alien cytoplasms for protein quality. Bolal 2973, Kiraç 66 and Bezostaja 1 cytoplasms increased protein quality in some crosses. Ae. cylindrica, Ae. variabilis, Ae. ventricosa and Ae. uniaristata cytoplasms had significant effects on grain hardness. The cytoplasmic variation in B type T. aestivum cytoplasm was found to be significant for all traits.
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  • 35
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; combining ability ; heterosis ; genetic distance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract In wheat, the possibility of introducing F1 seed into practical agriculture has been greatly enhanced by the discovery of effective chemical hybridising agents (CHAs). Although some technical and economic problems concerning the use of CHAs for large-scale production of F1 seed remain to be solved, a first group of F1 hybrids has been submitted for registration in several European countries i.e., France, England and Italy. Combining ability for grain yield and several agronomic and quality traits was studied in an eight-parent diallel cross. Highly significant combining ability effects were observed for all the traits while specific combining ability effects were statistically significant for grain yield, plant height, heading time and Chopin alveograph parameter P. The level of genetic diversity between parents as estimated using molecular markers is considered a tool for predicting the hybrid performance and heterosis of crosses. To explore this possibility, RFLP and RAPD markers were used to predict the performance of hybrids obtained from diallel and top crosses. The performance of the hybrids was determined in replicated plot trials sown at normal seed density in several locations. Coefficient of parentage (rp), based on pedigree information for all the pairwise combinations of the parents ranged from 0.01 to 0.34. The parents were assayed for random amplified polymorphic DNA (RAPD) with 87 primers which generated 304 polymorphic bands. Genetic similarity between parents, estimated on the basis of common bands using the Jaccard's similarity coefficient (J), ranged from 0.25 to 0.57. Correlation between parental diversity and hybrid performance was generally weak. A positive trend is observed in the yield potential of the hybrids produced in Italy in the last 10 years. In fact among the first set of hybrids produced by random crossing of the available cultivars, none produced 10% more than the checks whereas the last generation of hybrids includes combinations yielding 15% more than the best standards. Our results clearly indicate the need to develop specific strategies in order to identify and/or to select parental lines with a high level of general combining ability (GCA) and specific combining ability (SCA). The information regarding the genetic diversity of the parental lines do not appear helpful for predicting F1 performance.
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  • 36
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    Euphytica 101 (1998), S. 9-16 
    ISSN: 1573-5060
    Keywords: heading time ; Triticum aestivum ; vernalisation response ; Vrn – genotypes ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Substitution lines with reciprocal substitutions of chromosomes containing recessive alleles of the homoeologous group 5 chromosomeVrn genes between varieties of winter wheat with high vernalisation requirement (‘Mironovskaya 808’) and low vernalisation requirements (‘Bezostaya 1’) have been created. On this basis the genetic determination of vernalisation requirement was established. Substitution lines Mironovskaya 808 (Bezostaya 1 5A), Mironovskaya 808 (Bezostaya 1 5B), Mironovskaya 808 (Bezostaya 1 5D) and reciprocal substitution lines Bezostaya 1 (Mironovskaya 808 5A), Bezostaya 1 (Mironovskaya 808 5B) and Bezostaya 1 (Mironovskaya 808 5D) were grown under different durations of vernalisation (3, 4, 5, 6, 7 and 8 weeks) and their response was evaluated. Photoperiodic sensitivity of the original parental genotypes was also determined. Reciprocal substitution lines of the same chromosome that carries the same vrn allele responded differently to vernalisation deficit. Differences have been shown between all group 5 reciprocal substitutions. Lines carrying chromosomes 5A and 5D of Mironovskaya 808 had a high vernalisation requirement whereas lines carrying chromosome 5B of Bezostaya 1 (vrn2B) had a low vernalisation requirement. The reciprocal lines had a reverse requirement. This explains the different vernalisation requirements of the original varieties: Mironovskaya 808 with a high vernalisation requirement carries two alleles (vrn1M and vrn3M) in its genotype that increase the vernalisation requirement, whereas Bezostaya 1 with a lower requirement for vernalisation contains only one such allele (vrn2B). By combination of the alleles in the lines with the substitution of chromosome 5B carrying vrn2 allele that in both original genotypes work inversely to the other alleles, transgressive genotypes have been formed: genotype vrn1M vrn2B vrn3M determines a higher vernalisation requirement than original variety Mironovskaya 808, and genotype vrn1B vrn2M vrn3B determines a lower vernalisation requirement than the original Bezostaya 1. An incomplete vernalisation requirement prolonged the time to heading, with exponential dependence on the vernalisation deficit, or prevented heading altogether. The original varieties further differed in photoperiodic sensitivity (Mironovskaya 808 sensitive, Bezostaya 1 less sensitive) that also influenced the background of substitution lines. The impact of the background on the heading time showed itself by about one week difference between Mironovskaya 808 and Bezostaya 1 grown under 8 weeks vernalisation and normal photoperiod. The difference between the lines with Mironovskaya 808 background and the lines with Bezostaya 1 background was approximately the same and was not significantly changed in different vernalisation variants of the lines. This difference may be caused by different photoperiodic sensitivity of the original varieties, but also by other genes, such as genes of earliness per se.
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  • 37
    ISSN: 1573-5060
    Keywords: bread wheat ; Triticum aestivum ; tolerance to deoxynivalenol ; somaclonal variant ; in vitro selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This study was conducted to develop an efficient in vitro selection system for scab resistance by using in vitro screening for tolerance to deoxynivalenol (DON). Immature embryos of two wheat varieties, a scab-resistant variety Sumai 3 and a susceptible variety Mianyang 11, and their reciprocal F1 hybrids were cultured on MS medium supplemented with 2,4-D 2 mg/l and 0.6 × 10-4 M DON for callus induction. The responses of callus induction and plant regeneration to 0.6 × 10-4 M DON differed significantly between resistant and susceptible varieties, according to observed scab resistance levels at the plant level in the field. The percentage of callus formation of resistant variety Sumai 3 on induction medium containing DON was higher than that of susceptible variety Mianyang 11. Regeneration of DON-tolerant calli on DON-containing differentiation medium differed significantly between Sumai 3 and Mianyang 11. Averaged across the DON-tolerant calli of two varieties and their reciprocals, regeneration of DON-tolerant calli was decreased 3-fold on DON-containing medium. By an inoculation test with conidiospores of Fusarium graminearum Schw, we obtained several resistant lines from progenies of regenerated plants from DON-tolerant calli. These somaclonal lines had lower disease scoring (reaction index, infected spikelets and disease incidence), shorter plants and better yield components than Sumai 3, a famous Chinese resistant variety.
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  • 38
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; Secale cereale ; T1BL.1RS ; chromosome substitution and translocation ; yield components
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The T1BL.1RS wheat (Triticum aestivum L.) - rye (Secale cereale L.) translocations have been of particular interest and are widely used in bread wheat breeding programs. The objective of this study was to determine the effect of the T1BL.1RS chromosome on grain yield and its components using 20 near-isolines of spring bread wheat cultivar ‘Seri M82’ (10 homozygous for chromosome 1B substitution and 10 homozygous for T1BL.1RS). The test lines have been produced by substituting the 1B chromosome in Seri M82 (T1BL.1RS, T1BL.1RS) through backrossing. Two field experiments were evaluated under optimum (five irrigations) and reduced (one irrigation) moisture conditions for two consecutive production cycles at the Mexican National Agricultural Research Institute, Ciudad Obregon, Sonora, Mexico. The presence of T1BL.1RS had a significant effect on grain yield, harvest index, grains/m2, grains/spike, 1000-grain weight, test weight, flowering date and physiological maturity in both moisture conditions. The agronomic advantage of the 1B substitution lines on above-ground biomass yield at maturity, spikes/m2and grain-filling duration was expressed only under the optimum moisture condition. The presence of T1BL.1RS increased grain yield 1.6% and 11.3% for optimum and reduced moisture conditions, respectively. These results encourage further use of T1BL.1RS wheats in improving agronomic traits, especially for reduced irrigation or rainfed environments.
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  • 39
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    Euphytica 104 (1998), S. 39-47 
    ISSN: 1573-5060
    Keywords: drought resistance ; diallel graph ; gene action ; excised-leaf water loss ; relative water content ; bread wheat ; osmotic adjustment ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Little information is available on the genetics of excised leaf water loss and relative water content in wheat. An experiment conducted on the F1 generation from a half-diallel set of crosses involving two drought tolerant, two moderately tolerant and two sensitive varieties was initiated to investigate the inheritance of excised-leaf water loss and relative water content. This experiment was conducted under glass-house and field conditions at tillering and anthesis stages of plant development. Additive gene action, in general, played a major role in determining the inheritance of these traits. General combining ability (GCA) was the main source of genetic variation among crosses, while specific combining ability (SCA) was negligible. Strong phenotypic correlations existed between per se performance and GCA effects in the majority of cases. Heterosis was unimportant. Genotype-environmental interactions and/or differential gene expression appeared to account for different results found between environments and growth stages, respectively. Selection for relative water content appeared to be more effective at anthesis, while for excised-leaf water loss at both stages of plant growth. In addition to drought resistance, wide differences for morphological characters and relative positions of parental arrays revealed the possibility of obtaining desirable segregants for drought stress conditions from the cross Kharchia 65 × WH 147.
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  • 40
    ISSN: 1573-5036
    Keywords: biomarker ; cadmium ; copper ; heavy metal ; PC ; PC-SH ; phytochelatin ; stress ; toxicity ; Triticum aestivum ; Zea mays
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Heavy metal contaminated soils often show increased levels of more than one metal, e.g. copper (Cu), cadmium (Cd), zinc (Zn), lead (Pb) or nickel (Ni). In case such soils are used for crop production, prediction of yield reduction or quality decline due to heavy metals in the soil is inadequate when based only on chemical soil analysis. The use of biomarkers such as phytochelatins (PC), non-protein thiols specifically induced in plants upon exposure to heavy metals, may be an additional tool or diagnostic criterion in heavy metal research and in practice. In the present work, Cu and Cd uptake and induction of PC synthesis are studied with hydroponically grown maize and wheat plants exposed to mixtures of the two metals. We observed a close positive relationship between the concentrations of Cd and PC in the plant shoot material. A decreased shoot concentration of Cd after addition of Cu, due to metal competition at common root absorption sites, coincided with lower shoot PC levels. Also differences in metal uptake and xylary metal transport among the two plant species were reflected in corresponding differences in PC concentration. The observed direct relationship between shoot PC concentration and the degree of metal-induced growth inhibition makes the use of PC promising for the purpose tested for.
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  • 41
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; wheat ; Puccinia recondita ; leaf rust ; breeding ; resistance ; yield ; yield components
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The most important breeding objectives in crop improvement are improving grain yield, grain quality, and resistances to various biotic and abiotic stresses. The objectives of our study were to compare two crossing and four selection schemes for grain yield, yield traits, and slow rusting resistance to leaf rust (Puccinia recondita) based on additive genes in wheat (Triticum aestivum), and to identify the most efficient crossing and selection methodologies in terms of genetic gains and cost efficiency. Segregating populations were derived from 18 simple crosses and the same number of top (three-way) crosses. Half of the crosses were derived from Yecora 70 and the other half from Veery #10 as the common leaf rust susceptible parents. The four selection schemes were: pedigree, modified bulk (F2 and F1-top as pedigree, selected lines in F3, F4, F2-top, F3-top as bulk; and pedigree in F5 and F4-top populations), selected bulk (selected plants in F2, F3, F4, F1-top, F2-top and F3-top as bulk; and pedigree in F5 and F4-top populations), and nonselected bulk (bulk in F2, F3, F4, F1-top, F2-top and F3-top; and pedigree in F5 and F4-top populations). A total of 320 progeny lines, parents and checks were tested for grain yield, other agronomic traits and leaf rust resistance during the 1992/93 and 1993/94 seasons in Ciudad Obregon (Sonora State, Mexico) which represents a typical high yielding irrigated site. The influence of the type of cross and the selection scheme on the mean grain yield and other traits of the progenies was minimal. The selection of parents was the most important feature in imparting yield potential and other favourable agronomic traits. Moreover, the highest yielding lines were distributed equally. Progeny lines derived from Veery #10 crosses had significantly higher mean grain yield compared to those derived from the Yecora 70 crosses. Furthermore, a large proportion of the highest yielding lines also originated from Veery #10 crosses. Mean leaf rust severity of the top cross progenies was lower than that of the simple cross progenies possibly because two parents contributed resistance to top cross progenies. Mean leaf rust severity of the nonselected bulk derivatives was twice that of lines derived from the other three schemes. Selected bulk appears to be the most attractive selection scheme in terms of genetic gains and cost efficiency.
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  • 42
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    Euphytica 100 (1998), S. 253-259 
    ISSN: 1573-5060
    Keywords: hexaploid wheat ; pearl millett ; Pennisetum glaucum ; polyhaploid ; pollen storage ; Triticum aestivum ; wide crosses
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract The effects of drying and freezing on viability of pearl millet pollen were examined with the aim of using stored pollen in polyhaploid production of hexaploid wheat. Freshly collected pollen of pearl millet line NEC 7006 with 55% water content, germinated at a frequency of 80%. Pollen that was dried for two hours to 6% water content showed 50% germination frequency and maintained similar frequencies after the freezing process. In crosses of hexaploid wheat variety Norin 61 with fresh pearl millet pollen, embryos were obtained at a frequency of 27.6%. In crosses with pollen stored at -196 °C, -80 °C and -20 °C for one month, embryo formation frequencies ranged from 27.5 to 17.4%. After five and twelve months of storage, the frequencies ranged from 29.7 to 14.6% at storage temperatures of -196 °C and -80 °C, and from 8.0 to 3.2% at -20 °C, indicating significant differences among storage temperatures. However, no significant frequency difference was found among pollen water contents at the time of collection. All plants regenerated from crosses with pearl millet pollen stored for five months were wheat polyhaploids. These results suggest that stored pearl millet pollen is an efficient medium for producing polyhaploids in hexaploid wheat.
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  • 43
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    Euphytica 100 (1998), S. 305-311 
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; winter wheat ; genetic diversity ; ancestors ; genetic profile
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract With the aid of GRIS, the wheat genetic resources database, the retrospective analysis of winter wheat breeding programs in the ex-USSR during its existence, and modern Russia, was conducted. The dynamics of genetic diversity of released cultivars was studied. A significant tendency to reduce the use of local materials was discovered, although a stable set of original ancestors has prevailed over the last 40 years. The modern cultivar genes pool has increased as a result of the utilisation of North American semidwarf varieties. Breeding programs at different breeding centres are distinguished by varying levels of genetic diversity. The need to discover new sources of disease resistance and environmental adaptation are problems that remain.
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  • 44
    ISSN: 1573-5060
    Keywords: chloroplast DNA ; eggplant ; PCR ; RFLP ; Solanum ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract RFLP analysis of a PCR amplified 3.2-kbp region of cpDNA bounded by the conserved sequences in rbc L and ORF 106 was performed in eggplant (Solanum melongena), its related Solanum species, S. incanum, S. virginianum (= S. surattense), S. torvum, S. aethiopicum (= S. gilo), S. aethiopicum (= S. integrifolium), S. violaceum (= S. indicum), S. violaceum (= S. sanitwongsei) and S. mammosum and the reciprocal hybrids between S. aethiopicum (= S. integrifolium) and S. melongena 'Uttara'. The target region of cpDNA was amplified correctly by PCR. The amplified products were digested with each of 10 restriction enzymes (Alu I, Ase I, BamH I, Hinf I, Msp I, Rsa I, ScrF I, Sty I, Taq I and Xba I). Variations of restriction patterns among the species were recognized after digesting the amplified products with each of the seven restriction enzymes, Taq I, Alu I, Rsa I, Sty I, Ase I, Hinf I and Xba I. The restriction patterns divided the examined nine species into the following five clusters, 1) S. melongena and S. incanum, 2) S. virginianum (= S. surattense), 3) S. torvum, 4) S. aethiopicum (= S. gilo), S. aethiopicum (= S. integrifolium), S. violaceum (= S. indicum) and S. violaceum (= S. sanitwongsei) and 5) S. mammosum. The restriction pattern with Alu I in each of the reciprocal hybrids between S. melongena 'Uttara' and S. aethiopicum (= S. integrifolium) was identical with that of seed parent. The present study demonstrated the availability of the PCR-RFLP analysis of cpDNA for assessing taxonomic relationships and identifying cytoplasmic parentage of interspecific hybrids in eggplant and related Solanum species.
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  • 45
    ISSN: 1573-5060
    Keywords: in situ hybridization ; Psathyrostachys juncea ; chromosome translocation ; translocation addition ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Using the genomic in situ hybridization (GISH) technique, one translocation line, seven translocation-addition lines, five translocation plus translocation addition lines and two ditelosomic addition lines were identified in backcross progenies of Triticum aestivum L. -Psathyrostachys juncea (Fisch.) Nevski intergeneric hybrids. No complete P. juncea chromosomes were detected in the 25 lines studied. The results suggest that intact P. juncea chromosomes may be difficult to isolate in a wheat background.
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  • 46
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    Hydrobiologia 287-388 (1998), S. 35-37 
    ISSN: 1573-5117
    Keywords: Rotifera ; Keratella trapezoida n. sp. ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A new species of planktonic rotifer, Keratella trapezoida n. sp. is described from the Yangtze River, P.R. China. The new morphospecies is characterized by its four enclosed dorsal median facets, nearly trapezoid shape of the first median facet on dorsal plate, and the caudal median facet with parallel margins and being open posteriorly.
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  • 47
    ISSN: 1573-5109
    Keywords: multivariate analysis ; Oryza ; O. glumaepatula ; O. nivara ; O. rufipogon ; taxonomy ; wild rices
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract This study was carried out to clarify the taxonomic status of the New World diploid wild rice species, Oryza glumaepatula. The morphological variation of 26 diploid rice accessions in the International Rice Genebank at IRRI from South America and Cuba was compared with that of O. rufipogon and O. nivara from Asia. The 28 morphological characters included 16 spikelet and grain, eight leaf and culm, and four panicle characters, and were analyzed using principal component analysis (PCA) and hierarchical agglomerative cluster analysis. The first two principal components accounted for 53.6% of the total variation. The first component was characterized by leaf and sterile lemma characters, 5-panicle dry weight, and grain length, and the second by anther length and its ratio to grain length, grain width, thickness, and 20-hulled grain weight. The characters effectively differentiated the wild rices from Surinam, French Guiana, and the lower Amazon River basin in Brazil, which showed high negative scores along both axes, from other accessions from Venezuela, Colombia, and Cuba, and the Asian species. Most of the accessions from South America are quite distinct from O. rufipogon, with which they have often been grouped as a single species in some taxonomic treatments. Groupings obtained from cluster analysis corresponded closely with the results from PCA. This study supports a distinct taxonomic status of a group of diploid wild rices from South America as O. glumaepatula.
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  • 48
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    Hydrobiologia 287-388 (1998), S. 9-14 
    ISSN: 1573-5117
    Keywords: taxonomy ; evaluation ; Rotifera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Results are presented of a historical analysis of taxonomic research on Rotifera, as reflected by the case of α-taxonomy of Lecanidae and Dicranophoridae. The number of available names established, as well as the fraction presently considered valid are counted per decade. Two peak periods in taxonomic research are revealed, viz. a minor one in the last decades of the 19th century, and a major one in the 1920s–1930s. Especially work published during the second period contains a high proportion of names that are currently considered valid. The second half of the 20th century witnessed a decrease in quantity, but also in quality of taxonomic research. The basic cause for this is probably the typological approach to a group exhibiting high intraspecific morphological variability, but also poor taxonomic education, as reflected by a high incidence of insufficient descriptions, and poor knowledge of the rules governing zoological nomenclature, are of incisive importance.
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  • 49
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    Hydrobiologia 287-388 (1998), S. 109-115 
    ISSN: 1573-5117
    Keywords: behavior ; mating ; evolution ; taxonomy
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    Topics: Biology
    Notes: Abstract The recent isolation of a mate recognition pheromone in the marine rotifer Brachionus plicatilis Müller has shed new light on the mate recognition system of rotifers. One result is improved understanding of the importance of mating behavior as a highly efficient process used by rotifers to choose conspecifics. There are many differences in the main characteristics of mating behavior in members of five different families of rotifers. The present work describes the use of these characteristics to assess species boundaries, especially where boundaries between two or more species are unclear. The method proposed here can assess quantitatively the response of males of one species to females of a questionable taxon by measuring the percentage of matings initiated and the number of completed copulations. The data generated can then be used together with molecular, morphological, and other data to determine the species boundaries. This approach can help distinguish between morphological differences resulting from evolutionary divergence of species and morphological differences induced by environmental or ecological factors.
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  • 50
    ISSN: 1573-5117
    Keywords: Ilyocryptus ; Anomopoda ; new species ; taxonomy ; Australia ; Queensland
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract All representatives of the genus Ilyocryptus with short lateral setae at the postanal margin of the postabdomen may be separated into the compact species group I. brevidentatus s. lat. A new species of Ilyocryptus from this group is described from the Lake Numalla, Queensland, Australia. I. smirnovi n. sp. shows clear morphological differences in the armature of the valve, postabdomen, and several other structures, from all other species of the group. Differences between members of the above-mentioned species group are analogous with those between European species, listed by Štifter (1991).
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  • 51
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    Hydrobiologia 369-370 (1998), S. 297-313 
    ISSN: 1573-5117
    Keywords: planktonic phytoflagellates ; taxonomy ; seasonality ; hypertrophic brackish water ; tropical coastal lagoon ; Brazil
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The phototrophic flagellate flora of the Barra Lagoon (22°57′ S and 42°47′ W), Rio de Janeiro State, southeastern Brazil, is described. The lagoon is a shallow, oligohaline, hypertrophic ecosystem in which Cyanophyceae, Chlorophyceae and phytoflagellates are dominant. Between October 1990 and August 1993, weekly integrated samples were collected from a bay, using a plexiglass tube, 8 cm diameter and 1m length. All identifications, descriptions and measurements were carried out on live or in Lugol-fixed material. A total of 30 taxa, belonging to Euglenophyceae (5), Cryptophyceae (7), Raphidophyceae (1), Dinophyceae (4), Chrysophyceae (3), Prymnesiophyceae (2), Prasinophyceae (7) and Chlorophyceae (1) were identified. All the phytoflagellate species identified have been recorded previously from temperate sites. Nine of them were registered in a tropical water for the first time, suggesting that they have much broader distributions. Gymnodinium sanguineum, Pavlova lutheri and Pyramimonas grossii were the most frequently observed phytoflagellates in this study. The seasonal occurrence and some environmental requirements of selected phytoflagellates species are discussed.
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  • 52
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    Hydrobiologia 379 (1998), S. 23-32 
    ISSN: 1573-5117
    Keywords: Arthropsyllus australis sp. n. ; Ancorabolidae ; taxonomy ; South America ; Chile
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A new species of the family Ancorabolidae Sars, 1909 (Copepoda, Harpacticoida), Arthropsyllus australis sp. n. was collected 1994 during 'Magellan Campaign' of RV 'Victor Hensen' and is described in the present paper. Its inclusion in the genus Arthropsyllus Sars, 1909 is justified by the diagnostic characters of the genus: lack of any dorsal or dorsolateral well-developed processes, body slightly depressed dorsoventrally, body somites with lateral epimeres except cephalothorax and last two abdominal somites. A. australis sp. n. differs from Arthropsyllus serratus Sars, 1909 in the shape of the rostrum, in having moderately-sized sensilla-bearing dorsal and dorsolateral projections on cephalothorax and body somites, and in several differences on mandible, maxillule, maxilla and swimming legs.
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  • 53
    ISSN: 1573-5117
    Keywords: Potamidae ; Carpomon pomulum ; taxonomy ; new genus ; new species ; Philippines
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A new genus and new species of terrestrial potamid crab ( Carpomon pomulum) is described from the island of Palawan in the Philippines. This new genus can easily be distinguished from all other Philippine potamids in its smooth and inflated carapace, no trace of an epibranchial tooth, extremely low postorbital cristae, and stout and twisted male first gonopod.
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  • 54
    ISSN: 1573-5117
    Keywords: Marine nematodes ; Diplolaimella thailandica ; Thalassomonhystera siamensis ; Theristus (Penzancia) longisetifer ; new species ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Three new species belonging to the genera Diplolaimella and Thalassomonhystera of the family Monhysteridae and Theristus of the family Xyalidae are described from shrimp culture ponds near mangrove zones of Khung Kraben Bay, Thailand. Diplolaimella thailandica sp. n. resembles D. dievengatensis but differs from it by features of the ratios b and V, and size of the spicules and tail of the male. Thalassomonhystera siamensis sp. n. resembles T. diplops but differs from it in features of the amphids, ocelli, cloaca and gubernaculum. Theristus (Penzancia) longisetifer sp. n. resembles T. flevensis, T. ambronensis, T. macroflevensis, and T. pratti but differs from them in features of the cervical setae, ventral supplement of the male and others.
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  • 55
    ISSN: 1573-5117
    Keywords: Calanoid copepod ; taxonomy ; Sinodiaptomus valkanovi ; S. sarsi ; limnoplankton ; Japan
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The limnoplanktonic calanoid copepod Sinodiaptomus valkanovi (Diaptomidae: Diaptominae) is redescribed in detail from Japan, where it commonly occurs in ponds and reservoirs especially around the Seto Inland Sea. The species has been referred to as a subspecies of S. sarsi outside Japan due to lack of subsequent morphological comparisons. Sinodiaptomus valkanovi is easily distinguishable from S. sarsi by having longer antennules in both sexes, a short projection on the right antennule segment 15 of the male, and a thumb-like posterodistal basal projection of the male left leg 5. These characters do not show significant variation, indicating differentiation at the species level. S. valkanovi is restricted to Japan, in contrast to the wide-range of it S. sarsi in Asian waters. The Bulgarian population, from which the species was originally described, and the North American population, which was formerly recorded as S. sarsi, are considered to have been introduced from Japan with aquatic plants such as water lilies.
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  • 56
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    Hydrobiologia 387-388 (1998), S. 47-54 
    ISSN: 1573-5117
    Keywords: Mexico ; Rotifera ; new record ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A survey of rotifers from a small pond (less than 2 ha in area and 3 m deep), located at Kilometer 28 in the federal highway Ixtlahuaca-Jilotepec (19° 49′ 13″ N, 99° 42′ 22″ W) at an altitude of 2503 m above sea level, resulted in a total of 78 species. From these, 20 are new records for Mexico. This study confirms the presence of some of the rotifer species listed only in earlier studies. Comments on some species are made from a zoogeographical point of view.
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  • 57
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    Hydrobiologia 387-388 (1998), S. 109-115 
    ISSN: 1573-5117
    Keywords: behavior ; mating ; evolution ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The recent isolation of a mate recognition pheromone in the marine rotifer Brachionus plicatilis Müller has shed new light on the mate recognition system of rotifers. One result is improved understanding of the importance of mating behavior as a highly efficient process used by rotifers to choose conspecifics. There are many differences in the main characteristics of mating behavior in members of five different families of rotifers. The present work describes the use of these characteristics to assess species boundaries, especially where boundaries between two or more species are unclear. The method proposed here can assess quantitatively the response of males of one species to females of a questionable taxon by measuring the percentage of matings initiated and the number of completed copulations. The data generated can then be used together with molecular, morphological, and other data to determine the species boundaries. This approach can help distinguish between morphological differences resulting from evolutionary divergence of species and morphological differences induced by environmental or ecological factors.
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  • 58
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    Hydrobiologia 387-388 (1998), S. 9-14 
    ISSN: 1573-5117
    Keywords: taxonomy ; evaluation ; Rotifera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Results are presented of a historical analysis of taxonomic research on Rotifera, as reflected by the case of α-taxonomy of Lecanidae and Dicranophoridae. The number of available names established, as well as the fraction presently considered valid are counted per decade. Two peak periods in taxonomic research are revealed, viz. a minor one in the last decades of the 19th century, and a major one in the 1920s–1930s. Especially work published during the second period contains a high proportion of names that are currently considered valid. The second half of the 20th century witnessed a decrease in quantity, but also in quality of taxonomic research. The basic cause for this is probably the typological approach to a group exhibiting high intraspecific morphological variability, but also poor taxonomic education, as reflected by a high incidence of insufficient descriptions, and poor knowledge of the rules governing zoological nomenclature, are of incisive importance.
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  • 59
    ISSN: 1573-6857
    Keywords: genus Pellia ; liverworts ; RAPD ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The random amplified polymorphic DNA (RAPD) technique was used to study the genomic relationships of three liverworts from the genus Pellia: P. epiphylla, P. borealis and P. neesiana. Altogether 150 characters (150 DNA fragments obtained using PCR) were scored. These characters were used to create a matrix of pairwise distances between all the pairs of taxa. Both distance (UPGMA, Fitch–Margoliash and Neighbor–Joining) and binary character‐state (Wagner and Camin‐Sokal parsimony and compatibility) methods were applied for trees' construction. Our results strongly support distinction of the recently discovered sibling species of P. epiphylla – species N and P. epiphylla – species S, which have an allopatric distribution in Poland (N – North, S – South Poland). Moreover, our data also supports the hypothesis of a hybrid origin (alloploid) of the polyploid P. borealis from P.epiphylla−N×P.epiphylla−S. P. neesiana was excluded as a donor of either of the genomes of P. borealis.
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  • 60
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    Hydrobiologia 377 (1998), S. 205-211 
    ISSN: 1573-5117
    Keywords: Crustacea ; Branchiopoda ; Anomopoda ; Alona rusticoides n. sp. ; taxonomy ; Venezuela ; 'Cladocera'
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Seven anomnopodan species were recorded from top of Roraima and Ptari tepuis. Alona rusticoides is new species and Acroperus sp., Alonella cf. excisa and Paralona pigra seem to be different from species described from Europe.
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  • 61
    ISSN: 1572-9699
    Keywords: Myxozyma neglecta ; Myxozyme mucilagina ; Candidaceae ; mol% G + C ; DNA-DNA homology ; yeasts ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Three strains of Myxozyma mucilagina including the type strain were reexamined. Based on differences in their carbon utilization pattern, mobility of isoenzymes, mol% G + C of their DNA and extent of DNA complementary the new species Myxozyma neglecta is proposed.
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  • 62
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    Genetic resources and crop evolution 45 (1998), S. 263-269 
    ISSN: 1573-5109
    Keywords: evolution ; interspecific hybridization ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A new species of oat, Avena insularis, is described. It was collected in southern Sicily where four populations were found on uncultivated clay soil. Morphologically, it is similar to the hexaploid wild oat A. sterilis, but can be distinguished by its smaller and more condensed panicle, less V-shaped dispersal unit and oblong disarticulation scar. Hybrids between A. insularis and the hexaploid cultivated oat A. sativa were obtained only when the latter was the seed parent in crosses. Chromosome pairing of the hybrids at meiosis was irregular with univalents and multivalents, but the mean number of chiasmata per cell was close to that of A. insularis. Furthermore, the hybrids were partially self-fertile. Thus, the newly discovered species seems closer to the hexaploid oats than any other tetraploid species, and is probably the tetraploid progenitor of hexaploid oats. Hybrids between A. insularis and A. magna were sterile because of irregular chromosome pairing at meiosis.
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  • 63
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    Hydrobiologia 287-388 (1998), S. 63-77 
    ISSN: 1573-5117
    Keywords: Dicranophoridae ; Austria ; alpine water bodies ; taxonomy ; biogeography ; ecology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Rotifera of the family Dicranophoridae Harring, 1913 were recorded from mountainous altitudes of the Austrian Alps. Here, their morphology, distribution and ecology is detailed. The description of Encentrum walterkostei Jersabek is amended by observations on living animals. Of 19 species encountered, all but four are new to the alpine region, nine species are first records for the biogeographic region 'Alps'. Four species are new to science and will be published elsewhere. The majority are cosmopolites or widely distributed taxa, but also species with a more limited range, possibly endemics, seem to exist. Most species can be characterized as being more commonly found in cold environments, some of them are known to be psammobiontic or psammophilic.
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  • 64
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    Hydrobiologia 287-388 (1998), S. 47-54 
    ISSN: 1573-5117
    Keywords: Mexico ; Rotifera ; new record ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A survey of rotifers from a small pond (less than 2 ha in area and 3 m deep), located at Kilometer 28 in the federal highway Ixtlahuaca-Jilotepec (19° 49′ 13″ N, 99° 42′ 22″ W) at an altitude of 2503 m above sea level, resulted in a total of 78 species. From these, 20 are new records for Mexico. This study confirms the presence of some of the rotifer species listed only in earlier studies. Comments on some species are made from a zoogeographical point of view.
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    Hydrobiologia 287-388 (1998), S. 27-33 
    ISSN: 1573-5117
    Keywords: Rotifera ; biodiversity ; floodplain ; taxonomy ; Thailand
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A survey of 11 freshwater habitats in the floodplain of the River Nan, northern Thailand was carried out during April and September 1996. The rotifer samples were collected qualitatively from paddy fields, ponds, canals and reservoirs, using a 60 μm mesh net. One hundred and eighteen species were identified, four (Lepadella quinquecostata (Lucks), Macrochaetus danneeli Koste & Shiel, Testudinella ahlstromi Hauer and T greeni Koste) of which are new to Thailand and one (L. quinquecostata) is new to Asia. The numbers of species found in two localities are relatively high, with 86 and 73 rotifer taxa. Most of the species recorded are common, cosmopolitan or pantropical and warm-stenotherms. The occurrence of a species previously considered endemic to Australia, M. danneeli provides more evidence illustrating a relation between the rotifer faunas of southeast Asia and Australia. Comments are presented on some insufficiently known taxa in particular on the new records for Thailand.
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  • 66
    ISSN: 1573-5060
    Keywords: Triticum aestivum ; Heterodera avenae ; resistance ; breeding
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Breeding wheat for resistance to Heterodera avenae in southern Australia has been in progress for nearly 30 years and recently a number of resistant varieties have been released. Early breeding work was hampered by three factors: • a lack of appreciation of the role and extent of the problem, • inaccurate, slow screening methods, ultimately being replaced by the 'tube' test and soon by linked molecular markers, • inappropriate breeding strategies, so that varietal releases have taken place only when the breeding has been fully integrated into the main programs. The experiences in southern Australia will be relevant to many other areas in the world where H. avenae is the major pest.
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  • 67
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    Euphytica 100 (1998), S. 131-135 
    ISSN: 1573-5060
    Keywords: boron ; tolerance ; Triticum aestivum
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    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A new method for screening wheat for boron tolerance has been developed to overcome the problems of methods used in the past. Seedlings were grown for 10 days while suspended over B-rich solutions. Their root lengths were then measured as an index of tolerance. Five varieties of wheat were screened at seven concentrations of B. Results were obtained more quickly and easily than from the alternative methodologies and compared favourably. Screening of 128 doubled haploid lines from a cross between Halberd and (Wl*MMC) suggested that transgressive segregation had occurred.
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  • 68
    ISSN: 1573-5060
    Keywords: leaf rust ; Puccinia recondita f. sp. tritici ; resistance genes ; Triticum aestivum ; Triticum monococcum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Monosomic analysis was conducted to determine chromosomal locations of three new leaf rust resistance genes recently transferred to common wheat (Triticum aestivum) from T. monococcum. The resistance gene in wheat germplasm line KS92WGRC23 was transferred from T. monococcum ssp. monococcum. The resistance genes found in KS93U3 and KS96WGRC34 were transferred from T. monococcum ssp. aegilopoides. Allelism tests showed that the three resistance genes were unlinked. The three lines were crossed with each of the seven A-genome Wichita monosomic lines. The leaf rust resistance genes in KS92WGRC23, KS93U3, and KS96WGRC34 were located on chromosomes 6A, 1A, and 5A, respectively, by monosomic analysis. These results demonstrate that the three new genes derived from T. monococcum are each different. They also differ from previously reported Lr genes. This information on chromosome location and the development of mapping populations will facilitate molecular tagging of the new genes.
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  • 69
    ISSN: 1573-5060
    Keywords: Citrus reticulata ; mandarin ; germplasm analysis ; genetic similarity ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract RAPD markers were used to evaluate genetic similarity among 35 mandarin accessions, including 10 species and 7 hybrids. One octamer and twenty-two decamer primers produced 109 RAPDs, 45 of which were polymorphic. Jaccard coefficient was used to calculate genetic similarity, and UPGMA to generate the phenogram. The RAPDs obtained were sufficient to generate some accession-specific markers, and to separate these accessions by clustering them into several groups, many of them according to Tanaka's or Webber's systematic units. The genetic similarity within the mandarin group is high (GJ = 0.77), and suggests that cultivated mandarins have a narrow genetic base. The genetic similarity of mandarins to other true citrus species (Citron [C. medica L.] and Pummelo [C. grandis Osbeck]) was much lower (minimum GJ = 0.27). We propose that the mandarin group is a single species, C. reticulata Blanco, composed of several genetically different individuals and a great number of hybrids, rather than a large number of species as proposed by some taxonomic studies.
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  • 70
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    Euphytica 102 (1998), S. 219-226 
    ISSN: 1573-5060
    Keywords: antifreeze proteins ; chromosome substitution lines ; cold acclimation ; freezing tolerance ; Triticum aestivum ; winter survival
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Antifreeze proteins (AFPs) accumulate in the leaves of winter cereals during cold acclimation, where they may inhibit ice recrystallization during freezing and thawing cycles and provide nonspecific disease resistance. In this study, 21 wheat chromosome substitution lines and the parental lines Chinese Spring and Cheyenne wheat were used to determine the heritability of AFPs and the relationship between the accumulation of AFPs and winter survival. In cold-acclimated lines, antifreeze activity in leaf apoplastic extracts ranged from 1 (low) to 5 (high) with an average value of 3.2, and the accumulation of apoplastic proteins ranged from 30 μg (g FW)-1 to 115 μg (g FW)-1 with a mean value of 70 μ (g FW)-1. Examination of the individual lines revealed that Cheyenne chromosomes 5B and 5D carry major regulatory genes that increase both antifreeze activity and the accumulation of antifreeze proteins in plants grown at low temperature. Substitution lines carrying Cheyenne chromosomes 2A, 3A, 6B, and 7A exhibited lower freezing tolerance and also showed a marked decrease in the accumulation of specific AFPs during cold acclimation. Antifreeze activity and apoplastic protein content were not correlated with freezing tolerance (defined as % survival at -11 °C), but they were both significantly and positively correlated with winter field survival rates. Antifreeze activity (positively correlated) and total leaf fresh weight (negatively correlated) together accounted for about 55% of the variation in winter survival, indicating that high antifreeze activity and slow vegetative growth at low temperature are both important quantitative traits for winter survival.
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  • 71
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    Euphytica 100 (1998), S. 359-369 
    ISSN: 1573-5060
    Keywords: genetics ; intrinsic earliness ; photoperiodic response ; vernalisation requirement ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Genetic systems regulating bread wheat ontogenesis have been studied at Ukraine's Plant Breeding and Genetics Institute, for more than two decades. The influence of Vrn genes is the most obvious; dominant alleles of Vrn genes inhibit the vernalisation requirement. The Vrn genotypes of more than 1000 cultivars were determined and the peculiarities of gene geography were shown. Dominant Vrn1 or Vrn2 seemed to be replaced by Vrn3 in regions closer to the equator. In the developed sets of near-isogenic (congenic) lines, the value of different genes was characterised for certain environments (favourable – phytotron, natural – early or late drought) based on their effects. Methods of Vrn gene manipulation were elaborated, including methods for winter genotype selection from spring x spring crosses. The possibility of alien homoeologous Vrn loci introgression was shown. In the introgressed lines, the new genes were identified and found to be nonallelic to known Vrn genes in wheat. In studying congenic lines for three Ppd genes, differences were observed in duration and intensity of photoperiodic response, vernalisation requirement and effects on agronomic traits. For typical winter wheats, two loci were identified that influenced the duration of the vernalisation requirement. One system, controlling intrinsic earliness, might be responsible for the differences in reaction to light intensity, as selection of earlier genotypes is supposed to be more effective at lower light intensity.
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  • 72
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    Euphytica 99 (1998), S. 1-4 
    ISSN: 1573-5060
    Keywords: allele ; hybrid chlorosis ; Secale cereale ; rye ; Triticum aestivum ; bread wheat ; Triticum macha ; makha wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Fourteen accessions of rye when crossed to Triticum aestivum cv. C 306 (Ne1ne2ch1Ch2) yielded chlorotic F1 hybrids and six accessions involved in hybrid combination with the same tester produced normal F1 hybrid plants. Two rye accessions, namely, EC 179188 and EC 143825 when crossed to the wheat lines HD 2329 (ne1Ne2ch1Ch2) and NI 5439 (ne1ne2ch1Ch2) also produced chlorosis. The hybrids between T. macha and two rye accessions produced normal plants. Variable degrees of chlorosis were observed among different wheat × rye F1 hybrids. It is suggested that the rye accessions producing chlorosis in combination with wheat cvs. C 306, HD 2329 and NI 5439 (all Ch2-carriers) carry one of the complementary genes for chlorosis. Gene symbol Chr1 is proposed for the chlorosis gene of rye.
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  • 73
    ISSN: 1573-5060
    Keywords: genetic diversity ; Triticum aestivum ; Triticum aestivum ssp. tibetanum ; Triticum spelta ; RAPD marker
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract An endemic hexaploid wheat found in Tibet, China was taxonomically classified as a subspecies in common wheat, i.e. Triticum aestivum ssp. tibetanum. Seven accessions of the Tibetan wheat, 22 cultivars of common wheat and 17 lines of spelt wheat were used for RAPD analysis to study the genetic relationships of the Tibetan wheat with common wheat and spelt wheat, and to assess the genetic diversity (GD) among and within the taxa. RAPD polymorphism was found to be much higher within spelt wheat and the Tibetan wheat than within common wheat. The GD value between the Tibetan wheat and common wheat is lower than that between the Tibetan wheat and spelt wheat. The result of cluster analysis showed that the 46 genotypes were distinctly classified into two groups. Group 1 included all European spelt wheat lines, while group 2 includes all Chinese common wheat and the Tibetan wheat accessions. However, the Tibetan wheat was substantially differentiated from Chinese common wheat at a lower hierarchy. Our results support an earlier classification of the Tibetan wheat as a subspecies in common wheat. European spelt wheat and the Tibetan wheat showed much higher genetic diversity than Chinese common wheat, which could be used to diversify the genetic basis for common wheat breeding.
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  • 74
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    Hydrobiologia 382 (1998), S. 119-136 
    ISSN: 1573-5117
    Keywords: Copepoda ; taxonomy ; Africa ; freshwater
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Paracyclops longispina n. sp. and Paracyclops altissimus n. sp. are described. New characters derived from detailed examination of body and limb ornamentation are used to differentiate them from other Paracyclops species. Paracyclops longispina n. sp. resembles Paracyclops fimbriatus (Fischer, 1853) and Paracyclops imminutus Kiefer, 1929. It differs from the former by the presence of a well-developed spinular row near the base of the inner setae on the antennal coxobasis in both sexes, and from the latter in the structure of the seminal receptacle and the position of the mid-distal spinular row on the posterior surface of the coxa of leg 1. Paracyclops altissimus n. sp. can be distinguished from other members of genus mainly by the structure of the seminal receptacle and leg 5.
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  • 75
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    Hydrobiologia 382 (1998), S. 175-181 
    ISSN: 1573-5117
    Keywords: Keratella new species ; taxonomy ; Rotifera ; zoogeography
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    Topics: Biology
    Notes: Abstract Two new morphospecies of Keratella Bory de St. Vincent (Rotifera: Brachionidae) are described from Inner Mongolia, P.R. China. Keratella mongolianum n. sp. is related to K. cruciformis and K. eichwaldi, while Keratella zhugeae n. sp. belongs to the K. quadrata group. K. mongolianum n.sp. is known from a single locality only, but K. zhugeae n.sp. was found in several ponds and had previously been recorded from Tibet. Both taxa are restricted to the eastern Palaearctic region.
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  • 76
    ISSN: 1573-5117
    Keywords: Terricola ; planarian ; viscid gland ; reproduction ; collagen ; taxonomy
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    Topics: Biology
    Notes: Abstract Diverticula are present in the copulatory organs of many species of the Terricola. In some species these structures are copulatory bursae or resorptive vesicles. The function of diverticula present in the copulatory organs in species of Dolichoplana and Platydemus (Rhynchodemidae), in Australoplana (Geoplanidae) and in some other caenoplaninid genera has been an enigma. From histochemical and other investigations of the ventro-posterior copulatory diverticulum in Platydemus manokwari de Beauchamp, it is evident that this structure is neither a copulatory bursa, resorptive vesicle nor a uterus. It is an adhesive gland producing a viscid, collagen-like fibrous glycoprotein derived from two secretory elements present in the gland. The secretion is expelled during cocoon laying to adhere the cocoon onto the substratum. The diverticulum appears late in the development of the copulatory organs, and is present when the gonopore is patent; this has implications for the taxonomy of platydemid flatworms.
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  • 77
    ISSN: 1573-5117
    Keywords: Platyhelminthes ; Tricladida ; Dendrocoelidae ; Bdellocephala ; Baikal ; taxonomy ; phylogeny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract On the basis of newly collected material the subspecies Bdellocephala angarensis bathyalis Timoshkin & Porfirjeva, 1989 is raised to full species status, B. bathyalis Timoshkin & Porfirjeva, 1989. Specimens of this species have been collected in Lake Baikal from depths ranging between 610 and 1060 m. The species is characterized by a light, uniform brown pigmentation, absence of eyes, distinct atrial folds, and large size. It is suggested that two features may be useful in elucidating the phylogenetic relationships between dendrocoelid genera: pharynx musculature, and presence of an extra layer of circular muscle in the ventral subepidermal body musculature.
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  • 78
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    Hydrobiologia 383 (1998), S. 307-313 
    ISSN: 1573-5117
    Keywords: Turbellaria ; Tricladida ; Polycelis ; Seidlia ; taxonomy ; distribution ; Japan ; Far East
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Polycelis (Polycelis) sapporo (Ijima et Kaburaki, 1916) is a non-stenothermal species, common in Hokkaidô (including all of the adjacent islands), Northern Japan, except in the alpine region. It is also distributed in Aomori Prefecture in the northernmost part of Honshû. To the south, its range is bounded by a line from the base of the Tsugaru Peninsula, over Mt. Iwaki, Mt. Shirakami, Mt. Tashiro, Lake Towada, Mt. Hakkôda, the Natsudomari Peninsula, to the west of Mutsu and Ôhata in the Shimokita Peninsula. In Russia, the species is recorded only from the southern part of Sakhalin. Seidlia schmidti (Zabusov, 1916) is a stenothermal species found in cold-water biotops. It occurs in the North and East of Hokkaidô including the Shiretoko Peninsula and Rishiri Island. In Russia, the species is recorded from the southern part of Sakhalin, Primorskiy, Habarovsk including the base of the Chukotskiy Peninsula, and the Kamchatka Peninsula (and Bering Island).
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  • 79
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    Hydrobiologia 383 (1998), S. 51-59 
    ISSN: 1573-5117
    Keywords: muscle ; Turbellaria Acoela ; taxonomy ; body wall
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    Topics: Biology
    Notes: Abstract While turbellarians are generally assumed to have body-wall musculature consisting routinely of longitudinal, circular, and diagonal fibers, members of the Acoela examined by a fluorescence-microscopy technique specific for actin showed more complicated and distinctive arrangements of muscles, giving promise for better delimiting taxa within this taxonomically difficult order. Certain globose or tear-drop-shaped worms such as Convoluta pulchra and species of Pseudaphanostoma, Mecynostomum, and Otocelis, showed a complex pattern in which muscles longitudinal in the anterior half of the body arc diagonally across the posterior half; complex brushes of parenchymal muscles that cross at the level of the statocyst and arc postero-laterally also characterize these groups. The more elongate acoel Paratomella sp. was found to have musculature dominated by strictly longitudinal fibers and with relatively weak circular fibers and few fibers running diagonally to the body axis, yet the elongate mecynostomid Paedomecynostomum bruneum showed a crossing of antero-longitudinal fibers similar to that seen in the more globose Mecynostomum sp. A distinctive looping of muscles around the mouth is seen in P. bruneum and the Anaperidae. Such similarities and differences in pattern of musculature promise to provide easily recognizable characters for taxonomy of the Acoela at levels ranging from species to family.
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  • 80
    ISSN: 1573-5117
    Keywords: Branchiopoda ; Anomopoda ; Radopoda ; trunk limbs ; taxonomy ; morphology ; evolution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract An investigation, using optical microscopy and SEM, of the trunk limbs of the Anomopoda has revealed a large number of characters, previously underused or unused in taxonomy and comparative morphology. All these characters, which are nicely paralleled by some more conventional traits (head shield and pores, postabdomen, antennae ...), show one clear tendency across all groups studied: a state of complexity at one extreme, and a state of often incisive simplification at the other extreme, with a number of transitional stages in between. The complex character state, which itself is a simplification of the leg structure of the Ctenopoda and other, ‘large’ Branchiopoda, is here considered to represent a primitive condition. The simplified state is considered advanced. Based on this assumption, we list a number of unifying characters (mainly structural aspects of P1 and P2, but also the gnathobase of P3 and P4) for all macrothricid and chydorid-like anomopods, which we unite in the new suborder Radopoda. Non-radopod Anomopoda are not reclassified. We then derive a cascade of (mainly trunk-limb based) characters to work out a hypothesis on the evolution of the Radopoda. The ‘chydorid’ line (basically the former family Chydoridae) is classified as a superfamily (the Eurycercoidea), with three families; the ‘macrothricid’ line is capped by the superfamily Macrothricoidea, with four families. Of these seven families, four are upgraded from subfamily status, the Chydoridae are left status quo, the Macrothricidae are redefined, and the Neothricidae are a new family. The Macrothricidae are further subdivided in two subfamilies, of which the Macrothricinae appear reasonably homogeneous (monophyletic), while the non-Macrothricinae require further study. Some of these (e.g. Guernella) have almost completely lost their P5, a situation parallel to that of the P6 in the Eurycercidae, Acantholeberidae, and Ophryoxidae.
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  • 81
    ISSN: 1573-5117
    Keywords: Encentrum n. sp. ; Notholca n. sp. ; taxonomy ; marine ; Greenland ; Arctic
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The rotifer fauna from two localities at an Arctic beach at Disko Island was investigated. Five species were found; four are new to Greenland and two of these are new to science. Encentrum graingeri and Proales reinhardti were found in the psammon, and it is suggested that they inhabit the sea-ice during the winter and then shift to inhabit the psammon in the ice-free periods during summer. Encentrum porsildi n. sp. and Notholca angakkoq n. sp. are described. The descriptions are based on detailed SEM-studies of the trophi.
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  • 82
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    Hydrobiologia 387-388 (1998), S. 35-37 
    ISSN: 1573-5117
    Keywords: Rotifera ; Keratella trapezoida n. sp. ; taxonomy
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    Notes: Abstract A new species of planktonic rotifer, Keratella trapezoida n. sp. is described from the Yangtze River, P.R. China. The new morphospecies is characterized by its four enclosed dorsal median facets, nearly trapezoid shape of the first median facet on dorsal plate, and the caudal median facet with parallel margins and being open posteriorly.
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  • 83
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    Hydrobiologia 387-388 (1998), S. 63-77 
    ISSN: 1573-5117
    Keywords: Dicranophoridae ; Austria ; alpine water bodies ; taxonomy ; biogeography ; ecology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Rotifera of the family Dicranophoridae Harring, 1913 were recorded from mountainous altitudes of the Austrian Alps. Here, their morphology, distribution and ecology is detailed. The description of Encentrum walterkostei Jersabek is amended by observations on living animals. Of 19 species encountered, all but four are new to the alpine region, nine species are first records for the biogeographic region 'Alps'. Four species are new to science and will be published elsewhere. The majority are cosmopolites or widely distributed taxa, but also species with a more limited range, possibly endemics, seem to exist. Most species can be characterized as being more commonly found in cold environments, some of them are known to be psammobiontic or psammophilic.
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  • 84
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    Hydrobiologia 367 (1998), S. 175-187 
    ISSN: 1573-5117
    Keywords: Heteronemertea ; Yinia pratensis gen. et sp. nov. ; taxonomy ; Changjiang River Estuary
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A new genus and species of heteronemertean, Yinia pratensis gen. nov. and sp. nov., collected from low salinity waters (salinity 0.2–0.4 ‰) at Changjiang River Estuary, is described and illustrated. The species possesses a proboscis with an outer circular and an inner longitudinal muscle layer, and is placed in family Lineidae sensu Gibson. The following combination of morphological features distinguishes the new species from any other genera in this family: proboscis with two muscle crosses; dermis without connective tissue layer between gland cells and body wall outer longitudinal muscle layer; rhynchocoel wall circular muscles not interweaving with adjacent body wall longitudinal muscles; foregut with circular somatic muscles and subepithelial gland cell layer; neurochord cells present in central nervous system; caudal cirrus missing; blood system developed into alimentary plexus extending almost the full length of the body. Another significant character is that the lobular excretory cells are extremely well developed which may represent adaptation to water of low salinity.
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  • 85
    ISSN: 1573-5117
    Keywords: Rotifera ; Notholca ; taxonomy ; new species ; China
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    Notes: Abstract Notholca dongtingensis n.sp. was found in the second largest lake of China, Dongting Lake. It is related to Notholca labis Gosse, 1887 and N. kozhovi Vassilijewa & Kutikova, 1969. Its main distinguishing taxonomic features are a square-oval lorica, very short anterior spines, anterior lateral spines curving outwards and protrusile posterior margin of the ventral plate.
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  • 86
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    Hydrobiologia 387-388 (1998), S. 27-33 
    ISSN: 1573-5117
    Keywords: Rotifera ; biodiversity ; floodplain ; taxonomy ; Thailand
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A survey of 11 freshwater habitats in the floodplain of the River Nan, northern Thailand was carried out during April and September 1996. The rotifer samples were collected qualitatively from paddy fields, ponds, canals and reservoirs, using a 60 μm mesh net. One hundred and eighteen species were identified, four (Lepadella quinquecostata (Lucks), Macrochaetus danneeli Koste & Shiel, Testudinella ahlstromi Hauer and T greeni Koste) of which are new to Thailand and one (L. quinquecostata) is new to Asia. The numbers of species found in two localities are relatively high, with 86 and 73 rotifer taxa. Most of the species recorded are common, cosmopolitan or pantropical and warm-stenotherms. The occurrence of a species previously considered endemic to Australia, M. danneeli provides more evidence illustrating a relation between the rotifer faunas of southeast Asia and Australia. Comments are presented on some insufficiently known taxa in particular on the new records for Thailand.
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  • 87
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    Hydrobiologia 390 (1998), S. 171-217 
    ISSN: 1573-5117
    Keywords: taxonomy ; palaeoecology ; evolution ; punctuated equilibrium ; prey-predator coevolution ; bottlenecking ; thalassoidism ; zoogeography ; rift lakes ; lacustrine endemism ; Bellamya ; Neothauma ; Kaya
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract During late Cenozoic pre-rift times the viviparid genus Bellamya was probably confined to eastern Africa while in the Congo Basin occurred the genera Neothauma and Kaya (a new genus described herein). During the Pliocene, Kaya became extinct and Neothauma became a relict in Lake Tanganyika. All African rift lakes formed during or after the Pliocene were colonised by populations of Bellamya, which evolved into lacustrine endemics. The changes in shell morphology in Bellamya, such as ornamentation functioning as protection against predators, are modest and repetitive in time and space. After the initial adaptations stasis followed, and there is no indication of an arms race between prey and predator in this genus. In the lakes of the western rift that were formed prior to the Pliocene, namely Lake Tanganyika and Palaeolake Obweruka, Neothauma instead of Bellamya was the coloniser. Initially morphological inertia also occurred in this genus both in Tanganyika and, during the first four million years of its existence, in Palaeolake Obweruka, although from the outset this lake contained abundant highly specialised molluscivorous fishes. About 4.5 Ma a major extinction event occurred in the Obweruka Basin which led to the extermination of 50% of the molluscan species but none of the molluscivores. Among the viviparids, only one of the Neothauma species survived, its populations isolated and highly reduced in numbers. Immediately after this crisis a conchological quantum change occurred, the surviving lineage changing into a strongly ornamented thalassoid form. The dramatic morphological change is deemed to have been due to focussed selection by the predators on isolated and small prey populations. A radiative event immediately followed, producing still more strongly ornamented forms. The morphological changes that occurred in the Obwerukan Neothauma, in comparison to the modest and repeated patterns of evolution that can be observed in viviparids from other lakes, provides an example of true or quantum evolutionary change and yields evidence as to how it may have occurred. The fossil record of the fresh water molluscs of the Albertine Basin, thanks to its duration (ca. 12 million years), its relatively fine resolution (0.5–1.0 million years) and its sound chronostratigraphic framework, is a unique resource for understanding the tempo and mode of macroevolution.
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  • 88
    ISSN: 1573-5117
    Keywords: Cladocera ; chydorids ; semi-terrestrial plankton ; trunk limbs ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Two new chydorids, Nicsmirnovius camerounensis (gen. nov., sp. nov.) and Bryospilus africanus n.sp. are described, based on material from Korup, Mundemba, Bakingili and Debunscha, all in the rainforests of southwest Cameroon. Morphological structures show that both taxa are only remotely related, and that, while Nicsmirnovius is clearly an alonine, Bryospilus might well be a chydorine chydorid. Small, taxonomically non-significant differences occurred among specimens of Bryospilus recorded in three different forest areas.
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  • 89
    ISSN: 1573-5117
    Keywords: taxonomy ; new species ; leeches ; Helobdella ; Victoria ; Australia
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The first species of the genus Helobdella in Australia is recorded from Victoria. Helobdella papillornata has all of the diagnostic features of the genus including: triannulate segments, one pair of eyes on somite II, diffuse salivary glands, five crop caeca, one annulus between the gonopores and one annulus between the anus and the caudal sucker. It is characterised by a subterminal mouth, lobed gastric caeca, five to seven darkly pigmented papillae per somite on the dorsal surface, 14 brown dorsal stripes, five pairs of compact testisacs and a resting length of 15 mm and a maximum extended length of 40 mm. Individuals lay between 20 and 50 eggs attached directly to the ventral surface of the parent. At 20 °C the eggs hatch after 2 weeks and the young immediately reattach to the parent for a further 2 to 4 weeks, during which time the attached young feed on snails captured by the parent. After leaving the parent, young forage on their own or in small groups of three or more until they reach reproductive maturity. Individuals become reproductively mature, producing eggs and young, 3 to 4 months after hatching.
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  • 90
    ISSN: 1573-5117
    Keywords: ancient lake ; endemism ; brooding ; bivalves ; cementation ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A species from Lake Poso, Indonesia represents the first instance of cementation in the freshwater bivalve family Corbiculidae. Posostrea anomioides gen. nov., sp. nov., which is cemented indifferently by its left or right valve to calcareous rocks, reaches a size of 16 mm. It has typical corbiculoid dentition, with serrated lateral teeth, and juveniles are brooded primarily in the inner ctenidia. Lake Poso occupies a tectonic fault in central Sulawesi. Posostrea adds to an interesting radiation of endemic fishes and molluscs, including the gastropod genera Miratesta and Tylomelania.
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  • 91
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    Biodiversity and conservation 7 (1998), S. 847-868 
    ISSN: 1572-9710
    Keywords: corals ; diversity ; taxonomy ; Indian Ocean
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Corals from 26 sites in the Indian Ocean, from numerous taxonomic sources, are analysed for distribution patterns after applying a consistent synonymy. The data set contains double the amount of distribution information used previously. Cluster analysis shows several contiguous regional groupings within this ocean, and no geographically dispersed groupings exist. Between-site species similarity correlates negatively with distance between sites. Coral species diversity and genus diversity plotted with latitude and longitude confirm that a band of high diversity stretches across the entire Indian Ocean, but that in this Ocean, unlike the Pacific and greater Caribbean area, there is no clear gradient with latitude at least up to the latitudinal limits of coral growth. About half the species are widespread throughout the Indian Ocean. Species frequency/distribution curves and cumulative frequency curves show that 150 species occur at only 2–4 sites. Multi-dimensional scaling (MDS) analysis using only these species confirms that they are important in forming sub-regional groupings which are superimposed on a general Indian Ocean homogeneity. The perennial problem of error in taxonomic data sets is examined. Increasing taxonomic error is introduced into the data using random methods. It is found that about 25% more error can be added to this data set before the relationship between similarity coefficient and geographical distance between pairs of sites is lost. Measures extracted from the clustering procedure using the original data and the data sets with added taxonomic error, show a sharp loss in cluster formation after addition of about 10 or 20% more error.
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  • 92
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    Photosynthetica 35 (1998), S. 121-127 
    ISSN: 1573-9058
    Keywords: Gaussian distribution ; leaf age ; Triticum aestivum ; wheat
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Parameters of the fast chlorophyll (Chl) fluorescence induction (the O-J-I-P curve) of plants of winter wheat grown in the field canopy were statistically tested for Gaussian distribution. Five different statistical methods showed that the obtained values did not obey the Gaussian distribution law. The presentation of the parameters with the help of the mean and standard deviation masks the information about statistical properties of the values. Thus, we recommend to present the parameters by means of median, quartiles, and minimum and maximum values rather than by means of the mean and standard deviation.
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  • 93
    ISSN: 1573-9058
    Keywords: cytokinin ; drought ; proteins ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The carboxylating activity and content of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCO, EC 4.1.1.39), and other soluble proteins in young seedlings and mature leaves of Lutescens-758, a drought-sensitive cultivar of soft spring wheat Triticum aestivum L., were studied under the conditions of drought and subsequent rehydration. Seedlings and mature plants preliminarily treated with the cytokinin-like compound kartolin-4 were compared to untreated plants. Drought-induced decrease in RuBPCO activity should be attributed not only to proteolytic decomposition of the enzyme protein itself but also to a partial inhibition of its catalytic activity. The decrease in RuBPCO activity was larger than that in RuBPCO content. Water stress induced a marked decrease in the soluble protein content. Kartolin-4 increased the resistance to drought.
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  • 94
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    World journal of microbiology and biotechnology 14 (1998), S. 705-709 
    ISSN: 1573-0972
    Keywords: Fermentation ; polyene antibiotic ; Streptoverticillium ; sclerotia ; taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Abstract A novel Streptoverticillium sp. G-55 was isolated from a soil sample (collected from Panjim, Goa) which produces sclerotia under specific environmental conditions, both in liquid and solid media. It was further identified by taxonomic studies as Streptoverticillium cinnamoneum var scleroticum. The species produces a pentaene polyene macrolide antibiotic (HA-94) under submerged culture conditions which shows promising antifungal and antibacterial activity in vitro.
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  • 95
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    European journal of plant pathology 104 (1998), S. 37-48 
    ISSN: 1573-8469
    Keywords: cultural practices ; disease progress equation ; infection cycles ; Pseudocercosporella herpotrichoides ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Wheat was assessed at four crop growth stages for eyespot (anamorph Pseudocercosporella herpotrichoides, teleomorph Tapesia yallundae) in a series of field trials that studied the effects on disease frequency of five wheat management techniques (sowing date and density, nitrogen fertiliser dose and form, removal/burial of cereal straw). An equation expressing disease level as a function of degree-days was fitted to the observed disease levels. This equation was based on eyespot epidemiology and depended on two parameters illustrating the importance of the primary and the secondary infection cycles respectively. Cultural practices were classified according to the importance of their effects on disease, and these effects could be related to infection cycles and host plant architecture. Sowing date had the earliest and strongest effect; early sowing always increased disease frequency through the primary infection cycle, and its influence on the secondary cycle was variable. Disease frequency was increased by high plant density and/or a low shoot number per plant through primary infection; the secondary cycle was, however, decreased by a low shoot number per plant, which reduced late disease development at high plant density. High nitrogen doses increased disease levels and the severity of both infection cycles, but this effect was partly hidden by a simultaneous stimulation of tillering and thus an indirect decrease of disease incidence. When significant, ammonium (vs ammonium nitrate) fertiliser decreased eyespot levels and infection cycles whereas straw treatment (burial vs removal of straw from the previous cereal crop) had no effect.
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  • 96
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    Biologia plantarum 41 (1998), S. 427-434 
    ISSN: 1573-8264
    Keywords: phloem ; rubidium ; strontium ; Triticum aestivum ; xylem
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Detached wheat shoots (ear with peduncle and flag leaf) were incubated for 4 d in a solution containing 1 mM RbCl and 1 mM SrCl2 as well as 10, 40 or 160 µM NiCl2 and CoCl2. The phloem of some plants was interrupted by steam-girdling the stem below the ear to distinguish between xylem and phloem transport. The phloem-immobile Sr flowed mainly to the leaf lamina and to the glumes via the xylem. The Sr transport was not sensitive to steam-girdling. In contrast, the phloem-mobile Rb accumulated during the incubation time mainly in the stem and the leaf sheath. The Rb transport to the grains was impaired by steam-girdling as well as by elevated Ni and Co concentrations in the incubation solution indicating that Rb was transported via the phloem to the maturing grains and that this transport was affected by the heavy metals. Ni was removed more efficiently from the xylem in the peduncle than Co (but far less efficiently than Rb). It became evident that the two heavy metals can also be transferred from the xylem to the phloem in the stem of wheat and reach the maturing grains via the phloem.
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  • 97
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    Biologia plantarum 41 (1998), S. 387-394 
    ISSN: 1573-8264
    Keywords: active oxygen species ; ascorbate peroxidase ; ascorbic acid ; catalase ; hydrogen peroxide ; oxidative injury ; oxidative stress ; superoxide dismutase ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The role of plant antioxidant systems in stress tolerance was studied in leaves of three contrasting wheat genotypes. Drought imposed at two different stages after anthesis resulted in an increase in H2O2 accumulation and lipid peroxidation and decrease in ascorbic acid content. Antioxidant enzymes like superoxide dismutase, ascorbate peroxidase and catalase significantly increased under water stress. Drought tolerant genotype C 306 which had highest ascorbate peroxidase and catalase activity and ascorbic acid content also showed lowest H2O2 accumulation and lipid peroxidation (malondialdehyde content) under water stress in comparison to susceptible genotype HD 2329 which showed lowest antioxidant enzyme activity and ascorbic acid content and highest H2O2 content and lipid peroxidation. HD 2285 which is tolerant to high temperature during grain filling period showed intermediate behaviour. Superoxide dismutase activity, however, did not show significant differences among the genotypes under irrigated as well as water stress condition. It seems that H2O2 scavenging systems as represented by ascorbate peroxidase and catalase are more important in imparting tolerance against drought induced oxidative stress than superoxide dismutase alone.
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  • 98
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    Biologia plantarum 41 (1998), S. 65-73 
    ISSN: 1573-8264
    Keywords: ascorbate peroxidase ; catalase ; flavonoids ; superoxide dismutase ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In wheat seedlings (Triticum aestivum L. cv. 2329) oxidative stress caused by UV-B radiation led to lipid peroxidation of thylakoid membrane; it was expressed in term of malondialdehyde (MDA) formation. The peroxidation of lipids of thylakoid membrane in isolated chloroplasts was prevented when flavonoids quercetin and rutin were supplied into the incubation medium. The activities of superoxide dismutase, ascorbate peroxidase, and catalase increased during the first hours of UV-B exposure. A comparative study of UV-B and temperature effects showed different profiles of the antioxidant enzymes and MDA, suggesting that these two stresses have distinct sites of action. In addition to quantitative increase in flavonoids, qualitative change in flavonoid composition was also marked during UV-B stress, and a new peak at 330 nm was found as compared to control.
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  • 99
    ISSN: 1573-8264
    Keywords: Brassica napus ; ex vitro acclimation ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The greatest growth of wheat and rape plants in vitro was reached on media with 5 or 9 % sucrose, respectively. The highest efficiency for transfer of these plants to ex vitro conditions was found at the same sucrose concentrations. The content of endogenous non-structural saccharides (glucose, fructose, sucrose, starch and fructans) increased with increasing sucrose concentration in the medium up to 10 %.
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  • 100
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    Biologia plantarum 41 (1998), S. 287-292 
    ISSN: 1573-8264
    Keywords: enzyme activities ; liquid culturing ; protein ; starch ; sugars ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Effect of fluoride (10 and 50 mM) on the activities of sucrose metabolizing enzymes, alkaline inorganic pyrophosphatase, and transaminases in relation to the accumulation of free sugars, starch, and soluble protein was studied in detached ears of wheat cultured in a liquid medium. Culturing for 5 d in the presence of fluoride reduced the amount of grain starch whereas contents of total free sugars, particularly sucrose, and soluble protein increased. Fluoride inhibited the activities of soluble acid and neutral invertases, as well as sucrose synthase acting in the cleavage direction. Uptake of uniformly labelled 14C-sucrose or fructose was also drastically reduced by fluoride. Glutamate-oxaloacetate transaminase (GOT) and glutamate-pyruvate transaminase (GPT) activities also increased with fluoride addition in correspondence with an increase in soluble protein. Apparently, the wheat grain responds to fluoride-mediated disruption of carbon metabolism by a compensatory effect on nitrogen metabolism.
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