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  • 1
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    Springer
    Biochemical genetics 27 (1989), S. 551-569 
    ISSN: 1573-4927
    Keywords: mitochondrial DNA (mtDNA) ; restriction fragment analysis ; genetic variation ; screwworm fly ; Diptera ; Cochliomyia hominivorax
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Mitochondrial DNA variability has been analyzed in the primary screwworm fly (Cochliomyia hominivorax) using restriction endonuclease fragment patterns and restriction site mapping. A total of 30 different screwworm lines originating from Texas to Costa Rica and the Island of Jamaica was examined using 15 restriction endonucleases. Eleven of the restriction enzymes revealed polymorphism and yielded 16 mitochondrial genotypes or haplotypes. Two of the haplotypes were widely distributed, haplotype 1 being found scattered across southern Mexico and haplotype 2 along the west coast of Mexico. Haplotype 1 also appeared paired with several other haplotypes in mixed lines that were most likely the result of collecting an egg mass to which more than one female had contributed or to some form of contamination by haplotype 1 after introduction into the laboratory. These lines became fixed before single insects were examined and thus it is impossible to rule out heteroplasmy. The other 14 haplotypes were found in only a single locale and 12 of these were found in only one line. The average sequence diversity among 27 mainland lines was about 0.5%. The two Jamaican lines and one east coast mainland line differed from the others by 〉2%. The pattern of geographical distribution, a small number of apparently recurring haplotypes and a substantial number (75%) of the haplotypes unique, bears similarities to patterns observed in other insects such asDrosophila. The high frequency of unique genotypes in southern Mexico suggests a population with a very reduced gene flow, which may have had a positive effect on the sterile male release control program.
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  • 2
    Electronic Resource
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    Biochemical genetics 27 (1989), S. 551-569 
    ISSN: 1573-4927
    Keywords: mitochondrial DNA (mtDNA) ; restriction fragment analysis ; genetic variation ; screwworm fly ; Diptera ; Cochliomyia hominivorax
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Mitochondrial DNA variability has been analyzed in the primary screwworm fly (Cochliomyia hominivorax) using restriction endonuclease fragment patterns and restriction site mapping. A total of 30 different screwworm lines originating from Texas to Costa Rica and the Island of Jamaica was examined using 15 restriction endonucleases. Eleven of the restriction enzymes revealed polymorphism and yielded 16 mitochondrial genotypes or haplotypes. Two of the haplotypes were widely distributed, haplotype 1 being found scattered across southern Mexico and haplotype 2 along the west coast of Mexico. Haplotype 1 also appeared paired with several other haplotypes in mixed lines that were most likely the result of collecting an egg mass to which more than one female had contributed or to some form of contamination by haplotype 1 after introduction into the laboratory. These lines became fixed before single insects were examined and thus it is impossible to rule out heteroplasmy. The other 14 haplotypes were found in only a single locale and 12 of these were found in only one line. The average sequence diversity among 27 mainland lines was about 0.5%. The two Jamaican lines and one east coast mainland line differed from the others by 〉2%. The pattern of geographical distribution, a small number of apparently recurring haplotypes and a substantial number (75%) of the haplotypes unique, bears similarities to patterns observed in other insects such asDrosophila. The high frequency of unique genotypes in southern Mexico suggests a population with a very reduced gene flow, which may have had a positive effect on the sterile male release control program.
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  • 3
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    Journal of chemical ecology 15 (1989), S. 719-730 
    ISSN: 1573-1561
    Keywords: Onion fly ; Delia antiqua ; Diptera ; Anthomyiidae ; oviposition ; deterrent ; capsaicin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract In laboratory choice experiments, the spices dill, paprika, black pepper, chili powder, ginger, and red pepper deterredDelia antiqua oviposition by 88–100%. Dose-response choice tests demonstrated that 1 mg of ground cayenne pepper (GCP) placed within 1 cm of artificial onion foliage reduced oviposition by 78%. A synthetic analog of capsaicin, the principal flavor ingredient of red peppers, deterred oviposition by 95% when present at 320 ppm in the top centimeter of sand (the ovipositional substrate). However, in no-choice conditions 10 mg GCP was not an effective deterrent. Sevana Bird Repellent and Agrigard Insect Repellent both use red pepper as a principal ingredient; at recommended field rates, neither of these materials was an effective ovipositional deterrent either in laboratory or field. Capsaicin-based materials do not appear to be candidates for onion maggot control via behavioral modification.
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  • 4
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    Journal of chemical ecology 15 (1989), S. 1045-1055 
    ISSN: 1573-1561
    Keywords: Drosophila ananassae ; Drosophila bipectinata ; Diptera ; Dro-sophilidae ; aggregation pheromone ; (Z)-11-octadecenyl acetate ; (Z)-11-eicosenyl acetate ; cis-vaccenyl acetate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract (Z)-11-Octadecenyl acetate (Z11–18∶Ac) and (Z)-11-eicosenyl acetate (Z11–20∶Ac) were identified as the aggregation pheromones ofDrosophila ananassae, andZ11–20∶Ac was identified as the aggregation pheromone ofDrosophila bipectinata. Z11–18∶Ac andZ11–20∶Ac were not attractive alone; however, in combination with fermenting food odors, the acetates attracted flies of both sexes in a wind-tunnel olfactometer. The pheromones were present in the ejaculatory bulb of sexually mature male flies and transferred to the female during mating. MaleD. bipectinata released little if anyZ11–20∶Ac to the food; however, recently mated females releasedZ11–20∶Ac to the surrounding surfaces in just a few hours after mating.D. ananassae males, on the other hand, appeared to release moreZ11–18∶Ac andZ11–20∶Ac to the surroundings than mated females. AlthoughD. bipectinata males had noZ11–18∶Ac, flies were as attracted toZ11–18∶Ac as to an equal quantity ofZ11–20∶Ac.D. ananassae were attracted toZ11–18∶Ac but not toZ11–16∶Ac orZ11–20∶Ac. However,Z11–20∶Ac in combination withZ11–18∶Ac was significantly more attractive thanZ11–18∶Ac alone.
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  • 5
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    Journal of chemical ecology 15 (1989), S. 1231-1239 
    ISSN: 1573-1561
    Keywords: Graphite furnace ; heavy metals ; chemoprints ; Delia radicum ; Diptera ; Anthomyiidae ; surface adsorption ; oxidation states ; metal cations
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Graphite furnace and flame atomic absorption spectroscopy were used to make quantitative determinations of a range of metallic cation contents of wet-ashed female individuals ofDelia radicum (L.) from a laboratory culture reared under controlled conditions and freeze-dried upon emergence. Analyses were done for seven elements: Fe, Zn, Cu, Pb, AI, Mg, and K. The quantities of K and Mg present were positively related to the dry weight of individual flies, while the others showed an exponential decrease in concentration with increasing fly weight. This difference is attributed to the different roles of surface adsorption of metals with higher oxidation states and the high absorption efficiencies of those existing as oxidation states 1 and 2. The weight of the insect is therefore a major factor in determining the individual's chemoprint. The implications of this observation for other studies are discussed.
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  • 6
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    Journal of chemical ecology 15 (1989), S. 1423-1432 
    ISSN: 1573-1561
    Keywords: Courtship ; pheromones ; (Z,Z)-7,11-heptacosadiene ; Drosophila rajasekari ; Drosophila melanogaster ; Diptera ; Drosophilidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The courtship behaviors and cuticular hydrocarbons ofDrosophila rajasekari are described. Sexually mature males orient, tap, follow, vibrate their abdomens, extend and vibrate their wings, and attempt copulation during courtship. They perform these behaviors in response to immature and matureD. rajasekari of both sexes, and their courtship activities are facilitated by light. The predominant cuticular hydrocarbon found in both sexes is (Z,Z)-7,11-heptacosadiene (HCD), a compound known to be used as a courtship-stimulating sex pheromone by another fruit fly,D. melanogaster. Therefore, it is not surprising thatD. melanogaster males actively court both males and females from theD. rajasekari stock. However, HCD is apparently not used byD. rajasekari as a courtship-stimulating pheromone since matureD. rajasekari males do not courtD. melanogaster females, which produce large quantities of HCD.
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  • 7
    ISSN: 1573-1561
    Keywords: Housefly ; Musca domestica ; Diptera ; Muscidae ; sex pheromone ; (Z)-9-tricosene ; (Z)-9-alkenes ; n-alkanes ; monomethyalkanes ; dimethylalkanes ; jojoba wax
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Mixtures of (Z)-9-alkenes (C21-C33),n-alkanes (C21-C33), and mono- and dimethylalkanes (C27, C29), as components of the cuticular lipids washed from the female housefly (Musca domestica L.), were synthesized and assayed for their biological activity on male houseflies. The (Z)-9-alkenes and their hydrogenatedn-alkanes were synthesized from jojoba wax components by the appropriate chain elongation. The methylalkanes were prepared by Grignard coupling reaction of the appropriate alkyl halides, catalyzed by Li2CuCl4. Six- to 7-day-old virgin male houseflies exhibited the highest mating strike activity toward 6- to 7-day-old virgin females. The mating strike activity of the synthetic hydrocarbons was studied by exposing 6- to 7-day old virgin males to petrol-ether-rinsed 1 to 2-day-old dead females treated with these chemicals. (Z)-9-Tricosene was the most active hydrocarbon tested when it was applied in amounts of 10 μg to a washed dead female. Amounts of 5 μg of (Z)-9-tricosene did not cause sexual activity in the males. Mixture of (Z)-9-alkenes showed low activity when applied at 10-μg amounts. However, when mixtures were made of 5 μg of (Z)-9-tricosene with 5 fig of each of certain mixtures of (Z)-9-alkenes, the striking activity became as high as that of 10 μg of (Z)-9-tricosene. This might be a synergism effect. The mixtures ofn-alkanes, as well as the mono- and dimethylalkanes, showed moderate activity. When (Z)-9-tricosene was added to these materials no increase in male activity was observed.
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  • 8
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    Journal of chemical ecology 15 (1989), S. 1559-1566 
    ISSN: 1573-1561
    Keywords: (Z)-11-Hexadecenal ; (Z)-11-hexadecen-1-ol ; (Z)-9-tetradecenal ; iris ; iris borer ; Macronoctua onusta ; tobacco budworm ; Heliothis virescens ; Lepidoptera ; Noctuidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Chromatographic and mass spectrometry studies of heptane extracts of the ovipositors of the iris borer,Macronoctua onusta, showed that the females produce several compounds that are the same as those produced by females of the tobacco budworm,Heliothis virescens. In trapping experiments, a mixture of (Z)-11-hexadecenal, (Z)-11-hexadecen-1-ol, and (Z)-9-tetradecenal (94∶4∶2) proved to be the minimum set of compounds required to cause effective capture of iris borer males in the field.
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  • 9
    ISSN: 1573-1561
    Keywords: Trap color ; trap design ; velvetbean caterpillar ; Anticarsia gemmatalis ; fall armyworm moth ; Spodoptera frugiperda ; Lepidoptera ; Noctuidae ; electroretinogram
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Three pheromone traps were evaluated in paired field trials for effectiveness in capturing wild male velvetbean caterpillar moths (VBC),Anticarsia gemmatalis Hübner, and fall armyworm moths (FAW),Spodoptera frugiperda (J.E. Smith), using a wind-oriented trapping device. The traps were: (1) the standard multicolored bucket trap consisting of a forest green canopy, yellow funnel, white bucket and open (i.e., single wire) pheromone holder; (2) a forest green monocolored bucket trap; and (3) the Multi-Pher-1 trap consisting of a blue-green canopy, white funnel, white bucket, and white, multislotted pheromone holder. The Multi-Pher-1 trap differs primarily from the other two in that its entrance is smaller in diameter and is partially obstructed by the pheromone holder. Significantly fewer VBC and FAW males were captured in pheromone-baited monocolored (forest green) bucket traps than standard multicolored bucket traps. The Multi-Pher-1 trap also caught significantly fewer VBC moths than the standard multicolored bucket trap, but there was no significant difference in numbers of FAW moths caught in the two traps. The results further demonstrate the importance of considering visual cues, in this case color, in the design of pheromone traps for nocturnal insects.
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  • 10
    ISSN: 1573-1561
    Keywords: Enedial ; Spodoptera ; Lepidoptera ; Noctuidae ; feeding deterrent ; pyrrole ; sulfhydryl ; van der Waals interaction ; Reimer-Tiemann adducts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Structure-activity relationships of naturally occurring enedials with antifeedant activity againstSpodoptera species have been extended via the synthesis and bioassay of a series of Reimer-Tiemann adducts. The activities attributed to the different chemical structures of these and other analogs interacting with the chemoreceptor site have been observed; a three-pronged mode of substrate binding via aromatic pyrrole formation, Michael addition of free sulfhydryl moieties, and van der Waals interactions of the A ring has been postulated to account for these observations.
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  • 11
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    Journal of chemical ecology 15 (1989), S. 2301-2317 
    ISSN: 1573-1561
    Keywords: Insect ; screwworm ; Cochliomyia hominivorax ; Diptera ; Calliphoridae ; cuticular hydrocarbons ; mass spectrometry ; 2,X-dimethylalkanes
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract A novel series of 2,X-dimethylalkanes were isolated and identified. The nonpolar fraction of the surface lipids secreted by the adult (5-day-old) screwworm,Cochliomyia hominivorax, contains over 130 different hydrocarbons comprising normal alkanes (32% of the total hydrocarbon), branched alkanes (53%), and monoalkenes (11%). Branched alkanes included monomethylalkanes with substitution in all possible positions except for 4-methylalkanes, internally branched dimethylalkanes, and 2,X- and 3,X-dimethylalkanes. At emergence, adults of both sexes of the 009 strain have nearly identical gas Chromatographic profiles, which diverge as the insect ages. Irradiation of pharate pupae does not affect the hydrocarbon produced.
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  • 12
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    Journal of chemical ecology 15 (1989), S. 2379-2390 
    ISSN: 1573-1561
    Keywords: Acidic fog ; Phaseolus lunatus ; Trichoplusia ni ; cabbage looper ; Lepidoptera ; Noctuidae ; nitrogen ; free amino acid ; soluble protein ; plantinsect interactions ; air pollution
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Phaseolus lunatus L. (Henderson Bush lima beans) were exposed to 2 hr acidic fogs with 2.5∶1.0 (v/v) nitrogen-sulfur ratio typical of the west coast of the United States. Fogs with pH values of 2.0 (P 〈 0.01,t tests), 2.5 (P 〈 0.05), or 3.0 (P 〈 0.01) increased percent total nitrogen (dry weight) of foliage as compared to plants subjected to control fogs with a pH of 6.3–6.5. Fresh weight concentrations of soluble protein and certain free amino acid concentrations were increased by plant exposure to acidic fogs with a pH of 2.5 (t tests,P 〈 0.05). Concentrations of free amino acids considered essential for insect growth, as well as nonessential and total free amino acids were not significantly affected by any treatment (P 〉 0.05,t test). Water content (%) of foliage was not changed significantly (P 〉 0.05,t test) by exposure to any of the fogs.Trichoplusia ni (Hübner) (Lepidoptera: Noctuidae) larvae ate significantly more foliage and gained significantly more weight on plants treated with 3.0 pH fogs (P 〈 0.01,t test). Several potential explanations are offered for the lack of significant weight gain by larvae on plants in which soluble protein levels, free amino acid concentrations, or percent total nitrogen contents were enhanced by acidic fogs with a pH of 2.5 and 2.0. No larval feeding preference was detected for foliage exposed to acidic versus control fogs, and no significant differences were detected in percent survival ofT. ni eggs exposed to acidic or control fogs. Some implications of acidic fogs for population dynamics ofT. ni are discussed.
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  • 13
    ISSN: 1573-1561
    Keywords: Olive fruit fly ; Dacus oleae ; Diptera ; Tephritidae ; pheromones ; attractants ; sex attractants ; pheromone analog ; 1,5,7-triox-aspiro[5.5]undecane
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract l,5,7-Trioxaspiro[5.5]undecane, an analog of the major sex pheromone (olean) of the olive fruit fly, was synthesized via two different routes and tested for biological activity under laboratory and field conditions. In laboratory tests, its activity was comparable to that of olean, especially when a stabilizer or a high concentration was used. In field tests, its activity reached the level of olean only when a stabilizer and an open-type dispenser, which allows high rates of evaporation, were used. The residual activity of the analog dispensers did not exceed two weeks both under laboratory and field conditions compared to over four months for olean.
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  • 14
    ISSN: 1573-1561
    Keywords: Pseudaletia unipuncta ; Lepidoptera ; Noctuidae ; hairpencils ; male pheromone ; acetic acid ; benzaldehyde ; benzyl alcohol ; antennal ; response
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Hairpencil secretion ofPseudaletia unipuncta (Haw.) contains acetic acid as well as previously identified benzaldehyde and benzyl alcohol. Age-specific titers of acetic acid were significantly greater than those of benzaldehyde and, at 25 °C, accumulation of both compounds in the hairpencils peaked on the second day after emergence. Excised antennae of males and females perceived both compounds. Antennal response to acetic acid did not vary significantly with age or sex, but male response to benzaldehyde was significantly greater than female response at all ages tested. Antennal response of both males and females to benzaldehyde tended to be greater on the fourth and eighth days after emergence than on the second.
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  • 15
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    Journal of chemical ecology 15 (1989), S. 169-182 
    ISSN: 1573-1561
    Keywords: Balansiae ; Clavicipitaceae ; ergot alkaloids ; fall armyworm ; feeding deterrents ; acquired chemical defense ; fungal endophytes ; grasses ; Lepidoptera ; Noctuidae ; Spodoptera frugiperda
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Ergot alkaloids produced by endophytic fungi in the tribe Balansiae (Clavicipitaceae, Ascomycetes), which infect grasses, may provide plant defense against herbivores. This study examined the effects of six ergot alkaloids on survivorship, feeding, and growth of larvae of the fall armyworm (Spodoptera frugiperda, Lepidoptera: Noctuidae), a generalist herbivore of grasses. Corn leaf disks were soaked in solutions of individual ergot alkaloids at different concentrations and presented to neonate larvae. At the highest concentrations (77–100 mg/liter) of ergonovine, ergotamine, ergocryptine, agroclavine, and elymoclavine, larval weights and/or leaf area consumed after eight days were reduced relative to controls. Lysergol had no effect on larval weights and leaf consumption at any concentration. Although active concentrations were higher than those reported from two host grasses, in vivo levels of ergot alkaloids have not been quantified for most endophyte-infected grasses. The detrimental effects on fall armyworm observed in this study suggest that ergot alkaloids could be responsible, at least in part, for the greater insect resistance of endophyte-infected grasses.
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  • 16
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    Journal of chemical ecology 15 (1989), S. 249-254 
    ISSN: 1573-1561
    Keywords: Fusaric acid ; corn earworm ; Heliothis zea ; Lepidoptera ; Noctuidae ; saponin ; gossypol ; 6-methoxy-2-benzoxazolinone ; synergism ; allelochemical ; mycotoxin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The ability of naturally occurring levels of theFusarium spp. fungal metabolite fusaric acid to synergize the toxicity of the allelochemicals gossypol, a saponin, and 6-methoxy-2-benzoxazolinone to larvae ofHeliothis zea (Boddie) was tested. Levels of fusaric acid comparable to those found near the fungus increased mortality ofH. zea to gossypol, the saponin, and 6-methoxy-2-benzoxazolinone, and decreased the development rate of surviving larvae exposed to gossypol and 6-methoxy-2-benzoxazolinone. Some effect was also noted for levels of fusaric acid found generally distributed throughout infected plants. The chemical properties of fusaric acid suggest that it synergizes the toxicity of the allelochemicals by inhibiting oxidative enzymes responsible for detoxification. Production of the biosynthetically simple fusaric acid may be a fungal strategy for conserving resources as compared to those fungi that produce biosynthetically complex toxins of their own.
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  • 17
    ISSN: 1573-1561
    Keywords: Heliothis virescens ; Lepidoptera ; Noctuidae ; hairpencil glands ; male-produced compounds ; hexadecanyl acetate ; hexadecanol ; (Z)-11-hexadecenyl acetate ; octadecanyl acetate ; octadecanol ; tetradecanoic acid ; hexadecanoic acid ; octadecanoic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Extracts of the intact hairpencil glands and hairs from the hairpencil glands of males ofHeliothis virescens (F.) were analyzed by capillary gas chromatography (GC) and by GC-mass spectroscopy. These analyses indicated that hexadecanyl acetate (212.4 ng/male), hexadecanol (22.3 ng/male), (Z)-11-hexadecenyl acetate (3.5 ng/male), octadecanyl acetate (14.2 ng/male) octadecanol (7.5 ng/male), tetradecanoic acid (2.7 ng/male), hexadecanoic acid (22.3 ng/male), and octadecanoic acid (6.5 ng/male) were present in the extracts. These compounds also were found in volatiles released from the hairpencil glands. In addition, GC analysis using both polar and apolar capillary columns indicated that extracts of the glands and extracts of the hairs from the hairpencil glands contained small amounts of tetradecanyl acetate, (Z)-9-tetradecenyl acetate, tetradecanol, (Z)-7-hexadecenyl acetate, (Z)-9-hexadecenyl acetate, and (Z)-11-hexadecenol. No (Z)-9-tetradecenal was present. Studies indicated that titers of the compounds increased rapidly during the 36 hr after adult eclosion and then leveled off, being maintained at high levels until released when the glandular hairs were exposed. During exposure of the hair pencils substantial amounts of the compounds were released. In vivo application of 500 ng of suspensions of (Z)-11-hexadecenyl acetate, (E)-11-hexadecenyl acetate, (Z)-11-tetradecenyl acetate, or (E)-11-tetradecenyl acetate in dimethyl sulfoxide to the surface of the denuded hairpencil gland showed that biosynthesis proceeds to the alcohol via the acetate.
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  • 18
    ISSN: 1573-1561
    Keywords: Polyphenol oxidase ; peroxidase ; digestibility reduction ; plant-insect interactions ; phenolic-protein binding ; chlorogenic acid ; Heliothis zea ; Spodoptera exigua ; Lepidoptera ; Noctuidae ; Lycopersicon esculentum ; host-plant resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The foliage and fruit of the tomato plantLycopersicon esculentum contains polyphenol oxidases (PPO) and peroxidases (POD) that are compartmentally separated from orthodihydroxyphenolic substrates in situ. However, when leaf tissue is damaged by insect feeding, the enzyme and phenolic substrates come in contact, resulting in the rapid oxidation of phenolics to orthoquinones. When the tomato fruitwormHeliothis zea or the beet army-wormSpodoptera exigua feed on tomato foliage, a substantial amount of the ingested chlorogenic acid is oxidized to chlorogenoquinone by PPO in the insect gut. Additionally, the digestive enzymes of the fruitworm have the potential to further activate foliar oxidase activity in the gut. Chlorogenoquinone is a highly reactive electrophilic molecule that readily binds cova-lently to nucleophilic groups of amino acids and proteins. In particular, the —SH and —NH2 groups of amino acids are susceptible to binding or alkylation. In experiments with tomato foliage, the relative growth rate of the fruitworm was negatively correlated with PPO activity. As the tomato plant matures, foliar PPO activity may increase nearly 10-fold while the growth rate of the fruitworm is severely depressed. In tomato fruit, the levels of PPO are highest in small immature fruit but are essentially negligible in mature fruit. The growth rate of larvae on fruit was also negatively correlated with PPO activity, with the fastest larval growth rate occurring when larvae fed on mature fruit. The reduction in larval growth is proposed to result from the alkylation of amino acids/protein byo-quinones, and the subsequent reduction in the nutritive quality of foliage. This alkylation reduces the digestibility of dietary protein and the bioavailability of amino acids. We believe that this mechanism of digestibility reduction may be extrapolatable to other plant-insect systems because of the ubiquitous cooccurrence of PPO and phenolic substrates among vascular plant species.
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  • 19
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    Journal of chemical ecology 15 (1989), S. 309-315 
    ISSN: 1573-1561
    Keywords: Xanthotoxin ; Heliothis zea ; Lepidoptera ; Noctuidae ; myristicin ; fagaramide ; safrole ; isosafrole ; synergism ; phytosynergist ; phototoxicity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The methylenedioxyphenyl-containing (MDP) inhibitors of mixed-function oxidase detoxification enzymes, myristicin, safrole, fagaramide, and isosafrole, occur with xanthotoxin or other toxic furanocoumarins in plants of the families Umbelliferae and Rutaceae. All four MDP compounds have a synergistic effect on the toxicity of xanthotoxin toHeliothis zea. Myristicin also increased the phototoxicity of xanthotoxin in the presence of UV light. The term phytosynergist is used to describe plant compounds that are present at concentrations producing no toxic effect by themselves but have a synergistic effect on cooccurring toxins.
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  • 20
    ISSN: 1573-1561
    Keywords: Lepidoptera ; Noctuidae ; Heliothis zea ; Spodoptera exigua ; Lycopersicon esculentum ; tomato ; saponin ; glycoalkaloid ; tomatine ; cholesterol ; developmental sensitivity ; nutritional indices ; allelochemical interactions ; host-plant resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The effect of tomatine on larval growth ofHeliothis zea andSpodoptera exigua was assessed by rearing larvae on diets with different concentrations of the chemical added. When reared from neonates, linear dose-response relationships were obtained for both species, withS. exigua being three times more sensitive to tomatine thanH. zea. Tomatine toxicity was completely alleviated inH. zea by the addition of equimolar cholesterol into the diet; however, inS. exigua some toxicity was maintained. Larvae ofS. exigua that were started on control diet were insensitive to tomatine after five days; larvae started on diet with an EC50 of tomatine and then switched to control diet after five days failed to recover from toxicosis. Larval growth ofH. zea, on the other hand, was affected both at the neonate and third-instar stage, but normal growth resumed when the larvae were transferred to control diet. Tomatine had little or no affect on food consumption, assimilation, or dietary utilization of the food by third-instar larvae ofS. exigua, except at a concentration 10 times the EC50. In contrast, the efficiency of food utilization ofH. zea larvae decreased with increasing tomatine concentrations. Assimilation of the food tended to increase, although not significantly, as tomatine levels increased. Food consumption ofH. zea larvae also increased when the tomatine concentration was greater than an EC50. The addition of equimolar cholesterol to diets with an EC50 of tomatine restored weight gain and nutritional indices values to control values. These results are related to the utility of using tomatine in host-plant resistance programs.
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  • 21
    ISSN: 1573-1561
    Keywords: Corn earworm ; Heliothis zea ; Lepidoptera ; Noctuidae ; pheromone ; (Z)-11-hexadecen-1-ol ; laboratory rearing
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Analysis of ovipositor extracts of lab-reared (〉 120 generations) and wildHeliothis zea indicated small but significant differences in the percent composition of the four aldehyde components of the sex pheromone. (Z)-11-hexadecen-1-ol (Z11–16∶OH) was present in both populations and amounted to only 2.8% of the total pheromone. There was no significant difference in four of the six behavioral categories between lab-reared and wild males exposed to the four-component sex pheromone in the flight tunnel. Ninety and 84% males flew to the pheromone source, respectively. However, with the addition ofZ11–16∶OH to the pheromone blend, none of the wild males flew upwind to the stimulus source, whereas 36% of the lab-reared males completed the flight. It is suggested that prolonged inbreeding in closed quarters rendered these males less discriminating to qualitative differences in a pheromone blend.
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  • 22
    ISSN: 1573-1561
    Keywords: Drosophila mauritiana ; Drosophila yakuba ; Drosophila rajasekari ; Diptera ; Drosophilidae ; (Z)-11-octadecenyl acetate ; cis-vaccenyl acetate ; aggregation pheromone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract (Z)-11-Octadecenyl acetate (Z11–18∶Ac) was identified as the aggregation pheromone ofDrosophila mauritiana, D. yakuba, andD. rajasekari. The amount of pheromone in the ejaculatory bulb of male flies increased with age, reaching plateau levels of ca. 240, 800, and 1100 ng/fly forD. mauritiana, D. yakuba, andD. rajasekari, respectively. Thirty to 50% of the pheromone in the ejaculatory bulb was transferred to the female during mating, with the majority transferred to the female's reproductive tract. In the subsequent 6-hr period, over half the pheromone in the female's reproductive tract was transferred to the surroundings. In a wind-tunnel olfactometer,Z11–18∶Ac was attractive toD. yakuba andD. mauritiana; however,D. rajasekari required food or food odors in combination withZ11–18∶Ac to demonstrate aggregation activity.Z11–16∶Ac andZ11–20∶Ac were not attractive forD. mauritiana, D. yakuba, andD. rajasekari. For all three species, food was synergistic withZ11–18∶Ac and both sexes were attracted.
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  • 23
    ISSN: 1573-1561
    Keywords: Hydraecia micacea ; Lepidoptera ; Noctuidae ; potato stem borer ; sex pheromones ; field trapping ; reproductive behavior ; tetradecenyl acetate ; (Z)-9-tetradecenyl acetate ; (E)-11-tetradecenyl acetate ; (Z)-11-tetradecenyl acetate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Behavior of males ofHydraecia micacea (Esper) responding to virgin females and to synthetic pheromone blends were investigated in a laboratory wind tunnel. The synthetic blend consisted of saturated 14∶Ac (68.9%),Z9–14∶Ac (3.4%),E11–14∶Ac (14.6%), andZ11–14∶Ac (13.1%). Virgin females were significantly better lures than the four-component synthetic blend for most behaviors. By deleting components individually from the four-component blend,Z9–14∶Ac,Z11–14∶Ac, and saturated 14∶Ac were found to be necessary for communication butE11–14∶Ac was found to have no effect on typical pheromone-mediated reproductive behaviors. Close-range studies suggested that chemicals of low volatility, released from moths, were important in eliciting copulation attempts. Field studies reinforced laboratory findings regarding the effectiveness of different lures and indicated thatHeliothis traps were the most effective for monitoring.
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  • 24
    ISSN: 1573-1561
    Keywords: Spodoptera exempta ; Lepidoptera ; Noctuidae ; sex pheromone ; (Z)-9-tetradecenal ; (Z)-9-tetradecenyl acetate ; (Z)-11-tetradecenyl acetate ; (Z,E)-9,12-tetradecadienyl acetate ; (Z)-9-tetradecen-1-ol ; (Z)-11-hexadecenyl acetate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Ovipositor washings from virgin femaleSpodoptera exempta (Walker) (Lepidoptera: Noctuidae) were analyzed by high-resolution gas chromatography (GC) linked to a male electroantennogram (EAG). GC retention times of the two major EAG responses observed were consistent with their assignment as (Z)-9-tetradecenyl acetate and (Z,E)-9,12-tetradecadienyl acetate, as previously identified. However, three other EAG responses were also noted that had GC retention times consistent with (Z)-9-tetradecenal, (Z)-9-tetradecen-1-o 1, and (Z)-11-hexadecenyl acetate. The components were present in the ratio of 100∶5∶1.5∶3.5∶4, respectively. Further analysis of the ovipositor washings by GC linked to a mass spectrometer (GC-MS) confirmed these findings and indicated the presence of a sixth component consistent with (Z)-11-tetradecenyl acetate present at 2 % of the major component. In field tests carried out in Kenya, (Z)-11-hexadecenyl acetate was the only newly identified component to enhance the catch of the original two-component mixture when presented in their natural ratio. The addition of (Z)-9-tetradecen-1-ol reduced catch, while (Z)-9-tetradecenal and (Z)-11-tetradecenyl acetate had no apparent effect.
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  • 25
    ISSN: 1573-1561
    Keywords: Campoletis sonorensis ; Hymenoptera ; Ichneumonidae ; Heliothis zea ; Lepidoptera ; Noctuidae ; 2-tridecanone ; trichomes ; Lycopersicon hirsutum f.glabratum ; tomato ; plant defense ; allelochemical
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Greenhouse-grown plants of five tomato lines varying in their level of 2-tridecanone-mediated resistance toManduca sexta (L.) andLeptinotarsa decemlineata (Say) were found to adversely affect larvae ofCampoletis sonorensis (Cameron), a larval endoparasitoid ofHeliothis zea (Boddie), in a manner directly related to their level of resistance. The parasitoid larvae, which emerge as fifth instars from their host and construct a cocoon on the foliage of their hosts' host plant, suffered extensive mortality during cocoon spinning on highly resistant foliage. Mortality was greatest (82%) on the highly resistant plants ofLycopersicon hirsutum f.glabratum (accession PI 134417) and an F1 backcross [(L. esculentum × PI 134417) × PI 134417] selection. Mortality was intermediate (40 and 28%, respectively) on backcross selections with moderate and low levels of resistance and least (8%) on susceptibleL. esculentum. Removal of the glandular trichomes, which contain 2-tridecanone in their tips, from the foliage eliminated differences in parasitoid mortality among plant lines. Bioassays of 2-tridecanone indicated that it is acutely toxic to fifth instarC. sonorensis larvae at the quantities associated with highly resistant foliage and produces symptoms identical to those observed on resistant foliage. 2-Undecanone, a second methyl ketone present in the glandular trichomes of resistant foliage, was also toxic toC. sonorensis larvae, but significantly less so than 2-tridecanone. The results support the hypothesis that 2-tridecanone is responsible for the observed mortality ofC. sonorensis larvae during cocoon construction on resistant foliage.
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  • 26
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    Journal of chemical ecology 15 (1989), S. 141-168 
    ISSN: 1573-1561
    Keywords: Hymenoptera ; Braconidae ; Microplitis croceipes ; Heliothis zea ; Lepidoptera ; Noctuidae ; anemotaxis ; parasitoid ; jet plumes ; optomotor response ; semiochemicals
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Odor-mediated host-searching by femaleMicroplitis croceipes, a braconid endoparasitoid of larvalHeliothis species, was studied in a flight tunnel. Volatiles emitted by third-instarH. zea feeding on cowpea seedling leaves were released and carried with the wind, resulting either in an irregularly shaped plume, or injected with high velocity, resulting in a conically shaped jet plume. Flight maneuvers ofM. croceipes had a higher stereotype in jet plumes compared to irregularly shaped plumes. Variation in odor concentration in jet plumes due to intermittent feeding of the host larvae did not affect the number of flights. At the levels employed, odor concentration did not alter the rate of upwind progress in jet plumes. Because air velocity inside a jet increases in the upwind direction,M. crocepies regulates its ground-speed during its approach to the odor source.
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  • 27
    ISSN: 1573-1561
    Keywords: Heliothis subflexa ; Lepidoptera ; Noctuidae ; attractant ; kairomone ; groundcherry ; Physalis angulatus ; flight tunnel bioassay ; plant-insect interaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Mated femaleHeliothis subflexa (Gn.) (HS) moths 1–7 days old responded positively in a Plexiglas flight tunnel to an attractant extracted with methanol from fresh whole-leaf washes of groundcherry,Physalis angulata L. Response to the groundcherry extract, as indicated by plume-tracking (i.e., upwind flight toward the odor source) and contact with the chemical dispenser did not change significantly during the first 5 hr of scotophase. Overall, ca. 50% of the responding moths also landed on the chemical dispenser; ca. 50% of the moths that landed also deposited eggs. There were no significant differences in the behavioral responses of females mating only once and those that had mated two or more times. Virgin females and male moths were significantly less responsive to the groundcherry attractant than mated females. The flight tunnel bioassay described provides an excellent system for evaluating plant allelochemics associated with host-plant selection.
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  • 28
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    Journal of chemical ecology 15 (1989), S. 265-273 
    ISSN: 1573-1561
    Keywords: Drosophila malerkotliana ; Diptera ; Drosophilidae ; aggregation pheromone ; (Z)-11-eicosenyl acetate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract (Z)-11-Eicosenyl acetate (Z11–20:Ac) was identified as the aggregation pheromone inDrosophila malerkotliana. The pheromone (200–300 ng/fly) was isolated from hexane extracts of the ejaculatory bulb of sexually mature male flies. Males released very little, if any,Z11–20:Ac to the food at any time. During mating there was a transfer of ca. 100 ng ofZ11–20:Ac to the female's reproductive tract. The mated female fly transferred theZ11–20:Ac to the surrounding surfaces in just a few hours after mating. In bioassay in a wind-tunnel olfactometer,Z11–20:Ac was not attractive alone, but was synergistic with fermenting food or with acetone. AlthoughD. malerkotliana has no (Z)-11-octadecenyl acetate (Z11–18:Ac), it was as attracted toZ11–18:Ac as to an equal quantity ofZ11–20:Ac.D. melanogaster andD. simulans, however, responded to theZ11–18: Ac that they produced and did not respond toZ11–20:Ac.
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  • 29
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    Journal of chemical ecology 15 (1989), S. 2101-2113 
    ISSN: 1573-1561
    Keywords: Pieris rapae ; Trichoplusia ni ; Lepidoptera ; Pieridae ; Noctuidae ; proteases ; digestive enzymes ; proteinase inhibitors ; dietary protein requirement
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract In their larval luminal midgut fluid,Trichoplusia ni (Lepidoptera: Noctuidae) andPieris rapae (Lepidoptera: Pieridae) contain endopeptidases as their primary proteases. Neither species has detectable exopeptidase activity. Studies using enzyme-specific substrates and inhibitors demonstrate that the endopeptidases are serine proteinases (both trypsinlike and chymotrypsinlike) with histidine at the active site. Optimal pH for the tryptic and chymotryptic activity is 8.5 and 8.0, respectively, forT. ni. and 8.0 and 9.0, respectively, forP. rapae. The efficiency of proteolytic digestion (as measured by the rate of in vitro digestion of a standard protein by the midgut luminal fluid) is positively correlated with the larval dietary protein requirement and is significantly influenced by the ratios of tryptic to chymotryptic activity present in the gut lumen of these two species of Lepidoptera.
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  • 30
    ISSN: 1573-1561
    Keywords: Ceratitis capitata ; medfly ; Diptera ; Tephritidae ; trimedlure ; electroantennogram ; dosage-response
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Electroantennograms (EAGs) of unmated laboratory-reared male and femaleCeratitis capitata (Wiedemann) were recorded in response to the attractant trimedlure [tert-butyl 4(and 5)-chloro-trans-2-methylcyclohexane-1-carboxylate] and its fourtrans isomers. For both sexes, the magnitude of the EAG response was relatively low as compared to other previously tested compounds (i.e., plant volatiles). Dosage-response curves generated for all TML isomers revealed that flies responded to increasing dosages over a relatively narrow range (two to three log steps). Responses for both sexes peaked at ca. 10 μg dose for all isomers. Antennal response in males was greatest to the C isomer followed by the B1, A, and B2 isomers, while responses of females were greatest for the A isomer followed by B1, C, and B2. Both sexes exhibited a long recovery period for the response potential to return to baseline at doses above 1 μg for all of the isomers tested, except for B2. The low EAG sensitivity to trimedlure and the apparent EAG selectivity to the C isomer in males are discussed in relation to the known field attractancy of males to the C, A, B1, and B2 isomers.
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  • 31
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    Journal of chemical ecology 15 (1989), S. 2233-2245 
    ISSN: 1573-1561
    Keywords: Sex pheromone ; Lepidoptera ; Noctuidae ; Heliothis peltigera ; capillary chromatography ; DMDS derivatization ; behavioral tests ; field work
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Ten compounds were found in the sex pheromone glands ofHeliothis peltigera (Schiff) and identified as tetradecenal, (Z)-9-tetradecenal, (Z)-9-tetradecenol, (Z)-9-tetradecenyl acetate, hexadecanal, (Z)-7-hiexadecenal, (Z)-9-hexadecenal, (Z)-11-hexadecenal, (Z)-11-hexadecenol, and (Z)-11-hexadecenyl acetate. Behavioral tests in a wind tunnel and subsequent trapping studies conducted in the field indicated that (Z)-11-hexadecenal and (Z)-9-tetradecenal are the main pheromone components ofH. peltigera. Addition of (Z)-11-hexadecenol to the binary blend did not enhance the capture of males ofH. peltigera, but it decreased the number of males of the sympatricH. armigera. Rubber septa impregnated with a mixture of 2 mg (Z)-11-hexadecenal + 1 mg (Z)-9-tetradecenal + 0.6 mg (Z)-11-hexadecenol are recommended for monitoringH. peltigera.
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  • 32
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    Journal of chemical ecology 15 (1989), S. 121-128 
    ISSN: 1573-1561
    Keywords: Spodoptera frugiperda ; Lepidoptera ; Noctuidae ; Meliaceae ; neem ; insecticides ; limonoids ; seed extracts
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Hexane and ethanol extracts of seeds from 10 plant species (including neem—Azadirachta indica A. Juss.) of the family Meliaceae were incorporated into artificial diet at various doses and fed to fall armyworm [Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae)] larvae in nochoice tests. All produced significant mortality, reduced larval growth rate, increased time to pupation, or all three, at some concentration. The two highest doses of all of the ethanol extracts caused 100% mortality before pupation, but the hexane extracts tended to be less effective.Aglaia cordata Hiern. ethanol extract was as potent as the comparable neem seed extract at virtually all levels, and its hexane extract was active at much lower concentrations than the neem extract was. The sublethal effects (slower growth and increased time to pupation) were usually detectable at lower doses of extract than mortality was.
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  • 33
    ISSN: 1573-1561
    Keywords: Pine beauty moth ; Panolis flammea ; Lepidoptera ; Noctuidae ; sex attractant ; monitoring trap ; chemoorientation ; odor plume ; trap interference
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Catches of malePanolis flammea in traps baited with lures containing 25 μg of sex attractant are enhanced when in the proximity of traps baited with 125-μg lures. The degree of enhancement is increased as the intertrap distance is decreased, and when the low-dose trap is upwind of the high-dose one. The patterns of alteration in trap catch suggest that moths initially attracted by one lure may divert into other traps, which may be either upwind of the original trap (“overshooting”), or downwind (“undershooting”). “Overshooting” can result in up to fivefold increases in catch and may provide a useful method for estimating the attractant zone of particular lure/trap combinations.
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  • 34
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    Journal of chemical ecology 15 (1989), S. 2625-2634 
    ISSN: 1573-1561
    Keywords: Pheromone ; disruption ; atmospheric permeation ; neurophysiology ; Heliothis zea (Boddie) ; Noctuidae ; Lepidoptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract For some species, chemical analogs have been identified that can substitute for the major pheromone component and reduce pheromone-trap captures in atmospheric permeation experiments. The ability to substitute these analogs for the major pheromone component in field tests raises the question: Do the same set of olfactory receptor neurons on the insects' antennae respond to both the major component and the effective structural analogs? To investigate this question, extracellular responses were recorded from single sensilla on the antennae of maleHeliothis zea (Boddie) (Lepidoptera: Noctuidae) to stimulation with increasing doses of (Z)-11-hexadecenal, the major pheromone component, (Z)-9-tetradecen-1-ol formate, and (Z)-1,12-heptadecadiene. Both (Z)-9-tetradecen-1-ol formate and (Z)-1,12-heptadecadiene can substitute for (Z)-11-hexadecenal in trap disruption experiments and affect the sexual behavior of males, although neither has been identified in the volatiles released by calling females. All three compounds elicited responses from the same class of pheromone-sensitive receptor neurons over a range of stimulus concentrations. At equivalent stimulus concentrations, however, (Z)-11-hexadecenal elicited about a 10-fold greater response than (Z)-1,12-heptadecadiene. (Z)-9-Tetradecen-1-ol formate elicited a response slightly higher than, but not significantly different from, the response to (Z)-1,12-heptadecadiene.
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  • 35
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    Journal of chemical ecology 15 (1989), S. 2577-2588 
    ISSN: 1573-1561
    Keywords: Drosophila busckii ; D. mulleri ; Diptera ; Drosophilidae ; aggregation ; pheromone ; (S)-2-pentadecyl acetate ; 2-pentadecanone ; chiral
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract (S)-2-Pentadecyl acetate and 2-pentadecanone were identified as the major aggregation pheromone components, inDrosophila busckii. Both sexes of flies were attracted equally in a wind-tunnel olfactometer. The flies also responded to racemic 2-pentadecyl acetate but not to the pureR enantiomer. In bioassay, (S)-2-pentadecyl acetate and 2-pentadecanone were each active alone, and a mixture of both increased the number of flies responding ca. twofold. The aggregation pheromone components are found in the ejaculatory bulb of sexually mature males and are transferred primarily to the female cuticle during mating. One third of the pheromone transferred is released by the female to the surrounding environment in a few hours after mating. None of the aggregation pheromone components remained on the mated female's cuticle, leaving two thirds unaccounted for. The same results were obtained when racemic 2-pentadecyl acetate was topically applied to immature and mature virgin males and females. BothD. mulleri andD. busckii were attracted to (S)-2-acetates of 13, 14 and 15 carbons, butD. mulleri preferred (S)-2-tridecyl acetate andD. busckii preferred (S)-2-pentadecyl acetate.
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  • 36
    ISSN: 1573-1561
    Keywords: Dacus dorsalis ; Oriental fruit fly ; Diptera ; Tephritidae ; methyl eugenol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Laboratory-reared and wild, fruit-reared adults of the Oriental fruit fly,Dacus dorsalis Hendel, were tested for response to methyl eugenol at various ages. Virgin laboratory (2, 4, 6, 8, and 10 days old) and wild (7, 11, 15, 19, and 23 days old) flies were released into an outdoor field cage and trapped over a two-day period. Response of males increased with age as ca. 32% and 22% of laboratory and wild males responded at 2 and 7 days of age, respectively, while ca. 93% of both strains responded at 10 and 23 days of age, respectively. These correspond approximately to the ages at which they reach sexual maturity. Female response did not increase with age and fluctuated between 15% and 29% for the laboratory strain and 10% and 45% for the wild strain. The age-related response profiles, when integrated with sexual maturation curves, indicate that one of the major reasons the male-annihilation technique is effective is because methyl eugenol is able to attract 40–50% of male flies prior to the onset of sexual maturation.
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  • 37
    ISSN: 1573-1561
    Keywords: Mating behavior ; Parasitoids ; Cotesia (=Apanteles)marginiventris ; Hymenoptera ; Braconidae ; Spodoptera frugiperda ; Lepidoptera ; Noctuidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Two blends of fall armyworm,Spodoptera frugiperda (J.E. Smith), pheromonal components were evaluated in the laboratory for possible close-range effects on mating and oviposition byCotesia marginiventris (Cresson). Neither a two- nor a five-component blend had any significant effect on mating or parasitization ofS. frugiperda larvae by this parasitoid. Dosages of 200, 400, and 800 μg were tested.
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  • 38
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    Journal of chemical ecology 15 (1989), S. 25-36 
    ISSN: 1573-1561
    Keywords: Screwworm fly ; Cochliomyia hominivorax ; Diptera ; Calliphoridae ; attractant ; host finding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract An olfactometer bioassay was used to follow attractant for screwworm flies,Cochliomyia hominivorax, in steam distillates of bovine blood under different distillation and storage conditions and after HPLC separation of components in a water-methanol gradient. In addition, fly responsiveness was examined in relation to sex and ovarian stage. Gravid and vitellogenic nullipars were attracted to the blood, although the former predominated four to one. Males did not respond at a dose that attracted 76% of gravid females. Maximum attractiveness occurred when distillate was stored in sealed glass ampoules. An argon atmosphere made storage at ambient temperatures feasible, but offered no advantage during storage at ca. −60°C or during distillation. The HPLC separation produced four fractions that duplicated the attractiveness of the distillate when recombined but showed little activity when presented as two-fraction, and most three-fraction, mixtures. Availability of the HPLC fractions for combination with other samples will facilitate location via bioassay of attractant components in samples obtained from subsequent or alternate isolations that preserve only one or two elements of the multicomponent mixture.
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  • 39
    ISSN: 1573-1561
    Keywords: CarrotDaucus carota ; Psila rosae ; Diptera ; Psilidae ; carrot-fly larva ; root chemicals ; carbonyl-rich fraction ; falcarinol ; falcarindiol ; fal-carindiol monoacetate ; electrophysiology ; neurotoxin ; carrot resistance factor
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Roots of the carrot cultivars Vertou L.D. (resistant) and Long Chantenay (susceptible) were subjected to detailed chemical analysis to identify extracts and compounds influencing larval host-finding (preference/non-preference) behavior and to compare concentrations of these compounds in resistant and susceptible cultivars. Vertou yielded threefold less volatile material in headspace extracts of pureed roots. Extracts of chopped root in methanol, steam, hexane, and chloroform were inactive in behavioral assays. However, ether extracts were active and their hydrocarbon and carbonyl-rich fractions contained potent attractants. The principal constituent of the carbonyl-rich fraction of each cultivar was the carotatoxin complex comprising the neurotoxin falcarinol (carotatoxin), falcarindiol, and falcarindiol monoacetate, the latter compound here reported for the first time from carrot. Falcarinol (50 and 100 μg) was active in a behavioral assay, and all three ingredients of the complex were potent electrophysiological stimuli, eliciting maximum single unit responses to source concentrations of 10 ng. Furthermore, the complex was more abundant by about 1000 μg/root in Long Chantenay. As this comprised extra amounts of 70, 862, and 110 μg of falcarinol, falcarindiol, and falcarindiol monoacetate, respectively, the observed differences seem both behaviorally and physiologically relevant. It is generally accepted that coevolution has transformed the role of many toxins into host-location cues, but this seems a relatively rare example of a neurotoxin (falcarinol) evincing, in decreased concentrations, nonpreference host resistance. This evolved response to a toxin present in large concentrations is contrasted with that totrans-2-nonenal, which paralyses and kills the larva and is present in only trace amounts in the root.
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  • 40
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    Journal of chemical ecology 15 (1989), S. 447-455 
    ISSN: 1573-1561
    Keywords: Hormonal control ; sex pheromone ; Heliothis armigera ; Spodoptera littoralis ; Lepidoptera ; Noctuidae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Heliothis armigera andSpodoptera littoralis females produced sex pheromone during their scotophase. Maximum levels of pheromone in Heliothisarmigera were extracted from 2- to 3-day-old females during their 4.5-hr scotophase. On ligation between the head and the thorax of both species, normal pheromone production was inhibited. Homogenates of brains (consisting of brains, subesophageal ganglia, corpora cardiaca, and corpora allata) from both species induced a normal level of pheromone production by ligated females. Moreover, inS. littoralis, female brain hormone induced the production of the two components (Z)-9- and (Z, E)-9,11-tetradecenyl acetate tested by gas chromatography. Partial separation of brain homogenates, using low pressure C18-silica reversed-phase columns, produced a stable and active hormone source that gave a dose-dependent response. This hormone was found in brain extracts of both male and femaleH. armigera andS. littoralis females. In addition,S. littoralis induced pheromone production byH. armigera ligated females.
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  • 41
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    Journal of chemical ecology 15 (1989), S. 929-938 
    ISSN: 1573-1561
    Keywords: Spodoptera littoralis ; Lepidoptera ; Noctuidae ; Egyptian cotton ; leaf worm ; oviposition behavior ; oviposition deterrence ; larval frass
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Previous experiments demonstrated an oviposition-deterring effect of larval frass in the Egyptian cotton leaf worm,Spodoptera littoralis (Boisd.). In this study, females were shown to perceive the oviposition-deterring substance(s) with their antennae. During dark, airtight, and cold (−10 °C) storage, the deterrent was persistent for at least 395 days. On the other hand, larval frass retained its activity for only two days when applied to cotton leaves. The deterrent activity of frass was independent of larval density. Frass of larvae reared at high densities deterred oviposition as well as frass of larvae feeding separately or in small groups. For significant oviposition deterrence, the minimum amount of frass was in the range of 5–10 mg frass per cotton leaf. An acetone extract of larval frass was highly deterrent, in contrast to extracts prepared with water, ethanol, chloroform, or pentane.
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  • 42
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    Journal of chemical ecology 15 (1989), S. 1867-1882 
    ISSN: 1573-1561
    Keywords: Carrot resistance ; carrot fly ; Psila rosae ; Diptera ; Psilidae ; main root factors ; nonpreference oviposition ; root resistance to larvae ; antibiosis ; root chemicals
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Field experiments on two different soil types in Ireland assessed the extent and mechanisms of resistance toPsila rosae (F.), the carrot fly, with emphasis on the role of the carrot root. Ten carrot cultivars gave consistent results in terms of resistant and susceptible cultivars. Nonpreference oviposition was confirmed as a mechanism, and the use of egg traps, providing differential exposure of the main root, showed this was regulated by root factors, probably chemical constituents. Independent main root resistance to the larva was also confirmed, and this effect was established as consistent with a chemically mediated nonpreference. Antibiosis by the root was demonstrated. Such effects in three different modes indicate that main root properties are crucial in carrot resistance toPsila and suggest a pervasive influence of root chemicals on such resistance.
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  • 43
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    Journal of chemical ecology 15 (1989), S. 1931-1946 
    ISSN: 1573-1561
    Keywords: Litchi chinensis ; Sapindaceae ; Ficus refusa ; Ficus benjamina ; Ficus benghalensis ; Moraceae ; Ceratitis capitata ; Diptera ; Tephritidae ; Mediterranean fruit fly ; medfly ; α-copaene ; α-ylangene
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Short-range attraction/feeding stimulation of male Mediterranean fruit flies [Ceratitis capitata (Wiedemann), (Diptera: Tephritidae)] to a stem extract of a host plant,Litchi chinensis Sonn. (Sapindaceae), and to milky exudates from stems of nonhost plants,Ficus retusa L. andF. benjamina L. (Moraceae), were attributed to the presence of the sesquiterpene α-copaene. The presence of α-copaene in the milky exudate from stems ofF. benghalensis L. is also suggested as eliciting similar behavioral responses in male medflies. The presence of minor quantities of α-ylangene in the plants and its contributory effects to the behavioral response of male medflies is discussed. Short-range attraction/feeding stimulation of male medflies to equal amounts of α-ylangene-free α-copaene samples (94.5%+), prepared from α-copaene-enriched angelica seed oil and copaiba oil, respectively, showed no difference in intensity of response. α-Ylangene elicited a slightly less intense response for male medflies than α-copaene.
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  • 44
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    Journal of chemical ecology 15 (1989), S. 399-411 
    ISSN: 1573-1561
    Keywords: Drosophila mulleri ; Diptera ; Drosophilidae ; aggregation ; pheromone ; chirality ; enantiomers ; ester ; ketone ; (S)-(+)-2-tridecanol acetate ; (Z)-10-heptadecen-2-one
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Existence of an aggregation pheromone was demonstrated inDrosophila mulleri. Mature males produce at least two compounds that are lacking from females and newly emerged males and that attract both males and females in a wind-tunnel bioassay. These compounds are (S)-(+)-2-tridecanol acetate and (Z)-10-heptadecen-2-one. Both were synthesized, and the flies responded to the synthetic compounds as well as to fly-derived preparations. The flies also responded to racemic 2-tridecanol acetate but not to the pureR enantiomer. A more polar, very volatile attractant was also extracted from both sexes ofD. mulleri but was not identified.
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  • 45
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    Journal of chemical ecology 15 (1989), S. 663-676 
    ISSN: 1573-1561
    Keywords: Drosophila ; Diptera ; Drosophilidae ; triterpene glycosides ; cactus ; fitness components ; host-plant relationships
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract The effects of pentacyclic triterpene glycosides extracted from agria and organ pipe cacti on three fitness parameters of the cactophilic fruit fly,Drosophila mojavensis were tested. Triterpene glycosides from organ pipe increased development time and reduced larval viability while those from agria produced smaller adults (reduced fecundity). In addition, the microbial communities in the organ pipe saponin media were less dense than those in the media to which agria saponins had been added. The role of cactus triterpene glycosides in the ecology of thisDrosophila species is discussed.
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  • 46
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    Journal of chemical ecology 15 (1989), S. 875-885 
    ISSN: 1573-1561
    Keywords: Hypericum perforatum ; phototoxicity ; hypericism ; Platynota flavedana ; Tortricidae ; Heliothis zea ; Noctuidae ; Lepidoptera
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Hypericin, an anthrone dimer inHypericum perforatum (Guttiferae), is shown for the first time to be phototoxic to generalist lepidopteran larvae; survivorship was reduced and development prolonged forHeliothis zea (Noctuidae) andPlatynota flavedana (Tortricidae) that consumed hypericin diets in the presence of light. Survivorship for both species is enhanced when photoactivating wavelengths are excluded by filters. In natureP. flavedana successfully survives onH. perforatum by tying together leaves and feeding inside the ties. Shielded from light, the larvae are protected by their mode of feeding from phototoxic compounds.
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  • 47
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    Journal of chemical ecology 15 (1989), S. 905-916 
    ISSN: 1573-1561
    Keywords: Onion fly ; Delia antiqua ; Diptera ; Anthomyiidae ; Erwinia carotovora varcarotovora ; Klebsiella pneumoniae ; food attractant ; ovipositional stimulant ; dipropyl disulfide ; 2-phenylethanol ; pentanoic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Decomposing onions at certain microbial successional stages produce potent volatile attractants and ovipositional stimulants of the onion fly,Delia antiqua (Diptera: Anthomyiidae). A reproducible source of these compounds was obtained by culturingErwinia carotovora var.carotovora (EC) on sterile onion tissue. In laboratory choice tests, EC-inoculated onion was more attractive thanKlebsiella pneumoniae (KP) cultured on onion, EC cultured on potato (a nonhost of onion fly), or the chemical synthetic baits dipropyl disulfide and an aqueous solution of 2-phenylethanol and pentanoic acid. Onion flies were mildly attracted to potato after inoculation with EC, but females did not accept EC-inoculated potato for oviposition. This work emphasizes that sources of semiochemicals may need to be defined microbiologically as well as physically and chemically.
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  • 48
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    Journal of chemical ecology 15 (1989), S. 1241-1247 
    ISSN: 1573-1561
    Keywords: (Z)-11-Hexadecenyl acetate ; dodecyl acetate ; (Z)-11-hexadecenal ; (Z)-11-hexadecenol ; Sesamia nonagrioides ; Lepidoptera ; Noctuidae ; sex pheromone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Z-11-Hexadecenyl acetate (Z11–16∶OAc), dodecyl acetate (12∶OAc),Z-11-hexadecenal (Z11–16∶Aid), andZ-11-hexadecenol (Z11– 16∶OH), were found in pheromone gland extracts of femaleSesamia nonagriodes (Lef.) [Lepidoptera: Noctuidae]. These four components were also present in airborne volatiles collected from calling virgin females in a 65∶18∶8∶9 ratio. Hexadecyl acetate (16∶OAc) was also detected but found to be inactive. The identification was based on multicolumn GC analysis, mass spectrometry, and field activity.Z11–16∶OAc is the major sex pheromone component; the addition of the secondary components individually decreased male captures. The blend of the four synthetic components in 69∶15∶8∶8 ratio was highly attractive to males; 200 μg per trap was the most effective concentration in field tests.
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  • 49
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    Journal of chemical ecology 15 (1989), S. 1379-1389 
    ISSN: 1573-1561
    Keywords: Artemisia tridentata ; Chamomilla suaveolens ; Chrysothamnus nauseosus ; Peridroma saucia ; Lepidoptera ; Noctuidae ; growth inhibition ; plant extracts ; pest control
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Petrol and ethanolic extracts of six asteraceous weeds were added to artificial diets to screen for growth inhibition and mortality of the variegated cutworm,Peridroma saucia (Hbn). Petrol and ethanolic extracts ofArtemisia tridentata andChamomilla suaveolens and ethanolic extracts ofChrysothamnus nauseosus andCentaurea diffusa severely inhibited larval growth at five times the natural concentrations. The twoC. suaveolens extracts and the ethanol extract ofA. tridentata were active at the natural concentration (100%) and were further examined at 20, 40, 60, and 80% of this level. Inhibition of larval growth was directly related to concentration for each of the three extracts tested. EC50s (effective concentration to inhibit growth by 50% relative to controls) for the three extracts were 36–42% of the naturally occurring level in the plants. Nutritional indices were calculated for secondinstarP. saucia feeding on the activeA. tridentata EtOH extract and the petrol extract fromC. suaveolens. Addition of the activeA. tridentata EtOH or theC. suaveolens petrol extract to the diet resulted in significant reduction in the relative growth rate of larvae, although theA. tridentata extract was much more inhibitory. Dietary utilization was significantly lower for larvae fed theA. tridentata EtOH extract.
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  • 50
    ISSN: 1573-1561
    Keywords: Campoletis sonorensis ; Hymenoptera ; Ichneumonidae ; Heliothis zea ; Lepidoptera ; Noctuidae ; trichome ; 2-tridecanone ; allelochemical ; plant resistance ; Lycopersicon hirsutum f.glabratum ; tomato
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Chemistry and Pharmacology
    Notes: Abstract Field populations ofHeliothis spp. were sampled for levels of naturally occurring larval parasitism on six tomato lines varying in levels of 2-tridecanone-mediated resistance toManduca sexta (L.) andLeptinotarsa decemlineata (Say). Second and third instars were parasitized byCampoletis sonorensis (Cameron) (Hymenoptera: Ichneumonidae) andCotesia (=Apantales)marginiventris (Cresson) (Hymenoptera: Braconidae) in 1984 through 1986 and byMicropletis croceipes (Cresson) (Hymenoptera: Braconidae) in 1986. Differences in parasitism by individual and multiple species among host plants were not demonstrated. However, levels of parasitism were low and variable among replicates. Total larval parasitism averaged across all plant lines was less than 6% in 1984 and 1986 and approximately 11% in 1985. In laboratory cage studies,C. sonorensis parasitized fewerH. zea larvae on tomato foliage with high levels of 2-tridecanone than on foliage with low levels. RearingH. zea on diet containing 2-tridecanone and 2-undecanone did not alter incidence of parasitism byC, sonorensis; nor did rearing parasitizedH. zea larvae on chemically treated host diets precondition the parasitoid to higher or lower mortality when transferred to foliage as a substrate for cocoon spinning, regardless of the foliage genotype. However, parasitoid survival during cocoon spinning on foliage varied significantly among plant lines in a manner corresponding to the level of 2-tridecanone-mediated resistance of the foliage. Parasitoid mortality was greatest on highly resistant foliage and lowest on susceptible foliage.
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