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  • Drosophila  (266)
  • Triticum aestivum  (263)
  • Springer  (529)
  • Cell Press
  • 1985-1989  (308)
  • 1980-1984  (221)
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  • 1
    ISSN: 1432-0789
    Schlagwort(e): Wheat ; Paddy straw compost ; N and P enrichment ; Rock phosphate ; Pyrite ; Triticum aestivum ; Nutrient uptake
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Geologie und Paläontologie , Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary A nutrient-rich compost from paddy straw was prepared using urea and Mussoorie rock phosphate for N and P enrichment respectively. Inorganic N was partly conserved in the compost by the addition of pyrite. Citric-acid-soluble P also increased with the addition of pyrite. Compost containing about 1.6% total N and 3.3% total P was found to be a good source of P for a wheat crop and also supplied a significant amount of N to the plants.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Journal of molecular evolution 28 (1989), S. 220-224 
    ISSN: 1432-1432
    Schlagwort(e): Drosophila ; Foldback element ; Transposable element
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Foldback elements are a family of transposable elements described inDrosophila melanogaster. The members of this dispersed repetitive family have terminal inverted repeats that sometimes flank a central region. The inverted repeats of all the family members are homologous. The study of the distribution and conservation of the foldback elements in differentDrosophila species shows that this distribution is different from that of the hybrid dysgenesis systems (PM and IR). Sequences homologous to foldback elements were observed by Southern blots and in situ hybridization in all species of themelanogaster subgroup and in some species of themontium andtakahashii subgroups. The element was probably already present before the radiation of these subgroups. No evidence of horizontal transmission of the foldback element could be observed.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    Springer
    Nutrient cycling in agroecosystems 20 (1989), S. 59-66 
    ISSN: 1573-0867
    Schlagwort(e): Ammonia volatilization ; nitrogen leaching ; denitrification ; time of N application ; wheat ; Triticum aestivum ; Triticale ; irrigation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Grain yield, nitrogen (N) assimilation, ammonia (NH3) volatilization, denitrification and fertilizer N distribution were examined in three commercially grown cereal crops; two were sown into conventionally tilled fields, while the third was direct drilled into an untilled field. The crops were top dressed with urea at establishment, tillering or ear initiation. Crop yield and N assimilation were measured in 16 m by 2.5 m plots receiving 0, 35, 70, 105, 140 or 175 kg N ha−1. A mass balance micrometeorological technique was used to measure NH3 volatilization, and other fertilizer N transformations and transfers were studied using15N labelled urea in microplots. On the conventionally tilled sites application of urea increased the grain yield of wheat from 3.9 to 5.5 t ha−1, when averaged over the five application rates, three application times and two sites. There were no site or application time effects. However, on the direct drilled site, time of application had a significant effect on grain yield. When urea was applied at establishment, grain yield was not significantly increased and the mean yield (2.81 t ha−1) was less than that obtained from treatments fertilized at tillering or ear initiation (4.09 and 4.0 t ha−1, respectively). Much of the variation in grain yield at the no-till site could be ascribed to differences in NH3 volatilization. At the no-till site, NH3 losses were equivalent to 24, 12 and 1% of the N applied at establishment, tillering and ear initiation, respectively. Negligible volatilization of NH3 occurred at the other sites. The surface soil at the no-till site had the highest urease activity and the soil was covered with alkaline ash resulting from stubble burning. Plant recovery of fertilizer N did not vary with application time on conventionally tilled sites (mean 62%). However, plant recovery of15N applied to the no-till site at establishment (35% of the applied N) was significantly less than that from plots where the application was delayed (45% at tillering and 55% at ear initiation, respectively). Leaching of N to below 300 mm depth was minimal (0 to 5% of the applied N). The calculated denitrification losses ranged from 1% to 14% of the applied N. The results show that the relative importance of NH3 volatilization, leaching and denitrification varied with site and fertilization time. The importance of the various N loss mechanisms needs to be taken into account when N fertilization strategies are being developed.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 4
    Digitale Medien
    Digitale Medien
    Springer
    Development genes and evolution 198 (1989), S. 227-232 
    ISSN: 1432-041X
    Schlagwort(e): Campaniform sensilla ; Drosophila ; Achaete-scute complex ; Cis regulatory sites
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary We have analysed the role of the achaete-scute gene complex in the development of the pattern of campaniform sensilla on the wing blade of Drosophila. We show that the complete pattern results from the superimposition of two independent subpatterns, one of which depends on the achaete gene and the other on scute. The scute subpattern comprises several clusters of sensilla, most of which seem to require the presence of control regions located upstream of the transcribed region. This is in contrast with the pattern of scute-dependent bristles, most of which depends on control elements located downstream of the transcribed region.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 5
    Digitale Medien
    Digitale Medien
    Springer
    Development genes and evolution 198 (1989), S. 65-77 
    ISSN: 1432-041X
    Schlagwort(e): Cell communication ; Pattern formation ; Cell differentiation ; trans-regulatory genes ; Drosophila
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The effects ofpolyhomeotic (ph) mutants in imaginal cells have been studied in a clonal analysis. Clones of cells, homozygous forph, sort-out after a few divisions, probably as a consequence of modified cell affinities. The dorso-ventral margin of the wing has special characteristics that retard this phenomenon. The formation and exclusion of a clone of 8–16 cells affect the polarity of the wild-type neighbour cells and can provoke pattern triplications. The results suggest that a defect in intercellular communication prevents the wild-type cells from maintaining coordinated positional information. The cells react by regenerative growth, and reorganize into a new pattern. The pleiotropic phenotypes ofph mutants are explained according to a common hypothesis aboutph + function.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 6
    Digitale Medien
    Digitale Medien
    Springer
    Development genes and evolution 198 (1989), S. 157-169 
    ISSN: 1432-041X
    Schlagwort(e): Distal less ; Limb development ; Drosophila
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The development of all of the adult limbs inDrosophila depends upon the activity of theDistal-less gene. We report here the phenotypic characterization of a number of hypomorphicDistal-less alleles which indicates that there is a graded requirement forDistal-less activity in the developing limbs. Previous analysis of genetically mosaic animals indicated that cells in the early primordia of the limb imaginal dises possess a graded proximal-distal positional information which depends on the presence of theDistal-less gene for its expression. Taken together these data suggest thatDistal-less may directly encode the graded positional information that is required to organise the proximal-distal axis of the developing limbs.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 7
    Digitale Medien
    Digitale Medien
    Springer
    Development genes and evolution 198 (1989), S. 185-190 
    ISSN: 1432-041X
    Schlagwort(e): Drosophila ; Oogenesis ; Follicle cells ; Egg shell ; Ovarian tumor
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The developmental potential of the cells of the somatic follicular epithelium (follicle cells) was studied in mutants in which the differentiation of the germ-line cells is blocked at different stages of oogenesis. In two mutants, sn 36a and kelch, nurse cell regression does not occur, yet the follicle cells around the small oocyte continue their normal developmental program and produce an egg shell with micropylar cone and often deformed operculum and respiratory appendages. Neither the influx of nurse cell cytoplasm into the oocyte nor the few follicle cells covering the nurse cells are apparently required for the formation of the egg shell. In the tumor mutant benign gonial cell neoplasm (bgcn) the follicle cells can also differentiate to some extent although the germ-line cells remain morphologically undifferentiated. Vitelline membrane material was synthesized by the follicle cells in some bgcn chambers and in rare cases a columnar epithelium, which resembled morphologically that of wild-type stage-9 follicles, formed around the follicle's posterior end. The normal polarity of the follicular epithelium that is characteristic for mid-vitellogenic stages may, therefore, be established in the absence of morphologically differentiating germ-line cells. However, the tumorous germ-line cells do not constitute a homogeneous cell population since in about 30% of the analyzed follicles a cell cluster at or near the posterior pole can be identified by virtue of its high number of concanavalin A binding sites. This molecular marker reveals an anteroposterior polarity of the tumorous chambers. In follicles mutant for both bgcn and the polarity gene dicephalic the cluster of concanavalin A-stained germ-line cells shifts to more anterior positions in the follicle.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 8
    ISSN: 1432-1939
    Schlagwort(e): Drosophila ; Microbial associations ; Resource partitioning
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The distributions of five Drosophila species and four components of the microflora have been compared across a total of 48 traps baited with four different fruit and vegetable substrates in two domestic compost heaps in Canberra (Australia). Large and consistent differences are found, both among the Drosophila and among the microbial classes, in their distributions across traps baited with different substrates. Moreover the distribution of each Drosophila species shows a unique set of strong associations with the microbial distributions. Thus the distributions of both D. simulans and D. melanogaster are found to be strongly negatively correlated with the abundance of bacteria while D. simulans is also strongly positively correlated with the titre of fermenter yeasts. D. immigrans is strongly positively correlated both with bacteria and with non-fermenter yeasts. D. hydei is positively correlated with nonfermentery yeasts and D. busckii is negatively correlated with fermenter yeasts. Moulds are the only microbial class not consistently associated with the distribution of any of the Drosophila species. The correlations with the other microbial classes are sufficient to explain the majority of the abundance differences of the Drosophila species among the trap types. It is therefore proposed that the clear partitioning of the fruit resources by the Drosophila is due to their differing primary interactions with the microflora.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 9
    ISSN: 1432-2145
    Schlagwort(e): Pollen development ; Triticum aestivum ; RH0007
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary A study of pollen development in wheat was made using transmission electron microscopy (TEM). Microspores contain undifferentiated plastids and mitochondria that are dividing. Vacuolation occurs, probably due to the coalescence of small vacuoles budded off the endoplasmic reticulum (ER). As the pollen grain is formed and matures, the ER becomes distended with deposits of granular storage material. Mitochondria proliferate and become filled with cristae. Similarly, plastids divide and accumulate starch. The exine wall is deposited at a rapid rate throughout development, and the precursors appear to be synthesized in the tapetum. Tapetal cells become binucleate during the meiosis stage, and Ubisch bodies form on the plasma membrane surface that faces the locule. Tapetal plastids become surrounded by an electron-translucent halo. Rough ER is associated with the halo around the plastids and with the plasma membrane. We hypothesize that the sporopollenin precursors for both the Ubisch bodies and exine pollen wall are synthesized in the tapetal plastids and are transported to the tapetal cell surface via the ER. The microspore plastids appear to be involved in activities other than precursor synthesis: plastid proliferation in young microspores, and starch synthesis later in development. Plants treated with the chemical hybridizing agent RH0007 show a pattern of development similar to that shown by untreated control plants through the meiosis stage. In the young microspore stage the exine wall is deposited irregularly and is thinner than that of control plants. In many cases the microspores are seen to have wavy contours. With the onset of vacuolation, microspores become plasmolyzed and abort. The tapetal cells in RH0007-treated locules divide normally through the meiosis stage. Less sporopollenin is deposited in the Ubisch bodies, and the pattern is less regular than that of the control. In many cases, the tapetal cells expand into the locule. At the base of one of the locules treated with a dosage of RH0007 that causes 95% male sterility, several microspores survived and developed into pollen grains that were sterile. The conditions at the base of the locule may have reduced the osmotic stress on the microspores, allowing them to survive. Preliminary work showed that the extractable quantity of carotenoids in RHOOO7-treated anthers was slightly greater than in controls. We concluded that RH0007 appears to interfere with the polymerization of carotenoid precursors into the exine wall and Ubisch bodies, rather than interfering with the synthesis of the precursors.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 10
    Digitale Medien
    Digitale Medien
    Springer
    Journal of comparative physiology 166 (1989), S. 179-187 
    ISSN: 1432-1351
    Schlagwort(e): Blowfly ; Drosophila ; Photoreceptor ; Lanthanum ; trp mutant
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary The effect of lanthanum on the light response of blowfly (Calliphora erythrocephala) photoreceptors was studied. The electrophysiological behaviour of the photoreceptors in the presence of La can be summarized as follows: 1. Upon long stimulation the photoreceptors responded with a ‘transient receptor potential’, i.e. the cells depolarized at the onset of the stimulus and then repolarized to (or below) the resting potential. This effect was dependent on stimulus intensity and occurred only at high intensities. During illumination membrane noise was reduced. 2. The light-induced changes in membrane potential were paralleled by changes in membrane resistance. 3. The time course of the receptor response was slowed down. 4. Light adaptation led to an increase in response latency. 5. The recovery of the receptor response after light adaptation was slowed down. 6. The sensitivity of the receptor cells measured by the response to short light stimuli was reduced. In summary, the electrophysiological behaviour of Calliphora photoreceptors in the presence of La was very similar to that of the photoreceptors of the trp (transient receptor potential) mutant of Drosophila melanogaster. This result suggests that La and trp mutation affect the same cellular processes in the photoreceptors.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 11
    Digitale Medien
    Digitale Medien
    Springer
    Ecological research 4 (1989), S. 209-218 
    ISSN: 1440-1703
    Schlagwort(e): Annual life cycle ; Drosophila ; Fungus preference ; Nematode parasitism
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Food preferences and nematode parasitism were studied in natural populations of mycophagousDrosophila in and near Sapporo, northern Japan. Species which preferred fresh mushrooms showed species-specific responses toPleurotus mushrooms:D. pirka bred only onPleurotus cornucopiae, D. trivitata onP. cornucopiae andP. ostreatus, D. trilineata on these twoPleurotus mushrooms and some other mushrooms, whileD. sexvittata bred on a wide variety of mushrooms but seldom onPleurotus mushrooms. Species which preferred decayed mushrooms (D. quadrivittata, D. histrioides, D. testacea and species of thequinaria species-group) showed host preferences different from those of the above species. The rate of parasitism by nematodes was generally higher in species which prefer decayed mushrooms than in species which prefer fresh mushrooms. Among species which prefer fresh mushrooms, onlyD. trilineata was parasitized frequently by nematodes. It was not clear what factors determine the rate of parasitism in these mycophagousDrosophila. D. pirka, D. trivittata andD. trilineata passed through three or four generations per year and entered reproductive diapause in early September in and near Sapporo.
    Materialart: Digitale Medien
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  • 12
    Digitale Medien
    Digitale Medien
    Springer
    Molecular and cellular biochemistry 90 (1989), S. 27-35 
    ISSN: 1573-4919
    Schlagwort(e): zerknullt gene ; homeobox protein ; Drosophila ; filter binding
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Summary The region upstream from the zerknullt (zen) gene contains three sites that specifically bind the zen protein product of the gene. Evidence for these binding sites was obtained by the filter binding technique and the DNase footprinting technique. The filter binding technique was used to scan various segments of DNA for the presence of possible specific binding sites. Segments that were selectively retained by the filter binding technique invariably contained one or more specific binding sites according to the DNase footprinting technique. Two of the zen protein binding sites were spaced only 30 base pairs apart. These sites could be separated without any loss in their specific binding properties. It is concluded that these two sites function independently in the binding of zen protein.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 13
    Digitale Medien
    Digitale Medien
    Springer
    Biochemical genetics 27 (1989), S. 507-520 
    ISSN: 1573-4927
    Schlagwort(e): Drosophila ; sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) ; glue proteins ; glycosylation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract The patterns of protein fractions from total salivary glands and from glue plugs were compared in seven members of theDrosophila nasuta subgroup by the use of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The glue protein patterns are member specific concerning the numbers and the electrophoretic mobilities of major and minor glue protein fractions. However, the major fractions of all subgroup members could be grouped into five SDS-PAGE domains according to the homologies of their electrophoretic mobilities, prominence of Coomassie blue staining, and PAS reaction. In all subgroup members, major fractions are involved in posttranslational modifications into larger protein molecules of the final glue. Quantitative estimations of the glue proteins inD. n. nasuta andD. n. albomicans reveal that they constitute between 55 and 60% of the total salivary gland proteins, whereas inD. melanogaster and inD. hydei the fraction is only 32 and 35%, respectively.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 14
    Digitale Medien
    Digitale Medien
    Springer
    Biochemical genetics 27 (1989), S. 507-520 
    ISSN: 1573-4927
    Schlagwort(e): Drosophila ; sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) ; glue proteins ; glycosylation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract The patterns of protein fractions from total salivary glands and from glue plugs were compared in seven members of theDrosophila nasuta subgroup by the use of sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The glue protein patterns are member specific concerning the numbers and the electrophoretic mobilities of major and minor glue protein fractions. However, the major fractions of all subgroup members could be grouped into five SDS-PAGE domains according to the homologies of their electrophoretic mobilities, prominence of Coomassie blue staining, and PAS reaction. In all subgroup members, major fractions are involved in posttranslational modifications into larger protein molecules of the final glue. Quantitative estimations of the glue proteins inD. n. nasuta andD. n. albomicans reveal that they constitute between 55 and 60% of the total salivary gland proteins, whereas inD. melanogaster and inD. hydei the fraction is only 32 and 35%, respectively.
    Materialart: Digitale Medien
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  • 15
    Digitale Medien
    Digitale Medien
    Springer
    Biochemical genetics 27 (1989), S. 263-277 
    ISSN: 1573-4927
    Schlagwort(e): Drosophila ; kinetic plate reader ; enzyme polymorphism
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Techniques for performing numerous enzyme kinetic assays with minimum time and effort would be valuable to studies of the evolutionary genetics of metabolic control and the quantitative genetics of determinants of kinetic parameters. Microtiter plate readers have been used for a variety of repetitious analytical techniques, and instruments are available that can take repetitive readings with sufficient speed to perform kinetic assays. The ability of these instruments to assay rapidly the kinetic properties of small samples makes them potentially useful for a number of problems in population genetics. While the ability to handle large numbers of samples is very attractive, the small sample volumes and optical imprecision of microtiter plates result in some sacrifice in accuracy. This paper presents methods for performing kinetic assays on individual field-caughtDrosophila, quantifies the precision of these methods, and characterizes differences amongDrosophila melanogaster andD. simulans from samples caught in California and Pennsylvania. Comparisons between field-caught and laboratory rearedD. melanogaster show that most of the characters are very similar, with the exception of αGPDH, which has a threefold higher mean activity among field-caught flies. The phenotypic correlations are presented with a brief discussion of their relevance to assessing the evolution of metabolic control of these enzymes.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 16
    Digitale Medien
    Digitale Medien
    Springer
    Biochemical genetics 27 (1989), S. 99-118 
    ISSN: 1573-4927
    Schlagwort(e): Drosophila ; aldox-2 ; molybdoenzymes ; sulfite oxidase ; molybdenum ; tungsten
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Mutation at thealdox-2 locus inDrosophila melanogaster affects the specific activities of four molybdoenzymes differentially during development. Sulfite oxidase activity is normal during late larval and pupal stages but is reduced during early adult stages inaldox-2 organisms. There was complete concordance among the effects ofaldox-2 on sulfite oxidase, aldehyde oxidase, xanthine dehydrogenase, and pyridoxal oxidase, when 38 stocks were analyzed which were derived from single recombination events betweenc andpx, markers which flankaldox-2. Several different biochemical analyses indicate that the active molybdoenzymes present in thealdox-2 strain are normal with respect to size, shape,pH-activity profile,K m , and molecular weight. Significant differences were found between thealdox-2 strain and the OR control strain in their responses to dietary Na2MoO4 and Na2WO4. The mutant strain is much more resistant to the effects of dietary Na2WO4 and much more responsive to the administration of Na2MoO4 than the OR control strain when these effects are quantitated by measurements of molybdoenzyme specific activities. This evidence suggests that thealdox-2 + gene product has a molybdenum binding site which can also bind tungsten and that this site is altered in the mutant strain. The hypothesis presented explains the observed effects of thealdox-2 mutation and relates them to the other mutations reported in this gene-enzyme system.
    Materialart: Digitale Medien
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  • 17
    ISSN: 1573-3297
    Schlagwort(e): Drosophila ; circadian clock ; ultradian oscillations ; disconnected mutant ; visual system
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Psychologie
    Notizen: Abstract Free-running locomotor activity and eclosion rhythms ofDrosophila melanogaster, mutant at thedisconnected (disco) locus, are substantially different from the wild-type phenotype. Initial periodogram analysis revealed little or no rhythmicity (Dushayet al., 1989). We have reanalyzed the locomotor activity data using high-resolution signal analysis (maximum-entropy spectral analysis, or MESA). These analyses, corroborated by autocorrelograms, uncovered significant residual circadian rhythmicity and strong ultradian rhythms in most of the animals tested. In this regard thedisco mutants are much like flies expressing mutant alleles of theperiod gene, as well as wild-type flies reared throughout life in constant darkness. We hypothesize that light normally triggers the coupling of multiple ultradian oscillators into a functional circadian clock and that this process is disrupted indisco flies as a result of the neural lesion.
    Materialart: Digitale Medien
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  • 18
    Digitale Medien
    Digitale Medien
    Springer
    Theoretical and applied genetics 77 (1989), S. 123-127 
    ISSN: 1432-2242
    Schlagwort(e): Inbreeding depression ; Drosophila ; Natural selection
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary This experiment was designed to study the relationship between rate of inbreeding and observed inbreeding depression of larval viability, adult fecundity and cold shock mortality in Drosophila melanogaster. Rates of inbreeding used were full-sib mating and closed lines of N=4 and N=20. Eight generations of mating in the N=20 lines, three generations in the N=4 lines and one generation of full-sib mating were synchronised to simultaneously produce individuals with an expected level of inbreeding coefficient (F) of approximately 0.25. Inbreeding depression for the three traits was significant at F=0.25. N=20 lines showed significantly less inbreeding depression than full-sib mated lines for larval viability at approximately the same level of F. A similar trend was observed for fecundity. No effect of rate of inbreeding depression was found for cold shock mortality, but this trait was measured with less precision than the other two. Natural selection acting on loci influencing larval viability and fecundity during the process of inbreeding could explain these results. Selection is expected to be more effective with slow rates of inbreeding because there are more generations and greater opportunity for selection to act before F=0.25 is reached. Selection intensities seem to have been different in the three traits measured. Selection was most intense for larval viability, less intense for fecundity and, perhaps, negligible at loci influencing cold shock mortality.
    Materialart: Digitale Medien
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  • 19
    Digitale Medien
    Digitale Medien
    Springer
    Theoretical and applied genetics 78 (1989), S. 728-734 
    ISSN: 1432-2242
    Schlagwort(e): Esterases ; Genetic variability ; Triticum aestivum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Genetic variability of endosperm esterase has been studied in 42 cultivars of Triticum aestivum L. 2n=6x=42. Different techniques, including sequential electrophoresis and electrofocusing, have been used with various substrates and esterase inhibitors. The electrophoretic patterns in each cultivar are described. Chromosomal location using the nullitetrasomic and ditelosomic lines of Chinese Spring was carried out in order to relate and/or locate the esterase genes to specific chromosomes. Most of the esterase isozymes located were in the long arm of the chromosomes of the homoeology group 3; but we have found six located in the short arms, five of them in the chromosome 3AS and one in the 3DS. This location increases the number of esterase genes described, because no esterase genes had been described so far in short arms of chromosomes of the homoeology group 3. The genetic control is discussed and, according to our results, between 12 and 15 loci, organized in five “compound loci”, control the endosperm esterases in wheat. Also one “modifier” gene modifying the mobility of two esterase bands and present in all the cultivars studied is postulated.
    Materialart: Digitale Medien
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  • 20
    Digitale Medien
    Digitale Medien
    Springer
    Theoretical and applied genetics 78 (1989), S. 873-878 
    ISSN: 1432-2242
    Schlagwort(e): Heat shock proteins ; 2D electrophoresis ; Triticum aestivum ; Gene location ; Gene regulation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The low molecular weight heat shock protein (HSP) profiles of the hexaploid wheat cultivar “Chinese Spring” and its ditelosomic series were characterized by isoelectric focusing polyacrylamide gel electrophoresis of denatured in vivo radiolabeled proteins. Comparisons of the ditelosomics (DTs) to the euploid “Chinese Spring” enabled the assignment of genes controlling 9 of the 13 targeted HSPs to seven chromosome arms. There did not appear to be a genome-specific action in the regulation of expression of these HSPs. There did appear to be a higher frequency of controlling genes within homoeologous DT lines 3, 4 and 7. Significant variation in protein quantity was evident among the DT lines for some HSPs, while other HSPs were remarkably stable in their expression across all DTs examined. The results are useful in identifying specific DT lines for the investigation of HSP functions in hexaploid wheat.
    Materialart: Digitale Medien
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  • 21
    Digitale Medien
    Digitale Medien
    Springer
    Theoretical and applied genetics 77 (1989), S. 162-168 
    ISSN: 1432-2242
    Schlagwort(e): Triticum aestivum ; Agropyron ; (Elytrigia, Thinopyrum) ; Intergeneric hybridization ; Crossability ; Wide crosses
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Intergeneric hybrids between Triticum aestivum L. and conventional rhizomatous Agropyron species were produced in variable frequencies. They were recovered in high percentage frequencies for T. aestivum cultivars with A. acutum (14.6%), A. intermedium (48.0%), A. pulcherrimum (53.3%), and A. trichophorum (46.6%). The crossability percentages with the highly crossable cultivar ‘Chinese Spring’ for these Agropyron species accessions were 33.12%, 65.0%, 53.3%, and 65.4%, respectively. Autosyndetic associations of two of their three genomes gave mean meiotic chromosome association data of 17.0 I (univalents) +1.53 II (ring bivalents) + 7.04 II (rod bivalents) +1.43 III (trivalents) +0.05 IV (quadrivalents) +0.01 IV (pentavalents) for A. acutum and of 21.8 I + 1.56 II (rings) +7.22 II (rods) +0.84 III + 0.04 IV for A. intermedium. Chromosome pairing at metaphase I was comparatively lower for A. pulcherrimum (34.4 I + 0.2 II (rings) +3.4 II (rods) +0.14 III) and A. trichophorum (36.7 I + 0.35 II (rings) +2.26 II (rods) + 0.04 III) hybrids with T. aestivum. Hybrids of wheat with A. campestre and A. repens were obtained in low frequency. Direct crossing did not permit T. aestivum/ A. desertorum hybridization. However, by utilizing the 2n=10x=70 A. repens/A. desertorum amphiploid as the pollen source, hybridization with T. aestivum did indeed occur. Aneuploidy was prevalent in this hybrid combination while all other hybrid combinations were apparently normal.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 22
    Digitale Medien
    Digitale Medien
    Springer
    Theoretical and applied genetics 77 (1989), S. 253-259 
    ISSN: 1432-2242
    Schlagwort(e): Heterosis ; Selection ; Drosophila ; Genotype x environment
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Experiments were designed to examine whether heterosis would occur in crosses of Drosophila melanogaster populations adapted to 18 °C or 28 °C environments. Crosses were examined in parental environments, an intermediate environment (23 °C) and a mixed environment (alternating 18°/28°C). Parental populations did not show divergence for larval viability, cold shock or high temperature mortalities when tested in a common environment. However, the 28 °C population was less fecund than the 18 °C population, but had higher larval competitive ability and higher adult longevity. Heterosis for viability, cold shock mortality and high temperature mortality occurred in crosses between a population adapted to 18 °C and another adapted to 28 °C, but not in crosses between two populations adapted to the same temperature. The results suggest that, in the absence of drift, heterosis is expected in crosses between lines or populations with different histories of selection but not between lines with the same selection histories.
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    Standort Signatur Erwartet Verfügbarkeit
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  • 23
    Digitale Medien
    Digitale Medien
    Springer
    Theoretical and applied genetics 78 (1989), S. 229-232 
    ISSN: 1432-2242
    Schlagwort(e): Triticum aestivum ; Puccinia recondita ; Adult plant resistance ; Resistance genes ; Durable resistance
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Wheat genotypes, including backcross derivatives of ‘Thatcher’ carrying Lr10 and Lr23, substitution lines for Lr10 and Lr23 in Chinese Spring background and Chinese Spring and Thatcher were analysed against 21 pathotypes of leaf rust in seedling tests. Adult plant responses in all these stocks were observed in the field nurseries under exposure to the inoculum of the Indian virulent races of leaf rust. The seedling data demonstrated that both the substitution lines and the backcross derivatives for each gene carry identical pattern of infection for resistance. The high level of adult plant resistance in the substitution lines, in contrast to the backcross derivatives in Thatcher, has been postulated to be due to the combination of resistance contributed by Lr10 and adult plant Chinese Spring resistance or to Lr23 and Chinese Spring adult plant resistance. It has been suggested that genes Lr10 and Lr23 added to the Chinese Spring background provide sources for durable resistance, since Chinese Spring has continued to provide a moderate level of adult plant resistance to leaf rust for a very long time.
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    Standort Signatur Erwartet Verfügbarkeit
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  • 24
    Digitale Medien
    Digitale Medien
    Springer
    Theoretical and applied genetics 78 (1989), S. 521-524 
    ISSN: 1432-2242
    Schlagwort(e): Triticum aestivum ; SCEs ; Auxin ; Cytokinin ; Tissue culture
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary In order to know the mutagenic effects of synthetic auxins (NAA, 2,4-D, and 2,4,5-T) and a cytokinin (kinetin) in vitro, sister chromatid exchanges (SCEs) were analyzed in cultured cells of a hexaploid wheat (Triticum aestivum L.). In the MS medium supplemented with 2.0 mg/l 2,4-D, the mean number of SCEs per cell was 15.2, and per pg of DNA, 0.42. No significant effect was found in the treatments of NAA or 2,4-D at concentrations of 0.5–10.0 mg/l, whereas more than 2.0 mg/l of 2,4,5-T induced dramatic increases of SCEs. Kinetin itself had no significant effect on SCE induction, but there was a tendency that SCEs induced by 2,4,5-T were suppressed by kinetin.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 25
    Digitale Medien
    Digitale Medien
    Springer
    Theoretical and applied genetics 78 (1989), S. 625-632 
    ISSN: 1432-2242
    Schlagwort(e): Triticum aestivum ; Tissue culture ; Callus ; Monosomic analysis ; Regeneration
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The ability of immature embryos of wheat (Triticum aestivum L.) to respond in cell culture was examined in crosses between the ‘Wichita’ monosomic series and a highly regenerable line, ‘ND7532’. Segregation in disomic controls and 13 monosomic families showed a good fit to a monogenic ratio indicating a qualitative mode of inheritance. Segregation in the cross involving monosomic 2D showed a high frequency of regeneration (93.6%) and high callus growth rate (1.87 g/90 days) indicating that 2D is a critical chromosome. Modifying genes may be located on other chromosomes. Substitution of chromosomes from a low regenerable cultivar ‘Vona’ further indicated that the group 2 chromosomes, in particular chromosome 2D, possess genetic factors promoting callus growth and regeneration.
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    Standort Signatur Erwartet Verfügbarkeit
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  • 26
    ISSN: 1617-4623
    Schlagwort(e): Drosophila ; Vestigial ; Dihydrofolate reductase ; Aminopterin
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Vestigial (vg) mutants of Drosophila melanogaster are characterized by atrophied wings. In this paper we show that: (1) aminopterin an inhibitor of dihydrofolate reductase (DHFR) and fluorodeoxyuridine (FUdR), an inhibitor of thymidylate synthetase induce nicks in the wings of wild-type flies and phenocopies of the vg mutant phenotype when vg/+ and vg B/+ flies are reared on these substances (vgB is a deficiency of the vg locus). Only thymidine and thymidylate can rescue the flies from the effect of aminopterin. We propose that the vg phenotype is due to a decrease in the dTMP pool in the wings. (2) Mutant vg strains yield more offspring on medium containing aminopterin than on normal medium. The resistance of vg larvae to the inhibitor seems specific to the gene. This is the first case of aminopterin resistance in living eucaryotes. In contrast sensitivity of the vg larvae to FUdR is observed. (3) An increase in the activity and amount of DHFR is observed in mutant strains as compared with the wild-type flies. Our data suggest that the vg + gene is a regulatory gene acting on the DHFR gene or a structural gene involved in the same metabolic pathway.
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    Standort Signatur Erwartet Verfügbarkeit
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  • 27
    Digitale Medien
    Digitale Medien
    Springer
    Molecular genetics and genomics 218 (1989), S. 118-126 
    ISSN: 1617-4623
    Schlagwort(e): Drosophila ; Regulation ; yellow gene ; Germline transformation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary We have assessed the DNA sequence requirements for the correct spatial pattern and phenotypic expression of y in the late embryo/larvae. The wild-type larval phenotype requires both the regions between-294 bp and-92 bp and a portion of the intron; the sequence element(s) located within the intron can act in a position independent manner to effect the wild-type larval phenotype. The larval expression pattern was examined by tissue experiments in situ and by staining germline transformants derived from various y/lacZ fusion constructs. The larval expression of y is restricted to the mouthparts, microsetae and anal plates. While the-495 bp to+194 bp region alone cannot effect a wild-type larval expression pattern, this region in conjunction with the intron appears to be sufficient to drive β-gal expression in an essentially wild-type pattern. Our data further suggest that the-294 bp to-92 bp region contains elements which specify the larval pattern and that the element(s) in the intron normally act to enhance the level of expression necessary for the wild-type larval phenotype. We also present a phenotypic analysis of the adult cuticle structures of germline transformants derived from a variety of deletion and rearrangement constructs of the y gene. This analysis has revealed several new features associated with the regulation of y expression.
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    Standort Signatur Erwartet Verfügbarkeit
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  • 28
    Digitale Medien
    Digitale Medien
    Springer
    Molecular genetics and genomics 215 (1989), S. 281-285 
    ISSN: 1617-4623
    Schlagwort(e): Drosophila ; achaete-scute complex ; myc ; Protein domains ; Genomic search
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Several genes of the achaete-scute complex (ASC) of Drosophila melanogaster encode a 60 amino acids long conserved domain which shares a significant homology with a region of the vertebrate myc proteins. Based on these results, the existence of a family of Drosophila genes that would share both this conserved domain and the neurogenic function of the AS-C has been postulated. To test this proposal, we have searched a D. melanogaster genomic library with a probe that encodes the conserved domain. Only under very low stringency hybridization conditions, clones not belonging to the AS-C cross-hybridized with the probe. Those that gave the strongest signals were characterized. Sequencing of the cross-hybridizing regions showed that they had no significant homology with the conserved domain, the sequence similarity extending at the most for 37 nucleotides. Although our results do not conclusively disprove the existence of a family of AS-C-like genes, they indicate that the conservation of the domain would be lower than that found for shared motifs in other families of Drosophila developmental genes.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 29
    Digitale Medien
    Digitale Medien
    Springer
    Protoplasma 152 (1989), S. 14-21 
    ISSN: 1615-6102
    Schlagwort(e): Differentiation ; Heterochronic lysis ; Polarity ; Root protophloem sieve elements ; Triticum aestivum ; Ultrastructure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Developing protophloem sieve elements in roots of wheat are arranged in single vertical files. In the last immature differentiating sieve element bearing ribosomes the proximal end of the cytoplasm displays a diluted appearance in contrast to the distal end where the cytoplasm exhibits a considerably increased electron density. Differences can also be observed in ribosome quantity, organelle ultrastructure and the time of initiation of cell component degradation, those at the proximal end disorganizing first, suggesting a nonsimultaneous disorganization of the cell components in the two areas. This phenomenon, termedheterochronic lysis, is presumably an expression of an existing polarity not detectable in younger stages, but it might also be the result of an asynchronous enzymatic activity.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 30
    Digitale Medien
    Digitale Medien
    Springer
    Evolutionary ecology 3 (1989), S. 31-39 
    ISSN: 1573-8477
    Schlagwort(e): Genetic elements ; isozymes ; life span ; Drosophila
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Analysis of electrophoretic loci shows that at least four differences exist in isozymes of long- and short-lived populations ofD. melanogaster, descended by selection from a common ancestral stock. Adults of longlived populations differ in gene dosage of phosphoglucomutase (PGM), NAD malate dehydrogenase (MHD), NADP malic enzyme (ME) and by additional mobility variants of glucose-6-phosphate dehydrogenase (G6PD). Larvae, however, differ only by variants of G6PD. The differences in these enzymes, considered together with the greater flight endurance that long-lived populations have shown elsewhere, suggest that increased glycogen synthesis plays a significant role in the improved life span of selected populations. Adaptation to selection for increased life span may, therefore, derive from an improved ability to use dietary sucrose in the media provided. The distribution of electrophoretic loci agrees with the results of a study indicating the position of genetic elements contributing to life span.
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    Standort Signatur Erwartet Verfügbarkeit
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  • 31
    Digitale Medien
    Digitale Medien
    Springer
    Evolutionary ecology 3 (1989), S. 189-201 
    ISSN: 1573-8477
    Schlagwort(e): Developmental homeostasis ; life history traits ; Drosophila ; breeding site variation ; cactus ; Sonoran desert
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Variation in life histories among populations of cactophilicDrosophila mojavensis has been hypothesized to be a by-product of a shift to one of two alternate host plants. When cultured on the ancestral and a secondary host cactus, a Baja population expressed shorter development times and smaller thorax sizes than a mainland population, but viability did not differ. Comparisons with all reciprocal F1 and F2 crosses between populations revealed that genetic differences in development time and thorax size were largely additive. Homeostasis in these life history traits was population specific, except for viability. Homeostasis in development time was greater in the Baja population than in the other crosses, suggesting dominance for decreased homeostasis in the mainland population. Underdominance in viability homeostasis of the F1 hybrids suggested some incompatibility between populations. Homeostasis in thorax size was greater in females than in males and differed among parental populations. Maintenance of heritable differences and genetic variation for homeostasis in these traits suggested a role for cactus-specific differences in environmental uncertainty caused by variation in breeding site duration and abundance in nature.
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    Standort Signatur Erwartet Verfügbarkeit
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  • 32
    Digitale Medien
    Digitale Medien
    Springer
    Plant and soil 119 (1989), S. 199-204 
    ISSN: 1573-5036
    Schlagwort(e): copper sources ; grain yield ; methods of application ; park wheat ; stem melanosis ; Triticum aestivum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Park wheat (Triticum aestivum cv. Park) is susceptible to stem melanosis when grown on Cu-deficient soils. Three field experiments were conducted to evaluate the effectiveness of various Cu sources, using different methods and rates of application for reducing the incidence of stem melanosis and increasing the grain yield of Park wheat on a Cu-deficient Black Chernozemic soil in central Alberta. A fourth experiment determined the residual effect of Cu over 4 years. In the first experiment, Park wheat had less disease and higher grain yield when Cu-chelate solution at 2 and 4 kg Cu ha−1 or Cu-sulfate solution at 10 and 20 kg Cu ha−1 was applied to the soil surface and incorporated to a depth of 8 cm or was applied at these rates as a foliar spray. The foliar application, however, was phytotoxic and delayed maturity by approximately two weeks. Sidebanding Cu (4 cm to the side and 4 cm below the seed row) was least effective. In the second experiment, Cu-sulfate solution incorporated into the soil was more effective than the soil incorporated granular Cu-sulfate in reducing disease incidence and increasing grain yield in the year of application. In the following year the granular Cu was as effective as solution Cu. The third experiment showed that Cu seed dressings did not have any effect on disease incidence or grain yield of Park wheat. In the fourth experiment, the residual effect of Cu-chelate was evident four years after application. The grain yield in the fourth year was about four times that of the control.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 33
    ISSN: 1617-4623
    Schlagwort(e): Chromosomal walking ; Cut locus ; Drosophila ; Unstable mutations
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract We have cloned from the Oregon R strain of Drosophila melanogaster a 240 kb segment of DNA that contains the cut (ct) locus, and characterized the region for the presence of repetitive elements. Within this region at least five copies of the suffix element were detected, as well as several putatively novel mobile elements. A number of mutations obtained from the unstable ct MR2 strain and its derivatives were mapped within the cut locus. Comparison between parental and daughter strains indicates that frequently two or more independent transposition events involving the cut locus occur simultaneously within a single germ cell, thus providing a molecular basis for the transposition explosion phenomenon.
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    Standort Signatur Erwartet Verfügbarkeit
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  • 34
    Digitale Medien
    Digitale Medien
    Springer
    Molecular genetics and genomics 219 (1989), S. 397-403 
    ISSN: 1617-4623
    Schlagwort(e): Drosophila ; Pyrimidine biosynthesis ; Dihydroorotate dehydrogenase ; Molecular mapping
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The dhod locus encodes dihydroorotate dehydrogenase, the fourth enzymatic step of de novo pyrimidine biosynthesis. This locus was cloned previously by a chromosome walk in cytogenetic region 85A. The location of dhod within 85A DNA has been determined by mapping two rearrangement mutations to a small DNA region. A nearly full-length cDNA clone of the dhod transcript was isolated and partially sequenced, to confirm its identity. The cDNA clone was also used to map the transcribed DNA. A 1.5 kb dhod RNA is described which is most abundant in embryos and displays minor length heterogeneity in pupae and adults. The developmental expression of this transcript is discussed relative to the expression of dihydroorotate dehydrogenase activity and other genes of the pyrimidine biosynthetic pathway.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 35
    Digitale Medien
    Digitale Medien
    Springer
    Molecular genetics and genomics 215 (1989), S. 190-199 
    ISSN: 1617-4623
    Schlagwort(e): Mutagen sensitivity ; Recombination ; Gene structure ; Drosophila ; mei-41
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The mutagen-sensitive mutant mus(1)104 D1 of Drosophila melanogaster maps to a position on the X chromosome very close to the meiotic mutant mei-41 D5 . Both mutants have been characterized as mutagen-sensitive and defective in post-replication repair. In the present report we show by complementation studies that mus(1)104 and mus(1)103 are allelic with mei-41. In addition, two reported alleles of mus(1)104 lie between the mei-41 alleles A10 and D5. The size of the mei-41 locus is estimated to be about 0.1 centimorgans (cM). Because several alleles of mei-41 have been shown to reduce recombination and increase meiotic chromosome loss and nondisjunction, mus(1)104 D1 females were examined for defects in meiosis. Although there was no evidence for reduced recombination on the second chromosome in homozygous mus(1)104 D1 females, heterozygous mus(1)104 D1 /mei-41 〉D5 and mus(1)104 D1 /deficiency females showed reduced levels of recombination. However, there was no evidence of an increase in nondijunction in these females.
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    Standort Signatur Erwartet Verfügbarkeit
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  • 36
    Digitale Medien
    Digitale Medien
    Springer
    Molecular genetics and genomics 215 (1989), S. 257-265 
    ISSN: 1617-4623
    Schlagwort(e): Drosophila ; Maternal effect ; Syngamy ; Embryonic development ; Molecular cloning
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The maternal effect locus fs(1) Ya is required for the fusion of the apposed sperm and egg pronuclei (syngamy) following fertilization in Drosophila. It is tightly linked to another complementation group, fs(1) Yb, needed for both oogenesis and embryogenesis. We have isolated a set of overlapping cloned sequences in the 3B4-6 region of the X chromosome encompassing the fs(1) Ya-fs(1) Yb region. A single 2.4 kb maternal transcript is encoded with-in this region, and an 8.5 kb DNA fragment that contains this transcript complements both fs(1) Ya and fs(1) Yb mutations. Northern and in situ hybridization analyses show that the maternal transcript is only present in nurse cells and oocytes beginning in previtellogenic stages, and is evenly distributed in the cytoplasm of 0–2 h syncytial embryos. The transcript is not detected in later stages of embryonic development. This expression pattern correlates closely with the genetic and developmental characteristics expected of the fs(1) Ya gene product.
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    Standort Signatur Erwartet Verfügbarkeit
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  • 37
    Digitale Medien
    Digitale Medien
    Springer
    Molecular genetics and genomics 215 (1989), S. 469-477 
    ISSN: 1617-4623
    Schlagwort(e): Drosophila ; Y chromosome ; Repetitive DNA ; Testis RNA ; Transposable elements
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary A cDNA clone bank was constructed from testis poly(A)+ RNA of Drosophila hydei and screened for clones which hybridize to Y chromosomal DNA sequences. The insert of clone cDhT14 hybridizes to a family of repeated DNA sequences with members distributed within the Y chromosome and elsewhere in the genome. This type of sequence has earlier been described as the Y-associated class of DNA. Southern blot analysis of DNA from different wild-type strains of D. hydei suggests that members of the T14 family of repeated DNA sequences are parts of a family of transposable elements. The genomic localization of the T14 family of repeated DNA sequences was revealed by in situ hybridization to metaphase and polytene chromosomes, and to transcripts of Y chromosomal lampbrush loops. Approximately 10–15 members (20%–30%) of the T14 sequence family reside in 8.3 kb PstI restriction fragments. A genomic clone of one of these DNA fragments, DhT14-8.3, hybridizes to transcripts on the Y chromosomal lampbrush loop “cones”, and in conventional in situ hybridization experments to region 12D/13A of the X chromosome and to region 112 of chromosome 5. The cDNA clone cDhT14 represents a part of an abundant testis RNA species of 5.0 kb. This RNA is also present in ovaries and in 0–3 h, 3–6 h and 6–12 h embryos, but less abundantly than in testes. Both the Y chromosomal site of the 8.3 kb PstI fragments and sites elsewhere in the genome are actively transcribed. At least one of the latter genomic sites is transcribed into the 5.0 kb RNA species. This poly(A)+ RNA is present in the cytoplasm of primary spermatocytes as shown by transcript in situ hybridization. The potential function of transcripts from Y chromosomal lampbrush loops is discussed.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 38
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 40 (1989), S. 161-167 
    ISSN: 1573-5060
    Schlagwort(e): Triticum aestivum ; bread wheat ; near-isogenic lines ; phenotypic similarity
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary The degree of phenotypic similarity of Centana and its near-isogenic lines (NILs) has been investigated. Some NILs have a disease reaction, a gliadin pattern and a yield level which differs from Centana. This means that they still possess many donor genes. One of the NILs, Shortana, must be a derivative of a backcross line crossed with an unknown genotype resulting in a good yielding variety.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 39
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 40 (1989), S. 259-264 
    ISSN: 1573-5060
    Schlagwort(e): Triticum aestivum ; bread wheat ; Triticum turgidum durum ; durum wheat ; pentaploid hybrids ; homoeologous pairing
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary The meiotic behavior of 22Triticum turgidum durum × Triticum aestivum L. cv. Chinese Spring (2n=35 chromosomes, AABBD genomes) F1 hybrids has been analyzed and compared with that of the same cultivars hybridized withT. turgidum durum cv. Senatore Capelli. The multivalent associations observed in the hybrids may be attributed to a large extent to homoeologous pairing. Our study shows that not all the chromosomes of the D genome have the same affinity with their homoeologues of the A or B genomes. We have also concluded that there is no correlation between the percentage of the cells with multivalent associations and the mean of chiasmata per cell (attached arm).
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 40
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 41 (1989), S. 235-246 
    ISSN: 1573-5060
    Schlagwort(e): Triticum aestivum ; bread wheat ; landrace groups ; classification ; primary landrace ; secondary landrace ; Austria
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Using 49 morphological characteristics sixty-six accessions originating from 15 populations of bread wheat, together with 8 selections from landraces and 5 improved varieties were classified in five groups: group 1: Alpiner Bartwizen (including Hausbergweizen), Attergauer Bartweizen, which are marked by awned white ears; group 2: Awned Sipbachzeller (and its selection Otterbacher Bartweizen) and marked by awned red ears. This group also includes some awned derivatives of hybrids between group 1 and group 3; group 3: Sipbachzeller wheat, including Innviertler wheat and the selections Ritzlhofer Alt, Achleitner and Wieselburger roter Kolben; group 4: Tassilo and Tassilo-variants, and group 5: Dickkopf (Squarehead) types with lax ear, probably being derivatives of hybrids between group 3 and Dickkopf types. Most populations were contaminated with foreign material. The contamination may have been caused by the farmer who actually mixed varieties, or may have originated on the farmer's field.
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    Standort Signatur Erwartet Verfügbarkeit
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  • 41
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 42 (1989), S. 41-44 
    ISSN: 1573-5060
    Schlagwort(e): Triticum aestivum ; wheat ; frost resistance ; locus Fr1 ; recombinant lines ; chromosome 5A
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary A gene for frost resistance on chromosome 5A of wheat was located using single chromosome recombinant lines from the cross between the substitution line Hobbit (Triticum spelta 5A) and Hobbit. In this sample of recombinant lines the locus for frost resistance, designated Fr1, is completely linked to the locus Vrn1 controlling vernalisation requirement. The results can be explained by a pleiotropic action of the Vrn1 locus or close genetic linkage between Vrn1 and Fr1. Further detailed study is necessary to resolve these alternative hypotheses.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 42
    ISSN: 1573-5060
    Schlagwort(e): Triticum aestivum ; wheat ; dwarfing genes ; drought stress ; yield ; yield components
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Near-isogenic tall (no dwarfing gene), semidwarf (Rht1 or Rht2) and dwarf (Rht1 + Rht2 or Rht3) spring wheat lines were evaluated for yield and yield components under irrigated and rainfed conditions. Under irrigated conditions, the dwarf and the semidwarf lines exhibited a significant yield advantage over the tall lines. Under rainfed conditions, the semidwarf lines outyielded the tall as well as the dwarf lines. Percent yield reduction in response to drought stress was highest with the dwarfs and lowest with the tall lines. Dry matter production of the tall lines and that of the semidwarf lines did not differ significantly and both produced significantly more dry matter than the dwarf lines under irrigated as well as rainfed conditions. Plant height and kernel weight decreased with increasing degree of dwarfness while number of kernels per spikelet, harvest index and days to heading increased under both moisture regimes. The dwarfing genes did not have any significant influence on number of tillers/m2 and spikelets per spike in either moisture regime.
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    Standort Signatur Erwartet Verfügbarkeit
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  • 43
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 43 (1989), S. 41-45 
    ISSN: 1573-5060
    Schlagwort(e): Triticum aestivum ; wheat ; chromosome substitution ; frost resistance ; hardening
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary The highly frost resistant wheat variety Cheyenne (donor) and the poorly frost resistant variety Chinese Spring (recipient) were frozen at −9° C and −11° C at various stages of hardening, as were a number of substitution lines of these two varieties (CS/Ch 3A, CS/Ch 5A, CS/Ch 7A, CS/Ch 2B, CS/Ch 4B, CS/Ch 5B, CS/Ch 4D, CS/Ch 5D). Chromosomes 5A, 5B, 5D, 4B and 7A of Cheyenne increased the frost resistance of the recipient variety to varying extents. However, the frost resistance changed not only as a function of the different chromosomes, but also as a function of the duration of hardening, indicating that genes responsible for frost resistance are expressed differently during different phases of the hardening process.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 44
    ISSN: 1573-5060
    Schlagwort(e): Triticum durum ; durum wheat ; Triticum aestivum ; wheat germplasm ; collection ; yield components ; drought response ; canopy temperature ; osmotic adjustment ; phenology ; Israel
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Diverse landraces of wheat, collected from the semi-arid (150 to 250 mm of total annual rainfall) Northern Negev desert in Israel were considered as a potential genetic resource of drought resistance for wheat breeding. These materials were therefore evaluated for their reponses to drought stress in agronomical and physiological terms. Up to 68 landraces, comprising of Triticum durum, T. aestivum, and T. compactum were tested in two field drought environments, in one favourable field environment, under post-anthesis chemical plant desiccation which revealed the capacity for grain filling from mobilized stem reserves, under a controlled drought stress in a rainout shelter and in the growth chamber under polyethylene glycol (PEG)-induced water stress. Biomass, grain yield and its components, harvest index, plant phenology, canopy temperatures, kernel weight loss by chemical plant desiccation, growth reduction by PEG-induced drought stress and osmotic adjustment were evaluated in the various experiments. Landraces varied significantly for all parameters of drought response as measured in the different experiments, which was in accordance to their documented large morphological diversity. Variation in grain yield among landraces under an increasing drought stress after tillering was largely affected by spike number per unit area. Kernel weight contributed very little to yield variation among landraces under stress, probably because these tall (average of 131 cm) landraces generally excelled in their capacity to support kernel growth by stem reserve mobilization under stress. Yield under stress was reduced with a longer growth duration of landraces only under early planting but not under late planting. Landraces were generally late flowering but they were still considered well adapted phenologically to their native region where they were always planted late. Landraces differed significantly in canopy temperature under drought stress. Canopy temperature under stress in the rainout shelter was negatively correlated across landraces with grain yield (r=0.67**) and biomass (r=0.64**) under stress. Canopy temperature under stress in the rainout shelter was also positively correlated across landraces (r=0.50**) with canopy temperature in one stress field environment. Osmotic adjustment in PEG-stressed plants was negatively correlated (r=−0.60**) with percent growth reduction by PEG-induced water stress. It was not correlated with yield under stress in any of the experiments. In terms of yield under stress, canopy temperatures and stem reserve utilization for grain filling, the most drought resistant landrace was the ‘Juljuli’ population of T.durum.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 45
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 44 (1989), S. 65-72 
    ISSN: 1573-5060
    Schlagwort(e): Triticum aestivum ; wheat ; Puccinia recondita ; leaf rust resistance ; alien gene transfers
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Nine transfers of leaf rust (Puccinia recondita Rob. ex Desm.) resistance to wheat (Triticum aestivum L.) from Agropyron elongatum Host. Beauv., Triticum speltoides Tausch and rye (Secale cereale L.) were backcrossed up to 10 times to commercial wheat cultivars. The objective was to study the effect of the transfers on agronomic and quality characters and to make them available in desirable genetic backgrounds. The results varied greatly for different transfers. In four cases no promising material was obtained even after nine backcrosses. However, for the remaining five transfers material with potential as a new cultivar was obtained.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 46
    ISSN: 1573-5060
    Schlagwort(e): Triticum aestivum ; wheat ; Puccinia recondita f. sp. tritici ; wheat leaf rust ; partial resistance ; latency period ; gene action ; oligogenic inheritance ; transgressive segregation ; number of genes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Crosses were made between the highly susceptible Little Club and the partially resistant cultivars Westphal 12A, Akabozu and BH 1146 to obtain F1, F2 and backcross generations. Latency period (LP) was determined in plants inoculated at the young flag leaf stage with a monospore culture of race ‘Flamingo’ of wheat leaf rust. Broad sense heritability of LP in the F2 averaged 0.8. The genes showed partial to almost complete recessive inheritance. Scaling tests indicated that additive gene action was the most important factor in the inheritance of partial resistance. The tests showed that there were no indications for additive x additive, additive x dominance or dominance x dominance interactions. The number of effective factors was estimated as one or two for Akabozu, three or more for Westphal 12A, and two or three for BH 1146. BH 1146 also possessed a (semi-)dominant gene for a lower infection type which was temperature sensitive in its expression. The genes of the various parents had unequal effect on LP.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 47
    ISSN: 1573-5060
    Schlagwort(e): Triticum aestivum ; wheat ; Puccinia recondita f. sp. tritici ; wheat leaf rust ; partial resistance ; latency period ; oligogenic inheritance ; transgressive segregation ; number of genes ; heritability
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Three partially resistant spring wheat cultivars, with a long latency period were crossed among each other and with the highly susceptible Little Club, with a very short latency period. Parents, F3 and F5 plants have been inoculated with the leaf rust race Flamingo in the young flag leaf stage to determine the latency period. From the crosses with Little Club, it was concluded that Westphal 12A carries three, Akabozu two and BH 1146 two or three genes for a longer latency period. BH 1146 appears to carry also one hypersensitive resistance gene. Transgressive segregation occurred in crosses between partially resistant cultivars. From crosses between the partially resistant cultivars, it was concluded that the genes in Akabozu and Westphal 12A are different, while those in Akabozu and BH 1146 are at least partly different. The possibilities of accumulation of LP-prolonging genes are discussed.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 48
    ISSN: 1573-5060
    Schlagwort(e): Triticum aestivum ; wheat ; anther culture ; callus initiation ; cytoplasm ; cytoplasmic male sterility
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Nuclear and cytoplasmic factors affect tissue culture response in wheat (Triticum aestivum), and cytoplasmic male sterility may enhance callus initiation in anther culture. Three wheat nuclear genotypes, each in normal and two alien cytoplasms conferring cytoplasmic male sterility, were evaluated for callus initiation frequency in anther culture. Nuclear genotype had the greatest effect on callus initiation, but cytoplasm and nucleus X cytoplasm interaction also produced significant effects. The nuclear genotype of ‘Chris’ outperformed ‘Butte’ and ‘Coteau’ in all cytoplasms. Ordinary wheat and Triticum timopheevi cytoplasms outperformed Aegilops speltoides cytoplasm. Cytoplasmic male sterility did not increase callus initiation. This suggests manipulating the nuclear genotype is the best strategy for improving the capacity of wheat to initiate callus in anther culture.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 49
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 40 (1989), S. 103-109 
    ISSN: 1573-5060
    Schlagwort(e): Triticum aestivum ; wheat ; triticale ; wheat-triticale hybrids ; Secale cereale ; rye ; Gaeumannomyces graminis ; take-all ; resistance
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Fifteen triticale and wheat-triticale hybrid lines were evaluated for resistance to the take-all fungus Gaeumannomyces graminis var. tritici and compared with five wheat and two rye lines in inoculated field and pot trials. The triticale and wheat-triticale hybrid lines varied in rye chromosome number and degree of resistance expressed. One line, Venus with seven pairs of rye chromosomes consistently showed levels of resistance intermediate between wheat and rye. A trend was observed where increasing rye chromosome content led to greater resistance but exceptions showed that variation within triticales could not be ascribed to rye chromosome content alone.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 50
    ISSN: 1573-5060
    Schlagwort(e): Triticum aestivum ; wheat ; gliadins ; electrophoresis ; cultivar identification ; breeding
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary The process of the separation of gliadin proteins of wheat, using polyacrylamide gel electrophoresis, was studied in detail. It was shown that electrophoresis is a dynamic process during which the pH of the gel changes together with the potassium ion concentration. The most positive effect on the separation of the gliadins was generated by a moving front, which is a boundary between regions with a low concentration of K+ ions and a low pH, and region with a high concentration of K+ ions and a high pH, after optimization of the concentrations of the cations and anions in the electrode solutions. The finding was exploited for the development of an extremely simple electrophoresis system, in which buffers were not needed for obtaining a high resolution. The system was further improved by applying a stacking gel. The advantages of this system are discussed. This new approach may be useful for improving electrophoresis systems for other applications.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 51
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 43 (1989), S. 53-57 
    ISSN: 1573-5060
    Schlagwort(e): Triticum aestivum ; bread wheat ; gibberellic acid insensitivity ; identification of dwarfing genes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary GA-insensitive Rht genes of 18 Japanese modern varieties and landraces were identified. Out of 12 modern varieties tested 6 carried only Rht1, and the other 6 carried only Rht2. No varieties carried both Rht1 and Rht2 or Rht3. The geographical distribution of the Rht genotypes in the Japanese modern varieties was clearly localized. All 6 landraces tested carried only Rht2.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 52
    ISSN: 1573-5060
    Schlagwort(e): Triticum aestivum ; Scalavatis bread wheat ; durum-like hexaploid wheat ; contamination ; variation ; groups ; cluster analysis ; relationship ; Cyprus
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary The mutual resemblance of 81 hexaploid wheat plants contaminating durum fields on Cyprus was investigated by determining their phenotypes of gliadins, glutenins and peroxidase. Three groups were generated by clustering programs. Group 1 consists of plants with ears resembling those of durum plants, plants of groups 2 and 3 have a bread wheat appearance. Suggestions on the origin of these three groups are made. Group 1 may derive from (back)crosses between hexaploid and durum plants since time immemorial. If so, the hexaploid plants would have served as ‘donor’ of the D genomes, and durum plants as the ‘recurrent parent’. Groups 2 and 3 may derive from two introductions, either from two countries, or from the same country, but then in different periods.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 53
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 44 (1989), S. 143-150 
    ISSN: 1573-5060
    Schlagwort(e): Triticum aestivum ; wheat ; Triticum durum ; durum ; environmental correlations ; grain yield ; plant height ; tiller number ; grain size
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Environmental correlation coefficients were computed among all pairs of five traits, namely grain yield, heading date, number of tillers per m2, plant height and 1000-grain weight (grain size) using 30 Triticum durum and 30 Triticum aestivum varieties grown in 18 environments. Grain yield was significantly correlated with the other four traits in almost all of the varieties. The mean correlation coefficient over all varieties ranged from 0.58 to −0.83 for durum wheat and 0.66 to 0.88 for aestivum wheat. The correlation coefficients between heading date and the other traits were also significant, ranging from −0.45 to −0.79 in durum wheat and −0.61 to −0.85 in aestivum wheat. The correlation coefficient between number of tillers with plant height and 1000-grain weight were the smallest, 0.19−0.32 in durum wheat and 0.39−0.60 in aestivum wheat. It was concluded that agronomic practices favouring early and good stand establishment in the dry regions will favour the yield components and important adaptive traits, which contribute towards larger yields. Significant differences were found among genotypes in the environmental correlation coefficients and the associated changes in one trait as a result of changes in other traits.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 54
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 44 (1989), S. 187-195 
    ISSN: 1573-5060
    Schlagwort(e): Triticum aestivum ; wheat ; Puccinia recondita f. sp. tritici ; wheat leaf rust ; partial resistance ; latency period ; infection frequency ; urediosorus size ; growth stage ; component analysis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Latency period (LP), infection frequency (IF) and urediosorus size (US) of leaf rust were determined on primary leaves and young flag leaves of 18 spring wheat cultivars. A large growth stage effect and a large cultivar effect on all three components were observed. Partial resistance as measured by the three components was generally better expressed in the adult plant stage than in the seedling stage. Associated variation of the components was observed: long LP, low IF and small US tended to go together. The association was not complete, cultivars with clear deviations of this association for one of the components were found suggesting the existence of at least partly different genetic factors controlling the respective components. LP measured on flag leaves gave the most reliable results and, therefore, could best be used as a selection criterion in breeding programs for partial resistance.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 55
    ISSN: 1573-5060
    Schlagwort(e): Triticum aestivum ; wheat ; Puccinia recondita f.sp. tritici ; leaf rust ; temperature sensitivity ; latency period ; infection frequency ; urediosorus size ; growth stage
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Three components of partial resistance (PR) were studied at three post-infection temperatures using seven spring wheat genotypes differing in level of PR and two different wheat leaf rust races. The components were latency period (LP), infection frequency (IF) and urediosorus size (US). The expression of LP was more sensitive to temperature than the expression of the other two components. LP-prolonging genes were better expressed at low temperatures than at high temperatures and cultivar differences tended to increase with decreasing temperature in both seedling and adult plant stages. The reaction of IF to temperature differed from that of LP and US, probably because IF is regulated by another mechanism than LP and US. It is recommended to perform PR-screening tests at low rather than at high temperatures. If temperatures are maintained at about 8–13°C (night-day), seedlings can be used to screen for PR instead of the more expensive adult plant tests. The effectiveness of PR in seedling stage at low temperatures suggests that the seedling stage may have epidemiological significance as the low temperatures (8–13°C) are relevant for seedlings in the field.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 56
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 44 (1989), S. 225-234 
    ISSN: 1573-5060
    Schlagwort(e): Triticum aestivum ; winter wheat ; breeding ; phenology ; vernalization ; Vrn genes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary An international survey of wheat breeders and scientists was conducted to determine whether there was a common understanding of the term ‘winter wheat’. Response to photoperiod was mentioned by only seven of the 30 survey respondents. It was consequently suggested that it be excluded from any definition. Response to vernalization was mentioned by 28 of the 30 respondents. However, there was considerable variation in the method of quantifying this response and the degree of response necessary for wheat to be called ‘winter’. It was concluded that the only objective and internationally consistent definition of ‘winter wheat’ was a ‘genetic’ definition based on the complement of Vrn genes a wheat possesses. Varieties not possessing any of the dominant Vrn alleles appear to take a distinctively longer and more variable time to head when grown under non-vernalizing, long day conditions. These are ‘genetically winter’ wheats.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 57
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 44 (1989), S. 273-282 
    ISSN: 1573-5060
    Schlagwort(e): Triticum aestivum ; wheat ; race-specificity ; Puccinia recondita f.sp. tritici ; wheat leaf rust ; partial resistance ; hypersensitive resistance ; latency period ; disease severity
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Partial resistance (PR) in wheat to wheat leaf rust (Puccinia recondita f.sp. tritici) is characterized by a slow epidemic build-up despite a susceptible infection type. Two greenhouse tests and two field tests, in which 11 spring wheat cultivars were exposed to five wheat leaf rust races, revealed some indication for race-specificity of PR. In the greenhouse, the expression of PR was highly dependent on the environment. Significant cultivar-race interactions in the first experiment were lost in the second experiment probably due to cultivar-environment and cultivar-race-environment interactions. In the polycyclic field tests several factors played a role in explaining the inconsistency of the cultivar-race interactions, such as differences in initial inoculum, genotypic differences in earliness, interplot interference or environmental conditions. One cultivar-race combination showed a significant but small interaction towards susceptibility in both field experiments. The interaction was probably too small to detect in the monocyclic greenhouse tests. The results do not conflict with the idea that a gene-for-gene relationship could exist between PR-genes in the host and genes in the pathogen. Some problems with regard to the selection of PR in wheat to wheat leaf rust are discussed.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 58
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 44 (1989), S. 241-245 
    ISSN: 1573-5060
    Schlagwort(e): Triticum aestivum ; wheat ; Puccinia recondita f.sp. tritici ; leaf rust ; partial resistance ; environmental stability
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Five spring wheat cultivars differing in partial resistance (PR) to wheat leaf rust were tested at Wageningen (the Netherlands) on a sandy and a clay site, El Batan (CIMMYT, Mexico) and Ponta Grossa (Brazil) over two years. The cultivars were Skalavatis 56, Little Club (both very susceptible), Westphal 12A, Akabozu and BH 1146 (all three with high levels of PR). The results showed that PR was expressed at all four locations in both years. The level of expression was influenced by the environment but the cultivar ranking was hardly affected. Selection for PR in the field can therefore be carried out over a wide range of environments.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 59
    Digitale Medien
    Digitale Medien
    Springer
    Euphytica 44 (1989), S. 247-258 
    ISSN: 1573-5060
    Schlagwort(e): Triticum aestivum ; wheat ; Puccinia recondita f.sp. tritici ; leaf rust ; epidemiological parameters ; microfield ; race nursery ; partial resistance ; components of resistance
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Eighteen spring wheat cultivars were tested in microfields and race nurseries for their partial resistance PR to wheat leaf rust under low and high disease pressure respectively. Large differences existed between the 18 cultivars, Skalavatis 56 being the most susceptible and Ponta Grossa 1 being the most resistant cultivar. Of the three epidemic parameters, disease severity (DS) at the time that the susceptible check was severely diseased and area under the transformed disease severity curve (AUTC) and the logistic growth rate (r), AUTC and DS were highly correlated. Both seemed to be reliable estimators of PR but DS should be preferred for economical reasons. The logistic growth rate seemed to be unsuitable as an estimator of partial resistance. High and low disease pressure gave similar cultivar ranking. PR can be screened and selected equally well in race nurseries with low space, low time and low cost input as in microfields with high space, time and cost input. Cultivar differences in development rate had a large impact on the cultivar differences for amount of disease and can therefore greatly bias the estimation of cultivar resistance. The resistance of early cultivars tended to be underestimated whereas the resistance of late cultivars tended to be overestimated. The effect of differences in developmental rate was most pronounced in the flag leaf. It is advisable to avoid the assessment of disease levels on the flag leaf only and to incorporate in the tests several susceptible and resistant checks that cover the range of development rates in the material to be selected, because otherwise selection for resistance will tend to select also for lateness. Regression of the epidemiological parameters on three components of partial resistance revealed that latency period (LP) is an important factor in determining the resistance observed in the field explaining on average 67% of the observed variation. Adding infection frequency (IF) and urediosorus size (US) to the linear model increased the proportion of the observed variation in the field explained by the components to 80%. This result supports the idea that the components of PR inherit independently, at least, in part.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 60
    Digitale Medien
    Digitale Medien
    Springer
    Plant and soil 120 (1989), S. 95-101 
    ISSN: 1573-5036
    Schlagwort(e): allelopathy ; lyophilization ; Oryza sativa ; phytoxicity ; rhizosphere soil ; Triticum aestivum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Abstract Using a wheat seed bio assay, the phytotoxicity of extracts obtained from wheat and rice rhizosphere soils was determined. The wheat rhizosphere soil extract was found to show phytotoxic effects. More allelopathic materials were found from the wheat-rice rotation soils than from the rice soil. Extracts obtained under basic conditions (pH 8) were found to be more inhibitory than those obtained by acid extraction (pH 5). Wheat straw was found also to contain phytotoxic substances. A comparison of two methods in concentrating the aqueous extracts, rotary evaporation and lyophilization, indicates that the former contained more substances with phytotoxic effects.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 61
    ISSN: 1573-5044
    Schlagwort(e): anther culture ; Triticum aestivum ; donor plant environment ; incubation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Four different growth environments (field, two phytotron greenhouses and one growth chamber) were compared, using two genotypes of spring wheat, one recalcitrant and one responsive. Field-grown plants gave inferior results. Large improvements could be made by improving the conditions, embryoid frequencies in the two genotypes reaching 77.1% and 183.9% per 100 anthers, respectively. High light intensity during the induction phase strongly suppressed induction in both genotypes, but stimulated regeneration of green plants in the recalcitrant genotype, which had the lowest regeneration ability. Weak, diffuse light did not inhibit induction while the positive effect on regeneration was maintained. Also, another recalcitrant genotype was grown in the field, together with two F1-hybrids (recalcitrant x recalcitrant and recalcitrant x responsive). Evidence for a three-factor system was obtained.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 62
    ISSN: 1573-5044
    Schlagwort(e): wheat ; Triticum aestivum ; ABA ; ABA analogs ; somatic embryogenesis ; precocious germination
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Zygotic embryos from ten spring wheat (Triticum aestivum L.) genotypes were tested for embryogenic callus induction in the presence or absence of externally supplied (±)-abscisic acid (ABA) and two of its analogs, methyl abscisate and methyl epoxy-beta-ionylideneacetate. (±)-ABA and its analogs suppressed precocious germination of cultured late-stage embryos and promoted embryogenic callus induction. A significantly greater number of plants was regenerated from calli induced in the presence of ABA and ABA analogs. Early-stage embryos when cultured in the presence of (±)-ABA showed a negative response. Possible roles of ABA with respect to the expression of somatic embryogenesis are discussed.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 63
    Digitale Medien
    Digitale Medien
    Springer
    Plant cell, tissue and organ culture 18 (1989), S. 227-230 
    ISSN: 1573-5044
    Schlagwort(e): sterility ; diploid ; haploid ; pollen plants ; Triticum aestivum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract The fertility of plants from wheat anther culture was studied. It was found that one half of the 36 plants with diploid root tips didn't set seeds at all, and that 41 of the 42 plants with haploid root tips were completely sterile. It was surprising that sterility was so widely distributed even among the plants with diploid root tips.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 64
    Digitale Medien
    Digitale Medien
    Springer
    Journal of comparative physiology 159 (1989), S. 237-242 
    ISSN: 1432-136X
    Schlagwort(e): Amylase ; Mosquitofish ; Rat ; Drosophila ; Structure ; Function
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Medizin
    Notizen: Summary Amylases from the mosquitofish (Gambusia affinis holbrooki, Pisces: Poeciliidae) and rat were purified and compared withDrosophila amylases in terms of structure and function. At the structural level, amino acid compositions of the three amylases were compared. At the functional level, amylase activities were compared on various substrates and in the presence of inhibitors. While the amylases from all three organisms had properties typical of alpha-amylases, both structural and functional differences were observed. Using resemblance coefficients of distance and similarity from numerical taxonomy, it was determined that the amylases from the rat andDrosophila were more similar to each other than either was to amylase from the mosquitofish, and that structural differences between the amylases did not reflect functional differences, i.e. there was no correlation between amylase structural and functional distances.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 65
    Digitale Medien
    Digitale Medien
    Springer
    Journal of chemical ecology 15 (1989), S. 663-676 
    ISSN: 1573-1561
    Schlagwort(e): Drosophila ; Diptera ; Drosophilidae ; triterpene glycosides ; cactus ; fitness components ; host-plant relationships
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract The effects of pentacyclic triterpene glycosides extracted from agria and organ pipe cacti on three fitness parameters of the cactophilic fruit fly,Drosophila mojavensis were tested. Triterpene glycosides from organ pipe increased development time and reduced larval viability while those from agria produced smaller adults (reduced fecundity). In addition, the microbial communities in the organ pipe saponin media were less dense than those in the media to which agria saponins had been added. The role of cactus triterpene glycosides in the ecology of thisDrosophila species is discussed.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 66
    Digitale Medien
    Digitale Medien
    Springer
    European journal of plant pathology 95 (1989), S. 85-105 
    ISSN: 1573-8469
    Schlagwort(e): Triticum aestivum ; Erysiphe graminis ; vertical distribution ; triadimefon ; AUDPC-value ; economic thresshold ; seed number
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Beschreibung / Inhaltsverzeichnis: Samenvatting Opbrengstderving van wintertarwe door meeldauw werd bestudeerd in 11 veldproeven in Nederland. De schade bedroeg gemiddeld 0.013 (SE=0.003) kg are−1 per puistdag meeldauw per blad, vanaf het tweede-knoop stadium tot begin deegrijp bij opbrengstniveaus van 70 tot 90 kg are−1, in de blanco. Bij een ziektestress van 0 tot 2000 puistdagen meeldauw per blad werd geen afwijking van een rechtlijnig verband gevonden. De schaderelatie werd niet significant beïnvloed door de verschillende jaren, rassen of grondsoorten. Het effect van meeldauw op enkele opbrengstcomponenten werd aangetoond. Meeldauwprofielen in de onbehandelde veldjes konden worden beschreven met de vergelijking: CM=CLAb, waarin CM het cumulatieve aantal puistjes is en CLA het cumulatieve bladoppervlak, beide berekend van bovenin het bladerdek naar beneden, de totalen gestandaardiseerd op één. De gradiënt parameter b bedroeg gemiddeld 3.4 (SE=0.9). Waargenomen verschillen in steilte van de meeldauwprofielen beïnvloedde de schaderelatie niet aantoonbaar.
    Notizen: Abstract Damage by mildew to winter wheat was studied in 11 field experiments in the Netherlands. Damage is described by the simple function: −0.013 (SE=0.003) kg are−1 per pustule-day of mildew per leaf, from second node stage to early dough at yield levels of 70 to 90 kg are−1, in diseasefree plots. No deviations from linearity at disease stresses from zero to two thousand pustuledays per leaf were observed. Years, cultivars or soil types did not affect the damage function significantly. The effects of mildew on some yield components were suggested. Mildew profiles in untreated plots could be described by the equation: CM=CLAb, in which CM and CLA are the cumulative pustule number and the cumulative leaf area, respectively, calculated both from top to bottom of the canopy, and totals standardized at unity. Estimates of the gradient parameter b aveaged 3.4 (SE=0.9). Observed differences in steepness of the profiles did not affect the damage function significantly.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 67
    Digitale Medien
    Digitale Medien
    Springer
    Journal of insect behavior 1 (1988), S. 3-15 
    ISSN: 1572-8889
    Schlagwort(e): host preference ; habitat selection ; experience ; learning ; Drosophila ; host races ; population genetics
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract A field experiment with Drosophila melanogasterrevealed that when flies encounter a particular food type soon after emergence, the probability of their subsequently being attracted to such a resource is increased. In this experiment, the length of time flies experienced their postemergence environments was under the control of the flies themselves. The experiment thus realistically mimicked one form of experiential effect that may be important in nature. A theoretical model is developed which shows that enhanced adult preferences for the types of resources fed on as larvae can substantially increase the degree of host-based genetic subdivision within a polyphagous population.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 68
    Digitale Medien
    Digitale Medien
    Springer
    Entomologia experimentalis et applicata 47 (1988), S. 81-88 
    ISSN: 1570-7458
    Schlagwort(e): imaginal diapause ; male mating activity ; genetics ; Drosophila
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Beschreibung / Inhaltsverzeichnis: Résumé Les femelles de D. triauraria Bock & Wheeler (Dipt. Drosophilidae) sont connues pour présenter une diapause reproductrice aux photophases courtes. Les mâles eux aussi ont révélé une activité sexuelle réduite aux photophases courtes, c'est-à-dire qu'ils sont entrés comme les femelles en diapause reproductive. Les photophases critiques pour l'induction de la diapause des mâles et des femelles n'ont pas présenté de différences. Les diapause des mâles et des femelles s'achèvent même sous courtes photophases, mais la diapause mâle était quelque peu plus faible que la diapause femelle. La photophase critique et le taux de diapause ont varié en fonction de l'origine géographique dans l'espèce actuelle. Lors de croisements entre lignées diapausantes et non-diapausantes, la photophase critique et la durée de la diapause ont été héritées quantitativement. A partir de ces expériences et d'expériences précédentes de croisements (Kimura, 1983), quelques modèles de méchnisme d'induction de la diapause de cette espèce sont proposés.
    Notizen: Abstract In Drosophila triauraria Bock & Wheeler (Diptera: Drosophilidae) of which females were known to enter reproductive diapause at short daylengths, males also showed reduced mating activity at short daylengths, i.e., males as well as females entered reproductive diapause. The critical daylength for diapause induction did not differ between females and males. Both male and female diapause ended even under short daylengths, but the male diapause was somewhat weaker than the female diapause. The critical daylength and the diapause rate varied geographically in this species. In the cross between diapausing and non-diapausing strains, the critical daylength and the diapause duration inherited in a quantitative manner. On the basis of the present and previous crossing experiments, some models are proposed on the mechanism of diapause induction of this species.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 69
    Digitale Medien
    Digitale Medien
    Springer
    Biology and fertility of soils 7 (1988), S. 67-70 
    ISSN: 1432-0789
    Schlagwort(e): Root activity ; Soil C mineralization ; 14C-labelled plant material ; Decomposition stages ; Wheat ; Triticum aestivum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Geologie und Paläontologie , Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Two different soils were amended with 14C-labelled plant material and incubated under controlled laboratory conditions for 2 years. Half the samples were cropped with wheat (Triticum aestivum) 10 times in succession. At flowering, the wheat was harvested and the roots removed from the soil, and a new crop was started. Thus, the soil was continuously occupied by predominantly active root systems. The remaining samples were maintained without plants under the same conditions. The aim of the experiment was to study the effects of active roots on C-mineralization rates during different stages of decomposition and during long-term incubation. During the first 200 days, corresponding to the active decomposition stages, the roots weakly reduced 14C mineralization. With a lower level of decomposition, when more than 60% of the initial 14C was mineralized and when the available nutrients were markedly exhausted by plant uptake, the roots stimulated 14C mineralization.[/ p]
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 70
    Digitale Medien
    Digitale Medien
    Springer
    Biology and fertility of soils 7 (1988), S. 71-78 
    ISSN: 1432-0789
    Schlagwort(e): Root activity ; Rhizosphere ; C metabolism ; Microbial biomass ; Microbial activity ; Wheat ; Triticum aestivum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Geologie und Paläontologie , Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary Two different soils were amended with 14C-labelled plant material and incubated under controlled laboratory conditions for 2 years. Half the samples were cropped with wheat (Triticum aestivum) 10 times in succession. At flowering, the wheat was harvested and the old roots removed from the soil, so that the soil was continuously occupied by predominantly active root systems. The remaining samples were maintained without plants under the same conditions. During the initial stages of high microbial activity, due to decomposition of the labile compounds, the size of the total microbial biomass was comparable for both treatments, and the metabolic quotient (qCO2-C = mg CO2-C·mg−1 Biomass C·h−1) was increased by the plants. During the subsequent low-activity decomposition stages, after the labile compounds had been progressively mineralized, the biomass was multiplied by a factor of 2–4 in the presence of plants compared to the bare soils. Nevertheless, qCO2-C tended to reach similar low values with both treatments. The 14C-labelled biomass was reduced by the presence of roots and qCO2-14C was increased. The significance of these results obtained from a model experiment is discussed in terms of (1) the variation in the substrate originating from the roots and controlled by the plant physiology, (2) nutrient availability for plants and microorganisms, (3) soil biotic capacities and (4) increased microbial turnover rates induced by the roots.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 71
    Digitale Medien
    Digitale Medien
    Springer
    Biology and fertility of soils 6 (1988), S. 315-321 
    ISSN: 1432-0789
    Schlagwort(e): Biopores ; Root growth ; Transpiration ; Simulations ; Mechanical impedance ; Triticum aestivum
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Geologie und Paläontologie , Land- und Forstwirtschaft, Gartenbau, Fischereiwirtschaft, Hauswirtschaft
    Notizen: Summary The use of vertical biopores by wheat (Triticum aestivum) seminal roots for easy access to the subsoil and the consequences for plant water supply and yield has been investigated by computer simulation. Parameters included were: biopore density and diameter, depth of cultivation and strength of the subsoil — all under a wide range of seasonal weather conditions. The model predicts that biopores add significantly to root penetration at depth, even at a density of 0.1% v/v of small, vertical pores, while 1.5% to 2.0% v/v can ensure maximum root penetration. When the growing season is shorter a larger number of biopores is needed to ensure timely root penetration to depth. With shallow tillage, biopores occur closer to the soil surface, and their importance is increased. Deeper root penetration invariably gives greater water uptake and transpiration, but may have a negative effect on grain yield, especially under the driest climatic conditions. An increase in early water use may result in less soil water being available during the grain-filling period. The effect of biopores on plant transpiration varies from year to year, depending on the amount of rain and its distribution in time, and on the amount of soil water stored at time of sowing.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 72
    ISSN: 1432-0983
    Schlagwort(e): Triticum aestivum ; Plant mitochondrial DNA ; Ribosomal genes ; Sequence rearrangements
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The nucleotide sequence of the wheat mitochondrial 26S ribosomal RNA gene and flanking regions was determined and compared with mitochondrial 26S rRNA genes from maize and Oenothera. All three genes exhibit a high degree of homology except within two variable regions. When the plant mitochondrial 26S rRNA genes are compared with Escherichia coli 23S rRNA and chloroplast 23S and 4.5S rRNA genes, a third variable region is apparent close to the 3′ end of the gene. The 5′ and 3′ ends of the wheat mitochondrial gene were determined by S1 nuclease mapping. Computer analysis of the wheat mitochondrial gene revealed several small sequences present either in the 5′ region of the 26S rRNA gene or in the 18S rRNA gene.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 73
    ISSN: 1432-0983
    Schlagwort(e): Triticum aestivum ; Genomic variability ; Mitochondrial DNA ; Somatic tissue culture ; Regeneration ability
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Embryogenic and non-embryogenic long-term callus cultures of hexaploid wheat exhibit differences in the organization of their mitochondrial genome. Embryogenic and non-embryogenic fractions of callus cultures initiated from immature embryos of the wheat cultivar “Chinese Spring” have been isolated and subsequently subcultured. DNA-DNA hybridization experiments using labelled cloned wheat mitochondrial DNA fragments have shown that the mitochondrial DNA organization of embryogenic subcultures derived from embryogenic parts of “Chinese Spring” calli is closely related to that of the initial “Chinese Spring” calli, while non-embryogenic subcultures derived from non-embryogenic fragments of “Chinese Spring” calli exhibit a mitochondrial DNA organization similar to that found in non-embryogenic calli derived from cultivar “Aquila”. In addition, somatic tissue cultures initiated from three other non-embryogenic wheat cultivars (“Talent”, “Thésée” and “Capitole”) display mitochondrial DNA arrangements similar to those found in cultivar “Aquila”. These results strongly suggest that, in wheat callus cultures, a particular mitochondrial genome organization is correlated with the ability of cultured cells to regenerate whole plants.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 74
    Digitale Medien
    Digitale Medien
    Springer
    Journal of molecular evolution 28 (1988), S. 145-150 
    ISSN: 1432-1432
    Schlagwort(e): Alcohol dehydrogenase ; Drosophila ; Enzyme kinetics ; Product inhibition ; Microevolution
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Because natural populations ofDrosophila melanogaster are polymorphic for different allozymes of alcohol dehydrogenase (ADH) and becauseD. melanogaster is more tolerant to the toxic effects of ethanol than its sibling speciesD. simulans, information regarding the sensitivities of the different forms of ADH to the products of ethanol degradation are of ecological importance. ADH-F, ADH-S, ADH-71k ofD. melanogaster and the ADH ofD. simulans were inhibited by NADH, but the inhibition was relieved by NAD+. The order of sensitivity of NADH was ADH-F〈ADH-71k, ADH-S〈ADH-simulans with ADH-F being about four times less sensitive than theD. melanogaster enzymes and 12 times less sensitive than theD. simulans enzyme. Acetaldehyde inhibited the ethanolto-acetaldehyde activity of the ADHs, but at low acetaldehyde concentrations ethanol and NAD+ reduced the inhibition. ADH-71k and ADH-F were more subject to the inhibitory action of acetaldehyde than ADH-S and ADH-simulans, with ADH-71k being seven times more sensitive than ADH-S. The pattern of product inhibition of ADH-71k suggests a rapid equilibrium random mechanism for ethanol oxidation. Thus, although the ADH variants only differ by a few amino acids, these differences exert a far larger impact on their intrinsic properties than previously thought. How differences in product inhibition may be of significance in the evolution of the ADHs is discussed.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 75
    Digitale Medien
    Digitale Medien
    Springer
    Development genes and evolution 197 (1988), S. 19-26 
    ISSN: 1432-041X
    Schlagwort(e): Drosophila ; Morphogenesis ; Appendages
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The phenotype of rotund (rn) null alleles is described, and compared to wild type. The mutants are expressed zygotically and cause position specific defects in certain imaginal discs (antenna, legs, wing, haltere and proboscis) and their corresponding adult derivatives. In the discs, specific folds are absent in rn mutants compared to wild type. Clonal analysis shows that the rn + gene is partially autonomous in its expression in cells destined to form certain distal parts of the adult appendages. The results are consistent with the idea that the rn + gene is required for normal morphogenesis of specific distal parts of the adult appendages.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 76
    Digitale Medien
    Digitale Medien
    Springer
    Development genes and evolution 197 (1988), S. 40-48 
    ISSN: 1432-041X
    Schlagwort(e): Drosophila ; Homeosis ; Tumorous-head ; Bithorax-complex mutant ; Morphology
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Transformations of tumorous-head Drosophila melanogaster were examined in order to investigate whether head structures were replaced by specific abdominal structures. Heads selected for the presence of genital structures were analyzed in detail. Female abnormalities included any combination of vaginal teeth, vulvar papillae, sensilla trichodea, abdominal tergites 6 (T6), 7 (T7), 8 (T8) and anal plate. Anal plate was observed in the prefrons and rostral membrane, while all other genital structures were intimately associated with modified shingle cuticle. Male abnormalities included transformation of antennal structures to penis, clasper teeth, lateral plate, anal plate and eye to T6. The distribution of each type of homeotic structure was confined to general regions of the eye-antenna, with no precise dividing lines between them. However, the spatial sequence of homeotic structures in the eye-antenna was generally the same as the sequence of the same structures in the posterior abdomen.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 77
    Digitale Medien
    Digitale Medien
    Springer
    Development genes and evolution 197 (1988), S. 457-470 
    ISSN: 1432-041X
    Schlagwort(e): Neurogenesis ; Neurogenic genes ; Achaetescute complex ; Daughterless ; Genetic interactions ; Drosophila
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary In Drosophila melanogaster the neuroblasts separate from epidermoblasts to give rise to the neural primordium. This process is under the control of several genes. The group of the so-called neurogenic genes is required for epidermal development; other genes, comprising those of the achaete-scute complex and daughterless, are required for neural development. We have studied the relationships between both groups of genes in two different ways. We have analyzed the phenotype of double-mutant embryos and our results show that the neural hyperplasia caused by neurogenic mutations can be partially prevented if a mutation in one of the other genes is present in the same genome. Only the neural cells that do not require the function of a particular gene of the achaete-scute complex in the wild-type seem to develop to a neural fate in the double mutant embryos. At least some of the genetic interactions affect the transcriptional level, as shown by in situ hybridization, since the territories of transcription of the achaetescute genes are expanded in neurogenic mutants. All cells of the neurogenic region of the double mutants apparently initiate neural development. However, during later development some of these cells switch their fate either to epidermogenesis or to cell death and this leads to the final phenotype of the double mutants. We discuss these results with respect to the events of early neurogenesis.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 78
    ISSN: 1432-041X
    Schlagwort(e): Drosophila ; shibire ; Neuronal development ; Muscle ; Giant fiber pathway
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The temperature-sensitive mutation shibire (shi) in Drosophila melanogaster is thought to disrupt membrane recycling processes, including endocytotic vesicle pinch-off. This mutation can perturb the development of nerves and muscles of the adult escape response. After exposure to a heat pulse (6 h at 30° C) at 20 h of pupal development, adults have abnormal flight muscles. Wing depressor muscles (DLM) are reduced in number from the normal six to one or two fibers, and are composed of enlarged fibers that appear to represent fiber fusion; large spaces devoid of muscle fibers suggested fiber deletion. The normal five motor axons are present in the peripheral nerve PDMN near the ganglion. However, while some motor axons pass dorsally to the extant fibers, other motor axons lacking end targets pass into an abnormal posterior branch and terminate in a neuroma, i.e., a tangle of axons and glia without muscle target tissue. Hemisynapses are common in axons of the proximal PDMN and within the neuroma, but they are rarely seen in control (no heat pulse) shi or wild-type flies. All surviving muscle fibers are innervated; no muscle tissue exists without innervation. Fibrillar fine structure and neuromuscular synapses appear normal. Fused fibers have dual innervation, suggesting correct and specific matching of target tissue and motor axons. Motor axons lacking target fibers do not innervate erroneous targets but instead terminate in the neuroma. These results suggest developmental constraints and rules, which may contribute to the orderly, stereotyped development in the normal flight system. The nature of the anomalies inducible in the flight motor system in shi flies implies that membrane recycling events at about 20 h of pupal development are critical to the formation of the normal adult nerve-muscle pattern for DLM flight muscles.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 79
    Digitale Medien
    Digitale Medien
    Springer
    Development genes and evolution 197 (1988), S. 413-418 
    ISSN: 1432-041X
    Schlagwort(e): Cell interactions ; Cell commitment ; Neurogenesis ; Drosophila
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Cell-cell interactions are involved in mediating developmental fate. An example is the decision of the neuroectodermal cells of Drosophila to develop as neural or epidermal progenitors, where cellular interactions participate in the process of acquisition of either cell fate. The results of heterochronic cell transplantations we describe here suggest that both neuroblasts and epidermoblasts are not irreversibly committed to a particular developmental fate. Rather, they retain the ability to interact with neighbouring cells and, under our experimental conditions, are capable of switching their fate during a relatively long period of time, i.e. until the end of embryonic stage 11.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 80
    Digitale Medien
    Digitale Medien
    Springer
    Development genes and evolution 197 (1988), S. 435-440 
    ISSN: 1432-041X
    Schlagwort(e): Drosophila ; Homoeotic genes ; Segment differentiation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Regulator of bithorax (Rg-bx)−, or trithorax (trx)− lethal larvae occasionally show a homoeotic transformation of the dorsal prothorax to mesothoracic structures. This transformation suggests a reduced activity of the Sex combs reduced (Scr) gene on the basis of gene dosage studies, as well as enhanced expression of the phenotypes of the weak Scr − alleles in Rg-bx − larvae. Morphological observations of adult flies doubly heterozygous for Rg-bx and Scr mutations also suggest the enhancement of an aspect of Scr adult phenotypes. I conclude that the Rg-bx + gene function is required for the optimal expression of the Scr gene in larval and imaginai cells.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 81
    Digitale Medien
    Digitale Medien
    Springer
    Development genes and evolution 197 (1988), S. 507-512 
    ISSN: 1432-041X
    Schlagwort(e): Drosophila ; Reporter gene ; hairy ; Segmentation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Random insertions of a promotor fused to a reporter gene, such as Lac-Z, reveal regulatory sequences that confer temporal and spatial patterns of gene expression in eukaryotes. These patterns may reflect the activity of a neighbouring gene and thus lead to the isolation of new genes essential for normal development. Here, we demonstrate that this hypothesis is true for an insertion into the well characterized segmentation gene, hairy, in Drosophila. The insertion is homozygous lethal and fails to complement other hairy alleles, giving the phenotype described for hairy mutations. The insertion is located at 66D on the polytene chromosome map, is within 300–600 bp 5′ to the first hairy exon, and is orientated in the same sense (5′-3′) as the hairy transcription unit. Expression of β-galactosidase (β-gal), deriving from the insertion, follows closely the spatio-temporal patterns of expression of hairy gene product during embryogenesis. In addition, other sites of β-galactosidase expression are shown in the third larval instar stage and in the adult ovary. The results show that some insertions, giving restricted patterns of reporter gene expression, will reflect the temporo-spatial activity of a nearby gene.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 82
    ISSN: 1432-041X
    Schlagwort(e): Drosophila ; Injection of embryo ; Daudi conditioned medium ; Rudimentary ovaries ; Mean number of ovarioles
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Drosophila melanogaster embryos were injected before the blastoderm stage with conditioned media from several male Burkitt's lymphoma human cell lines and the Daudi cell line. Such injections do not have any effect on the male genital apparatus or on the female tract. The Daudi conditioned medium modifies the ovarian morphogenesis of the flies and the rudimentary ovaries obtained look like nymphal gonads. Moreover, they have a drastically reduced number of germ cells. The ovaries that looked functional contain numerous necrotic germ cells and the mean number of ovarioles per fly is significantly smaller than that of the controls. The abnormalities observed resemble the results of experimental and genetic lack of germ cells. They disappear at very high dilution (1×10−6).
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 83
    Digitale Medien
    Digitale Medien
    Springer
    Development genes and evolution 197 (1988), S. 231-238 
    ISSN: 1432-041X
    Schlagwort(e): Drosophila ; Electrical polarity ; Ovarian follicles
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Distribution of rhodamine-conjugated lysozyme injected into the sixteen-cell syncytium comprising the germ-line portion of theDrosophila follicle is shown to be affected by charge. Positive molecules are able to migrate through intercellular bridges from the oocyte to the nurse cells, but are unable to migrate detectably from nurse cells to the oocyte. Their negatively charged counterparts can move from the nurse cells to the oocyte, but are unable to traverse the intercellular bridges in the counter direction. This charge-dependent movement of molecules is accompanied by an electrical potential difference, focused across the nurse cell-oocyte bridges, which makes the nurse cells negatively charged to the oocyte. The addition of insect hemolymph to the physiological salt solution in which the experiments were performed resulted in only a small increase in the transmembrane resistance, but enhanced the potential difference between oocyte and nurse cells from 0.2±0.3 (SE) mV (nurse cells negative) to 2.3±0.45 (SE) mV (nurse cells negative).
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 84
    ISSN: 1432-041X
    Schlagwort(e): Drosophila ; Homeosis ; Epidermal development ; Embryology ; Clonal analysis
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The complex genetic locuspolyhomeotic (ph) is a member of thePolycomb (Pc)-group of genes and as such is required for the normal expression of ANT-C and BX-C genes. It also has probably other functions since amorphicph alleles display a cell death phenotype in the ventral epidermis of 12-h-old embryos. Here it is shown that lethal alleles ofph (amorph and strong hypomorph) show transformation of most of their segments towards AB8. Theph + product is required autonomously in imaginal cells. The total lack ofph + function prevents viability of the cuticular derivatives of these cells.ph has a strong maternal effect on segmental identity and epidermal development that can not be rescued by one paternally supplied dose ofph + in the zygote. These phenotypes differ substantially from those of previously describedPc-group genes. AmongPc-group genes,ph seems to be the only one that is strongly required both maternally and zygotically for normal embryonic development.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 85
    ISSN: 1432-041X
    Schlagwort(e): Drosophila ; Ultrabithorax ; Development ; Regulation ; Protein distribution
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Most viable alleles of homeotic genes cause partial transformations within given lineages in a topographically specific fashion. We study this phenomenon as a way to understand the normal mechanisms involved in the spatial regulation of homeotic gene expression. To this end we have investigated the distribution of Ultrabithorax (Ubx) proteins in imaginai discs mutant for hypomorphic and neomorphic alleles of Ubx and alleles of trans-acting genes. We find that the morphological discontinuities observed in the adult transformations are caused by corresponding new patterns of the Ubx proteins in the imaginai anlagen. These novel patterns of Ubx proteins are understood as a consequence of a process of reinforcement-extinction of Ubx expression. The evidence suggesting that this process results from a positive feed-back loop and cell-cell interactions is discussed.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 86
    ISSN: 1432-041X
    Schlagwort(e): Bithorax complex ; Drosophila ; Abdominal-B mutations ; Genetic mosaics
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The phenotypic effects in imaginal hypodermal tissue of a number of Abdominal-B mutations of the bithorax complex are described. Evidence is given from complementation analysis that the phenotypic heterogeneity in both the spatial limits and the nature of the homeotic transformations produced is not an arbitrary classification of allelic differences that we find. We have used genetic mosaic analysis to support the interpretation that the Abdominal-B genetic unit can exist in a number of alternative functional states of expression during development and that individual Abdominal-B mutations may abolish some states whilst leaving others unaffected.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 87
    ISSN: 1432-041X
    Schlagwort(e): Drosophila ; Segment polarity gene ; Maternal effect
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Fused is a segmentation gene belonging to the segment-polarity class. Mutations at thefused locus are known to display pleiotropic effects, causing zygotically determined anomalies of ovaries and of some adult cuticular structures, and maternally determined embryonic segmentation defects. In order to determine the amorphic phenotype offused and to study the genetical basis of its pleiotropy, newfused alleles (18 viable and 11 lethal) were isolated. The phenotype of these mutants and of others already known are described, taking into account zygotic and maternal effects. The main results provided by this analysis are as follows. Firstly, allfused alleles show the whole complex fused phenotype, and a good correlation is observed between the strength of the wing and segmentation defects, suggesting that a single function is involved in both processes. Secondly, all embryonic and larval lethals carry deficiencies which allow us to localizefused between the 17C4 and 17D2 bands of the X-chromosome. Thirdly, the 24 viable and 2 pupal lethals examined behave as point mutants, as shown cytologically or by Southern blot analysis. However, only one of them, the pupal lethalfu mH63 was proven to carry a nullfused allele, since it displays in germ-line clones a strong maternal phenotype and a very low zygotic rescue, similar to those of the small deficiencyDf(1)fu z4. The phenotype of the amorphic mutant indicates that zygotic ezpression offused is required for normal metamorphosis, while maternal expression is necessary for a normal segmentation pattern, since a complete loss offused expression during oogenesis cannot be compensated zygotically.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 88
    Digitale Medien
    Digitale Medien
    Springer
    Development genes and evolution 197 (1988), S. 75-91 
    ISSN: 1432-041X
    Schlagwort(e): Drosophila ; Maternal effect mutation ; Pattern formation ; Gastrulation
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary The gastrulation defective (gd) locus is a maternally expressed gene in Drosophila required for normal differentiation of structures along the embryonic dorso-ventral axis. Cuticular defects of the offspring from females with different combinations of gd alleles comprised a phenotypic continuum. Complementation among several alleles produced normal offspring while progressively more severe mutations produced a graded loss of structures from ventral, and then lateral, blastoderm cells. The most severely affected embryos consisted entirely of structures derived from dorsal blastoderm cells. Histological examination of staged siblings from selected allelic combinations showed that internal tissues were similarly affected. The tissues observed in amorphic embryos support new, more dorsal, assignments of fate map positions for blastoderm precursors of the cephalopharyngeal apparatus, hindgut and ventral nerve cord. The loss of ventral and lateral structures did not occur through cell death and appeared to involve a change in blastoderm cell fate. A direct effect of the mutations on blastoderm cell determination, however, was insufficient to explain the development of the dorsalized embryos. Intermediate phenotypes suggested that cell interactions or movements associated with morphogenesis are required for the determination of some cell fates in the dorsoventral axis. Thus, the developmental fate of all blastoderm cells may not be fixed at the time of blastoderm formation.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 89
    Digitale Medien
    Digitale Medien
    Springer
    Development genes and evolution 197 (1988), S. 115-123 
    ISSN: 1432-041X
    Schlagwort(e): X-Chromosome Loci ; Dominant maternal effect ; Segmentation genes ; Drosophila
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary A systematic search for X chromosome loci showing a dominant maternal interaction with the segmentation genes Krüppel, hunchback, knirps and hairy was performed using deficiencies spanning 65% of the X chromosome. No interaction with the knirps gene was observed, but five regions of the X chromosome showed a maternal dominant interaction with the Krüppel gene. Two of these regions also show a maternal dominant interaction with either hunchback (region 10A7–10A8) or hairy (region 10E1–10F3). In all of these interactions dead embryos were observed which showed the same defects as embryos homozygous for the segmentation gene tested. These results suggest that a complex repartition of maternal products necessary for subsequent segmentation may occur in the Drosophila egg.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 90
    Digitale Medien
    Digitale Medien
    Springer
    Oecologia 75 (1988), S. 400-404 
    ISSN: 1432-1939
    Schlagwort(e): Vectoring ; Drosophila ; Cactophilic yeasts ; Dispersal ; Community structure
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary At two locations in the Sonoran Desert, yeasts were sampled from species of Drosophila, the flies' cactus hosts, and other neighboring sources of cactophilic yeasts to determine the relation between the yeasts vectored by the fly and the yeasts found in their breeding sites. D. mojavensis, D. nigrospiracula, and D. mettleri vectored yeast assemblages significantly more similar to the yeast species found on the rot from which the flies were collected than to the yeasts found on other rots from the flies host cactus or other rotting cactus at the same site. Rots with Drosophila had fewer yeast species than those without flies, suggesting that flies were associated with younger rots. Rots with flies and the Drosophila also had more yeast species with the capability to produce ethyl acetate than rots without flies. The results support the contention that cactophilic Drosophila feed on a subset of the yeasts available in an area, and may act to maintain differences among the yeast communities found on different species of cactus.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 91
    Digitale Medien
    Digitale Medien
    Springer
    Oecologia 75 (1988), S. 516-520 
    ISSN: 1432-1939
    Schlagwort(e): Drosophila ; Picornavirus ; Fertility ; Embryonic and larvo-pupal death rate
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Drosophila C virus (DCV) has a considerable impact on ovarian morphogenesis inDrosophila melanogaster host populations. This virus also affects the developmental time and the fresh weight of infected females. In order to investigate the hypothesis that DCV may play a role in the dynamics ofDrosophila populations, the fertility and embryonic and larvo-pupal death rates of a host population and that of five DCV-free populations were determined. A comparison of two populations, one of them DCV-free, the other infected, suggested that the fertility of the DCV-infected flies was higher than that of uninfected flies, despite a greater larvo-pupal death rate. Fertility of the infected flies was greater among the infected population than for the DCV-free populations. The DCV-free populations originated from five different localities. The virus clearly does have an impact on the biotic potential of its host population. This paper reports for the first time a positive interaction between a viral population and a host population as it increases certain parameters of host population dynamics.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 92
    Digitale Medien
    Digitale Medien
    Springer
    Sexual plant reproduction 1 (1988), S. 217-222 
    ISSN: 1432-2145
    Schlagwort(e): Triticum aestivum ; Cytology ; In vitroandrogenesis ; Fluorescent microscopy
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Summary Two winter wheat genotypes (‘Diószegi 200’ and ‘Mv 15’) were compared for their in vitro androgenic capacity. On average, the induction frequency of embryogenic structures was 71.7% in ‘Diószegi 200’ and only 4.3% in ‘Mv 15’. The haploid induction ability of the two genotypes differed considerably, with ‘Diószegi 200’ being much higher. The difference in the in vitro inductability of the microspores may result from genetic differences which are manifested in the survival rate of the microspores during the culture period and their adaptability to in vitro conditions. Special DNA fluorochrornes were suitable for studying the different pathways of in vitro androgenesis. Our data indicate that the repeated equal divisions of the microspore nucleus might lead to pollen embryo formation, and subsequent divisions of the vegetative portion of the pollen grain after the first asymmetric microspore mitosis can result in pollen callus formation.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 93
    Digitale Medien
    Digitale Medien
    Springer
    Biochemical genetics 26 (1988), S. 131-141 
    ISSN: 1573-4927
    Schlagwort(e): Drosophila ; arginine kinase ; mitochondria ; isozymes
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Mitochondrial and cytoplasmic isozymes of arginine kinase have been identified inDrosophila melanogaster. On the basis of their immunological similarity, parallel dosage responses, and cosegregation of electrophoretic mobility differences, it is concluded that both isozymes are the product of a single gene. The consequences of this in relation to the regulation and evolution of this unusual gene-enzyme system are discussed. It is inferred that the origin of the phosphagen shuttle must predate the divergence of invertebrates and vertebrates.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 94
    Digitale Medien
    Digitale Medien
    Springer
    Biochemical genetics 26 (1988), S. 783-803 
    ISSN: 1573-4927
    Schlagwort(e): Drosophila ; dipeptidase activities ; genetic variation ; activity modifiers
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract An examination ofDrosophila melanogaster from natural populations revealed genetic variation for dipeptidase-A (DIP-A) and dipeptidase-B (DIP-B) activities within sets of lines that differed from one another only in the second or the third chromosome. Analyses of diallel crosses indicate that both activities are inherited additively, and coordinate control of expression is suggested by the significant positive correlation between the two activities. Electrophoresis and thermal denaturation studies failed to detect structural differences among lines with different levels of DIP-A activity. No characteristic level of activity could be associated with any DIP-A allozyme. Mapping experiments revealed the presence of activity modifiers that are in tight linkage with the structural gene, as well as those that manifest their effects from a distance. The maximum genetic distance between a high-activity effect on DIP-A and the structural gene was determined to be 0.029 map unit. These results are in accordance with the prevalence of activity modifiers for various enzymes inDrosophila melanogaster.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 95
    ISSN: 1573-4927
    Schlagwort(e): glutamine synthetase I ; genetic mapping ; allozymes ; null alleles ; Drosophila
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Recombinational and deletion mapping of electrophoretic variants of the glutamine synthetase I isozyme (GSI) inDrosophila melanogaster locates the gene in the 21B region on the second chromosome. We have conducted a genetic analysis of the region extending cytologically from 21A to 21B4-6. Recessive lethal mutations were generated by ethyl methanesulfonate (EMS) and ethyl nitrosourea (ENU) mutagenesis and by hybrid dysgenesis (HD). These lethals fall into seven functional groups, which were partially ordered by complementation with cytologically defined deficiencies of this region generated by hybrid dysgenesis. Two of the EMS- and two of the ENU-induced lethals fulfill biochemical criteria expected for null alleles of the GSI gene.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 96
    Digitale Medien
    Digitale Medien
    Springer
    Biochemical genetics 26 (1988), S. 783-803 
    ISSN: 1573-4927
    Schlagwort(e): Drosophila ; dipeptidase activities ; genetic variation ; activity modifiers
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract An examination ofDrosophila melanogaster from natural populations revealed genetic variation for dipeptidase-A (DIP-A) and dipeptidase-B (DIP-B) activities within sets of lines that differed from one another only in the second or the third chromosome. Analyses of diallel crosses indicate that both activities are inherited additively, and coordinate control of expression is suggested by the significant positive correlation between the two activities. Electrophoresis and thermal denaturation studies failed to detect structural differences among lines with different levels of DIP-A activity. No characteristic level of activity could be associated with any DIP-A allozyme. Mapping experiments revealed the presence of activity modifiers that are in tight linkage with the structural gene, as well as those that manifest their effects from a distance. The maximum genetic distance between a high-activity effect on DIP-A and the structural gene was determined to be 0.029 map unit. These results are in accordance with the prevalence of activity modifiers for various enzymes inDrosophila melanogaster.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 97
    Digitale Medien
    Digitale Medien
    Springer
    Biochemical genetics 26 (1988), S. 527-541 
    ISSN: 1573-4927
    Schlagwort(e): Drosophila ; glue proteins ; glycosylation ; Chromosomal linkage
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Larval glue protein fractions ofDrosophila nasuta nasuta were analyzed by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. Seven major and at least four minor glue protein fractions were recognized. Six of the major fractions are glycosylated. They migrate as three prominent doublets (〉100, 43, and 30/28 kd). The synthesis of traceable amounts of these major fractions begins already during the second as well as during the early stages of the third larval instar. The 43-kd and the 30/28-kd fractions are coded by X-chromosomal genes. They are probably clustered within the huge puff of division 10, which is the most prominent X-chromosomal puff in the polytene chromosomes of the third larval instar. Complex posttranslational modification of all but one major glue protein fraction (14 kd) leads to the formation of about 15 different protein fractions in the final glue product. The amount of glue protein produced byD. n. nasuta larvae (in relation to the total saliva proteins) is nearly twice the amount produced byD. melanogaster larvae (ca. 55 and 32%, respectively).
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
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  • 98
    ISSN: 1573-4927
    Schlagwort(e): glutamine synthetase I ; genetic mapping ; allozymes ; null alleles ; Drosophila
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Recombinational and deletion mapping of electrophoretic variants of the glutamine synthetase I isozyme (GSI) inDrosophila melanogaster locates the gene in the 21B region on the second chromosome. We have conducted a genetic analysis of the region extending cytologically from 21A to 21B4-6. Recessive lethal mutations were generated by ethyl methanesulfonate (EMS) and ethyl nitrosourea (ENU) mutagenesis and by hybrid dysgenesis (HD). These lethals fall into seven functional groups, which were partially ordered by complementation with cytologically defined deficiencies of this region generated by hybrid dysgenesis. Two of the EMS- and two of the ENU-induced lethals fulfill biochemical criteria expected for null alleles of the GSI gene.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
    BibTip Andere fanden auch interessant ...
  • 99
    Digitale Medien
    Digitale Medien
    Springer
    Molecular and cellular biochemistry 79 (1988), S. 181-189 
    ISSN: 1573-4919
    Schlagwort(e): zerknullt gene ; homeobox protein ; Drosophila
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie , Medizin
    Notizen: Summary The zen protein is encoded by the zerknullt gene required for normal early development inDrosophila. Like many regulatory proteins of this type, zen contains a 60 amino acid homeobox sequence. We have purified the zen protein and studied its solution behavior and its interaction with DNA. The zen protein exists as a monomer in solution with a molecular weight of about 40000. It binds specifically to a site about 900 bases upstream from thezen gene. Within this binding site DNase protection experiments indicate that binding is confined to two regions approximately 11 and 14 bases in length that are separated by about 30 base pairs. The protein concentration dependence of the binding curve suggests that protein binding is non cooperative.
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
    BibTip Andere fanden auch interessant ...
  • 100
    Digitale Medien
    Digitale Medien
    Springer
    Biochemical genetics 26 (1988), S. 527-541 
    ISSN: 1573-4927
    Schlagwort(e): Drosophila ; glue proteins ; glycosylation ; Chromosomal linkage
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie , Chemie und Pharmazie
    Notizen: Abstract Larval glue protein fractions ofDrosophila nasuta nasuta were analyzed by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis. Seven major and at least four minor glue protein fractions were recognized. Six of the major fractions are glycosylated. They migrate as three prominent doublets (〉100, 43, and 30/28 kd). The synthesis of traceable amounts of these major fractions begins already during the second as well as during the early stages of the third larval instar. The 43-kd and the 30/28-kd fractions are coded by X-chromosomal genes. They are probably clustered within the huge puff of division 10, which is the most prominent X-chromosomal puff in the polytene chromosomes of the third larval instar. Complex posttranslational modification of all but one major glue protein fraction (14 kd) leads to the formation of about 15 different protein fractions in the final glue product. The amount of glue protein produced byD. n. nasuta larvae (in relation to the total saliva proteins) is nearly twice the amount produced byD. melanogaster larvae (ca. 55 and 32%, respectively).
    Materialart: Digitale Medien
    Standort Signatur Erwartet Verfügbarkeit
    BibTip Andere fanden auch interessant ...
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