ISSN:
1432-072X
Keywords:
Cyanobacteria
;
Anacystis
;
Anoxygenic photosynthesis
;
Reducing agents
;
Electron donors
;
Anaerobiosis
;
Evolution
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Photosynthesis by Anacystis nidulans was studied in presence of reduced sulfur or nitrogen compounds, or of hydrogen. O2 evolution and CO2 fixation were depressed by sulfide, sulfite, cysteine, thioglycollate, hydroxylamine and hydrazine. Sulfite, cysteine and hydrazine inhibited O2 evolution much more strongly than CO2 fixation, indicating ability to supply electrons for CO2 photoreduction; DCMU suppressed these photoreductions. In contrast, some anoxygenic photosynthetic CO2 fixation insensitive to DCMU was found with sulfide, thiosulfate and hydrogen. Emerson enhancement studies confirmed that sulfite, cysteine and hydrazine acted on photosystem II, while photoreduction supported by sulfide, thiosulfate and hydrogen needed photosystem I only. Sulfite was photooxidized to sulfate, sulfide to elemental sulfur, and thiosulfate to sulfate plus elemental sulfur; the sulfur accumulated inside the cells. Results on the stoichiometries of the photoreductions were consistent with the photooxidation products determined. Inhibitor studies suggested photosynthetic CO2 fixation through the Calvin cycle. While photoreduction by all reductants used was found to be constitutive in Anacystis, the process was stimulated by anaerobic preincubation with the reductants only in the cases of hydrogen and thiosulfate; this adaptation was prevented by chloramphenicol and by O2. Anaerobic photoautotrophic growth of Anacystis was, however, not observed; the increase in dry weight with H2 and thiosulfate was not accompanied by cell multiplication or by an increase in chlorophyll content. Parallel short-term experiments with Chlorella did not reveal any constitutive photoreduction in this eukaryotic alga.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00405412
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